AT5G01630

Protein BREAST CANCER SUSCEPTIBILITY 2 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0004075
Physical Location & Seq
Reverse (-)
234851 .. 241106
6256 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G01630.1

Sequence Viewer

Length: 3468 bp
ATGTCGACGTGGCATTTATTTTCCGATTCCAGCGGCGATGGTTTCCGGTGGGAAGTCGCTGGCCGGATTCTTCAGTCCGTCTCGGATTCTACGCCAACCAAGGCGCTCGAATCGACAGCTCCTCTACCTTCCATGGCCGATCTCTTGCTCCAAGGATGCTCGAAGCTTATAGAACGGGAAGAATCGATGCCTGGGGAAATCCCAATGTTCAGGACTGGGCTGGGGAAGTCTGTAGTTCTTAAAGAGTCTTCGATTGCTAAAGCCAAATCCATTTTAGCAGAGAATGTTGCTTATTCAGATTTGCAGAACACAAATTGCTCAATTCCCCAAACGAGACAAGTGGATACAGCTGAAACTATGCCAATGTTCAGGACGGCCTTGGGGAAAACTGTCCCTTTGAAAGAGTCTTCAATTGCCAAACCTTTGTCTATTCTAGGAAGTGATATGATAATTGACTCAGACAACGTTCTTCCCAGGGAGAGTGGGTTTGGTGTTCCCAACAGTCTTTTTCAGACAGCTTCCAACAAAAAGGTTAATGTATCTTCTGCCGGTTTAGCAAGAGCCAAGGCATTGTTAGGACTAGAAGAAGATGATTTGAATGGATTTAACCATGTGAACCAGTCGTCTTCTTCCCTCCAGCAACATGGGTGGTCCGGTTTAAAAACTCATGAGGAGTTTGATGCTACAGTGGTTAAGCATCATTCAGGAACTCCAGGACAATATGAGAATTATGTATCTGGGAAGAGATCTGAAATCTTAAATCCATCGTTAAAGGTGCCTCCAACAAAGTTTCAGACTGCTGGTGGAAAATCATTGTCAGTGTCAGCTGAGGCGTTGAAACGTGCCAGAAACCTTCTTGGTGACCCTGAGTTAGGGTCTTTCTTTGATGATGTAGCTGGAGGTGATCAATTTTTTACACCACAGAAAGATGAAAGGCTAAGTGATATTGCTATAAACAATGGAAGTGTCAACACAGGTTATATTGCTCATGAAGAAAAGACAAGCAACAAACATACGTCAAATAGTTTTGTATCTCCTCTTCATTCATCTTCAAAGCAATTTAGATCAGTTAACTTGGAGAACCTAGCTTCAGGGGGTAATTTGATCAAGAAATTTGATACGGCTGTTGATGAGACCAATTGTGCTCTGAATATCAGTAAACCTGCTACACATGGACTATCAAATAACAGGCCTTTAGCATCAGATATGGCAGTGAATAATTCCAAGGGAAACGGTTTCATTCCAAGAGCTAGGCAACTTGGAAGGCCTGCTGATCAGCCACTTGTTGATATAACAAATCGCAGAGATACAGCTTATGCAAACAATAAACAAGACAGTACCCAAAAGAAAAGGCTGGGAAAGACAGTCTCCGTTTCTCCTTTCAAAAGGCCAAGGATTTCATCCTTTAAAACTCCTTTAAAGAAAAATGCTCAGCAAGCTTCAAGTGGTTTGTCTGTTGTATCGTGTGATACACTTACTTCCAAAAAGGTACTTTCTACAAGATATCCAGAAAAGTCTCCAAGAGTATATATCAAGGAGTTTTTTGGAATGCATCCGACAGCTACGACCAGGATGGATTATGTGCCAGACCATGTCAGAAGAATCAAATCAAGTAATGCAGATAAATATGTATTCTGTGATGAGTCTTCTTCAAACAAGGTTGGAGCTGAAACTTTTCTTCAAATGTTGGCTGAGTCGGGTGCTTCCTTACAACATGCATCCAGAAAGTGGGTTACTAATCACTACAGGTGGATAGTCTGGAAACTTGCATGCTACGACATATACTACCCAGCCAAATGCAGAGGAAACTTTCTAACAATCACCAATGTTCTTGAGGAACTAAAATACAGATATGAGAGAGAGGTCAATCATGGTCATTGCTCTGCAATCAAGAGGATTCTGAGTGGTGATGCTCCAGCTTCTTCAATGATGGTCCTCTGCATATCAGCTATTAATCCAAGGACCGATAATGGTTCTCAGGAAGCACATTGTTCTGATAATTGCAGCAATGTGAAAGTCGAACTCACTGATGGGTGGTACTCGATGAATGCTGCTCTGGATGTCGTGCTAACCAAGCAGCTGAATGCTGGCAAATTGTTTGTGGGGCAGAAGCTTCGAATCCTCGGGGCAGGACTTTCTGGCTGGGCTACCCCAACATCACCACTTGAGGCAGTGATTTCAAGTACAATCTGTTTGTTGTTGAATATTAATGGGACATACAGAGCTCACTGGGCGGACCGACTAGGCTTCTGTAAAGAAATTGGTGTCCCTCTGGCTTTCAACTGTATCAAGTGCAATGGGGGTCCAGTGCCTAAAACGCTAGCTGGCATCACACGAATATACCCCATCCTATACAAGGAAAGGTTAGGTGAAAAGAAGTCAATAGTTCGATCAGAGAGAATAGAAAGTAGAATTATACAGCTGCATAATCAGAGGCGCTCAGCTCTTGTTGAGGGTATTATGTGTGAGTACCAGAGAGGAATTAATGGTGTTCACAGCCAAAACGATACTGACAGCGAAGAAGGAGCCAAGGTCTTTAAGCTGTTAGAGACGGCTGCTGAACCTGAACTTCTTATGGCTGAAATGAGCCTAGAGCAGTTGACATCTTTCACTACATATAAAGCAAAATTTGAGGCAGCCAAGCAAATGCAGATGGAAAAATCAGTGGCAAAAGCTCTGGAAGACGCTGGTTTAGGTGAAAGGAACGTCACCCCATTCATGAGGATTAGGCTTGTTGGCCTGACGAGCTTAAGTAATGAAGGAGAGCACAATCCAAAAGAAGGCATTGTAACAATCTGGGATCCCACGGAGAGACAGAGAACCGAGCTGACGGAGGGGAAAATATACATAATGAAAGGGCTAGTACCAATGAACTCAGATTCAGAAACACTTTACTTACATGCCAGAGGGTCAAGCTCAAGATGGCAGCCTTTGTCTCCAAAAGATTCTGAAAATTTTCAGCCTTTTTTCAACCCTCGTAAACCAATCTCTTTGTCAAATCTTGGTGAAATTCCTCTGTCAAGTGAATTTGATATCGCTGCGTACGTTGTATATGTTGGAGATGCATACACAGATGTTCTACAAAAGAAGCAGTGGGTGTTTGTGACAGATGGATCCACACAACATTCAGGGGAAATCTCAAACAGTCTTCTTGCTATAAGCTTCAGCACCCCATTCATGGATGATAGCTCTGTTTCCCACATCAGCCATAATTTGGTTGGATCCGTGGTCGGATTTTGCAATCTAATTAAAAGAGCAAAGGATGCGACAAACGAAATGTGGGTTGCTGAGACAACAGAGAACTCAGTCTACTTCATAAACGCAGAAGCTGCCTACTCTTCTCACCTTAAAACCCGCAGTGCCCATATCCAAACATGGGCTAAGCTCTATTCTTCCAAATCCGTCATCCATGAACTTCGGCAAAGAGTCCTATTTATAATCGGTGCTTGTAAATCACCTTCATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000226 GO:0000228 GO:0000278 GO:0000280 GO:0000281 GO:0000722 GO:0000723 GO:0000724 GO:0000725 GO:0000781 GO:0000784 GO:0000793 GO:0000794 GO:0000795 GO:0000800 GO:0000910 GO:0001556 GO:0001701 GO:0001824 GO:0001832 GO:0001833 GO:0002020 GO:0002376 GO:0002520 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003697 GO:0003824 GO:0004402 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005739 GO:0005813 GO:0005815 GO:0005829 GO:0005856 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006275 GO:0006281 GO:0006289 GO:0006302 GO:0006310 GO:0006312 GO:0006325 GO:0006355 GO:0006464 GO:0006473 GO:0006475 GO:0006725 GO:0006807 GO:0006915 GO:0006950 GO:0006974 GO:0006978 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007059 GO:0007098 GO:0007127 GO:0007140 GO:0007141 GO:0007154 GO:0007165 GO:0007275 GO:0007276 GO:0007281 GO:0007283 GO:0007292 GO:0007346 GO:0007399 GO:0007417 GO:0007420 GO:0007548 GO:0007568 GO:0007569 GO:0008022 GO:0008080 GO:0008092 GO:0008104 GO:0008150 GO:0008152 GO:0008156 GO:0008219 GO:0008283 GO:0008285 GO:0008406 GO:0008585 GO:0008608 GO:0008630 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009790 GO:0009792 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0009994 GO:0010165 GO:0010212 GO:0010225 GO:0010332 GO:0010468 GO:0010484 GO:0010485 GO:0010556 GO:0010557 GO:0010558 GO:0010564 GO:0010604 GO:0010605 GO:0010628 GO:0012501 GO:0012505 GO:0015630 GO:0015631 GO:0016043 GO:0016407 GO:0016410 GO:0016569 GO:0016570 GO:0016573 GO:0016740 GO:0016746 GO:0016747 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019538 GO:0019899 