FvH4_2g27960

NAD(P)H-quinone oxidoreductase subunit M

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0016132
Physical Location & Seq
Forward (+)
22083898 .. 22085718
1821 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g27960.t1

Sequence Viewer

Length: 651 bp
ATGCTACTACATTCAGGCATGGCAGCAACTTCTTCTTATATGGCTTGTGCCAAATTCTCTATGTTGGGTTATTCTGGAAGAAGAAAACAACTGAGAAGCAGAAGAGACTTCACCATAAGAGCTCAGCAGCAACCTGGTGAAGTCCAAGAACCACAAGAAATGAAAGTACAAGAACCAGAAGTAAAGAGAGGAAAAGGAGAGCCAAAAGGGACAGTGCCAAGGCCAGTGGAGGCACAATTGAATGTGCAGAGTAAGAACATGGGGAGAGAATATGGTGGGGATTGGCTTAGCAGTGCCACAAGGCATGTGAGGATTTATGCAGCTTATATAGACCCAGAAACCTGTGCATTTGATCAAACACAGATGGATAAGCTCACCATTATACTTGATCCAACCAATGAGTTTGTGTGGACTCCAGAGACTACCAACAAGGTCTACTCCTACTTCCAAGAGCTTGTGGATCACTATGAGGGTGCAGATTTGACAGAATACACTCTGCGTCTGATTGGTTCAGACATAGAGCACTACATAAGGAAGCTTCTGTATGATGGAGAAATCAAATACAACATGGATGCAAGAGTCCTTAACTTCAGCATGGGTAAGCCTCGTGTTATGTTCAATAACAACGAATTTCAACCTCAGGATGCATGA

Protein Analysis

217

Amino Acids

24.93

Weight (kDa)

6.13

Isoelectric Point (pI)

