AT5G01700

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009446
Physical Location & Seq
Reverse (-)
260723 .. 263451
2729 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G01700.2

Sequence Viewer

Length: 1002 bp
ATGTCAATCAAACAAGGGAAAAAAGGAATAAATCAAGACGCCATGACAGTCTGGGAGAACTTTGGTGGGGAAGAAGATACGATCTTTTGCGGTGTATTTGACGGTCACGGTCCCATGGGTCACAAGATTTCTCGTCACGTATGTGAAAATTTACCTTCGAGGGTCCATTCAAAAATTAGATCTTCCAAATCTGCCGGCGATGAAAACATAGAGAATAATAGTTCACAGAGTCAGGAGGAACTCTTCCGTGAATTCGAGGACATTCTAGTGACATTCTTCAAGCAAATAGACAGTGAACTTGGTCTCGACTCTCCTTATGATAGTTTTTGCAGCGGTACAACTGCTGTAACTGTCTTTAAACAGGCTGACTGTTTGGTGATCGCCAACTTGGGACATTCACGAGCCGTTCTTGGCACCCGAAGCAAGAACAGTTTCAAAGCTGTCCAACTCACGGTTGATCTGAAACCCTGTGTCCAACGCGAAGCAGAGAGAATAGTGTCATGCAAGGGAAGGGTGTTTGCTATGGAAGAGGAACCCGATGTGTATAGAGTGTGGATGCCTGATGATGATTGCCCGGGGCTCGCAATGTCAAGGGCTTTTGGTGATTTCTGCCTCAAAGACTATGGACTTGTCTGCATTCCTGACGTCTTCTGCAGAAAAGTCAGCAGAGAAGACGAGTTTGTGGTTCTAGCTACTGATGGGATTTGGGATGTTTTGTCAAACGAAGAAGTGGTGAAAGTTGTAGGTTCCTGCAAGGACCGGTCAGTTGCAGCTGAGATGTTGGTTCAGCGAGCCGCTCGGACTTGGAGAACAAAGTTTCCAGCCTCAAAAGCTGATGACTGTGCGGTGGTGGTGCTTTACCTAAACCACCGGCCTTACCCAAGAGAAGGAAACGTGAGCCGAGCAATATCAACTATTTCCTGGAGATCAAATAAGAGCAATAACGAGTGTTATGGAGCCGCACCATTGTCACCATTGGGTCTATCCCAAAGGGTTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

333

Amino Acids

37.04

Weight (kDa)

5.79

Isoelectric Point (pI)

