FvH4_1g25820

BRCA2, oligonucleotide/oligosaccharide-binding, domain 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0004075
Physical Location & Seq
Reverse (-)
17729995 .. 17738105
8111 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g25820.t5

Sequence Viewer

Length: 3654 bp
ATGTCGACGTGGCACATGTTCTCCGACGCCGCCGACAATTTCCGGTGGCGATTCTCCGCTCCGGACCCCAACACCAGGCCGCCGGACGCCGCCTTCTATACCGGCAACGAAAATCTCAGCTCAAGCGCTCGCTTGCCTTCCATGGCCGACCTGTTGCTTCAAGGCTGCTCAAAGCTCGCGGCGGAGAGTCAGATGAGTCACGGCGGACTCGGCAGATCCGTCTCCGTCAAGCCGTCTTCCTTAGCCAAAGCAGCTTCGCTTCTGGGGGCCTCCGATTCAGGCCGAATGAGAGGTAGTGGAGGAGAGATTAGAGGTGATGGTTTCGGTGATTCGCTTTTCCAGACTGCCTCTGGGAAGATGGTTAATATATCTTCCAAAGGTCTTGTCAAGGCCAAGAAATTGCTTAGTTTGGAAGATGAGAATGAGGACTGTAGTCTTTTTTCGGGGTTCAACATTGGTGGGGTTAAGGATGCTGCATCGGTTTCAAGGTCTTCGGTGAATCATATGACTGGTTCGGTGCAAAGTGGGTTGACGGATGTGTCCAATCTGAGTTCTGTGCAAGCTCAAGTGCCTAGTTTGGCTTGTACACCTTCATCAGTGAAGCTACATACACCCGGAGGAAGATCTACTTCTGTTTCTACTGATGGATGTATGGCTGGTTCCATGCAGAGTAGATGTAAGAATGAGTTTGATCAGAGATTTATGCAATCCGAGATGACTAATTCAGTTTCCACACCTTCATCAGTCCAGTTACCTACACCCAGTTCAATTGATGCACACAAGACTGGTTTGGTGCAAAGTAGATTTAAGAATGAGTCCAAGCTAAATCATTTGCAATCCAAAATGCCTAGCTCAGCTTCTACTCCTTCATCATCCAAGCTACTTGCAGCAGGAGGAAGCTCCGAATGTCTTTCCACTGATCCACATAAGACTGGTTCAGTGCAAAGTGGAGTTACAAATGTGACTGATCTGAGTGTTATGCGATTCCAGAAGCCTAATATAGCACCCAAACCTTCATCAATCAAATTCAGTACAGCTGGAGGAAGATCTATATCTGTATCCACTGATGCACTACAACGTGCTAGGAATCTCCTTGGTGATCCAGACTTGGGAACTCTCCTGGATGAGGGGAATTCAGACATGGATCTGACATTTTCAAAAGGTTGTGAACATGATAGTCACACTGCCTCTTCCCACCAGAAAAAGCCAAAGTCCAAATTTTTAACAAAGAGTTTTGTATCTCCACTGCAATCATCGACTAATCAAGTGCAGACATCAGCAGTCAAGTCAGATAGTACAAATTGGGGCACTAACCTGATCAGCCAATTCGATGTTGTTAGCAATGAAAATGCCCGCAGGTCAAATGGTGAGTTACCTTGTGGCAAAAAACCCTTCAGCAACAAACCTTGTACTCTCACTACAGCAGAGAACAATTATTTGGCAAATGGTAATGGTGAAAGGATTGATCCTGTTGAAAGGTCACTAGCCAAGCCATTGGTGGATATTTCAAATACCATTGGCACCTCTCCTCTGAGTAATATACGAATGGCTGGTGTAAAGAGGAAGCTAGGAGGGAATTCTGTTTCTTCATTCAAGAAGCCTCGCATTTCTAACTTCTCCACCCCATTGCATAGGAATGTTCCACTCGTTCCTAATGGTTTGTCTACTTTTCCCTCTGACCATTCAAGTTTCAAAGGAAGGGTTTCTACTCGATATCCTGTCCTGGTGGCTAGAATGCCTTTGAAAAAGTATTTTGGAATGCCACCATCAGATCAAACCATGGTGGACTGCATGTCAGATCAGGTGAGAAGAGTTACAGCAATTAATGCAGAAAACTACATGTTTCCTGATGAATCTGGCTTAAACTACGTAGGAGCAGAAGCTGTTATGCACATTTTGGCTTCTTCGGGAGCCTCAACAAAAAATACTTTCAGAGAGTGGGTCATAAATCACTACAAGTGGATTGTCTGGAAACTGTCTTGTTATGAGAGATGCTATCCCACTAAAGCTTTAGGAAAATTTTTGACGCTGCCCAATGTGCTTGAAGAACTGAAGTATAGATATGAAAGAGAAGTGAATCATGGTCACCGCTCTGCAATTAAGAAAATACTGGAAGGAGATGCGTCACCTTCTTCAATGTTGGTCTTATGCATTTCAGCTATCCGCACAAAACATGGCCCAAATATAGAGACTAGCTCAGGAGCTGAAAATAGTAGTGCTGCAAAAGTTGAACTTACTGATGGGTGGTATTCAGTGGATGCTATTCTGGATGCTCTCCTATCAAAGCAGCTCGCTAGTGGAAAATTGTTTGTCGGACTGAAACTTCGGATTTGGGGAGCAGGACTCTGCGGCTGGGTTGGGCCTATTTCACCCCTCGAGGTGTCAAGAGAAGTTATGTTACGGCTACACATAAATGGCACATATAGAGCTCACTGGGATGATCGATTGGGATTTTGCAACCGTGTTGGCCCTCCCTTAGCATTTAAGTGCATCAAGAGCGATGGGGGTCTAATTCCTTGGACTCTTGTAGGAGTCACACGGATTTACCCTGTTTTATACAAAGAAAGGTTAAGTAATGGAAGATCTGTTGTGAGATCAGAGAGGTTGGAGACCAAAATGATGCAATCTTATAGCCAGAGGCGCTCTAATGTTATTGAGGGCATTATCTCTGAGTTCCAAAGAGGACTAGAACATTCACATACATGTAATGAGAGAGACAGTGAAGGAGCAAAACTTTTGAGGATACTAGAGACAGCTGCTGAACCTGAAGTTTTGATGGCGGAGATGAGTACAGAACAATTGAATTCTTTTACCAAATATCGAGCAAAGTTAGAGGCAATCAAGCAGTCAGACATGGAGAAATCAATTACGAAAGCACTAGAAGATGCTGGATTAGCTGAGAGGGAAGTGATGCCATTAATGAGGGTGAGGGTAGTTGGATTAACTAGAAGCTTATGTAAAGGAAAAGACAGTCCTAAAGAAGGACTAATAACAATCTGGAACCCGTCAGAGCAGCAGAAGTCTGAGTTGGTTGAGGGGCAAGCATATACTGTTTCTGACCTTATACCAACAAGTCCGGCTGCTGATATTCTATACTTGCTAGGAAGAGGATCCAAAACAAAGTGGAAGCCCTTATGTCAGCAGACAATGGACAACTTCAGGCCCTTTTTCAGTCCTCGCAAATCAATGTTATTGTCAGATTTTGGTCAAGTTCCTCTTTCCAGTGAATTTGATATTGCTGCATTCGTGCTGTTTGTGGGGGATGTTTATATAGCGGGTCACCAGAAGAAACAGTGGGTGTTTGTGACAGATGGTTCCATTTCTGAATCAAAATCAGAAGGACTAAACGATTCTTTACTTGCCATCTGCTTCTGTTCACCGCATGTTGATGATCAGTCAGATCCTCCTTTAAACTACAACCTCGCAGGTTCAACGGTTGGTTTTTGTAATCTCATCAAGAGAGCAAAGGACCAAATGAATGCTCTTTGGGTAGCTGAAGCTACAGAAAACTCGGCATACTTTTTGAGTTTTGACTCTCGACATTGTTCACATCTCAAAGATGCTGCTGCCTCTGCTGAAAGATGGGGCAGGATCTCTAGCTTGATGATCAATAGACTCAAGGAGAGGGTTCAATTTATTATTGGTAAAGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000226 GO:0000228 GO:0000278 GO:0000280 GO:0000281 GO:0000722 GO:0000723 GO:0000724 GO:0000725 GO:0000781 GO:0000784 GO:0000793 GO:0000794 GO:0000795 GO:0000800 GO:0000910 GO:0001556 GO:0001701 GO:0001824 GO:0001832 GO:0001833 GO:0002020 GO:0002376 GO:0002520 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003697 GO:0003824 GO:0004402 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005739 GO:0005813 GO:0005815 GO:0005829 GO:0005856 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006275 GO:0006281 GO:0006289 GO:0006302 GO:0006310 GO:0006312 GO:0006325 GO:0006355 GO:0006464 GO:0006473 GO:0006475 GO:0006725 GO:0006807 GO:0006915 GO:0006950 GO:0006974 GO:0006978 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007059 GO:0007098 GO:0007127 GO:0007140 GO:0007141 GO:0007154 GO:0007165 GO:0007275 GO:0007276 GO:0007281 GO:0007283 GO:0007292 GO:0007346 GO:0007399 GO:0007417 GO:0007420 GO:0007548 GO:0007568 GO:0007569 GO:0008022 GO:0008080 GO:0008092 GO:0008104 GO:0008150 GO:0008152 GO:0008156 GO:0008219 GO:0008283 GO:0008285 GO:0008406 GO:0008585 GO:0008608 GO:0008630 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009790 GO:0009792 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0009994 GO:0010165 GO:0010212 GO:0010225 GO:0010332 GO:0010468 GO:0010484 GO:0010485 GO:0010556 GO:0010557 GO:0010558 GO:0010564 GO:0010604 GO:0010605 GO:0010628 GO:0012501 GO:0012505 GO:0015630 