AT5G02310

at5g02310 (e 0.0) prt6 prt6 (proteolysis 6)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007182
Physical Location & Seq
Forward (+)
473868 .. 482942
9075 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G02310.2

Sequence Viewer

Length: 6021 bp
ATGGAGACCAACTCTTCTCTTTTTGGTTTAGTTTCTCCTAGCTCGCATGATTTGGTCATTGAGAGGCTTGCTTCTGTGGGGGTTCCTAAGAAATACCGCTCTAAACGTGGCCTAGTGGAATTCGTTAGAGCTAACCCGGCCAAGATTTCAGAGTTAGTCTCTGCTCTCTTACCTACAGATGACGATGTTAAACTAGGATTGAAAGAAGCTAGGGAACGGCCTCGAAAGTCTGCTGTGAGTCCTACCATGAAAAAGAGGTTCAGGGAGAGCATGAATATGCTGCAGTGGTTGATGTTCCAGGATGAGCCTGATGTTTCTTTGAGGAATCTAGCAAAACTGAATCTTGATCAGCGAGGTGTTTGTGGCTCTGTCTGGGGACAGAATGATATAGCATATAGGTGTAGGACATGCGAGAATGACCCAACTTGTGCAATCTGCGTGCCTTGTTTCCAGAATGGGGATCACAACTCCCACGATTATTCAATCATCTACACAGGTGGTGGTTGTTGTGATTGTGGGGATGAAACAGCATGGAAACCTGATGGTTTCTGTTCAAATCATAAAGGTTCTGAACAGATTCGACCCCTCTCAGAAAATCTAGCAAATTCAGTTGGGCCTATACTTGATGCCCTTTTTACTTGTTGGAATAACAAGCTCTTATCTGCAGAAAGTAGTGGTCAGAAAGGTGCTAGATCCAACGATACTCTTGTGATACTCCAAAAGATGTCAAACGAGCTGACATTTATAGTGGTTGAAATGCTTCTGGAGTTTTCTATGTCCAGTGAGAGTTTGCTCAGTTTTGTTTCTAGAAGGATTATCTCTTCAAGTGGTTTATTGAGCATTCTCTTAAAGGCTGAGAGGTTCTTGGACCAAGATGTTATGAAGAAACTACATGACTTGTTCCTCAAATTAATAGGAGATCCAGTCTTCAAGTGTGAATTTGCTAAAGCATTTGTGAGTTATTATCCAGTTGTGATAAGCGAAGTGGTTAAGCAGGGTACTGATAATGCATTCAAGAAATATCCTCTACTGTCCACATTTTCTGTGCAAATCCTCACGGTGCCAACTCTAACACCATTTCTGGTGAAGGAAATGAATTTGCTAGCTATGCTTTTGGGATGCCTCAGTGATATCTTTGTTTCTTGTTCTGGGGAGGATGGTTTGCTACAGGCTACAAAGTTGGAACGGTTGTGTGAGACAAGTGAACGTGTCATTGGAGACTTGAAATTTGTTATGAGCCATGCTATAGTTTCTAAGTATGCAACACATGAACACCGGGAGTTATCAAGATCATGGTTGACACTCTTGACCTTTGCTCAAGGAATGAATCCTTTAAAAAGAGAGACTGGAATCCCTATTGACGAAGAAAATGATTACATGCATCTGTTCTTTGTTTTGGGTCATTCTATAGCTGTTATTCACTCTCTATTGGTTAATGGTACATATTCTGCTGCCAGTGATGAAGAAATAGAAAATGATAGAAACGCCAAGGAAGAATTTGACAAGTGTGATGGAGATGGAGAAAGGTATGCAAAAGTTGGAAGATTGTCTCATGAAGATTCTGTATGTACTGCGATTGTAAGCAGCAGCTCCTTTGATAGCTCAATGGCCTCTGAAGTCCACAAAATTGATCCTTTCCATGCCCTGCTTCCTTCTTCTGCCATATATTTGATACGCGAATGTTTGAAAGTTTTGGAGACATGCTTAGGAAATGATGAAGGTATATCAAAGTTTCTTTGCAAGTTGTCCTCTTCTAGCGGCAGAAACATCCCTGAAAGTAAGATGTCGTGGCCAAGGAGAGATTTGTTAAATGTTGAAACTGGAGGAAGTGTATCTAGTAATCTTGCCAGTTCTAGTAGAGATCCGAGTACTGGCTTATCACCTCTATGCGGCGATATTCAAACAAATCTTAGCTTGGATAATGTTTGTGGACCGTATGGGGTGGTTCAGACAGATGTTACAGCTGACTCTAAAAGAGTATCTTGCAACTCTGCTGATTTGACAAAGAATGCATCAGGATTACGTATACTTGGTCTGTGTGATTGGCCGGACATTCACTATGATGTGAGTTCTCAGGCCATATCAGTTCATCTTCCTTTGCACCGGTTACTTTCTCTGCTAATACAAAAAGCATTAAGGATATGTTATGGAGAATCTGCATCATACAATGGAGTCAGTATTAGTCATGAGATCCCACATGCAGACTTCTTCAGCTCCGTGATAGGAGATTTCCATCCTTGTGGATTTTCTGCACTCGTTATGGAACATGTTCTACAGATTAGGGTGTTTTGTGCCCAGGTTATCGCTGGAATGTGGAAGAAGAATGGGGATTCTGCATTAGTATCTTGTGAGTGGTATCGGTCAGTTCGTTGGTCAGAACAGGGACTTGAGCTTGATCTATTTCTTCTCCAGTGTTGTGCTGCGTTAGCTCCCGCAGATTCTTATGTTGATAAGCTTCTCAGTCGATTTGGGCTCTCAAGCTATCTTTCACTTAATCCGGATATAACAAATGAGTATGAACCAGTTTTGGTCCAGGAAATGCTTGGTCTTCTGATACAGATTCTGCAAGAAAGACGATTTTGTGGTCTTTCTACTGCTGAAAGTTTGAGAAGAGAGATCATCTTCAAGTTAGCCACTGGAGATTTCACTCATAGTCAATTGGTCAAATCTTTACCCCGTGACCTGTCCAAGTCGGACGAGCTCCAGGAGGTCTTAGATGACGTTTCAGTCTATTGCAATCCATCCGGCATGAACCAGGGAAAGTATTCACTACAGTCATCTTGTTGGAAGGAATTGGATCTCTACCACCCTCGTTGGCAGTCGAGAGACTTGCAATCTGCAGAAGAAAGATTTTCGCGTTATTGTGGAGTGTCTGCGTTGACCACTCAGCTTCCGAGGTGGAGAATGATCTATCCACCTCTAAAGGGGCTTGCTAGAATAGGCACTTGCAAAGCCACATTTCAAATAATTTCATCTGCTTTATACTACGCACTACAGAGTGGTACATCAGTTAAATCACGTGCTCCTGATGGTGTTCTCATAACTGCACTGCAGTTACTCTCGTTATCATTGGATATTTGCACTCAGCAGAGGCAATCTAATAGTCAGGATTGTTGCTTAGAAAATTCAATTCCAATTCTCGAATTAGCTGGTCTGGAAATAATTGGTATAGCTCAAGGGACTGAAAAAGAAAGTTTATTGTCTCTTCTTGTTTCGTTAATGAAGACTCGTATGGGAGATGGCCGCCATCAATTTCCAGAGCCAGGCAGTTGCAATATTTCGTCCTGGATTGGAAATCTGCTGAAGAAGTTCAGTGCAATTGATTCTGTTTGTATGAACCTATTGCAAAGTCTTGCCCCCGAGGTGGTTGGTCAATCTGGCTTCGATAAAGTCATGTCAGGTTCTACTTCTGATGAGAAGCGCAAGGCTAAAGCTAAAGAGAGGCAGGCTGCTATTATGGCAAAAATGAAAGCTGAACAGTCAAAGTTCTTGTCTACCTTGAGTTCCAGCATGGATGATGATGATCCGAGATCTGAATTTGAAACAAGTGATTCTGTGATGGAACATGATTCAGAAATAGCTGTCCGTGAAGTTTGCTCCCTCTGCCATGACCCAGATTCCAAAGACCCCGTTTCTTTCTTGATTTTTCTCCAGAAATCTAAACTATTGAGTTTTGTGGATAGAGGCCCTCCATCTTGGGATCAATGTCCACAATCCGAAAAGAAGATATCTGTGGATGGAGCTCCTGATCTTTTGAGAATGAATGCTTCTTCAGATAGTTTAAGAATAAGTTCTCCTCTCATGCTGCAATTGTCAGATGATACTATCTCTGAGTCTGCTAATATGATTGAATCTATAAAAGCCCGGCTTATAGGGAATGGTCAGACAGAGAAGAGGTCTAGCGATGGGAGGGGAAAAGATGAATCTAACATGGAATCATTGGAGATAGCCATGTATCAAACTGTTCGCAACAAAATTGAAAACATGATAAATCAAAGCCTTACACGTGTAGATCATCAACCCCATGAAGCTGAAAATTGTTCGGAGAAAAATTCTGTTGGTGGTCCATCTACTTTGCAAGGTAGATTTCCTGATATTCGATCAAGACAAACTTCCAGGCGTCCTGATGCTGGTTCAGATGGGTTTCACCCTATCGACTGTGATGGGGTTTATCTTTCATCATGTGGACATGCTGTACATCAAAGCTGCCTTGAACGATATTTAAAATCCTTAAAGGAAAGGTCTGGCAGAAGAACTGTCTTTGAAGGTGCACATATTGTGGATCTAAAAAAGAAAGAATTCCTGTGTCCTGTATGCCGCCGACTAGCCAATTCTGTATTGCCTGAATGTCCTGGAGATTTATGTTCTGTCTCAAAGCTACAAGATAGTCCACGTACTAAGTTACGTAGAAAAGATGCACTGCAGCCTTCTCTTTGGCTCTCTGAAGCGTTGTGTCTACTACGATCTGCTGCTGAAGTGATCGAAGATGGTGATAGAGGTAAAACAGTTACTCCACAGGGAGATGGACCAAGAAGGAAAGATCTTAAATCTGTCTCTAAAATGCTCTGGGATTTCTATTTTCCCAAGCCAGAGGATAAAACTCTAAAACGTCTGTGGCTACCACCACAATCAATCGTCATGTGGGACACCCTTAAGTACTCTCTCATTTCAATGGAAATTGGTACTCGTTTTGCCAAGAATTCAATGCTGCCTGTCTATTGTATTGACTCCTTGTATGAAGAGTTGAAAACCTCTAAGGGGACTATTTTGTCAGTTTTATTACGAGTGGTACAGAGCTCCAGAACAAAGAATACTATTCATGTTCGTCAGAGATTTGTGGGTATGAAGCATCTTGCAGAGTCTATATGCTATGGAGTTTCAAGTAGTTCTTCGAGCAGCATATTTGGAAGTGAAGGCACAACAGGTTCATTGAAGAATATAGACCTGCTCTGGAATCGAGCATCCGATCCAGTTCTTGCCCATGACCCATTTTCATCACTGATGTGGGCTCTCTTTTGTCTTCCTTTTCCTTTTCTCACGTGCGAGGAATCTCTATTGTCACTTGTGCATATTTTCCACAGTGTCTCGCTTGTTCAGACTGTAATTGCTTATTGTGCATGTCGCCCAAGTGAATTGAGTGAATTAAATTTTGGGGAAAACCTGCTTAATGACATCTCCAACGCTTTGAGAGAATCAGGTGGTTGGGAGTATTTCAGGTCGAACAATATGGATTTGTCATGTGATATAAAAGACACAATCCGTAAATATAGTCTTCCTTTCTTGAGGAGATGTGCGTTGTTGTGGAAGCTACTGAAATCTACTCCTAGGAAGCTTCATGAAGAGTCAGATATGTTTGATTTGCCATCTGACCCCACCACCGACAATATGGATTTCATATACAGTCCCCAGTCTGAGCTGAATCATGTGCAAGAATTGGAGAAAATGTTTAACATTCCACCTATAGATATCATTCTTAATGATGAACTTCTCCGTTCTTCAACCCAAATATGGCTCCAGCATTTTCAGCGGGAATATAGAGTCAACAGAGTTAAAAGGTCCCTTTGCATCACCCCTGTAGTTCCTTTCCAGCTAATGAAATTACCCAATCTATACCAGGACCTACTACAAAGGTGTATAAAAAAACGTTGCGTCAACTGCACCAAAGTTATCGAAGAACCTGTATTATGTCTTCTGTGTGGAAGTTTGTGTTCTCCCATATGGAGCCCTTGTTGCAGAGAAAGTGGTTGTCCTAATCATGCAATTACCTGTGGTGCTGGTACTGGTGTATTTCTTCTGATACGGCGGACCACAATTTTGCTACAAAGGTTTGCAAGACAATCACCTTGGCCATCTCCTTACCTGGATACGTTTGGAGAAGAGGATATCGATATGATCAGAGGAAAACGACTATACCTGAATGAAGAACGTTATGCTGCTTTAACTTACTTGGTTGGTTCTCATGGACTTGATCGCAGTTCAAAAGTTCTCGGCCAAACCACAATTGGAGCCGTCTTACATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000151 GO:0000280 GO:0001101 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006259 GO:0006310 GO:0006325 GO:0006342 GO:0006355 GO:0006464 GO:0006508 GO:0006511 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0007049 GO:0007127 GO:0007131 GO:0007140 GO:0007141 GO:0007276 GO:0007283 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009605 GO:0009607 GO:0009620 GO:0009719 GO:0009725 GO:0009737 GO:0009889 GO:0009890 GO:0009892 GO:0009894 GO:0009966 GO:0009968 GO:0009987 GO:0010029 GO:0010033 GO:0010243 GO:0010468 GO:0010498 GO:0010528 GO:0010529 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010646 GO:0010648 GO:0016043 GO:0016458 GO:0016567 GO:0016569 GO:0016570 GO:0016574 GO:0016597 GO:0016740 GO:0019216 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0019941 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0023051 GO:0023057 GO:0030163 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031406 GO:0032006 GO:0032007 GO:0032446 GO:0032501 GO:0032504 GO:0032991 GO:0033522 GO:0033993 GO:0034641 GO:0035825 GO:0036094 GO:0036211 GO:0040029 GO:0042221 GO:0043161 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043177 GO:0043200 GO:0043201 GO:0043207 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0044703 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048232 GO:0048285 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0048609 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0050994 GO:0051171 GO:0051172 GO:0051239 GO:0051252 GO:0051253 GO:0051276 GO:0051321 GO:0051603 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0061630 GO:0061659 