Prupe.4G287200_v2.0.a1

BRCA2, oligonucleotide/oligosaccharide-binding, domain 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0004075
Physical Location & Seq
Reverse (-)
25170678 .. 25179683
9006 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G287200.7

Sequence Viewer

Length: 3312 bp
ATGTCGACGTGGCAGATGTTCGCCGACGCCGGCAACAATTACCGGTGGTATCCCGACACACCAAACGGCGCCGTTTATCCTACTCCTACTACTACTACTACTACTACTCTTCATAGTGATAGTGAAAGGCAGCAGCAGCTCAAGTCGAGCAGTCGGCTGCCTTCCATGGCGGATCTGCTGCTTCAAGGTTGTTCGAAGCTTGCAGAGGCCCAGACTCAGAATCAGAGAAATGGCTTTGATGCTGATGACGGTGTTGGGATGTTTCGCAACGGATTTGGAAGACCCGTCGCCATCAAGCCATCCTCCTTAGCCAAAGCGTCGTCGCTTCTTCAAACTGGAACTGGCCAAGTGCAAGCTACGAATAGTAGAGGTGGTTTCTCTAATTCCCTTTTCCAGACTGGCTCTGGGAAAATGGTTAATATATCTCCGGACGGTCTTGTGAGGGCAAAGACATTGCTTGGTTTGGGAGACGACAATGATCACAGCAAGCTTCCGGGTTCCAACAGTGGTGGGGTGGCTATGGATGCTGCATCCATTTCAAGGTCTCCGTTAATTAATAAGACTGTTTCAGTACAAACTAGATGTAAGAAGAATGAGGCTGATCTAAATTTTATGTCGCCTGAAAGGCTTAATTTAACTCCGGACAAACCTTCATCAATCAAGTTCCATACAGCTGGAGGAAGATCTATATCTGTTTCTACTGATGCACTGCAACGTGCAAGGAGTCTACTTGGTGACCCAGAGTTAGGGAGTTTCTTGAATGAGGGGGATGCAGGTGACTCCGTTTTCTCATTTTCAAAAGGTAGAGGACATGATGAACGCACTCCCTTCTCCCATCAGAAAATGACTAGGAAAAACTTTTTAACTAAGACTTCTGTATCTCCACTGCAATCATCATCTAAGCAAGTGCGGTCATCGTCGGCCATAAATTCCAGCACTAATCTGATTACACAATTTGATGTTGTTAGCAATGAAAGTGTCTGTAAGTCAAACAGTGAGTTACCTTATCGGCAGGAGAAGCCCCTTAGCGACAAACCTTGTATCATCAAGACAGTAGAAAACAATTATTTGGAAAATGGTGGTAGTTTGAGGATTAATCCTGTTGGAAAGTCACTAGCCAAGCCATTGGTTGATATTTCAAATACCGTTGGCACCACTGCTATGAATAGCACACAAAAGAGTGGTGTAAAGAGGAGGCTTGGAAGAAGTTCTATTTCTCCATTCAAGAAGCCTCGCACTTCCAACTCCTCCACTCCATTGCACAAGAATGTTCCTCTTGTCCGTAATGGTTTGTCTACTTTGTCCTCCGACCATTTGTGTTCCAAAAGAAGGGTTTCTACTAGATATCCTTTCTCAGTAACTAGAATGTATGTGAAGAAATATTTTGGAATGCCACCACCTGACCAGAACATGTTTGAGTGTTTGTCAGACCCGGGGAGAAGAATTACAGCAAGTAACGCAGAAAAGTACATGTTTCTTGATGAATCTGGCTTAAACTGCATAGGAGCAGAAGCTTTTGTCCACATGTTGGCTCGTTCTGGAGCTTTAATGCAATATACTTCCAGAGAGTGGGTCACAAATCACTACAAGTGGGTTGTCTGGAAACTGGCTTGCTATGAGAGATGTCATCTAGCTAAGTCGTTCGGAAATTTTTTGACAGTCTCCAATGTGCTTGAGGAATTGAAGTATAGATATGAAAGAGAAGTGAATCATGGTCACCGATCTGCAATTAAGAGGATTCTGGAAGGAGATGCATCCCCTTCTTCAATGGTGGTTCTATGCATTTCAGCTATTCGTTCAAATTGTGACCCAAATATGGAGACTAGCTCCAGAGCTGAAAATAGTGGTGCTGCAAAAGTTGAGCTTACTGATGGGTGGTATTCAGTGGATGCTGTACTGGATGCACTCCTGTCGAAGCAGCTTGCTAGTGGAAAATTGTTTGTTGGACAGAAACTTCGGATCTGGGGAGCAGCACTCTGTGGTTGGGCTGGGCCTGTTTCACCCCTTGAGGTTTCTGCTTCAACTCAGGTGGCAAGAACAGTTACTTTCCGGCTGCACATAAATGGAACATATAGAGCTCATTGGGCTGATCGATTGGGATTTTGTAAGGGTGCTGGTGCTCCCTTAGCATTTAACTGTGTCAAGAGTGGTGGTGGTGCAGTCCCTTGCACTCTTCTAGGAGTCACACGGATATACCCTGTTCTCTACAAAGAGAGGTTAAGTAATGGAAGATCTGTTGTAAGATCAGAGAGGTTGGAGTCCCAAATGGTGCAATCATACCAAGAGAGGCGCTCTAATGTCATAGAAGGCATTATATCTGAGTTCCAAAGAGGACTAGAACATTCACATTTGTGTAATGATAGCGATAGTGAAGGAGCAAAACTCTTAAAGATTCTAGAAACAGCCACTGAACCTGAAATTTTAATGGCAGAAATGAGTTCAGAACAGTTGAAATCTTTTACCAAATATCGATCAAAGCTGGAGGCAATTAAGCAGTCAGACATGGAGAAATCAATTTTGAAAGCACTGGAAGATGCCGGCTTAAGTGAGAGGGAAGTCACGCCATTCATGAGGGTGAGGGTGGTTGGACTAACAAGAAAATTATGTCATGGGAAAGACAGTTCTAAAGAAGGCCTAATAACAATCTGGAACCCATCTGAGAAGCAGCAGAAGACTGAGCTGGTTGAGGGACGTGCATATCGTGTTTCTGGACTTATACCAACAAGTTCTGATGTGGGTACTCTACACTTGCTAGTGAGAGGATCCACAACAACATGGCAGCCTTTATCTCAGCAGGCAGTCGAACACTTCAAGCCTTTTTTCAATCCTCGCAAATCAGTCTTATTGTCAGATTTGGGTAAAGTTCCTCTTTCCAGTGAATTTGATATTGCTGCATTCGTTGTGTTTGTGGGGGAGGTTTATATAGCTGCTCACCAGAAGAAACAGTGGGTGTTCGTGACAGATGGCTCCATATCTGAATTAAATTCAGAAGAATCATGTGATTCTCTACTTGCCGTCTGCTTCTGTTCACCATACACTGGCGTTGATTCAATTGCTCCTATAAACTATAACCTCACAGGATCAACGGTTGGGTTTTGTAATCTTACCAAGAGAGAAAAGGACCAAATGAATTCCCTTTGGGTAGCAGAAGCTACAGAAACTTCAGCTTACTTTTTAAGTTTTGACACTCCACATTGTTCTCATCTCAAAGATGCTGCTGTCTCCACAGAAAGATGGGGAAGGATCTCTAGCTTGACTATCAATAGGCTTAAGGAGAGGGTTTTGTGTATTATTGGTGCTAGCAAAGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000226 GO:0000228 GO:0000278 GO:0000280 GO:0000281 GO:0000722 GO:0000723 GO:0000724 GO:0000725 GO:0000781 GO:0000784 GO:0000793 GO:0000794 GO:0000795 GO:0000800 GO:0000910 GO:0001556 GO:0001701 GO:0001824 GO:0001832 GO:0001833 GO:0002020 GO:0002376 GO:0002520 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003697 GO:0003824 GO:0004402 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005739 GO:0005813 GO:0005815 GO:0005829 GO:0005856 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006275 GO:0006281 GO:0006289 GO:0006302 GO:0006310 GO:0006312 GO:0006325 GO:0006355 GO:0006464 GO:0006473 GO:0006475 GO:0006725 GO:0006807 GO:0006915 GO:0006950 GO:0006974 GO:0006978 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007059 GO:0007098 GO:0007127 GO:0007140 GO:0007141 GO:0007154 GO:0007165 GO:0007275 GO:0007276 GO:0007281 GO:0007283 GO:0007292 GO:0007346 GO:0007399 GO:0007417 GO:0007420 GO:0007548 GO:0007568 GO:0007569 GO:0008022 GO:0008080 GO:0008092 GO:0008104 GO:0008150 GO:0008152 GO:0008156 GO:0008219 GO:0008283 GO:0008285 GO:0008406 GO:0008585 GO:0008608 GO:0008630 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009790 GO:0009792 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0009994 GO:0010165 GO:0010212 GO:0010225 GO:0010332 GO:0010468 GO:0010484 GO:0010485 GO:0010556 GO:0010557 GO:0010558 GO:0010564 GO:0010604 GO:0010605 GO:0010628 GO:0012501 GO:0012505 GO:0015630 GO:0015631 GO:0016043 GO:0016407 GO:0016410 GO:0016569 GO:0016570 GO:0016573 GO:0016740 