MD12G1180700.v1.1

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0009446
Physical Location & Seq
Reverse (-)
26060199 .. 26062971
2773 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1180700.v1.1.491

Sequence Viewer

Length: 1413 bp
ATGGGAGCTTGTTGTAGCAAGGAGAAATTGCATGGAGGAGGTTACATGGAGGATGATCATATGGCCAATCGGAATGAGTATTCCGAGGCCGATAAGGAAGAGGATAGTGTAAGGTGTGGGGACGATGGGGCACGCATAAGGTTGGAAGGGTGTTCTAGGAACATATCCATGTACACCCAGCAAGGAAGAAAAGGGATCAATCAAGATGCAATGACAGTTTGGGAGGACTTTACCGAGAAAGGCATGCACTTCTGCGGTGTGTTTGATGGTCATGGCCCCGACGGTCACAAAGTTGCAAGATGTGTACGTGACAATTTACCCTCAAAGCTCTCCGAAGTCATCAAAATTTACCAGCTCAACACCGGCATATTCAGCGACATCAATGTTGCCGGAAGCGAGCTCAATGCAAACAACGGTACAAGTCGCGATAGGAAAGCCAGTAGCGATCTCTCTCTTCCGTCATGGGAAGCCAGTTTCGTTAAATCATTCAAGGAAATGGATGAAGAACTTAGCCTCGATAACACCATTGATAGCTTCTGCAGTGGTTCAACAGCTGTAACTGTACTTAAACAGGGTGATCATTTGGTGATTGCCAACTTAGGCGATTCTCGTGCAGTTCTTGGAACTAGATCAGGAGAGAAAAAACAAATCGTTCCTGTCCAGCTCACAGTTGATTTGAAACCTGATACTCCAAGTGAAGCTGAGAGAATTATGAAATGCAAGGGCAGGATTTTATCAGTGGAGGGAGAACCACAAGTATTTAGATTATGGATGCCGGATGAAGACTGCCCTGGTCTTGCAATGGCAAGGGCTTTCGGGGATTTCTGTGTCAAAGATTACGGTCTAACCTCAAGTCCGGAAGTTTTCTACAGGAAGATATCAAGTGATGATGAATTTGTGGTCTTGGCAACTGATGGGATTTGGGATGCTTTAACAAACAGTGACGTGGTAAAGATAGTTGCATCAGCAAGGAAGCGATCCATGGCAGCAGAATTACTAGTAAAGCGTGCAGTTAGAGCATGGAAACGGAAGTTCCCCCAATCGAAAATTGATGATTGTGCAGTTATATGCTTGTACCTGAAGGACCAACCTGCTTCTTCATTAACACAATCCGATTCCCATGCGAGCCGTGTAGGCAAAGGAAATGACTCTGAGCTTTCCCTTTCTTACTATAGCAAAAGATCGAATGTTGCAAGCGATCAGGGAGGCCCTGCATCCGAGATCAACAGCAAGATGATGGAGGATGAGAAGGATACCGTGTTGAACTGCAATGTCTCACAAGACGCAAAGGAAGAGTGGAATGCCATTAAAAGTGTTGACAGAGTAAATACCATGTTGAAGCTTCCTCAATTTTCTAATGGTTTGAGTCGGAGGAAAACATCAAAGGATTACGAAGAGGTTGAAGCTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

471

Amino Acids

51.99

Weight (kDa)

5.36

Isoelectric Point (pI)

