Rroxscaffold_6G00397350

BRCA2, oligonucleotide/oligosaccharide-binding, domain 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0004075
Physical Location & Seq
Forward (+)
19036005 .. 19043034
7030 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00397350.1

Sequence Viewer

Length: 2964 bp
ATGTCCACATGGCACATGTTCTCCGACGCCGGCGGCGGCGATTTCCCGGTGGCGCATATCGGGTTCCGACCCCAATACCCCACCGCCGGACGCCGCCTTCTATTCGGACAACGAAAATCTCAGCTCAAGCTCAAGCTCAAGCACTCGCCTGCCTTCCATGGCCGACCTGTTGCTTCAAGGCTGTTCAAAACTCGCGGCGGAGAGTCAGAGAAATCACGACGCAGGTGTTGCCATGTTTCGCAATGGATTCGGAAGATCCGTTTCCGTCAAGCCGTCGTCGTTAGCCAAAGCGGCGTCGCTTCTGAGCTCCGAATCAGGTTAGGCCGAATGAAAGGTATAGAAAGTGAGAGTAGAGGTGGTTTCGGTGATTCCCTTTTCCAGACTGCCTCTGGGAAGATGGTTAATATATCTTCCAAAGGTCTTGTCAAGGCCAAGAGATTGCTTGGTTTGGAAGATGATAATGGTGACTGTAATCTTACCGGTTTCAGCACTACTGGGGTTAAGGATGCTGTATCGGTTTCAAGGTCTTTGTTGAATCATACGACTGGTTCGGCGCAAACTGGGTTAACGAATGTGTCCAATCTAAATTCTGTGCAAGCTGAAGTGCCTAGTACAGCTCGTACACCTTCATCAGTCAAGTTACATACACCCGGAGGGGGATCTACTTCTGTTTCTAGTGATGCATTTAAGACAGGTTCTGTGCAGAGTAGATTGCAAAGTAGATTTAAGAATGAGTCAAAACTAAATTATATGCAATCCGAGCCTAGTTCAGCTTGTACGCCTTCATCAGCCAAGCTACTTGCACCTGGAGGAAGCTCTAAATCTCTTTCCACTGGTGCACATAAGACTGGTTCTCTGGAGGAAGATCTATCTGTTTCCACTGATGCACTACAACGTGCAAGGAGTCTTCTTGGTGACCCAGACTTGGGAACTCTCCTGAATGAGGGGAACTCAGACTTGGATCTGACATTTTCAAAAGGTCAAATGGTGAGTTACCTTGTGGGAAAAAAACCCTTCAGCAACAAACCCTGTACCCTTACTACAGTAGAAAACAATTATTTGGCAAATGGCACTGGTGAAAGGATTAATCCTGTTGAAAGGTCACTAGCCAAGCCATTGGTGGATATTTCAAATACCATTGGCACCACTCCTCTGAGTAATATACAAATGGCTGGTGTAAAGAGGAGGCTTGGAGGAAATTCTATTTCTCCATTCAAGAAGCCTCGCATTTCCAACTTCTCCACCCCATTGCATAAGAATGTTCCACTTGTTCCTAATGGTTTGTCTATTTTTCCGTCTGACCATTCAAGTGGCAAAAGAAGGGTTTGTACACGATATCCTGTCCTGGTGACCAGAATGTCTTTGAAGAAATATTTTGGAATGCCACCATCAGATCAAACCATGGTGGACTGCATGTCAGATCAGGTGAGAAGAGTTACAGCAATTAATGCAGAAAACTACATGTTTCCTGATGAATCTGGCTTAAACTACGTAGGGGCAGAGGCTTTTGTCCACATGTTGGCTTCTTCCGGAGCCTCAACACAAAATACTTCCAGAGAGTGGGTCATAAATCACTACAAGTGGATTGTCTGGAAACTGGCTTGTTATGAGAGATGCTATCCAGCTAAAGCTTTAGGAAAATTTTTGACACTGCCCAATGTGCTTGAAGAATTGAAGTATAGAGAAGTGAATCATGGTCACCGCTCTGCAATTAAGAAAATACTGGAAGGAGATGCATTACCTTCTTCCATGTTGGTCTTATGCATTTCAGCTATTCGCTCAAAACCTGACCCAAATATAGAGACTAGCTCCGGAGCTGAAAATAGTTGTGCCACAAAAGTTGAACTTACTGATGGGTGGTATTCAGTGGATGCTATTCTGGATGCTCTCCTATCAAAGCAGCTTGCTAGTGGAAAATTGTTTGTTGGACAGAAACTTCGGATTTGGGGAGCAGGACTCTGCGGCTGGGTTGGGCCCATTTCACCCCTCGAGGTGTCAAGAGAAGTTATGTTAAGGCTACACATAAATGGCACATATAGAGCTCATTGGGATGATCAATTGGGATTTTGCAACTGTGTTGGTGCTCCGTTAGCATTTAAGTTAAGTAATGGAAGATCTGTTGTGAGATCGGAGAGGTTGGAGACCAAAATGATGCAATCATATAGCCAGAGGCGCTCTAATGTCATTGAGGGCATTATTTCTGAGTTCCAAAGAGGATTAGAACATTCGCATGCATGTAATGAGAAAGATAGTGAAGGAGCAAAACTCTTGAGGATACTAGAGACAGCTGCTGAACCAGAAGTTTTAATGGCAGAGATGAGTACAGAACAATTGAATTCTTTTACCAAATATCGAGCAAAATTAGAGGCAATCAAGCAGTCAGACATGGACAAATCAATTATGAAAGCACTAGAAGATGCTGGATTAGCTGAGAGGGAAGTGATGCCATTAATGAGGGGTTTGTTCTGCAGTGAATTTGATATTGCTGCATTTGTTGTGTTTGTGGGGGAGGTTTATATCGCTGGTCACCAGAAGAAACAGTGGGTGTTTGTGACAGATGGCTCCATTTCTGAATCAAAGTCAGAAGAATTAAACGATTCTCTACTTGCCATCTGCTTCTGTTCACCGCATATCGATGATCAATCAGATGCTCCTATAAACTACAACCTCGCTGGGTCAACGGTCGGCTTTTGTAATCTTATCAAGAGAGCAAAGGACCAAATGAATGCTCTTTGGGTAGCTGAAGCTACAGAAAACTCGGCATACTTTTTGAGTTTTGACTCTCCACATTGTTCACATCTCAAAGGTGCTGCTGCCTCTGCTGAAAGATGGGGAAGAATCTCTAGCTTGACCATCAGTAGGCTTAAGGAGAGGGTTCTATTTATTATTGCAGTCTTGATATTGGAAACTAGAGTGCTGAGTGAACTCAAAAATAAGAATGCTGGTGTACAGTATTCCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000226 GO:0000228 GO:0000278 GO:0000280 GO:0000281 GO:0000722 GO:0000723 GO:0000724 GO:0000725 GO:0000781 GO:0000784 GO:0000793 GO:0000794 GO:0000795 GO:0000800 GO:0000910 GO:0001556 GO:0001701 GO:0001824 GO:0001832 GO:0001833 GO:0002020 GO:0002376 GO:0002520 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003697 GO:0003824 GO:0004402 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005739 GO:0005813 GO:0005815 GO:0005829 GO:0005856 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006275 GO:0006281 GO:0006289 GO:0006302 GO:0006310 GO:0006312 GO:0006325 GO:0006355 GO:0006464 GO:0006473 GO:0006475 GO:0006725 GO:0006807 GO:0006915 GO:0006950 GO:0006974 GO:0006978 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007059 GO:0007098 GO:0007127 GO:0007140 GO:0007141 GO:0007154 GO:0007165 GO:0007275 GO:0007276 GO:0007281 GO:0007283 GO:0007292 GO:0007346 GO:0007399 GO:0007417 GO:0007420 GO:0007548 GO:0007568 GO:0007569 GO:0008022 GO:0008080 GO:0008092 GO:0008104 GO:0008150 GO:0008152 GO:0008156 GO:0008219 GO:0008283 GO:0008285 GO:0008406 GO:0008585 GO:0008608 GO:0008630 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009790 GO:0009792 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0009994 GO:0010165 GO:0010212 GO:0010225 GO:0010332 GO:0010468 GO:0010484 GO:0010485 GO:0010556 GO:0010557 GO:0010558 GO:0010564 GO:0010604 GO:0010605 GO:0010628 GO:0012501 GO:0012505 GO:0015630 GO:0015631 GO:0016043 GO:0016407 GO:0016410 GO:0016569 GO:0016570 GO:0016573 GO:0016740 GO:0016746 GO:0016747 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019538 GO:0019899 GO:0019953 GO:0021700 GO:0022402 GO:0022412 GO:0022414 GO:0023052 GO:0030097 GO:0030141 GO:0030154 GO:0030330 GO:0030879 GO:0031023 GO:0031297 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031410 GO:0031619 GO:0031974 GO:0031981 GO:0031982 GO:0032200 GO:0032465 GO:0032501 GO:0032502 GO:0032504 GO:0032991 GO:0033036 GO:0033260 GO:0033365 GO:0033554 GO:0033593 GO:0033599 GO:0033600 GO:0034212 GO:0034502 