GO:0016746 GO:0016747 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019538 GO:0019899 GO:0019953 GO:0021700 GO:0022402 GO:0022412 GO:0022414 GO:0023052 GO:0030097 GO:0030141 GO:0030154 GO:0030330 GO:0030879 GO:0031023 GO:0031297 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031410 GO:0031619 GO:0031974 GO:0031981 GO:0031982 GO:0032200 GO:0032465 GO:0032501 GO:0032502 GO:0032504 GO:0032991 GO:0033036 GO:0033260 GO:0033365 GO:0033554 GO:0033593 GO:0033599 GO:0033600 GO:0034212 GO:0034502 GO:0034613 GO:0034641 GO:0034645 GO:0035264 GO:0035556 GO:0036211 GO:0040007 GO:0042127 GO:0042592 GO:0042770 GO:0042771 GO:0042772 GO:0042802 GO:0043009 GO:0043015 GO:0043060 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043543 GO:0043966 GO:0043967 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044454 GO:0044464 GO:0044703 GO:0044786 GO:0045005 GO:0045132 GO:0045137 GO:0045143 GO:0045184 GO:0045787 GO:0045893 GO:0045931 GO:0045934 GO:0045935 GO:0046483 GO:0046545 GO:0046660 GO:0048232 GO:0048285 GO:0048468 GO:0048469 GO:0048477 GO:0048478 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048534 GO:0048589 GO:0048599 GO:0048608 GO:0048609 GO:0048731 GO:0048732 GO:0048856 GO:0048869 GO:0050000 GO:0050678 GO:0050680 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051052 GO:0051053 GO:0051093 GO:0051171 GO:0051172 GO:0051173 GO:0051179 GO:0051234 GO:0051239 GO:0051241 GO:0051252 GO:0051254 GO:0051276 GO:0051298 GO:0051301 GO:0051302 GO:0051303 GO:0051310 GO:0051311 GO:0051316 GO:0051321 GO:0051455 GO:0051640 GO:0051641 GO:0051649 GO:0051656 GO:0051704 GO:0051716 GO:0051726 GO:0060249 GO:0060255 GO:0060322 GO:0061458 GO:0061640 GO:0061733 GO:0061982 GO:0065007 GO:0065008 GO:0070013 GO:0070198 GO:0070199 GO:0070200 GO:0070727 GO:0071695 GO:0071704 GO:0071840 GO:0072331 GO:0072332 GO:0072594 GO:0080090 GO:0090304 GO:0090329 GO:0097159 GO:0097190 GO:0097193 GO:0097708 GO:0098687 GO:0098813 GO:0099086 GO:0099503 GO:0140013 GO:1901360 GO:1901363 GO:1901564 GO:1901576 GO:1902298 GO:1902680 GO:1902969 GO:1903046 GO:1903047 GO:1903506 GO:1903508 GO:1990426 GO:1990505 GO:2000026 GO:2000104 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03440 ko03460 ko05200 ko05212 ko05224 map03440 map03460 map05200 map05212 map05224
Pfam Domains
Protein Families
BRCA-2_OB1 BRCA-2_helical BRCA2

Protein Analysis

1104

Amino Acids

121.11

Weight (kDa)

9.21

Isoelectric Point (pI)

39.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 764
Acc36I ACCTGC 1 cut(s) 764
AccB1I GGYRCC 2 cut(s) 68, 1151
AccB7I CCANNNNNTGG 3 cut(s) 1528, 1569, 3038
