AT4G38500

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0006752
Physical Location & Seq
Forward (+)
18007756 .. 18010903
3148 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G38500.1

Sequence Viewer

Length: 1500 bp
ATGATGAGTGACTCTCAGCGATCACTTTCTGTGCGATTGAGCCGCCGGGGTGAGAGAAGCAATCATTACTCACAATCTAAAGATGCTGTGGCTGGCTTCTTCTCTCCCACCAGATTGCATGCTGATTACACTTTGAAAAAAATTTGGAAGGCAGGCTTCCTTCGCTTGCTTCTTGTTGGCGGCATTGTCTGGATGCTACTCATTCTATTTGCCCTCTTGTTTCATGTTTGGTCCTGTCAATCTTCCCTTTCTTTCTTCTCAGCTATTTGCAACAAAGAAGGCAGACTCTATGTCATGTTGGACACTATTGGATTTGTACCTAAACCCCAGCACCGATGCCCCATTCCCGTTGATTATGACCCTGATAAGGTGCTCCTACCAAGTGATAAAACCGCAGATACAATTGTCAGAAATTTGACCTACGTAACAGAGGATGAATCCTCTAAGTCTCAGTTTCCTCTATTTGGCGGAAACATAAGCTGGTCTGAACGAGAAGAAAGCTTCAAACTGAAACCTGAGATGAAGGTCCATTGCGGATTCATGCCACGTGGTGGTGCTGAAATGTCATCTCTGGATAAAGAATACGTAAAGAAGTGCAGATTTGTTGTTGCCACGGGGATTTTCGATGCATATGATGAACCCCACCAGCCATCAAACATAAGCAAGCGTTCTATGAATCTCTTCTGTTTCCTTATGGTGGTAGATGAGGTCTCTCTCGATTTCTTAAGAAAAAATACTACTGTGAGGAAAGATGTTGAAGGAGGCATATGGGTTGGCATTTGGCGTCTGATTCTACTGAAAACTCCTCCATATGATGAGCCTAGGAGGAACGGTAAAGTCCCGAAAATCTTAACTCACAGGTTATTCCCTGAAGCACAGTACAGCATTTGGATTGATGGGAAAATGGAGCTTATTGTTGACCCCTTGCTTATTCTAGAAAGGTATTTGTGGCGTGGGAAACATACTTTTGCCATTGCCCAACACAAACACCACCGAAACATATATGAGGAAGCTGATGCGTGCAAGAGGAGAAAGCGATACGCCCGACCTCTGGTTGATCTTCATATGAAAATATATCGATACGAGGGCTTGGAACCATGGAGCATCAAGAAGAACACTGTTAGTGATGTTCCAGAGGGAGCTGTGATAATAAGAGAGCACACTGCAATGAACAATCTGTTCAGCTGCCTGTGGTTCAATGAGGTACACCTGTTAACACCGAGGGACCAGCTAAGCTTTGGGTATGTAGTGGACAGACTAAAAGGAGCCTTCAAGGTGTTCATGTTCCAGAACTGTGAGTATAACTCTCTCTTCGAACTGCACCCTCACATTCGAGAGCATTCCTCGAAGATTGAGTGGGTCAAGAGTCTTCAGGAACTCAAAGGGAAGGGAGAGAGTTTGAAGGAAAGCCGAGGCGGCTTTGGGCTCTGGACTCCTTACCCTGGTGACCTTGATTCTGTTGAGCTGCCAAAAGTAGTAAGAACTTCTAAAGCTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616

Protein Analysis

499

Amino Acids

57.76

Weight (kDa)

9.2

Isoelectric Point (pI)

