MD04G1167100.v1.1

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0009446
Physical Location & Seq
Reverse (-)
25742293 .. 25744870
2578 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1167100.v1.1.491

Sequence Viewer

Length: 1416 bp
ATGGGAGCTTGTTGTACCAAGGAGAGATCGTATGGAGGAGGGTACATGGAGGATGATGGTGTGGCCCATCGGAATGAGTATTACGAGGCTGAGGAGGAGGAGGAGGATAATGTAAGGTGTGGGGACGATGGGGCCCGCATAAGGTTGCAAGGGTGTTCTAGGAACATTTCCATGTACACCCAGCAAGGAAGAAAAGGGATCAATCAAGATGCCATGACAGTTTGGGAGGACTTTACTGAGAAAGGCATGTACTTCTGCGGTGTGTTTGATGGTCATGGCCCCGAAGGGCACAAAGTTGCAAGATGCGTACGTGACAATTTACCCTCGAAACTCTCGGAAGTCATCAAAATTTACCAGCTCAACACTGGCATATTCAGCGACATTGATGTTGCCGGAAGCGAGATCAATGCAAACAACGGGAATAATCACGACAGGAAACGCAGTCGTGATATCTCTCTTCCGTCATGGGAAGCCAGTTTTGTCAAATCATTCAAGGAAATGGACGAAGAACTTAGCGTTGACAACACCATTGATAGCTTCTGCAGCGGTTCAACTGCAGTAACTGTAGTTAAACAGGGTGATAATTTGGTGATTGCCAACTTAGGCGATTCTCGTGCGGTTCTTGGAACTAGATCAGGAGAAAAAAACCAAATCGTTCCTGTCCAGCTCACGGTTGATTTGAAACCTGATACTCCAAGTGAAGCTGAGAGAATTATGAAATGCAAAGGCAGGATTTTATCAGTGGATGAAGAACCACAAGTGTATAGATTATGGATGCCGGATGAAGATTGCCCTGGTCTAGCAATGGCGAGGGCTTTCGGGGATTTCTGTGTCAAAGATTATGGCCTGATCTCAATTCCTGAAGTTTTCTACAGGAAGATATCAAGCGATGACGAATTTGTGGTCTTGGCAACTGATGGGGTGTGGGATGCTTTAACAAACACTGACGTGGTAAAGATAGTTGCTTCAGCAAGGAAGCGATCCATGGCAGCAGAATTACTAGTAAGGCGTGCGGTTAGAGCATGGAAACGAAAGTTCCCAGCATCAAAAATTGATGACTGCGCAGCTATATGCTTGTATCTGAAAGACAAACCAGCTTCTTCATTAACACATTCCACATCCCAAGTGAGCCGTCTGGGCAAAGTGAATGACTCTGAGCTTTCACTTTCCTATTATAGCACAAGATCGAACATTGTAAGTGATCAGGGAGGCACTGCATCCGAGATTAACAGCAAGATGACGGAGGACTGGAAAGATACTGTGTTGAATTGTAATGTCTCACAAGATGCAAAGGAAGAGTTGAACGCCCTTAAAGAAGTTGAAAGAGTAAATACCATGTTGAAGCTTCCTCGATTTTCTAATGGTTTGAGTCGGAGGAAAACATCAAGGGATTACGAAGAGGTTCAAGCTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

472

Amino Acids

52.55

Weight (kDa)

5.21

Isoelectric Point (pI)