GO:0034613 GO:0034641 GO:0034645 GO:0035264 GO:0035556 GO:0036211 GO:0040007 GO:0042127 GO:0042592 GO:0042770 GO:0042771 GO:0042772 GO:0042802 GO:0043009 GO:0043015 GO:0043060 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043543 GO:0043966 GO:0043967 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044454 GO:0044464 GO:0044703 GO:0044786 GO:0045005 GO:0045132 GO:0045137 GO:0045143 GO:0045184 GO:0045787 GO:0045893 GO:0045931 GO:0045934 GO:0045935 GO:0046483 GO:0046545 GO:0046660 GO:0048232 GO:0048285 GO:0048468 GO:0048469 GO:0048477 GO:0048478 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048534 GO:0048589 GO:0048599 GO:0048608 GO:0048609 GO:0048731 GO:0048732 GO:0048856 GO:0048869 GO:0050000 GO:0050678 GO:0050680 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051052 GO:0051053 GO:0051093 GO:0051171 GO:0051172 GO:0051173 GO:0051179 GO:0051234 GO:0051239 GO:0051241 GO:0051252 GO:0051254 GO:0051276 GO:0051298 GO:0051301 GO:0051302 GO:0051303 GO:0051310 GO:0051311 GO:0051316 GO:0051321 GO:0051455 GO:0051640 GO:0051641 GO:0051649 GO:0051656 GO:0051704 GO:0051716 GO:0051726 GO:0060249 GO:0060255 GO:0060322 GO:0061458 GO:0061640 GO:0061733 GO:0061982 GO:0065007 GO:0065008 GO:0070013 GO:0070198 GO:0070199 GO:0070200 GO:0070727 GO:0071695 GO:0071704 GO:0071840 GO:0072331 GO:0072332 GO:0072594 GO:0080090 GO:0090304 GO:0090329 GO:0097159 GO:0097190 GO:0097193 GO:0097708 GO:0098687 GO:0098813 GO:0099086 GO:0099503 GO:0140013 GO:1901360 GO:1901363 GO:1901564 GO:1901576 GO:1902298 GO:1902680 GO:1902969 GO:1903046 GO:1903047 GO:1903506 GO:1903508 GO:1990426 GO:1990505 GO:2000026 GO:2000104 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03440 ko03460 ko05200 ko05212 ko05224 map03440 map03460 map05200 map05212 map05224
Pfam Domains
Protein Families
BRCA-2_OB1 BRCA-2_helical BRCA2

Protein Analysis

987

Amino Acids

108.14

Weight (kDa)

9.27

Isoelectric Point (pI)

48.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BRCA2 PF00634 124 - 149 5.4e-06 BRCA2 repeat
BRCA-2_helical PF09169 485 - 561 3.7e-14 BRCA2, helical
BRCA-2_OB1 PF09103 569 - 690 5.5e-40 BRCA2, oligonucleotide/oligosaccharide-binding, domain 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 213
AasI GACNNNNNNGTC 1 cut(s) 1357
AbsI CCTCGAGG 1 cut(s) 1988
Acc36I ACCTGC 1 cut(s) 213
AccB1I GGYRCC 1 cut(s) 1142
AccB7I CCANNNNNTGG 2 cut(s) 1519, 1560
AccBSI CCGCTC 1 cut(s) 1704
AccII CGCG 1 cut(s) 195
AccIII TCCGGA 2 cut(s) 1529, 1811
AclWI GGATC 3 cut(s) 250, 667, 969
AcoI YGGCCR 1 cut(s) 160
AcsI RAATTY 5 cut(s) 586, 1198, 1640, 2335, 2474
AcuI CTGAAG 3 cut(s) 621, 1000, 2763
AcyI GRCGYC 3 cut(s) 27, 91, 294
