MD00G1148400.v1.1

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0006752
Physical Location & Seq
Forward (+)
32574940 .. 32577432
2493 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1148400.v1.1.491

Sequence Viewer

Length: 1095 bp
ATGGATAAAGAGAGTCCGCGGTCTATTTCTTTTCGCACACATCGTAGAGGCGAAAGGAGTAATTTAAACCAACATAGTAAAGATGTTGAAGAGGGATTCCCTCTGGGAAGGGTGACACAGGATTATCCCATGAAGATTGTTTGGAAGAAGGGCTTTATTCGATTGGTTCTTGTAGGTGGCATTTTGTGGATGCTACTAATTCTCCTCGCGTCATTATTTCATGTTTGGTCCTGCCAGTCTTCTATCTCTTTCTTCTCAGGCTGTCCGATTCCTGTTGTCAGTGATCCTGATAAGATAAAAATTCCAAAAGGAAGAACTCCTGATGAAATTGTCAAAAACTTAACATACGTTTTAGATGATGAGATTTTGCCTGACGGAACTCTGTCATCCCCACTATTCGGAGGACATCAAAATTGGGCTCAGAGAGAGGAGAGTTTTAGACTAAAATCTGACATGAAGTTGCACTGTGGTTTTATTCGAAACGGTGGTGCTGAAATGGCTCCTGCAGACATCAACTATGCCAAAAAATGTAGGTTTGTGGTTGCATCAGGCATTTTTGATGGTTATGATGTACCCCATCAACCTTCAAATTTAAGTCCTCGTTCTAAGAAGCTCTTCTGCTTTCTCATGGCGGTCGATGAAACATCTCTTGAATTTATCAAGGGAAATGTGACAGTCAGAGAGGATAATGATGGGGGAAAATGGGTTGGTATCTGGCGTCTTGTTTTGTTGAAGCATCCGCCTTACGATGAGCCAAGAAGGAACGGGAAGGTCCCCAAGATATTAACCCACCGCATGTTCCCTAACACCCAGTACAGCATTTGGATCGATGGTAAAATGGAGCTCATGGTTGATCCATTGCAAATTTTAGAAAGATATTTATGGCGTGGGAAGCATACATTTGCCATTTCTCAGCACAAGCACCACCGCAGTATATACGAGGAGGCTGATGCAAACAAGCGGAGGAAGCGATATGCTCGACCTCTTATCGATCTACACATGAAAATTTATCGTTATGAGGGTTTGGAGGCATGGGATCCAACCAAGAAAACCTTCTGTTTATTTGTTTCTCCTCCGCTCATTTTTATAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616

Protein Analysis

365

Amino Acids

42.16

Weight (kDa)

9.39

Isoelectric Point (pI)

