MD01G1008700.v1.1

BRCA2, oligonucleotide/oligosaccharide-binding, domain 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0004075
Physical Location & Seq
Reverse (-)
5558072 .. 5565764
7693 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1008700.v1.1.491

Sequence Viewer

Length: 3630 bp
ATGGCGACATGGCACATGTGCTCGGATGCCGGCAACAATTACCGGTGGTACCCGGACCCCAAACCCGAAGTCATAAACGGTGTCGTTTATCGTCCTAGTGAGAGTGATAGGAGCAGCAGCAGCCGGGTGCCGTCCATGGGGGATGTGTTGCTGCAAGGATGTTCTAAGCTGGCGGAGGCTGAGATTCGAAATCAGGTTGTTGAGGGAGGTGGCGTTGGGATGTTCCGCAACGGATTCGGAAAGCCCGTCGCCGTCAAACAATCTTCATTAGCCAAAGCATCTTCGTTGCTTCTTGGCTCCCAAATCGCAACACCCTCAGGCCAAGTGCAAGCTACGATTATGAATAACAGAAGTGGATGTGGAGGCGGAGGTGCAGGTGGAGGCGGAGGATGTGTAGGTGGTTTATCTAATTCCCTTTTCCACACTGGATCCGGTAAAATGGTTAATATATCCCCCGACAGTCTTGTCAGGGCGAAGACATTGCTTGGATTCCAAGACGATGACAATGCTCCCACCAAGATTGTGGGTTTTGGTTTTAACAGTGCTGCCCTGGTTGCAGGGACTCCTTTAATTATGAATAAGACTGTTTCACTGCAAACTAGATTTCAGAATGATCCTGAAAGGCTTAATTTAACTCCTGATAAAGTTAAACCTTCATCAATCAAGTTCCATACAGCAGGAGGTAGATCTATATCTGTTTCTACTGATGCAATACAACGTGCAAGGAGTCTACTTGGTGACCCAGAGTTGGGAAATTTCTTCAATGAGGCCAATGCAGGTGACTTGGGTTTCGCATTTTCTAAAGGTAGTCCACATGATAACCGCACTCCCATTTCCCACCAGAAGAAGATTAACAACTTTTTAACCAATACTTCTCCATCTCCACTGCAACCATTGCCTAATCAATTGCGGTCGTCTGCTATAAGTTCGGGTACTAACCTGATTACTCAATTCGATGGTGTTAGCAATGAAACTGCTTCTAGGTCAAACAGTAAGTTAGCTTATCGGCAGAATCCCCTTAGCAAGACCCACAATACAGTAGAAAACAATTGTTGGGCAAATGGTATTGATTTGAGGACTAATCATGTTGGAATGCCACCAGCCACGCCATTGTTTGATATTTCAAATGCTGTTGGCACACCAGCTACGAATAATACACAGATGACTGGTTTAAAGAGGAGGTTGGGAAGAAGTTCTATTTCTCCATTCAAGGCTTCTCCATCTCCACTGCAACCATTGTCTAATCAATTGTGGTCGTCAACTATAAGTTCGGGTACTAACCTCATTACCCAATTCGATGGTGTTAGCAATGAAACTGCTTCTAGGTCAAACAGTGAGTTACCTTATCGGCAGAATCCCCTTAGCAGGACCCACAATACAGTAGAAAACAATTGTTTGGCAAATGGTATTGGTTTGAGGACTAATCATGCTGGAAAGCCGCCAGCCAAGCCATTGGTTGATATTTCAAATACTGTTGGCACCACTGCTATGAATACTAAACAGATCACTGGTGTAAAGAGGAGGTTGGGAAGAAGTTCTATTTCTCCATTCAAGAAGCCTCGCACAAACTCCTCCACTCCATTGCCTAGGAATGTTCCCCTTGTTCCTAATGGTGTGTCTACATTGTCCTCTGATCATTCATGTTCCAAAAGAAGGGTTTCTACACGATATCCTTCACCCGTGACTAGAATGTATGTGAAGAAATACTTTGGAATGCCGCGACCTGACCAGAACATGTTGGAGTGTTTATCAGATCCAGTGAGAAGAATCACCGCAACTAATGCGGAAAAGTATACGTTTCTTGATGAGTCTGGCTTAAACTGCATAGGAGCAGAAGCTTTTGTCCACATGCTGGCCTGTTATGGAGCTTCAATGCAATATACCTCCAGAGAGTGGGTCACAAATCATTACAAGTGGGTTGTCTGGAAACTGGCTTGCTATGAGAGATATTATCCAGCTAAATCGTTTGGAAAATTCTTGACAGTCTCCAATGTACTCGAGGAATTGAAGTATAGATATGAAAGAGAAGTGAATCATGGTCACCGGTCTGCAATTAAGAAGATTCTGGAAGGAGATGCATCACCTTCTTCACCGGTGGTTCTATGCATTTCAGCTATTTGCTCAAATTGTGACCCAAATATGGAGACTAGCTCTGGAGCTGAAAATAGTAGTGCTGCGAAAGTCGAGCTTACTGATGGTTGGTATTCAGTGGATGCTGTTCTCGATGCACTCCTGTCAAAGCAGCTTGCTAGCGGAAAATTGTTTGTTGGACAGAAGCTTCGGATCTGGGGAGCAGCACTCAGTGGCTGGGCTGGGCCTGTTTCACCCCTCGAGGTTTCAAGACAAGTCTCTTTACGGCTGCACATAAACGGAACATATAGAGCTCATTGGGCTGATCAATTGGGATTTTGTAAAGGTGTTTGTGCTCCCTTAGCATTTAAGTGTGCCAAGAGTGGTGGAGGTCCAGTCCCTTGCACTCTTGTAGGAGTCAAACGGATATACCCTGTTCTTTACAAGGAGAGGTTAAGTAATGGAAGATCTATTGTAAGATCTGAGAGGTTGGAGACCCAAATGGTGGAATCACACCAACAGAGGCGCTCTAATGTTATAGAAGGCGTTATCTCTGAGTTCCAAAGAGGATTAGAACATTCGCATTTGTGTAATGATAGCGATGATGAAGGAGCAAAACTCTTGAAGATACTACAGACAGCAGCTGAACCTGAAATTTTAATGGCAGAAATGAGTGCAGAACAGTTGAAATCTTTTACCAAATATCAAGCAAAGCTAGAGGCCATCAAACAGTCGGACATGGAGAAATCAATTCTGAAAGCACTGGAAGATGCCGGCTTAAGTGAGAGGGAAGTCACGCCATTCATGAGAGTGAGGGTGGTTGGACTAACAAGAAAATTATACCAAGGGAAAGACTGTTCCAAAGAAGGCTTAATAACAATCTGGAACCCATCTGAGAAACAGAACTCTGAGCTGGTCGAGGGATGTGCATATAGCATTTCTGGACTTATACCAACAAGTTCTGATGGAGATATTTTAAAATTGCTGGCAAGAGGATCAACAACAAAATGGCAGCCTTTATCTCAGCAGGCAATAAACTACTTCGAGGGTCAGATCCACCTCAGGCCATTTTTCAGTCCTCGCGAATCAGTCTTATTGTCAGATTTGGGTAAAGTTCCTCTCTCCAGCGAATTTGATATTGCTGCATCCGTTGTGTTTGTGGGAGAGGCTTATATAGCTGCTCAGCAGAAGAAACAGTGGGTGTTTGTAACAGACGGCTCCAAATCTGAATTAAAGTCGGAAGGATTATGTGATTCTCTACTTGCAATCTGCTTCTCTTCACCATACATTGACAATGTTTCAAATGCTCCTATAAACTATAACCTCACAGGATCTACGGTTGGGTTTTGTAATCTGACCAAGAGAGAAAAGGATGAAATGAATTGCCTTTGGGTAGCTGAAGCTACAGAAAATTCAGCTTACTTTTTAACTTTTGACAATCCACGTTGTTCTCATCTCAAAGATGCTGCTGCCTCTAGTGAAAGATGGAAAAGGATGTCTATCCTGACTATCAATAGGCTAAAGGAGAGGGTTCTATTTATTATTGGGGCAAGTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000226 GO:0000228 GO:0000278 GO:0000280 GO:0000281 GO:0000722 GO:0000723 GO:0000724 GO:0000725 GO:0000781 GO:0000784 GO:0000793 GO:0000794 GO:0000795 GO:0000800 GO:0000910 GO:0001556 GO:0001701 GO:0001824 GO:0001832 GO:0001833 GO:0002020 GO:0002376 GO:0002520 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003697 GO:0003824 GO:0004402 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005739 GO:0005813 GO:0005815 GO:0005829 GO:0005856 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006275 GO:0006281 GO:0006289 GO:0006302 GO:0006310 GO:0006312 GO:0006325 GO:0006355 GO:0006464 GO:0006473 GO:0006475 GO:0006725 GO:0006807 GO:0006915 GO:0006950 GO:0006974 GO:0006978 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007059 GO:0007098 GO:0007127 GO:0007140 GO:0007141 GO:0007154 GO:0007165 GO:0007275 GO:0007276 GO:0007281 GO:0007283 GO:0007292 GO:0007346 GO:0007399 GO:0007417 GO:0007420 GO:0007548 GO:0007568 GO:0007569 GO:0008022 GO:0008080 GO:0008092 GO:0008104 GO:0008150 GO:0008152 GO:0008156 GO:0008219 GO:0008283 GO:0008285 GO:0008406 GO:0008585 GO:0008608 GO:0008630 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009790 GO:0009792 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0009994 