Rh6DG227200

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0006752
Physical Location & Seq
Reverse (-)
40649062 .. 40652958
3897 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG227200.1

Sequence Viewer

Length: 1296 bp
ATGTGCAACAAAAATAGCACGGTATTGGACATGTTACGCAATATGGGATTTGTAGCAAAACCACAACTGCAACACCGATGTCCAATTCCCGTTGCCAATGATCCTGATAAGATAGTTATACCAGAGGGGAGAACTCCCGATGAAATCGTCAAAAAGCTAACCTATGTTATGGAGGATGAGGCGGTATCTGACAAAACTCAGTCATCCCCACTATTTGGAGGACATGAAAGCTGGTCACAAAGAAAAGAGAGTTTTAGACTAAAACCAACCATGAAGGTGCACTGTGGTTTCATTCGAAATGGTGGTGGTGACATGAGTCCTGCAGACATTGAATATGCTGAGAAATGTAGGTTTGTGGTTGCATCGGGCATTTTTGATGGTTATGATGTACCCCATCAACCTTCAAATTTGAGCCCCCGTTCTAAAAAGCTCTTCTGTTTTTTCATGGTGGTGGATGAGACATCTCTTGAATTTATAAGTAAAAATGTTACTGTCAGAGAGGATAATAATGGGGGGAAATGGGTTGGCATTTGGCGTCTGGTTTTACTGAAGCATCCACCTTATGATGAACCAAGAAGGAATGGGAAGGTCCCCAAGATCTTGACCCACCGTTTATTCCCTAAGGCGCAGTACAGCATTTGGATTGATGGTAAAATGGAGCTCATAGTTGATCCATTGCTTATTCTGGAAAGATATTTATGGCGTCAGAAGAATACATTTGCCATTTCTCAGCACAAACATCATCACAGTATATATGAGGAGGCAGATGCAAACAAGCGGAGGAAACGATATGCTCGACCTCTTATTGATCTTCACATGAAGATTTACCGATACGAGGGCATGGAGCCATGGAGTCCACAGAAGAAAACTATTAGTGATGTGCCAGAGGGAGCTATTATCATACGGGAGCACACTGCAATGAGTAACTTGTTTAGCTGCTTGTGGTTCAATGAGGTCGACCTCTTTACACCAAGGGACCAACTGAGTTTTGGTTATGTTCTGTACAGGTTGGGGGATTCGTTTAGGTTCTTTATGTTTCCAAACTGTGAGTACTATTCACTGTTTATTTTGCATCCACACATAAGGGAGCATTCCTCTCGCATAGAGTGGGTAACTAGTTGGAGTCAGCTTAAGGGTAACGGAACCATTAAGGATAAGGGTAAGGATTGGGACCAAGCTAATGCAAAAAATGGTTTGGTAGAAAGTAGGGGAGGCTTAGGGTTATGGACTCCTTATCCTGCAAATCTTGATTCAGTTATCCTGCCGCCTGTAGCAAGAACATCAAAAGCTGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616

Protein Analysis

431

Amino Acids

49.79

Weight (kDa)

9.09

Isoelectric Point (pI)

