MD05G1124800.v1.1

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0006752
Physical Location & Seq
Reverse (-)
24689797 .. 24693957
4161 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1124800.v1.1.491

Sequence Viewer

Length: 1557 bp
ATGGATAAAGAGAGTCCGCGGTCTGTATCTTTTCGCACACATCGTAGAGGCGAAAGGAGTAATTTAAACCAACAGAGTAAAGATGTTGAAGAGGGATTTCCTCTGGGAAGAGTGCCGCAGGATTATCCCATGAAGATTGTTTGGAAGAAGGGCTTTATTCGATTGGTTCTTGTAGGTGGCATTTTGTGGATGCTGCTAATTCTCCTCGCGTCATTATTTCATGTTTGGTCCTGCCAGTCTTCTATCTCCTTCTTCTCAGCTATGTGTAACAAAGAGAGCAAGGTCTTCTCCATGTTAGGCTCCCTGGGGTTTGTACCAAAAGGACAAAATCAACACCGCTGCCCAATTCCTGTTGTCGGTGATCCTGATAAGATAAAAATTCCGAAGGGAGGAACTCCTGATGAAATTGTCAAAAACTTAACCTATGTTTTGGAAGATGAGATTTTGCCCAAAGGAACTCAGTCATCCCCACTATTCGGAGGACATCAAAATTGGGCACAGAGAGAGGAGAGTTTTAGACTAAAATCAGACATGAAGGTGCACTGTGGTTTTATTCGAAACGGTGGTGCTGAAATGGCTCCTGCAGACATCAAATATGCCAAGAAATGTAGGTTTGTGGTTGCATCAGGCATTTTTGATGGTTATGATGTACCCCATCAACCTTCAGATTTAAGTCCTCGTTCTAAGAAGCTCTTCTGTTTTCTCATGGCGGTCGATGAAACATCTCTTAATTTTATCAAGGAAAATGTCACTGTCAGAGAGGATAATGATGGGGGAAAATGGGTTGGTATCTGGCGTCTTGTTTTGTTGAAGCATCCACCATATGATGAACCAAGAAGGAATGGGAAGGTCCCCAAGATATTAACCCACCGCTTGTACCCTAACGCACAGTATAGCATTTGGATCGATGGTAAAATGGAGCTCATGGTTGATCCACTGCAAATTTTAGAAAGATATTTATGGCGTGGGAAGCATACATTTGCCATTTCTCAGCACAAGCACCATCACAGTATATACGAGGAGGCTGATGCAAACAAGCGGAGGAAGCGATATGCTCGACCTCTTATTGATCTACACATGAAAATTTATCGTTATGAGGGTTTGGAGTCATGGGATCCAACCAAGAAAACCGTTAGTGATGTACCAGAGGGTGCCATTATCATACGGGAACATACTGCGATGAGTAATTTGTTTAGCTGCTTGTGGTTCAATGAGGTCCACCTCTTCACACCAAGAGACCAACTGAGTTTTGGTTATGTTGTGTACCGGTTAGGGGGTTTGTTTAAGTTCTTTATGTTTCCGAATTGCGAGTACAATTCCCTGTTTGTGTTGCATCCGCACATAAGGGAGCATTCTTCTCGTATAGAGTGGGTAACAAGTTGGAATCAGCTCAAGGGGAAAGGAACTATTTCGAATAGGGGTAAAGATTGGGACCAGGCCAATGCAAAAAATGGTTTGATAGAAAGTAGGGGAGGCTTAGGCTTGTGGACTCCTTACCCTGCAAATCTTGATTCAGTTGTCCTACCCCCTGTGGCAAGAACGTCAAAAGCTGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616

Protein Analysis

519

Amino Acids

59.44

Weight (kDa)

9.41

Isoelectric Point (pI)