GO:0010165 GO:0010212 GO:0010225 GO:0010332 GO:0010468 GO:0010484 GO:0010485 GO:0010556 GO:0010557 GO:0010558 GO:0010564 GO:0010604 GO:0010605 GO:0010628 GO:0012501 GO:0012505 GO:0015630 GO:0015631 GO:0016043 GO:0016407 GO:0016410 GO:0016569 GO:0016570 GO:0016573 GO:0016740 GO:0016746 GO:0016747 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019538 GO:0019899 GO:0019953 GO:0021700 GO:0022402 GO:0022412 GO:0022414 GO:0023052 GO:0030097 GO:0030141 GO:0030154 GO:0030330 GO:0030879 GO:0031023 GO:0031297 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031410 GO:0031619 GO:0031974 GO:0031981 GO:0031982 GO:0032200 GO:0032465 GO:0032501 GO:0032502 GO:0032504 GO:0032991 GO:0033036 GO:0033260 GO:0033365 GO:0033554 GO:0033593 GO:0033599 GO:0033600 GO:0034212 GO:0034502 GO:0034613 GO:0034641 GO:0034645 GO:0035264 GO:0035556 GO:0036211 GO:0040007 GO:0042127 GO:0042592 GO:0042770 GO:0042771 GO:0042772 GO:0042802 GO:0043009 GO:0043015 GO:0043060 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043543 GO:0043966 GO:0043967 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044454 GO:0044464 GO:0044703 GO:0044786 GO:0045005 GO:0045132 GO:0045137 GO:0045143 GO:0045184 GO:0045787 GO:0045893 GO:0045931 GO:0045934 GO:0045935 GO:0046483 GO:0046545 GO:0046660 GO:0048232 GO:0048285 GO:0048468 GO:0048469 GO:0048477 GO:0048478 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048534 GO:0048589 GO:0048599 GO:0048608 GO:0048609 GO:0048731 GO:0048732 GO:0048856 GO:0048869 GO:0050000 GO:0050678 GO:0050680 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051052 GO:0051053 GO:0051093 GO:0051171 GO:0051172 GO:0051173 GO:0051179 GO:0051234 GO:0051239 GO:0051241 GO:0051252 GO:0051254 GO:0051276 GO:0051298 GO:0051301 GO:0051302 GO:0051303 GO:0051310 GO:0051311 GO:0051316 GO:0051321 GO:0051455 GO:0051640 GO:0051641 GO:0051649 GO:0051656 GO:0051704 GO:0051716 GO:0051726 GO:0060249 GO:0060255 GO:0060322 GO:0061458 GO:0061640 GO:0061733 GO:0061982 GO:0065007 GO:0065008 GO:0070013 GO:0070198 GO:0070199 GO:0070200 GO:0070727 GO:0071695 GO:0071704 GO:0071840 GO:0072331 GO:0072332 GO:0072594 GO:0080090 GO:0090304 GO:0090329 GO:0097159 GO:0097190 GO:0097193 GO:0097708 GO:0098687 GO:0098813 GO:0099086 GO:0099503 GO:0140013 GO:1901360 GO:1901363 GO:1901564 GO:1901576 GO:1902298 GO:1902680 GO:1902969 GO:1903046 GO:1903047 GO:1903506 GO:1903508 GO:1990426 GO:1990505 GO:2000026 GO:2000104 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03440 ko03460 ko05200 ko05212 ko05224 map03440 map03460 map05200 map05212 map05224
Pfam Domains
Protein Families
BRCA-2_OB1 BRCA-2_helical BRCA2