41.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOD1_MUCI70 PF04765 53 - 360 1.2e-132 TOD1/MUCI70, glycosyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 476
AccB7I CCANNNNNTGG 1 cut(s) 215
AccI GTMKAC 1 cut(s) 957
AciI CCGC 3 cut(s) 182, 778, 1265
AclWI GGATC 2 cut(s) 95, 665
AcsI RAATTY 2 cut(s) 406, 470
AcuI CTGAAG 1 cut(s) 569
AcyI GRCGYC 2 cut(s) 535, 703
AfaI GTAC 4 cut(s) 390, 632, 1004, 1052
AfiI CCNNNNNNNGG 2 cut(s) 215, 835
AflII CTTAAG 1 cut(s) 1130
AflIII ACRYGT 1 cut(s) 30
AgsI TTSAA 4 cut(s) 332, 405, 470, 949
AhlI ACTAGT 1 cut(s) 1115
AluBI AGCT 9 cut(s) 157, 231, 430, 661, 893, 936, 1129, 1178, 1289
AluI AGCT 9 cut(s) 157, 231, 430, 661, 893, 936, 1129, 1178, 1289
Alw21I GWGCWC 3 cut(s) 282, 663, 912
Alw26I GTCTC 1 cut(s) 452
Alw44I GTGCAC 1 cut(s) 278
AlwI GGATC 2 cut(s) 95, 665
ApaLI GTGCAC 1 cut(s) 278
ApeKI GCWGC 1 cut(s) 936
ApoI RAATTY 2 cut(s) 406, 470
AspLEI GCGC 1 cut(s) 628
AspS9I GGNCC 3 cut(s) 589, 976, 1171
AsuHPI GGTGA 1 cut(s) 320
AsuII TTCGAA 1 cut(s) 295
AvaII GGWCC 3 cut(s) 589, 976, 1171
AxyI CCTNAGG 1 cut(s) 621
BaeGI GKGCMC 1 cut(s) 282
BanII GRGCYC 2 cut(s) 416, 663
Bbv12I GWGCWC 3 cut(s) 282, 663, 912
BbvI GCAGC 1 cut(s) 923
BccI CCATC 3 cut(s) 371, 402, 641
BcgI CGANNNNNNTGC 2 cut(s) 1079, 1113
BcoDI GTCTC 1 cut(s) 452
BcuI ACTAGT 1 cut(s) 1115
BfaI CTAG 1 cut(s) 1116
BfmI CTRYAG 2 cut(s) 321, 1269
BfrI CTTAAG 1 cut(s) 1130
BglII AGATCT 1 cut(s) 597
BisI GCNGC 2 cut(s) 937, 1265
BlsI GCNGC 2 cut(s) 938, 1266
BmcAI AGTACT 1 cut(s) 1052
Bme18I GGWCC 3 cut(s) 589, 976, 1171
BmgT120I GGNCC 3 cut(s) 589, 976, 1171
BmiI GGNNCC 5 cut(s) 591, 846, 977, 1144, 1172
BmsI GCATC 4 cut(s) 371, 562, 757, 1081
BoxI GACNNNNGTC 1 cut(s) 315
BplI GAGNNNNNCTC 2 cut(s) 1079, 1111
Bpu10I CCTNAGC 1 cut(s) 1216
Bpu14I TTCGAA 1 cut(s) 295
BsaHI GRCGYC 2 cut(s) 535, 703
BsaJI CCNNGG 2 cut(s) 848, 971
Bsc4I CCNNNNNNNGG 2 cut(s) 215, 835
Bse21I CCTNAGG 1 cut(s) 621
Bse3DI GCAATG 2 cut(s) 674, 924
BseDI CCNNGG 2 cut(s) 848, 971
BseGI GGATG 5 cut(s) 181, 203, 460, 553, 1072
BseLI CCNNNNNNNGG 2 cut(s) 215, 835
BseMI GCAATG 2 cut(s) 674, 924
BseMII CTCAG 4 cut(s) 212, 330, 743, 974
BseRI GAGGAG 1 cut(s) 773
BseSI GKGCMC 1 cut(s) 282
BseXI GCAGC 1 cut(s) 923
BsiHKAI GWGCWC 3 cut(s) 282, 663, 912