GO:0015631 GO:0016043 GO:0016407 GO:0016410 GO:0016569 GO:0016570 GO:0016573 GO:0016740 GO:0016746 GO:0016747 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019538 GO:0019899 GO:0019953 GO:0021700 GO:0022402 GO:0022412 GO:0022414 GO:0023052 GO:0030097 GO:0030141 GO:0030154 GO:0030330 GO:0030879 GO:0031023 GO:0031297 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031410 GO:0031619 GO:0031974 GO:0031981 GO:0031982 GO:0032200 GO:0032465 GO:0032501 GO:0032502 GO:0032504 GO:0032991 GO:0033036 GO:0033260 GO:0033365 GO:0033554 GO:0033593 GO:0033599 GO:0033600 GO:0034212 GO:0034502 GO:0034613 GO:0034641 GO:0034645 GO:0035264 GO:0035556 GO:0036211 GO:0040007 GO:0042127 GO:0042592 GO:0042770 GO:0042771 GO:0042772 GO:0042802 GO:0043009 GO:0043015 GO:0043060 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043543 GO:0043966 GO:0043967 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044454 GO:0044464 GO:0044703 GO:0044786 GO:0045005 GO:0045132 GO:0045137 GO:0045143 GO:0045184 GO:0045787 GO:0045893 GO:0045931 GO:0045934 GO:0045935 GO:0046483 GO:0046545 GO:0046660 GO:0048232 GO:0048285 GO:0048468 GO:0048469 GO:0048477 GO:0048478 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048534 GO:0048589 GO:0048599 GO:0048608 GO:0048609 GO:0048731 GO:0048732 GO:0048856 GO:0048869 GO:0050000 GO:0050678 GO:0050680 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051052 GO:0051053 GO:0051093 GO:0051171 GO:0051172 GO:0051173 GO:0051179 GO:0051234 GO:0051239 GO:0051241 GO:0051252 GO:0051254 GO:0051276 GO:0051298 GO:0051301 GO:0051302 GO:0051303 GO:0051310 GO:0051311 GO:0051316 GO:0051321 GO:0051455 GO:0051640 GO:0051641 GO:0051649 GO:0051656 GO:0051704 GO:0051716 GO:0051726 GO:0060249 GO:0060255 GO:0060322 GO:0061458 GO:0061640 GO:0061733 GO:0061982 GO:0065007 GO:0065008 GO:0070013 GO:0070198 GO:0070199 GO:0070200 GO:0070727 GO:0071695 GO:0071704 GO:0071840 GO:0072331 GO:0072332 GO:0072594 GO:0080090 GO:0090304 GO:0090329 GO:0097159 GO:0097190 GO:0097193 GO:0097708 GO:0098687 GO:0098813 GO:0099086 GO:0099503 GO:0140013 GO:1901360 GO:1901363 GO:1901564 GO:1901576 GO:1902298 GO:1902680 GO:1902969 GO:1903046 GO:1903047 GO:1903506 GO:1903508 GO:1990426 GO:1990505 GO:2000026 GO:2000104 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03440 ko03460 ko05200 ko05212 ko05224 map03440 map03460 map05200 map05212 map05224
Pfam Domains
Protein Families
BRCA-2_OB1 BRCA-2_helical BRCA2

Protein Analysis

1218

Amino Acids

132.76

Weight (kDa)

9.07

Isoelectric Point (pI)

44.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BRCA2 PF00634 111 - 136 2.2e-07 BRCA2 repeat
BRCA2 PF00634 342 - 367 3.6e-08 BRCA2 repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 538