GO:0019953 GO:0021700 GO:0022402 GO:0022412 GO:0022414 GO:0023052 GO:0030097 GO:0030141 GO:0030154 GO:0030330 GO:0030879 GO:0031023 GO:0031297 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031410 GO:0031619 GO:0031974 GO:0031981 GO:0031982 GO:0032200 GO:0032465 GO:0032501 GO:0032502 GO:0032504 GO:0032991 GO:0033036 GO:0033260 GO:0033365 GO:0033554 GO:0033593 GO:0033599 GO:0033600 GO:0034212 GO:0034502 GO:0034613 GO:0034641 GO:0034645 GO:0035264 GO:0035556 GO:0036211 GO:0040007 GO:0042127 GO:0042592 GO:0042770 GO:0042771 GO:0042772 GO:0042802 GO:0043009 GO:0043015 GO:0043060 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043543 GO:0043966 GO:0043967 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044454 GO:0044464 GO:0044703 GO:0044786 GO:0045005 GO:0045132 GO:0045137 GO:0045143 GO:0045184 GO:0045787 GO:0045893 GO:0045931 GO:0045934 GO:0045935 GO:0046483 GO:0046545 GO:0046660 GO:0048232 GO:0048285 GO:0048468 GO:0048469 GO:0048477 GO:0048478 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048534 GO:0048589 GO:0048599 GO:0048608 GO:0048609 GO:0048731 GO:0048732 GO:0048856 GO:0048869 GO:0050000 GO:0050678 GO:0050680 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051052 GO:0051053 GO:0051093 GO:0051171 GO:0051172 GO:0051173 GO:0051179 GO:0051234 GO:0051239 GO:0051241 GO:0051252 GO:0051254 GO:0051276 GO:0051298 GO:0051301 GO:0051302 GO:0051303 GO:0051310 GO:0051311 GO:0051316 GO:0051321 GO:0051455 GO:0051640 GO:0051641 GO:0051649 GO:0051656 GO:0051704 GO:0051716 GO:0051726 GO:0060249 GO:0060255 GO:0060322 GO:0061458 GO:0061640 GO:0061733 GO:0061982 GO:0065007 GO:0065008 GO:0070013 GO:0070198 GO:0070199 GO:0070200 GO:0070727 GO:0071695 GO:0071704 GO:0071840 GO:0072331 GO:0072332 GO:0072594 GO:0080090 GO:0090304 GO:0090329 GO:0097159 GO:0097190 GO:0097193 GO:0097708 GO:0098687 GO:0098813 GO:0099086 GO:0099503 GO:0140013 GO:1901360 GO:1901363 GO:1901564 GO:1901576 GO:1902298 GO:1902680 GO:1902969 GO:1903046 GO:1903047 GO:1903506 GO:1903508 GO:1990426 GO:1990505 GO:2000026 GO:2000104 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03440 ko03460 ko05200 ko05212 ko05224 map03440 map03460 map05200 map05212 map05224
Pfam Domains
Protein Families
BRCA-2_OB1 BRCA-2_helical BRCA2

Protein Analysis

1155

Amino Acids

127.25

Weight (kDa)

8.77

Isoelectric Point (pI)

40.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BRCA2 PF00634 167 - 193 5.4e-08 BRCA2 repeat
BRCA2 PF00634 261 - 288 8.7e-09 BRCA2 repeat
BRCA-2_helical PF09169 550 - 624 7e-16 BRCA2, helical
BRCA-2_OB1 PF09103 628 - 752 5.6e-38 BRCA2, oligonucleotide/oligosaccharide-binding, domain 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3437