40.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOD1_MUCI70 PF04765 140 - 447 1.4e-129 TOD1/MUCI70, glycosyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1151
AccII CGCG 2 cut(s) 19, 209
AciI CCGC 8 cut(s) 17, 19, 116, 337, 710, 871, 1039, 1337
AclWI GGATC 5 cut(s) 356, 911, 926, 1109, 1122
AcsI RAATTY 3 cut(s) 378, 942, 1083
AcuI CTGAAG 1 cut(s) 648
AcyI GRCGYC 1 cut(s) 796
AfaI GTAC 6 cut(s) 315, 651, 878, 1143, 1265, 1313
AfiI CCNNNNNNNGG 4 cut(s) 356, 389, 476, 1273
AgeI ACCGGT 1 cut(s) 1266
AgsI TTSAA 3 cut(s) 89, 811, 1210
AjnI CCWGG 2 cut(s) 303, 1434
AluBI AGCT 6 cut(s) 260, 691, 922, 1197, 1390, 1550
AluI AGCT 6 cut(s) 260, 691, 922, 1197, 1390, 1550
Alw21I GWGCWC 2 cut(s) 543, 924
Alw26I GTCTC 1 cut(s) 1230
Alw44I GTGCAC 1 cut(s) 539
AlwI GGATC 5 cut(s) 356, 911, 926, 1109, 1122
AoxI GGCC 1 cut(s) 1437
ApaLI GTGCAC 1 cut(s) 539
ApeKI GCWGC 3 cut(s) 193, 339, 1197
ApoI RAATTY 3 cut(s) 378, 942, 1083
AsiGI ACCGGT 1 cut(s) 1266
Asp700I GAANNNNTTC 2 cut(s) 692, 1408
AspS9I GGNCC 4 cut(s) 228, 850, 1216, 1432
AsuHPI GGTGA 1 cut(s) 371
AsuII TTCGAA 2 cut(s) 556, 1412
AvaII GGWCC 4 cut(s) 228, 850, 1216, 1432
BaeGI GKGCMC 2 cut(s) 499, 543
BamHI GGATCC 1 cut(s) 1114
BanI GGYRCC 1 cut(s) 1151
BanII GRGCYC 1 cut(s) 924
BbsI GAAGAC 2 cut(s) 231, 277
Bbv12I GWGCWC 2 cut(s) 543, 924
BbvI GCAGC 3 cut(s) 180, 326, 1184
BccI CCATC 5 cut(s) 632, 663, 764, 902, 1011
BcgI CGANNNNNNTGC 4 cut(s) 886, 920, 1340, 1374
BciT130I CCWGG 2 cut(s) 305, 1436
BcoDI GTCTC 1 cut(s) 1230
BfmI CTRYAG 1 cut(s) 582
BisI GCNGC 4 cut(s) 116, 194, 340, 1198
BlsI GCNGC 4 cut(s) 117, 195, 341, 1199
Bme1390I CCNGG 2 cut(s) 305, 1436
Bme18I GGWCC 4 cut(s) 228, 850, 1216, 1432
BmgT120I GGNCC 4 cut(s) 228, 850, 1216, 1432
BmiI GGNNCC 6 cut(s) 301, 579, 852, 1116, 1153, 1433
BmrFI CCNGG 2 cut(s) 305, 1436
BmsI GCATC 5 cut(s) 180, 632, 823, 1018, 1342
BpiI GAAGAC 2 cut(s) 231, 277
Bpu10I CCTNAGC 1 cut(s) 1477
Bpu14I TTCGAA 2 cut(s) 556, 1412
BpuEI CTTGAG 1 cut(s) 1376
Bsa29I ATCGAT 1 cut(s) 906
BsaHI GRCGYC 1 cut(s) 796
BsaI GGTCTC 1 cut(s) 1230
