FvH4_2g15630

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0006752
Physical Location & Seq
Reverse (-)
13717041 .. 13721100
4060 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g15630.t1

Sequence Viewer

Length: 1554 bp
ATGGAGAAAGATAGTCCACGGTCTGTATCGTTTCGCACTCACCGCAGAGGCGAAAGGAGTGGTTTAAGTGTGCTTGGTAGAGATGTTGAAGGTGGGATTTTTCCGGGAAAGGTTGCCCCGGATTCTGCCATCAAGATTGTTTGCAAGAAGGGCGTTATTCGATTGCTTCTTGTTGGTGTCATCTTGTGGGTGGTACTCATTCTCCTCGCAACTTTGTTCCATGTGTGGTCATGCCACTCTTCTATCTCAATCTTATCAGCTATGTGTAACAAAAATAGCACGGTGTTGGACATGTTACGCAATATGGGATTTGTAGCAAAACCACAACTCCAACACCGTTGTCCAATTCCCGTTGCCAATGATCCAGATAAGATAGTTATTCCAGAAGGAAGAACTCCTGATAAAATCGTTAAAAAGCTAATCTATGTGGAGGATCAGGCGGTAAATGACAAAACACAGTCATCCCCACTATTTGGAGGACATGAAAGCTGGTCGCAAAGAGAAGAGAGTTTTAAGCTAAAACCAACAATGAAGGTGCACTGTGGTTTCATTCGAAATGGTGGCGGTGACATGATTCCTGAAGACATTGAATATGCTGAGAAATGTAGGTTTGTGGTTGCATCGGGCATTTTTGATGGTTATGATATACCCCATCAACCTTCAAATTTGAGCCCCCGTTCTAAGAAGCTCTTCTGTTTTCTCATGGTGGTGGATGAGACATCTCTTGAATTTATAAAGGGTAATGTTACGATCAGAGAGGATAATAATGGAGGGAAATGGGTTGGCGTCTGGCGTCTAATTTTACTGAAGCATCCGCCTTATGATGAGCCAAGAAGGAATGGGAAGGTCCCCAAGATCATAACCCACCGTTTATTCCCTAAGGCGCAGTACAGCATTTGGATTGATGGTAAAATGGAGCTCATGGTTGATCCATTGCTTATACTGGAAAGATATTTATGGCGGGAGAAGAATACATTTGCCATTTCTCAGCACAAACATTCTCACAGTATATATGAGGAGGCAGATGCAAACAAGCGGAGGAAACGATATGCTCGACCTCTTATTGATCTTCACATGAAGATTTACCGATATGAGGGCATGGAGCCATGGAGTCCGCAGAAGAAAACTGTTAGTGATGTGCCAGAGGGAGCAATTATCATACGGGAGCATACTGCAATGAGTAACTTGTTTAGCTGCTTGTGGTTCAATGAGGTCGACCTCTTCACACCAAGGGACCAACTGAGTTTTGGTTATGTTCTGTACAGGTTAGGGGATGCGTTTAAGTTCTTTATGTTTCCAAACTGCGAGTACAATTCACTATTTATTTTGCATCCACACATAAGGGAGCATTCTTCTCGCATAGAGTGGGTAACTAGTTGGAGTCAGCTTAAGGGTAACGGAACCATTAAGGATAGGGGTAAAGATTGGGACCAAGCTAATGCGAAAAATGGTTTGGTAGAGAGTAGGGGAGGCTTAGGGTTGTGGACTCCTTATCCTGCAAATCTTGATTCAGTTGTCCTGCCGAAGGTAGAAAGAACTTCAAAAGCTGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616

Protein Analysis

518

Amino Acids

59.14

Weight (kDa)

9.26

Isoelectric Point (pI)

37.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOD1_MUCI70 PF04765 142 - 446 7.2e-130 TOD1/MUCI70, glycosyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 734
AccB7I CCANNNNNTGG 1 cut(s) 473
AccI GTMKAC 1 cut(s) 1215
AciI CCGC 7 cut(s) 43, 440, 564, 815, 961, 1036, 1115
AclWI GGATC 3 cut(s) 356, 441, 923
AcsI RAATTY 2 cut(s) 664, 728
AcuI CTGAAG 2 cut(s) 600, 827
AcyI GRCGYC 2 cut(s) 786, 793
AfaI GTAC 4 cut(s) 195, 890, 1262, 1310
AfiI CCNNNNNNNGG 3 cut(s) 473, 1093, 1525
AflII CTTAAG 1 cut(s) 1388
AflIII ACRYGT 1 cut(s) 291
AgsI TTSAA 6 cut(s) 89, 590, 663, 728, 1207, 1542
AhlI ACTAGT 1 cut(s) 1373
AjuI GAANNNNNNNTTGG 2 cut(s) 1436, 1468
Alw21I GWGCWC 2 cut(s) 540, 921
Alw26I GTCTC 1 cut(s) 710
Alw44I GTGCAC 1 cut(s) 536
AlwI GGATC 3 cut(s) 356, 441, 923
ApaLI GTGCAC 1 cut(s) 536
ApeKI GCWGC 1 cut(s) 1194
ApoI RAATTY 2 cut(s) 664, 728
Asp700I GAANNNNTTC 1 cut(s) 689
AspLEI GCGC 1 cut(s) 886
AspS9I GGNCC 3 cut(s) 847, 1234, 1429
AsuC2I CCSGG 2 cut(s) 105, 119
AsuHPI GGTGA 2 cut(s) 32, 578
AsuII TTCGAA 1 cut(s) 553
AvaII GGWCC 3 cut(s) 847, 1234, 1429
AxyI CCTNAGG 1 cut(s) 879
BaeGI GKGCMC 1 cut(s) 540
BanII GRGCYC 2 cut(s) 674, 921
BbsI GAAGAC 1 cut(s) 588
Bbv12I GWGCWC 2 cut(s) 540, 921
BbvI GCAGC 1 cut(s) 1181
BccI CCATC 4 cut(s) 137, 629, 660, 899
BcgI CGANNNNNNTGC 2 cut(s) 1337, 1371
BcnI CCSGG 2 cut(s) 105, 119
BcoDI GTCTC 1 cut(s) 710
BcuI ACTAGT 1 cut(s) 1373
BfaI CTAG 1 cut(s) 1374
BfrI CTTAAG 1 cut(s) 1388
BisI GCNGC 1 cut(s) 1195
BlsI GCNGC 1 cut(s) 1196
Bme1390I CCNGG 2 cut(s) 105, 119
Bme18I GGWCC 3 cut(s) 847, 1234, 1429
BmgT120I GGNCC 3 cut(s) 847, 1234, 1429
BmiI GGNNCC 5 cut(s) 849, 1104, 1235, 1402, 1430
BmrFI CCNGG 2 cut(s) 105, 119
BmsI GCATC 5 cut(s) 629, 820, 1015, 1264, 1339
BpiI GAAGAC 1 cut(s) 588
Bpu10I CCTNAGC 1 cut(s) 1474
Bpu14I TTCGAA 1 cut(s) 553
BpuMI CCSGG 2 cut(s) 105, 119
BsaHI GRCGYC 2 cut(s) 786, 793
BsaJI CCNNGG 4 cut(s) 17, 117, 1106, 1229
BsaXI ACNNNNNCTCC 4 cut(s) 186, 216, 312, 342
Bsc4I CCNNNNNNNGG 3 cut(s) 473, 1093, 1525
Bse1I ACTGG 1 cut(s) 948
Bse21I CCTNAGG 1 cut(s) 879
Bse3DI GCAATG 2 cut(s) 932, 1182
BseDI CCNNGG 4 cut(s) 17, 117, 1106, 1229
BseGI GGATG 5 cut(s) 461, 718, 811, 1279, 1330
BseLI CCNNNNNNNGG 3 cut(s) 473, 1093, 1525
BseMI GCAATG 2 cut(s) 932, 1182
BseMII CTCAG 3 cut(s) 588, 1001, 1232
BseNI ACTGG 1 cut(s) 948
BseRI GAGGAG 2 cut(s) 194, 1031
BseSI GKGCMC 1 cut(s) 540
BseXI GCAGC 1 cut(s) 1181