Protein Analysis

1210

Amino Acids

131.78

Weight (kDa)

9.21

Isoelectric Point (pI)

43.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BRCA2 PF00634 222 - 247 5e-06 BRCA2 repeat
BRCA-2_helical PF09169 609 - 679 1.1e-15 BRCA2, helical
BRCA-2_OB1 PF09103 683 - 805 5.1e-41 BRCA2, oligonucleotide/oligosaccharide-binding, domain 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 365, 767
AasI GACNNNNNNGTC 1 cut(s) 464
AbsI CCTCGAGG 1 cut(s) 2330
Acc36I ACCTGC 2 cut(s) 365, 767
Acc65I GGTACC 1 cut(s) 48
AccB1I GGYRCC 3 cut(s) 48, 127, 1478
AccB7I CCANNNNNTGG 3 cut(s) 1852, 1893, 2575
AccI GTMKAC 3 cut(s) 730, 1619, 1793
AccII CGCG 2 cut(s) 1720, 3153
AclWI GGATC 8 cut(s) 423, 436, 608, 1748, 2291, 3073, 3118, 3409
AcsI RAATTY 5 cut(s) 754, 1973, 2724, 3200, 3481
AcuI CTGAAG 1 cut(s) 3489
AdeI CACNNNGTG 1 cut(s) 2303
AfaI GTAC 4 cut(s) 50, 934, 1276, 1995
AfiI CCNNNNNNNGG 9 cut(s) 137, 748, 749, 1365, 1653, 1852, 1893, 2140, 2575
AflII CTTAAG 1 cut(s) 2848
AflIII ACRYGT 2 cut(s) 15, 1734
AgeI ACCGGT 3 cut(s) 42, 2043, 2092
AjnI CCWGG 1 cut(s) 549
AloI GAACNNNNNNTCC 2 cut(s) 2911, 2943
Alw21I GWGCWC 3 cut(s) 23, 2386, 2428
Alw26I GTCTC 4 cut(s) 1990, 2138, 2353, 2558
AlwI GGATC 8 cut(s) 423, 436, 608, 1748, 2291, 3073, 3118, 3409
AlwNI CAGNNNCTG 2 cut(s) 2307, 2714
Ama87I CYCGRG 2 cut(s) 1997, 2330
AoxI GGCC 6 cut(s) 319, 768, 1854, 2315, 2790, 3134
ApoI RAATTY 5 cut(s) 754, 1973, 2724, 3200, 3481
ArsI GACNNNNNNTTYG 6 cut(s) 54, 86, 773, 805, 2262, 2294
AsiGI ACCGGT 3 cut(s) 42, 2043, 2092
Asp700I GAANNNNTTC 3 cut(s) 1192, 1534, 1657
Asp718I GGTACC 1 cut(s) 48
AspA2I CCTAGG 1 cut(s) 1586
AspLEI GCGC 1 cut(s) 2598
AspS9I GGNCC 4 cut(s) 55, 1368, 2315, 2462
AsuC2I CCSGG 2 cut(s) 53, 125
AsuHPI GGTGA 9 cut(s) 749, 791, 1668, 1762, 2033, 2073, 2082, 2316, 3342
AsuII TTCGAA 1 cut(s) 187
AsuNHI GCTAGC 1 cut(s) 2249
AvaI CYCGRG 2 cut(s) 1997, 2330
AvaII GGWCC 3 cut(s) 55, 1368, 2462
AvrII CCTAGG 1 cut(s) 1586
AxyI CCTNAGG 2 cut(s) 316, 3131
BamHI GGATCC 1 cut(s) 428
BanI GGYRCC 3 cut(s) 48, 127, 1478
BanII GRGCYC 1 cut(s) 2386
BarI GAAGNNNNNNTAC 2 cut(s) 1645, 1677
BbsI GAAGAC 1 cut(s) 482
Bbv12I GWGCWC 3 cut(s) 23, 2386, 2428
BccI CCATC 9 cut(s) 886, 950, 1228, 1292, 2189, 2801, 2968, 3029, 3549
BceAI ACGGC 4 cut(s) 115, 236, 2372, 3301
BcgI CGANNNNNNTGC 8 cut(s) 217, 251, 463, 488, 497, 522, 2978, 3012
BciT130I CCWGG 1 cut(s) 551
BclI TGATCA 2 cut(s) 1633, 2395
BcnI CCSGG 2 cut(s) 53, 125
BcoDI GTCTC 4 cut(s) 1990, 2138, 2353, 2558
BfmI CTRYAG 2 cut(s) 2702, 3474
BfoI RGCGCY 1 cut(s) 2599
BfrI CTTAAG 1 cut(s) 2848
BfuAI ACCTGC 2 cut(s) 365, 767