41.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOD1_MUCI70 PF04765 139 - 443 3.2e-129 TOD1/MUCI70, glycosyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 551
AciI CCGC 6 cut(s) 43, 180, 393, 468, 534, 1416
AcsI RAATTY 2 cut(s) 141, 412
AcuI CTGAAG 2 cut(s) 891, 1355
AcvI CACGTG 1 cut(s) 548
AcyI GRCGYC 1 cut(s) 784
AdeI CACNNNGTG 1 cut(s) 551
AfaI GTAC 3 cut(s) 318, 881, 1206
AfiI CCNNNNNNNGG 6 cut(s) 367, 464, 551, 697, 1051, 1442
AflII CTTAAG 1 cut(s) 724
AgsI TTSAA 6 cut(s) 136, 505, 758, 1198, 1273, 1402
AhdI GACNNNNNGTC 1 cut(s) 290
AjnI CCWGG 1 cut(s) 1441
Alw21I GWGCWC 2 cut(s) 375, 1161
Alw26I GTCTC 2 cut(s) 453, 715
ApeKI GCWGC 2 cut(s) 1185, 1465
ApoI RAATTY 2 cut(s) 141, 412
Asp700I GAANNNNTTC 1 cut(s) 680
AspA2I CCTAGG 1 cut(s) 821
AspS9I GGNCC 3 cut(s) 231, 526, 1225
AsuC2I CCSGG 1 cut(s) 47
AsuHPI GGTGA 2 cut(s) 62, 1457
AsuII TTCGAA 1 cut(s) 1314
AvaII GGWCC 3 cut(s) 231, 526, 1225
AvrII CCTAGG 1 cut(s) 821
BaeI ACNNNNGTAYC 4 cut(s) 390, 423, 1196, 1229
BanII GRGCYC 1 cut(s) 1428
BbrPI CACGTG 1 cut(s) 548
BbsI GAAGAC 1 cut(s) 1361
Bbv12I GWGCWC 2 cut(s) 375, 1161
BbvI GCAGC 2 cut(s) 1172, 1452
BccI CCATC 2 cut(s) 658, 890
BciT130I CCWGG 1 cut(s) 1443
BcnI CCSGG 1 cut(s) 47
BcoDI GTCTC 2 cut(s) 453, 715
BfaI CTAG 2 cut(s) 822, 935
BfrI CTTAAG 1 cut(s) 724
BglI GCCNNNNNGGC 1 cut(s) 1416
BisI GCNGC 5 cut(s) 43, 181, 1186, 1417, 1466
BlnI CCTAGG 1 cut(s) 821
BlpI GCTNAGC 1 cut(s) 1232
BlsI GCNGC 5 cut(s) 44, 182, 1187, 1418, 1467
Bme1390I CCNGG 2 cut(s) 47, 1443
Bme18I GGWCC 3 cut(s) 231, 526, 1225
BmeRI GACNNNNNGTC 1 cut(s) 290
BmgT120I GGNCC 3 cut(s) 231, 526, 1225
BmiI GGNNCC 3 cut(s) 1095, 1226, 1267
BmrFI CCNGG 2 cut(s) 47, 1443
BmsI GCATC 6 cut(s) 73, 183, 326, 616, 1006, 1113
BpiI GAAGAC 1 cut(s) 1361
BplI GAGNNNNNCTC 5 cut(s) 30, 1289, 1321, 1328, 1360
Bpu1102I GCTNAGC 1 cut(s) 1232
Bpu14I TTCGAA 1 cut(s) 1314
BpuMI CCSGG 1 cut(s) 47
Bsa29I ATCGAT 1 cut(s) 1078
BsaAI YACGTR 3 cut(s) 424, 548, 586
BsaHI GRCGYC 1 cut(s) 784
BsaI GGTCTC 1 cut(s) 715