AfaI GTAC 7 cut(s) 613, 622, 778, 1033, 1330, 2323, 2949
AfiI CCNNNNNNNGG 8 cut(s) 86, 656, 925, 926, 943, 1519, 1560, 2859
AflII CTTAAG 1 cut(s) 2864
AflIII ACRYGT 3 cut(s) 15, 1461, 1515
AgeI ACCGGT 1 cut(s) 479
AhdI GACNNNNNGTC 1 cut(s) 1414
AjnI CCWGG 2 cut(s) 805, 1344
AjuI GAANNNNNNNTTGG 2 cut(s) 941, 973
Alw21I GWGCWC 4 cut(s) 309, 841, 2044, 2086
Alw26I GTCTC 3 cut(s) 1796, 2135, 2276
Alw44I GTGCAC 1 cut(s) 837
AlwI GGATC 3 cut(s) 250, 667, 969
AlwNI CAGNNNCTG 2 cut(s) 698, 2291
Ama87I CYCGRG 1 cut(s) 1988
Aor13HI TCCGGA 2 cut(s) 1529, 1811
AoxI GGCC 4 cut(s) 160, 322, 429, 1973
ApaI GGGCCC 1 cut(s) 1977
ApaLI GTGCAC 1 cut(s) 837
ApeKI GCWGC 5 cut(s) 1900, 2288, 2486, 2810, 2813
ApoI RAATTY 5 cut(s) 586, 1198, 1640, 2335, 2474
ArsI GACNNNNNNTTYG 4 cut(s) 408, 440, 1920, 1952
AseI ATTAAT 3 cut(s) 1086, 1446, 2450
AsiGI ACCGGT 1 cut(s) 479
AspLEI GCGC 3 cut(s) 55, 556, 2175
AspS9I GGNCC 3 cut(s) 1973, 1974, 2716
AsuC2I CCSGG 2 cut(s) 47, 651
AvaI CYCGRG 1 cut(s) 1988
AvaII GGWCC 1 cut(s) 2716
BaeGI GKGCMC 2 cut(s) 841, 1977
BanI GGYRCC 1 cut(s) 1142
BanII GRGCYC 3 cut(s) 309, 1977, 2044
BarI GAAGNNNNNNTAC 2 cut(s) 1312, 1344
BbsI GAAGAC 1 cut(s) 899
Bbv12I GWGCWC 4 cut(s) 309, 841, 2044, 2086
BbvI GCAGC 5 cut(s) 1912, 2275, 2473, 2797, 2800
BccI CCATC 7 cut(s) 391, 1396, 1847, 2552, 2618, 2823, 2861
BceAI ACGGC 1 cut(s) 257
BcgI CGANNNNNNTGC 2 cut(s) 230, 264
BciT130I CCWGG 2 cut(s) 807, 1346
BciVI GTATCC 1 cut(s) 2268
BclI TGATCA 2 cut(s) 2053, 2638
BcnI CCSGG 2 cut(s) 47, 651
BcoDI GTCTC 3 cut(s) 1796, 2135, 2276
BfmI CTRYAG 3 cut(s) 1041, 2467, 2748
BfoI RGCGCY 1 cut(s) 2176
BfrI CTTAAG 1 cut(s) 2864
BfuAI ACCTGC 1 cut(s) 213
BfuI GTATCC 1 cut(s) 2268
BglI GCCNNNNNGGC 1 cut(s) 291
BglII AGATCT 2 cut(s) 865, 2114
Bme1390I CCNGG 4 cut(s) 47, 651, 807, 1346
Bme18I GGWCC 1 cut(s) 2716
BmeRI GACNNNNNGTC 1 cut(s) 1414
BmeT110I CYCGRG 1 cut(s) 1988
BmgT120I GGNCC 3 cut(s) 1973, 1974, 2716
BmiI GGNNCC 5 cut(s) 65, 1144, 1534, 1975, 2563
BmrFI CCNGG 4 cut(s) 47, 651, 807, 1346
BmrI ACTGGG 2 cut(s) 504, 570
BmuI ACTGGG 2 cut(s) 504, 570
BpiI GAAGAC 1 cut(s) 899
BplI GAGNNNNNCTC 8 cut(s) 935, 967, 1793, 1825, 1941, 1973, 2250, 2282
BpmI CTGGAG 2 cut(s) 828, 878
BpuEI CTTGAG 4 cut(s) 110, 116, 122, 2290
BpuMI CCSGG 2 cut(s) 47, 651
Bsa29I ATCGAT 1 cut(s) 2634
BsaAI YACGTR 1 cut(s) 1492
BsaHI GRCGYC 3 cut(s) 27, 91, 294
BsaI GGTCTC 1 cut(s) 2135
BsaJI CCNNGG 2 cut(s) 157, 1401
BsaWI WCCGGW 3 cut(s) 479, 1529, 1811
BsaXI ACNNNNNCTCC 2 cut(s) 5, 35
Bsc4I CCNNNNNNNGG 8 cut(s) 86, 656, 925, 926, 943, 1519, 1560, 2859
Bse118I RCCGGY 2 cut(s) 29, 479
Bse1I ACTGG 8 cut(s) 499, 550, 565, 838, 853, 1078, 1602, 1728
Bse3DI GCAATG 2 cut(s) 248, 1247