42.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 131 - 340 3.8e-38 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1063
AciI CCGC 5 cut(s) 136, 258, 546, 617, 1013
AclWI GGATC 2 cut(s) 206, 975
AcsI RAATTY 2 cut(s) 348, 896
AcuI CTGAAG 2 cut(s) 882, 951
AfaI GTAC 5 cut(s) 16, 44, 176, 251, 309
AfiI CCNNNNNNNGG 2 cut(s) 141, 670
AgsI TTSAA 8 cut(s) 493, 552, 682, 1267, 1303, 1322, 1342, 1406
AhlI ACTAGT 1 cut(s) 1000
AjiI CACGTC 1 cut(s) 949
AjnI CCWGG 1 cut(s) 793
AleI CACNNNNGTG 1 cut(s) 947
Alw26I GTCTC 1 cut(s) 1282
AlwI GGATC 2 cut(s) 206, 975
AlwNI CAGNNNCTG 1 cut(s) 563
AoxI GGCC 4 cut(s) 63, 132, 277, 844
ApaI GGGCCC 1 cut(s) 136
ApeKI GCWGC 3 cut(s) 543, 989, 1064
ApoI RAATTY 2 cut(s) 348, 896
ArsI GACNNNNNNTTYG 2 cut(s) 888, 920
Asp700I GAANNNNTTC 1 cut(s) 1401
AspLEI GCGC 1 cut(s) 1064
AspS9I GGNCC 4 cut(s) 64, 132, 133, 278
AsuHPI GGTGA 2 cut(s) 590, 601
BaeGI GKGCMC 2 cut(s) 136, 291
BanII GRGCYC 1 cut(s) 136
BauI CACGAG 1 cut(s) 612
BbvCI CCTCAGC 1 cut(s) 90
BbvI GCAGC 3 cut(s) 555, 1001, 1076
BccI CCATC 5 cut(s) 50, 75, 122, 263, 911
BceAI ACGGC 1 cut(s) 1116
BcgI CGANNNNNNTGC 4 cut(s) 389, 423, 596, 630
BciT130I CCWGG 1 cut(s) 795
BclI TGATCA 1 cut(s) 1201
BcoDI GTCTC 1 cut(s) 1282
BcuI ACTAGT 1 cut(s) 1000
BfaI CTAG 4 cut(s) 159, 630, 800, 1001
BfmI CTRYAG 4 cut(s) 541, 555, 564, 871
BglI GCCNNNNNGGC 1 cut(s) 1137
BisI GCNGC 3 cut(s) 544, 990, 1065
BlsI GCNGC 3 cut(s) 545, 991, 1066
Bme1390I CCNGG 1 cut(s) 795
BmgBI CACGTC 1 cut(s) 949
BmgT120I GGNCC 4 cut(s) 64, 132, 133, 278
BmiI GGNNCC 3 cut(s) 133, 134, 280
BmrFI CCNGG 1 cut(s) 795
BmsI GCATC 7 cut(s) 199, 293, 765, 919, 1052, 1226, 1276
Bpu10I CCTNAGC 1 cut(s) 90
BsaAI YACGTR 1 cut(s) 311
BsaJI CCNNGG 3 cut(s) 18, 793, 984
BsaXI ACNNNNNCTCC 2 cut(s) 14, 44
Bsc4I CCNNNNNNNGG 2 cut(s) 141, 670
Bse1I ACTGG 3 cut(s) 370, 474, 1253
Bse3DI GCAATG 1 cut(s) 810
BseBI CCWGG 1 cut(s) 795
BseDI CCNNGG 3 cut(s) 18, 793, 984
BseGI GGATG 7 cut(s) 58, 751, 780, 787, 934, 1118, 1217
BseLI CCNNNNNNNGG 2 cut(s) 141, 670
BseMI GCAATG 1 cut(s) 810
BseMII CTCAG 4 cut(s) 81, 228, 696, 1146
BseNI ACTGG 3 cut(s) 370, 474, 1253
BseRI GAGGAG 5 cut(s) 51, 107, 110, 113, 116
BseSI GKGCMC 2 cut(s) 136, 291
BseXI GCAGC 3 cut(s) 555, 1001, 1076
BseYI CCCAGC 2 cut(s) 180, 1039
BshFI GGCC 4 cut(s) 65, 134, 279, 846
BsiSI CCGG 2 cut(s) 393, 779
BsiWI CGTACG 1 cut(s) 307
BslFI GGGAC 1 cut(s) 137
BslI CCNNNNNNNGG 2 cut(s) 141, 670