49.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NdhM PF10664 96 - 204 3.7e-46 Cyanobacterial and plastid NDH-1 subunit M
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016132)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G37925
fragaria_vesca FvH4_2g27960
malus_domestica MD15G1133300.v1.1
prunus_persica Prupe.1G488900_v2.0.a1
pyrus_communis pycom15g12020
rosa_chinensis RchiOBHm_Chr6g0296781
rosa_laevigata RLG00000011659
rosa_multiflora Rmu_ssc0000192.1_g000012
rosa_roxburghii Rroxscaffold_7G00171170
rosa_rugosa Rorug06G0266100 Rorug06G0266200
rosa_samantha Rh6AG377200 Rh6BG385400 Rh6DG377700
rosa_wichuraiana Rw6G032960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 435
AclWI GGATC 2 cut(s) 383, 468
AcsI RAATTY 2 cut(s) 53, 629
AcuI CTGAAG 1 cut(s) 574
AfaI GTAC 1 cut(s) 168
AgsI TTSAA 3 cut(s) 241, 619, 635
AjnI CCWGG 1 cut(s) 133
AluBI AGCT 5 cut(s) 122, 323, 373, 454, 538
AluI AGCT 5 cut(s) 122, 323, 373, 454, 538
Alw21I GWGCWC 2 cut(s) 124, 525
Alw26I GTCTC 2 cut(s) 99, 413
AlwI GGATC 2 cut(s) 383, 468
AoxI GGCC 1 cut(s) 221
ApeKI GCWGC 3 cut(s) 23, 127, 320
ApoI RAATTY 2 cut(s) 53, 629
AsuHPI GGTGA 3 cut(s) 103, 149, 367
AxyI CCTNAGG 1 cut(s) 639
BanII GRGCYC 1 cut(s) 124
BauI CACGAG 1 cut(s) 606
Bbv12I GWGCWC 2 cut(s) 124, 525
BbvI GCAGC 3 cut(s) 35, 139, 332
BccI CCATC 2 cut(s) 358, 542
BciT130I CCWGG 1 cut(s) 135
BclI TGATCA 1 cut(s) 352
BcoDI GTCTC 2 cut(s) 99, 413
BisI GCNGC 3 cut(s) 24, 128, 321
BlpI GCTNAGC 2 cut(s) 123, 287
BlsI GCNGC 3 cut(s) 25, 129, 322
Bme1390I CCNGG 1 cut(s) 135
BmrFI CCNGG 1 cut(s) 135
BmsI GCATC 2 cut(s) 562, 634
BpmI CTGGAG 1 cut(s) 399
Bpu1102I GCTNAGC 2 cut(s) 123, 287
BsaJI CCNNGG 1 cut(s) 218
Bse1I ACTGG 1 cut(s) 224
Bse21I CCTNAGG 1 cut(s) 639
BseBI CCWGG 1 cut(s) 135
BseDI CCNNGG 1 cut(s) 218
BseGI GGATG 2 cut(s) 577, 649
BseMII CTCAG 2 cut(s) 83, 137
BseNI ACTGG 1 cut(s) 224
BseXI GCAGC 3 cut(s) 35, 139, 332
BsgI GTGCAG 2 cut(s) 266, 495
BshFI GGCC 1 cut(s) 223
BsiHKAI GWGCWC 2 cut(s) 124, 525
BslFI GGGAC 1 cut(s) 223
BsmAI GTCTC 2 cut(s) 99, 413
BsmFI GGGAC 1 cut(s) 223
BsnI GGCC 1 cut(s) 223
Bsp1286I GDGCHC 2 cut(s) 124, 525
Bsp143I GATC 3 cut(s) 352, 388, 460
Bsp1720I GCTNAGC 2 cut(s) 123, 287
BspANI GGCC 1 cut(s) 223
BspCNI CTCAG 2 cut(s) 84, 136
BspPI GGATC 2 cut(s) 383, 468
BsrI ACTGG 1 cut(s) 224
BssECI CCNNGG 1 cut(s) 218
BssMI GATC 3 cut(s) 352, 388, 460
BssSI CACGAG 1 cut(s) 606
BssT1I CCWWGG 1 cut(s) 218
Bst2BI CACGAG 1 cut(s) 606
Bst2UI CCWGG 1 cut(s) 135
Bst4CI ACNGT 1 cut(s) 214
Bst6I CTCTTC 1 cut(s) 97
BstDEI CTNAG 4 cut(s) 92, 123, 287, 639
BstF5I GGATG 2 cut(s) 577, 649
BstKTI GATC 3 cut(s) 355, 391, 463
BstMAI GTCTC 2 cut(s) 99, 413
BstMBI GATC 3 cut(s) 352, 388, 460
BstNI CCWGG 1 cut(s) 135
BstNSI RCATGY 1 cut(s) 308
BstSCI CCNGG 1 cut(s) 133
BstV1I GCAGC 3 cut(s) 35, 139, 332
Bsu36I CCTNAGG 1 cut(s) 639
BsuRI GGCC 1 cut(s) 223
BtsCI GGATG 2 cut(s) 577, 649
BtsI GCAGTG 1 cut(s) 298
BtsIMutI CAGTG 3 cut(s) 219, 231, 298
CseI GACGC 1 cut(s) 488
CsiI ACCWGGT 1 cut(s) 133
Csp6I GTAC 1 cut(s) 167
CspCI CAANNNNNGTGG 2 cut(s) 207, 242
CviAII CATG 6 cut(s) 19, 259, 305, 568, 595, 648
CviQI GTAC 1 cut(s) 167
