RLG00000013191

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0006752
Physical Location & Seq
Forward (+)
27433805 .. 27436736
2932 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013191

Sequence Viewer

Length: 1557 bp
ATGGAGAATAGTAGTCCACGGTCTGTGTCTTTTCGCACGCACCGAAGAGGCGAAAGGAGTGGTCTAAGCGTGCTTAGTAAAGATGTTGAAGGTGGAGTTTTTCCAGGGAAGGTTGCCCCAGATTCTGCCATGAAGATTGTTTGCAAGAAGGGCGTTATTCGATTGCTTCTTGTTGGTGTCATCTTGTGGGTGGTACTAATTCTCCTCGCAACTTTGTTCCACGTTTGGTCCTGCCACTCTTCTATCTCATTCTTATCAGCTATGTGCAACAAAAATAGCACGGTATTGGACATGTTACGCAATATGGGATTTGTAGCAAAACCACAACTGCAACACCGTTGTCGAATTCCCGTTGCCAATGATCCTGATAAGATAGTTATACCAGAGGGGAGAACTCCTGATGAAATCGTCAAAAAGCTAACCTATGTTATGGAGGATGAGGCGGCATCTGACAAAACTCAGTCATCCCCGCTATTTGGAGGACATGAAAGCTGGTCACAAAGAGAAAAGAGTTTTAGACTAAAACCAACCATGAAGGTGCACTGTGGTTTCATTCGAAATGGTGGTGGTGACATGATTCCTGCAGACATTGAATATGCTGAGAAATGTAGGTTTGTGGTTGCATCGGGCATTTTTGATGGTTATGATGTACCCCATCAACCTTCAAATTTGAGCCCCCGTTCTAAAAAGCTCTTCTGTTTTTTCATGGTGGTGGATGAGACATCTCTTGAATTTATAAGTAAAAATGTTACTGTCAGAGAGGATAATAATGGGGGGAAATGGGTTGGCATTTGGCGTCTGGTTTTACTGAAGCATCCACCTTATGATGAACCAAGAAGGAATGGGAAGGTCCCCAAGATCTTGACCCACCGTTTATTCCCTAAGGCACAGTACAGCATTTGGATTGATGGTAAAATGGAGCTCGTAGTTGATCCATTGCTTATTCTGGAAAGATATTTATGGCGTGAGAAGAATACATTTGCCATTTCTCAGCACAAACATCATCACAGTATATATGAGGAGGCAGATGCAAACAAGCGGAGGAAACGATATGCTCGACCTCTTATTGATCTTCACATGAAGATTTACCGATATGAGGGCATGGAGCCATGGAGTCCGCAGAAGAAAACTATTAGTGATGTGCCAGAGGGAGCTATTATCATACGGGAGCATACTGCAATGAGTAACTTGTTTAGCTGCTTGTGGTTCAATGAGGTCGACCTCTTTACACCAAGGGACCAACTGAGTTTTGGTTATGTTCTGTACAGGTTGGGGGATTCGTTTAGGTTCTTTATGTTTCCAAACTGTGAGTACTATTCACTGTTTATTTTGCATCCTCACATAAGGGAGCATTCCTCTCGCATCGAGTGGGTAACTAGTTGGAGTCAGCTTAAGGGTAACGGAACCATTAAGGATAAGGGTAAGGATTGGGATCAAGCTAATGCAAAAAATGGTTTGGTAGAGAGTAGGGGAGGCTTAGGGTTATGGACTCCTTATCCTGCAAATCTTGATTCAGTTATCCTGCCACCTGTAGCAAGAACATCAAAAGCTGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616

Protein Analysis

519

Amino Acids

59.29

Weight (kDa)

9.31

Isoelectric Point (pI)