GO:0061982 GO:0065007 GO:0070647 GO:0070728 GO:0070887 GO:0071229 GO:0071230 GO:0071233 GO:0071310 GO:0071417 GO:0071495 GO:0071596 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097305 GO:0098542 GO:0140013 GO:0140096 GO:1900140 GO:1901360 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902494 GO:1902531 GO:1902532 GO:1902679 GO:1903046 GO:1903506 GO:1903507 GO:1990234 GO:2000026 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-UBR

Protein Analysis

2006

Amino Acids

223.96

Weight (kDa)

6.2

Isoelectric Point (pI)

55.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-UBR PF02207 38 - 105 1.6e-20 Putative zinc finger in N-recognin (UBR box)
WHD_UBR1 PF22960 787 - 873 4e-19 E3 ubiquitin-protein ligase UBR1-like, winged-helix domain
PRT6_C PF18995 1461 - 1902 8e-92 Proteolysis_6 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 2726, 4768
Acc36I ACCTGC 2 cut(s) 4953, 5169
AccB1I GGYRCC 1 cut(s) 1060
AccBSI CCGCTC 1 cut(s) 99
AccI GTMKAC 3 cut(s) 2026, 3494, 4456
AccII CGCG 2 cut(s) 1677, 2857
AccIII TCCGGA 1 cut(s) 2497
AciI CCGC 8 cut(s) 97, 1758, 1890, 2433, 3244, 4318, 5527, 5803
AclI AACGTT 2 cut(s) 5644, 5926
AcoI YGGCCR 6 cut(s) 138, 1790, 2045, 3241, 5846, 5989
AcuI CTGAAG 6 cut(s) 1635, 2194, 3323, 3756, 4464, 4494
AcvI CACGTG 3 cut(s) 3020, 4007, 5040
AcyI GRCGYC 1 cut(s) 4120
AfiI CCNNNNNNNGG 9 cut(s) 457, 1871, 1889, 2924, 3263, 3364, 4130, 4758, 5508
AflII CTTAAG 1 cut(s) 4652
AflIII ACRYGT 4 cut(s) 1207, 2266, 4004, 4006
AgeI ACCGGT 1 cut(s) 2103
AjuI GAANNNNNNNTTGG 4 cut(s) 1197, 1229, 5133, 5165
AleI CACNNNNGTG 1 cut(s) 5002
Alw21I GWGCWC 5 cut(s) 2703, 3025, 3745, 4273, 4799
Alw44I GTGCAC 1 cut(s) 4269
AlwNI CAGNNNCTG 1 cut(s) 2563
Ama87I CYCGRG 1 cut(s) 3359
Aor13HI TCCGGA 1 cut(s) 2497
ApaLI GTGCAC 1 cut(s) 4269
ArsI GACNNNNNNTTYG 6 cut(s) 1211, 1243, 3476, 3508, 5797, 5829
AseI ATTAAT 1 cut(s) 911
AsiGI ACCGGT 1 cut(s) 2103
Asp700I GAANNNNTTC 7 cut(s) 576, 759, 2268, 2764, 3308, 3790, 4298
AspA2I CCTAGG 1 cut(s) 5324
AspLEI GCGC 1 cut(s) 3423
AsuC2I CCSGG 3 cut(s) 137, 1277, 3865
AsuHPI GGTGA 6 cut(s) 1096, 1872, 4139, 4502, 5560, 5832
AsuNHI GCTAGC 1 cut(s) 1102
AvaI CYCGRG 1 cut(s) 3359
AvaII GGWCC 8 cut(s) 868, 1931, 2530, 4064, 4526, 5556, 5617, 5805
AvrII CCTAGG 1 cut(s) 5324
BaeGI GKGCMC 2 cut(s) 2296, 4273
BaeI ACNNNNGTAYC 6 cut(s) 693, 726, 2994, 3027, 5769, 5802
BalI TGGCCA 2 cut(s) 1792, 5848
BanI GGYRCC 1 cut(s) 1060
BanII GRGCYC 6 cut(s) 2475, 2703, 3745, 4799, 5011, 5726
BarI GAAGNNNNNNTAC 2 cut(s) 5673, 5705
BbrPI CACGTG 3 cut(s) 3020, 4007, 5040
BbsI GAAGAC 6 cut(s) 919, 2540, 3230, 5012, 5264, 5681
Bbv12I GWGCWC 5 cut(s) 2703, 3025, 3745, 4273, 4799