45.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 60 - 315 1.1e-36 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 648
AccB1I GGYRCC 1 cut(s) 413
AccBSI CCGCTC 1 cut(s) 797
AccII CGCG 1 cut(s) 480
AciI CCGC 5 cut(s) 90, 333, 795, 845, 960
AcsI RAATTY 2 cut(s) 148, 251
AcyI GRCGYC 2 cut(s) 39, 645
AfaI GTAC 1 cut(s) 337
AfiI CCNNNNNNNGG 2 cut(s) 451, 887
AgeI ACCGGT 1 cut(s) 759
AgsI TTSAA 3 cut(s) 171, 280, 436
AjnI CCWGG 1 cut(s) 920
AleI CACNNNNGTG 1 cut(s) 141
AloI GAACNNNNNNTCC 2 cut(s) 802, 834
AluBI AGCT 4 cut(s) 440, 692, 773, 833
AluI AGCT 4 cut(s) 440, 692, 773, 833
Alw26I GTCTC 1 cut(s) 308
Ama87I CYCGRG 1 cut(s) 574
AoxI GGCC 1 cut(s) 872
ApeKI GCWGC 2 cut(s) 330, 770
ApoI RAATTY 2 cut(s) 148, 251
AsiGI ACCGGT 1 cut(s) 759
Asp700I GAANNNNTTC 1 cut(s) 431
AspS9I GGNCC 3 cut(s) 110, 163, 757
AsuC2I CCSGG 2 cut(s) 575, 576
AsuHPI GGTGA 4 cut(s) 388, 614, 745, 963
AvaI CYCGRG 1 cut(s) 574
AvaII GGWCC 3 cut(s) 110, 163, 757
BanI GGYRCC 1 cut(s) 413
BanII GRGCYC 1 cut(s) 582
BauI CACGAG 1 cut(s) 399
BbsI GAAGAC 2 cut(s) 640, 678
BbvI GCAGC 2 cut(s) 342, 782
BccI CCATC 1 cut(s) 692
BceAI ACGGC 1 cut(s) 389
BciT130I CCWGG 1 cut(s) 922
BcnI CCSGG 2 cut(s) 575, 576
BcoDI GTCTC 1 cut(s) 308
BfaI CTAG 2 cut(s) 266, 689
BfmI CTRYAG 1 cut(s) 652
BglII AGATCT 1 cut(s) 179
BisI GCNGC 4 cut(s) 331, 771, 795, 960
BlsI GCNGC 4 cut(s) 332, 772, 796, 961
Bme1390I CCNGG 3 cut(s) 575, 576, 922
Bme18I GGWCC 3 cut(s) 110, 163, 757
BmeT110I CYCGRG 1 cut(s) 574
BmgT120I GGNCC 3 cut(s) 110, 163, 757
BmiI GGNNCC 6 cut(s) 112, 164, 415, 534, 748, 958
BmrFI CCNGG 3 cut(s) 575, 576, 922
BmsI GCATC 1 cut(s) 546
BpiI GAAGAC 2 cut(s) 640, 678
BpmI CTGGAG 1 cut(s) 943
BpuMI CCSGG 2 cut(s) 575, 576
BsaAI YACGTR 1 cut(s) 139
BsaHI GRCGYC 2 cut(s) 39, 645
BsaI GGTCTC 1 cut(s) 308
BsaJI CCNNGG 3 cut(s) 114, 574, 575
BsaWI WCCGGW 1 cut(s) 759
Bsc4I CCNNNNNNNGG 2 cut(s) 451, 887
Bse118I RCCGGY 3 cut(s) 194, 759, 870
Bse3DI GCAATG 1 cut(s) 591
BseBI CCWGG 1 cut(s) 922
BseDI CCNNGG 3 cut(s) 114, 574, 575
BseGI GGATG 2 cut(s) 561, 715
BseLI CCNNNNNNNGG 2 cut(s) 451, 887
BseMI GCAATG 1 cut(s) 591
BseMII CTCAG 1 cut(s) 765
BseXI GCAGC 2 cut(s) 342, 782
Bsh1236I CGCG 1 cut(s) 480
BshFI GGCC 1 cut(s) 874
BshNI GGYRCC 1 cut(s) 413
