MD10G1127500.v1.1

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0006752
Physical Location & Seq
Reverse (-)
20786699 .. 20790812
4114 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1127500.v1.1.491

Sequence Viewer

Length: 1557 bp
ATGGATAAAGAGAGTCCGCGGTCTATTTCTTTTCGCACACATCGTAGAGGCGAAAGGAGTAATTTAAACCAACATAGTAAAGATGTTGAAGAGGGATTCCCTCTGGGAAGGGTGACACAGGATTATCCCATGAAGATTGTTTGGAAGAAGGGCTTTATTCGATTGGTTCTTGTAGGTGGCATTTTGTGGATGCTACTAATTCTCCTCGCGTCATTATTTCATGTTTGGTCCTGCCAGTCTTCTATCTCTTTCTTCTCAGCTATGTGTAACAAAGAGAGCAAGGTCTTCTCCATGTTAGACTCCCTTGGGTTTGTACCAAAAGGACAATCGCAACACCGCTGTCCGATTCCTGTTGTCAGTGATCCTGATAAGATAAAAATTCCAAAAGGAAGAACTCCTGATGAAATTGTCAAAAACTTAACATACGTTTTAGATGATGAGATTTTGCCTGACGGAACTCTGTCATCCCCACTATTCGGAGGACATCAAAATTGGGCTCAGAGAGAGGAGAGTTTTAGACTAAAATCTGACATGAAGTTGCACTGTGGTTTTATTCGAAACGGTGGTGCTGAAATGGCTCCTGCAGACATCAACTATGCCAAAAAATGTAGGTTTGTGGTTGCATCAGGCATTTTTGATGGTTATGATGTACCCCATCAACCTTCAAATTTAAGTCCTCGTTCTAAGAAGCTCTTCTGCTTTCTCATGGCGGTCGATGAAACATCTCTTGAATTTATCAAGGGAAATGTGACAGTCAGAGAGGATAATGATGGGGGAAAATGGGTTGGTATCTGGCGTCTTGTTTTGTTGAAGCATCCGCCTTACGATGAGCCAAGAAGGAACGGGAAGGTCCCCAAGATATTAACCCACCGCATGTTCCCTAACACCCAGTACAGCATTTGGATCGATGGTAAAATGGAGCTCATGGTTGATCCATTGCAAATTTTAGAAAGATATTTATGGCGTGGGAAGCATACATTTGCCATTTCTCAGCACAAGCACCACCGCAGTATATACGAGGAGGCTGATGCAAACAAGCGGAGGAAGCGATATGCTCGACCTCTTATCGATCTACACATGAAAATTTATCGTTATGAGGGTTTGGAGGCATGGGATCCAACCAAGAAAACCGTTAGTGATGTACCAGAGGGTGCCATTATCGTACGGGAACATACTGCGATGAGTAATTTATTTAGCTGCTTGTGGTTCAATGAGGTCCACCTCTTCACACCAAGAGACCAACTGAGTTTTGGTTATATTGTGTACCGGTTAGGGGGGTTGTTTAAGTTCTTTATGTTTCCGAATTGTGAGTACAATTCCCTGTTTGTGTTGCATCCACACATAAGGGAGCATTCTTCTCGTATAGAGTGGGTAACAAGTTGGAATCAGCTCAAGGGGAATGGATCTATTTCAAATAAGGGTAAAGATTGGGACCAAGCCAGTGCGAAAAATGGTTTGGTAGAGAGTAGGGGAGGCTTAGGCTTGTGGACTCCTTACCCTGCAAATCTTGATTCAGTGGTCCTACCCCCGGTAGCAAGAACATCAAAAGCTGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616

Protein Analysis

519

Amino Acids

59.47

Weight (kDa)

9.35

Isoelectric Point (pI)