BsmAI GTCTC 1 cut(s) 1282
BsmFI GGGAC 1 cut(s) 137
BsnI GGCC 4 cut(s) 65, 134, 279, 846
Bsp120I GGGCCC 1 cut(s) 132
Bsp1286I GDGCHC 2 cut(s) 136, 291
Bsp1407I TGTACA 1 cut(s) 174
Bsp143I GATC 8 cut(s) 26, 198, 402, 632, 849, 980, 1184, 1201
Bsp19I CCATGG 1 cut(s) 984
BspACI CCGC 5 cut(s) 136, 258, 546, 617, 1013
BspANI GGCC 4 cut(s) 65, 134, 279, 846
BspCNI CTCAG 4 cut(s) 82, 229, 697, 1147
BspLI GGNNCC 3 cut(s) 133, 134, 280
BspMAI CTGCAG 2 cut(s) 545, 559
BspPI GGATC 2 cut(s) 206, 975
BsrDI GCAATG 1 cut(s) 810
BsrGI TGTACA 1 cut(s) 174
BsrI ACTGG 3 cut(s) 370, 474, 1253
BssECI CCNNGG 3 cut(s) 18, 793, 984
BssMI GATC 8 cut(s) 26, 198, 402, 632, 849, 980, 1184, 1201
BssSI CACGAG 1 cut(s) 612
BssT1I CCWWGG 2 cut(s) 18, 984
Bst2BI CACGAG 1 cut(s) 612
Bst2UI CCWGG 1 cut(s) 795
Bst4CI ACNGT 4 cut(s) 220, 565, 673, 1261
Bst6I CTCTTC 3 cut(s) 462, 1290, 1392
BstAUI TGTACA 1 cut(s) 174
BstBAI YACGTR 1 cut(s) 311
BstC8I GCNNGC 2 cut(s) 136, 1011
BstDEI CTNAG 6 cut(s) 90, 237, 512, 601, 705, 1155
BstDSI CCRYGG 1 cut(s) 984
BstF5I GGATG 7 cut(s) 58, 751, 780, 787, 934, 1118, 1217
BstHHI GCGC 1 cut(s) 1064
BstKTI GATC 8 cut(s) 29, 201, 405, 635, 852, 983, 1187, 1204
BstMAI GTCTC 1 cut(s) 1282
BstMBI GATC 8 cut(s) 26, 198, 402, 632, 849, 980, 1184, 1201
BstMWI GCNNNNNNNGC 4 cut(s) 375, 543, 1019, 1137
BstNI CCWGG 1 cut(s) 795
BstNSI RCATGY 1 cut(s) 250
BstSCI CCNGG 1 cut(s) 793
BstSFI CTRYAG 4 cut(s) 541, 555, 564, 871
BstSLI GKGCMC 2 cut(s) 136, 291
BstV1I GCAGC 3 cut(s) 555, 1001, 1076
BsuRI GGCC 4 cut(s) 65, 134, 279, 846
BtgI CCRYGG 1 cut(s) 984
BtgZI GCGATG 1 cut(s) 903
BtrI CACGTC 1 cut(s) 949
BtsCI GGATG 7 cut(s) 58, 751, 780, 787, 934, 1118, 1217
BtsI GCAGTG 1 cut(s) 1212
BtsIMutI CAGTG 4 cut(s) 363, 747, 942, 1212
Cac8I GCNNGC 2 cut(s) 136, 1011
CaiI CAGNNNCTG 1 cut(s) 563
CfoI GCGC 1 cut(s) 1064
Cfr13I GGNCC 4 cut(s) 64, 132, 133, 278
Csp6I GTAC 5 cut(s) 15, 43, 175, 250, 308
CviAII CATG 9 cut(s) 46, 172, 214, 247, 275, 465, 985, 1023, 1336
CviQI GTAC 5 cut(s) 15, 43, 175, 250, 308
DdeI CTNAG 6 cut(s) 90, 237, 512, 601, 705, 1155
DpnI GATC 8 cut(s) 28, 200, 404, 634, 851, 982, 1186, 1203
DpnII GATC 8 cut(s) 26, 198, 402, 632, 849, 980, 1184, 1201
Eam1104I CTCTTC 3 cut(s) 462, 1290, 1392
EarI CTCTTC 3 cut(s) 462, 1290, 1392
Eco130I CCWWGG 2 cut(s) 18, 984
Eco24I GRGCYC 1 cut(s) 136
Eco32I GATATC 2 cut(s) 451, 882
Eco57I CTGAAG 2 cut(s) 882, 951
EcoO109I RGGNCCY 1 cut(s) 132