39.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOD1_MUCI70 PF04765 140 - 447 8e-132 TOD1/MUCI70, glycosyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 737
AccI GTMKAC 1 cut(s) 1218
AciI CCGC 4 cut(s) 443, 470, 1039, 1118
AclWI GGATC 3 cut(s) 356, 926, 1440
AcsI RAATTY 3 cut(s) 345, 667, 731
AcuI CTGAAG 1 cut(s) 830
AcyI GRCGYC 1 cut(s) 796
AfaI GTAC 5 cut(s) 195, 651, 893, 1265, 1313
AfiI CCNNNNNNNGG 2 cut(s) 476, 1096
AflII CTTAAG 1 cut(s) 1391
AflIII ACRYGT 1 cut(s) 291
AgsI TTSAA 5 cut(s) 89, 593, 666, 731, 1210
AhlI ACTAGT 1 cut(s) 1376
AjnI CCWGG 1 cut(s) 103
Alw21I GWGCWC 2 cut(s) 543, 924
Alw26I GTCTC 1 cut(s) 713
Alw44I GTGCAC 1 cut(s) 539
AlwI GGATC 3 cut(s) 356, 926, 1440
AlwNI CAGNNNCTG 1 cut(s) 125
ApaLI GTGCAC 1 cut(s) 539
ApeKI GCWGC 1 cut(s) 1197
ApoI RAATTY 3 cut(s) 345, 667, 731
AspS9I GGNCC 3 cut(s) 228, 850, 1237
AsuHPI GGTGA 1 cut(s) 581
AsuII TTCGAA 1 cut(s) 556
AvaII GGWCC 3 cut(s) 228, 850, 1237
AxyI CCTNAGG 1 cut(s) 882
BaeGI GKGCMC 1 cut(s) 543
BanII GRGCYC 2 cut(s) 677, 924
Bbv12I GWGCWC 2 cut(s) 543, 924
BbvI GCAGC 1 cut(s) 1184
BccI CCATC 3 cut(s) 632, 663, 902
BcgI CGANNNNNNTGC 2 cut(s) 1340, 1374
BciT130I CCWGG 1 cut(s) 105
BcoDI GTCTC 1 cut(s) 713
BcuI ACTAGT 1 cut(s) 1376
BfaI CTAG 1 cut(s) 1377
BfmI CTRYAG 2 cut(s) 582, 1530
BfrI CTTAAG 1 cut(s) 1391
BglII AGATCT 1 cut(s) 858
BisI GCNGC 2 cut(s) 444, 1198
BlsI GCNGC 2 cut(s) 445, 1199
BmcAI AGTACT 1 cut(s) 1313
Bme1390I CCNGG 1 cut(s) 105
Bme18I GGWCC 3 cut(s) 228, 850, 1237
BmgT120I GGNCC 3 cut(s) 228, 850, 1237
BmiI GGNNCC 4 cut(s) 852, 1107, 1238, 1405
BmrFI CCNGG 1 cut(s) 105
BmsI GCATC 6 cut(s) 455, 632, 823, 1018, 1342, 1371
BplI GAGNNNNNCTC 2 cut(s) 1340, 1372
Bpu10I CCTNAGC 1 cut(s) 1477
Bpu14I TTCGAA 1 cut(s) 556
BsaBI GATNNNNATC 1 cut(s) 1431
BsaHI GRCGYC 1 cut(s) 796
BsaJI CCNNGG 4 cut(s) 17, 104, 1109, 1232
BsaXI ACNNNNNCTCC 2 cut(s) 186, 216
Bsc4I CCNNNNNNNGG 2 cut(s) 476, 1096
Bse21I CCTNAGG 1 cut(s) 882
Bse3DI GCAATG 2 cut(s) 935, 1185
Bse8I GATNNNNATC 1 cut(s) 1431
BseBI CCWGG 1 cut(s) 105
BseDI CCNNGG 4 cut(s) 17, 104, 1109, 1232
BseGI GGATG 5 cut(s) 442, 464, 721, 814, 1333
BseJI GATNNNNATC 1 cut(s) 1431
BseLI CCNNNNNNNGG 2 cut(s) 476, 1096
BseMI GCAATG 2 cut(s) 935, 1185
BseMII CTCAG 4 cut(s) 473, 591, 1004, 1235
BseRI GAGGAG 2 cut(s) 194, 1034
BseSI GKGCMC 1 cut(s) 543
BseXI GCAGC 1 cut(s) 1184
BsiHKAI GWGCWC 2 cut(s) 543, 924