AccI GTMKAC 3 cut(s) 5, 727, 1295
AccIII TCCGGA 2 cut(s) 427, 640
AciI CCGC 2 cut(s) 170, 910
AclWI GGATC 6 cut(s) 180, 1967, 2757, 2770, 3088, 3251
AcoI YGGCCR 2 cut(s) 343, 921
AcsI RAATTY 7 cut(s) 607, 928, 1648, 2418, 2879, 2983, 3130
AcuI CTGAAG 1 cut(s) 3147
AcyI GRCGYC 2 cut(s) 27, 69
AdeI CACNNNGTG 1 cut(s) 1979
AfaI GTAC 4 cut(s) 573, 1469, 1896, 2740
AfiI CCNNNNNNNGG 7 cut(s) 540, 746, 1329, 1528, 1569, 1816, 3038
AflII CTTAAG 2 cut(s) 2542, 3269
AflIII ACRYGT 3 cut(s) 1410, 1470, 1524
AgeI ACCGGT 1 cut(s) 42
AjiI CACGTC 2 cut(s) 9, 2693
AleI CACNNNNGTG 1 cut(s) 2350
AloI GAACNNNNNNTCC 2 cut(s) 2605, 2637
Alw21I GWGCWC 2 cut(s) 2080, 2122
Alw26I GTCTC 5 cut(s) 462, 549, 1666, 1814, 3226
AlwI GGATC 6 cut(s) 180, 1967, 2757, 2770, 3088, 3251
AlwNI CAGNNNCTG 1 cut(s) 2408
Ama87I CYCGRG 1 cut(s) 1432
Aor13HI TCCGGA 2 cut(s) 427, 640
AoxI GGCC 5 cut(s) 207, 343, 921, 1991, 2632
ApoI RAATTY 7 cut(s) 607, 928, 1648, 2418, 2879, 2983, 3130
ArsI GACNNNNNNTTYG 2 cut(s) 1938, 1970
AseI ATTAAT 2 cut(s) 555, 1095
AsiGI ACCGGT 1 cut(s) 42
Asp700I GAANNNNTTC 2 cut(s) 1207, 1333
AspLEI GCGC 2 cut(s) 71, 2292
AspS9I GGNCC 3 cut(s) 208, 1991, 3121
AsuC2I CCSGG 3 cut(s) 495, 1433, 1434
AsuHPI GGTGA 7 cut(s) 746, 788, 1709, 1992, 2587, 2924, 3021
AsuII TTCGAA 1 cut(s) 194
AsuNHI GCTAGC 1 cut(s) 3299
AvaI CYCGRG 1 cut(s) 1432
AvaII GGWCC 1 cut(s) 3121
BaeI ACNNNNGTAYC 2 cut(s) 861, 894
BalI TGGCCA 1 cut(s) 345
BamHI GGATCC 1 cut(s) 2762
BanI GGYRCC 2 cut(s) 68, 1151
BanII GRGCYC 1 cut(s) 2080
BarI GAAGNNNNNNTAC 2 cut(s) 1321, 1353
BbsI GAAGAC 2 cut(s) 286, 2678
Bbv12I GWGCWC 2 cut(s) 2080, 2122
BccI CCATC 7 cut(s) 299, 307, 843, 1865, 2662, 2957, 3228
BceAI ACGGC 3 cut(s) 56, 82, 2999
BcgI CGANNNNNNTGC 2 cut(s) 1893, 1927
BciVI GTATCC 1 cut(s) 60
BclI TGATCA 1 cut(s) 478
BcnI CCSGG 3 cut(s) 495, 1433, 1434
BcoDI GTCTC 5 cut(s) 462, 549, 1666, 1814, 3226
BfmI CTRYAG 1 cut(s) 3153
BfoI RGCGCY 2 cut(s) 72, 2293
BfrI CTTAAG 2 cut(s) 2542, 3269
BfuAI ACCTGC 1 cut(s) 764
BfuI GTATCC 1 cut(s) 60
BglI GCCNNNNNGGC 1 cut(s) 625
BglII AGATCT 2 cut(s) 683, 2231
Bme1390I CCNGG 3 cut(s) 495, 1433, 1434
Bme18I GGWCC 1 cut(s) 3121
BmeT110I CYCGRG 1 cut(s) 1432
BmgBI CACGTC 2 cut(s) 9, 2693
BmgT120I GGNCC 3 cut(s) 208, 1991, 3121
BmiI GGNNCC 6 cut(s) 70, 499, 1153, 2651, 2764, 2968
BmrFI CCNGG 3 cut(s) 495, 1433, 1434
BmtI GCTAGC 1 cut(s) 3303
BpiI GAAGAC 2 cut(s) 286, 2678
BplI GAGNNNNNCTC 8 cut(s) 1811, 1843, 1959, 1991, 2276, 2308, 2367, 2399
BpmI CTGGAG 4 cut(s) 696, 1560, 1813, 2501
Bpu10I CCTNAGC 3 cut(s) 307, 1025, 2125
Bpu14I TTCGAA 1 cut(s) 194
