RLG00000011659

NAD(P)H-quinone oxidoreductase subunit M

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0016132
Physical Location & Seq
Reverse (-)
11461837 .. 11464116
2280 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011659

Sequence Viewer

Length: 651 bp
ATGCTACCACATTCAGCCATGGCAGCGACTTCTTCTTATATGGCTTGTGCAAAATTCCCTATGTTGGGTTATGCTGGAAGAAGAATCCAACTGAGAAGCAGAAGAGACTTCTTTACAAGAGCTCAGCAGCAACCAGGTGAAGTCCAAGAACCACAAGAACTGAAAATACAAGAACCAGAAGTACAGAGAGAAAAAGGAGAGCCAAAAGGAACAGTGCCAAGGCCAGTGGAGACACAATTGAATGTGAAGAGTAGGAACATGGCGAGAGAATATGGTGGGGATTGGCTTAGCTGTGCCACAAGGCATGTAAGGATTTATGCAGCTTATATAGACCCAGAAACCTGTGCATTCGATCAGACACAGATGGATAAGCTCACCATTATACTTGATCCAACCAATGAGTTTGTGTGGACTCCAGAGACTACCAACAAGGTCTACTCCTACTTCCAAGAGCTTGTGGATCACTATGAGGGTGCAGATTTGACAGAATACACTCTGCGTCTGATTGGTTCAGACATAGAGCACTACATAAGGAAGCTTCTCTATGATGGAGAAATCAAATACAACATGGATGCAAGAGTCCTTAACTTCAGCATGGGTAAGCCTCGCGTTATGTTCAACAACAACGAATTTCAACCTCAGGATGTATGA

Protein Analysis

217

Amino Acids

25.12

Weight (kDa)

5.66

Isoelectric Point (pI)