BsaJI CCNNGG 7 cut(s) 46, 612, 821, 1097, 1220, 1411, 1441
BsaXI ACNNNNNCTCC 2 cut(s) 817, 847
Bsc4I CCNNNNNNNGG 6 cut(s) 367, 464, 551, 697, 1051, 1442
Bse3DI GCAATG 3 cut(s) 529, 972, 1173
BseBI CCWGG 1 cut(s) 1443
BseCI ATCGAT 1 cut(s) 1078
BseDI CCNNGG 7 cut(s) 46, 612, 821, 1097, 1220, 1411, 1441
BseGI GGATG 2 cut(s) 198, 439
BseLI CCNNNNNNNGG 6 cut(s) 367, 464, 551, 697, 1051, 1442
BseMI GCAATG 3 cut(s) 529, 972, 1173
BseMII CTCAG 4 cut(s) 29, 273, 464, 507
BseRI GAGGAG 2 cut(s) 795, 1042
BseXI GCAGC 2 cut(s) 1172, 1452
BseYI CCCAGC 1 cut(s) 327
BsgI GTGCAG 2 cut(s) 616, 1304
BshVI ATCGAT 1 cut(s) 1078
BsiHKAI GWGCWC 2 cut(s) 375, 1161
BsiSI CCGG 1 cut(s) 46
BslFI GGGAC 2 cut(s) 824, 1238
BslI CCNNNNNNNGG 6 cut(s) 367, 464, 551, 697, 1051, 1442
BsmAI GTCTC 2 cut(s) 453, 715
BsmFI GGGAC 2 cut(s) 824, 1238
BsmI GAATGC 1 cut(s) 1339
Bso31I GGTCTC 1 cut(s) 715
Bsp119I TTCGAA 1 cut(s) 1314
Bsp1286I GDGCHC 3 cut(s) 375, 1161, 1428
Bsp143I GATC 2 cut(s) 20, 1057
Bsp1720I GCTNAGC 1 cut(s) 1232
Bsp19I CCATGG 1 cut(s) 1097
BspACI CCGC 6 cut(s) 43, 180, 393, 468, 534, 1416
BspCNI CTCAG 4 cut(s) 28, 272, 463, 508
BspDI ATCGAT 1 cut(s) 1078
BspLI GGNNCC 3 cut(s) 1095, 1226, 1267
BspT104I TTCGAA 1 cut(s) 1314
BspTI CTTAAG 1 cut(s) 724
BspTNI GGTCTC 1 cut(s) 715
BsrDI GCAATG 3 cut(s) 529, 972, 1173
BssECI CCNNGG 7 cut(s) 46, 612, 821, 1097, 1220, 1411, 1441
BssMI GATC 2 cut(s) 20, 1057
BssNI GRCGYC 1 cut(s) 784
BssT1I CCWWGG 2 cut(s) 821, 1097
Bst2UI CCWGG 1 cut(s) 1443
Bst4CI ACNGT 5 cut(s) 742, 833, 879, 1120, 1295
Bst6I CTCTTC 2 cut(s) 686, 1316
BstACI GRCGYC 1 cut(s) 784
BstAFI CTTAAG 1 cut(s) 724
BstBAI YACGTR 3 cut(s) 424, 548, 586
BstBI TTCGAA 1 cut(s) 1314
BstC8I GCNNGC 6 cut(s) 94, 120, 154, 167, 665, 1021
BstDEI CTNAG 6 cut(s) 15, 259, 444, 450, 516, 1232
BstDSI CCRYGG 2 cut(s) 612, 1097
BstEII GGTNACC 1 cut(s) 1445
BstF5I GGATG 2 cut(s) 198, 439
BstKTI GATC 2 cut(s) 23, 1060
BstMAI GTCTC 2 cut(s) 453, 715
BstMBI GATC 2 cut(s) 20, 1057
BstMWI GCNNNNNNNGC 2 cut(s) 162, 1416
BstNI CCWGG 1 cut(s) 1443
BstNSI RCATGY 1 cut(s) 122