BpuEI CTTGAG 3 cut(s) 125, 1694, 2027
BpuMI CCSGG 3 cut(s) 495, 1433, 1434
Bsa29I ATCGAT 2 cut(s) 2092, 2470
BsaBI GATNNNNATC 1 cut(s) 688
BsaHI GRCGYC 2 cut(s) 27, 69
BsaI GGTCTC 1 cut(s) 549
BsaJI CCNNGG 3 cut(s) 165, 1432, 1433
BsaWI WCCGGW 3 cut(s) 42, 427, 640
Bsc4I CCNNNNNNNGG 7 cut(s) 540, 746, 1329, 1528, 1569, 1816, 3038
Bse118I RCCGGY 3 cut(s) 29, 42, 2537
Bse1I ACTGG 8 cut(s) 340, 346, 403, 1611, 1902, 2532, 2874, 3043
Bse3DI GCAATG 3 cut(s) 452, 976, 1256
Bse8I GATNNNNATC 1 cut(s) 688
BseAI TCCGGA 2 cut(s) 427, 640
BseCI ATCGAT 2 cut(s) 2092, 2470
BseDI CCNNGG 3 cut(s) 165, 1432, 1433
BseGI GGATG 8 cut(s) 264, 299, 529, 530, 775, 1754, 1894, 1906
BseJI GATNNNNATC 1 cut(s) 688
BseLI CCNNNNNNNGG 7 cut(s) 540, 746, 1329, 1528, 1569, 1816, 3038
BseMI GCAATG 3 cut(s) 452, 976, 1256
BseMII CTCAG 7 cut(s) 230, 1368, 2039, 2310, 2649, 2667, 2804
BseNI ACTGG 8 cut(s) 340, 346, 403, 1611, 1902, 2532, 2874, 3043
BseRI GAGGAG 2 cut(s) 1207, 1237
BseYI CCCAGC 1 cut(s) 1988
BsgI GTGCAG 2 cut(s) 2039, 2178
BshFI GGCC 5 cut(s) 209, 345, 923, 1993, 2634
BshNI GGYRCC 2 cut(s) 68, 1151
BshTI ACCGGT 1 cut(s) 42
BshVI ATCGAT 2 cut(s) 2092, 2470
BsiHKAI GWGCWC 2 cut(s) 2080, 2122
BsiHKCI CYCGRG 1 cut(s) 1432
BsiSI CCGG 8 cut(s) 30, 43, 428, 494, 641, 1433, 2050, 2538
BslFI GGGAC 3 cut(s) 2147, 2245, 2703
BslI CCNNNNNNNGG 7 cut(s) 540, 746, 1329, 1528, 1569, 1816, 3038
BsmAI GTCTC 5 cut(s) 462, 549, 1666, 1814, 3226
BsmBI CGTCTC 1 cut(s) 462
BsmFI GGGAC 3 cut(s) 2147, 2245, 2703
BsmI GAATGC 2 cut(s) 1395, 2894
BsnI GGCC 5 cut(s) 209, 345, 923, 1993, 2634
Bso31I GGTCTC 1 cut(s) 549
BsoBI CYCGRG 1 cut(s) 1432
Bsp119I TTCGAA 1 cut(s) 194
Bsp1286I GDGCHC 2 cut(s) 2080, 2122
Bsp13I TCCGGA 2 cut(s) 427, 640
Bsp19I CCATGG 1 cut(s) 165
BspACI CCGC 2 cut(s) 170, 910
BspANI GGCC 5 cut(s) 209, 345, 923, 1993, 2634
BspCNI CTCAG 7 cut(s) 229, 1367, 2038, 2311, 2650, 2668, 2803
BspDI ATCGAT 2 cut(s) 2092, 2470
BspEI TCCGGA 2 cut(s) 427, 640
BspHI TCATGA 1 cut(s) 2568
BspLI GGNNCC 6 cut(s) 70, 499, 1153, 2651, 2764, 2968
BspMI ACCTGC 1 cut(s) 764
BspOI GCTAGC 1 cut(s) 3303
BspPI GGATC 6 cut(s) 180, 1967, 2757, 2770, 3088, 3251
BspT104I TTCGAA 1 cut(s) 194
BspT107I GGYRCC 2 cut(s) 68, 1151
BspTI CTTAAG 2 cut(s) 2542, 3269
BspTNI GGTCTC 1 cut(s) 549
BsrDI GCAATG 3 cut(s) 452, 976, 1256
BsrFI RCCGGY 3 cut(s) 29, 42, 2537
BsrI ACTGG 8 cut(s) 340, 346, 403, 1611, 1902, 2532, 2874, 3043
BssAI RCCGGY 3 cut(s) 29, 42, 2537
BssECI CCNNGG 3 cut(s) 165, 1432, 1433
BssNI GRCGYC 2 cut(s) 27, 69
BssT1I CCWWGG 1 cut(s) 165
Bst6I CTCTTC 2 cut(s) 114, 2178
BstACI GRCGYC 2 cut(s) 27, 69