AbsI CCTCGAGG 1 cut(s) 2375
Acc36I ACCTGC 2 cut(s) 1347, 3416
AccB1I GGYRCC 1 cut(s) 1520
AccBSI CCGCTC 2 cut(s) 59, 2091
AccI GTMKAC 2 cut(s) 5, 1664
AccII CGCG 1 cut(s) 179
AccIII TCCGGA 1 cut(s) 61
AclWI GGATC 9 cut(s) 210, 914, 1094, 1152, 1460, 3105, 3118, 3395, 3599
AcoI YGGCCR 1 cut(s) 144
AcsI RAATTY 7 cut(s) 1025, 1132, 1217, 1576, 2018, 2803, 3227
AcuI CTGAAG 5 cut(s) 1378, 2072, 2787, 3142, 3516
AcyI GRCGYC 2 cut(s) 27, 87
AfaI GTAC 5 cut(s) 586, 1033, 1297, 1411, 2791
AfeI AGCGCT 1 cut(s) 127
AfiI CCNNNNNNNGG 4 cut(s) 75, 1109, 1126, 2981
AflIII ACRYGT 3 cut(s) 15, 1839, 2702
AhdI GACNNNNNGTC 1 cut(s) 1792
AjiI CACGTC 1 cut(s) 9
AjnI CCWGG 3 cut(s) 74, 1119, 1722
AjuI GAANNNNNNNTTGG 4 cut(s) 1421, 1453, 3011, 3043
Alw21I GWGCWC 1 cut(s) 2431
Alw26I GTCTC 5 cut(s) 226, 2183, 2603, 2709, 2744
AlwI GGATC 9 cut(s) 210, 914, 1094, 1152, 1460, 3105, 3118, 3395, 3599
AlwNI CAGNNNCTG 3 cut(s) 1883, 2204, 2759
Ama87I CYCGRG 1 cut(s) 2375
Aor13HI TCCGGA 1 cut(s) 61
Aor51HI AGCGCT 1 cut(s) 127
AoxI GGCC 9 cut(s) 77, 144, 267, 280, 390, 2176, 2360, 2467, 3161
ApoI RAATTY 7 cut(s) 1025, 1132, 1217, 1576, 2018, 2803, 3227
ArsI GACNNNNNNTTYG 4 cut(s) 369, 401, 2307, 2339
AseI ATTAAT 2 cut(s) 1824, 2918
Asp700I GAANNNNTTC 1 cut(s) 1702
AspLEI GCGC 2 cut(s) 128, 2643
AspS9I GGNCC 7 cut(s) 64, 267, 2177, 2360, 2468, 3162, 3469
AsuC2I CCSGG 1 cut(s) 615
AvaI CYCGRG 1 cut(s) 2375
AvaII GGWCC 2 cut(s) 64, 3469
BaeGI GKGCMC 1 cut(s) 1310
BamHI GGATCC 1 cut(s) 3110
BanI GGYRCC 1 cut(s) 1520
BanII GRGCYC 1 cut(s) 2431
BarI GAAGNNNNNNTAC 4 cut(s) 1690, 1722, 2382, 2414
BbsI GAAGAC 2 cut(s) 228, 483
Bbv12I GWGCWC 1 cut(s) 2431
BceAI ACGGC 3 cut(s) 217, 217, 2417
BciT130I CCWGG 3 cut(s) 76, 1121, 1724
BciVI GTATCC 2 cut(s) 1069, 2736
BclI TGATCA 4 cut(s) 691, 1317, 3391, 3606
BcnI CCSGG 1 cut(s) 615
BcoDI GTCTC 5 cut(s) 226, 2183, 2603, 2709, 2744
BfmI CTRYAG 3 cut(s) 430, 1419, 3501
BfoI RGCGCY 2 cut(s) 129, 2644
BfuAI ACCTGC 2 cut(s) 1347, 3416
BfuI GTATCC 2 cut(s) 1069, 2736
BglII AGATCT 3 cut(s) 623, 1046, 2582
BlpI GCTNAGC 1 cut(s) 853
Bme1390I CCNGG 4 cut(s) 76, 615, 1121, 1724
Bme18I GGWCC 2 cut(s) 64, 3469
BmeRI GACNNNNNGTC 1 cut(s) 1792
BmeT110I CYCGRG 1 cut(s) 2375
BmgBI CACGTC 1 cut(s) 9
BmgT120I GGNCC 7 cut(s) 64, 267, 2177, 2360, 2468, 3162, 3469
BmiI GGNNCC 8 cut(s) 66, 268, 661, 1522, 1912, 3002, 3112, 3316
BmrFI CCNGG 4 cut(s) 76, 615, 1121, 1724
BmrI ACTGGG 2 cut(s) 756, 2443
BmuI ACTGGG 2 cut(s) 756, 2443
BoxI GACNNNNGTC 1 cut(s) 432
BpiI GAAGAC 2 cut(s) 228, 483
BplI GAGNNNNNCTC 4 cut(s) 2180, 2212, 2328, 2360
BpmI CTGGAG 1 cut(s) 1059
Bpu10I CCTNAGC 3 cut(s) 241, 2197, 2476
Bpu1102I GCTNAGC 1 cut(s) 853
BpuEI CTTGAG 3 cut(s) 106, 549, 3602