Acc36I ACCTGC 1 cut(s) 1171
AccB1I GGYRCC 1 cut(s) 775
AccB7I CCANNNNNTGG 2 cut(s) 1728, 3214
AccI GTMKAC 2 cut(s) 5, 3309
AciI CCGC 3 cut(s) 33, 2234, 3355
AclI AACGTT 1 cut(s) 465
AclWI GGATC 6 cut(s) 2795, 2808, 3108, 3121, 3216, 3229
AcoI YGGCCR 2 cut(s) 61, 135
AcsI RAATTY 5 cut(s) 1112, 2627, 2953, 3009, 3026
AcuI CTGAAG 3 cut(s) 56, 1074, 3148
AfaI GTAC 7 cut(s) 1339, 1491, 2039, 2185, 2471, 2865, 3044
AfiI CCNNNNNNNGG 7 cut(s) 872, 1728, 2356, 2780, 3178, 3214, 3376
AflII CTTAAG 1 cut(s) 2749
AjiI CACGTC 1 cut(s) 9
AjnI CCWGG 4 cut(s) 190, 473, 712, 1568
Alw21I GWGCWC 3 cut(s) 1147, 2227, 2769
Alw26I GTCTC 9 cut(s) 85, 328, 1127, 1372, 1521, 2543, 2806, 2940, 3284
AlwI GGATC 6 cut(s) 2795, 2808, 3108, 3121, 3216, 3229
AlwNI CAGNNNCTG 1 cut(s) 3329
Ama87I CYCGRG 1 cut(s) 2123
AoxI GGCC 7 cut(s) 61, 135, 375, 1190, 1265, 1388, 2737
ApeKI GCWGC 9 cut(s) 2004, 2051, 2077, 2422, 2555, 2636, 2926, 3038, 3329
ApoI RAATTY 5 cut(s) 1112, 2627, 2953, 3009, 3026
AseI ATTAAT 3 cut(s) 1953, 2208, 2484
Asp700I GAANNNNTTC 2 cut(s) 1674, 2955
AspLEI GCGC 2 cut(s) 106, 2439
AspS9I GGNCC 5 cut(s) 651, 1933, 1962, 2236, 2303
AsuII TTCGAA 1 cut(s) 2116
AsuNHI GCTAGC 1 cut(s) 2320
AvaI CYCGRG 1 cut(s) 2123
AvaII GGWCC 5 cut(s) 651, 1933, 1962, 2236, 2303
BaeGI GKGCMC 1 cut(s) 3364
BaeI ACNNNNGTAYC 2 cut(s) 1110, 1143
BamHI GGATCC 3 cut(s) 2800, 3113, 3221
BanI GGYRCC 1 cut(s) 775
BanII GRGCYC 1 cut(s) 2227
BarI GAAGNNNNNNTAC 3 cut(s) 1023, 1055, 3442
BbsI GAAGAC 6 cut(s) 240, 399, 618, 1638, 2688, 3140
Bbv12I GWGCWC 3 cut(s) 1147, 2227, 2769
BbvCI CCTCAGC 1 cut(s) 828
BbvI GCAGC 9 cut(s) 2016, 2038, 2089, 2409, 2542, 2648, 2938, 3025, 3316
BccI CCATC 9 cut(s) 32, 772, 1567, 1924, 2024, 2354, 2647, 2916, 3104
BceAI ACGGC 3 cut(s) 390, 1137, 2568
BcgI CGANNNNNNTGC 2 cut(s) 2096, 2130
BciT130I CCWGG 4 cut(s) 192, 475, 714, 1570
BciVI GTATCC 1 cut(s) 337
BclI TGATCA 3 cut(s) 904, 1104, 1273
BcoDI GTCTC 9 cut(s) 85, 328, 1127, 1372, 1521, 2543, 2806, 2940, 3284
BfaI CTAG 8 cut(s) 434, 581, 1085, 1251, 2243, 2321, 2591, 2861
BfmI CTRYAG 3 cut(s) 231, 684, 1744
BfoI RGCGCY 2 cut(s) 107, 2440
BfrI CTTAAG 1 cut(s) 2749
BfuAI ACCTGC 1 cut(s) 1171
BfuI GTATCC 1 cut(s) 337
BglII AGATCT 1 cut(s) 746
BlpI GCTNAGC 3 cut(s) 1431, 2440, 3381
Bme1390I CCNGG 4 cut(s) 192, 475, 714, 1570
Bme18I GGWCC 5 cut(s) 651, 1933, 1962, 2236, 2303
BmeT110I CYCGRG 1 cut(s) 2123
BmgBI CACGTC 1 cut(s) 9
BmgT120I GGNCC 5 cut(s) 651, 1933, 1962, 2236, 2303
BmiI GGNNCC 6 cut(s) 777, 2304, 2527, 2802, 3115, 3223
BmrFI CCNGG 4 cut(s) 192, 475, 714, 1570
BmrI ACTGGG 2 cut(s) 225, 2239
BmtI GCTAGC 1 cut(s) 2324
BmuI ACTGGG 2 cut(s) 225, 2239
BpiI GAAGAC 6 cut(s) 240, 399, 618, 1638, 2688, 3140