BceAI ACGGC 3 cut(s) 233, 5816, 5993
BcgI CGANNNNNNTGC 2 cut(s) 2812, 2846
BciVI GTATCC 1 cut(s) 5857
BclI TGATCA 2 cut(s) 346, 5892
BcnI CCSGG 3 cut(s) 137, 1277, 3865
BfrI CTTAAG 1 cut(s) 4652
BfuAI ACCTGC 2 cut(s) 4953, 5169
BfuI GTATCC 1 cut(s) 5857
BglII AGATCT 2 cut(s) 3530, 4540
BlnI CCTAGG 1 cut(s) 5324
BmcAI AGTACT 2 cut(s) 1870, 4658
Bme18I GGWCC 8 cut(s) 868, 1931, 2530, 4064, 4526, 5556, 5617, 5805
BmeT110I CYCGRG 1 cut(s) 3359
BmiI GGNNCC 6 cut(s) 84, 1062, 5513, 5558, 5723, 6007
BmrI ACTGGG 1 cut(s) 5401
BmtI GCTAGC 1 cut(s) 1106
BmuI ACTGGG 1 cut(s) 5401
BpiI GAAGAC 6 cut(s) 919, 2540, 3230, 5012, 5264, 5681
BplI GAGNNNNNCTC 3 cut(s) 28, 143, 175
BpmI CTGGAG 9 cut(s) 785, 1842, 2393, 2658, 2687, 3635, 4374, 4783, 5498
Bpu10I CCTNAGC 1 cut(s) 1705
BpuEI CTTGAG 6 cut(s) 1302, 2408, 2461, 3159, 3520, 5302
BpuMI CCSGG 3 cut(s) 137, 1277, 3865
Bsa29I ATCGAT 1 cut(s) 5886
BsaAI YACGTR 6 cut(s) 2024, 3020, 4007, 4394, 4406, 5040
BsaBI GATNNNNATC 2 cut(s) 2232, 3522
BsaHI GRCGYC 1 cut(s) 4120
BsaJI CCNNGG 8 cut(s) 1488, 1793, 2295, 2755, 2894, 3360, 5324, 5843
BsaWI WCCGGW 2 cut(s) 2103, 2497
Bsc4I CCNNNNNNNGG 9 cut(s) 457, 1871, 1889, 2924, 3263, 3364, 4130, 4758, 5508
Bse118I RCCGGY 1 cut(s) 2103
Bse8I GATNNNNATC 2 cut(s) 2232, 3522
BseAI TCCGGA 1 cut(s) 2497
BseCI ATCGAT 1 cut(s) 5886
BseDI CCNNGG 8 cut(s) 1488, 1793, 2295, 2755, 2894, 3360, 5324, 5843
BseJI GATNNNNATC 2 cut(s) 2232, 3522
BseLI CCNNNNNNNGG 9 cut(s) 457, 1871, 1889, 2924, 3263, 3364, 4130, 4758, 5508
BseRI GAGGAG 2 cut(s) 3786, 5299
BseSI GKGCMC 2 cut(s) 2296, 4273
BsgI GTGCAG 3 cut(s) 2235, 3030, 5641
Bsh1236I CGCG 2 cut(s) 1677, 2857
BshNI GGYRCC 1 cut(s) 1060
BshTI ACCGGT 1 cut(s) 2103
BshVI ATCGAT 1 cut(s) 5886
BsiHKAI GWGCWC 5 cut(s) 2703, 3025, 3745, 4273, 4799
BsiHKCI CYCGRG 1 cut(s) 3359
BsiSI CCGG 7 cut(s) 137, 1276, 2048, 2104, 2498, 2745, 3865
BslFI GGGAC 7 cut(s) 390, 2397, 3193, 4658, 4774, 5388, 5542
BslI CCNNNNNNNGG 9 cut(s) 457, 1871, 1889, 2924, 3263, 3364, 4130, 4758, 5508
BsmFI GGGAC 7 cut(s) 390, 2397, 3193, 4658, 4774, 5388, 5542
BsmI GAATGC 4 cut(s) 840, 1010, 2014, 3769
BsoBI CYCGRG 1 cut(s) 3359
Bsp1286I GDGCHC 9 cut(s) 2296, 2475, 2703, 3025, 3745, 4273, 4799, 5011, 5726
Bsp13I TCCGGA 1 cut(s) 2497
Bsp1407I TGTACA 1 cut(s) 4195
BspACI CCGC 8 cut(s) 97, 1758, 1890, 2433, 3244, 4318, 5527, 5803
BspDI ATCGAT 1 cut(s) 5886
BspEI TCCGGA 1 cut(s) 2497
BspFNI CGCG 2 cut(s) 1677, 2857
BspHI TCATGA 3 cut(s) 1552, 2185, 5335
BspLI GGNNCC 6 cut(s) 84, 1062, 5513, 5558, 5723, 6007
BspMAI CTGCAG 5 cut(s) 285, 667, 2842, 3054, 4425
BspMI ACCTGC 2 cut(s) 4953, 5169
BspOI GCTAGC 1 cut(s) 1106
BspT107I GGYRCC 1 cut(s) 1060