BglII AGATCT 3 cut(s) 686, 2537, 2549
BlnI CCTAGG 1 cut(s) 1586
BlpI GCTNAGC 1 cut(s) 3252
Bme1390I CCNGG 3 cut(s) 53, 125, 551
Bme18I GGWCC 3 cut(s) 55, 1368, 2462
BmeT110I CYCGRG 2 cut(s) 1997, 2330
BmgT120I GGNCC 4 cut(s) 55, 1368, 2315, 2462
BmiI GGNNCC 9 cut(s) 50, 57, 129, 298, 430, 1370, 1480, 2957, 3289
BmrFI CCNGG 3 cut(s) 53, 125, 551
BmtI GCTAGC 1 cut(s) 2253
BpiI GAAGAC 1 cut(s) 482
BplI GAGNNNNNCTC 6 cut(s) 2135, 2167, 2283, 2315, 2673, 2705
BpmI CTGGAG 3 cut(s) 1870, 2175, 3178
Bpu10I CCTNAGC 3 cut(s) 1019, 1361, 2431
Bpu1102I GCTNAGC 1 cut(s) 3252
Bpu14I TTCGAA 1 cut(s) 187
BpuMI CCSGG 2 cut(s) 53, 125
BsaBI GATNNNNATC 2 cut(s) 691, 3569
BsaI GGTCTC 1 cut(s) 2558
BsaJI CCNNGG 4 cut(s) 135, 549, 1586, 2914
BsaWI WCCGGW 4 cut(s) 42, 431, 2043, 2092
BsaXI ACNNNNNCTCC 6 cut(s) 198, 228, 378, 408, 2451, 2481
Bsc4I CCNNNNNNNGG 9 cut(s) 137, 748, 749, 1365, 1653, 1852, 1893, 2140, 2575
Bse118I RCCGGY 5 cut(s) 29, 42, 2043, 2092, 2843
Bse1I ACTGG 7 cut(s) 430, 1171, 1513, 1757, 1935, 2465, 2838
Bse21I CCTNAGG 2 cut(s) 316, 3131
Bse3DI GCAATG 5 cut(s) 479, 893, 973, 1315, 1580
Bse8I GATNNNNATC 2 cut(s) 691, 3569
BseBI CCWGG 1 cut(s) 551
BseDI CCNNGG 4 cut(s) 135, 549, 1586, 2914
BseJI GATNNNNATC 2 cut(s) 691, 3569
BseLI CCNNNNNNNGG 9 cut(s) 137, 748, 749, 1365, 1653, 1852, 1893, 2140, 2575
BseMI GCAATG 5 cut(s) 479, 893, 973, 1315, 1580
BseNI ACTGG 7 cut(s) 430, 1171, 1513, 1757, 1935, 2465, 2838
BseRI GAGGAG 3 cut(s) 1192, 1534, 1561
BseYI CCCAGC 2 cut(s) 2307, 2312
BsgI GTGCAG 3 cut(s) 393, 2345, 2766
Bsh1236I CGCG 2 cut(s) 1720, 3153
Bsh1285I CGRYCG 1 cut(s) 914
BshFI GGCC 6 cut(s) 321, 770, 1856, 2317, 2792, 3136
BshNI GGYRCC 3 cut(s) 48, 127, 1478
BshTI ACCGGT 3 cut(s) 42, 2043, 2092
BsiEI CGRYCG 1 cut(s) 914
BsiHKAI GWGCWC 3 cut(s) 23, 2386, 2428
BsiHKCI CYCGRG 2 cut(s) 1997, 2330
BsiSI CCGG 8 cut(s) 30, 43, 53, 124, 432, 2044, 2093, 2844
BslFI GGGAC 2 cut(s) 574, 2453
BslI CCNNNNNNNGG 9 cut(s) 137, 748, 749, 1365, 1653, 1852, 1893, 2140, 2575
BsmAI GTCTC 4 cut(s) 1990, 2138, 2353, 2558
BsmFI GGGAC 2 cut(s) 574, 2453
BsmI GAATGC 2 cut(s) 1098, 1719
BsnI GGCC 6 cut(s) 321, 770, 1856, 2317, 2792, 3136
Bso31I GGTCTC 1 cut(s) 2558
BsoBI CYCGRG 2 cut(s) 1997, 2330
Bsp119I TTCGAA 1 cut(s) 187
Bsp1286I GDGCHC 3 cut(s) 23, 2386, 2428
Bsp1720I GCTNAGC 1 cut(s) 3252
Bsp19I CCATGG 1 cut(s) 135
Bsp68I TCGCGA 1 cut(s) 3153
BspANI GGCC 6 cut(s) 321, 770, 1856, 2317, 2792, 3136
BspFNI CGCG 2 cut(s) 1720, 3153
BspHI TCATGA 1 cut(s) 2874
BspLI GGNNCC 9 cut(s) 50, 57, 129, 298, 430, 1370, 1480, 2957, 3289
BspMI ACCTGC 2 cut(s) 365, 767
BspOI GCTAGC 1 cut(s) 2253