BpuMI CCSGG 1 cut(s) 615
Bsa29I ATCGAT 1 cut(s) 2443
BsaAI YACGTR 1 cut(s) 1870
BsaBI GATNNNNATC 1 cut(s) 1051
BsaHI GRCGYC 2 cut(s) 27, 87
BsaI GGTCTC 1 cut(s) 2603
BsaJI CCNNGG 4 cut(s) 141, 1093, 1779, 2516
BsaWI WCCGGW 2 cut(s) 42, 61
BsaXI ACNNNNNCTCC 2 cut(s) 5, 35
Bsc4I CCNNNNNNNGG 4 cut(s) 75, 1109, 1126, 2981
Bse118I RCCGGY 1 cut(s) 101
Bse1I ACTGG 8 cut(s) 514, 748, 762, 790, 937, 2115, 2438, 3222
Bse3DI GCAATG 2 cut(s) 1348, 1625
Bse8I GATNNNNATC 1 cut(s) 1051
BseAI TCCGGA 1 cut(s) 61
BseBI CCWGG 3 cut(s) 76, 1121, 1724
BseCI ATCGAT 1 cut(s) 2443
BseDI CCNNGG 4 cut(s) 141, 1093, 1779, 2516
BseGI GGATG 9 cut(s) 475, 541, 653, 872, 1129, 2263, 2275, 2443, 3268
BseJI GATNNNNATC 1 cut(s) 1051
BseLI CCNNNNNNNGG 4 cut(s) 75, 1109, 1126, 2981
BseMI GCAATG 2 cut(s) 1348, 1625
BseMII CTCAG 9 cut(s) 130, 539, 867, 962, 1523, 2211, 2661, 2889, 3015
BseNI ACTGG 8 cut(s) 514, 748, 762, 790, 937, 2115, 2438, 3222
BseRI GAGGAG 2 cut(s) 315, 1518
BseSI GKGCMC 1 cut(s) 1310
BseYI CCCAGC 1 cut(s) 2352
BsgI GTGCAG 1 cut(s) 1289
Bsh1236I CGCG 1 cut(s) 179
BshFI GGCC 9 cut(s) 79, 146, 269, 282, 392, 2178, 2362, 2469, 3163
BshNI GGYRCC 1 cut(s) 1520
BshVI ATCGAT 1 cut(s) 2443
BsiHKAI GWGCWC 1 cut(s) 2431
BsiHKCI CYCGRG 1 cut(s) 2375
BsiSI CCGG 6 cut(s) 43, 62, 83, 102, 615, 3077
BslI CCNNNNNNNGG 4 cut(s) 75, 1109, 1126, 2981
BsmAI GTCTC 5 cut(s) 226, 2183, 2603, 2709, 2744
BsmBI CGTCTC 1 cut(s) 226
BsmI GAATGC 4 cut(s) 1740, 1764, 3242, 3484
BsnI GGCC 9 cut(s) 79, 146, 269, 282, 392, 2178, 2362, 2469, 3163
Bso31I GGTCTC 1 cut(s) 2603
BsoBI CYCGRG 1 cut(s) 2375
Bsp1286I GDGCHC 2 cut(s) 1310, 2431
Bsp13I TCCGGA 1 cut(s) 61
Bsp1407I TGTACA 1 cut(s) 584
Bsp1720I GCTNAGC 1 cut(s) 853
Bsp19I CCATGG 2 cut(s) 141, 1779
BspANI GGCC 9 cut(s) 79, 146, 269, 282, 392, 2178, 2362, 2469, 3163
BspCNI CTCAG 9 cut(s) 129, 540, 866, 963, 1524, 2210, 2662, 2890, 3016
BspDI ATCGAT 1 cut(s) 2443
BspEI TCCGGA 1 cut(s) 61
BspFNI CGCG 1 cut(s) 179
BspLI GGNNCC 8 cut(s) 66, 268, 661, 1522, 1912, 3002, 3112, 3316
BspMI ACCTGC 2 cut(s) 1347, 3416
BspPI GGATC 9 cut(s) 210, 914, 1094, 1152, 1460, 3105, 3118, 3395, 3599
BspT107I GGYRCC 1 cut(s) 1520
BspTNI GGTCTC 1 cut(s) 2603
BsrBI CCGCTC 2 cut(s) 59, 2091
BsrDI GCAATG 2 cut(s) 1348, 1625
BsrFI RCCGGY 1 cut(s) 101
BsrGI TGTACA 1 cut(s) 584
BsrI ACTGG 8 cut(s) 514, 748, 762, 790, 937, 2115, 2438, 3222
BssAI RCCGGY 1 cut(s) 101
BssECI CCNNGG 4 cut(s) 141, 1093, 1779, 2516
BssNI GRCGYC 2 cut(s) 27, 87
BssT1I CCWWGG 4 cut(s) 141, 1093, 1779, 2516
Bst2UI CCWGG 3 cut(s) 76, 1121, 1724
Bst4CI ACNGT 8 cut(s) 431, 1977, 2462, 2720, 2972, 3052, 3294, 3436
Bst6I CTCTTC 3 cut(s) 1195, 1804, 3100
BstACI GRCGYC 2 cut(s) 27, 87
BstAPI GCANNNNNTGC 1 cut(s) 1826
