Rorug06G0119400

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0006752
Physical Location & Seq
Reverse (-)
16347163 .. 16349787
2625 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0119400.1

Sequence Viewer

Length: 1800 bp
ATGGGCGTTCGATTCATCATCAGCTTCTGCCTTCTGTTAGTAGTGTGTCGTGAGGCCAATGGAGCAAGGAATGTACTCAAACAGGAGCCAAAAGAAGAAGATGCACCATACATTACTAGTTATGGAATCGAAGAAGGCATAAAAGAGGTCGAAGCTCCTTACATAACTCAGTATGGTACAAAAGAAGGCAAAGTGGAAGAGGTCGAAGCTCCTTACATAACTCAGTATGGTACAGAAGAAGGCAAAGTGGAAGAGGTCGAAGCTCCTTACATAACCGGGTACATTTCTGCGGAAGGCAACACCAAGGAAGTAGAGACGCCATACATAACAGGATATATTTCCGCACAAGACACGAAGGGCACAAAGGAAGTAGAGGCGCCTTACATCACTGGATACATTTCCAAACGAGGCACAAAGAGCACAAAAGATGTAGAGGCACCTTATATAACACAATATCAAAACAAAGAGGCTAATTTCTACAATACCCAGTATGGAACGAAAGAAGGAAAGAATAAGGTAGAGGAGCCTTACCTAACTGGTTACAAGACCACAAGGGAGTCAAAAGAGGTAGAAGCGCCTTACATAACAGGGTACATTTCCTCACAAGGCACAAAGCTCAAGAAGGAGGTAGAGGCGCCTTACATAACCGGATACATTTCTGCACAAGGCAGCAAGCTCAAGAAAGAGGTAGAGGCACCTTACATAACCGGATACATTTCTGCACAAGGCACAAATGGCAAGGAGGTAGAGGCGCCTTACATAACTGGATACATTTCCGCACAAGGCACAAAGCTCAAGAAGGAGGTAGAGGCGCCTTACATCACCGGATATATTTCCGCACAAGGAACAAAGGGCAAGAAAGAGGTAGAGGCGCCTTATATAACTGGATATATTTCCGCACAAGGCACAAAGGGCAAGAAGGAAGTAGAGGCGCCTTATATAACAGGATATATTTCTGCACAAGGCGCAAAAAACTGCGAACATCAGAAGCATGTACATCCACCTTCCCAAAATGGAGCAAAGGATGTGGTTTCGGGTAAGGAGTTACCTATGGACCATCAACATCATGATCATATGCATGCACATTCATCATCTCCCCTGGATCATACTGAGGCACTCAAGCAAGGATTTTTTACTTTTGAGGATTTCTACAAAGGGAATGCACTGCCACTCAACTTCCCCAAACAAGACCATTCTCGTTTCTTGCCTAAAGCAACAGCCGATTCCATTCCATTTTCAACATCTCAGCTCCCACACCTTCTCCAACTCTTCTCAACCCCACGCGACTCTCGTGACGCAAAACACATGGCCTGGACACTCGGCCAGTGCGAACTGAAACCCATTAAGGGGGAGACGAAGTTCTGTGCTACTTCTCTAGAATCCATGATAGATTTTGTTCAAAAAATCATTGGTTCAGGGGTCAACTTCAATATTCTCTCTACCACCCACCCAGAAACCTCAACTGCCATCACACAAAAGTACACTATCTTGGATGAGCCCAAACAAGTCTTAGCTTCCAAAATGGTCTTCTGCCACCCGGTCCCTTATCCTTATGCTGTTTTCTTCTGCCACCACTTTGAGAACGATACCAAGTTCTTCAAGGTTTCACTGGGAGGCGAGAACGGAGATAAGGTGGAAGCCGTAGCTGTTTGCCACATGGACACTTCTGATTGGGACCCTAACCACAGTTTGTTTCGCTTATTGGGAATCAAGGCTGGTGCTTCTTCACCAGTGTGCCATTTCTTCCCTGGAAATCATCTAGTTTGGATTCCATCATCACCAACAACAGCTACCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616

Protein Analysis

599

Amino Acids

66.46

Weight (kDa)