40.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 130 - 339 1.4e-40 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1099
AccII CGCG 1 cut(s) 426
AccIII TCCGGA 1 cut(s) 856
AciI CCGC 1 cut(s) 255
AclWI GGATC 2 cut(s) 203, 972
AcoI YGGCCR 1 cut(s) 63
AcsI RAATTY 2 cut(s) 345, 893
AcuI CTGAAG 1 cut(s) 1100
AfaI GTAC 5 cut(s) 173, 306, 418, 564, 1076
AgsI TTSAA 6 cut(s) 490, 549, 679, 1264, 1339, 1403
AhlI ACTAGT 1 cut(s) 997
AjiI CACGTC 1 cut(s) 946
AjnI CCWGG 1 cut(s) 790
Alw21I GWGCWC 1 cut(s) 402
Alw26I GTCTC 1 cut(s) 1279
AlwI GGATC 2 cut(s) 203, 972
Aor13HI TCCGGA 1 cut(s) 856
AoxI GGCC 4 cut(s) 63, 87, 274, 1207
ApeKI GCWGC 1 cut(s) 986
ApoI RAATTY 2 cut(s) 345, 893
AspS9I GGNCC 3 cut(s) 275, 1084, 1208
AsuHPI GGTGA 2 cut(s) 587, 598
AvaII GGWCC 1 cut(s) 1084
BaeGI GKGCMC 1 cut(s) 133
BalI TGGCCA 1 cut(s) 65
BanII GRGCYC 1 cut(s) 402
BauI CACGAG 1 cut(s) 609
BbsI GAAGAC 1 cut(s) 789
Bbv12I GWGCWC 1 cut(s) 402
BbvI GCAGC 1 cut(s) 998
BccI CCATC 4 cut(s) 119, 260, 908, 1231
BceAI ACGGC 1 cut(s) 1113
BcgI CGANNNNNNTGC 6 cut(s) 386, 420, 593, 627, 1103, 1137
BciT130I CCWGG 1 cut(s) 792
BciVI GTATCC 1 cut(s) 1246
BclI TGATCA 2 cut(s) 55, 577
BcoDI GTCTC 1 cut(s) 1279
BcuI ACTAGT 1 cut(s) 997
BfaI CTAG 3 cut(s) 156, 627, 998
BfmI CTRYAG 3 cut(s) 538, 868, 1171
BfuAI ACCTGC 1 cut(s) 1099
BfuI GTATCC 1 cut(s) 1246
BglI GCCNNNNNGGC 1 cut(s) 1134
BisI GCNGC 1 cut(s) 987
BlsI GCNGC 1 cut(s) 988
Bme1390I CCNGG 1 cut(s) 792
Bme18I GGWCC 1 cut(s) 1084
BmgBI CACGTC 1 cut(s) 946
BmgT120I GGNCC 3 cut(s) 275, 1084, 1208
BmiI GGNNCC 1 cut(s) 277
BmrFI CCNGG 1 cut(s) 792
BmsI GCATC 5 cut(s) 196, 762, 916, 971, 1223
BpiI GAAGAC 1 cut(s) 789
BpuEI CTTGAG 1 cut(s) 835
BsaAI YACGTR 1 cut(s) 308
BsaJI CCNNGG 3 cut(s) 84, 790, 981
BsaWI WCCGGW 1 cut(s) 856
Bse118I RCCGGY 1 cut(s) 362
Bse1I ACTGG 2 cut(s) 438, 471
Bse3DI GCAATG 3 cut(s) 216, 807, 1276
BseAI TCCGGA 1 cut(s) 856
BseBI CCWGG 1 cut(s) 792
BseDI CCNNGG 3 cut(s) 84, 790, 981
BseGI GGATG 7 cut(s) 58, 505, 777, 784, 931, 1214, 1249
BseMI GCAATG 3 cut(s) 216, 807, 1276
BseMII CTCAG 2 cut(s) 693, 1143
BseNI ACTGG 2 cut(s) 438, 471
BseRI GAGGAG 1 cut(s) 51
BseSI GKGCMC 1 cut(s) 133
BseXI GCAGC 1 cut(s) 998
BseYI CCCAGC 1 cut(s) 177
BsgI GTGCAG 3 cut(s) 633, 1029, 1080
Bsh1236I CGCG 1 cut(s) 426
BshFI GGCC 4 cut(s) 65, 89, 276, 1209
BsiHKAI GWGCWC 1 cut(s) 402
BsiSI CCGG 4 cut(s) 363, 390, 776, 857
BslFI GGGAC 1 cut(s) 134
BsmAI GTCTC 1 cut(s) 1279