BpmI CTGGAG 4 cut(s) 620, 696, 918, 1899
Bpu10I CCTNAGC 1 cut(s) 828
Bpu1102I GCTNAGC 3 cut(s) 1431, 2440, 3381
Bpu14I TTCGAA 1 cut(s) 2116
BpuEI CTTGAG 3 cut(s) 1853, 2186, 2902
Bsa29I ATCGAT 1 cut(s) 185
BsaI GGTCTC 1 cut(s) 1127
BsaWI WCCGGW 2 cut(s) 45, 653
BsaXI ACNNNNNCTCC 4 cut(s) 470, 500, 2517, 2547
Bsc4I CCNNNNNNNGG 7 cut(s) 872, 1728, 2356, 2780, 3178, 3214, 3376
Bse118I RCCGGY 1 cut(s) 548
Bse1I ACTGG 4 cut(s) 220, 619, 2234, 2306
Bse3DI GCAATG 3 cut(s) 1876, 2014, 2302
BseBI CCWGG 4 cut(s) 192, 475, 714, 1570
BseCI ATCGAT 1 cut(s) 185
BseLI CCNNNNNNNGG 7 cut(s) 872, 1728, 2356, 2780, 3178, 3214, 3376
BseMI GCAATG 3 cut(s) 1876, 2014, 2302
BseNI ACTGG 4 cut(s) 220, 619, 2234, 2306
BseRI GAGGAG 3 cut(s) 111, 686, 1026
BseSI GKGCMC 1 cut(s) 3364
BseXI GCAGC 9 cut(s) 2016, 2038, 2089, 2409, 2542, 2648, 2938, 3025, 3316
BseYI CCCAGC 4 cut(s) 220, 1354, 1789, 2142
BshFI GGCC 7 cut(s) 63, 137, 377, 1192, 1267, 1390, 2739
BshNI GGYRCC 1 cut(s) 775
BshVI ATCGAT 1 cut(s) 185
BsiHKAI GWGCWC 3 cut(s) 1147, 2227, 2769
BsiHKCI CYCGRG 1 cut(s) 2123
BsiSI CCGG 4 cut(s) 46, 64, 549, 654
BsiWI CGTACG 1 cut(s) 3042
BslFI GGGAC 3 cut(s) 377, 2227, 2252
BslI CCNNNNNNNGG 7 cut(s) 872, 1728, 2356, 2780, 3178, 3214, 3376
BsmAI GTCTC 9 cut(s) 85, 328, 1127, 1372, 1521, 2543, 2806, 2940, 3284
BsmBI CGTCTC 2 cut(s) 85, 2543
BsmFI GGGAC 3 cut(s) 377, 2227, 2252
BsmI GAATGC 3 cut(s) 1554, 2053, 2089
BsnI GGCC 7 cut(s) 63, 137, 377, 1192, 1267, 1390, 2739
Bso31I GGTCTC 1 cut(s) 1127
BsoBI CYCGRG 1 cut(s) 2123
Bsp119I TTCGAA 1 cut(s) 2116
Bsp1286I GDGCHC 4 cut(s) 1147, 2227, 2769, 3364
Bsp1720I GCTNAGC 3 cut(s) 1431, 2440, 3381
Bsp19I CCATGG 1 cut(s) 132
BspACI CCGC 3 cut(s) 33, 2234, 3355
BspANI GGCC 7 cut(s) 63, 137, 377, 1192, 1267, 1390, 2739
BspDI ATCGAT 1 cut(s) 185
BspHI TCATGA 3 cut(s) 667, 988, 2718
BspLI GGNNCC 6 cut(s) 777, 2304, 2527, 2802, 3115, 3223
BspMI ACCTGC 1 cut(s) 1171
BspOI GCTAGC 1 cut(s) 2324
BspPI GGATC 6 cut(s) 2795, 2808, 3108, 3121, 3216, 3229
BspT104I TTCGAA 1 cut(s) 2116
BspT107I GGYRCC 1 cut(s) 775
BspTI CTTAAG 1 cut(s) 2749
BspTNI GGTCTC 1 cut(s) 1127
BsrDI GCAATG 3 cut(s) 1876, 2014, 2302
BsrFI RCCGGY 1 cut(s) 548
BsrI ACTGG 4 cut(s) 220, 619, 2234, 2306
BssAI RCCGGY 1 cut(s) 548
BssT1I CCWWGG 9 cut(s) 99, 132, 151, 378, 564, 1224, 1391, 1958, 2529
Bst2UI CCWGG 4 cut(s) 192, 475, 714, 1570
Bst4CI ACNGT 8 cut(s) 391, 503, 688, 1235, 1337, 1366, 2285, 3146
Bst6I CTCTTC 3 cut(s) 737, 1044, 3343
BstAFI CTTAAG 1 cut(s) 2749
BstAPI GCANNNNNTGC 2 cut(s) 3263, 3329
BstBI TTCGAA 1 cut(s) 2116
BstC8I GCNNGC 7 cut(s) 61, 1269, 1437, 1771, 2089, 2322, 2326
BstDSI CCRYGG 3 cut(s) 132, 2805, 3225
BstEII GGTNACC 1 cut(s) 860
BstENI CCTNNNNNAGG 2 cut(s) 870, 2354