6.12

Isoelectric Point (pI)

25.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BURP PF03181 377 - 590 2.8e-74 BURP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 8 cut(s) 376, 436, 634, 694, 751, 811, 871, 931
AccII CGCG 1 cut(s) 1284
AciI CCGC 5 cut(s) 290, 342, 777, 837, 897
AclWI GGATC 1 cut(s) 1110
AcoI YGGCCR 1 cut(s) 1321
AcyI GRCGYC 7 cut(s) 317, 377, 635, 752, 812, 872, 932
AfaI GTAC 7 cut(s) 75, 178, 232, 281, 593, 996, 1481
AfiI CCNNNNNNNGG 2 cut(s) 1346, 1347
AgsI TTSAA 4 cut(s) 1239, 1400, 1429, 1600
AhlI ACTAGT 1 cut(s) 116
AjnI CCWGG 3 cut(s) 1098, 1310, 1747
AjuI GAANNNNNNNTTGG 2 cut(s) 1511, 1543
AleI CACNNNNGTG 1 cut(s) 1732
AloI GAACNNNNNNTCC 2 cut(s) 1343, 1375
Alw21I GWGCWC 1 cut(s) 422
Alw26I GTCTC 2 cut(s) 308, 1346
AlwI GGATC 1 cut(s) 1110
AlwNI CAGNNNCTG 1 cut(s) 27
AoxI GGCC 3 cut(s) 54, 1308, 1321
ApeKI GCWGC 1 cut(s) 669
AspLEI GCGC 8 cut(s) 379, 577, 637, 754, 814, 874, 934, 968
AspS9I GGNCC 3 cut(s) 1054, 1540, 1675
AsuC2I CCSGG 2 cut(s) 277, 1538
AsuHPI GGTGA 3 cut(s) 814, 1719, 1770
AvaII GGWCC 3 cut(s) 1054, 1540, 1675
BaeGI GKGCMC 1 cut(s) 362
BanI GGYRCC 8 cut(s) 376, 436, 634, 694, 751, 811, 871, 931
BanII GRGCYC 1 cut(s) 1500
BauI CACGAG 1 cut(s) 1290
BbsI GAAGAC 1 cut(s) 1519
Bbv12I GWGCWC 1 cut(s) 422
BbvI GCAGC 1 cut(s) 681
BccI CCATC 3 cut(s) 1065, 1475, 1780
BceAI ACGGC 1 cut(s) 1625
BciT130I CCWGG 3 cut(s) 1100, 1312, 1749
BciVI GTATCC 4 cut(s) 386, 644, 704, 761
BclI TGATCA 1 cut(s) 1069
BcnI CCSGG 2 cut(s) 277, 1538
BcoDI GTCTC 2 cut(s) 308, 1346
BcuI ACTAGT 1 cut(s) 116
BfaI CTAG 3 cut(s) 117, 1376, 1760
BfoI RGCGCY 7 cut(s) 380, 578, 638, 755, 815, 875, 935
BfuI GTATCC 4 cut(s) 386, 644, 704, 761
BisI GCNGC 1 cut(s) 670
BlsI GCNGC 1 cut(s) 671
Bme1390I CCNGG 5 cut(s) 277, 1100, 1312, 1538, 1749
Bme18I GGWCC 3 cut(s) 1054, 1540, 1675
BmgT120I GGNCC 3 cut(s) 1054, 1540, 1675
BmrFI CCNGG 5 cut(s) 277, 1100, 1312, 1538, 1749
BmrI ACTGGG 2 cut(s) 481, 1619
BmsI GCATC 1 cut(s) 91
BmuI ACTGGG 2 cut(s) 481, 1619
BpiI GAAGAC 1 cut(s) 1519
BpuEI CTTGAG 4 cut(s) 602, 662, 779, 1103
BpuMI CCSGG 2 cut(s) 277, 1538
BsaHI GRCGYC 7 cut(s) 317, 377, 635, 752, 812, 872, 932
BsaJI CCNNGG 3 cut(s) 303, 1098, 1747