BsaJI CCNNGG 3 cut(s) 17, 303, 304
BsaWI WCCGGW 1 cut(s) 1266
Bsc4I CCNNNNNNNGG 4 cut(s) 356, 389, 476, 1273
Bse118I RCCGGY 1 cut(s) 1266
Bse1I ACTGG 1 cut(s) 235
BseBI CCWGG 2 cut(s) 305, 1436
BseCI ATCGAT 1 cut(s) 906
BseDI CCNNGG 3 cut(s) 17, 303, 304
BseGI GGATG 4 cut(s) 195, 464, 814, 1333
BseLI CCNNNNNNNGG 4 cut(s) 356, 389, 476, 1273
BseMII CTCAG 4 cut(s) 270, 473, 1004, 1235
BseNI ACTGG 1 cut(s) 235
BseRI GAGGAG 3 cut(s) 194, 521, 1034
BseSI GKGCMC 2 cut(s) 499, 543
BseXI GCAGC 3 cut(s) 180, 326, 1184
Bsh1236I CGCG 2 cut(s) 19, 209
Bsh1285I CGRYCG 1 cut(s) 714
BshFI GGCC 1 cut(s) 1439
BshNI GGYRCC 1 cut(s) 1151
BshTI ACCGGT 1 cut(s) 1266
BshVI ATCGAT 1 cut(s) 906
BsiEI CGRYCG 1 cut(s) 714
BsiHKAI GWGCWC 2 cut(s) 543, 924
BsiSI CCGG 1 cut(s) 1267
BslFI GGGAC 2 cut(s) 836, 1445
BslI CCNNNNNNNGG 4 cut(s) 356, 389, 476, 1273
BsmAI GTCTC 1 cut(s) 1230
BsmFI GGGAC 2 cut(s) 836, 1445
BsmI GAATGC 1 cut(s) 1351
BsnI GGCC 1 cut(s) 1439
Bso31I GGTCTC 1 cut(s) 1230
Bsp119I TTCGAA 2 cut(s) 556, 1412
Bsp1286I GDGCHC 3 cut(s) 499, 543, 924
Bsp143I GATC 5 cut(s) 361, 903, 931, 1069, 1114
BspACI CCGC 8 cut(s) 17, 19, 116, 337, 710, 871, 1039, 1337
BspANI GGCC 1 cut(s) 1439
BspCNI CTCAG 4 cut(s) 269, 472, 1003, 1236
BspDI ATCGAT 1 cut(s) 906
BspFNI CGCG 2 cut(s) 19, 209
BspLI GGNNCC 6 cut(s) 301, 579, 852, 1116, 1153, 1433
BspMAI CTGCAG 1 cut(s) 586
BspPI GGATC 5 cut(s) 356, 911, 926, 1109, 1122
BspQI GCTCTTC 1 cut(s) 698
BspT104I TTCGAA 2 cut(s) 556, 1412
BspT107I GGYRCC 1 cut(s) 1151
BspTNI GGTCTC 1 cut(s) 1230
BsrFI RCCGGY 1 cut(s) 1266
BsrI ACTGG 1 cut(s) 235
BssAI RCCGGY 1 cut(s) 1266
BssECI CCNNGG 3 cut(s) 17, 303, 304
BssMI GATC 5 cut(s) 361, 903, 931, 1069, 1114
BssNI GRCGYC 1 cut(s) 796
Bst2UI CCWGG 2 cut(s) 305, 1436
Bst4CI ACNGT 6 cut(s) 545, 563, 754, 891, 1010, 1132
Bst6I CTCTTC 4 cut(s) 84, 103, 698, 1229
BstACI GRCGYC 1 cut(s) 796
BstBI TTCGAA 2 cut(s) 556, 1412
BstC8I GCNNGC 1 cut(s) 1552
BstDEI CTNAG 6 cut(s) 256, 459, 684, 990, 1244, 1477
BstDSI CCRYGG 1 cut(s) 17
BstF5I GGATG 4 cut(s) 195, 464, 814, 1333
BstFNI CGCG 2 cut(s) 19, 209