BstH2I RGCGCY 2 cut(s) 107, 2440
BstHHI GCGC 2 cut(s) 106, 2439
BstMAI GTCTC 9 cut(s) 85, 328, 1127, 1372, 1521, 2543, 2806, 2940, 3284
BstMWI GCNNNNNNNGC 8 cut(s) 554, 1436, 2074, 2231, 2317, 2745, 3263, 3329
BstNI CCWGG 4 cut(s) 192, 475, 714, 1570
BstNSI RCATGY 3 cut(s) 1718, 1773, 2903
BstPI GGTNACC 1 cut(s) 860
BstSCI CCNGG 4 cut(s) 190, 473, 712, 1568
BstSFI CTRYAG 3 cut(s) 231, 684, 1744
BstSLI GKGCMC 1 cut(s) 3364
BstV1I GCAGC 9 cut(s) 2016, 2038, 2089, 2409, 2542, 2648, 2938, 3025, 3316
BstV2I GAAGAC 6 cut(s) 240, 399, 618, 1638, 2688, 3140
BstX2I RGATCY 4 cut(s) 746, 2800, 3113, 3221
BstXI CCANNNNNNTGG 1 cut(s) 644
BstYI RGATCY 4 cut(s) 746, 2800, 3113, 3221
Bsu15I ATCGAT 1 cut(s) 185
BsuI GTATCC 1 cut(s) 337
BsuRI GGCC 7 cut(s) 63, 137, 377, 1192, 1267, 1390, 2739
BsuTUI ATCGAT 1 cut(s) 185
BtgI CCRYGG 3 cut(s) 132, 2805, 3225
BtgZI GCGATG 1 cut(s) 51
BtrI CACGTC 1 cut(s) 9
BtsI GCAGTG 4 cut(s) 1218, 2178, 3098, 3364
BveI ACCTGC 1 cut(s) 1171
Cac8I GCNNGC 7 cut(s) 61, 1269, 1437, 1771, 2089, 2322, 2326
CaiI CAGNNNCTG 1 cut(s) 3329
CciI TCATGA 3 cut(s) 667, 988, 2718
CfoI GCGC 2 cut(s) 106, 2439
Cfr10I RCCGGY 1 cut(s) 548
Cfr13I GGNCC 5 cut(s) 651, 1933, 1962, 2236, 2303
ClaI ATCGAT 1 cut(s) 185
CpoI CGGWCCG 1 cut(s) 2236
CseI GACGC 1 cut(s) 2693
Csp6I GTAC 7 cut(s) 1338, 1490, 2038, 2184, 2470, 2864, 3043
CspCI CAANNNNNGTGG 2 cut(s) 629, 664
CspI CGGWCCG 1 cut(s) 2236
CviQI GTAC 7 cut(s) 1338, 1490, 2038, 2184, 2470, 2864, 3043
DraI TTTAAA 3 cut(s) 660, 1408, 1419
EaeI YGGCCR 2 cut(s) 61, 135
Eam1104I CTCTTC 3 cut(s) 737, 1044, 3343
EarI CTCTTC 3 cut(s) 737, 1044, 3343
EciI GGCGGA 1 cut(s) 2249
Ecl136II GAGCTC 1 cut(s) 2225
Eco130I CCWWGG 9 cut(s) 99, 132, 151, 378, 564, 1224, 1391, 1958, 2529
Eco147I AGGCCT 2 cut(s) 1192, 1267
Eco24I GRGCYC 1 cut(s) 2227
Eco31I GGTCTC 1 cut(s) 1127
Eco32I GATATC 2 cut(s) 1505, 3034
Eco47I GGWCC 5 cut(s) 651, 1933, 1962, 2236, 2303
Eco53kI GAGCTC 1 cut(s) 2225
Eco57I CTGAAG 3 cut(s) 56, 1074, 3148
Eco88I CYCGRG 1 cut(s) 2123
Eco91I GGTNACC 1 cut(s) 860
EcoICRI GAGCTC 1 cut(s) 2225
EcoNI CCTNNNNNAGG 2 cut(s) 870, 2354
EcoO65I GGTNACC 1 cut(s) 860
EcoRII CCWGG 4 cut(s) 190, 473, 712, 1568
EcoRV GATATC 2 cut(s) 1505, 3034
EcoT14I CCWWGG 9 cut(s) 99, 132, 151, 378, 564, 1224, 1391, 1958, 2529
EcoT22I ATGCAT 3 cut(s) 1554, 1720, 3067
EcoT38I GRGCYC 1 cut(s) 2227
ErhI CCWWGG 9 cut(s) 99, 132, 151, 378, 564, 1224, 1391, 1958, 2529
Esp3I CGTCTC 2 cut(s) 85, 2543
FaqI GGGAC 3 cut(s) 377, 2227, 2252
FauI CCCGC 1 cut(s) 3362
FbaI TGATCA 3 cut(s) 904, 1104, 1273
FblI GTMKAC 2 cut(s) 5, 3309
FriOI GRGCYC 1 cut(s) 2227
FspBI CTAG 8 cut(s) 434, 581, 1085, 1251, 2243, 2321, 2591, 2861
GlaI GCGC 2 cut(s) 105, 2438