BsaWI WCCGGW 3 cut(s) 647, 707, 824
BsaXI ACNNNNNCTCC 6 cut(s) 1245, 1275, 1343, 1373, 1617, 1647
Bsc4I CCNNNNNNNGG 2 cut(s) 1346, 1347
Bse1I ACTGG 8 cut(s) 394, 487, 541, 769, 889, 1324, 1614, 1730
BseBI CCWGG 3 cut(s) 1100, 1312, 1749
BseDI CCNNGG 3 cut(s) 303, 1098, 1747
BseGI GGATG 3 cut(s) 997, 1030, 1498
BseLI CCNNNNNNNGG 2 cut(s) 1346, 1347
BseMII CTCAG 4 cut(s) 182, 236, 1101, 1259
BseNI ACTGG 8 cut(s) 394, 487, 541, 769, 889, 1324, 1614, 1730
BseRI GAGGAG 1 cut(s) 536
BseSI GKGCMC 1 cut(s) 362
BseXI GCAGC 1 cut(s) 681
BsgI GTGCAG 3 cut(s) 645, 705, 942
Bsh1236I CGCG 1 cut(s) 1284
BshFI GGCC 3 cut(s) 56, 1310, 1323
BshNI GGYRCC 8 cut(s) 376, 436, 634, 694, 751, 811, 871, 931
BsiHKAI GWGCWC 1 cut(s) 422
BsiSI CCGG 5 cut(s) 276, 648, 708, 825, 1538
BslFI GGGAC 2 cut(s) 1526, 1688
BslI CCNNNNNNNGG 2 cut(s) 1346, 1347
BsmAI GTCTC 2 cut(s) 308, 1346
BsmBI CGTCTC 2 cut(s) 308, 1346
BsmFI GGGAC 2 cut(s) 1526, 1688
BsmI GAATGC 1 cut(s) 1165
BsnI GGCC 3 cut(s) 56, 1310, 1323
Bsp1286I GDGCHC 3 cut(s) 362, 422, 1500
Bsp1407I TGTACA 1 cut(s) 994
Bsp143I GATC 2 cut(s) 1069, 1102
BspACI CCGC 5 cut(s) 290, 342, 777, 837, 897
BspANI GGCC 3 cut(s) 56, 1310, 1323
BspCNI CTCAG 4 cut(s) 181, 235, 1102, 1258
BspFNI CGCG 1 cut(s) 1284
BspHI TCATGA 1 cut(s) 1066
BspPI GGATC 1 cut(s) 1110
BspT107I GGYRCC 8 cut(s) 376, 436, 634, 694, 751, 811, 871, 931
BsrGI TGTACA 1 cut(s) 994
BsrI ACTGG 8 cut(s) 394, 487, 541, 769, 889, 1324, 1614, 1730
BssECI CCNNGG 3 cut(s) 303, 1098, 1747
BssMI GATC 2 cut(s) 1069, 1102
BssNI GRCGYC 7 cut(s) 317, 377, 635, 752, 812, 872, 932
BssSI CACGAG 1 cut(s) 1290
BssT1I CCWWGG 1 cut(s) 303
Bst2BI CACGAG 1 cut(s) 1290
Bst2UI CCWGG 3 cut(s) 1100, 1312, 1749
Bst4CI ACNGT 1 cut(s) 1688
Bst6I CTCTTC 3 cut(s) 192, 246, 1274
BstACI GRCGYC 7 cut(s) 317, 377, 635, 752, 812, 872, 932
BstAUI TGTACA 1 cut(s) 994
BstC8I GCNNGC 2 cut(s) 674, 1080
BstDEI CTNAG 5 cut(s) 168, 222, 1110, 1245, 1510
BstF5I GGATG 3 cut(s) 997, 1030, 1498
BstFNI CGCG 1 cut(s) 1284
BstH2I RGCGCY 7 cut(s) 380, 578, 638, 755, 815, 875, 935
BstHHI GCGC 8 cut(s) 379, 577, 637, 754, 814, 874, 934, 968
BstKTI GATC 2 cut(s) 1072, 1105
BstMAI GTCTC 2 cut(s) 308, 1346