BstAFI CTTAAG 2 cut(s) 2542, 3269
BstBI TTCGAA 1 cut(s) 194
BstC8I GCNNGC 9 cut(s) 31, 201, 354, 488, 1612, 1923, 2539, 2796, 3301
BstDSI CCRYGG 1 cut(s) 165
BstEII GGTNACC 2 cut(s) 734, 1715
BstF5I GGATG 8 cut(s) 264, 299, 529, 530, 775, 1754, 1894, 1906
BstH2I RGCGCY 2 cut(s) 72, 2293
BstHHI GCGC 2 cut(s) 71, 2292
BstMAI GTCTC 5 cut(s) 462, 549, 1666, 1814, 3226
BstMWI GCNNNNNNNGC 8 cut(s) 136, 524, 625, 1018, 1457, 1497, 2084, 2126
BstNSI RCATGY 3 cut(s) 1414, 1474, 1528
BstPI GGTNACC 2 cut(s) 734, 1715
BstSCI CCNGG 3 cut(s) 493, 1431, 1432
BstSFI CTRYAG 1 cut(s) 3153
BstV2I GAAGAC 2 cut(s) 286, 2678
BstX2I RGATCY 6 cut(s) 172, 683, 1959, 2231, 2762, 3243
BstXI CCANNNNNNTGG 2 cut(s) 674, 1126
BstYI RGATCY 6 cut(s) 172, 683, 1959, 2231, 2762, 3243
Bsu15I ATCGAT 2 cut(s) 2092, 2470
BsuI GTATCC 1 cut(s) 60
BsuRI GGCC 5 cut(s) 209, 345, 923, 1993, 2634
BsuTUI ATCGAT 2 cut(s) 2092, 2470
BtgI CCRYGG 1 cut(s) 165
BtrI CACGTC 2 cut(s) 9, 2693
BtsCI GGATG 8 cut(s) 264, 299, 529, 530, 775, 1754, 1894, 1906
BtsI GCAGTG 3 cut(s) 707, 884, 1155
BveI ACCTGC 1 cut(s) 764
Cac8I GCNNGC 9 cut(s) 31, 201, 354, 488, 1612, 1923, 2539, 2796, 3301
CaiI CAGNNNCTG 1 cut(s) 2408
CciI TCATGA 1 cut(s) 2568
CfoI GCGC 2 cut(s) 71, 2292
Cfr10I RCCGGY 3 cut(s) 29, 42, 2537
Cfr13I GGNCC 3 cut(s) 208, 1991, 3121
Cfr9I CCCGGG 1 cut(s) 1432
ClaI ATCGAT 2 cut(s) 2092, 2470
CseI GACGC 2 cut(s) 35, 306
Csp6I GTAC 4 cut(s) 572, 1468, 1895, 2739
CspAI ACCGGT 1 cut(s) 42
CspCI CAANNNNNGTGG 6 cut(s) 490, 525, 2010, 2045, 2131, 2166
CviQI GTAC 4 cut(s) 572, 1468, 1895, 2739
DinI GGCGCC 1 cut(s) 70
DraIII CACNNNGTG 1 cut(s) 1979
EaeI YGGCCR 2 cut(s) 343, 921
Eam1104I CTCTTC 2 cut(s) 114, 2178
EarI CTCTTC 2 cut(s) 114, 2178
EciI GGCGGA 1 cut(s) 185
Ecl136II GAGCTC 1 cut(s) 2078
Eco130I CCWWGG 1 cut(s) 165
Eco147I AGGCCT 1 cut(s) 2634
Eco24I GRGCYC 1 cut(s) 2080
Eco31I GGTCTC 1 cut(s) 549
Eco32I GATATC 1 cut(s) 1346
Eco47I GGWCC 1 cut(s) 3121
Eco53kI GAGCTC 1 cut(s) 2078
Eco57I CTGAAG 1 cut(s) 3147
Eco88I CYCGRG 1 cut(s) 1432
Eco91I GGTNACC 2 cut(s) 734, 1715
EcoICRI GAGCTC 1 cut(s) 2078
EcoO65I GGTNACC 2 cut(s) 734, 1715
EcoRI GAATTC 1 cut(s) 3130
EcoRV GATATC 1 cut(s) 1346
EcoT14I CCWWGG 1 cut(s) 165
EcoT22I ATGCAT 2 cut(s) 1756, 1784
EcoT38I GRGCYC 1 cut(s) 2080
EgeI GGCGCC 1 cut(s) 70
EheI GGCGCC 1 cut(s) 70
ErhI CCWWGG 1 cut(s) 165
Esp3I CGTCTC 1 cut(s) 462
FalI AAGNNNNNCTT 4 cut(s) 1848, 1880, 2853, 2885
FaqI GGGAC 3 cut(s) 2147, 2245, 2703
FbaI TGATCA 1 cut(s) 478
FblI GTMKAC 3 cut(s) 5, 727, 1295
FokI GGATG 8 cut(s) 271, 286, 517, 536, 782, 1741, 1901, 1913
FriOI GRGCYC 1 cut(s) 2080
