FvH4_6g10560

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0009446
Physical Location & Seq
Forward (+)
6328986 .. 6331710
2725 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g10560.t1

Sequence Viewer

Length: 1362 bp
ATGGGAGGTTGCTGCACCAAAGATGCCTTGTATAATGGGAATAACATAGATGATGAGCGTTATTGTAAGGATGATACTGAATTTGACGGTGATGATGATATGAACAATGTGAAGTATGGGGAAGATGGAGCTCGGATAAGAATGCAAGGCTCTTCTAGGTACATTTCAATGTACACCCAACAAGGAAAGAAAGGGATCAATCAAGATGCTATGACAGTTTGGGAGGATTTTGCTGAGAAGGACATGTACTACTGTGGTGTGTTTGATGGCCATGGACCTGATGGTCATAAGATAGCGCGATATGTACGCGACAATTTACCTTCAAAGCTTTCCCAAGTTGTCAAAATCTACCAGCTTAACACCGGCATTTTCGACAATGCTAGTCTTCCTTCAAGTTTTTTCAACCAGGAAAGTAACACAACCCCCATCAGCAAAGGCAGTCAAGATATTTCTCTTTCTTCATGGGAAGCCAGTTTCGTTAAATCATTCAAGGATATGGATGAAGAGCTTAGCCTCGATTCAACCATCGATAGCTTCTGCAGTGGTTCAACAGCTGTAAATGTAGTTAAACAGGGTGATCACTTGGTAATTGCAAACTTGGGAGATTCTCGTGCAGTTCTTGGTACTAGGGGAGAGAATAATCAAATCGTTCCTGTTCAACTCACAGTTGATTTAAAACCGGATACTCCAAGTGAAGCGGAAAGAATTAAGAAGTGTAACGGCAGAATTTTCGCAGTGGATGAAGAACCGGAAGTGTACAGAATATGGATGCCGGATGAAGACTGCCCTGGTCTAGCAATGGCACGAGCATTCGGAGATTTCTGTCTCAAAGATTATGGCCTTATCTCCATTCCTGAAGTTTGCTACAAAACTATCACAAGCGATGATGAATTTGTAGTTTTGGCAACTGATGGGGTATGGGATGCGTTATCGAATGCTAATGTGGTAAAGATAGTTGCTTCTGCAAAGAAGAGATCCAATGCAGCGAAGTTGTTAGTCAAGCGAGCTGTTAGAGCATGGAAAAGAAAATATCCAGAATCCAAAATTGATGATTGTGCAGTTATATGCTTGTTCCTGAAAGACCAACCTTCTTTAAGTCGGTCAATGTCTCATGGAAGCCGGGGAAAAGTAAATGAACCAGCGCTTGTTTCGTATTATAGCACTAAATCGAATGCTCCAACCGATGGAGGAGGCACCGAACTGTCTCTTGTAAATAGCAAGATCTCAGAAGACACAAAAGAAGGGTGGAGTGAATTGGATGGTTCTACAAGAGTGAATTCCCTTTTGAATGTTCCTCGATATGATAACGCTTTGAGTTGGCGGAAAAAATCAAAGGATTTTGAAGAGGTTGAAGCTCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

454

Amino Acids

50.27

Weight (kDa)

4.99

Isoelectric Point (pI)