EcoRII CCWGG 1 cut(s) 793
EcoRV GATATC 2 cut(s) 451, 882
EcoT14I CCWWGG 2 cut(s) 18, 984
EcoT38I GRGCYC 1 cut(s) 136
ErhI CCWWGG 2 cut(s) 18, 984
FaeI CATG 9 cut(s) 49, 175, 217, 250, 278, 468, 988, 1026, 1339
FaqI GGGAC 1 cut(s) 137
FatI CATG 9 cut(s) 45, 171, 213, 246, 274, 464, 984, 1022, 1335
FauI CCCGC 1 cut(s) 143
FbaI TGATCA 1 cut(s) 1201
Fnu4HI GCNGC 3 cut(s) 544, 990, 1065
FokI GGATG 7 cut(s) 65, 758, 787, 794, 941, 1105, 1204
FriOI GRGCYC 1 cut(s) 136
Fsp4HI GCNGC 3 cut(s) 544, 990, 1065
FspBI CTAG 4 cut(s) 159, 630, 800, 1001
FspI TGCGCA 1 cut(s) 1063
GlaI GCGC 1 cut(s) 1063
GluI GCNGC 3 cut(s) 544, 990, 1065
GsaI CCCAGC 2 cut(s) 184, 1043
HaeIII GGCC 4 cut(s) 65, 134, 279, 846
HapII CCGG 2 cut(s) 393, 779
HhaI GCGC 1 cut(s) 1064
Hin1II CATG 9 cut(s) 49, 175, 217, 250, 278, 468, 988, 1026, 1339
Hin6I GCGC 1 cut(s) 1062
HinP1I GCGC 1 cut(s) 1062
HincII GTYRAC 1 cut(s) 520
HindII GTYRAC 1 cut(s) 520
HindIII AAGCTT 1 cut(s) 1343
HinfI GANTC 3 cut(s) 608, 1151, 1369
HpaII CCGG 2 cut(s) 393, 779
HphI GGTGA 2 cut(s) 590, 601
Hpy166II GTNNAC 2 cut(s) 177, 520
Hpy188I TCNGA 6 cut(s) 72, 337, 1083, 1156, 1222, 1374
Hpy188III TCNNGA 5 cut(s) 206, 428, 446, 636, 860
Hpy8I GTNNAC 2 cut(s) 177, 520
HpyAV CCTTC 1 cut(s) 278
HpyCH4III ACNGT 4 cut(s) 220, 565, 673, 1261
HpyCH4IV ACGT 2 cut(s) 310, 948
HpyCH4V TGCA 8 cut(s) 148, 299, 410, 543, 557, 723, 1217, 1289
HpyF10VI GCNNNNNNNGC 4 cut(s) 375, 543, 1019, 1137
HpyF3I CTNAG 6 cut(s) 90, 237, 512, 601, 705, 1155
HpySE526I ACGT 2 cut(s) 310, 948
Hsp92II CATG 9 cut(s) 49, 175, 217, 250, 278, 468, 988, 1026, 1339
HspAI GCGC 1 cut(s) 1062
Ksp22I TGATCA 1 cut(s) 1201
Kzo9I GATC 8 cut(s) 26, 198, 402, 632, 849, 980, 1184, 1201
LmnI GCTCC 1 cut(s) 5
Lsp1109I GCAGC 3 cut(s) 555, 1001, 1076
LweI GCATC 7 cut(s) 199, 293, 765, 919, 1052, 1226, 1276
MaeI CTAG 4 cut(s) 159, 630, 800, 1001
MaeII ACGT 2 cut(s) 310, 948
MaeIII GTNAC 2 cut(s) 311, 559
MalI GATC 8 cut(s) 28, 200, 404, 634, 851, 982, 1186, 1203
MboI GATC 8 cut(s) 26, 198, 402, 632, 849, 980, 1184, 1201
MboII GAAGA 9 cut(s) 201, 449, 518, 761, 797, 889, 1092, 1307, 1409
MhlI GDGCHC 2 cut(s) 136, 291
MluCI AATT 9 cut(s) 316, 348, 583, 711, 855, 896, 995, 1050, 1267
MlyI GAGTC 2 cut(s) 1145, 1378
MmeI TCCRAC 1 cut(s) 1352
MroXI GAANNNNTTC 1 cut(s) 1401
MseI TTAA 5 cut(s) 570, 935, 1106, 1227, 1311
MslI CAYNNNNRTG 3 cut(s) 72, 170, 947
MspA1I CMGCKG 1 cut(s) 546