BstPI GGTNACC 1 cut(s) 1445
BstSCI CCNGG 2 cut(s) 45, 1441
BstSNI TACGTA 2 cut(s) 424, 586
BstV1I GCAGC 2 cut(s) 1172, 1452
BstV2I GAAGAC 1 cut(s) 1361
Bsu15I ATCGAT 1 cut(s) 1078
BsuTUI ATCGAT 1 cut(s) 1078
BtgI CCRYGG 2 cut(s) 612, 1097
BtsCI GGATG 2 cut(s) 198, 439
BtsI GCAGTG 1 cut(s) 1161
BtsIMutI CAGTG 2 cut(s) 1116, 1161
Cac8I GCNNGC 6 cut(s) 94, 120, 154, 167, 665, 1021
Cfr13I GGNCC 3 cut(s) 231, 526, 1225
ClaI ATCGAT 1 cut(s) 1078
CseI GACGC 1 cut(s) 773
Csp6I GTAC 3 cut(s) 317, 880, 1205
CviAII CATG 6 cut(s) 119, 224, 295, 541, 1098, 1282
CviQI GTAC 3 cut(s) 317, 880, 1205
DdeI CTNAG 6 cut(s) 15, 259, 444, 450, 516, 1232
DpnI GATC 2 cut(s) 22, 1059
DpnII GATC 2 cut(s) 20, 1057
DraIII CACNNNGTG 1 cut(s) 551
DriI GACNNNNNGTC 1 cut(s) 290
Eam1104I CTCTTC 2 cut(s) 686, 1316
Eam1105I GACNNNNNGTC 1 cut(s) 290
EarI CTCTTC 2 cut(s) 686, 1316
EciI GGCGGA 1 cut(s) 483
Eco105I TACGTA 2 cut(s) 424, 586
Eco130I CCWWGG 2 cut(s) 821, 1097
Eco24I GRGCYC 1 cut(s) 1428
Eco31I GGTCTC 1 cut(s) 715
Eco47I GGWCC 3 cut(s) 231, 526, 1225
Eco57I CTGAAG 2 cut(s) 891, 1355
Eco72I CACGTG 1 cut(s) 548
Eco91I GGTNACC 1 cut(s) 1445
EcoO65I GGTNACC 1 cut(s) 1445
EcoRII CCWGG 1 cut(s) 1441
EcoT14I CCWWGG 2 cut(s) 821, 1097
EcoT22I ATGCAT 1 cut(s) 631
EcoT38I GRGCYC 1 cut(s) 1428
ErhI CCWWGG 2 cut(s) 821, 1097
FaeI CATG 6 cut(s) 122, 227, 298, 544, 1101, 1285
FalI AAGNNNNNCTT 2 cut(s) 140, 172
FaqI GGGAC 2 cut(s) 824, 1238
FatI CATG 6 cut(s) 118, 223, 294, 540, 1097, 1281
FauNDI CATATG 4 cut(s) 631, 767, 811, 1065
Fnu4HI GCNGC 5 cut(s) 43, 181, 1186, 1417, 1466
FokI GGATG 2 cut(s) 205, 446
FriOI GRGCYC 1 cut(s) 1428
Fsp4HI GCNGC 5 cut(s) 43, 181, 1186, 1417, 1466
FspBI CTAG 2 cut(s) 822, 935
GluI GCNGC 5 cut(s) 43, 181, 1186, 1417, 1466
GsaI CCCAGC 1 cut(s) 331
HapII CCGG 1 cut(s) 46
HgaI GACGC 1 cut(s) 773
Hin1I GRCGYC 1 cut(s) 784
Hin1II CATG 6 cut(s) 122, 227, 298, 544, 1101, 1285
HincII GTYRAC 2 cut(s) 919, 1215
HindII GTYRAC 2 cut(s) 919, 1215
HindIII AAGCTT 2 cut(s) 499, 1234
HinfI GANTC 9 cut(s) 11, 285, 437, 537, 676, 790, 1366, 1432, 1454