BslFI GGGAC 2 cut(s) 836, 1250
BslI CCNNNNNNNGG 2 cut(s) 476, 1096
BsmAI GTCTC 1 cut(s) 713
BsmFI GGGAC 2 cut(s) 836, 1250
BsmI GAATGC 1 cut(s) 1351
Bsp119I TTCGAA 1 cut(s) 556
Bsp1286I GDGCHC 3 cut(s) 543, 677, 924
Bsp1407I TGTACA 1 cut(s) 1263
Bsp143I GATC 5 cut(s) 361, 858, 931, 1069, 1432
Bsp19I CCATGG 1 cut(s) 1109
BspACI CCGC 4 cut(s) 443, 470, 1039, 1118
BspCNI CTCAG 4 cut(s) 472, 592, 1003, 1236
BspLI GGNNCC 4 cut(s) 852, 1107, 1238, 1405
BspMAI CTGCAG 1 cut(s) 586
BspPI GGATC 3 cut(s) 356, 926, 1440
BspQI GCTCTTC 1 cut(s) 698
BspT104I TTCGAA 1 cut(s) 556
BspTI CTTAAG 1 cut(s) 1391
BsrDI GCAATG 2 cut(s) 935, 1185
BsrGI TGTACA 1 cut(s) 1263
BssECI CCNNGG 4 cut(s) 17, 104, 1109, 1232
BssMI GATC 5 cut(s) 361, 858, 931, 1069, 1432
BssNI GRCGYC 1 cut(s) 796
BssT1I CCWWGG 2 cut(s) 1109, 1232
Bst2UI CCWGG 1 cut(s) 105
Bst6I CTCTTC 3 cut(s) 40, 244, 698
BstACI GRCGYC 1 cut(s) 796
BstAFI CTTAAG 1 cut(s) 1391
BstAUI TGTACA 1 cut(s) 1263
BstBI TTCGAA 1 cut(s) 556
BstC8I GCNNGC 3 cut(s) 38, 71, 1552
BstDEI CTNAG 8 cut(s) 65, 74, 459, 600, 882, 990, 1244, 1477
BstDSI CCRYGG 2 cut(s) 17, 1109
BstF5I GGATG 5 cut(s) 442, 464, 721, 814, 1333
BstKTI GATC 5 cut(s) 364, 861, 934, 1072, 1435
BstMAI GTCTC 1 cut(s) 713
BstMBI GATC 5 cut(s) 361, 858, 931, 1069, 1432
BstMWI GCNNNNNNNGC 1 cut(s) 150
BstNI CCWGG 1 cut(s) 105
BstNSI RCATGY 1 cut(s) 295
BstSCI CCNGG 1 cut(s) 103
BstSFI CTRYAG 2 cut(s) 582, 1530
BstSLI GKGCMC 1 cut(s) 543
BstV1I GCAGC 1 cut(s) 1184
BstX2I RGATCY 1 cut(s) 858
BstYI RGATCY 1 cut(s) 858
Bsu36I CCTNAGG 1 cut(s) 882
BtgI CCRYGG 2 cut(s) 17, 1109
BtsCI GGATG 5 cut(s) 442, 464, 721, 814, 1333
BtsIMutI CAGTG 2 cut(s) 541, 1319
Cac8I GCNNGC 3 cut(s) 38, 71, 1552
CaiI CAGNNNCTG 1 cut(s) 125
Cfr13I GGNCC 3 cut(s) 228, 850, 1237
CseI GACGC 1 cut(s) 785
Csp6I GTAC 5 cut(s) 194, 650, 892, 1264, 1312
CviAII CATG 9 cut(s) 130, 292, 485, 532, 574, 706, 1078, 1102, 1110
CviQI GTAC 5 cut(s) 194, 650, 892, 1264, 1312
DdeI CTNAG 8 cut(s) 65, 74, 459, 600, 882, 990, 1244, 1477
DpnI GATC 5 cut(s) 363, 860, 933, 1071, 1434
DpnII GATC 5 cut(s) 361, 858, 931, 1069, 1432
Eam1104I CTCTTC 3 cut(s) 40, 244, 698
EarI CTCTTC 3 cut(s) 40, 244, 698
Ecl136II GAGCTC 1 cut(s) 922
Eco130I CCWWGG 2 cut(s) 1109, 1232
Eco24I GRGCYC 2 cut(s) 677, 924
Eco47I GGWCC 3 cut(s) 228, 850, 1237
Eco53kI GAGCTC 1 cut(s) 922
Eco57I CTGAAG 1 cut(s) 830
Eco81I CCTNAGG 1 cut(s) 882
EcoICRI GAGCTC 1 cut(s) 922