BshTI ACCGGT 1 cut(s) 759
BsiHKCI CYCGRG 1 cut(s) 574
BsiSI CCGG 4 cut(s) 195, 575, 760, 871
BslFI GGGAC 2 cut(s) 96, 405
BslI CCNNNNNNNGG 2 cut(s) 451, 887
BsmAI GTCTC 1 cut(s) 308
BsmFI GGGAC 2 cut(s) 96, 405
BsmI GAATGC 1 cut(s) 636
BsnI GGCC 1 cut(s) 874
Bso31I GGTCTC 1 cut(s) 308
BsoBI CYCGRG 1 cut(s) 574
Bsp1286I GDGCHC 1 cut(s) 582
Bsp143I GATC 5 cut(s) 81, 179, 378, 457, 926
Bsp19I CCATGG 1 cut(s) 114
BspACI CCGC 5 cut(s) 90, 333, 795, 845, 960
BspANI GGCC 1 cut(s) 874
BspCNI CTCAG 1 cut(s) 766
BspFNI CGCG 1 cut(s) 480
BspLI GGNNCC 6 cut(s) 112, 164, 415, 534, 748, 958
BspMAI CTGCAG 1 cut(s) 656
BspT107I GGYRCC 1 cut(s) 413
BspTNI GGTCTC 1 cut(s) 308
BsrBI CCGCTC 1 cut(s) 797
BsrDI GCAATG 1 cut(s) 591
BsrFI RCCGGY 3 cut(s) 194, 759, 870
BssAI RCCGGY 3 cut(s) 194, 759, 870
BssECI CCNNGG 3 cut(s) 114, 574, 575
BssMI GATC 5 cut(s) 81, 179, 378, 457, 926
BssNI GRCGYC 2 cut(s) 39, 645
BssSI CACGAG 1 cut(s) 399
BssT1I CCWWGG 1 cut(s) 114
Bst2BI CACGAG 1 cut(s) 399
Bst2UI CCWGG 1 cut(s) 922
Bst4CI ACNGT 9 cut(s) 49, 104, 110, 293, 352, 371, 431, 454, 842
Bst6I CTCTTC 2 cut(s) 248, 522
BstACI GRCGYC 2 cut(s) 39, 645
BstBAI YACGTR 1 cut(s) 139
BstC8I GCNNGC 3 cut(s) 196, 582, 792
BstDEI CTNAG 1 cut(s) 774
BstDSI CCRYGG 1 cut(s) 114
BstF5I GGATG 2 cut(s) 561, 715
BstFNI CGCG 1 cut(s) 480
BstKTI GATC 5 cut(s) 84, 182, 381, 460, 929
BstMAI GTCTC 1 cut(s) 308
BstMBI GATC 5 cut(s) 81, 179, 378, 457, 926
BstMWI GCNNNNNNNGC 2 cut(s) 420, 830
BstNI CCWGG 1 cut(s) 922
BstSCI CCNGG 3 cut(s) 573, 574, 920
BstSFI CTRYAG 1 cut(s) 652
BstUI CGCG 1 cut(s) 480
BstV1I GCAGC 2 cut(s) 342, 782
BstV2I GAAGAC 2 cut(s) 640, 678
BstX2I RGATCY 1 cut(s) 179
BstYI RGATCY 1 cut(s) 179
BsuRI GGCC 1 cut(s) 874
BtgI CCRYGG 1 cut(s) 114
BtgZI GCGATG 1 cut(s) 213
BtsCI GGATG 2 cut(s) 561, 715
BtsIMutI CAGTG 1 cut(s) 298
Cac8I GCNNGC 3 cut(s) 196, 582, 792
Cfr10I RCCGGY 3 cut(s) 194, 759, 870
Cfr13I GGNCC 3 cut(s) 110, 163, 757
Cfr9I CCCGGG 1 cut(s) 574
CseI GACGC 1 cut(s) 47
Csp6I GTAC 1 cut(s) 336
CspAI ACCGGT 1 cut(s) 759
CviAII CATG 3 cut(s) 43, 115, 501
CviQI GTAC 1 cut(s) 336
DdeI CTNAG 1 cut(s) 774
DpnI GATC 5 cut(s) 83, 181, 380, 459, 928
DpnII GATC 5 cut(s) 81, 179, 378, 457, 926
DraI TTTAAA 1 cut(s) 358
Eam1104I CTCTTC 2 cut(s) 248, 522