BseAI TCCGGA 2 cut(s) 1529, 1811
BseBI CCWGG 2 cut(s) 807, 1346
BseCI ATCGAT 1 cut(s) 2634
BseDI CCNNGG 2 cut(s) 157, 1401
BseGI GGATG 4 cut(s) 511, 1876, 1888, 2056
BseLI CCNNNNNNNGG 8 cut(s) 86, 656, 925, 926, 943, 1519, 1560, 2859
BseMI GCAATG 2 cut(s) 248, 1247
BseMII CTCAG 7 cut(s) 134, 294, 966, 1145, 2193, 2421, 2909
BseNI ACTGG 8 cut(s) 499, 550, 565, 838, 853, 1078, 1602, 1728
BseRI GAGGAG 2 cut(s) 1140, 1198
BseSI GKGCMC 2 cut(s) 841, 1977
BseXI GCAGC 5 cut(s) 1912, 2275, 2473, 2797, 2800
BseYI CCCAGC 2 cut(s) 1965, 2672
BsgI GTGCAG 1 cut(s) 722
Bsh1236I CGCG 1 cut(s) 195
Bsh1285I CGRYCG 1 cut(s) 2685
BshFI GGCC 4 cut(s) 162, 324, 431, 1975
BshNI GGYRCC 1 cut(s) 1142
BshTI ACCGGT 1 cut(s) 479
BshVI ATCGAT 1 cut(s) 2634
BsiEI CGRYCG 1 cut(s) 2685
BsiHKAI GWGCWC 4 cut(s) 309, 841, 2044, 2086
BsiHKCI CYCGRG 1 cut(s) 1988
BsiSI CCGG 7 cut(s) 30, 47, 87, 480, 651, 1530, 1812
BslI CCNNNNNNNGG 8 cut(s) 86, 656, 925, 926, 943, 1519, 1560, 2859
BsmAI GTCTC 3 cut(s) 1796, 2135, 2276
BsmI GAATGC 3 cut(s) 1386, 2731, 2944
BsnI GGCC 4 cut(s) 162, 324, 431, 1975
Bso31I GGTCTC 1 cut(s) 2135
BsoBI CYCGRG 1 cut(s) 1988
Bsp120I GGGCCC 1 cut(s) 1973
Bsp1286I GDGCHC 5 cut(s) 309, 841, 1977, 2044, 2086
Bsp13I TCCGGA 2 cut(s) 1529, 1811
Bsp1407I TGTACA 2 cut(s) 1328, 2947
Bsp19I CCATGG 2 cut(s) 157, 1401
BspANI GGCC 4 cut(s) 162, 324, 431, 1975
BspCNI CTCAG 7 cut(s) 133, 295, 965, 1146, 2194, 2422, 2910
BspDI ATCGAT 1 cut(s) 2634
BspEI TCCGGA 2 cut(s) 1529, 1811
BspFNI CGCG 1 cut(s) 195
BspLI GGNNCC 5 cut(s) 65, 1144, 1534, 1975, 2563
BspMAI CTGCAG 1 cut(s) 2471
BspMI ACCTGC 1 cut(s) 213
BspPI GGATC 3 cut(s) 250, 667, 969
BspT107I GGYRCC 1 cut(s) 1142
BspTI CTTAAG 1 cut(s) 2864
BspTNI GGTCTC 1 cut(s) 2135
BsrBI CCGCTC 1 cut(s) 1704
BsrDI GCAATG 2 cut(s) 248, 1247
BsrFI RCCGGY 2 cut(s) 29, 479
BsrGI TGTACA 2 cut(s) 1328, 2947
BsrI ACTGG 8 cut(s) 499, 550, 565, 838, 853, 1078, 1602, 1728
BssAI RCCGGY 2 cut(s) 29, 479
BssECI CCNNGG 2 cut(s) 157, 1401
BssNI GRCGYC 3 cut(s) 27, 91, 294
BssT1I CCWWGG 2 cut(s) 157, 1401
Bst2UI CCWGG 2 cut(s) 807, 1346
Bst4CI ACNGT 6 cut(s) 470, 1045, 2075, 2541, 2683, 2952
Bst6I CTCTTC 1 cut(s) 1426
BstACI GRCGYC 3 cut(s) 27, 91, 294
BstAFI CTTAAG 1 cut(s) 2864
BstAPI GCANNNNNTGC 2 cut(s) 228, 1448
BstAUI TGTACA 2 cut(s) 1328, 2947
BstBAI YACGTR 1 cut(s) 1492
BstC8I GCNNGC 5 cut(s) 31, 150, 597, 1905, 2232
BstDEI CTNAG 7 cut(s) 120, 303, 952, 1154, 2202, 2430, 2918
BstDSI CCRYGG 2 cut(s) 157, 1401
BstEII GGTNACC 4 cut(s) 914, 1348, 1697, 2525
BstENI CCTNNNNNAGG 1 cut(s) 941
BstF5I GGATG 4 cut(s) 511, 1876, 1888, 2056
BstFNI CGCG 1 cut(s) 195
BstH2I RGCGCY 1 cut(s) 2176
BstHHI GCGC 3 cut(s) 55, 556, 2175