BsiHKAI GWGCWC 2 cut(s) 540, 921
BsiSI CCGG 2 cut(s) 104, 119
BslFI GGGAC 3 cut(s) 833, 1247, 1442
BslI CCNNNNNNNGG 3 cut(s) 473, 1093, 1525
BsmAI GTCTC 1 cut(s) 710
BsmFI GGGAC 3 cut(s) 833, 1247, 1442
BsmI GAATGC 1 cut(s) 1348
Bsp119I TTCGAA 1 cut(s) 553
Bsp1286I GDGCHC 3 cut(s) 540, 674, 921
Bsp1407I TGTACA 1 cut(s) 1260
Bsp143I GATC 6 cut(s) 361, 433, 750, 855, 928, 1066
Bsp19I CCATGG 1 cut(s) 1106
BspACI CCGC 7 cut(s) 43, 440, 564, 815, 961, 1036, 1115
BspCNI CTCAG 3 cut(s) 589, 1000, 1233
BspLI GGNNCC 5 cut(s) 849, 1104, 1235, 1402, 1430
BspPI GGATC 3 cut(s) 356, 441, 923
BspQI GCTCTTC 1 cut(s) 695
BspT104I TTCGAA 1 cut(s) 553
BspTI CTTAAG 1 cut(s) 1388
BsrDI GCAATG 2 cut(s) 932, 1182
BsrGI TGTACA 1 cut(s) 1260
BsrI ACTGG 1 cut(s) 948
BssECI CCNNGG 4 cut(s) 17, 117, 1106, 1229
BssMI GATC 6 cut(s) 361, 433, 750, 855, 928, 1066
BssNI GRCGYC 2 cut(s) 786, 793
BssT1I CCWWGG 2 cut(s) 1106, 1229
Bst4CI ACNGT 8 cut(s) 21, 283, 338, 459, 542, 869, 1007, 1129
Bst6I CTCTTC 4 cut(s) 244, 498, 695, 1226
BstACI GRCGYC 2 cut(s) 786, 793
BstAFI CTTAAG 1 cut(s) 1388
BstAUI TGTACA 1 cut(s) 1260
BstBI TTCGAA 1 cut(s) 553
BstC8I GCNNGC 1 cut(s) 1549
BstDEI CTNAG 6 cut(s) 597, 681, 879, 987, 1241, 1474
BstDSI CCRYGG 2 cut(s) 17, 1106
BstENI CCTNNNNNAGG 1 cut(s) 1523
BstF5I GGATG 5 cut(s) 461, 718, 811, 1279, 1330
BstHHI GCGC 1 cut(s) 886
BstKTI GATC 6 cut(s) 364, 436, 753, 858, 931, 1069
BstMAI GTCTC 1 cut(s) 710
BstMBI GATC 6 cut(s) 361, 433, 750, 855, 928, 1066
BstMWI GCNNNNNNNGC 2 cut(s) 42, 150
BstNSI RCATGY 1 cut(s) 295
BstSCI CCNGG 2 cut(s) 103, 117
BstSLI GKGCMC 1 cut(s) 540
BstV1I GCAGC 1 cut(s) 1181
BstV2I GAAGAC 1 cut(s) 588
Bsu36I CCTNAGG 1 cut(s) 879
BtgI CCRYGG 2 cut(s) 17, 1106
BtsCI GGATG 5 cut(s) 461, 718, 811, 1279, 1330
BtsIMutI CAGTG 1 cut(s) 538
Cac8I GCNNGC 1 cut(s) 1549
CfoI GCGC 1 cut(s) 886
Cfr13I GGNCC 3 cut(s) 847, 1234, 1429
CseI GACGC 2 cut(s) 775, 782
Csp6I GTAC 4 cut(s) 194, 889, 1261, 1309
CviQI GTAC 4 cut(s) 194, 889, 1261, 1309
DdeI CTNAG 6 cut(s) 597, 681, 879, 987, 1241, 1474
DpnI GATC 6 cut(s) 363, 435, 752, 857, 930, 1068
DpnII GATC 6 cut(s) 361, 433, 750, 855, 928, 1066
Eam1104I CTCTTC 4 cut(s) 244, 498, 695, 1226
EarI CTCTTC 4 cut(s) 244, 498, 695, 1226
EciI GGCGGA 1 cut(s) 804
Ecl136II GAGCTC 1 cut(s) 919
Eco130I CCWWGG 2 cut(s) 1106, 1229
Eco24I GRGCYC 2 cut(s) 674, 921
Eco47I GGWCC 3 cut(s) 847, 1234, 1429
Eco53kI GAGCTC 1 cut(s) 919