BstKTI GATC 5 cut(s) 364, 906, 934, 1072, 1117
BstMAI GTCTC 1 cut(s) 1230
BstMBI GATC 5 cut(s) 361, 903, 931, 1069, 1114
BstMCI CGRYCG 1 cut(s) 714
BstMWI GCNNNNNNNGC 3 cut(s) 575, 970, 1045
BstNI CCWGG 2 cut(s) 305, 1436
BstSCI CCNGG 2 cut(s) 303, 1434
BstSFI CTRYAG 1 cut(s) 582
BstSLI GKGCMC 2 cut(s) 499, 543
BstUI CGCG 2 cut(s) 19, 209
BstV1I GCAGC 3 cut(s) 180, 326, 1184
BstV2I GAAGAC 2 cut(s) 231, 277
BstX2I RGATCY 1 cut(s) 1114
BstYI RGATCY 1 cut(s) 1114
Bsu15I ATCGAT 1 cut(s) 906
BsuRI GGCC 1 cut(s) 1439
BsuTUI ATCGAT 1 cut(s) 906
BtgI CCRYGG 1 cut(s) 17
BtgZI GCGATG 1 cut(s) 1193
BtsCI GGATG 4 cut(s) 195, 464, 814, 1333
BtsI GCAGTG 1 cut(s) 935
BtsIMutI CAGTG 3 cut(s) 541, 750, 935
Cac8I GCNNGC 1 cut(s) 1552
Cfr10I RCCGGY 1 cut(s) 1266
Cfr13I GGNCC 4 cut(s) 228, 850, 1216, 1432
Cfr42I CCGCGG 1 cut(s) 20
ClaI ATCGAT 1 cut(s) 906
CseI GACGC 2 cut(s) 198, 785
Csp6I GTAC 6 cut(s) 314, 650, 877, 1142, 1264, 1312
CspAI ACCGGT 1 cut(s) 1266
CviAII CATG 8 cut(s) 130, 221, 292, 532, 706, 925, 1078, 1110
CviQI GTAC 6 cut(s) 314, 650, 877, 1142, 1264, 1312
DdeI CTNAG 6 cut(s) 256, 459, 684, 990, 1244, 1477
DpnI GATC 5 cut(s) 363, 905, 933, 1071, 1116
DpnII GATC 5 cut(s) 361, 903, 931, 1069, 1114
DraI TTTAAA 1 cut(s) 66
Eam1104I CTCTTC 4 cut(s) 84, 103, 698, 1229
EarI CTCTTC 4 cut(s) 84, 103, 698, 1229
Ecl136II GAGCTC 1 cut(s) 922
Eco24I GRGCYC 1 cut(s) 924
Eco31I GGTCTC 1 cut(s) 1230
Eco47I GGWCC 4 cut(s) 228, 850, 1216, 1432
Eco53kI GAGCTC 1 cut(s) 922
Eco57I CTGAAG 1 cut(s) 648
EcoICRI GAGCTC 1 cut(s) 922
EcoO109I RGGNCCY 1 cut(s) 850
EcoRII CCWGG 2 cut(s) 303, 1434
EcoT38I GRGCYC 1 cut(s) 924
FaeI CATG 8 cut(s) 133, 224, 295, 535, 709, 928, 1081, 1113
FalI AAGNNNNNCTT 4 cut(s) 137, 169, 677, 709
FaqI GGGAC 2 cut(s) 836, 1445
FatI CATG 8 cut(s) 129, 220, 291, 531, 705, 924, 1077, 1109
FauNDI CATATG 1 cut(s) 823
Fnu4HI GCNGC 4 cut(s) 116, 194, 340, 1198
FokI GGATG 4 cut(s) 202, 451, 801, 1320
FriOI GRGCYC 1 cut(s) 924
Fsp4HI GCNGC 4 cut(s) 116, 194, 340, 1198
GluI GCNGC 4 cut(s) 116, 194, 340, 1198
HaeIII GGCC 1 cut(s) 1439
HapII CCGG 1 cut(s) 1267