32.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 83 - 338 6e-38 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 282
AccB1I GGYRCC 1 cut(s) 1193
AccB7I CCANNNNNTGG 1 cut(s) 1184
AccII CGCG 2 cut(s) 298, 309
AciI CCGC 2 cut(s) 698, 1321
AclWI GGATC 2 cut(s) 203, 969
AcoI YGGCCR 1 cut(s) 268
AcsI RAATTY 4 cut(s) 80, 726, 890, 1276
AcuI CTGAAG 1 cut(s) 876
AfaI GTAC 6 cut(s) 161, 173, 248, 306, 625, 758
AfeI AGCGCT 1 cut(s) 1143
AfiI CCNNNNNNNGG 1 cut(s) 1184
AflIII ACRYGT 1 cut(s) 243
AjnI CCWGG 2 cut(s) 405, 787
AluBI AGCT 8 cut(s) 131, 328, 355, 508, 534, 554, 1007, 1355
AluI AGCT 8 cut(s) 131, 328, 355, 508, 534, 554, 1007, 1355
Alw21I GWGCWC 1 cut(s) 133
Alw26I GTCTC 3 cut(s) 830, 1113, 1209
AlwI GGATC 2 cut(s) 203, 969
Aor51HI AGCGCT 1 cut(s) 1143
AoxI GGCC 2 cut(s) 268, 838
ApeKI GCWGC 2 cut(s) 12, 983
ApoI RAATTY 4 cut(s) 80, 726, 890, 1276
AspLEI GCGC 2 cut(s) 298, 1144
AspS9I GGNCC 1 cut(s) 275
AsuC2I CCSGG 1 cut(s) 1121
AsuHPI GGTGA 2 cut(s) 101, 587
AvaII GGWCC 1 cut(s) 275
BalI TGGCCA 1 cut(s) 270
BanI GGYRCC 1 cut(s) 1193
BanII GRGCYC 1 cut(s) 133
BarI GAAGNNNNNNTAC 2 cut(s) 230, 262
BauI CACGAG 2 cut(s) 609, 804
BbsI GAAGAC 3 cut(s) 377, 786, 1236
Bbv12I GWGCWC 1 cut(s) 133
BbvI GCAGC 1 cut(s) 995
BccI CCATC 8 cut(s) 119, 260, 275, 434, 533, 905, 1178, 1253
BceAI ACGGC 1 cut(s) 736
BcgI CGANNNNNNTGC 2 cut(s) 712, 746
BciT130I CCWGG 2 cut(s) 407, 789
BciVI GTATCC 1 cut(s) 676
BclI TGATCA 1 cut(s) 577
BcnI CCSGG 1 cut(s) 1121
BcoDI GTCTC 3 cut(s) 830, 1113, 1209
BfaI CTAG 4 cut(s) 156, 381, 627, 794
BfmI CTRYAG 1 cut(s) 538
BfoI RGCGCY 1 cut(s) 1145
BfuI GTATCC 1 cut(s) 676
BglII AGATCT 1 cut(s) 1221
BisI GCNGC 2 cut(s) 13, 984
BlpI GCTNAGC 1 cut(s) 509
BlsI GCNGC 2 cut(s) 14, 985
Bme1390I CCNGG 3 cut(s) 407, 789, 1121
Bme18I GGWCC 1 cut(s) 275
BmgT120I GGNCC 1 cut(s) 275
BmiI GGNNCC 1 cut(s) 1195
BmrFI CCNGG 3 cut(s) 407, 789, 1121
BmsI GCATC 4 cut(s) 13, 196, 759, 913
BpiI GAAGAC 3 cut(s) 377, 786, 1236
Bpu1102I GCTNAGC 1 cut(s) 509
BpuMI CCSGG 1 cut(s) 1121
Bsa29I ATCGAT 1 cut(s) 528
BsaJI CCNNGG 3 cut(s) 271, 787, 1120
BsaWI WCCGGW 2 cut(s) 679, 748
Bsc4I CCNNNNNNNGG 1 cut(s) 1184
Bse118I RCCGGY 1 cut(s) 362
Bse1I ACTGG 1 cut(s) 471
Bse3DI GCAATG 1 cut(s) 804
BseBI CCWGG 2 cut(s) 407, 789
BseCI ATCGAT 1 cut(s) 528
BseDI CCNNGG 3 cut(s) 271, 787, 1120
BseGI GGATG 7 cut(s) 76, 505, 745, 774, 781, 928, 1264
BseLI CCNNNNNNNGG 1 cut(s) 1184
BseMI GCAATG 1 cut(s) 804
BseMII CTCAG 2 cut(s) 225, 1239
BseNI ACTGG 1 cut(s) 471
BseRI GAGGAG 1 cut(s) 1203