GlaI GCGC 2 cut(s) 70, 2291
GsaI CCCAGC 1 cut(s) 1992
GsuI CTGGAG 4 cut(s) 696, 1560, 1813, 2501
HaeII RGCGCY 2 cut(s) 72, 2293
HaeIII GGCC 5 cut(s) 209, 345, 923, 1993, 2634
HapII CCGG 8 cut(s) 30, 43, 428, 494, 641, 1433, 2050, 2538
HgaI GACGC 2 cut(s) 35, 306
HhaI GCGC 2 cut(s) 71, 2292
Hin1I GRCGYC 2 cut(s) 27, 69
Hin6I GCGC 2 cut(s) 69, 2290
HinP1I GCGC 2 cut(s) 69, 2290
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HindIII AAGCTT 3 cut(s) 197, 488, 1512
HpaII CCGG 8 cut(s) 30, 43, 428, 494, 641, 1433, 2050, 2538
HphI GGTGA 7 cut(s) 746, 788, 1709, 1992, 2587, 2924, 3021
Hpy166II GTNNAC 5 cut(s) 6, 728, 1296, 1522, 3029
Hpy8I GTNNAC 5 cut(s) 6, 728, 1296, 1522, 3029
Hpy99I CGWCG 6 cut(s) 10, 29, 290, 322, 325, 922
HpyCH4IV ACGT 3 cut(s) 8, 715, 2692
HpyF10VI GCNNNNNNNGC 8 cut(s) 136, 524, 625, 1018, 1457, 1497, 2084, 2126
HpySE526I ACGT 3 cut(s) 8, 715, 2692
Hsp92I GRCGYC 2 cut(s) 27, 69
HspAI GCGC 2 cut(s) 69, 2290
KasI GGCGCC 1 cut(s) 68
Kpn2I TCCGGA 2 cut(s) 427, 640
KroI GCCGGC 2 cut(s) 29, 2537
KroNI GCCGGC 2 cut(s) 31, 2539
Ksp22I TGATCA 1 cut(s) 478
LmnI GCTCC 8 cut(s) 1505, 1541, 1832, 1967, 2125, 2375, 2972, 3061
MaeII ACGT 3 cut(s) 8, 715, 2692
MfeI CAATTG 1 cut(s) 3051
MflI RGATCY 6 cut(s) 172, 683, 1959, 2231, 2762, 3243
MhlI GDGCHC 2 cut(s) 2080, 2122
MlsI TGGCCA 1 cut(s) 345
MluNI TGGCCA 1 cut(s) 345
Mly113I GGCGCC 1 cut(s) 69
MlyI GAGTC 5 cut(s) 208, 733, 773, 2190, 2267
MmeI TCCRAC 7 cut(s) 525, 1084, 1266, 1332, 1924, 2235, 2566
Mox20I TGGCCA 1 cut(s) 345
Mph1103I ATGCAT 2 cut(s) 1756, 1784
MroI TCCGGA 2 cut(s) 427, 640
MroNI GCCGGC 2 cut(s) 29, 2537
MroXI GAANNNNTTC 2 cut(s) 1207, 1333
MscI TGGCCA 1 cut(s) 345
MslI CAYNNNNRTG 5 cut(s) 1160, 1266, 2061, 2350, 2573
Msp20I TGGCCA 1 cut(s) 345
MspA1I CMGCKG 1 cut(s) 674
MspCI CTTAAG 2 cut(s) 2542, 3269
MspI CCGG 8 cut(s) 30, 43, 428, 494, 641, 1433, 2050, 2538
MspR9I CCNGG 3 cut(s) 495, 1433, 1434
MunI CAATTG 1 cut(s) 3051
Mva1269I GAATGC 2 cut(s) 1395, 2894
MwoI GCNNNNNNNGC 8 cut(s) 136, 524, 625, 1018, 1457, 1497, 2084, 2126
NaeI GCCGGC 2 cut(s) 31, 2539
NarI GGCGCC 1 cut(s) 69
NciI CCSGG 3 cut(s) 495, 1433, 1434
NcoI CCATGG 1 cut(s) 165
NgoMIV GCCGGC 2 cut(s) 29, 2537
NheI GCTAGC 1 cut(s) 3299
NlaIV GGNNCC 6 cut(s) 70, 499, 1153, 2651, 2764, 2968
NmuCI GTSAC 9 cut(s) 734, 776, 1110, 1573, 1715, 1805, 2182, 2557, 2956
NsiI ATGCAT 2 cut(s) 1756, 1784
NspI RCATGY 3 cut(s) 1414, 1474, 1528
NspV TTCGAA 1 cut(s) 194
OliI CACNNNNGTG 1 cut(s) 2350
PacI TTAATTAA 1 cut(s) 555
PagI TCATGA 1 cut(s) 2568
PaqCI CACCTGC 1 cut(s) 764
PceI AGGCCT 1 cut(s) 2634