BsmFI GGGAC 1 cut(s) 134
BsmI GAATGC 1 cut(s) 1306
BsnI GGCC 4 cut(s) 65, 89, 276, 1209
Bsp1286I GDGCHC 2 cut(s) 133, 402
Bsp13I TCCGGA 1 cut(s) 856
Bsp1407I TGTACA 1 cut(s) 171
Bsp143I GATC 9 cut(s) 55, 195, 445, 577, 629, 977, 1181, 1198, 1221
Bsp19I CCATGG 1 cut(s) 981
Bsp68I TCGCGA 1 cut(s) 426
BspACI CCGC 1 cut(s) 255
BspANI GGCC 4 cut(s) 65, 89, 276, 1209
BspCNI CTCAG 2 cut(s) 694, 1144
BspEI TCCGGA 1 cut(s) 856
BspFNI CGCG 1 cut(s) 426
BspLI GGNNCC 1 cut(s) 277
BspMAI CTGCAG 1 cut(s) 542
BspMI ACCTGC 1 cut(s) 1099
BspPI GGATC 2 cut(s) 203, 972
BsrDI GCAATG 3 cut(s) 216, 807, 1276
BsrFI RCCGGY 1 cut(s) 362
BsrGI TGTACA 1 cut(s) 171
BsrI ACTGG 2 cut(s) 438, 471
BssAI RCCGGY 1 cut(s) 362
BssECI CCNNGG 3 cut(s) 84, 790, 981
BssMI GATC 9 cut(s) 55, 195, 445, 577, 629, 977, 1181, 1198, 1221
BssSI CACGAG 1 cut(s) 609
BssT1I CCWWGG 1 cut(s) 981
Bst2BI CACGAG 1 cut(s) 609
Bst2UI CCWGG 1 cut(s) 792
Bst4CI ACNGT 8 cut(s) 217, 284, 416, 562, 670, 842, 941, 1258
Bst6I CTCTTC 4 cut(s) 93, 459, 1287, 1389
BstAUI TGTACA 1 cut(s) 171
BstBAI YACGTR 1 cut(s) 308
BstC8I GCNNGC 6 cut(s) 133, 245, 398, 1008, 1126, 1195
BstDEI CTNAG 4 cut(s) 509, 598, 702, 1152
BstDSI CCRYGG 1 cut(s) 981
BstF5I GGATG 7 cut(s) 58, 505, 777, 784, 931, 1214, 1249
BstFNI CGCG 1 cut(s) 426
BstKTI GATC 9 cut(s) 58, 198, 448, 580, 632, 980, 1184, 1201, 1224
BstMAI GTCTC 1 cut(s) 1279
BstMBI GATC 9 cut(s) 55, 195, 445, 577, 629, 977, 1181, 1198, 1221
BstMWI GCNNNNNNNGC 3 cut(s) 372, 1016, 1134
BstNI CCWGG 1 cut(s) 792
BstNSI RCATGY 1 cut(s) 247
BstSCI CCNGG 1 cut(s) 790
BstSFI CTRYAG 3 cut(s) 538, 868, 1171
BstSLI GKGCMC 1 cut(s) 133
BstUI CGCG 1 cut(s) 426
BstV1I GCAGC 1 cut(s) 998
BstV2I GAAGAC 1 cut(s) 789
BsuI GTATCC 1 cut(s) 1246
BsuRI GGCC 4 cut(s) 65, 89, 276, 1209
BtgI CCRYGG 1 cut(s) 981
BtrI CACGTC 1 cut(s) 946
BtsCI GGATG 7 cut(s) 58, 505, 777, 784, 931, 1214, 1249
BtsI GCAGTG 1 cut(s) 547
BtsIMutI CAGTG 3 cut(s) 547, 744, 946
BtuMI TCGCGA 1 cut(s) 426
BveI ACCTGC 1 cut(s) 1099
Cac8I GCNNGC 6 cut(s) 133, 245, 398, 1008, 1126, 1195
Cfr10I RCCGGY 1 cut(s) 362
Cfr13I GGNCC 3 cut(s) 275, 1084, 1208
CseI GACGC 1 cut(s) 1292
Csp6I GTAC 5 cut(s) 172, 305, 417, 563, 1075
CviQI GTAC 5 cut(s) 172, 305, 417, 563, 1075
DdeI CTNAG 4 cut(s) 509, 598, 702, 1152
DpnI GATC 9 cut(s) 57, 197, 447, 579, 631, 979, 1183, 1200, 1223
DpnII GATC 9 cut(s) 55, 195, 445, 577, 629, 977, 1181, 1198, 1221
EaeI YGGCCR 1 cut(s) 63