BstAUI TGTACA 1 cut(s) 584
BstBAI YACGTR 1 cut(s) 1870
BstC8I GCNNGC 7 cut(s) 130, 134, 177, 561, 1354, 2292, 3042
BstDSI CCRYGG 2 cut(s) 141, 1779
BstEII GGTNACC 2 cut(s) 2084, 3278
BstENI CCTNNNNNAGG 2 cut(s) 1124, 2979
BstF5I GGATG 9 cut(s) 475, 541, 653, 872, 1129, 2263, 2275, 2443, 3268
BstFNI CGCG 1 cut(s) 179
BstH2I RGCGCY 2 cut(s) 129, 2644
BstHHI GCGC 2 cut(s) 128, 2643
BstMAI GTCTC 5 cut(s) 226, 2183, 2603, 2709, 2744
BstMWI GCNNNNNNNGC 8 cut(s) 210, 251, 1826, 2038, 2496, 2640, 2894, 3572
BstNI CCWGG 3 cut(s) 76, 1121, 1724
BstNSI RCATGY 5 cut(s) 19, 1795, 1843, 2706, 3386
BstPAI GACNNNNGTC 1 cut(s) 432
BstPI GGTNACC 2 cut(s) 2084, 3278
BstSCI CCNGG 4 cut(s) 74, 613, 1119, 1722
BstSFI CTRYAG 3 cut(s) 430, 1419, 3501
BstSLI GKGCMC 1 cut(s) 1310
BstSNI TACGTA 1 cut(s) 1870
BstUI CGCG 1 cut(s) 179
BstV2I GAAGAC 2 cut(s) 228, 483
BstX2I RGATCY 8 cut(s) 215, 623, 1046, 1144, 2582, 3110, 3400, 3591
BstXI CCANNNNNNTGG 1 cut(s) 1495
BstYI RGATCY 8 cut(s) 215, 623, 1046, 1144, 2582, 3110, 3400, 3591
Bsu15I ATCGAT 1 cut(s) 2443
BsuI GTATCC 2 cut(s) 1069, 2736
BsuRI GGCC 9 cut(s) 79, 146, 269, 282, 392, 2178, 2362, 2469, 3163
BsuTUI ATCGAT 1 cut(s) 2443
BtgI CCRYGG 2 cut(s) 141, 1779
BtgZI GCGATG 1 cut(s) 2514
BtrI CACGTC 1 cut(s) 9
BtsCI GGATG 9 cut(s) 475, 541, 653, 872, 1129, 2263, 2275, 2443, 3268
BtsI GCAGTG 2 cut(s) 1182, 1244
BveI ACCTGC 2 cut(s) 1347, 3416
Cac8I GCNNGC 7 cut(s) 130, 134, 177, 561, 1354, 2292, 3042
CaiI CAGNNNCTG 3 cut(s) 1883, 2204, 2759
CfoI GCGC 2 cut(s) 128, 2643
Cfr10I RCCGGY 1 cut(s) 101
Cfr13I GGNCC 7 cut(s) 64, 267, 2177, 2360, 2468, 3162, 3469
ClaI ATCGAT 1 cut(s) 2443
CseI GACGC 4 cut(s) 35, 95, 2035, 2112
Csp6I GTAC 5 cut(s) 585, 1032, 1296, 1410, 2790
CspCI CAANNNNNGTGG 2 cut(s) 439, 474
CviQI GTAC 5 cut(s) 585, 1032, 1296, 1410, 2790
DraI TTTAAA 1 cut(s) 3411
DrdI GACNNNNNNGTC 1 cut(s) 538
DriI GACNNNNNGTC 1 cut(s) 1792
DseDI GACNNNNNNGTC 1 cut(s) 538
EaeI YGGCCR 1 cut(s) 144
Eam1104I CTCTTC 3 cut(s) 1195, 1804, 3100
Eam1105I GACNNNNNGTC 1 cut(s) 1792
EarI CTCTTC 3 cut(s) 1195, 1804, 3100
EciI GGCGGA 3 cut(s) 197, 219, 2795
Ecl136II GAGCTC 1 cut(s) 2429
Eco105I TACGTA 1 cut(s) 1870
Eco130I CCWWGG 4 cut(s) 141, 1093, 1779, 2516
Eco24I GRGCYC 1 cut(s) 2431
Eco31I GGTCTC 1 cut(s) 2603
Eco32I GATATC 1 cut(s) 1715
Eco47I GGWCC 2 cut(s) 64, 3469
Eco47III AGCGCT 1 cut(s) 127
Eco53kI GAGCTC 1 cut(s) 2429
Eco57I CTGAAG 5 cut(s) 1378, 2072, 2787, 3142, 3516
Eco88I CYCGRG 1 cut(s) 2375
Eco91I GGTNACC 2 cut(s) 2084, 3278
EcoICRI GAGCTC 1 cut(s) 2429
EcoNI CCTNNNNNAGG 2 cut(s) 1124, 2979
EcoO109I RGGNCCY 2 cut(s) 267, 3162
EcoO65I GGTNACC 2 cut(s) 2084, 3278
EcoRI GAATTC 3 cut(s) 1132, 1576, 2803
EcoRII CCWGG 3 cut(s) 74, 1119, 1722