BseXI GCAGC 1 cut(s) 995
BsgI GTGCAG 2 cut(s) 633, 1077
Bsh1236I CGCG 2 cut(s) 298, 309
BshFI GGCC 2 cut(s) 270, 840
BshNI GGYRCC 1 cut(s) 1193
BshVI ATCGAT 1 cut(s) 528
BsiHKAI GWGCWC 1 cut(s) 133
BsiSI CCGG 5 cut(s) 363, 680, 749, 773, 1120
BslI CCNNNNNNNGG 1 cut(s) 1184
BsmAI GTCTC 3 cut(s) 830, 1113, 1209
BsmI GAATGC 4 cut(s) 147, 809, 940, 1177
BsnI GGCC 2 cut(s) 270, 840
Bsp1286I GDGCHC 1 cut(s) 133
Bsp1407I TGTACA 2 cut(s) 171, 756
Bsp143I GATC 4 cut(s) 195, 577, 974, 1221
Bsp1720I GCTNAGC 1 cut(s) 509
Bsp19I CCATGG 1 cut(s) 271
BspACI CCGC 2 cut(s) 698, 1321
BspANI GGCC 2 cut(s) 270, 840
BspCNI CTCAG 2 cut(s) 226, 1238
BspDI ATCGAT 1 cut(s) 528
BspFNI CGCG 2 cut(s) 298, 309
BspLI GGNNCC 1 cut(s) 1195
BspMAI CTGCAG 1 cut(s) 542
BspPI GGATC 2 cut(s) 203, 969
BspQI GCTCTTC 2 cut(s) 157, 498
BspT107I GGYRCC 1 cut(s) 1193
BsrDI GCAATG 1 cut(s) 804
BsrFI RCCGGY 1 cut(s) 362
BsrGI TGTACA 2 cut(s) 171, 756
BsrI ACTGG 1 cut(s) 471
BssAI RCCGGY 1 cut(s) 362
BssECI CCNNGG 3 cut(s) 271, 787, 1120
BssMI GATC 4 cut(s) 195, 577, 974, 1221
BssSI CACGAG 2 cut(s) 609, 804
BssT1I CCWWGG 1 cut(s) 271
Bst2BI CACGAG 2 cut(s) 609, 804
Bst2UI CCWGG 2 cut(s) 407, 789
Bst4CI ACNGT 5 cut(s) 89, 217, 254, 667, 1203
Bst6I CTCTTC 4 cut(s) 157, 498, 965, 1338
BstAUI TGTACA 2 cut(s) 171, 756
BstC8I GCNNGC 1 cut(s) 1005
BstDEI CTNAG 3 cut(s) 234, 509, 1225
BstDSI CCRYGG 1 cut(s) 271
BstF5I GGATG 7 cut(s) 76, 505, 745, 774, 781, 928, 1264
BstFNI CGCG 2 cut(s) 298, 309
BstH2I RGCGCY 1 cut(s) 1145
BstHHI GCGC 2 cut(s) 298, 1144
BstKTI GATC 4 cut(s) 198, 580, 977, 1224
BstMAI GTCTC 3 cut(s) 830, 1113, 1209
BstMBI GATC 4 cut(s) 195, 577, 974, 1221
BstMWI GCNNNNNNNGC 1 cut(s) 1013
BstNI CCWGG 2 cut(s) 407, 789
BstNSI RCATGY 1 cut(s) 247
BstSCI CCNGG 3 cut(s) 405, 787, 1119
BstSFI CTRYAG 1 cut(s) 538
BstUI CGCG 2 cut(s) 298, 309
BstV1I GCAGC 1 cut(s) 995
BstV2I GAAGAC 3 cut(s) 377, 786, 1236
BstX2I RGATCY 2 cut(s) 974, 1221
BstYI RGATCY 2 cut(s) 974, 1221
Bsu15I ATCGAT 1 cut(s) 528
BsuI GTATCC 1 cut(s) 676
BsuRI GGCC 2 cut(s) 270, 840
BsuTUI ATCGAT 1 cut(s) 528
BtgI CCRYGG 1 cut(s) 271
BtgZI GCGATG 1 cut(s) 897
BtsCI GGATG 7 cut(s) 76, 505, 745, 774, 781, 928, 1264
BtsI GCAGTG 2 cut(s) 547, 741
BtsIMutI CAGTG 2 cut(s) 547, 741
Cac8I GCNNGC 1 cut(s) 1005
CfoI GCGC 2 cut(s) 298, 1144
Cfr10I RCCGGY 1 cut(s) 362
Cfr13I GGNCC 1 cut(s) 275
ClaI ATCGAT 1 cut(s) 528
Csp6I GTAC 6 cut(s) 160, 172, 247, 305, 624, 757
CviAII CATG 5 cut(s) 244, 272, 462, 1017, 1112
CviQI GTAC 6 cut(s) 160, 172, 247, 305, 624, 757