GsaI CCCAGC 4 cut(s) 224, 1358, 1793, 2146
GsuI CTGGAG 4 cut(s) 620, 696, 918, 1899
HaeII RGCGCY 2 cut(s) 107, 2440
HaeIII GGCC 7 cut(s) 63, 137, 377, 1192, 1267, 1390, 2739
HapII CCGG 4 cut(s) 46, 64, 549, 654
HgaI GACGC 1 cut(s) 2693
HhaI GCGC 2 cut(s) 106, 2439
Hin6I GCGC 2 cut(s) 104, 2437
HinP1I GCGC 2 cut(s) 104, 2437
HincII GTYRAC 4 cut(s) 6, 970, 1072, 2601
HindII GTYRAC 4 cut(s) 6, 970, 1072, 2601
HindIII AAGCTT 4 cut(s) 164, 1437, 2111, 3160
HpaI GTTAAC 1 cut(s) 1072
HpaII CCGG 4 cut(s) 46, 64, 549, 654
Hpy166II GTNNAC 9 cut(s) 6, 616, 970, 1072, 1160, 2494, 2601, 2981, 3310
Hpy8I GTNNAC 9 cut(s) 6, 616, 970, 1072, 1160, 2494, 2601, 2981, 3310
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 7 cut(s) 138, 863, 1257, 2516, 2753, 2774, 3468
HpyCH4III ACNGT 8 cut(s) 391, 503, 688, 1235, 1337, 1366, 2285, 3146
HpyCH4IV ACGT 6 cut(s) 8, 465, 841, 1016, 2706, 3045
HpyF10VI GCNNNNNNNGC 8 cut(s) 554, 1436, 2074, 2231, 2317, 2745, 3263, 3329
HpySE526I ACGT 6 cut(s) 8, 465, 841, 1016, 2706, 3045
HspAI GCGC 2 cut(s) 104, 2437
Ksp22I TGATCA 3 cut(s) 904, 1104, 1273
KspAI GTTAAC 1 cut(s) 1072
LmnI GCTCC 5 cut(s) 124, 153, 1664, 1918, 2525
Lsp1109I GCAGC 9 cut(s) 2016, 2038, 2089, 2409, 2542, 2648, 2938, 3025, 3316
MaeI CTAG 8 cut(s) 434, 581, 1085, 1251, 2243, 2321, 2591, 2861
MaeII ACGT 6 cut(s) 8, 465, 841, 1016, 2706, 3045
MaeIII GTNAC 5 cut(s) 860, 1732, 2707, 2788, 3103
MfeI CAATTG 2 cut(s) 411, 1138
MflI RGATCY 4 cut(s) 746, 2800, 3113, 3221
MhlI GDGCHC 4 cut(s) 1147, 2227, 2769, 3364
MlyI GAGTC 6 cut(s) 254, 413, 449, 1652, 1703, 3435
MmeI TCCRAC 7 cut(s) 546, 806, 1580, 1642, 3037, 3199, 3211
Mph1103I ATGCAT 3 cut(s) 1554, 1720, 3067
MroXI GAANNNNTTC 2 cut(s) 1674, 2955
MslI CAYNNNNRTG 1 cut(s) 3460
MspA1I CMGCKG 5 cut(s) 33, 350, 827, 2080, 2422
MspCI CTTAAG 1 cut(s) 2749
MspI CCGG 4 cut(s) 46, 64, 549, 654
MspR9I CCNGG 4 cut(s) 192, 475, 714, 1570
MunI CAATTG 2 cut(s) 411, 1138
Mva1269I GAATGC 3 cut(s) 1554, 2053, 2089
MvaI CCWGG 4 cut(s) 192, 475, 714, 1570
MwoI GCNNNNNNNGC 8 cut(s) 554, 1436, 2074, 2231, 2317, 2745, 3263, 3329
NcoI CCATGG 1 cut(s) 132
NheI GCTAGC 1 cut(s) 2320
NlaIV GGNNCC 6 cut(s) 777, 2304, 2527, 2802, 3115, 3223
NmuCI GTSAC 3 cut(s) 860, 2707, 3103
NsiI ATGCAT 3 cut(s) 1554, 1720, 3067
NspI RCATGY 3 cut(s) 1718, 1773, 2903
NspV TTCGAA 1 cut(s) 2116
PaeI GCATGC 1 cut(s) 1773
PagI TCATGA 3 cut(s) 667, 988, 2718
PasI CCCWGGG 1 cut(s) 474
PceI AGGCCT 2 cut(s) 1192, 1267
PcsI WCGNNNNNNNCGW 1 cut(s) 3042
PctI GAATGC 3 cut(s) 1554, 2053, 2089
PdmI GAANNNNTTC 2 cut(s) 1674, 2955
Pfl23II CGTACG 1 cut(s) 3042
PflFI GACNNNGTC 1 cut(s) 1592
PflMI CCANNNNNTGG 2 cut(s) 1728, 3214
PfoI TCCNGGA 1 cut(s) 712