BslFI GGGAC 3 cut(s) 575, 989, 1184
BslI CCNNNNNNNGG 2 cut(s) 215, 835
BsmAI GTCTC 1 cut(s) 452
BsmFI GGGAC 3 cut(s) 575, 989, 1184
BsmI GAATGC 1 cut(s) 1090
Bsp119I TTCGAA 1 cut(s) 295
Bsp1286I GDGCHC 4 cut(s) 282, 416, 663, 912
Bsp1407I TGTACA 1 cut(s) 1002
Bsp143I GATC 4 cut(s) 100, 597, 670, 808
Bsp19I CCATGG 1 cut(s) 848
BspACI CCGC 3 cut(s) 182, 778, 1265
BspCNI CTCAG 4 cut(s) 211, 331, 742, 975
BspLI GGNNCC 5 cut(s) 591, 846, 977, 1144, 1172
BspMAI CTGCAG 1 cut(s) 325
BspPI GGATC 2 cut(s) 95, 665
BspQI GCTCTTC 1 cut(s) 437
BspT104I TTCGAA 1 cut(s) 295
BspTI CTTAAG 1 cut(s) 1130
BsrDI GCAATG 2 cut(s) 674, 924
BsrGI TGTACA 1 cut(s) 1002
BssECI CCNNGG 2 cut(s) 848, 971
BssMI GATC 4 cut(s) 100, 597, 670, 808
BssNI GRCGYC 2 cut(s) 535, 703
BssT1I CCWWGG 2 cut(s) 848, 971
Bst4CI ACNGT 7 cut(s) 22, 284, 493, 611, 749, 1046, 1062
Bst6I CTCTTC 1 cut(s) 437
BstACI GRCGYC 2 cut(s) 535, 703
BstAFI CTTAAG 1 cut(s) 1130
BstAUI TGTACA 1 cut(s) 1002
BstBI TTCGAA 1 cut(s) 295
BstC8I GCNNGC 1 cut(s) 1291
BstDEI CTNAG 6 cut(s) 198, 339, 621, 729, 983, 1216
BstDSI CCRYGG 1 cut(s) 848
BstF5I GGATG 5 cut(s) 181, 203, 460, 553, 1072
BstHHI GCGC 1 cut(s) 628
BstKTI GATC 4 cut(s) 103, 600, 673, 811
BstMAI GTCTC 1 cut(s) 452
BstMBI GATC 4 cut(s) 100, 597, 670, 808
BstNSI RCATGY 1 cut(s) 34
BstPAI GACNNNNGTC 1 cut(s) 315
BstSFI CTRYAG 2 cut(s) 321, 1269
BstSLI GKGCMC 1 cut(s) 282
BstV1I GCAGC 1 cut(s) 923
BstX2I RGATCY 1 cut(s) 597
BstYI RGATCY 1 cut(s) 597
Bsu36I CCTNAGG 1 cut(s) 621
BtgI CCRYGG 1 cut(s) 848
BtsCI GGATG 5 cut(s) 181, 203, 460, 553, 1072
BtsI GCAGTG 1 cut(s) 912
BtsIMutI CAGTG 3 cut(s) 280, 912, 1058
Cac8I GCNNGC 1 cut(s) 1291
CfoI GCGC 1 cut(s) 628
Cfr13I GGNCC 3 cut(s) 589, 976, 1171
CseI GACGC 2 cut(s) 524, 692
Csp6I GTAC 4 cut(s) 389, 631, 1003, 1051
CviAII CATG 8 cut(s) 31, 224, 271, 313, 445, 817, 841, 849
CviQI GTAC 4 cut(s) 389, 631, 1003, 1051
DdeI CTNAG 6 cut(s) 198, 339, 621, 729, 983, 1216
DpnI GATC 4 cut(s) 102, 599, 672, 810
DpnII GATC 4 cut(s) 100, 597, 670, 808
Eam1104I CTCTTC 1 cut(s) 437
EarI CTCTTC 1 cut(s) 437
Ecl136II GAGCTC 1 cut(s) 661
Eco130I CCWWGG 2 cut(s) 848, 971
Eco24I GRGCYC 2 cut(s) 416, 663
Eco47I GGWCC 3 cut(s) 589, 976, 1171
Eco53kI GAGCTC 1 cut(s) 661
Eco57I CTGAAG 1 cut(s) 569
Eco81I CCTNAGG 1 cut(s) 621