BspPI GGATC 8 cut(s) 423, 436, 608, 1748, 2291, 3073, 3118, 3409
BspT104I TTCGAA 1 cut(s) 187
BspT107I GGYRCC 3 cut(s) 48, 127, 1478
BspTI CTTAAG 1 cut(s) 2848
BspTNI GGTCTC 1 cut(s) 2558
BsrDI GCAATG 5 cut(s) 479, 893, 973, 1315, 1580
BsrFI RCCGGY 5 cut(s) 29, 42, 2043, 2092, 2843
BsrI ACTGG 7 cut(s) 430, 1171, 1513, 1757, 1935, 2465, 2838
BssAI RCCGGY 5 cut(s) 29, 42, 2043, 2092, 2843
BssECI CCNNGG 4 cut(s) 135, 549, 1586, 2914
BssNAI GTATAC 1 cut(s) 1794
BssT1I CCWWGG 3 cut(s) 135, 1586, 2914
Bst1107I GTATAC 1 cut(s) 1794
Bst2UI CCWGG 1 cut(s) 551
Bst6I CTCTTC 1 cut(s) 3352
BstAFI CTTAAG 1 cut(s) 2848
BstAPI GCANNNNNTGC 2 cut(s) 895, 1781
BstBI TTCGAA 1 cut(s) 187
BstDSI CCRYGG 1 cut(s) 135
BstEII GGTNACC 2 cut(s) 737, 2039
BstFNI CGCG 2 cut(s) 1720, 3153
BstH2I RGCGCY 1 cut(s) 2599
BstHHI GCGC 1 cut(s) 2598
BstMAI GTCTC 4 cut(s) 1990, 2138, 2353, 2558
BstMCI CGRYCG 1 cut(s) 914
BstMWI GCNNNNNNNGC 9 cut(s) 120, 554, 895, 1447, 1781, 1821, 2390, 2432, 3245
BstNI CCWGG 1 cut(s) 551
BstNSI RCATGY 3 cut(s) 19, 1738, 1852
BstPI GGTNACC 2 cut(s) 737, 2039
BstSCI CCNGG 3 cut(s) 51, 123, 549
BstSFI CTRYAG 2 cut(s) 2702, 3474
BstUI CGCG 2 cut(s) 1720, 3153
BstV2I GAAGAC 1 cut(s) 482
BstX2I RGATCY 8 cut(s) 428, 686, 1753, 2283, 2537, 2549, 3123, 3401
BstXI CCANNNNNNTGG 3 cut(s) 523, 1298, 1453
BstYI RGATCY 8 cut(s) 428, 686, 1753, 2283, 2537, 2549, 3123, 3401
BstZ17I GTATAC 1 cut(s) 1794
Bsu36I CCTNAGG 2 cut(s) 316, 3131
BsuRI GGCC 6 cut(s) 321, 770, 1856, 2317, 2792, 3136
BtgI CCRYGG 1 cut(s) 135
BtgZI GCGATG 1 cut(s) 2685
BtsI GCAGTG 4 cut(s) 590, 884, 1226, 1482
BtuMI TCGCGA 1 cut(s) 3153
BveI ACCTGC 2 cut(s) 365, 767
CaiI CAGNNNCTG 2 cut(s) 2307, 2714
CciI TCATGA 1 cut(s) 2874
CfoI GCGC 1 cut(s) 2598
Cfr10I RCCGGY 5 cut(s) 29, 42, 2043, 2092, 2843
Cfr13I GGNCC 4 cut(s) 55, 1368, 2315, 2462
Csp6I GTAC 4 cut(s) 49, 933, 1275, 1994
CspAI ACCGGT 3 cut(s) 42, 2043, 2092
CspCI CAANNNNNGTGG 4 cut(s) 502, 537, 2437, 2472
CviQI GTAC 4 cut(s) 49, 933, 1275, 1994
DraI TTTAAA 2 cut(s) 1173, 3048
DraIII CACNNNGTG 1 cut(s) 2303
DrdI GACNNNNNNGTC 1 cut(s) 464
DseDI GACNNNNNNGTC 1 cut(s) 464
Eam1104I CTCTTC 1 cut(s) 3352
EarI CTCTTC 1 cut(s) 3352
EciI GGCGGA 3 cut(s) 188, 381, 399
Ecl136II GAGCTC 1 cut(s) 2384
Eco130I CCWWGG 3 cut(s) 135, 1586, 2914
Eco24I GRGCYC 1 cut(s) 2386
Eco31I GGTCTC 1 cut(s) 2558
Eco32I GATATC 1 cut(s) 1670
Eco47I GGWCC 3 cut(s) 55, 1368, 2462
Eco53kI GAGCTC 1 cut(s) 2384
Eco57I CTGAAG 1 cut(s) 3489
Eco81I CCTNAGG 2 cut(s) 316, 3131
Eco88I CYCGRG 2 cut(s) 1997, 2330
Eco91I GGTNACC 2 cut(s) 737, 2039
EcoICRI GAGCTC 1 cut(s) 2384
EcoO109I RGGNCCY 1 cut(s) 1368