PleI GAGTC 6 cut(s) 253, 412, 449, 1651, 1702, 3434
PpsI GAGTC 6 cut(s) 253, 412, 449, 1651, 1702, 3434
PshBI ATTAAT 3 cut(s) 1953, 2208, 2484
PsiI TTATAA 1 cut(s) 3437
Psp124BI GAGCTC 1 cut(s) 2227
Psp1406I AACGTT 1 cut(s) 465
Psp6I CCWGG 4 cut(s) 190, 473, 712, 1568
PspEI GGTNACC 1 cut(s) 860
PspFI CCCAGC 4 cut(s) 220, 1354, 1789, 2142
PspGI CCWGG 4 cut(s) 190, 473, 712, 1568
PspLI CGTACG 1 cut(s) 3042
PspN4I GGNNCC 6 cut(s) 777, 2304, 2527, 2802, 3115, 3223
PspPI GGNCC 5 cut(s) 651, 1933, 1962, 2236, 2303
PsrI GAACNNNNNNTAC 2 cut(s) 3293, 3325
PstNI CAGNNNCTG 1 cut(s) 3329
PsuI RGATCY 4 cut(s) 746, 2800, 3113, 3221
PsyI GACNNNGTC 1 cut(s) 1592
PvuII CAGCTG 4 cut(s) 350, 827, 2080, 2422
RsaI GTAC 7 cut(s) 1339, 1491, 2039, 2185, 2471, 2865, 3044
RsaNI GTAC 7 cut(s) 1338, 1490, 2038, 2184, 2470, 2864, 3043
RseI CAYNNNNRTG 1 cut(s) 3460
Rsr2I CGGWCCG 1 cut(s) 2236
RsrII CGGWCCG 1 cut(s) 2236
SacI GAGCTC 1 cut(s) 2227
SalI GTCGAC 1 cut(s) 4
Sau96I GGNCC 5 cut(s) 651, 1933, 1962, 2236, 2303
SchI GAGTC 6 cut(s) 254, 413, 449, 1652, 1703, 3435
ScrFI CCNGG 4 cut(s) 192, 475, 714, 1570
SduI GDGCHC 4 cut(s) 1147, 2227, 2769, 3364
SfcI CTRYAG 3 cut(s) 231, 684, 1744
SfuI TTCGAA 1 cut(s) 2116
SinI GGWCC 5 cut(s) 651, 1933, 1962, 2236, 2303
SmiMI CAYNNNNRTG 1 cut(s) 3460
SmlI CTYRAG 4 cut(s) 1832, 2165, 2749, 2917
SmoI CTYRAG 4 cut(s) 1832, 2165, 2749, 2917
SphI GCATGC 1 cut(s) 1773
SseBI AGGCCT 2 cut(s) 1192, 1267
SsiI CCGC 3 cut(s) 33, 2234, 3355
SspI AATATT 1 cut(s) 2206
SspMI CTAG 8 cut(s) 434, 581, 1085, 1251, 2243, 2321, 2591, 2861
SstI GAGCTC 1 cut(s) 2227
StuI AGGCCT 2 cut(s) 1192, 1267
StyD4I CCNGG 4 cut(s) 190, 473, 712, 1568
StyI CCWWGG 9 cut(s) 99, 132, 151, 378, 564, 1224, 1391, 1958, 2529
TaaI ACNGT 8 cut(s) 391, 503, 688, 1235, 1337, 1366, 2285, 3146
TaiI ACGT 6 cut(s) 11, 468, 844, 1019, 2709, 3048
TaqII GACCGA 2 cut(s) 1979, 2253
TatI WGTACW 1 cut(s) 2183
TauI GCSGC 1 cut(s) 36
TseFI GTSAC 3 cut(s) 860, 2707, 3103
TseI GCWGC 9 cut(s) 2004, 2051, 2077, 2422, 2555, 2636, 2926, 3038, 3329
Tsp45I GTSAC 3 cut(s) 860, 2707, 3103
TspGWI ACGGA 6 cut(s) 67, 1360, 2822, 2846, 3214, 3391
Tth111I GACNNNGTC 1 cut(s) 1592
Van91I CCANNNNNTGG 2 cut(s) 1728, 3214
Vha464I CTTAAG 1 cut(s) 2749
VpaK11BI GGWCC 5 cut(s) 651, 1933, 1962, 2236, 2303
VspI ATTAAT 3 cut(s) 1953, 2208, 2484
XagI CCTNNNNNAGG 2 cut(s) 870, 2354
XapI RAATTY 5 cut(s) 1112, 2627, 2953, 3009, 3026
XceI RCATGY 3 cut(s) 1718, 1773, 2903
XcmI CCANNNNNNNNNTGG 1 cut(s) 3215
XmiI GTMKAC 2 cut(s) 5, 3309
XmnI GAANNNNTTC 2 cut(s) 1674, 2955
XspI CTAG 8 cut(s) 434, 581, 1085, 1251, 2243, 2321, 2591, 2861
Zsp2I ATGCAT 3 cut(s) 1554, 1720, 3067
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.