DdeI CTNAG 3 cut(s) 234, 509, 1225
DpnI GATC 4 cut(s) 197, 579, 976, 1223
DpnII GATC 4 cut(s) 195, 577, 974, 1221
DraI TTTAAA 1 cut(s) 675
DrdI GACNNNNNNGTC 1 cut(s) 282
DseDI GACNNNNNNGTC 1 cut(s) 282
EaeI YGGCCR 1 cut(s) 268
Eam1104I CTCTTC 4 cut(s) 157, 498, 965, 1338
EarI CTCTTC 4 cut(s) 157, 498, 965, 1338
EciI GGCGGA 1 cut(s) 1336
Ecl136II GAGCTC 1 cut(s) 131
Eco130I CCWWGG 1 cut(s) 271
Eco24I GRGCYC 1 cut(s) 133
Eco47I GGWCC 1 cut(s) 275
Eco47III AGCGCT 1 cut(s) 1143
Eco53kI GAGCTC 1 cut(s) 131
Eco57I CTGAAG 1 cut(s) 876
EcoICRI GAGCTC 1 cut(s) 131
EcoRI GAATTC 1 cut(s) 1276
EcoRII CCWGG 2 cut(s) 405, 787
EcoT14I CCWWGG 1 cut(s) 271
EcoT38I GRGCYC 1 cut(s) 133
ErhI CCWWGG 1 cut(s) 271
FaeI CATG 5 cut(s) 247, 275, 465, 1020, 1115
FatI CATG 5 cut(s) 243, 271, 461, 1016, 1111
FbaI TGATCA 1 cut(s) 577
Fnu4HI GCNGC 2 cut(s) 13, 984
FokI GGATG 7 cut(s) 83, 512, 752, 781, 788, 935, 1271
FriOI GRGCYC 1 cut(s) 133
Fsp4HI GCNGC 2 cut(s) 13, 984
FspBI CTAG 4 cut(s) 156, 381, 627, 794
GlaI GCGC 2 cut(s) 297, 1143
GluI GCNGC 2 cut(s) 13, 984
HaeII RGCGCY 1 cut(s) 1145
HaeIII GGCC 2 cut(s) 270, 840
HapII CCGG 5 cut(s) 363, 680, 749, 773, 1120
HhaI GCGC 2 cut(s) 298, 1144
Hin1II CATG 5 cut(s) 247, 275, 465, 1020, 1115
Hin6I GCGC 2 cut(s) 296, 1142
HinP1I GCGC 2 cut(s) 296, 1142
HindIII AAGCTT 1 cut(s) 326
HinfI GANTC 3 cut(s) 518, 605, 1037
HpaII CCGG 5 cut(s) 363, 680, 749, 773, 1120
HphI GGTGA 2 cut(s) 101, 587
Hpy166II GTNNAC 2 cut(s) 174, 757
Hpy188I TCNGA 3 cut(s) 135, 815, 1228
Hpy188III TCNNGA 5 cut(s) 203, 443, 854, 1034, 1075
Hpy8I GTNNAC 2 cut(s) 174, 757
HpyAV CCTTC 5 cut(s) 232, 330, 399, 1098, 1235
HpyCH4III ACNGT 5 cut(s) 89, 217, 254, 667, 1203
HpyCH4V TGCA 8 cut(s) 15, 145, 540, 593, 614, 965, 983, 1058
HpyF10VI GCNNNNNNNGC 1 cut(s) 1013
HpyF3I CTNAG 3 cut(s) 234, 509, 1225
Hsp92II CATG 5 cut(s) 247, 275, 465, 1020, 1115
HspAI GCGC 2 cut(s) 296, 1142
Ksp22I TGATCA 1 cut(s) 577
Kzo9I GATC 4 cut(s) 195, 577, 974, 1221
LguI GCTCTTC 2 cut(s) 157, 498
LmnI GCTCC 2 cut(s) 128, 1180
Lsp1109I GCAGC 1 cut(s) 995
LweI GCATC 4 cut(s) 13, 196, 759, 913
MaeI CTAG 4 cut(s) 156, 381, 627, 794
MaeIII GTNAC 2 cut(s) 413, 716
MalI GATC 4 cut(s) 197, 579, 976, 1223
MboI GATC 4 cut(s) 195, 577, 974, 1221
MflI RGATCY 2 cut(s) 974, 1221
MhlI GDGCHC 1 cut(s) 133
MlsI TGGCCA 1 cut(s) 270
MluCI AATT 9 cut(s) 80, 313, 588, 705, 726, 890, 1044, 1253, 1276
MluNI TGGCCA 1 cut(s) 270
MmeI TCCRAC 1 cut(s) 1202
MnlI CCTC 6 cut(s) 217, 524, 1181, 1184, 1305, 1339
Mox20I TGGCCA 1 cut(s) 270