PciI ACATGT 3 cut(s) 1410, 1470, 1524
PcsI WCGNNNNNNNCGW 1 cut(s) 2698
PctI GAATGC 2 cut(s) 1395, 2894
PdiI GCCGGC 2 cut(s) 31, 2539
PdmI GAANNNNTTC 2 cut(s) 1207, 1333
PfeI GAWTC 8 cut(s) 220, 1484, 1708, 1738, 2392, 2993, 3002, 3047
PflMI CCANNNNNTGG 3 cut(s) 1528, 1569, 3038
PinAI ACCGGT 1 cut(s) 42
PleI GAGTC 5 cut(s) 208, 732, 773, 2189, 2266
PluTI GGCGCC 1 cut(s) 72
PpsI GAGTC 5 cut(s) 208, 732, 773, 2189, 2266
PscI ACATGT 3 cut(s) 1410, 1470, 1524
PshBI ATTAAT 2 cut(s) 555, 1095
Psp124BI GAGCTC 1 cut(s) 2080
PspEI GGTNACC 2 cut(s) 734, 1715
PspFI CCCAGC 1 cut(s) 1988
PspN4I GGNNCC 6 cut(s) 70, 499, 1153, 2651, 2764, 2968
PspPI GGNCC 3 cut(s) 208, 1991, 3121
PstNI CAGNNNCTG 1 cut(s) 2408
PsuI RGATCY 6 cut(s) 172, 683, 1959, 2231, 2762, 3243
PvuII CAGCTG 1 cut(s) 674
RsaI GTAC 4 cut(s) 573, 1469, 1896, 2740
RsaNI GTAC 4 cut(s) 572, 1468, 1895, 2739
RseI CAYNNNNRTG 5 cut(s) 1160, 1266, 2061, 2350, 2573
SacI GAGCTC 1 cut(s) 2080
SalI GTCGAC 1 cut(s) 4
Sau96I GGNCC 3 cut(s) 208, 1991, 3121
SchI GAGTC 5 cut(s) 208, 733, 773, 2190, 2267
ScrFI CCNGG 3 cut(s) 495, 1433, 1434
SduI GDGCHC 2 cut(s) 2080, 2122
SfcI CTRYAG 1 cut(s) 3153
SfoI GGCGCC 1 cut(s) 70
SfuI TTCGAA 1 cut(s) 194
SinI GGWCC 1 cut(s) 3121
SmaI CCCGGG 1 cut(s) 1434
SmiMI CAYNNNNRTG 5 cut(s) 1160, 1266, 2061, 2350, 2573
SmlI CTYRAG 5 cut(s) 140, 1673, 2006, 2542, 3269
SmoI CTYRAG 5 cut(s) 140, 1673, 2006, 2542, 3269
SseBI AGGCCT 1 cut(s) 2634
SsiI CCGC 2 cut(s) 170, 910
SspDI GGCGCC 1 cut(s) 68
SspI AATATT 1 cut(s) 1382
SstI GAGCTC 1 cut(s) 2080
StuI AGGCCT 1 cut(s) 2634
StyD4I CCNGG 3 cut(s) 493, 1431, 1432
StyI CCWWGG 1 cut(s) 165
TaiI ACGT 3 cut(s) 11, 718, 2695
TaqI TCGA 7 cut(s) 5, 146, 194, 1913, 2092, 2470, 2802
TatI WGTACW 3 cut(s) 571, 1467, 1894
TfiI GAWTC 8 cut(s) 220, 1484, 1708, 1738, 2392, 2993, 3002, 3047
TseFI GTSAC 9 cut(s) 734, 776, 1110, 1573, 1715, 1805, 2182, 2557, 2956
Tsp45I GTSAC 9 cut(s) 734, 776, 1110, 1573, 1715, 1805, 2182, 2557, 2956
TspDTI ATGAA 9 cut(s) 101, 642, 831, 987, 1178, 1497, 1710, 2557, 3143
TspGWI ACGGA 5 cut(s) 285, 537, 772, 1271, 2203
TspMI CCCGGG 1 cut(s) 1432
Van91I CCANNNNNTGG 3 cut(s) 1528, 1569, 3038
Vha464I CTTAAG 2 cut(s) 2542, 3269
VpaK11BI GGWCC 1 cut(s) 3121
VspI ATTAAT 2 cut(s) 555, 1095
XapI RAATTY 7 cut(s) 607, 928, 1648, 2418, 2879, 2983, 3130
XbaI TCTAGA 1 cut(s) 2395
XceI RCATGY 3 cut(s) 1414, 1474, 1528
XcmI CCANNNNNNNNNTGG 1 cut(s) 401
XmaI CCCGGG 1 cut(s) 1432
XmiI GTMKAC 3 cut(s) 5, 727, 1295
XmnI GAANNNNTTC 2 cut(s) 1207, 1333
Zsp2I ATGCAT 2 cut(s) 1756, 1784
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.