EcoICRI GAGCTC 1 cut(s) 661
EcoO109I RGGNCCY 1 cut(s) 589
EcoT14I CCWWGG 2 cut(s) 848, 971
EcoT38I GRGCYC 2 cut(s) 416, 663
ErhI CCWWGG 2 cut(s) 848, 971
FaeI CATG 8 cut(s) 34, 227, 274, 316, 448, 820, 844, 852
FaqI GGGAC 3 cut(s) 575, 989, 1184
FatI CATG 8 cut(s) 30, 223, 270, 312, 444, 816, 840, 848
FblI GTMKAC 1 cut(s) 957
Fnu4HI GCNGC 2 cut(s) 937, 1265
FokI GGATG 5 cut(s) 188, 190, 467, 540, 1059
FriOI GRGCYC 2 cut(s) 416, 663
Fsp4HI GCNGC 2 cut(s) 937, 1265
FspBI CTAG 1 cut(s) 1116
GlaI GCGC 1 cut(s) 627
GluI GCNGC 2 cut(s) 937, 1265
HgaI GACGC 2 cut(s) 524, 692
HhaI GCGC 1 cut(s) 628
Hin1I GRCGYC 2 cut(s) 535, 703
Hin1II CATG 8 cut(s) 34, 227, 274, 316, 448, 820, 844, 852
Hin6I GCGC 1 cut(s) 626
HinP1I GCGC 1 cut(s) 626
HincII GTYRAC 1 cut(s) 958
HindII GTYRAC 1 cut(s) 958
HinfI GANTC 6 cut(s) 316, 853, 1016, 1123, 1228, 1250
HphI GGTGA 1 cut(s) 320
Hpy166II GTNNAC 3 cut(s) 280, 857, 958
Hpy188I TCNGA 3 cut(s) 190, 497, 708
Hpy188III TCNNGA 6 cut(s) 104, 137, 467, 601, 686, 1247
Hpy8I GTNNAC 3 cut(s) 280, 857, 958
HpyAV CCTTC 4 cut(s) 268, 411, 570, 580
HpyCH4III ACNGT 7 cut(s) 22, 284, 493, 611, 749, 1046, 1062
HpyF3I CTNAG 6 cut(s) 198, 339, 621, 729, 983, 1216
Hsp92I GRCGYC 2 cut(s) 535, 703
Hsp92II CATG 8 cut(s) 34, 227, 274, 316, 448, 820, 844, 852
HspAI GCGC 1 cut(s) 626
Kzo9I GATC 4 cut(s) 100, 597, 670, 808
LguI GCTCTTC 1 cut(s) 437
LmnI GCTCC 5 cut(s) 658, 844, 890, 907, 1087
Lsp1109I GCAGC 1 cut(s) 923
LweI GCATC 4 cut(s) 371, 562, 757, 1081
MaeI CTAG 1 cut(s) 1116
MaeIII GTNAC 7 cut(s) 33, 234, 308, 487, 923, 1111, 1136
MalI GATC 4 cut(s) 102, 599, 672, 810
MboI GATC 4 cut(s) 100, 597, 670, 808
MboII GAAGA 5 cut(s) 424, 721, 803, 832, 874
MflI RGATCY 1 cut(s) 597
MhlI GDGCHC 4 cut(s) 282, 416, 663, 912
MluCI AATT 3 cut(s) 84, 406, 470
MlyI GAGTC 4 cut(s) 325, 862, 1132, 1222
MmeI TCCRAC 1 cut(s) 1100
MseI TTAA 2 cut(s) 1131, 1149
MslI CAYNNNNRTG 3 cut(s) 275, 449, 917
MspCI CTTAAG 1 cut(s) 1130
Mva1269I GAATGC 1 cut(s) 1090
NcoI CCATGG 1 cut(s) 848
NdeII GATC 4 cut(s) 100, 597, 670, 808
NlaIII CATG 8 cut(s) 34, 227, 274, 316, 448, 820, 844, 852
NlaIV GGNNCC 5 cut(s) 591, 846, 977, 1144, 1172
NmuCI GTSAC 2 cut(s) 234, 308
NspI RCATGY 1 cut(s) 34
NspV TTCGAA 1 cut(s) 295
PciI ACATGT 1 cut(s) 30
PciSI GCTCTTC 1 cut(s) 437