42.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOD1_MUCI70 PF04765 114 - 349 2.9e-91 TOD1/MUCI70, glycosyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1088
AccBSI CCGCTC 1 cut(s) 1078
AccII CGCG 2 cut(s) 19, 209
AciI CCGC 8 cut(s) 17, 19, 632, 740, 793, 928, 961, 1076
AclWI GGATC 5 cut(s) 278, 833, 848, 1031, 1044
AcsI RAATTY 5 cut(s) 300, 589, 653, 864, 1005
AcyI GRCGYC 1 cut(s) 718
AfaI GTAC 2 cut(s) 573, 815
AfiI CCNNNNNNNGG 1 cut(s) 398
AgsI TTSAA 4 cut(s) 89, 588, 653, 733
AjuI GAANNNNNNNTTGG 2 cut(s) 1037, 1069
AluBI AGCT 2 cut(s) 613, 844
AluI AGCT 2 cut(s) 613, 844
Alw21I GWGCWC 1 cut(s) 846
AlwI GGATC 5 cut(s) 278, 833, 848, 1031, 1044
ApoI RAATTY 5 cut(s) 300, 589, 653, 864, 1005
Asp700I GAANNNNTTC 2 cut(s) 614, 1052
AspS9I GGNCC 2 cut(s) 228, 772
AsuHPI GGTGA 1 cut(s) 124
AsuII TTCGAA 1 cut(s) 478
AvaII GGWCC 2 cut(s) 228, 772
BamHI GGATCC 1 cut(s) 1036
BanII GRGCYC 2 cut(s) 421, 846
BbsI GAAGAC 1 cut(s) 231
Bbv12I GWGCWC 1 cut(s) 846
BccI CCATC 4 cut(s) 554, 585, 686, 824
BcgI CGANNNNNNTGC 2 cut(s) 808, 842
BfmI CTRYAG 1 cut(s) 504
Bme18I GGWCC 2 cut(s) 228, 772
BmgT120I GGNCC 2 cut(s) 228, 772
BmiI GGNNCC 3 cut(s) 501, 774, 1038
BmrI ACTGGG 1 cut(s) 805
BmsI GCATC 4 cut(s) 180, 554, 745, 940
BmuI ACTGGG 1 cut(s) 805
BpiI GAAGAC 1 cut(s) 231
Bpu14I TTCGAA 1 cut(s) 478
Bsa29I ATCGAT 2 cut(s) 828, 990
BsaHI GRCGYC 1 cut(s) 718
BsaJI CCNNGG 1 cut(s) 17
BsaXI ACNNNNNCTCC 2 cut(s) 186, 216
Bsc4I CCNNNNNNNGG 1 cut(s) 398
Bse1I ACTGG 2 cut(s) 235, 811
Bse3DI GCAATG 1 cut(s) 857
BseCI ATCGAT 2 cut(s) 828, 990
BseDI CCNNGG 1 cut(s) 17
BseGI GGATG 3 cut(s) 195, 386, 736
BseLI CCNNNNNNNGG 1 cut(s) 398
BseMI GCAATG 1 cut(s) 857
BseMII CTCAG 3 cut(s) 270, 434, 926
BseNI ACTGG 2 cut(s) 235, 811
BseRI GAGGAG 4 cut(s) 194, 443, 956, 1062
Bsh1236I CGCG 2 cut(s) 19, 209
Bsh1285I CGRYCG 1 cut(s) 636
BshVI ATCGAT 2 cut(s) 828, 990
BsiEI CGRYCG 1 cut(s) 636
BsiHKAI GWGCWC 1 cut(s) 846
BslFI GGGAC 1 cut(s) 758
BslI CCNNNNNNNGG 1 cut(s) 398
BsmFI GGGAC 1 cut(s) 758
Bsp119I TTCGAA 1 cut(s) 478
Bsp1286I GDGCHC 2 cut(s) 421, 846
Bsp143I GATC 5 cut(s) 283, 825, 853, 991, 1036
BspACI CCGC 8 cut(s) 17, 19, 632, 740, 793, 928, 961, 1076
BspCNI CTCAG 3 cut(s) 269, 433, 925
BspDI ATCGAT 2 cut(s) 828, 990
BspFNI CGCG 2 cut(s) 19, 209
BspLI GGNNCC 3 cut(s) 501, 774, 1038
BspMAI CTGCAG 1 cut(s) 508
BspPI GGATC 5 cut(s) 278, 833, 848, 1031, 1044
BspQI GCTCTTC 1 cut(s) 620
BspT104I TTCGAA 1 cut(s) 478
BsrBI CCGCTC 1 cut(s) 1078
BsrDI GCAATG 1 cut(s) 857
BsrI ACTGG 2 cut(s) 235, 811
BssECI CCNNGG 1 cut(s) 17
BssMI GATC 5 cut(s) 283, 825, 853, 991, 1036