EcoRV GATATC 1 cut(s) 1715
EcoT14I CCWWGG 4 cut(s) 141, 1093, 1779, 2516
EcoT22I ATGCAT 1 cut(s) 2153
EcoT38I GRGCYC 1 cut(s) 2431
ErhI CCWWGG 4 cut(s) 141, 1093, 1779, 2516
Esp3I CGTCTC 1 cut(s) 226
FalI AAGNNNNNCTT 6 cut(s) 613, 645, 2217, 2249, 3201, 3233
FauI CCCGC 2 cut(s) 1361, 3268
FauNDI CATATG 1 cut(s) 504
FbaI TGATCA 4 cut(s) 691, 1317, 3391, 3606
FblI GTMKAC 2 cut(s) 5, 1664
FokI GGATG 9 cut(s) 482, 548, 660, 859, 1136, 2270, 2282, 2450, 3275
FriOI GRGCYC 1 cut(s) 2431
GlaI GCGC 2 cut(s) 127, 2642
GsaI CCCAGC 1 cut(s) 2356
GsuI CTGGAG 1 cut(s) 1059
HaeII RGCGCY 2 cut(s) 129, 2644
HaeIII GGCC 9 cut(s) 79, 146, 269, 282, 392, 2178, 2362, 2469, 3163
HapII CCGG 6 cut(s) 43, 62, 83, 102, 615, 3077
HgaI GACGC 4 cut(s) 35, 95, 2035, 2112
HhaI GCGC 2 cut(s) 128, 2643
Hin1I GRCGYC 2 cut(s) 27, 87
Hin6I GCGC 2 cut(s) 126, 2641
HinP1I GCGC 2 cut(s) 126, 2641
HincII GTYRAC 2 cut(s) 6, 531
HindII GTYRAC 2 cut(s) 6, 531
HindIII AAGCTT 2 cut(s) 2007, 2950
HpaII CCGG 6 cut(s) 43, 62, 83, 102, 615, 3077
Hpy166II GTNNAC 8 cut(s) 6, 531, 587, 1169, 1665, 1786, 3377, 3548
Hpy8I GTNNAC 8 cut(s) 6, 531, 587, 1169, 1665, 1786, 3377, 3548
Hpy99I CGWCG 2 cut(s) 10, 29
HpyCH4III ACNGT 8 cut(s) 431, 1977, 2462, 2720, 2972, 3052, 3294, 3436
HpyCH4IV ACGT 3 cut(s) 8, 1078, 1869
HpyF10VI GCNNNNNNNGC 8 cut(s) 210, 251, 1826, 2038, 2496, 2640, 2894, 3572
HpySE526I ACGT 3 cut(s) 8, 1078, 1869
Hsp92I GRCGYC 2 cut(s) 27, 87
HspAI GCGC 2 cut(s) 126, 2641
Kpn2I TCCGGA 1 cut(s) 61
Ksp22I TGATCA 4 cut(s) 691, 1317, 3391, 3606
LmnI GCTCC 7 cut(s) 64, 905, 1874, 1910, 2201, 2336, 2726
MaeII ACGT 3 cut(s) 8, 1078, 1869
MbiI CCGCTC 2 cut(s) 59, 2091
MfeI CAATTG 2 cut(s) 768, 2798
MflI RGATCY 8 cut(s) 215, 623, 1046, 1144, 2582, 3110, 3400, 3591
MhlI GDGCHC 2 cut(s) 1310, 2431
MlyI GAGTC 9 cut(s) 196, 201, 205, 824, 2337, 2515, 2541, 3527, 3609
MmeI TCCRAC 4 cut(s) 48, 2293, 2586, 2917
Mph1103I ATGCAT 1 cut(s) 2153
MroI TCCGGA 1 cut(s) 61
MroXI GAANNNNTTC 1 cut(s) 1702
MslI CAYNNNNRTG 6 cut(s) 1635, 2412, 2436, 2485, 2701, 3387
MspA1I CMGCKG 2 cut(s) 1037, 2756
MspI CCGG 6 cut(s) 43, 62, 83, 102, 615, 3077
MspR9I CCNGG 4 cut(s) 76, 615, 1121, 1724
MunI CAATTG 2 cut(s) 768, 2798
Mva1269I GAATGC 4 cut(s) 1740, 1764, 3242, 3484
MvaI CCWGG 3 cut(s) 76, 1121, 1724
MvnI CGCG 1 cut(s) 179
MwoI GCNNNNNNNGC 8 cut(s) 210, 251, 1826, 2038, 2496, 2640, 2894, 3572
NciI CCSGG 1 cut(s) 615
NcoI CCATGG 2 cut(s) 141, 1779
NdeI CATATG 1 cut(s) 504
NlaIV GGNNCC 8 cut(s) 66, 268, 661, 1522, 1912, 3002, 3112, 3316
NmeAIII GCCGAG 2 cut(s) 189, 3491
NmuCI GTSAC 9 cut(s) 197, 961, 1178, 1479, 2084, 2124, 2533, 3278, 3304
NsiI ATGCAT 1 cut(s) 2153
NspI RCATGY 5 cut(s) 19, 1795, 1843, 2706, 3386