Eco57I CTGAAG 2 cut(s) 600, 827
Eco81I CCTNAGG 1 cut(s) 879
EcoICRI GAGCTC 1 cut(s) 919
EcoNI CCTNNNNNAGG 1 cut(s) 1523
EcoO109I RGGNCCY 1 cut(s) 847
EcoT14I CCWWGG 2 cut(s) 1106, 1229
EcoT38I GRGCYC 2 cut(s) 674, 921
ErhI CCWWGG 2 cut(s) 1106, 1229
FalI AAGNNNNNCTT 2 cut(s) 674, 706
FaqI GGGAC 3 cut(s) 833, 1247, 1442
FauI CCCGC 1 cut(s) 954
FblI GTMKAC 1 cut(s) 1215
Fnu4HI GCNGC 1 cut(s) 1195
FokI GGATG 5 cut(s) 448, 725, 798, 1286, 1317
FriOI GRGCYC 2 cut(s) 674, 921
Fsp4HI GCNGC 1 cut(s) 1195
FspBI CTAG 1 cut(s) 1374
GlaI GCGC 1 cut(s) 885
GluI GCNGC 1 cut(s) 1195
HapII CCGG 2 cut(s) 104, 119
HgaI GACGC 2 cut(s) 775, 782
HhaI GCGC 1 cut(s) 886
Hin1I GRCGYC 2 cut(s) 786, 793
Hin6I GCGC 1 cut(s) 884
HinP1I GCGC 1 cut(s) 884
HincII GTYRAC 1 cut(s) 1216
HindII GTYRAC 1 cut(s) 1216
HinfI GANTC 6 cut(s) 122, 574, 1111, 1381, 1486, 1508
HpaII CCGG 2 cut(s) 104, 119
HphI GGTGA 2 cut(s) 32, 578
Hpy166II GTNNAC 4 cut(s) 17, 538, 1216, 1485
Hpy188I TCNGA 1 cut(s) 755
Hpy188III TCNNGA 7 cut(s) 133, 365, 383, 398, 578, 725, 1505
Hpy8I GTNNAC 4 cut(s) 17, 538, 1216, 1485
HpyAV CCTTC 8 cut(s) 83, 142, 380, 526, 669, 828, 838, 1519
HpyCH4III ACNGT 8 cut(s) 21, 283, 338, 459, 542, 869, 1007, 1129
HpyCH4V TGCA 7 cut(s) 144, 538, 620, 1028, 1175, 1330, 1499
HpyF10VI GCNNNNNNNGC 2 cut(s) 42, 150
HpyF3I CTNAG 6 cut(s) 597, 681, 879, 987, 1241, 1474
Hsp92I GRCGYC 2 cut(s) 786, 793
HspAI GCGC 1 cut(s) 884
Kzo9I GATC 6 cut(s) 361, 433, 750, 855, 928, 1066
LguI GCTCTTC 1 cut(s) 695
LmnI GCTCC 5 cut(s) 916, 1102, 1148, 1165, 1345
Lsp1109I GCAGC 1 cut(s) 1181
LweI GCATC 5 cut(s) 629, 820, 1015, 1264, 1339
MaeI CTAG 1 cut(s) 1374
MaeIII GTNAC 7 cut(s) 266, 294, 566, 745, 1181, 1369, 1394
MalI GATC 6 cut(s) 363, 435, 752, 857, 930, 1068
MboI GATC 6 cut(s) 361, 433, 750, 855, 928, 1066
MhlI GDGCHC 3 cut(s) 540, 674, 921
MluCI AATT 6 cut(s) 345, 664, 728, 798, 1152, 1312
MlyI GAGTC 3 cut(s) 1120, 1390, 1480
MmeI TCCRAC 3 cut(s) 267, 355, 1358
MroXI GAANNNNTTC 1 cut(s) 689
MseI TTAA 6 cut(s) 65, 411, 513, 1281, 1389, 1407
MslI CAYNNNNRTG 1 cut(s) 707
MspCI CTTAAG 1 cut(s) 1388
MspI CCGG 2 cut(s) 104, 119
MspR9I CCNGG 2 cut(s) 105, 119
Mva1269I GAATGC 1 cut(s) 1348
MwoI GCNNNNNNNGC 2 cut(s) 42, 150
NciI CCSGG 2 cut(s) 105, 119
NcoI CCATGG 1 cut(s) 1106
NdeII GATC 6 cut(s) 361, 433, 750, 855, 928, 1066
NlaIV GGNNCC 5 cut(s) 849, 1104, 1235, 1402, 1430
NmuCI GTSAC 1 cut(s) 566
NspI RCATGY 1 cut(s) 295