EarI CTCTTC 2 cut(s) 248, 522
Eco130I CCWWGG 1 cut(s) 114
Eco24I GRGCYC 1 cut(s) 582
Eco31I GGTCTC 1 cut(s) 308
Eco47I GGWCC 3 cut(s) 110, 163, 757
Eco88I CYCGRG 1 cut(s) 574
EcoRI GAATTC 1 cut(s) 251
EcoRII CCWGG 1 cut(s) 920
EcoT14I CCWWGG 1 cut(s) 114
EcoT38I GRGCYC 1 cut(s) 582
ErhI CCWWGG 1 cut(s) 114
FaeI CATG 3 cut(s) 46, 118, 504
FaqI GGGAC 2 cut(s) 96, 405
FatI CATG 3 cut(s) 42, 114, 500
Fnu4HI GCNGC 4 cut(s) 331, 771, 795, 960
FokI GGATG 2 cut(s) 568, 722
FriOI GRGCYC 1 cut(s) 582
Fsp4HI GCNGC 4 cut(s) 331, 771, 795, 960
FspBI CTAG 2 cut(s) 266, 689
GluI GCNGC 4 cut(s) 331, 771, 795, 960
GsuI CTGGAG 1 cut(s) 943
HaeIII GGCC 1 cut(s) 874
HapII CCGG 4 cut(s) 195, 575, 760, 871
HgaI GACGC 1 cut(s) 47
Hin1I GRCGYC 2 cut(s) 39, 645
Hin1II CATG 3 cut(s) 46, 118, 504
HinfI GANTC 2 cut(s) 229, 308
HpaII CCGG 4 cut(s) 195, 575, 760, 871
HphI GGTGA 4 cut(s) 388, 614, 745, 963
Hpy166II GTNNAC 2 cut(s) 224, 296
Hpy188I TCNGA 2 cut(s) 462, 801
Hpy188III TCNNGA 5 cut(s) 35, 233, 305, 399, 641
Hpy8I GTNNAC 2 cut(s) 224, 296
HpyAV CCTTC 3 cut(s) 165, 504, 881
HpyCH4III ACNGT 9 cut(s) 49, 104, 110, 293, 352, 371, 431, 454, 842
HpyCH4IV ACGT 3 cut(s) 138, 645, 894
HpyCH4V TGCA 6 cut(s) 330, 504, 636, 654, 753, 770
HpyF10VI GCNNNNNNNGC 2 cut(s) 420, 830
HpyF3I CTNAG 1 cut(s) 774
HpySE526I ACGT 3 cut(s) 138, 645, 894
Hsp92I GRCGYC 2 cut(s) 39, 645
Hsp92II CATG 3 cut(s) 46, 118, 504
KroI GCCGGC 1 cut(s) 194
KroNI GCCGGC 1 cut(s) 196
Kzo9I GATC 5 cut(s) 81, 179, 378, 457, 926
LmnI GCTCC 1 cut(s) 956
Lsp1109I GCAGC 2 cut(s) 342, 782
LweI GCATC 1 cut(s) 546
MaeI CTAG 2 cut(s) 266, 689
MaeII ACGT 3 cut(s) 138, 645, 894
MaeIII GTNAC 6 cut(s) 104, 119, 134, 268, 346, 969
MalI GATC 5 cut(s) 83, 181, 380, 459, 928
MbiI CCGCTC 1 cut(s) 797
MboI GATC 5 cut(s) 81, 179, 378, 457, 926
MboII GAAGA 9 cut(s) 83, 86, 174, 235, 268, 539, 640, 683, 737
MflI RGATCY 1 cut(s) 179
MhlI GDGCHC 1 cut(s) 582
MluCI AATT 3 cut(s) 148, 174, 251
MlyI GAGTC 2 cut(s) 238, 302
MmeI TCCRAC 2 cut(s) 469, 499
MnlI CCTC 6 cut(s) 153, 229, 250, 523, 623, 835
MroNI GCCGGC 1 cut(s) 194
MroXI GAANNNNTTC 1 cut(s) 431
MseI TTAA 1 cut(s) 357
MslI CAYNNNNRTG 2 cut(s) 141, 266
MspA1I CMGCKG 2 cut(s) 333, 773
MspI CCGG 4 cut(s) 195, 575, 760, 871
MspR9I CCNGG 3 cut(s) 575, 576, 922