BstMAI GTCTC 3 cut(s) 1796, 2135, 2276
BstMCI CGRYCG 1 cut(s) 2685
BstMWI GCNNNNNNNGC 9 cut(s) 228, 291, 760, 1448, 1660, 2090, 2172, 2426, 2819
BstNI CCWGG 2 cut(s) 807, 1346
BstNSI RCATGY 6 cut(s) 19, 1417, 1465, 1519, 2234, 2238
BstPI GGTNACC 4 cut(s) 914, 1348, 1697, 2525
BstSCI CCNGG 4 cut(s) 45, 649, 805, 1344
BstSFI CTRYAG 3 cut(s) 1041, 2467, 2748
BstSLI GKGCMC 2 cut(s) 841, 1977
BstSNI TACGTA 1 cut(s) 1492
BstUI CGCG 1 cut(s) 195
BstV1I GCAGC 5 cut(s) 1912, 2275, 2473, 2797, 2800
BstV2I GAAGAC 1 cut(s) 899
BstX2I RGATCY 5 cut(s) 255, 659, 865, 961, 2114
BstXI CCANNNNNNTGG 2 cut(s) 1117, 1310
BstYI RGATCY 5 cut(s) 255, 659, 865, 961, 2114
Bsu15I ATCGAT 1 cut(s) 2634
BsuI GTATCC 1 cut(s) 2268
BsuRI GGCC 4 cut(s) 162, 324, 431, 1975
BsuTUI ATCGAT 1 cut(s) 2634
BtgI CCRYGG 2 cut(s) 157, 1401
BtsCI GGATG 4 cut(s) 511, 1876, 1888, 2056
BtsI GCAGTG 2 cut(s) 1649, 2476
BtsIMutI CAGTG 7 cut(s) 831, 879, 1071, 1649, 1872, 2476, 2546
BveI ACCTGC 1 cut(s) 213
Cac8I GCNNGC 5 cut(s) 31, 150, 597, 1905, 2232
CaiI CAGNNNCTG 2 cut(s) 698, 2291
CfoI GCGC 3 cut(s) 55, 556, 2175
Cfr10I RCCGGY 2 cut(s) 29, 479
Cfr13I GGNCC 3 cut(s) 1973, 1974, 2716
ClaI ATCGAT 1 cut(s) 2634
CseI GACGC 4 cut(s) 35, 99, 228, 283
Csp6I GTAC 7 cut(s) 612, 621, 777, 1032, 1329, 2322, 2948
CspAI ACCGGT 1 cut(s) 479
CspCI CAANNNNNGTGG 2 cut(s) 1822, 1857
CviQI GTAC 7 cut(s) 612, 621, 777, 1032, 1329, 2322, 2948
DdeI CTNAG 7 cut(s) 120, 303, 952, 1154, 2202, 2430, 2918
DrdI GACNNNNNNGTC 1 cut(s) 1357
DriI GACNNNNNGTC 1 cut(s) 1414
DseDI GACNNNNNNGTC 1 cut(s) 1357
EaeI YGGCCR 1 cut(s) 160
Eam1104I CTCTTC 1 cut(s) 1426
Eam1105I GACNNNNNGTC 1 cut(s) 1414
EarI CTCTTC 1 cut(s) 1426
EciI GGCGGA 1 cut(s) 213
Ecl136II GAGCTC 2 cut(s) 307, 2042
Eco105I TACGTA 1 cut(s) 1492
Eco130I CCWWGG 2 cut(s) 157, 1401
Eco24I GRGCYC 3 cut(s) 309, 1977, 2044
Eco31I GGTCTC 1 cut(s) 2135
Eco32I GATATC 1 cut(s) 1337
Eco47I GGWCC 1 cut(s) 2716
Eco53kI GAGCTC 2 cut(s) 307, 2042
Eco57I CTGAAG 3 cut(s) 621, 1000, 2763
Eco88I CYCGRG 1 cut(s) 1988
Eco91I GGTNACC 4 cut(s) 914, 1348, 1697, 2525
EcoICRI GAGCTC 2 cut(s) 307, 2042
EcoNI CCTNNNNNAGG 1 cut(s) 941
EcoO65I GGTNACC 4 cut(s) 914, 1348, 1697, 2525
EcoRI GAATTC 1 cut(s) 2335
EcoRII CCWGG 2 cut(s) 805, 1344
EcoRV GATATC 1 cut(s) 1337
EcoT14I CCWWGG 2 cut(s) 157, 1401
EcoT22I ATGCAT 4 cut(s) 685, 1738, 1766, 2236
EcoT38I GRGCYC 3 cut(s) 309, 1977, 2044
ErhI CCWWGG 2 cut(s) 157, 1401
FalI AAGNNNNNCTT 2 cut(s) 1830, 1862
FbaI TGATCA 2 cut(s) 2053, 2638
FokI GGATG 4 cut(s) 518, 1883, 1895, 2063
FriOI GRGCYC 3 cut(s) 309, 1977, 2044
GlaI GCGC 3 cut(s) 54, 555, 2174
GsaI CCCAGC 2 cut(s) 1969, 2676
GsuI CTGGAG 2 cut(s) 828, 878