BssNI GRCGYC 1 cut(s) 718
Bst4CI ACNGT 3 cut(s) 467, 485, 676
Bst6I CTCTTC 2 cut(s) 84, 620
BstACI GRCGYC 1 cut(s) 718
BstBI TTCGAA 1 cut(s) 478
BstDEI CTNAG 4 cut(s) 256, 420, 606, 912
BstDSI CCRYGG 1 cut(s) 17
BstF5I GGATG 3 cut(s) 195, 386, 736
BstFNI CGCG 2 cut(s) 19, 209
BstKTI GATC 5 cut(s) 286, 828, 856, 994, 1039
BstMBI GATC 5 cut(s) 283, 825, 853, 991, 1036
BstMCI CGRYCG 1 cut(s) 636
BstMWI GCNNNNNNNGC 3 cut(s) 497, 892, 967
BstNSI RCATGY 1 cut(s) 799
BstSFI CTRYAG 1 cut(s) 504
BstUI CGCG 2 cut(s) 19, 209
BstV2I GAAGAC 1 cut(s) 231
BstX2I RGATCY 1 cut(s) 1036
BstYI RGATCY 1 cut(s) 1036
Bsu15I ATCGAT 2 cut(s) 828, 990
BsuTUI ATCGAT 2 cut(s) 828, 990
BtgI CCRYGG 1 cut(s) 17
BtsCI GGATG 3 cut(s) 195, 386, 736
BtsIMutI CAGTG 2 cut(s) 286, 463
Cfr13I GGNCC 2 cut(s) 228, 772
Cfr42I CCGCGG 1 cut(s) 20
ClaI ATCGAT 2 cut(s) 828, 990
CseI GACGC 2 cut(s) 198, 707
Csp6I GTAC 2 cut(s) 572, 814
CviAII CATG 8 cut(s) 130, 221, 454, 628, 796, 847, 1000, 1032
CviJI RGCY 8 cut(s) 153, 261, 419, 500, 613, 754, 844, 947
CviKI_1 RGCY 8 cut(s) 153, 261, 419, 500, 613, 754, 844, 947
CviQI GTAC 2 cut(s) 572, 814
DdeI CTNAG 4 cut(s) 256, 420, 606, 912
DpnI GATC 5 cut(s) 285, 827, 855, 993, 1038
DpnII GATC 5 cut(s) 283, 825, 853, 991, 1036
DraI TTTAAA 1 cut(s) 66
Eam1104I CTCTTC 2 cut(s) 84, 620
EarI CTCTTC 2 cut(s) 84, 620
EciI GGCGGA 1 cut(s) 729
Ecl136II GAGCTC 1 cut(s) 844
Eco24I GRGCYC 2 cut(s) 421, 846
Eco47I GGWCC 2 cut(s) 228, 772
Eco53kI GAGCTC 1 cut(s) 844
EcoICRI GAGCTC 1 cut(s) 844
EcoO109I RGGNCCY 1 cut(s) 772
EcoT38I GRGCYC 2 cut(s) 421, 846
FaeI CATG 8 cut(s) 133, 224, 457, 631, 799, 850, 1003, 1035
FalI AAGNNNNNCTT 6 cut(s) 137, 169, 599, 631, 1037, 1069
FaqI GGGAC 1 cut(s) 758
FatI CATG 8 cut(s) 129, 220, 453, 627, 795, 846, 999, 1031
FokI GGATG 3 cut(s) 202, 373, 723
FriOI GRGCYC 2 cut(s) 421, 846
HgaI GACGC 2 cut(s) 198, 707
Hin1I GRCGYC 1 cut(s) 718
Hin1II CATG 8 cut(s) 133, 224, 457, 631, 799, 850, 1003, 1035
HinfI GANTC 3 cut(s) 13, 96, 268
HphI GGTGA 1 cut(s) 124
Hpy188I TCNGA 5 cut(s) 267, 401, 423, 451, 680
Hpy188III TCNNGA 3 cut(s) 287, 320, 650
HpyAV CCTTC 6 cut(s) 102, 142, 594, 753, 763, 1063
HpyCH4III ACNGT 3 cut(s) 467, 485, 676
HpyCH4IV ACGT 1 cut(s) 348
HpyCH4V TGCA 5 cut(s) 463, 506, 545, 862, 953
HpyF10VI GCNNNNNNNGC 3 cut(s) 497, 892, 967
HpyF3I CTNAG 4 cut(s) 256, 420, 606, 912
HpySE526I ACGT 1 cut(s) 348
Hsp92I GRCGYC 1 cut(s) 718
Hsp92II CATG 8 cut(s) 133, 224, 457, 631, 799, 850, 1003, 1035
KspI CCGCGG 1 cut(s) 20
Kzo9I GATC 5 cut(s) 283, 825, 853, 991, 1036
LguI GCTCTTC 1 cut(s) 620
LmnI GCTCC 2 cut(s) 505, 841
LweI GCATC 4 cut(s) 180, 554, 745, 940