Mva1269I GAATGC 1 cut(s) 636
MvaI CCWGG 1 cut(s) 922
MvnI CGCG 1 cut(s) 480
MwoI GCNNNNNNNGC 2 cut(s) 420, 830
NaeI GCCGGC 1 cut(s) 196
NciI CCSGG 2 cut(s) 575, 576
NcoI CCATGG 1 cut(s) 114
NdeII GATC 5 cut(s) 81, 179, 378, 457, 926
NgoMIV GCCGGC 1 cut(s) 194
NlaIII CATG 3 cut(s) 46, 118, 504
NlaIV GGNNCC 6 cut(s) 112, 164, 415, 534, 748, 958
NmeAIII GCCGAG 1 cut(s) 926
NmuCI GTSAC 5 cut(s) 104, 119, 134, 268, 969
OliI CACNNNNGTG 1 cut(s) 141
PctI GAATGC 1 cut(s) 636
PdiI GCCGGC 1 cut(s) 196
PdmI GAANNNNTTC 1 cut(s) 431
PfoI TCCNGGA 1 cut(s) 920
PinAI ACCGGT 1 cut(s) 759
PkrI GCNGC 4 cut(s) 332, 772, 796, 961
PleI GAGTC 2 cut(s) 237, 302
PpsI GAGTC 2 cut(s) 237, 302
Ppu21I YACGTR 1 cut(s) 139
Psp6I CCWGG 1 cut(s) 920
PspGI CCWGG 1 cut(s) 920
PspN4I GGNNCC 6 cut(s) 112, 164, 415, 534, 748, 958
PspPI GGNCC 3 cut(s) 110, 163, 757
PstI CTGCAG 1 cut(s) 656
PsuI RGATCY 1 cut(s) 179
PvuII CAGCTG 1 cut(s) 773
RsaI GTAC 1 cut(s) 337
RsaNI GTAC 1 cut(s) 336
RseI CAYNNNNRTG 2 cut(s) 141, 266
SaqAI TTAA 1 cut(s) 357
SatI GCNGC 4 cut(s) 331, 771, 795, 960
Sau3AI GATC 5 cut(s) 81, 179, 378, 457, 926
Sau96I GGNCC 3 cut(s) 110, 163, 757
SchI GAGTC 2 cut(s) 238, 302
ScrFI CCNGG 3 cut(s) 575, 576, 922
SduI GDGCHC 1 cut(s) 582
SfaNI GCATC 1 cut(s) 546
SfcI CTRYAG 1 cut(s) 652
SinI GGWCC 3 cut(s) 110, 163, 757
SmaI CCCGGG 1 cut(s) 576
SmiMI CAYNNNNRTG 2 cut(s) 141, 266
Sse9I AATT 3 cut(s) 148, 174, 251
SsiI CCGC 5 cut(s) 90, 333, 795, 845, 960
SspMI CTAG 2 cut(s) 266, 689
StyD4I CCNGG 3 cut(s) 573, 574, 920
StyI CCWWGG 1 cut(s) 114
TaaI ACNGT 9 cut(s) 49, 104, 110, 293, 352, 371, 431, 454, 842
TaiI ACGT 3 cut(s) 141, 648, 897
TaqI TCGA 3 cut(s) 158, 255, 306
TasI AATT 3 cut(s) 148, 174, 251
TauI GCSGC 2 cut(s) 797, 962
Tru1I TTAA 1 cut(s) 357
Tru9I TTAA 1 cut(s) 357
TscAI CASTG 1 cut(s) 298
TseFI GTSAC 5 cut(s) 104, 119, 134, 268, 969
TseI GCWGC 2 cut(s) 330, 770
Tsp45I GTSAC 5 cut(s) 104, 119, 134, 268, 969
TspDTI ATGAA 2 cut(s) 216, 987
TspGWI ACGGA 1 cut(s) 236
TspMI CCCGGG 1 cut(s) 574
TspRI CASTG 1 cut(s) 298
VpaK11BI GGWCC 3 cut(s) 110, 163, 757
XapI RAATTY 2 cut(s) 148, 251
XmaI CCCGGG 1 cut(s) 574
XmnI GAANNNNTTC 1 cut(s) 431
XspI CTAG 2 cut(s) 266, 689
ZraI GACGTC 1 cut(s) 646
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.