50.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NdhM PF10664 96 - 204 2.1e-46 Cyanobacterial and plastid NDH-1 subunit M
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016132)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G37925
fragaria_vesca FvH4_2g27960
malus_domestica MD15G1133300.v1.1
prunus_persica Prupe.1G488900_v2.0.a1
pyrus_communis pycom15g12020
rosa_chinensis RchiOBHm_Chr6g0296781
rosa_laevigata RLG00000011659
rosa_multiflora Rmu_ssc0000192.1_g000012
rosa_roxburghii Rroxscaffold_7G00171170
rosa_rugosa Rorug06G0266100 Rorug06G0266200
rosa_samantha Rh6AG377200 Rh6BG385400 Rh6DG377700
rosa_wichuraiana Rw6G032960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 435
AccII CGCG 1 cut(s) 609
AclWI GGATC 2 cut(s) 383, 468
AcsI RAATTY 2 cut(s) 53, 629
AcuI CTGAAG 1 cut(s) 574
AfaI GTAC 1 cut(s) 183
AfiI CCNNNNNNNGG 2 cut(s) 64, 65
AgsI TTSAA 3 cut(s) 241, 619, 635
AjnI CCWGG 1 cut(s) 133
AluBI AGCT 6 cut(s) 122, 291, 323, 373, 454, 538
AluI AGCT 6 cut(s) 122, 291, 323, 373, 454, 538
Alw21I GWGCWC 2 cut(s) 124, 525
Alw26I GTCTC 3 cut(s) 99, 224, 413
AlwI GGATC 2 cut(s) 383, 468
AoxI GGCC 1 cut(s) 221
ApeKI GCWGC 3 cut(s) 23, 127, 320
ApoI RAATTY 2 cut(s) 53, 629
AsuHPI GGTGA 2 cut(s) 149, 367
AxyI CCTNAGG 1 cut(s) 639
BanII GRGCYC 1 cut(s) 124
Bbv12I GWGCWC 2 cut(s) 124, 525
BbvI GCAGC 3 cut(s) 35, 139, 332
BccI CCATC 2 cut(s) 358, 542
BciT130I CCWGG 1 cut(s) 135
BcoDI GTCTC 3 cut(s) 99, 224, 413
BisI GCNGC 3 cut(s) 24, 128, 321
BlpI GCTNAGC 2 cut(s) 123, 287
BlsI GCNGC 3 cut(s) 25, 129, 322
Bme1390I CCNGG 1 cut(s) 135
BmrFI CCNGG 1 cut(s) 135
BmsI GCATC 1 cut(s) 562
BpmI CTGGAG 1 cut(s) 399
Bpu1102I GCTNAGC 2 cut(s) 123, 287
BsaJI CCNNGG 2 cut(s) 18, 218
Bsc4I CCNNNNNNNGG 2 cut(s) 64, 65
Bse1I ACTGG 1 cut(s) 224
Bse21I CCTNAGG 1 cut(s) 639
BseBI CCWGG 1 cut(s) 135
BseDI CCNNGG 2 cut(s) 18, 218
BseGI GGATG 2 cut(s) 577, 649
BseLI CCNNNNNNNGG 2 cut(s) 64, 65
BseMII CTCAG 2 cut(s) 83, 137
BseNI ACTGG 1 cut(s) 224
BseXI GCAGC 3 cut(s) 35, 139, 332
BsgI GTGCAG 1 cut(s) 495
Bsh1236I CGCG 1 cut(s) 609
BshFI GGCC 1 cut(s) 223
BsiHKAI GWGCWC 2 cut(s) 124, 525
BslI CCNNNNNNNGG 2 cut(s) 64, 65
BsmAI GTCTC 3 cut(s) 99, 224, 413
BsmI GAATGC 1 cut(s) 347
BsnI GGCC 1 cut(s) 223
Bsp1286I GDGCHC 2 cut(s) 124, 525
Bsp143I GATC 3 cut(s) 352, 388, 460
Bsp1720I GCTNAGC 2 cut(s) 123, 287
Bsp19I CCATGG 1 cut(s) 18
BspANI GGCC 1 cut(s) 223
BspCNI CTCAG 2 cut(s) 84, 136
BspFNI CGCG 1 cut(s) 609
BspPI GGATC 2 cut(s) 383, 468
BsrI ACTGG 1 cut(s) 224
BssECI CCNNGG 2 cut(s) 18, 218
BssMI GATC 3 cut(s) 352, 388, 460
BssT1I CCWWGG 2 cut(s) 18, 218
Bst2UI CCWGG 1 cut(s) 135
Bst4CI ACNGT 1 cut(s) 214
Bst6I CTCTTC 2 cut(s) 97, 242
BstDEI CTNAG 4 cut(s) 92, 123, 287, 639
BstDSI CCRYGG 1 cut(s) 18
BstF5I GGATG 2 cut(s) 577, 649
BstFNI CGCG 1 cut(s) 609
BstKTI GATC 3 cut(s) 355, 391, 463
BstMAI GTCTC 3 cut(s) 99, 224, 413
BstMBI GATC 3 cut(s) 352, 388, 460
BstMWI GCNNNNNNNGC 1 cut(s) 23
BstNI CCWGG 1 cut(s) 135
BstNSI RCATGY 1 cut(s) 308
BstSCI CCNGG 1 cut(s) 133
BstUI CGCG 1 cut(s) 609
BstV1I GCAGC 3 cut(s) 35, 139, 332
Bsu36I CCTNAGG 1 cut(s) 639
BsuRI GGCC 1 cut(s) 223
BtgI CCRYGG 1 cut(s) 18
BtsCI GGATG 2 cut(s) 577, 649
BtsIMutI CAGTG 2 cut(s) 219, 231
CseI GACGC 1 cut(s) 488
CsiI ACCWGGT 1 cut(s) 133