EcoO109I RGGNCCY 1 cut(s) 850
EcoRI GAATTC 1 cut(s) 345
EcoRII CCWGG 1 cut(s) 103
EcoT14I CCWWGG 2 cut(s) 1109, 1232
EcoT38I GRGCYC 2 cut(s) 677, 924
ErhI CCWWGG 2 cut(s) 1109, 1232
FaeI CATG 9 cut(s) 133, 295, 488, 535, 577, 709, 1081, 1105, 1113
FaqI GGGAC 2 cut(s) 836, 1250
FatI CATG 9 cut(s) 129, 291, 484, 531, 573, 705, 1077, 1101, 1109
FauI CCCGC 1 cut(s) 477
FblI GTMKAC 1 cut(s) 1218
Fnu4HI GCNGC 2 cut(s) 444, 1198
FokI GGATG 5 cut(s) 449, 451, 728, 801, 1320
FriOI GRGCYC 2 cut(s) 677, 924
Fsp4HI GCNGC 2 cut(s) 444, 1198
FspBI CTAG 1 cut(s) 1377
GluI GCNGC 2 cut(s) 444, 1198
HgaI GACGC 1 cut(s) 785
Hin1I GRCGYC 1 cut(s) 796
Hin1II CATG 9 cut(s) 133, 295, 488, 535, 577, 709, 1081, 1105, 1113
HincII GTYRAC 1 cut(s) 1219
HindII GTYRAC 1 cut(s) 1219
HinfI GANTC 7 cut(s) 122, 577, 1114, 1277, 1384, 1489, 1511
HphI GGTGA 1 cut(s) 581
Hpy166II GTNNAC 3 cut(s) 17, 541, 1219
Hpy188I TCNGA 2 cut(s) 451, 758
Hpy188III TCNNGA 6 cut(s) 365, 398, 728, 862, 947, 1508
Hpy8I GTNNAC 3 cut(s) 17, 541, 1219
HpyAV CCTTC 7 cut(s) 83, 103, 142, 529, 672, 831, 841
HpyCH4IV ACGT 1 cut(s) 222
HpyF10VI GCNNNNNNNGC 1 cut(s) 150
HpyF3I CTNAG 8 cut(s) 65, 74, 459, 600, 882, 990, 1244, 1477
HpySE526I ACGT 1 cut(s) 222
Hsp92I GRCGYC 1 cut(s) 796
Hsp92II CATG 9 cut(s) 133, 295, 488, 535, 577, 709, 1081, 1105, 1113
Kzo9I GATC 5 cut(s) 361, 858, 931, 1069, 1432
LguI GCTCTTC 1 cut(s) 698
LmnI GCTCC 5 cut(s) 919, 1105, 1151, 1168, 1348
Lsp1109I GCAGC 1 cut(s) 1184
LweI GCATC 6 cut(s) 455, 632, 823, 1018, 1342, 1371
MaeI CTAG 1 cut(s) 1377
MaeII ACGT 1 cut(s) 222
MaeIII GTNAC 7 cut(s) 294, 495, 569, 748, 1184, 1372, 1397
MalI GATC 5 cut(s) 363, 860, 933, 1071, 1434
MboI GATC 5 cut(s) 361, 858, 931, 1069, 1432
MboII GAAGA 8 cut(s) 57, 145, 231, 685, 982, 1064, 1093, 1135
MflI RGATCY 1 cut(s) 858
MhlI GDGCHC 3 cut(s) 543, 677, 924
MluCI AATT 4 cut(s) 198, 345, 667, 731
MlyI GAGTC 3 cut(s) 1123, 1393, 1483
MmeI TCCRAC 1 cut(s) 1361
MseI TTAA 2 cut(s) 1392, 1410
MslI CAYNNNNRTG 2 cut(s) 536, 710
MspCI CTTAAG 1 cut(s) 1391
MspR9I CCNGG 1 cut(s) 105
Mva1269I GAATGC 1 cut(s) 1351
MvaI CCWGG 1 cut(s) 105
MwoI GCNNNNNNNGC 1 cut(s) 150
NcoI CCATGG 1 cut(s) 1109
NdeII GATC 5 cut(s) 361, 858, 931, 1069, 1432
NlaIII CATG 9 cut(s) 133, 295, 488, 535, 577, 709, 1081, 1105, 1113
NlaIV GGNNCC 4 cut(s) 852, 1107, 1238, 1405
NmuCI GTSAC 2 cut(s) 495, 569
NspI RCATGY 1 cut(s) 295