Eam1104I CTCTTC 4 cut(s) 93, 459, 1287, 1389
EarI CTCTTC 4 cut(s) 93, 459, 1287, 1389
Ecl136II GAGCTC 1 cut(s) 400
Eco130I CCWWGG 1 cut(s) 981
Eco24I GRGCYC 1 cut(s) 402
Eco32I GATATC 1 cut(s) 879
Eco47I GGWCC 1 cut(s) 1084
Eco53kI GAGCTC 1 cut(s) 400
Eco57I CTGAAG 1 cut(s) 1100
EcoICRI GAGCTC 1 cut(s) 400
EcoO109I RGGNCCY 1 cut(s) 1208
EcoRII CCWGG 1 cut(s) 790
EcoRV GATATC 1 cut(s) 879
EcoT14I CCWWGG 1 cut(s) 981
EcoT38I GRGCYC 1 cut(s) 402
ErhI CCWWGG 1 cut(s) 981
FaqI GGGAC 1 cut(s) 134
FauNDI CATATG 1 cut(s) 60
FbaI TGATCA 2 cut(s) 55, 577
Fnu4HI GCNGC 1 cut(s) 987
FokI GGATG 7 cut(s) 65, 512, 784, 791, 938, 1201, 1256
FriOI GRGCYC 1 cut(s) 402
Fsp4HI GCNGC 1 cut(s) 987
FspBI CTAG 3 cut(s) 156, 627, 998
GluI GCNGC 1 cut(s) 987
GsaI CCCAGC 1 cut(s) 181
HaeIII GGCC 4 cut(s) 65, 89, 276, 1209
HapII CCGG 4 cut(s) 363, 390, 776, 857
HgaI GACGC 1 cut(s) 1292
HincII GTYRAC 1 cut(s) 1318
HindII GTYRAC 1 cut(s) 1318
HindIII AAGCTT 1 cut(s) 1340
HinfI GANTC 4 cut(s) 605, 1115, 1148, 1366
HpaII CCGG 4 cut(s) 363, 390, 776, 857
HphI GGTGA 2 cut(s) 587, 598
Hpy166II GTNNAC 3 cut(s) 174, 305, 1318
Hpy188I TCNGA 7 cut(s) 72, 85, 334, 1114, 1153, 1219, 1371
Hpy188III TCNNGA 4 cut(s) 203, 425, 633, 857
Hpy8I GTNNAC 3 cut(s) 174, 305, 1318
Hpy99I CGWCG 1 cut(s) 284
HpyAV CCTTC 3 cut(s) 140, 1075, 1243
HpyCH4III ACNGT 8 cut(s) 217, 284, 416, 562, 670, 842, 941, 1258
HpyCH4IV ACGT 2 cut(s) 307, 945
HpyF10VI GCNNNNNNNGC 3 cut(s) 372, 1016, 1134
HpyF3I CTNAG 4 cut(s) 509, 598, 702, 1152
HpySE526I ACGT 2 cut(s) 307, 945
Kpn2I TCCGGA 1 cut(s) 856
Ksp22I TGATCA 2 cut(s) 55, 577
Kzo9I GATC 9 cut(s) 55, 195, 445, 577, 629, 977, 1181, 1198, 1221
LmnI GCTCC 1 cut(s) 5
Lsp1109I GCAGC 1 cut(s) 998
LweI GCATC 5 cut(s) 196, 762, 916, 971, 1223
MaeI CTAG 3 cut(s) 156, 627, 998
MaeII ACGT 2 cut(s) 307, 945
MaeIII GTNAC 5 cut(s) 41, 284, 308, 556, 941
MalI GATC 9 cut(s) 57, 197, 447, 579, 631, 979, 1183, 1200, 1223
MboI GATC 9 cut(s) 55, 195, 445, 577, 629, 977, 1181, 1198, 1221
MboII GAAGA 9 cut(s) 110, 198, 446, 515, 794, 886, 1089, 1304, 1406
MhlI GDGCHC 2 cut(s) 133, 402
MlsI TGGCCA 1 cut(s) 65
MluCI AATT 8 cut(s) 26, 313, 345, 708, 893, 992, 1047, 1349
MluNI TGGCCA 1 cut(s) 65
MlyI GAGTC 2 cut(s) 1142, 1375
MmeI TCCRAC 2 cut(s) 123, 1349
Mox20I TGGCCA 1 cut(s) 65
MroI TCCGGA 1 cut(s) 856
MscI TGGCCA 1 cut(s) 65
MseI TTAA 5 cut(s) 480, 567, 932, 1103, 1308
MslI CAYNNNNRTG 1 cut(s) 167
Msp20I TGGCCA 1 cut(s) 65
MspA1I CMGCKG 1 cut(s) 554