BstMBI GATC 2 cut(s) 1069, 1102
BstMWI GCNNNNNNNGC 5 cut(s) 62, 417, 735, 912, 965
BstNI CCWGG 3 cut(s) 1100, 1312, 1749
BstNSI RCATGY 2 cut(s) 995, 1082
BstSCI CCNGG 5 cut(s) 275, 1098, 1310, 1536, 1747
BstSLI GKGCMC 1 cut(s) 362
BstUI CGCG 1 cut(s) 1284
BstV1I GCAGC 1 cut(s) 681
BstV2I GAAGAC 1 cut(s) 1519
BsuI GTATCC 4 cut(s) 386, 644, 704, 761
BsuRI GGCC 3 cut(s) 56, 1310, 1323
BtsCI GGATG 3 cut(s) 997, 1030, 1498
BtsI GCAGTG 1 cut(s) 1163
BtsIMutI CAGTG 5 cut(s) 387, 1163, 1331, 1607, 1737
Cac8I GCNNGC 2 cut(s) 674, 1080
CaiI CAGNNNCTG 1 cut(s) 27
CciI TCATGA 1 cut(s) 1066
CfoI GCGC 8 cut(s) 379, 577, 637, 754, 814, 874, 934, 968
Cfr13I GGNCC 3 cut(s) 1054, 1540, 1675
CseI GACGC 2 cut(s) 325, 1304
Csp6I GTAC 7 cut(s) 74, 177, 231, 280, 592, 995, 1480
CspCI CAANNNNNGTGG 2 cut(s) 1008, 1043
CviAII CATG 6 cut(s) 992, 1067, 1079, 1306, 1384, 1657
CviQI GTAC 7 cut(s) 74, 177, 231, 280, 592, 995, 1480
DdeI CTNAG 5 cut(s) 168, 222, 1110, 1245, 1510
DinI GGCGCC 6 cut(s) 378, 636, 753, 813, 873, 933
DpnI GATC 2 cut(s) 1071, 1104
DpnII GATC 2 cut(s) 1069, 1102
EaeI YGGCCR 1 cut(s) 1321
Eam1104I CTCTTC 3 cut(s) 192, 246, 1274
EarI CTCTTC 3 cut(s) 192, 246, 1274
Eco130I CCWWGG 1 cut(s) 303
Eco24I GRGCYC 1 cut(s) 1500
Eco47I GGWCC 3 cut(s) 1054, 1540, 1675
EcoO109I RGGNCCY 1 cut(s) 1675
EcoRII CCWGG 3 cut(s) 1098, 1310, 1747
EcoT14I CCWWGG 1 cut(s) 303
EcoT22I ATGCAT 1 cut(s) 1080
EcoT38I GRGCYC 1 cut(s) 1500
EgeI GGCGCC 6 cut(s) 378, 636, 753, 813, 873, 933
EheI GGCGCC 6 cut(s) 378, 636, 753, 813, 873, 933
ErhI CCWWGG 1 cut(s) 303
Esp3I CGTCTC 2 cut(s) 308, 1346
FaeI CATG 6 cut(s) 995, 1070, 1082, 1309, 1387, 1660
FaqI GGGAC 2 cut(s) 1526, 1688
FatI CATG 6 cut(s) 991, 1066, 1078, 1305, 1383, 1656
FauNDI CATATG 1 cut(s) 1074
FbaI TGATCA 1 cut(s) 1069
Fnu4HI GCNGC 1 cut(s) 670
FokI GGATG 3 cut(s) 984, 1037, 1505
FriOI GRGCYC 1 cut(s) 1500
Fsp4HI GCNGC 1 cut(s) 670
FspBI CTAG 3 cut(s) 117, 1376, 1760
GlaI GCGC 8 cut(s) 378, 576, 636, 753, 813, 873, 933, 967
GluI GCNGC 1 cut(s) 670
HaeII RGCGCY 7 cut(s) 380, 578, 638, 755, 815, 875, 935
HaeIII GGCC 3 cut(s) 56, 1310, 1323
HapII CCGG 5 cut(s) 276, 648, 708, 825, 1538
HgaI GACGC 2 cut(s) 325, 1304