38.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOD1_MUCI70 PF04765 140 - 447 2.6e-127 TOD1/MUCI70, glycosyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1151
AccII CGCG 2 cut(s) 19, 209
AciI CCGC 8 cut(s) 17, 19, 337, 710, 818, 871, 1006, 1039
AclWI GGATC 6 cut(s) 356, 911, 926, 1109, 1122, 1411
AcsI RAATTY 5 cut(s) 378, 667, 731, 942, 1083
AcyI GRCGYC 1 cut(s) 796
AfaI GTAC 7 cut(s) 315, 651, 893, 1143, 1164, 1265, 1313
AfiI CCNNNNNNNGG 3 cut(s) 476, 1273, 1528
AgeI ACCGGT 1 cut(s) 1266
AgsI TTSAA 6 cut(s) 89, 666, 731, 811, 1210, 1413
AjuI GAANNNNNNNTTGG 2 cut(s) 1439, 1471
AluBI AGCT 6 cut(s) 260, 691, 922, 1197, 1390, 1550
AluI AGCT 6 cut(s) 260, 691, 922, 1197, 1390, 1550
Alw21I GWGCWC 1 cut(s) 924
Alw26I GTCTC 1 cut(s) 1230
AlwI GGATC 6 cut(s) 356, 911, 926, 1109, 1122, 1411
ApeKI GCWGC 1 cut(s) 1197
ApoI RAATTY 5 cut(s) 378, 667, 731, 942, 1083
AsiGI ACCGGT 1 cut(s) 1266
Asp700I GAANNNNTTC 1 cut(s) 692
AspS9I GGNCC 5 cut(s) 228, 850, 1216, 1432, 1519
AsuC2I CCSGG 1 cut(s) 1529
AsuHPI GGTGA 1 cut(s) 124
AsuII TTCGAA 1 cut(s) 556
AvaII GGWCC 5 cut(s) 228, 850, 1216, 1432, 1519
BamHI GGATCC 1 cut(s) 1114
BanI GGYRCC 1 cut(s) 1151
BanII GRGCYC 2 cut(s) 499, 924
BbsI GAAGAC 2 cut(s) 231, 277
Bbv12I GWGCWC 1 cut(s) 924
BbvI GCAGC 1 cut(s) 1184
BccI CCATC 4 cut(s) 632, 663, 764, 902
BcgI CGANNNNNNTGC 4 cut(s) 886, 920, 1340, 1374
BcnI CCSGG 1 cut(s) 1529
BcoDI GTCTC 1 cut(s) 1230
BfmI CTRYAG 1 cut(s) 582
BisI GCNGC 1 cut(s) 1198
BlsI GCNGC 1 cut(s) 1199
Bme1390I CCNGG 1 cut(s) 1529
Bme18I GGWCC 5 cut(s) 228, 850, 1216, 1432, 1519
BmgT120I GGNCC 5 cut(s) 228, 850, 1216, 1432, 1519
BmiI GGNNCC 5 cut(s) 579, 852, 1116, 1153, 1433
BmrFI CCNGG 1 cut(s) 1529
BmrI ACTGGG 1 cut(s) 883
BmsI GCATC 5 cut(s) 180, 632, 823, 1018, 1342
BmuI ACTGGG 1 cut(s) 883
BpiI GAAGAC 2 cut(s) 231, 277
Bpu10I CCTNAGC 1 cut(s) 1477
Bpu14I TTCGAA 1 cut(s) 556
BpuEI CTTGAG 1 cut(s) 1376
BpuMI CCSGG 1 cut(s) 1529
Bsa29I ATCGAT 2 cut(s) 906, 1068
BsaHI GRCGYC 1 cut(s) 796
BsaI GGTCTC 1 cut(s) 1230
BsaJI CCNNGG 3 cut(s) 17, 304, 1527
BsaWI WCCGGW 1 cut(s) 1266
BsaXI ACNNNNNCTCC 2 cut(s) 186, 216
Bsc4I CCNNNNNNNGG 3 cut(s) 476, 1273, 1528
Bse118I RCCGGY 1 cut(s) 1266
Bse1I ACTGG 3 cut(s) 235, 889, 1440
Bse3DI GCAATG 1 cut(s) 935
BseCI ATCGAT 2 cut(s) 906, 1068
BseDI CCNNGG 3 cut(s) 17, 304, 1527
BseGI GGATG 4 cut(s) 195, 464, 814, 1333
BseLI CCNNNNNNNGG 3 cut(s) 476, 1273, 1528
BseMI GCAATG 1 cut(s) 935
BseMII CTCAG 4 cut(s) 270, 512, 1004, 1235
BseNI ACTGG 3 cut(s) 235, 889, 1440
BseRI GAGGAG 3 cut(s) 194, 521, 1034