PaeR7I CTCGAG 1 cut(s) 2375
PciI ACATGT 3 cut(s) 15, 1839, 2702
PcsI WCGNNNNNNNCGW 1 cut(s) 216
PctI GAATGC 4 cut(s) 1740, 1764, 3242, 3484
PdmI GAANNNNTTC 1 cut(s) 1702
PfoI TCCNGGA 1 cut(s) 1119
PleI GAGTC 9 cut(s) 195, 201, 204, 823, 2337, 2515, 2540, 3527, 3609
PpsI GAGTC 9 cut(s) 195, 201, 204, 823, 2337, 2515, 2540, 3527, 3609
Ppu21I YACGTR 1 cut(s) 1870
PscI ACATGT 3 cut(s) 15, 1839, 2702
PshAI GACNNNNGTC 1 cut(s) 432
PshBI ATTAAT 2 cut(s) 1824, 2918
Psp124BI GAGCTC 1 cut(s) 2431
Psp6I CCWGG 3 cut(s) 74, 1119, 1722
PspEI GGTNACC 2 cut(s) 2084, 3278
PspFI CCCAGC 1 cut(s) 2352
PspGI CCWGG 3 cut(s) 74, 1119, 1722
PspN4I GGNNCC 8 cut(s) 66, 268, 661, 1522, 1912, 3002, 3112, 3316
PspPI GGNCC 7 cut(s) 64, 267, 2177, 2360, 2468, 3162, 3469
PspXI VCTCGAGB 1 cut(s) 2375
PsrI GAACNNNNNNTAC 2 cut(s) 643, 675
PstNI CAGNNNCTG 3 cut(s) 1883, 2204, 2759
PsuI RGATCY 8 cut(s) 215, 623, 1046, 1144, 2582, 3110, 3400, 3591
PvuII CAGCTG 2 cut(s) 1037, 2756
RsaI GTAC 5 cut(s) 586, 1033, 1297, 1411, 2791
RsaNI GTAC 5 cut(s) 585, 1032, 1296, 1410, 2790
RseI CAYNNNNRTG 6 cut(s) 1635, 2412, 2436, 2485, 2701, 3387
SacI GAGCTC 1 cut(s) 2431
SalI GTCGAC 1 cut(s) 4
Sau96I GGNCC 7 cut(s) 64, 267, 2177, 2360, 2468, 3162, 3469
SchI GAGTC 9 cut(s) 196, 201, 205, 824, 2337, 2515, 2541, 3527, 3609
ScrFI CCNGG 4 cut(s) 76, 615, 1121, 1724
SduI GDGCHC 2 cut(s) 1310, 2431
SfcI CTRYAG 3 cut(s) 430, 1419, 3501
Sfr274I CTCGAG 1 cut(s) 2375
SinI GGWCC 2 cut(s) 64, 3469
SlaI CTCGAG 1 cut(s) 2375
SmiMI CAYNNNNRTG 6 cut(s) 1635, 2412, 2436, 2485, 2701, 3387
SmlI CTYRAG 4 cut(s) 121, 564, 2375, 3617
SmoI CTYRAG 4 cut(s) 121, 564, 2375, 3617
SnaBI TACGTA 1 cut(s) 1870
SstI GAGCTC 1 cut(s) 2431
StyD4I CCNGG 4 cut(s) 74, 613, 1119, 1722
StyI CCWWGG 4 cut(s) 141, 1093, 1779, 2516
TaaI ACNGT 8 cut(s) 431, 1977, 2462, 2720, 2972, 3052, 3294, 3436
TaiI ACGT 3 cut(s) 11, 1081, 1872
TaqI TCGA 8 cut(s) 5, 1256, 1329, 1711, 2376, 2443, 2821, 3538
TatI WGTACW 5 cut(s) 584, 1031, 1295, 1409, 2789
TauI GCSGC 5 cut(s) 32, 82, 92, 182, 2352
TseFI GTSAC 9 cut(s) 197, 961, 1178, 1479, 2084, 2124, 2533, 3278, 3304
Tsp45I GTSAC 9 cut(s) 197, 961, 1178, 1479, 2084, 2124, 2533, 3278, 3304
TspDTI ATGAA 9 cut(s) 582, 729, 858, 1005, 1359, 1578, 1866, 2079, 3491
TspGWI ACGGA 4 cut(s) 208, 214, 548, 2554
VpaK11BI GGWCC 2 cut(s) 64, 3469
VspI ATTAAT 2 cut(s) 1824, 2918
XagI CCTNNNNNAGG 2 cut(s) 1124, 2979
XapI RAATTY 7 cut(s) 1025, 1132, 1217, 1576, 2018, 2803, 3227
XceI RCATGY 5 cut(s) 19, 1795, 1843, 2706, 3386
XcmI CCANNNNNNNNNTGG 2 cut(s) 347, 1495
XhoI CTCGAG 1 cut(s) 2375
XmiI GTMKAC 2 cut(s) 5, 1664
XmnI GAANNNNTTC 1 cut(s) 1702
Zsp2I ATGCAT 1 cut(s) 2153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.