EcoO65I GGTNACC 2 cut(s) 737, 2039
EcoRII CCWGG 1 cut(s) 549
EcoRV GATATC 1 cut(s) 1670
EcoT14I CCWWGG 3 cut(s) 135, 1586, 2914
EcoT22I ATGCAT 2 cut(s) 2080, 2108
EcoT38I GRGCYC 1 cut(s) 2386
ErhI CCWWGG 3 cut(s) 135, 1586, 2914
FalI AAGNNNNNCTT 4 cut(s) 1691, 1723, 2172, 2204
FaqI GGGAC 2 cut(s) 574, 2453
FbaI TGATCA 2 cut(s) 1633, 2395
FblI GTMKAC 3 cut(s) 730, 1619, 1793
FriOI GRGCYC 1 cut(s) 2386
GlaI GCGC 1 cut(s) 2597
GsaI CCCAGC 2 cut(s) 2311, 2316
GsuI CTGGAG 3 cut(s) 1870, 2175, 3178
HaeII RGCGCY 1 cut(s) 2599
HaeIII GGCC 6 cut(s) 321, 770, 1856, 2317, 2792, 3136
HapII CCGG 8 cut(s) 30, 43, 53, 124, 432, 2044, 2093, 2844
HhaI GCGC 1 cut(s) 2598
Hin6I GCGC 1 cut(s) 2596
HinP1I GCGC 1 cut(s) 2596
HincII GTYRAC 1 cut(s) 1260
HindII GTYRAC 1 cut(s) 1260
HindIII AAGCTT 2 cut(s) 1836, 2276
HpaII CCGG 8 cut(s) 30, 43, 53, 124, 432, 2044, 2093, 2844
HphI GGTGA 9 cut(s) 749, 791, 1668, 1762, 2033, 2073, 2082, 2316, 3342
Hpy166II GTNNAC 6 cut(s) 731, 812, 1260, 1620, 1794, 1846
Hpy8I GTNNAC 6 cut(s) 731, 812, 1260, 1620, 1794, 1846
Hpy99I CGWCG 1 cut(s) 251
HpyAV CCTTC 9 cut(s) 663, 1647, 1683, 2063, 2094, 2606, 2672, 2930, 3305
HpyCH4IV ACGT 3 cut(s) 718, 1796, 3514
HpyF10VI GCNNNNNNNGC 9 cut(s) 120, 554, 895, 1447, 1781, 1821, 2390, 2432, 3245
HpySE526I ACGT 3 cut(s) 718, 1796, 3514
HspAI GCGC 1 cut(s) 2596
KpnI GGTACC 1 cut(s) 52
KroI GCCGGC 2 cut(s) 29, 2843
KroNI GCCGGC 2 cut(s) 31, 2845
Ksp22I TGATCA 2 cut(s) 1633, 2395
MaeII ACGT 3 cut(s) 718, 1796, 3514
MaeIII GTNAC 9 cut(s) 737, 779, 1338, 1681, 1897, 2039, 2129, 2863, 3277
MfeI CAATTG 5 cut(s) 905, 1048, 1247, 1390, 2399
MflI RGATCY 8 cut(s) 428, 686, 1753, 2283, 2537, 2549, 3123, 3401
MhlI GDGCHC 3 cut(s) 23, 2386, 2428
MlyI GAGTC 4 cut(s) 556, 736, 1817, 2496
MmeI TCCRAC 7 cut(s) 1069, 1719, 2248, 2541, 2784, 2872, 3288
Mph1103I ATGCAT 2 cut(s) 2080, 2108
MroNI GCCGGC 2 cut(s) 29, 2843
MroXI GAANNNNTTC 3 cut(s) 1192, 1534, 1657
MslI CAYNNNNRTG 3 cut(s) 1487, 2440, 2879
MspA1I CMGCKG 1 cut(s) 2714
MspCI CTTAAG 1 cut(s) 2848
MspI CCGG 8 cut(s) 30, 43, 53, 124, 432, 2044, 2093, 2844
MspR9I CCNGG 3 cut(s) 53, 125, 551
MunI CAATTG 5 cut(s) 905, 1048, 1247, 1390, 2399
Mva1269I GAATGC 2 cut(s) 1098, 1719
MvaI CCWGG 1 cut(s) 551
MvnI CGCG 2 cut(s) 1720, 3153
MwoI GCNNNNNNNGC 9 cut(s) 120, 554, 895, 1447, 1781, 1821, 2390, 2432, 3245
NaeI GCCGGC 2 cut(s) 31, 2845
NciI CCSGG 2 cut(s) 53, 125
NcoI CCATGG 1 cut(s) 135
NgoMIV GCCGGC 2 cut(s) 29, 2843
NheI GCTAGC 1 cut(s) 2249
NlaIV GGNNCC 9 cut(s) 50, 57, 129, 298, 430, 1370, 1480, 2957, 3289
NmuCI GTSAC 7 cut(s) 737, 779, 1681, 1897, 2039, 2129, 2863