PcsI WCGNNNNNNNCGW 1 cut(s) 793
PctI GAATGC 1 cut(s) 1090
PfeI GAWTC 2 cut(s) 1016, 1250
PflMI CCANNNNNTGG 1 cut(s) 215
PkrI GCNGC 2 cut(s) 938, 1266
PleI GAGTC 4 cut(s) 324, 861, 1131, 1222
PpsI GAGTC 4 cut(s) 324, 861, 1131, 1222
PpuMI RGGWCCY 1 cut(s) 589
PscI ACATGT 1 cut(s) 30
PshAI GACNNNNGTC 1 cut(s) 315
PsiI TTATAA 1 cut(s) 476
Psp124BI GAGCTC 1 cut(s) 663
Psp5II RGGWCCY 1 cut(s) 589
PspN4I GGNNCC 5 cut(s) 591, 846, 977, 1144, 1172
PspPI GGNCC 3 cut(s) 589, 976, 1171
PspPPI RGGWCCY 1 cut(s) 589
PstI CTGCAG 1 cut(s) 325
PsuI RGATCY 1 cut(s) 597
RsaI GTAC 4 cut(s) 390, 632, 1004, 1052
RsaNI GTAC 4 cut(s) 389, 631, 1003, 1051
RseI CAYNNNNRTG 3 cut(s) 275, 449, 917
SacI GAGCTC 1 cut(s) 663
SalI GTCGAC 1 cut(s) 956
SapI GCTCTTC 1 cut(s) 437
SaqAI TTAA 2 cut(s) 1131, 1149
SatI GCNGC 2 cut(s) 937, 1265
Sau3AI GATC 4 cut(s) 100, 597, 670, 808
Sau96I GGNCC 3 cut(s) 589, 976, 1171
ScaI AGTACT 1 cut(s) 1052
SchI GAGTC 4 cut(s) 325, 862, 1132, 1222
SduI GDGCHC 4 cut(s) 282, 416, 663, 912
SfaNI GCATC 4 cut(s) 371, 562, 757, 1081
SfcI CTRYAG 2 cut(s) 321, 1269
SfuI TTCGAA 1 cut(s) 295
SinI GGWCC 3 cut(s) 589, 976, 1171
SmiMI CAYNNNNRTG 3 cut(s) 275, 449, 917
SmlI CTYRAG 1 cut(s) 1130
SmoI CTYRAG 1 cut(s) 1130
SpeI ACTAGT 1 cut(s) 1115
Sse9I AATT 3 cut(s) 84, 406, 470
SsiI CCGC 3 cut(s) 182, 778, 1265
SspMI CTAG 1 cut(s) 1116
SstI GAGCTC 1 cut(s) 663
StyI CCWWGG 2 cut(s) 848, 971
TaaI ACNGT 7 cut(s) 22, 284, 493, 611, 749, 1046, 1062
TaqI TCGA 3 cut(s) 295, 796, 957
TasI AATT 3 cut(s) 84, 406, 470
TatI WGTACW 3 cut(s) 630, 1002, 1050
TauI GCSGC 1 cut(s) 1267
TfiI GAWTC 2 cut(s) 1016, 1250
Tru1I TTAA 2 cut(s) 1131, 1149
Tru9I TTAA 2 cut(s) 1131, 1149
TscAI CASTG 3 cut(s) 287, 919, 1065
TseFI GTSAC 2 cut(s) 234, 308
TseI GCWGC 1 cut(s) 936
Tsp45I GTSAC 2 cut(s) 234, 308
TspDTI ATGAA 7 cut(s) 156, 240, 280, 287, 433, 582, 833
TspGWI ACGGA 1 cut(s) 1155
TspRI CASTG 3 cut(s) 287, 919, 1065
Van91I CCANNNNNTGG 1 cut(s) 215
Vha464I CTTAAG 1 cut(s) 1130
VneI GTGCAC 1 cut(s) 278
VpaK11BI GGWCC 3 cut(s) 589, 976, 1171
XapI RAATTY 2 cut(s) 406, 470
XceI RCATGY 1 cut(s) 34
XcmI CCANNNNNNNNNTGG 1 cut(s) 986
XmiI GTMKAC 1 cut(s) 957
XspI CTAG 1 cut(s) 1116
ZrmI AGTACT 1 cut(s) 1052
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.