HhaI GCGC 8 cut(s) 379, 577, 637, 754, 814, 874, 934, 968
Hin1I GRCGYC 7 cut(s) 317, 377, 635, 752, 812, 872, 932
Hin1II CATG 6 cut(s) 995, 1070, 1082, 1309, 1387, 1660
Hin6I GCGC 8 cut(s) 377, 575, 635, 752, 812, 872, 932, 966
HinP1I GCGC 8 cut(s) 377, 575, 635, 752, 812, 872, 932, 966
HincII GTYRAC 1 cut(s) 1423
HindII GTYRAC 1 cut(s) 1423
HinfI GANTC 8 cut(s) 12, 126, 557, 1223, 1286, 1379, 1707, 1768
HpaII CCGG 5 cut(s) 276, 648, 708, 825, 1538
HphI GGTGA 3 cut(s) 814, 1719, 1770
Hpy166II GTNNAC 2 cut(s) 1423, 1482
Hpy188I TCNGA 2 cut(s) 987, 1669
Hpy188III TCNNGA 7 cut(s) 50, 619, 679, 796, 1067, 1292, 1376
Hpy8I GTNNAC 2 cut(s) 1423, 1482
HpyCH4III ACNGT 1 cut(s) 1688
HpyCH4V TGCA 7 cut(s) 104, 662, 722, 959, 1078, 1082, 1163
HpyF10VI GCNNNNNNNGC 5 cut(s) 62, 417, 735, 912, 965
HpyF3I CTNAG 5 cut(s) 168, 222, 1110, 1245, 1510
Hsp92I GRCGYC 7 cut(s) 317, 377, 635, 752, 812, 872, 932
Hsp92II CATG 6 cut(s) 995, 1070, 1082, 1309, 1387, 1660
HspAI GCGC 8 cut(s) 377, 575, 635, 752, 812, 872, 932, 966
KasI GGCGCC 6 cut(s) 376, 634, 751, 811, 871, 931
KflI GGGWCCC 1 cut(s) 1675
Ksp22I TGATCA 1 cut(s) 1069
Kzo9I GATC 2 cut(s) 1069, 1102
LmnI GCTCC 8 cut(s) 62, 85, 160, 214, 268, 523, 1016, 1254
Lsp1109I GCAGC 1 cut(s) 681
LweI GCATC 1 cut(s) 91
MaeI CTAG 3 cut(s) 117, 1376, 1760
MaeIII GTNAC 3 cut(s) 539, 1044, 1292
MalI GATC 2 cut(s) 1071, 1104
MboI GATC 2 cut(s) 1069, 1102
MhlI GDGCHC 3 cut(s) 362, 422, 1500
MluCI AATT 1 cut(s) 472
Mly113I GGCGCC 6 cut(s) 377, 635, 752, 812, 872, 932
MlyI GAGTC 2 cut(s) 566, 1280
MmeI TCCRAC 1 cut(s) 1288
Mph1103I ATGCAT 1 cut(s) 1080
MseI TTAA 2 cut(s) 1344, 1798
MslI CAYNNNNRTG 2 cut(s) 1077, 1732
MspI CCGG 5 cut(s) 276, 648, 708, 825, 1538
MspR9I CCNGG 5 cut(s) 277, 1100, 1312, 1538, 1749
Mva1269I GAATGC 1 cut(s) 1165
MvaI CCWGG 3 cut(s) 1100, 1312, 1749
MvnI CGCG 1 cut(s) 1284
MwoI GCNNNNNNNGC 5 cut(s) 62, 417, 735, 912, 965
NarI GGCGCC 6 cut(s) 377, 635, 752, 812, 872, 932
NciI CCSGG 2 cut(s) 277, 1538
NdeI CATATG 1 cut(s) 1074
NdeII GATC 2 cut(s) 1069, 1102
NlaIII CATG 6 cut(s) 995, 1070, 1082, 1309, 1387, 1660
NmeAIII GCCGAG 1 cut(s) 1299
NmuCI GTSAC 1 cut(s) 1292
NsiI ATGCAT 1 cut(s) 1080
NspI RCATGY 2 cut(s) 995, 1082
OliI CACNNNNGTG 1 cut(s) 1732