NspV TTCGAA 1 cut(s) 553
PciI ACATGT 1 cut(s) 291
PciSI GCTCTTC 1 cut(s) 695
PcsI WCGNNNNNNNCGW 1 cut(s) 1051
PctI GAATGC 1 cut(s) 1348
PdmI GAANNNNTTC 1 cut(s) 689
PfeI GAWTC 3 cut(s) 122, 574, 1508
PflMI CCANNNNNTGG 1 cut(s) 473
PfoI TCCNGGA 1 cut(s) 103
PkrI GCNGC 1 cut(s) 1196
PleI GAGTC 3 cut(s) 1119, 1389, 1480
PpsI GAGTC 3 cut(s) 1119, 1389, 1480
PpuMI RGGWCCY 1 cut(s) 847
PscI ACATGT 1 cut(s) 291
PsiI TTATAA 1 cut(s) 734
Psp124BI GAGCTC 1 cut(s) 921
Psp5II RGGWCCY 1 cut(s) 847
PspN4I GGNNCC 5 cut(s) 849, 1104, 1235, 1402, 1430
PspPI GGNCC 3 cut(s) 847, 1234, 1429
PspPPI RGGWCCY 1 cut(s) 847
RsaI GTAC 4 cut(s) 195, 890, 1262, 1310
RsaNI GTAC 4 cut(s) 194, 889, 1261, 1309
RseI CAYNNNNRTG 1 cut(s) 707
SacI GAGCTC 1 cut(s) 921
SalI GTCGAC 1 cut(s) 1214
SapI GCTCTTC 1 cut(s) 695
SaqAI TTAA 6 cut(s) 65, 411, 513, 1281, 1389, 1407
SatI GCNGC 1 cut(s) 1195
Sau3AI GATC 6 cut(s) 361, 433, 750, 855, 928, 1066
Sau96I GGNCC 3 cut(s) 847, 1234, 1429
SchI GAGTC 3 cut(s) 1120, 1390, 1480
ScrFI CCNGG 2 cut(s) 105, 119
SduI GDGCHC 3 cut(s) 540, 674, 921
SfaNI GCATC 5 cut(s) 629, 820, 1015, 1264, 1339
SfuI TTCGAA 1 cut(s) 553
SinI GGWCC 3 cut(s) 847, 1234, 1429
SmiMI CAYNNNNRTG 1 cut(s) 707
SmlI CTYRAG 1 cut(s) 1388
SmoI CTYRAG 1 cut(s) 1388
SpeI ACTAGT 1 cut(s) 1373
Sse9I AATT 6 cut(s) 345, 664, 728, 798, 1152, 1312
SsiI CCGC 7 cut(s) 43, 440, 564, 815, 961, 1036, 1115
SspMI CTAG 1 cut(s) 1374
SstI GAGCTC 1 cut(s) 921
StyD4I CCNGG 2 cut(s) 103, 117
StyI CCWWGG 2 cut(s) 1106, 1229
TaaI ACNGT 8 cut(s) 21, 283, 338, 459, 542, 869, 1007, 1129
TaqI TCGA 4 cut(s) 160, 553, 1054, 1215
TasI AATT 6 cut(s) 345, 664, 728, 798, 1152, 1312
TatI WGTACW 3 cut(s) 888, 1260, 1308
TfiI GAWTC 3 cut(s) 122, 574, 1508
Tru1I TTAA 6 cut(s) 65, 411, 513, 1281, 1389, 1407
Tru9I TTAA 6 cut(s) 65, 411, 513, 1281, 1389, 1407
TscAI CASTG 1 cut(s) 545
TseFI GTSAC 1 cut(s) 566
TseI GCWGC 1 cut(s) 1194
Tsp45I GTSAC 1 cut(s) 566
TspDTI ATGAA 4 cut(s) 498, 538, 545, 1091
TspGWI ACGGA 1 cut(s) 1413
TspRI CASTG 1 cut(s) 545
Van91I CCANNNNNTGG 1 cut(s) 473
Vha464I CTTAAG 1 cut(s) 1388
VneI GTGCAC 1 cut(s) 536
VpaK11BI GGWCC 3 cut(s) 847, 1234, 1429
XagI CCTNNNNNAGG 1 cut(s) 1523
XapI RAATTY 2 cut(s) 664, 728
XceI RCATGY 1 cut(s) 295
XcmI CCANNNNNNNNNTGG 1 cut(s) 1244
XmiI GTMKAC 1 cut(s) 1215
XmnI GAANNNNTTC 1 cut(s) 689
XspI CTAG 1 cut(s) 1374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.