MscI TGGCCA 1 cut(s) 270
MseI TTAA 7 cut(s) 357, 480, 567, 674, 708, 1094, 1360
MslI CAYNNNNRTG 1 cut(s) 167
Msp20I TGGCCA 1 cut(s) 270
MspA1I CMGCKG 1 cut(s) 554
MspI CCGG 5 cut(s) 363, 680, 749, 773, 1120
MspR9I CCNGG 3 cut(s) 407, 789, 1121
Mva1269I GAATGC 4 cut(s) 147, 809, 940, 1177
MvaI CCWGG 2 cut(s) 407, 789
MvnI CGCG 2 cut(s) 298, 309
MwoI GCNNNNNNNGC 1 cut(s) 1013
NciI CCSGG 1 cut(s) 1121
NcoI CCATGG 1 cut(s) 271
NdeII GATC 4 cut(s) 195, 577, 974, 1221
NlaIII CATG 5 cut(s) 247, 275, 465, 1020, 1115
NlaIV GGNNCC 1 cut(s) 1195
NspI RCATGY 1 cut(s) 247
PciI ACATGT 1 cut(s) 243
PciSI GCTCTTC 2 cut(s) 157, 498
PctI GAATGC 4 cut(s) 147, 809, 940, 1177
PfeI GAWTC 3 cut(s) 518, 605, 1037
PflMI CCANNNNNTGG 1 cut(s) 1184
PkrI GCNGC 2 cut(s) 14, 985
PscI ACATGT 1 cut(s) 243
Psp124BI GAGCTC 1 cut(s) 133
Psp6I CCWGG 2 cut(s) 405, 787
PspGI CCWGG 2 cut(s) 405, 787
PspN4I GGNNCC 1 cut(s) 1195
PspPI GGNCC 1 cut(s) 275
PstI CTGCAG 1 cut(s) 542
PsuI RGATCY 2 cut(s) 974, 1221
PvuII CAGCTG 1 cut(s) 554
RsaI GTAC 6 cut(s) 161, 173, 248, 306, 625, 758
RsaNI GTAC 6 cut(s) 160, 172, 247, 305, 624, 757
RseI CAYNNNNRTG 1 cut(s) 167
SacI GAGCTC 1 cut(s) 133
SapI GCTCTTC 2 cut(s) 157, 498
SaqAI TTAA 7 cut(s) 357, 480, 567, 674, 708, 1094, 1360
SatI GCNGC 2 cut(s) 13, 984
Sau3AI GATC 4 cut(s) 195, 577, 974, 1221
Sau96I GGNCC 1 cut(s) 275
ScrFI CCNGG 3 cut(s) 407, 789, 1121
SduI GDGCHC 1 cut(s) 133
SfaNI GCATC 4 cut(s) 13, 196, 759, 913
SfcI CTRYAG 1 cut(s) 538
SinI GGWCC 1 cut(s) 275
SmiMI CAYNNNNRTG 1 cut(s) 167
Sse9I AATT 9 cut(s) 80, 313, 588, 705, 726, 890, 1044, 1253, 1276
SsiI CCGC 2 cut(s) 698, 1321
SspMI CTAG 4 cut(s) 156, 381, 627, 794
SstI GAGCTC 1 cut(s) 133
StyD4I CCNGG 3 cut(s) 405, 787, 1119
StyI CCWWGG 1 cut(s) 271
TaaI ACNGT 5 cut(s) 89, 217, 254, 667, 1203
TaqI TCGA 6 cut(s) 372, 516, 528, 932, 1169, 1297
TaqII GACCGA 1 cut(s) 1089
TasI AATT 9 cut(s) 80, 313, 588, 705, 726, 890, 1044, 1253, 1276
TatI WGTACW 3 cut(s) 171, 246, 756
TfiI GAWTC 3 cut(s) 518, 605, 1037
Tru1I TTAA 7 cut(s) 357, 480, 567, 674, 708, 1094, 1360
Tru9I TTAA 7 cut(s) 357, 480, 567, 674, 708, 1094, 1360
TscAI CASTG 2 cut(s) 547, 741
TseI GCWGC 2 cut(s) 12, 983
TspDTI ATGAA 7 cut(s) 116, 450, 516, 756, 792, 903, 1149
TspRI CASTG 2 cut(s) 547, 741
Van91I CCANNNNNTGG 1 cut(s) 1184
VpaK11BI GGWCC 1 cut(s) 275
XapI RAATTY 4 cut(s) 80, 726, 890, 1276
XceI RCATGY 1 cut(s) 247
XcmI CCANNNNNNNNNTGG 1 cut(s) 278
XspI CTAG 4 cut(s) 156, 381, 627, 794
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.