HpaI GTTAAC 1 cut(s) 1215
HpaII CCGG 1 cut(s) 46
HphI GGTGA 2 cut(s) 62, 1457
Hpy166II GTNNAC 4 cut(s) 919, 1207, 1215, 1252
Hpy188I TCNGA 3 cut(s) 410, 487, 789
Hpy8I GTNNAC 4 cut(s) 919, 1207, 1215, 1252
HpyAV CCTTC 8 cut(s) 142, 170, 272, 517, 752, 1279, 1381, 1396
HpyCH4III ACNGT 5 cut(s) 742, 833, 879, 1120, 1295
HpyCH4IV ACGT 3 cut(s) 423, 547, 585
HpyCH4V TGCA 7 cut(s) 118, 270, 597, 629, 1023, 1166, 1321
HpyF10VI GCNNNNNNNGC 2 cut(s) 162, 1416
HpyF3I CTNAG 6 cut(s) 15, 259, 444, 450, 516, 1232
HpySE526I ACGT 3 cut(s) 423, 547, 585
Hsp92I GRCGYC 1 cut(s) 784
Hsp92II CATG 6 cut(s) 122, 227, 298, 544, 1101, 1285
KspAI GTTAAC 1 cut(s) 1215
Kzo9I GATC 2 cut(s) 20, 1057
LmnI GCTCC 5 cut(s) 378, 907, 1101, 1139, 1265
Lsp1109I GCAGC 2 cut(s) 1172, 1452
LweI GCATC 6 cut(s) 73, 183, 326, 616, 1006, 1113
MaeI CTAG 2 cut(s) 822, 935
MaeII ACGT 3 cut(s) 423, 547, 585
MaeIII GTNAC 3 cut(s) 8, 424, 1445
MalI GATC 2 cut(s) 22, 1059
MboI GATC 2 cut(s) 20, 1057
MfeI CAATTG 1 cut(s) 402
MhlI GDGCHC 3 cut(s) 375, 1161, 1428
MluCI AATT 3 cut(s) 141, 402, 412
MlyI GAGTC 4 cut(s) 5, 279, 1375, 1426
MmeI TCCRAC 1 cut(s) 279
Mph1103I ATGCAT 1 cut(s) 631
MroXI GAANNNNTTC 1 cut(s) 680
MseI TTAA 3 cut(s) 725, 851, 1214
MslI CAYNNNNRTG 1 cut(s) 1166
MspA1I CMGCKG 1 cut(s) 1185
MspCI CTTAAG 1 cut(s) 724
MspI CCGG 1 cut(s) 46
MspR9I CCNGG 2 cut(s) 47, 1443
MunI CAATTG 1 cut(s) 402
Mva1269I GAATGC 1 cut(s) 1339
MvaI CCWGG 1 cut(s) 1443
MwoI GCNNNNNNNGC 2 cut(s) 162, 1416
NciI CCSGG 1 cut(s) 47
NcoI CCATGG 1 cut(s) 1097
NdeI CATATG 4 cut(s) 631, 767, 811, 1065
NdeII GATC 2 cut(s) 20, 1057
NlaIII CATG 6 cut(s) 122, 227, 298, 544, 1101, 1285
NlaIV GGNNCC 3 cut(s) 1095, 1226, 1267
NmeAIII GCCGAG 1 cut(s) 1436
NmuCI GTSAC 2 cut(s) 8, 1445
NsiI ATGCAT 1 cut(s) 631
NspI RCATGY 1 cut(s) 122
NspV TTCGAA 1 cut(s) 1314
PaeI GCATGC 1 cut(s) 122
PctI GAATGC 1 cut(s) 1339
PdmI GAANNNNTTC 1 cut(s) 680
PfeI GAWTC 5 cut(s) 437, 537, 676, 790, 1454
PflMI CCANNNNNTGG 1 cut(s) 551
PkrI GCNGC 5 cut(s) 44, 182, 1187, 1418, 1467