Csp6I GTAC 1 cut(s) 182
CspCI CAANNNNNGTGG 2 cut(s) 207, 242
CviAII CATG 5 cut(s) 19, 259, 305, 568, 595
CviQI GTAC 1 cut(s) 182
DdeI CTNAG 4 cut(s) 92, 123, 287, 639
DpnI GATC 3 cut(s) 354, 390, 462
DpnII GATC 3 cut(s) 352, 388, 460
Eam1104I CTCTTC 2 cut(s) 97, 242
EarI CTCTTC 2 cut(s) 97, 242
Ecl136II GAGCTC 1 cut(s) 122
Eco130I CCWWGG 2 cut(s) 18, 218
Eco24I GRGCYC 1 cut(s) 124
Eco53kI GAGCTC 1 cut(s) 122
Eco57I CTGAAG 1 cut(s) 574
Eco81I CCTNAGG 1 cut(s) 639
EcoICRI GAGCTC 1 cut(s) 122
EcoRII CCWGG 1 cut(s) 133
EcoT14I CCWWGG 2 cut(s) 18, 218
EcoT38I GRGCYC 1 cut(s) 124
ErhI CCWWGG 2 cut(s) 18, 218
FaeI CATG 5 cut(s) 22, 262, 308, 571, 598
FatI CATG 5 cut(s) 18, 258, 304, 567, 594
FblI GTMKAC 1 cut(s) 435
Fnu4HI GCNGC 3 cut(s) 24, 128, 321
FokI GGATG 1 cut(s) 584
FriOI GRGCYC 1 cut(s) 124
Fsp4HI GCNGC 3 cut(s) 24, 128, 321
GluI GCNGC 3 cut(s) 24, 128, 321
GsuI CTGGAG 1 cut(s) 399
HaeIII GGCC 1 cut(s) 223
HgaI GACGC 1 cut(s) 488
Hin1II CATG 5 cut(s) 22, 262, 308, 571, 598
HindIII AAGCTT 1 cut(s) 536
HinfI GANTC 3 cut(s) 84, 412, 579
HphI GGTGA 2 cut(s) 149, 367
Hpy166II GTNNAC 2 cut(s) 411, 436
Hpy188I TCNGA 3 cut(s) 357, 504, 514
Hpy188III TCNNGA 2 cut(s) 416, 641
Hpy8I GTNNAC 2 cut(s) 411, 436
HpyCH4III ACNGT 1 cut(s) 214
HpyCH4V TGCA 5 cut(s) 50, 320, 347, 476, 575
HpyF10VI GCNNNNNNNGC 1 cut(s) 23
HpyF3I CTNAG 4 cut(s) 92, 123, 287, 639
Hsp92II CATG 5 cut(s) 22, 262, 308, 571, 598
Kzo9I GATC 3 cut(s) 352, 388, 460
LpnPI CCDG 9 cut(s) 60, 120, 147, 189, 237, 348, 355, 429, 626
Lsp1109I GCAGC 3 cut(s) 35, 139, 332
LweI GCATC 1 cut(s) 562
MabI ACCWGGT 1 cut(s) 133
MalI GATC 3 cut(s) 354, 390, 462
MboI GATC 3 cut(s) 352, 388, 460
MboII GAAGA 5 cut(s) 24, 90, 93, 114, 259
MfeI CAATTG 1 cut(s) 236
MhlI GDGCHC 2 cut(s) 124, 525
MluCI AATT 3 cut(s) 53, 236, 629
MlyI GAGTC 2 cut(s) 406, 588
MmeI TCCRAC 2 cut(s) 112, 416
MnlI CCTC 3 cut(s) 463, 615, 648
MseI TTAA 1 cut(s) 585
MspR9I CCNGG 1 cut(s) 135
MunI CAATTG 1 cut(s) 236
Mva1269I GAATGC 1 cut(s) 347
MvaI CCWGG 1 cut(s) 135
MvnI CGCG 1 cut(s) 609
MwoI GCNNNNNNNGC 1 cut(s) 23
NcoI CCATGG 1 cut(s) 18
NdeII GATC 3 cut(s) 352, 388, 460
NlaIII CATG 5 cut(s) 22, 262, 308, 571, 598
NspI RCATGY 1 cut(s) 308
PctI GAATGC 1 cut(s) 347
PfeI GAWTC 1 cut(s) 84
PkrI GCNGC 3 cut(s) 25, 129, 322
PleI GAGTC 2 cut(s) 406, 587
PpsI GAGTC 2 cut(s) 406, 587
Psp124BI GAGCTC 1 cut(s) 124
Psp6I CCWGG 1 cut(s) 133
PspGI CCWGG 1 cut(s) 133
PsrI GAACNNNNNNTAC 4 cut(s) 150, 165, 182, 197
RsaI GTAC 1 cut(s) 183
RsaNI GTAC 1 cut(s) 182
SacI GAGCTC 1 cut(s) 124
SaqAI TTAA 1 cut(s) 585
SatI GCNGC 3 cut(s) 24, 128, 321
Sau3AI GATC 3 cut(s) 352, 388, 460
SchI GAGTC 2 cut(s) 406, 588
ScrFI CCNGG 1 cut(s) 135
SduI GDGCHC 2 cut(s) 124, 525
SexAI ACCWGGT 1 cut(s) 133
SfaNI GCATC 1 cut(s) 562
Sse9I AATT 3 cut(s) 53, 236, 629
SstI GAGCTC 1 cut(s) 124
StyD4I CCNGG 1 cut(s) 133
StyI CCWWGG 2 cut(s) 18, 218
TaaI ACNGT 1 cut(s) 214
TaqI TCGA 1 cut(s) 351
TasI AATT 3 cut(s) 53, 236, 629
TatI WGTACW 1 cut(s) 181
TfiI GAWTC 1 cut(s) 84
Tru1I TTAA 1 cut(s) 585
Tru9I TTAA 1 cut(s) 585
TscAI CASTG 2 cut(s) 219, 231
TseI GCWGC 3 cut(s) 23, 127, 320
TspRI CASTG 2 cut(s) 219, 231
XapI RAATTY 2 cut(s) 53, 629
XceI RCATGY 1 cut(s) 308
XmiI GTMKAC 1 cut(s) 435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.