BseXI GCAGC 1 cut(s) 1184
Bsh1236I CGCG 2 cut(s) 19, 209
Bsh1285I CGRYCG 1 cut(s) 714
BshNI GGYRCC 1 cut(s) 1151
BshTI ACCGGT 1 cut(s) 1266
BshVI ATCGAT 2 cut(s) 906, 1068
BsiEI CGRYCG 1 cut(s) 714
BsiHKAI GWGCWC 1 cut(s) 924
BsiSI CCGG 2 cut(s) 1267, 1529
BsiWI CGTACG 1 cut(s) 1162
BslFI GGGAC 2 cut(s) 836, 1445
BslI CCNNNNNNNGG 3 cut(s) 476, 1273, 1528
BsmAI GTCTC 1 cut(s) 1230
BsmFI GGGAC 2 cut(s) 836, 1445
BsmI GAATGC 1 cut(s) 1351
Bso31I GGTCTC 1 cut(s) 1230
Bsp119I TTCGAA 1 cut(s) 556
Bsp1286I GDGCHC 2 cut(s) 499, 924
Bsp143I GATC 6 cut(s) 361, 903, 931, 1069, 1114, 1403
BspACI CCGC 8 cut(s) 17, 19, 337, 710, 818, 871, 1006, 1039
BspCNI CTCAG 4 cut(s) 269, 511, 1003, 1236
BspDI ATCGAT 2 cut(s) 906, 1068
BspFNI CGCG 2 cut(s) 19, 209
BspLI GGNNCC 5 cut(s) 579, 852, 1116, 1153, 1433
BspMAI CTGCAG 1 cut(s) 586
BspPI GGATC 6 cut(s) 356, 911, 926, 1109, 1122, 1411
BspQI GCTCTTC 1 cut(s) 698
BspT104I TTCGAA 1 cut(s) 556
BspT107I GGYRCC 1 cut(s) 1151
BspTNI GGTCTC 1 cut(s) 1230
BsrDI GCAATG 1 cut(s) 935
BsrFI RCCGGY 1 cut(s) 1266
BsrI ACTGG 3 cut(s) 235, 889, 1440
BssAI RCCGGY 1 cut(s) 1266
BssECI CCNNGG 3 cut(s) 17, 304, 1527
BssMI GATC 6 cut(s) 361, 903, 931, 1069, 1114, 1403
BssNI GRCGYC 1 cut(s) 796
BssT1I CCWWGG 1 cut(s) 304
Bst4CI ACNGT 4 cut(s) 545, 563, 754, 1132
Bst6I CTCTTC 3 cut(s) 84, 698, 1229
BstACI GRCGYC 1 cut(s) 796
BstBI TTCGAA 1 cut(s) 556
BstC8I GCNNGC 1 cut(s) 1552
BstDEI CTNAG 6 cut(s) 256, 498, 684, 990, 1244, 1477
BstDSI CCRYGG 1 cut(s) 17
BstF5I GGATG 4 cut(s) 195, 464, 814, 1333
BstFNI CGCG 2 cut(s) 19, 209
BstKTI GATC 6 cut(s) 364, 906, 934, 1072, 1117, 1406
BstMAI GTCTC 1 cut(s) 1230
BstMBI GATC 6 cut(s) 361, 903, 931, 1069, 1114, 1403
BstMCI CGRYCG 1 cut(s) 714
BstMWI GCNNNNNNNGC 3 cut(s) 575, 970, 1045
BstNSI RCATGY 1 cut(s) 877
BstSCI CCNGG 1 cut(s) 1527
BstSFI CTRYAG 1 cut(s) 582
BstUI CGCG 2 cut(s) 19, 209
BstV1I GCAGC 1 cut(s) 1184
BstV2I GAAGAC 2 cut(s) 231, 277
BstX2I RGATCY 2 cut(s) 1114, 1403
BstYI RGATCY 2 cut(s) 1114, 1403
Bsu15I ATCGAT 2 cut(s) 906, 1068
BsuTUI ATCGAT 2 cut(s) 906, 1068
BtgI CCRYGG 1 cut(s) 17
BtgZI GCGATG 1 cut(s) 1193
BtsCI GGATG 4 cut(s) 195, 464, 814, 1333
BtsIMutI CAGTG 4 cut(s) 364, 541, 1447, 1521
Cac8I GCNNGC 1 cut(s) 1552
Cfr10I RCCGGY 1 cut(s) 1266
Cfr13I GGNCC 5 cut(s) 228, 850, 1216, 1432, 1519
Cfr42I CCGCGG 1 cut(s) 20
ClaI ATCGAT 2 cut(s) 906, 1068
CseI GACGC 2 cut(s) 198, 785
Csp6I GTAC 7 cut(s) 314, 650, 892, 1142, 1163, 1264, 1312
CspAI ACCGGT 1 cut(s) 1266
CviAII CATG 9 cut(s) 130, 221, 292, 532, 706, 874, 925, 1078, 1110