BspTI CTTAAG 1 cut(s) 4652
BsrBI CCGCTC 1 cut(s) 99
BsrFI RCCGGY 1 cut(s) 2103
BsrGI TGTACA 1 cut(s) 4195
BssAI RCCGGY 1 cut(s) 2103
BssECI CCNNGG 8 cut(s) 1488, 1793, 2295, 2755, 2894, 3360, 5324, 5843
BssNAI GTATAC 1 cut(s) 2027
BssNI GRCGYC 1 cut(s) 4120
BssT1I CCWWGG 4 cut(s) 1488, 1793, 5324, 5843
Bst1107I GTATAC 1 cut(s) 2027
Bst6I CTCTTC 9 cut(s) 19, 826, 1756, 2605, 3210, 3887, 4734, 5334, 5871
BstACI GRCGYC 1 cut(s) 4120
BstAFI CTTAAG 1 cut(s) 4652
BstAUI TGTACA 1 cut(s) 4195
BstBAI YACGTR 6 cut(s) 2024, 3020, 4007, 4394, 4406, 5040
BstC8I GCNNGC 6 cut(s) 44, 69, 440, 1104, 2931, 3447
BstFNI CGCG 2 cut(s) 1677, 2857
BstHHI GCGC 1 cut(s) 3423
BstMWI GCNNNNNNNGC 9 cut(s) 137, 1108, 2426, 3458, 3603, 5114, 5524, 5655, 5730
BstNSI RCATGY 7 cut(s) 411, 1381, 1704, 2201, 2270, 4193, 5121
BstSLI GKGCMC 2 cut(s) 2296, 4273
BstSNI TACGTA 2 cut(s) 2024, 4406
BstUI CGCG 2 cut(s) 1677, 2857
BstV2I GAAGAC 6 cut(s) 919, 2540, 3230, 5012, 5264, 5681
BstX2I RGATCY 8 cut(s) 692, 919, 1861, 2190, 2797, 3530, 4282, 4540
BstXI CCANNNNNNTGG 1 cut(s) 2240
BstYI RGATCY 8 cut(s) 692, 919, 1861, 2190, 2797, 3530, 4282, 4540
BstZ17I GTATAC 1 cut(s) 2027
Bsu15I ATCGAT 1 cut(s) 5886
BsuI GTATCC 1 cut(s) 5857
BsuTUI ATCGAT 1 cut(s) 5886
BtgZI GCGATG 1 cut(s) 3918
BtsI GCAGTG 3 cut(s) 290, 3047, 4418
BveI ACCTGC 2 cut(s) 4953, 5169
Cac8I GCNNGC 6 cut(s) 44, 69, 440, 1104, 2931, 3447
CaiI CAGNNNCTG 1 cut(s) 2563
CciI TCATGA 3 cut(s) 1552, 2185, 5335
CfoI GCGC 1 cut(s) 3423
Cfr10I RCCGGY 1 cut(s) 2103
ClaI ATCGAT 1 cut(s) 5886
CseI GACGC 2 cut(s) 4109, 5638
CspAI ACCGGT 1 cut(s) 2103
CspCI CAANNNNNGTGG 2 cut(s) 2795, 2830
DraI TTTAAA 2 cut(s) 1335, 4224
DrdI GACNNNNNNGTC 2 cut(s) 2726, 4768
DseDI GACNNNNNNGTC 2 cut(s) 2726, 4768
EaeI YGGCCR 6 cut(s) 138, 1790, 2045, 3241, 5846, 5989
Eam1104I CTCTTC 9 cut(s) 19, 826, 1756, 2605, 3210, 3887, 4734, 5334, 5871
EarI CTCTTC 9 cut(s) 19, 826, 1756, 2605, 3210, 3887, 4734, 5334, 5871
EciI GGCGGA 1 cut(s) 5818
Ecl136II GAGCTC 3 cut(s) 2701, 3743, 4797
Eco105I TACGTA 2 cut(s) 2024, 4406
Eco130I CCWWGG 4 cut(s) 1488, 1793, 5324, 5843
Eco24I GRGCYC 6 cut(s) 2475, 2703, 3745, 4799, 5011, 5726
Eco32I GATATC 4 cut(s) 1132, 3729, 5467, 5884
Eco47I GGWCC 8 cut(s) 868, 1931, 2530, 4064, 4526, 5556, 5617, 5805
Eco53kI GAGCTC 3 cut(s) 2701, 3743, 4797
Eco57I CTGAAG 6 cut(s) 1635, 2194, 3323, 3756, 4464, 4494
Eco72I CACGTG 3 cut(s) 3020, 4007, 5040
Eco88I CYCGRG 1 cut(s) 3359
EcoICRI GAGCTC 3 cut(s) 2701, 3743, 4797
EcoO109I RGGNCCY 3 cut(s) 3686, 5556, 5617
EcoRI GAATTC 3 cut(s) 119, 4298, 4699
EcoRV GATATC 4 cut(s) 1132, 3729, 5467, 5884
EcoT14I CCWWGG 4 cut(s) 1488, 1793, 5324, 5843