NspV TTCGAA 1 cut(s) 556
PciI ACATGT 1 cut(s) 291
PciSI GCTCTTC 1 cut(s) 698
PcsI WCGNNNNNNNCGW 2 cut(s) 40, 1054
PctI GAATGC 1 cut(s) 1351
PfeI GAWTC 4 cut(s) 122, 577, 1277, 1511
PkrI GCNGC 2 cut(s) 445, 1199
PleI GAGTC 3 cut(s) 1122, 1392, 1483
PpsI GAGTC 3 cut(s) 1122, 1392, 1483
PpuMI RGGWCCY 1 cut(s) 850
PscI ACATGT 1 cut(s) 291
PsiI TTATAA 1 cut(s) 737
Psp124BI GAGCTC 1 cut(s) 924
Psp5II RGGWCCY 1 cut(s) 850
Psp6I CCWGG 1 cut(s) 103
PspGI CCWGG 1 cut(s) 103
PspN4I GGNNCC 4 cut(s) 852, 1107, 1238, 1405
PspPI GGNCC 3 cut(s) 228, 850, 1237
PspPPI RGGWCCY 1 cut(s) 850
PstI CTGCAG 1 cut(s) 586
PstNI CAGNNNCTG 1 cut(s) 125
PsuI RGATCY 1 cut(s) 858
RsaI GTAC 5 cut(s) 195, 651, 893, 1265, 1313
RsaNI GTAC 5 cut(s) 194, 650, 892, 1264, 1312
RseI CAYNNNNRTG 2 cut(s) 536, 710
SacI GAGCTC 1 cut(s) 924
SalI GTCGAC 1 cut(s) 1217
SapI GCTCTTC 1 cut(s) 698
SaqAI TTAA 2 cut(s) 1392, 1410
SatI GCNGC 2 cut(s) 444, 1198
Sau3AI GATC 5 cut(s) 361, 858, 931, 1069, 1432
Sau96I GGNCC 3 cut(s) 228, 850, 1237
ScaI AGTACT 1 cut(s) 1313
SchI GAGTC 3 cut(s) 1123, 1393, 1483
ScrFI CCNGG 1 cut(s) 105
SduI GDGCHC 3 cut(s) 543, 677, 924
SfaNI GCATC 6 cut(s) 455, 632, 823, 1018, 1342, 1371
SfcI CTRYAG 2 cut(s) 582, 1530
SfuI TTCGAA 1 cut(s) 556
SinI GGWCC 3 cut(s) 228, 850, 1237
SmiMI CAYNNNNRTG 2 cut(s) 536, 710
SmlI CTYRAG 1 cut(s) 1391
SmoI CTYRAG 1 cut(s) 1391
SpeI ACTAGT 1 cut(s) 1376
Sse9I AATT 4 cut(s) 198, 345, 667, 731
SsiI CCGC 4 cut(s) 443, 470, 1039, 1118
SspMI CTAG 1 cut(s) 1377
SstI GAGCTC 1 cut(s) 924
StyD4I CCNGG 1 cut(s) 103
StyI CCWWGG 2 cut(s) 1109, 1232
TaiI ACGT 1 cut(s) 225
TaqI TCGA 6 cut(s) 160, 343, 556, 1057, 1218, 1365
TasI AATT 4 cut(s) 198, 345, 667, 731
TatI WGTACW 3 cut(s) 891, 1263, 1311
TauI GCSGC 1 cut(s) 446
TfiI GAWTC 4 cut(s) 122, 577, 1277, 1511
Tru1I TTAA 2 cut(s) 1392, 1410
Tru9I TTAA 2 cut(s) 1392, 1410
TscAI CASTG 2 cut(s) 548, 1326
TseFI GTSAC 2 cut(s) 495, 569
TseI GCWGC 1 cut(s) 1197
Tsp45I GTSAC 2 cut(s) 495, 569
TspDTI ATGAA 8 cut(s) 146, 417, 501, 541, 548, 694, 843, 1094
TspGWI ACGGA 1 cut(s) 1416
TspRI CASTG 2 cut(s) 548, 1326
Vha464I CTTAAG 1 cut(s) 1391
VneI GTGCAC 1 cut(s) 539
VpaK11BI GGWCC 3 cut(s) 228, 850, 1237
XapI RAATTY 3 cut(s) 345, 667, 731
XceI RCATGY 1 cut(s) 295
XcmI CCANNNNNNNNNTGG 1 cut(s) 1247
XmiI GTMKAC 1 cut(s) 1218
XspI CTAG 1 cut(s) 1377
ZrmI AGTACT 1 cut(s) 1313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.