CviQI GTAC 7 cut(s) 314, 650, 892, 1142, 1163, 1264, 1312
DdeI CTNAG 6 cut(s) 256, 498, 684, 990, 1244, 1477
DpnI GATC 6 cut(s) 363, 905, 933, 1071, 1116, 1405
DpnII GATC 6 cut(s) 361, 903, 931, 1069, 1114, 1403
DraI TTTAAA 1 cut(s) 66
Eam1104I CTCTTC 3 cut(s) 84, 698, 1229
EarI CTCTTC 3 cut(s) 84, 698, 1229
EciI GGCGGA 1 cut(s) 807
Ecl136II GAGCTC 1 cut(s) 922
Eco130I CCWWGG 1 cut(s) 304
Eco24I GRGCYC 2 cut(s) 499, 924
Eco31I GGTCTC 1 cut(s) 1230
Eco47I GGWCC 5 cut(s) 228, 850, 1216, 1432, 1519
Eco53kI GAGCTC 1 cut(s) 922
EcoICRI GAGCTC 1 cut(s) 922
EcoO109I RGGNCCY 1 cut(s) 850
EcoT14I CCWWGG 1 cut(s) 304
EcoT38I GRGCYC 2 cut(s) 499, 924
ErhI CCWWGG 1 cut(s) 304
FaeI CATG 9 cut(s) 133, 224, 295, 535, 709, 877, 928, 1081, 1113
FalI AAGNNNNNCTT 4 cut(s) 137, 169, 677, 709
FaqI GGGAC 2 cut(s) 836, 1445
FatI CATG 9 cut(s) 129, 220, 291, 531, 705, 873, 924, 1077, 1109
Fnu4HI GCNGC 1 cut(s) 1198
FokI GGATG 4 cut(s) 202, 451, 801, 1320
FriOI GRGCYC 2 cut(s) 499, 924
Fsp4HI GCNGC 1 cut(s) 1198
GluI GCNGC 1 cut(s) 1198
HapII CCGG 2 cut(s) 1267, 1529
HgaI GACGC 2 cut(s) 198, 785
Hin1I GRCGYC 1 cut(s) 796
Hin1II CATG 9 cut(s) 133, 224, 295, 535, 709, 877, 928, 1081, 1113
HinfI GANTC 7 cut(s) 13, 96, 299, 346, 1384, 1489, 1511
HpaII CCGG 2 cut(s) 1267, 1529
HphI GGTGA 1 cut(s) 124
Hpy166II GTNNAC 3 cut(s) 1219, 1264, 1488
Hpy188I TCNGA 6 cut(s) 345, 479, 501, 529, 758, 1302
Hpy188III TCNNGA 4 cut(s) 365, 398, 728, 1508
Hpy8I GTNNAC 3 cut(s) 1219, 1264, 1488
HpyAV CCTTC 5 cut(s) 102, 142, 672, 831, 841
HpyCH4III ACNGT 4 cut(s) 545, 563, 754, 1132
HpyCH4IV ACGT 1 cut(s) 426
HpyCH4V TGCA 7 cut(s) 541, 584, 623, 940, 1031, 1333, 1502
HpyF10VI GCNNNNNNNGC 3 cut(s) 575, 970, 1045
HpyF3I CTNAG 6 cut(s) 256, 498, 684, 990, 1244, 1477
HpySE526I ACGT 1 cut(s) 426
Hsp92I GRCGYC 1 cut(s) 796
Hsp92II CATG 9 cut(s) 133, 224, 295, 535, 709, 877, 928, 1081, 1113
KspI CCGCGG 1 cut(s) 20
Kzo9I GATC 6 cut(s) 361, 903, 931, 1069, 1114, 1403
LguI GCTCTTC 1 cut(s) 698
LmnI GCTCC 3 cut(s) 583, 919, 1348
Lsp1109I GCAGC 1 cut(s) 1184
LweI GCATC 5 cut(s) 180, 632, 823, 1018, 1342
MaeII ACGT 1 cut(s) 426
MaeIII GTNAC 4 cut(s) 112, 266, 748, 1372
MalI GATC 6 cut(s) 363, 905, 933, 1071, 1116, 1405
MboI GATC 6 cut(s) 361, 903, 931, 1069, 1114, 1403
MflI RGATCY 2 cut(s) 1114, 1403
MhlI GDGCHC 2 cut(s) 499, 924
MlyI GAGTC 3 cut(s) 22, 293, 1483
MmeI TCCRAC 2 cut(s) 1142, 1361
MroXI GAANNNNTTC 1 cut(s) 692
MseI TTAA 5 cut(s) 65, 419, 671, 863, 1284
MspA1I CMGCKG 2 cut(s) 19, 339
MspI CCGG 2 cut(s) 1267, 1529
MspR9I CCNGG 1 cut(s) 1529
Mva1269I GAATGC 1 cut(s) 1351