MspI CCGG 2 cut(s) 393, 779
MspR9I CCNGG 1 cut(s) 795
MvaI CCWGG 1 cut(s) 795
MwoI GCNNNNNNNGC 4 cut(s) 375, 543, 1019, 1137
NcoI CCATGG 1 cut(s) 984
NdeII GATC 8 cut(s) 26, 198, 402, 632, 849, 980, 1184, 1201
NlaIII CATG 9 cut(s) 49, 175, 217, 250, 278, 468, 988, 1026, 1339
NlaIV GGNNCC 3 cut(s) 133, 134, 280
NmuCI GTSAC 1 cut(s) 311
NsbI TGCGCA 1 cut(s) 1063
NspI RCATGY 1 cut(s) 250
OliI CACNNNNGTG 1 cut(s) 947
PdmI GAANNNNTTC 1 cut(s) 1401
PfeI GAWTC 1 cut(s) 608
Pfl23II CGTACG 1 cut(s) 307
PkrI GCNGC 3 cut(s) 545, 991, 1066
PleI GAGTC 2 cut(s) 1145, 1377
PpsI GAGTC 2 cut(s) 1145, 1377
Ppu21I YACGTR 1 cut(s) 311
Psp6I CCWGG 1 cut(s) 793
PspFI CCCAGC 2 cut(s) 180, 1039
PspGI CCWGG 1 cut(s) 793
PspLI CGTACG 1 cut(s) 307
PspN4I GGNNCC 3 cut(s) 133, 134, 280
PspOMI GGGCCC 1 cut(s) 132
PspPI GGNCC 4 cut(s) 64, 132, 133, 278
PstI CTGCAG 2 cut(s) 545, 559
PstNI CAGNNNCTG 1 cut(s) 563
RsaI GTAC 5 cut(s) 16, 44, 176, 251, 309
RsaNI GTAC 5 cut(s) 15, 43, 175, 250, 308
RseI CAYNNNNRTG 3 cut(s) 72, 170, 947
SaqAI TTAA 5 cut(s) 570, 935, 1106, 1227, 1311
SatI GCNGC 3 cut(s) 544, 990, 1065
Sau3AI GATC 8 cut(s) 26, 198, 402, 632, 849, 980, 1184, 1201
Sau96I GGNCC 4 cut(s) 64, 132, 133, 278
SchI GAGTC 2 cut(s) 1145, 1378
ScrFI CCNGG 1 cut(s) 795
SduI GDGCHC 2 cut(s) 136, 291
SfaNI GCATC 7 cut(s) 199, 293, 765, 919, 1052, 1226, 1276
SfcI CTRYAG 4 cut(s) 541, 555, 564, 871
SmiMI CAYNNNNRTG 3 cut(s) 72, 170, 947
SpeI ACTAGT 1 cut(s) 1000
Sse9I AATT 9 cut(s) 316, 348, 583, 711, 855, 896, 995, 1050, 1267
SsiI CCGC 5 cut(s) 136, 258, 546, 617, 1013
SspMI CTAG 4 cut(s) 159, 630, 800, 1001
StyD4I CCNGG 1 cut(s) 793
StyI CCWWGG 2 cut(s) 18, 984
TaaI ACNGT 4 cut(s) 220, 565, 673, 1261
TaiI ACGT 2 cut(s) 313, 951
TaqI TCGA 3 cut(s) 326, 1187, 1351
TasI AATT 9 cut(s) 316, 348, 583, 711, 855, 896, 995, 1050, 1267
TatI WGTACW 2 cut(s) 174, 249
TfiI GAWTC 1 cut(s) 608
Tru1I TTAA 5 cut(s) 570, 935, 1106, 1227, 1311
Tru9I TTAA 5 cut(s) 570, 935, 1106, 1227, 1311
TscAI CASTG 4 cut(s) 370, 747, 949, 1219
TseFI GTSAC 1 cut(s) 311
TseI GCWGC 3 cut(s) 543, 989, 1064
Tsp45I GTSAC 1 cut(s) 311
TspDTI ATGAA 4 cut(s) 731, 762, 798, 1092
TspGWI ACGGA 2 cut(s) 450, 1256
TspRI CASTG 4 cut(s) 370, 747, 949, 1219
XapI RAATTY 2 cut(s) 348, 896
XceI RCATGY 1 cut(s) 250
XcmI CCANNNNNNNNNTGG 1 cut(s) 362
XmnI GAANNNNTTC 1 cut(s) 1401
XspI CTAG 4 cut(s) 159, 630, 800, 1001
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.