PaeI GCATGC 1 cut(s) 1082
PagI TCATGA 1 cut(s) 1066
PcsI WCGNNNNNNNCGW 2 cut(s) 1288, 1326
PctI GAATGC 1 cut(s) 1165
PfeI GAWTC 6 cut(s) 12, 126, 1223, 1379, 1707, 1768
PkrI GCNGC 1 cut(s) 671
PleI GAGTC 2 cut(s) 565, 1280
PluTI GGCGCC 6 cut(s) 380, 638, 755, 815, 875, 935
PpsI GAGTC 2 cut(s) 565, 1280
PpuMI RGGWCCY 1 cut(s) 1675
Psp5II RGGWCCY 1 cut(s) 1675
Psp6I CCWGG 3 cut(s) 1098, 1310, 1747
PspGI CCWGG 3 cut(s) 1098, 1310, 1747
PspPI GGNCC 3 cut(s) 1054, 1540, 1675
PspPPI RGGWCCY 1 cut(s) 1675
PstNI CAGNNNCTG 1 cut(s) 27
RsaI GTAC 7 cut(s) 75, 178, 232, 281, 593, 996, 1481
RsaNI GTAC 7 cut(s) 74, 177, 231, 280, 592, 995, 1480
RseI CAYNNNNRTG 2 cut(s) 1077, 1732
SaqAI TTAA 2 cut(s) 1344, 1798
SatI GCNGC 1 cut(s) 670
Sau3AI GATC 2 cut(s) 1069, 1102
Sau96I GGNCC 3 cut(s) 1054, 1540, 1675
SchI GAGTC 2 cut(s) 566, 1280
ScrFI CCNGG 5 cut(s) 277, 1100, 1312, 1538, 1749
SduI GDGCHC 3 cut(s) 362, 422, 1500
SfaNI GCATC 1 cut(s) 91
SfoI GGCGCC 6 cut(s) 378, 636, 753, 813, 873, 933
SinI GGWCC 3 cut(s) 1054, 1540, 1675
SmiMI CAYNNNNRTG 2 cut(s) 1077, 1732
SmlI CTYRAG 4 cut(s) 617, 677, 794, 1118
SmoI CTYRAG 4 cut(s) 617, 677, 794, 1118
SpeI ACTAGT 1 cut(s) 116
SphI GCATGC 1 cut(s) 1082
Sse9I AATT 1 cut(s) 472
SsiI CCGC 5 cut(s) 290, 342, 777, 837, 897
SspDI GGCGCC 6 cut(s) 376, 634, 751, 811, 871, 931
SspI AATATT 1 cut(s) 1432
SspMI CTAG 3 cut(s) 117, 1376, 1760
StyD4I CCNGG 5 cut(s) 275, 1098, 1310, 1536, 1747
StyI CCWWGG 1 cut(s) 303
TaaI ACNGT 1 cut(s) 1688
TaqI TCGA 5 cut(s) 10, 129, 150, 204, 258
TasI AATT 1 cut(s) 472
TatI WGTACW 3 cut(s) 73, 994, 1479
TfiI GAWTC 6 cut(s) 12, 126, 1223, 1379, 1707, 1768
Tru1I TTAA 2 cut(s) 1344, 1798
Tru9I TTAA 2 cut(s) 1344, 1798
TscAI CASTG 5 cut(s) 394, 1170, 1331, 1614, 1737
TseFI GTSAC 1 cut(s) 1292
TseI GCWGC 1 cut(s) 669
Tsp45I GTSAC 1 cut(s) 1292
TspDTI ATGAA 2 cut(s) 4, 1077
TspGWI ACGGA 1 cut(s) 1638
TspRI CASTG 5 cut(s) 394, 1170, 1331, 1614, 1737
VpaK11BI GGWCC 3 cut(s) 1054, 1540, 1675
XbaI TCTAGA 1 cut(s) 1375
XceI RCATGY 2 cut(s) 995, 1082
XcmI CCANNNNNNNNNTGG 1 cut(s) 1745
XspI CTAG 3 cut(s) 117, 1376, 1760
Zsp2I ATGCAT 1 cut(s) 1080
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.