HgaI GACGC 2 cut(s) 198, 785
Hin1I GRCGYC 1 cut(s) 796
Hin1II CATG 8 cut(s) 133, 224, 295, 535, 709, 928, 1081, 1113
HinfI GANTC 5 cut(s) 13, 1106, 1384, 1489, 1511
HpaII CCGG 1 cut(s) 1267
HphI GGTGA 1 cut(s) 371
Hpy166II GTNNAC 4 cut(s) 541, 1219, 1264, 1488
Hpy188I TCNGA 6 cut(s) 384, 479, 529, 667, 758, 1302
Hpy188III TCNNGA 3 cut(s) 365, 398, 1508
Hpy8I GTNNAC 4 cut(s) 541, 1219, 1264, 1488
HpyAV CCTTC 7 cut(s) 142, 259, 379, 529, 672, 831, 841
HpyCH4III ACNGT 6 cut(s) 545, 563, 754, 891, 1010, 1132
HpyCH4IV ACGT 1 cut(s) 1541
HpyCH4V TGCA 8 cut(s) 541, 584, 623, 940, 1031, 1333, 1445, 1502
HpyF10VI GCNNNNNNNGC 3 cut(s) 575, 970, 1045
HpyF3I CTNAG 6 cut(s) 256, 459, 684, 990, 1244, 1477
HpySE526I ACGT 1 cut(s) 1541
Hsp92I GRCGYC 1 cut(s) 796
Hsp92II CATG 8 cut(s) 133, 224, 295, 535, 709, 928, 1081, 1113
KspI CCGCGG 1 cut(s) 20
Kzo9I GATC 5 cut(s) 361, 903, 931, 1069, 1114
LguI GCTCTTC 1 cut(s) 698
LmnI GCTCC 4 cut(s) 305, 583, 919, 1348
Lsp1109I GCAGC 3 cut(s) 180, 326, 1184
LweI GCATC 5 cut(s) 180, 632, 823, 1018, 1342
MaeII ACGT 1 cut(s) 1541
MaeIII GTNAC 3 cut(s) 266, 748, 1372
MalI GATC 5 cut(s) 363, 905, 933, 1071, 1116
MboI GATC 5 cut(s) 361, 903, 931, 1069, 1114
MflI RGATCY 1 cut(s) 1114
MhlI GDGCHC 3 cut(s) 499, 543, 924
MlyI GAGTC 3 cut(s) 22, 1115, 1483
MmeI TCCRAC 2 cut(s) 1142, 1361
MroXI GAANNNNTTC 2 cut(s) 692, 1408
MseI TTAA 6 cut(s) 65, 419, 671, 729, 863, 1284
MslI CAYNNNNRTG 1 cut(s) 536
MspA1I CMGCKG 2 cut(s) 19, 339
MspI CCGG 1 cut(s) 1267
MspR9I CCNGG 2 cut(s) 305, 1436
Mva1269I GAATGC 1 cut(s) 1351
MvaI CCWGG 2 cut(s) 305, 1436
MvnI CGCG 2 cut(s) 19, 209
MwoI GCNNNNNNNGC 3 cut(s) 575, 970, 1045
NdeI CATATG 1 cut(s) 823
NdeII GATC 5 cut(s) 361, 903, 931, 1069, 1114
NlaIII CATG 8 cut(s) 133, 224, 295, 535, 709, 928, 1081, 1113
NlaIV GGNNCC 6 cut(s) 301, 579, 852, 1116, 1153, 1433
NmuCI GTSAC 1 cut(s) 748
NspV TTCGAA 2 cut(s) 556, 1412
PasI CCCWGGG 1 cut(s) 304
PciSI GCTCTTC 1 cut(s) 698
PcsI WCGNNNNNNNCGW 1 cut(s) 40
PctI GAATGC 1 cut(s) 1351
PdmI GAANNNNTTC 2 cut(s) 692, 1408
PfeI GAWTC 2 cut(s) 1384, 1511