EcoT22I ATGCAT 3 cut(s) 1012, 1383, 2014
EcoT38I GRGCYC 6 cut(s) 2475, 2703, 3745, 4799, 5011, 5726
ErhI CCWWGG 4 cut(s) 1488, 1793, 5324, 5843
FalI AAGNNNNNCTT 8 cut(s) 1966, 1998, 3852, 3884, 4096, 4128, 4873, 4905
FaqI GGGAC 7 cut(s) 390, 2397, 3193, 4658, 4774, 5388, 5542
FauI CCCGC 2 cut(s) 2440, 5520
FauNDI CATATG 1 cut(s) 5717
FbaI TGATCA 2 cut(s) 346, 5892
FblI GTMKAC 3 cut(s) 2026, 3494, 4456
FriOI GRGCYC 6 cut(s) 2475, 2703, 3745, 4799, 5011, 5726
GlaI GCGC 1 cut(s) 3422
GsuI CTGGAG 9 cut(s) 785, 1842, 2393, 2658, 2687, 3635, 4374, 4783, 5498
HapII CCGG 7 cut(s) 137, 1276, 2048, 2104, 2498, 2745, 3865
HgaI GACGC 2 cut(s) 4109, 5638
HhaI GCGC 1 cut(s) 3423
Hin1I GRCGYC 1 cut(s) 4120
Hin6I GCGC 1 cut(s) 3421
HinP1I GCGC 1 cut(s) 3421
HincII GTYRAC 4 cut(s) 1299, 2880, 5542, 5653
HindII GTYRAC 4 cut(s) 1299, 2880, 5542, 5653
HindIII AAGCTT 2 cut(s) 2453, 5330
HpaII CCGG 7 cut(s) 137, 1276, 2048, 2104, 2498, 2745, 3865
HphI GGTGA 6 cut(s) 1096, 1872, 4139, 4502, 5560, 5832
HpyAV CCTTC 9 cut(s) 804, 1081, 1662, 1712, 2782, 4259, 4437, 4527, 4907
HpyF10VI GCNNNNNNNGC 9 cut(s) 137, 1108, 2426, 3458, 3603, 5114, 5524, 5655, 5730
Hsp92I GRCGYC 1 cut(s) 4120
HspAI GCGC 1 cut(s) 3421
Kpn2I TCCGGA 1 cut(s) 2497
Ksp22I TGATCA 2 cut(s) 346, 5892
MaeIII GTNAC 7 cut(s) 1957, 2106, 2678, 3054, 4401, 4507, 5058
MbiI CCGCTC 1 cut(s) 99
MfeI CAATTG 4 cut(s) 2657, 3318, 3809, 6000
MflI RGATCY 8 cut(s) 692, 919, 1861, 2190, 2797, 3530, 4282, 4540
MhlI GDGCHC 9 cut(s) 2296, 2475, 2703, 3025, 3745, 4273, 4799, 5011, 5726
MlsI TGGCCA 2 cut(s) 1792, 5848
MluNI TGGCCA 2 cut(s) 1792, 5848
MlyI GAGTC 9 cut(s) 247, 1961, 2181, 3220, 3842, 4721, 4868, 5351, 5547
MmeI TCCRAC 7 cut(s) 623, 720, 1161, 1519, 2673, 2765, 5202
Mox20I TGGCCA 2 cut(s) 1792, 5848
Mph1103I ATGCAT 3 cut(s) 1012, 1383, 2014
MroI TCCGGA 1 cut(s) 2497
MroXI GAANNNNTTC 7 cut(s) 576, 759, 2268, 2764, 3308, 3790, 4298
MscI TGGCCA 2 cut(s) 1792, 5848
MslI CAYNNNNRTG 7 cut(s) 275, 397, 1885, 2061, 2238, 4670, 5002
Msp20I TGGCCA 2 cut(s) 1792, 5848
MspA1I CMGCKG 2 cut(s) 1964, 5527
MspCI CTTAAG 1 cut(s) 4652
MspI CCGG 7 cut(s) 137, 1276, 2048, 2104, 2498, 2745, 3865
MunI CAATTG 4 cut(s) 2657, 3318, 3809, 6000
Mva1269I GAATGC 4 cut(s) 840, 1010, 2014, 3769
MvnI CGCG 2 cut(s) 1677, 2857
MwoI GCNNNNNNNGC 9 cut(s) 137, 1108, 2426, 3458, 3603, 5114, 5524, 5655, 5730
NciI CCSGG 3 cut(s) 137, 1277, 3865
NdeI CATATG 1 cut(s) 5717
NheI GCTAGC 1 cut(s) 1102
NlaIV GGNNCC 6 cut(s) 84, 1062, 5513, 5558, 5723, 6007
NmeAIII GCCGAG 1 cut(s) 5967
NmuCI GTSAC 2 cut(s) 2678, 5058
NsiI ATGCAT 3 cut(s) 1012, 1383, 2014
NspI RCATGY 7 cut(s) 411, 1381, 1704, 2201, 2270, 4193, 5121