PleI GAGTC 4 cut(s) 5, 279, 1374, 1426
PmaCI CACGTG 1 cut(s) 548
PmlI CACGTG 1 cut(s) 548
PpsI GAGTC 4 cut(s) 5, 279, 1374, 1426
Ppu21I YACGTR 3 cut(s) 424, 548, 586
Psp6I CCWGG 1 cut(s) 1441
PspCI CACGTG 1 cut(s) 548
PspEI GGTNACC 1 cut(s) 1445
PspFI CCCAGC 1 cut(s) 327
PspGI CCWGG 1 cut(s) 1441
PspN4I GGNNCC 3 cut(s) 1095, 1226, 1267
PspPI GGNCC 3 cut(s) 231, 526, 1225
PvuII CAGCTG 1 cut(s) 1185
RsaI GTAC 3 cut(s) 318, 881, 1206
RsaNI GTAC 3 cut(s) 317, 880, 1205
RseI CAYNNNNRTG 1 cut(s) 1166
SaqAI TTAA 3 cut(s) 725, 851, 1214
SatI GCNGC 5 cut(s) 43, 181, 1186, 1417, 1466
Sau3AI GATC 2 cut(s) 20, 1057
Sau96I GGNCC 3 cut(s) 231, 526, 1225
SchI GAGTC 4 cut(s) 5, 279, 1375, 1426
ScrFI CCNGG 2 cut(s) 47, 1443
SduI GDGCHC 3 cut(s) 375, 1161, 1428
SfaNI GCATC 6 cut(s) 73, 183, 326, 616, 1006, 1113
SfuI TTCGAA 1 cut(s) 1314
SinI GGWCC 3 cut(s) 231, 526, 1225
SmiMI CAYNNNNRTG 1 cut(s) 1166
SmlI CTYRAG 1 cut(s) 724
SmoI CTYRAG 1 cut(s) 724
SnaBI TACGTA 2 cut(s) 424, 586
SphI GCATGC 1 cut(s) 122
Sse9I AATT 3 cut(s) 141, 402, 412
SsiI CCGC 6 cut(s) 43, 180, 393, 468, 534, 1416
SspMI CTAG 2 cut(s) 822, 935
StyD4I CCNGG 2 cut(s) 45, 1441
StyI CCWWGG 2 cut(s) 821, 1097
TaaI ACNGT 5 cut(s) 742, 833, 879, 1120, 1295
TaiI ACGT 3 cut(s) 426, 550, 588
TaqI TCGA 6 cut(s) 624, 717, 1078, 1314, 1333, 1346
TasI AATT 3 cut(s) 141, 402, 412
TatI WGTACW 1 cut(s) 879
TauI GCSGC 3 cut(s) 45, 183, 1419
TfiI GAWTC 5 cut(s) 437, 537, 676, 790, 1454
Tru1I TTAA 3 cut(s) 725, 851, 1214
Tru9I TTAA 3 cut(s) 725, 851, 1214
TscAI CASTG 2 cut(s) 1123, 1168
TseFI GTSAC 2 cut(s) 8, 1445
TseI GCWGC 2 cut(s) 1185, 1465
Tsp45I GTSAC 2 cut(s) 8, 1445
TspRI CASTG 2 cut(s) 1123, 1168
Van91I CCANNNNNTGG 1 cut(s) 551
Vha464I CTTAAG 1 cut(s) 724
VpaK11BI GGWCC 3 cut(s) 231, 526, 1225
XapI RAATTY 2 cut(s) 141, 412
XbaI TCTAGA 1 cut(s) 934
XceI RCATGY 1 cut(s) 122
XcmI CCANNNNNNNNNTGG 1 cut(s) 1235
XmaJI CCTAGG 1 cut(s) 821
XmnI GAANNNNTTC 1 cut(s) 680
XspI CTAG 2 cut(s) 822, 935
Zsp2I ATGCAT 1 cut(s) 631
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.