MaeII ACGT 1 cut(s) 348
MaeIII GTNAC 2 cut(s) 112, 670
MalI GATC 5 cut(s) 285, 827, 855, 993, 1038
MbiI CCGCTC 1 cut(s) 1078
MboI GATC 5 cut(s) 283, 825, 853, 991, 1036
MboII GAAGA 7 cut(s) 101, 145, 157, 231, 244, 324, 607
MflI RGATCY 1 cut(s) 1036
MhlI GDGCHC 2 cut(s) 421, 846
MluCI AATT 9 cut(s) 61, 198, 300, 327, 412, 589, 653, 864, 1005
MlyI GAGTC 1 cut(s) 22
MmeI TCCRAC 1 cut(s) 1064
MroXI GAANNNNTTC 2 cut(s) 614, 1052
MseI TTAA 4 cut(s) 65, 341, 593, 785
MspA1I CMGCKG 1 cut(s) 19
MvnI CGCG 2 cut(s) 19, 209
MwoI GCNNNNNNNGC 3 cut(s) 497, 892, 967
NdeII GATC 5 cut(s) 283, 825, 853, 991, 1036
NlaIII CATG 8 cut(s) 133, 224, 457, 631, 799, 850, 1003, 1035
NlaIV GGNNCC 3 cut(s) 501, 774, 1038
NmuCI GTSAC 2 cut(s) 112, 670
NspI RCATGY 1 cut(s) 799
NspV TTCGAA 1 cut(s) 478
PciSI GCTCTTC 1 cut(s) 620
PcsI WCGNNNNNNNCGW 1 cut(s) 40
PdmI GAANNNNTTC 2 cut(s) 614, 1052
PfeI GAWTC 2 cut(s) 96, 268
PleI GAGTC 1 cut(s) 21
PpsI GAGTC 1 cut(s) 21
PpuMI RGGWCCY 1 cut(s) 772
PsiI TTATAA 1 cut(s) 1088
Psp124BI GAGCTC 1 cut(s) 846
Psp5II RGGWCCY 1 cut(s) 772
PspN4I GGNNCC 3 cut(s) 501, 774, 1038
PspPI GGNCC 2 cut(s) 228, 772
PspPPI RGGWCCY 1 cut(s) 772
PstI CTGCAG 1 cut(s) 508
PsuI RGATCY 1 cut(s) 1036
RsaI GTAC 2 cut(s) 573, 815
RsaNI GTAC 2 cut(s) 572, 814
SacI GAGCTC 1 cut(s) 846
SacII CCGCGG 1 cut(s) 20
SapI GCTCTTC 1 cut(s) 620
SaqAI TTAA 4 cut(s) 65, 341, 593, 785
Sau3AI GATC 5 cut(s) 283, 825, 853, 991, 1036
Sau96I GGNCC 2 cut(s) 228, 772
SchI GAGTC 1 cut(s) 22
SduI GDGCHC 2 cut(s) 421, 846
SetI ASST 9 cut(s) 178, 351, 536, 586, 615, 774, 846, 985, 1055
SfaNI GCATC 4 cut(s) 180, 554, 745, 940
SfcI CTRYAG 1 cut(s) 504
Sfr303I CCGCGG 1 cut(s) 20
SfuI TTCGAA 1 cut(s) 478
SgrBI CCGCGG 1 cut(s) 20
SinI GGWCC 2 cut(s) 228, 772
Sse9I AATT 9 cut(s) 61, 198, 300, 327, 412, 589, 653, 864, 1005
SsiI CCGC 8 cut(s) 17, 19, 632, 740, 793, 928, 961, 1076
SstI GAGCTC 1 cut(s) 846
TaaI ACNGT 3 cut(s) 467, 485, 676
TaiI ACGT 1 cut(s) 351
TaqI TCGA 6 cut(s) 160, 478, 636, 828, 979, 990
TasI AATT 9 cut(s) 61, 198, 300, 327, 412, 589, 653, 864, 1005
TatI WGTACW 1 cut(s) 813
TfiI GAWTC 2 cut(s) 96, 268
Tru1I TTAA 4 cut(s) 65, 341, 593, 785
Tru9I TTAA 4 cut(s) 65, 341, 593, 785
TscAI CASTG 2 cut(s) 286, 470
TseFI GTSAC 2 cut(s) 112, 670
Tsp45I GTSAC 2 cut(s) 112, 670
TspDTI ATGAA 6 cut(s) 146, 209, 339, 470, 654, 1016
TspGWI ACGGA 1 cut(s) 390
TspRI CASTG 2 cut(s) 286, 470
VpaK11BI GGWCC 2 cut(s) 228, 772
XapI RAATTY 5 cut(s) 300, 589, 653, 864, 1005
XceI RCATGY 1 cut(s) 799
XmnI GAANNNNTTC 2 cut(s) 614, 1052
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.