HaeII RGCGCY 1 cut(s) 2176
HaeIII GGCC 4 cut(s) 162, 324, 431, 1975
HapII CCGG 7 cut(s) 30, 47, 87, 480, 651, 1530, 1812
HgaI GACGC 4 cut(s) 35, 99, 228, 283
HhaI GCGC 3 cut(s) 55, 556, 2175
Hin1I GRCGYC 3 cut(s) 27, 91, 294
Hin6I GCGC 3 cut(s) 53, 554, 2173
HinP1I GCGC 3 cut(s) 53, 554, 2173
HincII GTYRAC 2 cut(s) 567, 2679
HindII GTYRAC 2 cut(s) 567, 2679
HindIII AAGCTT 1 cut(s) 1629
HpaI GTTAAC 1 cut(s) 567
HpaII CCGG 7 cut(s) 30, 47, 87, 480, 651, 1530, 1812
Hpy99I CGWCG 5 cut(s) 29, 222, 278, 281, 299
HpyAV CCTTC 9 cut(s) 107, 163, 636, 792, 1024, 1314, 1721, 1752, 2249
HpyCH4III ACNGT 6 cut(s) 470, 1045, 2075, 2541, 2683, 2952
HpyCH4IV ACGT 2 cut(s) 895, 1491
HpyF10VI GCNNNNNNNGC 9 cut(s) 228, 291, 760, 1448, 1660, 2090, 2172, 2426, 2819
HpyF3I CTNAG 7 cut(s) 120, 303, 952, 1154, 2202, 2430, 2918
HpySE526I ACGT 2 cut(s) 895, 1491
Hsp92I GRCGYC 3 cut(s) 27, 91, 294
HspAI GCGC 3 cut(s) 53, 554, 2173
Kpn2I TCCGGA 2 cut(s) 1529, 1811
KroI GCCGGC 1 cut(s) 29
KroNI GCCGGC 1 cut(s) 31
Ksp22I TGATCA 2 cut(s) 2053, 2638
KspAI GTTAAC 1 cut(s) 567
LmnI GCTCC 9 cut(s) 312, 1532, 1814, 1814, 1949, 2089, 2258, 2567, 2656
Lsp1109I GCAGC 5 cut(s) 1912, 2275, 2473, 2797, 2800
MaeII ACGT 2 cut(s) 895, 1491
MbiI CCGCTC 1 cut(s) 1704
MfeI CAATTG 2 cut(s) 2057, 2330
MflI RGATCY 5 cut(s) 255, 659, 865, 961, 2114
MhlI GDGCHC 5 cut(s) 309, 841, 1977, 2044, 2086
MlyI GAGTC 5 cut(s) 212, 743, 913, 1950, 2774
MmeI TCCRAC 5 cut(s) 48, 91, 1257, 1906, 2118
Mph1103I ATGCAT 4 cut(s) 685, 1738, 1766, 2236
MreI CGCCGGCG 1 cut(s) 29
MroI TCCGGA 2 cut(s) 1529, 1811
MroNI GCCGGC 1 cut(s) 29
MslI CAYNNNNRTG 6 cut(s) 1257, 1308, 2025, 2049, 2229, 2634
MspA1I CMGCKG 1 cut(s) 2288
MspCI CTTAAG 1 cut(s) 2864
MspI CCGG 7 cut(s) 30, 47, 87, 480, 651, 1530, 1812
MspR9I CCNGG 4 cut(s) 47, 651, 807, 1346
MunI CAATTG 2 cut(s) 2057, 2330
Mva1269I GAATGC 3 cut(s) 1386, 2731, 2944
MvaI CCWGG 2 cut(s) 807, 1346
MvnI CGCG 1 cut(s) 195
MwoI GCNNNNNNNGC 9 cut(s) 228, 291, 760, 1448, 1660, 2090, 2172, 2426, 2819
NaeI GCCGGC 1 cut(s) 31
NciI CCSGG 2 cut(s) 47, 651
NcoI CCATGG 2 cut(s) 157, 1401
NgoMIV GCCGGC 1 cut(s) 29
NlaIV GGNNCC 5 cut(s) 65, 1144, 1534, 1975, 2563
NmeAIII GCCGAG 1 cut(s) 2738
NmuCI GTSAC 7 cut(s) 464, 914, 1101, 1348, 1697, 2525, 2551
NsiI ATGCAT 4 cut(s) 685, 1738, 1766, 2236
NspI RCATGY 6 cut(s) 19, 1417, 1465, 1519, 2234, 2238
PaeI GCATGC 1 cut(s) 2234
PaeR7I CTCGAG 1 cut(s) 1988
PaqCI CACCTGC 1 cut(s) 213
PciI ACATGT 3 cut(s) 15, 1461, 1515
PcsI WCGNNNNNNNCGW 1 cut(s) 256
PctI GAATGC 3 cut(s) 1386, 2731, 2944
PdiI GCCGGC 1 cut(s) 31
PfeI GAWTC 9 cut(s) 247, 312, 368, 535, 1475, 1690, 2573, 2597, 2838
PflMI CCANNNNNTGG 2 cut(s) 1519, 1560