MspI CCGG 4 cut(s) 363, 390, 776, 857
MspR9I CCNGG 1 cut(s) 792
Mva1269I GAATGC 1 cut(s) 1306
MvaI CCWGG 1 cut(s) 792
MvnI CGCG 1 cut(s) 426
MwoI GCNNNNNNNGC 3 cut(s) 372, 1016, 1134
NcoI CCATGG 1 cut(s) 981
NdeI CATATG 1 cut(s) 60
NdeII GATC 9 cut(s) 55, 195, 445, 577, 629, 977, 1181, 1198, 1221
NlaIV GGNNCC 1 cut(s) 277
NmuCI GTSAC 3 cut(s) 284, 308, 941
NruI TCGCGA 1 cut(s) 426
NspI RCATGY 1 cut(s) 247
PaeI GCATGC 1 cut(s) 247
PctI GAATGC 1 cut(s) 1306
PfeI GAWTC 2 cut(s) 605, 1115
PkrI GCNGC 1 cut(s) 988
PleI GAGTC 2 cut(s) 1142, 1374
PpsI GAGTC 2 cut(s) 1142, 1374
Ppu21I YACGTR 1 cut(s) 308
Psp124BI GAGCTC 1 cut(s) 402
Psp6I CCWGG 1 cut(s) 790
PspFI CCCAGC 1 cut(s) 177
PspGI CCWGG 1 cut(s) 790
PspN4I GGNNCC 1 cut(s) 277
PspPI GGNCC 3 cut(s) 275, 1084, 1208
PstI CTGCAG 1 cut(s) 542
PvuII CAGCTG 1 cut(s) 554
RruI TCGCGA 1 cut(s) 426
RsaI GTAC 5 cut(s) 173, 306, 418, 564, 1076
RsaNI GTAC 5 cut(s) 172, 305, 417, 563, 1075
RseI CAYNNNNRTG 1 cut(s) 167
SacI GAGCTC 1 cut(s) 402
SaqAI TTAA 5 cut(s) 480, 567, 932, 1103, 1308
SatI GCNGC 1 cut(s) 987
Sau3AI GATC 9 cut(s) 55, 195, 445, 577, 629, 977, 1181, 1198, 1221
Sau96I GGNCC 3 cut(s) 275, 1084, 1208
SchI GAGTC 2 cut(s) 1142, 1375
ScrFI CCNGG 1 cut(s) 792
SduI GDGCHC 2 cut(s) 133, 402
SfaNI GCATC 5 cut(s) 196, 762, 916, 971, 1223
SfcI CTRYAG 3 cut(s) 538, 868, 1171
SinI GGWCC 1 cut(s) 1084
SmiMI CAYNNNNRTG 1 cut(s) 167
SmlI CTYRAG 1 cut(s) 850
SmoI CTYRAG 1 cut(s) 850
SpeI ACTAGT 1 cut(s) 997
SphI GCATGC 1 cut(s) 247
Sse9I AATT 8 cut(s) 26, 313, 345, 708, 893, 992, 1047, 1349
SsiI CCGC 1 cut(s) 255
SspMI CTAG 3 cut(s) 156, 627, 998
SstI GAGCTC 1 cut(s) 402
StyD4I CCNGG 1 cut(s) 790
StyI CCWWGG 1 cut(s) 981
TaaI ACNGT 8 cut(s) 217, 284, 416, 562, 670, 842, 941, 1258
TaiI ACGT 2 cut(s) 310, 948
TaqI TCGA 3 cut(s) 516, 1043, 1184
TasI AATT 8 cut(s) 26, 313, 345, 708, 893, 992, 1047, 1349
TatI WGTACW 2 cut(s) 171, 562
TfiI GAWTC 2 cut(s) 605, 1115
Tru1I TTAA 5 cut(s) 480, 567, 932, 1103, 1308
Tru9I TTAA 5 cut(s) 480, 567, 932, 1103, 1308
TscAI CASTG 3 cut(s) 547, 744, 946
TseFI GTSAC 3 cut(s) 284, 308, 941
TseI GCWGC 1 cut(s) 986
Tsp45I GTSAC 3 cut(s) 284, 308, 941
TspDTI ATGAA 5 cut(s) 516, 728, 795, 906, 1089
TspGWI ACGGA 2 cut(s) 447, 1042
TspRI CASTG 3 cut(s) 547, 744, 946
VpaK11BI GGWCC 1 cut(s) 1084
XapI RAATTY 2 cut(s) 345, 893
XceI RCATGY 1 cut(s) 247
XspI CTAG 3 cut(s) 156, 627, 998
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.