MvnI CGCG 2 cut(s) 19, 209
MwoI GCNNNNNNNGC 3 cut(s) 575, 970, 1045
NciI CCSGG 1 cut(s) 1529
NdeII GATC 6 cut(s) 361, 903, 931, 1069, 1114, 1403
NlaIII CATG 9 cut(s) 133, 224, 295, 535, 709, 877, 928, 1081, 1113
NlaIV GGNNCC 5 cut(s) 579, 852, 1116, 1153, 1433
NmuCI GTSAC 2 cut(s) 112, 748
NspI RCATGY 1 cut(s) 877
NspV TTCGAA 1 cut(s) 556
PciSI GCTCTTC 1 cut(s) 698
PcsI WCGNNNNNNNCGW 1 cut(s) 40
PctI GAATGC 1 cut(s) 1351
PdmI GAANNNNTTC 1 cut(s) 692
PfeI GAWTC 4 cut(s) 96, 346, 1384, 1511
Pfl23II CGTACG 1 cut(s) 1162
PinAI ACCGGT 1 cut(s) 1266
PkrI GCNGC 1 cut(s) 1199
PleI GAGTC 3 cut(s) 21, 293, 1483
PpsI GAGTC 3 cut(s) 21, 293, 1483
PpuMI RGGWCCY 1 cut(s) 850
Psp124BI GAGCTC 1 cut(s) 924
Psp5II RGGWCCY 1 cut(s) 850
PspLI CGTACG 1 cut(s) 1162
PspN4I GGNNCC 5 cut(s) 579, 852, 1116, 1153, 1433
PspPI GGNCC 5 cut(s) 228, 850, 1216, 1432, 1519
PspPPI RGGWCCY 1 cut(s) 850
PstI CTGCAG 1 cut(s) 586
PsuI RGATCY 2 cut(s) 1114, 1403
RsaI GTAC 7 cut(s) 315, 651, 893, 1143, 1164, 1265, 1313
RsaNI GTAC 7 cut(s) 314, 650, 892, 1142, 1163, 1264, 1312
SacI GAGCTC 1 cut(s) 924
SacII CCGCGG 1 cut(s) 20
SapI GCTCTTC 1 cut(s) 698
SaqAI TTAA 5 cut(s) 65, 419, 671, 863, 1284
SatI GCNGC 1 cut(s) 1198
Sau3AI GATC 6 cut(s) 361, 903, 931, 1069, 1114, 1403
Sau96I GGNCC 5 cut(s) 228, 850, 1216, 1432, 1519
SchI GAGTC 3 cut(s) 22, 293, 1483
ScrFI CCNGG 1 cut(s) 1529
SduI GDGCHC 2 cut(s) 499, 924
SfaNI GCATC 5 cut(s) 180, 632, 823, 1018, 1342
SfcI CTRYAG 1 cut(s) 582
Sfr303I CCGCGG 1 cut(s) 20
SfuI TTCGAA 1 cut(s) 556
SgrBI CCGCGG 1 cut(s) 20
SinI GGWCC 5 cut(s) 228, 850, 1216, 1432, 1519
SmlI CTYRAG 1 cut(s) 1391
SmoI CTYRAG 1 cut(s) 1391
SsiI CCGC 8 cut(s) 17, 19, 337, 710, 818, 871, 1006, 1039
SstI GAGCTC 1 cut(s) 924
StyD4I CCNGG 1 cut(s) 1527
StyI CCWWGG 1 cut(s) 304
TaaI ACNGT 4 cut(s) 545, 563, 754, 1132
TaiI ACGT 1 cut(s) 429
TaqI TCGA 6 cut(s) 160, 556, 714, 906, 1057, 1068
TatI WGTACW 2 cut(s) 891, 1311
TfiI GAWTC 4 cut(s) 96, 346, 1384, 1511
Tru1I TTAA 5 cut(s) 65, 419, 671, 863, 1284
Tru9I TTAA 5 cut(s) 65, 419, 671, 863, 1284
TscAI CASTG 4 cut(s) 364, 548, 1447, 1521
TseFI GTSAC 2 cut(s) 112, 748
TseI GCWGC 1 cut(s) 1197
Tsp45I GTSAC 2 cut(s) 112, 748
TspDTI ATGAA 6 cut(s) 146, 209, 417, 548, 732, 1094
TspGWI ACGGA 1 cut(s) 468
TspRI CASTG 4 cut(s) 364, 548, 1447, 1521
VpaK11BI GGWCC 5 cut(s) 228, 850, 1216, 1432, 1519
XapI RAATTY 5 cut(s) 378, 667, 731, 942, 1083
XceI RCATGY 1 cut(s) 877
XcmI CCANNNNNNNNNTGG 1 cut(s) 1247
XmnI GAANNNNTTC 1 cut(s) 692
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.