PinAI ACCGGT 1 cut(s) 479
PleI GAGTC 5 cut(s) 211, 742, 912, 1950, 2774
PpsI GAGTC 5 cut(s) 211, 742, 912, 1950, 2774
Ppu21I YACGTR 1 cut(s) 1492
PscI ACATGT 3 cut(s) 15, 1461, 1515
PshBI ATTAAT 3 cut(s) 1086, 1446, 2450
Psp124BI GAGCTC 2 cut(s) 309, 2044
Psp6I CCWGG 2 cut(s) 805, 1344
PspEI GGTNACC 4 cut(s) 914, 1348, 1697, 2525
PspFI CCCAGC 2 cut(s) 1965, 2672
PspGI CCWGG 2 cut(s) 805, 1344
PspN4I GGNNCC 5 cut(s) 65, 1144, 1534, 1975, 2563
PspOMI GGGCCC 1 cut(s) 1973
PspPI GGNCC 3 cut(s) 1973, 1974, 2716
PspXI VCTCGAGB 1 cut(s) 1988
PstI CTGCAG 1 cut(s) 2471
PstNI CAGNNNCTG 2 cut(s) 698, 2291
PsuI RGATCY 5 cut(s) 255, 659, 865, 961, 2114
PvuII CAGCTG 1 cut(s) 2288
RsaI GTAC 7 cut(s) 613, 622, 778, 1033, 1330, 2323, 2949
RsaNI GTAC 7 cut(s) 612, 621, 777, 1032, 1329, 2322, 2948
RseI CAYNNNNRTG 6 cut(s) 1257, 1308, 2025, 2049, 2229, 2634
SacI GAGCTC 2 cut(s) 309, 2044
Sau96I GGNCC 3 cut(s) 1973, 1974, 2716
SchI GAGTC 5 cut(s) 212, 743, 913, 1950, 2774
ScrFI CCNGG 4 cut(s) 47, 651, 807, 1346
SduI GDGCHC 5 cut(s) 309, 841, 1977, 2044, 2086
SfcI CTRYAG 3 cut(s) 1041, 2467, 2748
Sfr274I CTCGAG 1 cut(s) 1988
SgrAI CRCCGGYG 1 cut(s) 29
SinI GGWCC 1 cut(s) 2716
SlaI CTCGAG 1 cut(s) 1988
SmiMI CAYNNNNRTG 6 cut(s) 1257, 1308, 2025, 2049, 2229, 2634
SmlI CTYRAG 6 cut(s) 125, 131, 137, 1988, 2269, 2864
SmoI CTYRAG 6 cut(s) 125, 131, 137, 1988, 2269, 2864
SnaBI TACGTA 1 cut(s) 1492
SphI GCATGC 1 cut(s) 2234
SspI AATATT 1 cut(s) 1373
SstI GAGCTC 2 cut(s) 309, 2044
StyD4I CCNGG 4 cut(s) 45, 649, 805, 1344
StyI CCWWGG 2 cut(s) 157, 1401
TaaI ACNGT 6 cut(s) 470, 1045, 2075, 2541, 2683, 2952
TaiI ACGT 2 cut(s) 898, 1494
TaqI TCGA 3 cut(s) 1989, 2353, 2634
TatI WGTACW 4 cut(s) 611, 1328, 2321, 2947
TauI GCSGC 6 cut(s) 36, 39, 96, 198, 294, 1965
TfiI GAWTC 9 cut(s) 247, 312, 368, 535, 1475, 1690, 2573, 2597, 2838
TscAI CASTG 7 cut(s) 838, 886, 1078, 1656, 1872, 2476, 2546
TseFI GTSAC 7 cut(s) 464, 914, 1101, 1348, 1697, 2525, 2551
TseI GCWGC 5 cut(s) 1900, 2288, 2486, 2810, 2813
Tsp45I GTSAC 7 cut(s) 464, 914, 1101, 1348, 1697, 2525, 2551
TspDTI ATGAA 6 cut(s) 344, 618, 774, 1488, 2417, 2738
TspGWI ACGGA 4 cut(s) 248, 254, 1284, 2076
TspRI CASTG 7 cut(s) 838, 886, 1078, 1656, 1872, 2476, 2546
Van91I CCANNNNNTGG 2 cut(s) 1519, 1560
Vha464I CTTAAG 1 cut(s) 2864
VneI GTGCAC 1 cut(s) 837
VpaK11BI GGWCC 1 cut(s) 2716
VspI ATTAAT 3 cut(s) 1086, 1446, 2450
XagI CCTNNNNNAGG 1 cut(s) 941
XapI RAATTY 5 cut(s) 586, 1198, 1640, 2335, 2474
XceI RCATGY 6 cut(s) 19, 1417, 1465, 1519, 2234, 2238
XcmI CCANNNNNNNNNTGG 2 cut(s) 386, 1117
XhoI CTCGAG 1 cut(s) 1988
Zsp2I ATGCAT 4 cut(s) 685, 1738, 1766, 2236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.