NruI TCGCGA 1 cut(s) 3153
NsiI ATGCAT 2 cut(s) 2080, 2108
NspI RCATGY 3 cut(s) 19, 1738, 1852
NspV TTCGAA 1 cut(s) 187
PaeR7I CTCGAG 2 cut(s) 1997, 2330
PagI TCATGA 1 cut(s) 2874
PaqCI CACCTGC 2 cut(s) 365, 767
PciI ACATGT 2 cut(s) 15, 1734
PcsI WCGNNNNNNNCGW 1 cut(s) 243
PctI GAATGC 2 cut(s) 1098, 1719
PdiI GCCGGC 2 cut(s) 31, 2845
PdmI GAANNNNTTC 3 cut(s) 1192, 1534, 1657
PflMI CCANNNNNTGG 3 cut(s) 1852, 1893, 2575
PinAI ACCGGT 3 cut(s) 42, 2043, 2092
PleI GAGTC 4 cut(s) 556, 735, 1816, 2495
PpsI GAGTC 4 cut(s) 556, 735, 1816, 2495
PpuMI RGGWCCY 1 cut(s) 1368
PscI ACATGT 2 cut(s) 15, 1734
Psp124BI GAGCTC 1 cut(s) 2386
Psp5II RGGWCCY 1 cut(s) 1368
Psp6I CCWGG 1 cut(s) 549
PspEI GGTNACC 2 cut(s) 737, 2039
PspFI CCCAGC 2 cut(s) 2307, 2312
PspGI CCWGG 1 cut(s) 549
PspN4I GGNNCC 9 cut(s) 50, 57, 129, 298, 430, 1370, 1480, 2957, 3289
PspPI GGNCC 4 cut(s) 55, 1368, 2315, 2462
PspPPI RGGWCCY 1 cut(s) 1368
PspXI VCTCGAGB 2 cut(s) 1997, 2330
PstNI CAGNNNCTG 2 cut(s) 2307, 2714
PsuI RGATCY 8 cut(s) 428, 686, 1753, 2283, 2537, 2549, 3123, 3401
PvuII CAGCTG 1 cut(s) 2714
RruI TCGCGA 1 cut(s) 3153
RsaI GTAC 4 cut(s) 50, 934, 1276, 1995
RsaNI GTAC 4 cut(s) 49, 933, 1275, 1994
RseI CAYNNNNRTG 3 cut(s) 1487, 2440, 2879
SacI GAGCTC 1 cut(s) 2386
Sau96I GGNCC 4 cut(s) 55, 1368, 2315, 2462
SchI GAGTC 4 cut(s) 556, 736, 1817, 2496
ScrFI CCNGG 3 cut(s) 53, 125, 551
SduI GDGCHC 3 cut(s) 23, 2386, 2428
SfcI CTRYAG 2 cut(s) 2702, 3474
Sfr274I CTCGAG 2 cut(s) 1997, 2330
SfuI TTCGAA 1 cut(s) 187
SgrAI CRCCGGYG 1 cut(s) 2092
SinI GGWCC 3 cut(s) 55, 1368, 2462
SlaI CTCGAG 2 cut(s) 1997, 2330
SmiMI CAYNNNNRTG 3 cut(s) 1487, 2440, 2879
SmlI CTYRAG 3 cut(s) 1997, 2330, 2848
SmoI CTYRAG 3 cut(s) 1997, 2330, 2848
SstI GAGCTC 1 cut(s) 2386
StyD4I CCNGG 3 cut(s) 51, 123, 549
StyI CCWWGG 3 cut(s) 135, 1586, 2914
TaiI ACGT 3 cut(s) 721, 1799, 3517
TaqI TCGA 9 cut(s) 187, 954, 1296, 1998, 2184, 2223, 2331, 2988, 3114
TatI WGTACW 1 cut(s) 1993
TauI GCSGC 2 cut(s) 1441, 1720
TseFI GTSAC 7 cut(s) 737, 779, 1681, 1897, 2039, 2129, 2863
Tsp45I GTSAC 7 cut(s) 737, 779, 1681, 1897, 2039, 2129, 2863
TspGWI ACGGA 4 cut(s) 246, 2385, 2509, 3208
Van91I CCANNNNNTGG 3 cut(s) 1852, 1893, 2575
Vha464I CTTAAG 1 cut(s) 2848
VpaK11BI GGWCC 3 cut(s) 55, 1368, 2462
XapI RAATTY 5 cut(s) 754, 1973, 2724, 3200, 3481
XceI RCATGY 3 cut(s) 19, 1738, 1852
XcmI CCANNNNNNNNNTGG 1 cut(s) 520
XhoI CTCGAG 2 cut(s) 1997, 2330
XmaJI CCTAGG 1 cut(s) 1586
XmiI GTMKAC 3 cut(s) 730, 1619, 1793
XmnI GAANNNNTTC 3 cut(s) 1192, 1534, 1657
Zsp2I ATGCAT 2 cut(s) 2080, 2108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.