DdeI CTNAG 4 cut(s) 92, 123, 287, 639
DpnI GATC 3 cut(s) 354, 390, 462
DpnII GATC 3 cut(s) 352, 388, 460
Eam1104I CTCTTC 1 cut(s) 97
EarI CTCTTC 1 cut(s) 97
Ecl136II GAGCTC 1 cut(s) 122
Eco130I CCWWGG 1 cut(s) 218
Eco24I GRGCYC 1 cut(s) 124
Eco53kI GAGCTC 1 cut(s) 122
Eco57I CTGAAG 1 cut(s) 574
Eco81I CCTNAGG 1 cut(s) 639
EcoICRI GAGCTC 1 cut(s) 122
EcoRII CCWGG 1 cut(s) 133
EcoT14I CCWWGG 1 cut(s) 218
EcoT22I ATGCAT 1 cut(s) 649
EcoT38I GRGCYC 1 cut(s) 124
ErhI CCWWGG 1 cut(s) 218
FaeI CATG 6 cut(s) 22, 262, 308, 571, 598, 651
FaqI GGGAC 1 cut(s) 223
FatI CATG 6 cut(s) 18, 258, 304, 567, 594, 647
FbaI TGATCA 1 cut(s) 352
FblI GTMKAC 1 cut(s) 435
Fnu4HI GCNGC 3 cut(s) 24, 128, 321
FokI GGATG 1 cut(s) 584
FriOI GRGCYC 1 cut(s) 124
Fsp4HI GCNGC 3 cut(s) 24, 128, 321
GluI GCNGC 3 cut(s) 24, 128, 321
GsuI CTGGAG 1 cut(s) 399
HaeIII GGCC 1 cut(s) 223
HgaI GACGC 1 cut(s) 488
Hin1II CATG 6 cut(s) 22, 262, 308, 571, 598, 651
HindIII AAGCTT 1 cut(s) 536
HinfI GANTC 2 cut(s) 412, 579
HphI GGTGA 3 cut(s) 103, 149, 367
Hpy166II GTNNAC 2 cut(s) 411, 436
Hpy188I TCNGA 2 cut(s) 504, 514
Hpy188III TCNNGA 3 cut(s) 75, 416, 641
Hpy8I GTNNAC 2 cut(s) 411, 436
HpyCH4III ACNGT 1 cut(s) 214
HpyCH4V TGCA 6 cut(s) 247, 320, 347, 476, 575, 647
HpyF3I CTNAG 4 cut(s) 92, 123, 287, 639
Hsp92II CATG 6 cut(s) 22, 262, 308, 571, 598, 651
Ksp22I TGATCA 1 cut(s) 352
Kzo9I GATC 3 cut(s) 352, 388, 460
LpnPI CCDG 9 cut(s) 60, 120, 147, 189, 237, 348, 355, 429, 626
Lsp1109I GCAGC 3 cut(s) 35, 139, 332
LweI GCATC 2 cut(s) 562, 634
MabI ACCWGGT 1 cut(s) 133
MalI GATC 3 cut(s) 354, 390, 462
MboI GATC 3 cut(s) 352, 388, 460
MboII GAAGA 4 cut(s) 24, 90, 93, 114
MfeI CAATTG 1 cut(s) 236
MhlI GDGCHC 2 cut(s) 124, 525
MluCI AATT 3 cut(s) 53, 236, 629
MlyI GAGTC 2 cut(s) 406, 588
MmeI TCCRAC 1 cut(s) 416
MnlI CCTC 6 cut(s) 182, 223, 303, 463, 615, 648
Mph1103I ATGCAT 1 cut(s) 649
MseI TTAA 1 cut(s) 585
MspR9I CCNGG 1 cut(s) 135
MunI CAATTG 1 cut(s) 236
MvaI CCWGG 1 cut(s) 135
NdeII GATC 3 cut(s) 352, 388, 460
NlaIII CATG 6 cut(s) 22, 262, 308, 571, 598, 651
NsiI ATGCAT 1 cut(s) 649
NspI RCATGY 1 cut(s) 308
PkrI GCNGC 3 cut(s) 25, 129, 322
PleI GAGTC 2 cut(s) 406, 587
PpsI GAGTC 2 cut(s) 406, 587
Psp124BI GAGCTC 1 cut(s) 124
Psp6I CCWGG 1 cut(s) 133
PspGI CCWGG 1 cut(s) 133
RsaI GTAC 1 cut(s) 168
RsaNI GTAC 1 cut(s) 167
SacI GAGCTC 1 cut(s) 124
SaqAI TTAA 1 cut(s) 585
SatI GCNGC 3 cut(s) 24, 128, 321
Sau3AI GATC 3 cut(s) 352, 388, 460
SchI GAGTC 2 cut(s) 406, 588
ScrFI CCNGG 1 cut(s) 135
SduI GDGCHC 2 cut(s) 124, 525
SetI ASST 9 cut(s) 124, 136, 325, 344, 375, 435, 456, 540, 640
SexAI ACCWGGT 1 cut(s) 133
SfaNI GCATC 2 cut(s) 562, 634
Sse9I AATT 3 cut(s) 53, 236, 629
SstI GAGCTC 1 cut(s) 124
StyD4I CCNGG 1 cut(s) 133
StyI CCWWGG 1 cut(s) 218
TaaI ACNGT 1 cut(s) 214
TasI AATT 3 cut(s) 53, 236, 629
TatI WGTACW 1 cut(s) 166
Tru1I TTAA 1 cut(s) 585
Tru9I TTAA 1 cut(s) 585
TscAI CASTG 3 cut(s) 219, 231, 298
TseI GCWGC 3 cut(s) 23, 127, 320
TspDTI ATGAA 1 cut(s) 176
TspRI CASTG 3 cut(s) 219, 231, 298
XapI RAATTY 2 cut(s) 53, 629
XceI RCATGY 1 cut(s) 308
XmiI GTMKAC 1 cut(s) 435
Zsp2I ATGCAT 1 cut(s) 649
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.