PinAI ACCGGT 1 cut(s) 1266
PkrI GCNGC 4 cut(s) 117, 195, 341, 1199
PleI GAGTC 3 cut(s) 21, 1114, 1483
PpsI GAGTC 3 cut(s) 21, 1114, 1483
PpuMI RGGWCCY 1 cut(s) 850
Psp124BI GAGCTC 1 cut(s) 924
Psp5II RGGWCCY 1 cut(s) 850
Psp6I CCWGG 2 cut(s) 303, 1434
PspGI CCWGG 2 cut(s) 303, 1434
PspN4I GGNNCC 6 cut(s) 301, 579, 852, 1116, 1153, 1433
PspPI GGNCC 4 cut(s) 228, 850, 1216, 1432
PspPPI RGGWCCY 1 cut(s) 850
PstI CTGCAG 1 cut(s) 586
PsuI RGATCY 1 cut(s) 1114
RsaI GTAC 6 cut(s) 315, 651, 878, 1143, 1265, 1313
RsaNI GTAC 6 cut(s) 314, 650, 877, 1142, 1264, 1312
RseI CAYNNNNRTG 1 cut(s) 536
SacI GAGCTC 1 cut(s) 924
SacII CCGCGG 1 cut(s) 20
SapI GCTCTTC 1 cut(s) 698
SaqAI TTAA 6 cut(s) 65, 419, 671, 729, 863, 1284
SatI GCNGC 4 cut(s) 116, 194, 340, 1198
Sau3AI GATC 5 cut(s) 361, 903, 931, 1069, 1114
Sau96I GGNCC 4 cut(s) 228, 850, 1216, 1432
SchI GAGTC 3 cut(s) 22, 1115, 1483
ScrFI CCNGG 2 cut(s) 305, 1436
SduI GDGCHC 3 cut(s) 499, 543, 924
SfaNI GCATC 5 cut(s) 180, 632, 823, 1018, 1342
SfcI CTRYAG 1 cut(s) 582
Sfr303I CCGCGG 1 cut(s) 20
SfuI TTCGAA 2 cut(s) 556, 1412
SgrBI CCGCGG 1 cut(s) 20
SinI GGWCC 4 cut(s) 228, 850, 1216, 1432
SmiMI CAYNNNNRTG 1 cut(s) 536
SmlI CTYRAG 1 cut(s) 1391
SmoI CTYRAG 1 cut(s) 1391
SsiI CCGC 8 cut(s) 17, 19, 116, 337, 710, 871, 1039, 1337
SstI GAGCTC 1 cut(s) 924
StyD4I CCNGG 2 cut(s) 303, 1434
TaaI ACNGT 6 cut(s) 545, 563, 754, 891, 1010, 1132
TaiI ACGT 1 cut(s) 1544
TaqI TCGA 6 cut(s) 160, 556, 714, 906, 1057, 1412
TatI WGTACW 1 cut(s) 1311
TauI GCSGC 1 cut(s) 118
TfiI GAWTC 2 cut(s) 1384, 1511
Tru1I TTAA 6 cut(s) 65, 419, 671, 729, 863, 1284
Tru9I TTAA 6 cut(s) 65, 419, 671, 729, 863, 1284
TscAI CASTG 3 cut(s) 548, 757, 942
TseFI GTSAC 1 cut(s) 748
TseI GCWGC 3 cut(s) 193, 339, 1197
Tsp45I GTSAC 1 cut(s) 748
TspDTI ATGAA 7 cut(s) 146, 209, 417, 548, 732, 843, 1094
TspRI CASTG 3 cut(s) 548, 757, 942
VneI GTGCAC 1 cut(s) 539
VpaK11BI GGWCC 4 cut(s) 228, 850, 1216, 1432
XapI RAATTY 3 cut(s) 378, 942, 1083
XcmI CCANNNNNNNNNTGG 1 cut(s) 1247
XmnI GAANNNNTTC 2 cut(s) 692, 1408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.