OliI CACNNNNGTG 1 cut(s) 5002
PagI TCATGA 3 cut(s) 1552, 2185, 5335
PciI ACATGT 1 cut(s) 2266
PcsI WCGNNNNNNNCGW 1 cut(s) 2365
PctI GAATGC 4 cut(s) 840, 1010, 2014, 3769
PdmI GAANNNNTTC 7 cut(s) 576, 759, 2268, 2764, 3308, 3790, 4298
PfoI TCCNGGA 5 cut(s) 297, 2532, 2703, 3284, 4351
PinAI ACCGGT 1 cut(s) 2103
PleI GAGTC 9 cut(s) 246, 1961, 2180, 3220, 3841, 4721, 4867, 5350, 5546
PmaCI CACGTG 3 cut(s) 3020, 4007, 5040
PmlI CACGTG 3 cut(s) 3020, 4007, 5040
PpsI GAGTC 9 cut(s) 246, 1961, 2180, 3220, 3841, 4721, 4867, 5350, 5546
Ppu21I YACGTR 6 cut(s) 2024, 3020, 4007, 4394, 4406, 5040
PpuMI RGGWCCY 2 cut(s) 5556, 5617
PscI ACATGT 1 cut(s) 2266
PshBI ATTAAT 1 cut(s) 911
Psp124BI GAGCTC 3 cut(s) 2703, 3745, 4799
Psp1406I AACGTT 2 cut(s) 5644, 5926
Psp5II RGGWCCY 2 cut(s) 5556, 5617
PspCI CACGTG 3 cut(s) 3020, 4007, 5040
PspN4I GGNNCC 6 cut(s) 84, 1062, 5513, 5558, 5723, 6007
PspPPI RGGWCCY 2 cut(s) 5556, 5617
PsrI GAACNNNNNNTAC 4 cut(s) 2256, 2288, 4795, 4827
PstI CTGCAG 5 cut(s) 285, 667, 2842, 3054, 4425
PstNI CAGNNNCTG 1 cut(s) 2563
PsuI RGATCY 8 cut(s) 692, 919, 1861, 2190, 2797, 3530, 4282, 4540
PvuII CAGCTG 1 cut(s) 1964
RseI CAYNNNNRTG 7 cut(s) 275, 397, 1885, 2061, 2238, 4670, 5002
SacI GAGCTC 3 cut(s) 2703, 3745, 4799
ScaI AGTACT 2 cut(s) 1870, 4658
SchI GAGTC 9 cut(s) 247, 1961, 2181, 3220, 3842, 4721, 4868, 5351, 5547
SduI GDGCHC 9 cut(s) 2296, 2475, 2703, 3025, 3745, 4273, 4799, 5011, 5726
SinI GGWCC 8 cut(s) 868, 1931, 2530, 4064, 4526, 5556, 5617, 5805
SmiMI CAYNNNNRTG 7 cut(s) 275, 397, 1885, 2061, 2238, 4670, 5002
SmlI CTYRAG 7 cut(s) 1317, 2387, 2476, 3174, 3499, 4652, 5281
SmoI CTYRAG 7 cut(s) 1317, 2387, 2476, 3174, 3499, 4652, 5281
SnaBI TACGTA 2 cut(s) 2024, 4406
SsiI CCGC 8 cut(s) 97, 1758, 1890, 2433, 3244, 4318, 5527, 5803
SspI AATATT 1 cut(s) 3277
SstI GAGCTC 3 cut(s) 2703, 3745, 4799
StyI CCWWGG 4 cut(s) 1488, 1793, 5324, 5843
TaqII GACCGA 1 cut(s) 2349
TatI WGTACW 4 cut(s) 1568, 1868, 4195, 4656
TauI GCSGC 4 cut(s) 1761, 1893, 3246, 4320
TseFI GTSAC 2 cut(s) 2678, 5058
Tsp45I GTSAC 2 cut(s) 2678, 5058
TspGWI ACGGA 4 cut(s) 2206, 3575, 5249, 5480
Vha464I CTTAAG 1 cut(s) 4652
VneI GTGCAC 1 cut(s) 4269
VpaK11BI GGWCC 8 cut(s) 868, 1931, 2530, 4064, 4526, 5556, 5617, 5805
VspI ATTAAT 1 cut(s) 911
XbaI TCTAGA 1 cut(s) 806
XceI RCATGY 7 cut(s) 411, 1381, 1704, 2201, 2270, 4193, 5121
XcmI CCANNNNNNNNNTGG 4 cut(s) 2303, 2540, 5383, 5999
XmaJI CCTAGG 1 cut(s) 5324
XmiI GTMKAC 3 cut(s) 2026, 3494, 4456
XmnI GAANNNNTTC 7 cut(s) 576, 759, 2268, 2764, 3308, 3790, 4298
ZrmI AGTACT 2 cut(s) 1870, 4658
Zsp2I ATGCAT 3 cut(s) 1012, 1383, 2014
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.