RLG00000024565

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0007182
Physical Location & Seq
Reverse (-)
37049338 .. 37059240
9903 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024565

Sequence Viewer

Length: 6624 bp
ATGGATATCGATTCCCCTTCTGAATCTCCCCCTCGTGATCGAATCGTTCAGAGGCTTGCTCGACTGGGAGTGCCTCAGAATCTTCTTCATCTGCATCAACTTGGTTTAGTTGCTTTTGTTAAAAATAACAAGCGGTTGTTACCGCAACTGGTTTCTGCTATCTTACCTCCTGATGAGGAAGTAGCGGAAGTTCTCCGCGAGGCCAAACCAGGGTCGAAGAAACAATCAGCCGGTGTGACCATGAAGCTTCAATTCCGCGAAAGCATCTCGTGGCTAAAGTGGTTGATGTTTGAGAGTGACCCTGGCTTGGTTCTGAAGGGCCTGTCCAAAATGAGCGTCGGGCAGCGGGGCATTTGTGGAGCTGTTTGGGGACATAATGATATAGCCTTCAGGTGCAAGACGTGCGAACATGATCCAACCTGTGCAATTTGCGTTCCCTGTTTTAAGAATGGGAACCACAAGGATCATGATTACTCTATTATCTATACCGGCGGTGGCTGTTGTGATTGCGGTGATGTTACAGCATGGAAACGCCAAGGCTTTTGCTCAAAGCATAAAGGTGCAGAACAGATACAGCCCCTTCCAGAGGAGCTTGCAATCACCGTGGGGCCTGTTCTTGACTACCTCTTTGCCTATTGGAAGAACAAGCTACTTCTTTCTGAAACTGCCTGCCGGGAAAACCCTAGAGTAACTGACCTTGCTGCAGAGCGTAAGAAGGTTGCAAATGAGCTAACCTATTTTGTGGTTGAGATGCTTCTGGACTTTTGCAAGTGCAGTGAGAGTTTGCTTAGTTTTGTATCAAAGATGATTTTATCATCAGTTGACTTTTTGGGGATTTTGGTGAGGGCAGAGAGGTTTTTGAGTGATGCTGTTGTGAAAAAGCTCCATGAGTTGCTCCTGAAATTGATGGGGGAGCCTGTTTTCAAATATGAGTTTGCCAAAGTATTTCTTAATTATTACCCAACTGTTGTTAGTGAAGCCATAAAGGAGTTCACTGACCTTACTTTGAAAAAGTATCCGCTGCTCTCTATATTCTCTGTGCAAATCCTCACGGTGCCAACTTTAACACCCCGTCTTGTCAAAGAAATGAACCTACTGTCGATGCTGTTAGGATGTTTGGAAGATATTTTCAATTCCTGTGCTGGTGAAGACGCCCGTTTACAGGTTACCAAGTGGTCAAATTTGTATGACGTCACTATTCGTGTGATTGAAGATTTTCGGTTTGTTATGAGTCATGCTGTAGTGCCAAAATATGTAACTCATGGGCAGCAAGATATTGCGAGAACTTGGATGAGACTTTCAGCTTTTGTGCAAGGGATGAACCCACAAAAGAGGGAAACAGGCCTTCATATTGAAGACGAGAATGACCATATGCATTTGCCATTTGTTCTGGGTCATTCAATCGCCAACCTTCATTCTTTGTTGGTGGATGGAGCATTTTCTGTTGCTAGTGACAAGACAGAAGAAGACTTATTTCTCGGCACAAATAAGCAAGATATGGATGACACAGATAGCCTAAGACATGCAAAAGTAGGACGGCTGTCCCAGGAAAGTTCTGCATGTAGTGCAGTGGGGAGTAGTTCATTTGCATGTGCATTGACGTTTTCTGAAGATAAATCAAATGCTCTTTCGACGATACCATCATCTGTGGTATGGTTAACATTTGAGTGTTTGAGGGCTATTGAGAATTGGCTGGGAGTTGATAACACTTCGGGTACAATTCTTCATGTCTCATCTTCGAGTACTAGCAATTTCTCTGGGAGCAATTTTTCAGCATTGAAGAGAACATTATCCAAAATTAGGAAAGGCAAAAACATTTTTGGTCGATTTGCCAGTTCAAGTGAAGACCATGGGAAGCATAGTTCATCACATTTACATAGTAGTTCTGATATGAGTGTTGACTTCCAGAATGGCAAAATTTCAGAAAATAAATTAATGGTTATGGACGAAATCGATCAAGCTAATGCCTGTAATTCGTCAGGATCTGGTGATAGTGCAATGGAAATAGATGGTCCCATGGACTTAGATGCTCTACGCGTGCTGAGTTCATCCGACTGGCCAGACATAACATATGATGTTAGCTCGCAGGATATATCTGTTCACATTCCCTTACATCGCTTACTTGCATTGCTTCTACAGAGATCATTAAGAAGGTGTTTTGGCGAAGTGCCAGATTCTGTTAGAGCTAGTTCTGCAAATTTATCATCATCTATTTCCAGAGACTTTTTTGGAAATATTCTCGGAGGCTGCCACCCATGTGGATTTTCTGCCTTTGTTATGGAGCATCCCCTTCGTATAAGAGTATTTTGTGCTGAGGTTCACGCTGGAATGTGGCGGAAAAATGGGGATGCTGCCCCATTAACTTGTGAGTGGTATCGTTCAGTTCGCTGGTCGGAACAGTTTGTGGAGCTTGATCTATTTCTGCTTCAGTGCTGTGCTGCCTTGGCTCCAGCTGATCTTTATATCAAGAGAATCATAGAACGCTTTGGCCTGTCAAGCTACCTTTCTCTGAACCTTGAGCGTTCTAGTGAATATGAACCAGTTCTAGTGCAGGAAATGCTCACTCTTATTATACAAATAATCAAAGAAAGGCGATTCTGTGGGTTAACTAAAGCTGAAAGTGTGAAAAGAGAATTGATTCATAAGTTAAGCATCGCAGATGCTACTCACAGTCAATTGGTTAAGTCTCTTCCTCGTGATCTTACCAAGTATGACCAGCTTAATGAAATTTTGGATACAGTTGCTGTGTATTCCAATCCCTCTGGCTTTAACCAGGGGACTTATTCACTACAATGGACATTTTGGGAAGAACTGGATTTGTACTACCATCGTTGGAACTCAAGGGATCTACAAGCTGCAGAAGAAAGATACTTACGTTTCCGTAGCATTTCTGCATTGACCAATCAGCTACCCAGGTGGACTAAGATTTATCCTCCTTTCAGGGGGTTAGCTAGAATTGGGACTTGTAAAACCGTCCTCCAAATTGTTCGCGCGGTGCTGTTTTATGCTGTATTTTCTGATAAATCGGTTGAGTCACGAGCGCCTGATGGGGTCCTCTTAACTGCATTGCACTTGCTCTCATTAGCATTAGACATTTGTTCTCAACATAAAGAATCCGGTGACCATTCCTGTTATGATGGAGATTTCATTCCAGTGCTTGCTTTTGCCTGTGAAGAAATTAATGAAGGACTCTATTTTGAGGCTGGTCAACAAAGCTTGTTGTCACTTCTTGTTATTTTGATGAGGATGTATTCGAAAGAGGGTTTAGATAATTCAGAAGATGGAAGTTGCAACCTTTCTTCTTTAATCGGAAACTTACTGAAGAAGTTTGCATTTATTGATTGTGAATGTATGACCAAACTGCAGGTACTTGCACCTGAGTTGGTTAGTCATGTAACTTTTCCAAATAGTGATACAGTTACCTCAGGTTCAGCTTCTGATTCTGAGAAGCGGAAAGCAAAAGCCAGAGCGAGACAGGCTGCCATACTGGAAAAAATGAGAGCCGAGCAATCAAAGTTTTTGAGTAGTATTGATTCCTCTGTGGATGATGGTTCAGAAGCTGAACAAAAGGATGTTGAATCTGATGTTGAAGATAATTCAGAAGAGCCTGCACAAGTTGTTTGCTCACTCTGCCATGATCCGAATTCCAAGAATCCTATTTCCTTCTTGGTTCTTCTTCAAAAGTCTAGGCTTTTGAGTTTCATTGATAGAGGTCCACTATCATGGGAACAACCTCGCTCCGTAGACAAGGAGCATGTTGTTAAAACCAAAGATGAGGTGATTGATCAATCTGGTATAAGCAGATTGTTTAGTGGTTCAGGATTTGTTTCATCGTCTCATGTAAGACAGTTGGTGTCGGAACACTACAACAGAAAAAAGGTGAGTGGTGAATCTGGAACAAGTACACTGTCAAGTGGTTCAGGAGTGGTGTCGCCTCATCATCTAAGGCAGTTGGTTGAGAAGGCAGTCACTGAGTTTGCCCGACATGGGAGACCTAGGGATGTTGGTGCCTTTCTAGAAGTTCTGAAGGGCCGGTTTCATGACTTAAGGAATTTCCAAGTGCCATGTGAATCAAATGATGAAAGGGAAAGTACTTCGTATGCCTTTGAGACAATGGAAGAAGATATGTACTTCTCTATCCGAAGAGAACTGCACAATAAACTACATTCTAAACTTACAGAAGATAAGAAGTGTACAACTGCTGAAGGGGATCGAGAGAATACAGAGCATACTGAATTTATGTTACTTGGAAACTATATAGCTGCTCTTTCTAGAGAGAGAAGAGAAGATCCTTCATCTTCTGAAAGTGATCCTAATGATAAGGGGCCAGTAGAATGTTCTCGCCTGTCAGCATATGATGGATTTGGCCCTGCAGATTGTGATGGAATTTATATTTCTTCCTGTGGGCATGCTGTGCATCAGGAATGTCTTGATCGTTATTTGTCTTCCCTGAAGGAAAGGTATCTCAGGAGAATTGTTTTTGAAGGAGGACATATTGTAGATCCAGACAAGGGTGAATTTCTTTGCCCTGTATGCCGTCGACTTGCAAATTCAGTCTTGCCTACATTACCAAGTGAGTTCCAGAAGGTTCGCAAAGAGCCTATGGATGCAGGTGTTAGTTCATCACATGTTACTAGTCCATCATGCAAACCAGGCGAAGGAATTAGTTCTTTTCAGCAAGGCTTGGCTCTCTTGCAATCTGCAGCAAATGCAGCTGGGAAGGTTGGCACGCTCAAAGGTTTCCCCCTGCATAGATGTGAACAAATGAGCTCAAATCTTGAACCAATCTCACGTCTGCTATCTAAGATGTATTTCCCAACTGATCTGGATAAGATATCAGGATCTGACAGGGTCAGCCACCCAATGCTTATGTGGGACTTAATTAAGTACTCTCTACTGTCAATGGAGATTGCTTCTCGTTCTGGGGGGAAATATGCAGCACCAAGCTACAGCCTTAATGCCTTGTATAAGGAACTTGAATCCTCGAGCAGATTCATATTGTCCTTGTTGCTGAAACTTGTCCAAAACACGTGCAAGAATTCTCTTCATGTGCTTCAGAGATTTATAGCAATTCAGAGCTTTGCAGAATCTATGTGCTTTGGAGTTTCCATAGATCATGGAAGCAAAACATCAGGACAAGTTGTTATGCTGCGTATCTTGGAACATGTTGATAATCCAGTAGCATATCCTGATTTCGAGTTCTGGAATCGAGCTTCTGATCCTGTGCTTGCCCGTGATCCTTTTTCTTCATTGATGTGGGTTCTATTTTGTCTTCCATACCGATTTTTATCATGTGAAGATTCCTTGCTATCCCTGGTGCATGTTTTTTATGTTGTTTCTGTGGTTCAGGGTATTGCTACATATTTTGGGAAAAATCAATGCGACATTAGTGGATTAGGTGTTGGTGATTGCCTGATTACTGACATCAGCAAGCTTATGAGTGAATTTGGATCCGCTCAGCAATATTTTGTATCAAACAATATAGACTTTTCTTATAATATTAAGAATATAGTTCGTACCTTGACTTTCCCTTATTTGCGGAGGTGTGCATTGTTGTTGAAACTACTCGAATCCTATGCCCAAGCGCCATTTTGTGAAAGGTATAATGCGTTGGATAGATCACGTGCCACTAGTGACGCAATGGATACCACTTACGTACCACTGGTCGAGCTCAATGAAATTCAAGAGATAGAAACCCTGCTCAAGATTCCTACTTTGGATGTTATTCTTAAGGATGAAGTGGTGCGTTCATTAGCTCATAAATGGTTCTGTCATTTTGGCAAAGAGTTTGAGGACAAAAGGTTTCAAGGCAGTATACACTGCAACCCTGCAGTTCCATTCCAGTTGATGCGTCTACCCCGAGTTTACCAGGATCTATTGCAGCGGTATATAAAGCAGCGGTGTCGTGACTGCAATAACATACTTGATGAACCTGCATTGTGCCTACTTTGTGGTAGGTTATGCTCTCCAAGCTGGAAGTCATGCTGCAGGGAAAGTGGATGCCAAACTCATGCAGTGGCTTGTGGTTCTGGTACCGGTGTCTTTCTCTTGATCAGGAGAACAACAATACTGCTGCAAAGATCTGCACGGCAGGCTCCTTGGCCATCACCCTACTTGGATGCTTTTGGTGAAGAGGACATTGAAATGCACAGAGGAAAGCCACTGTTCTTGAATGAGGAACGTTATGCAGCTTTAACTTATTTGTTCGAAGGTTCTTGGGCAAACTACTATTGGTTCTTTCTTCATGGTTTAGTTTGGATTATAAGATTGATGTCAACGGGATGGCAAAGAATTCTAGTTAACAGCAGGGAATATCCAATGCTCACAATGCTCACAATCAATCTTAATATTTGGGATTTTTACTGTATATTGAGATGCTTGGAGTTTCCTTTCCAGAGTTTTGACTATTGGTTCTACAGCAGACTAGTCTATTGCCAGGTTGTTCCTCCTGTTTTCAAGGTTGTACAGTTCAATGCGATGCTGGAAGCTGAAGATGCACACTCCAAACTCTCCTTTACTGGTGTGGAGTTAACCATCGAACTGTTGTACATATATATATTTTGCAACACTAGTCATGACCTCCGCTTTAAGGGTAAAGTGGTGGCATTTGCAATGCTTTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000151 GO:0000280 GO:0001101 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006259 GO:0006310 GO:0006325 GO:0006342 GO:0006355 GO:0006464 GO:0006508 GO:0006511 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0007049 GO:0007127 GO:0007131 GO:0007140 GO:0007141 GO:0007276 GO:0007283 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009605 GO:0009607 GO:0009620 GO:0009719 GO:0009725 GO:0009737 GO:0009889 GO:0009890 GO:0009892 GO:0009894 GO:0009966 GO:0009968 GO:0009987 GO:0010029 GO:0010033 GO:0010243 GO:0010468 GO:0010498 GO:0010528 GO:0010529 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010646 GO:0010648 GO:0016043 GO:0016458 GO:0016567 GO:0016569 GO:0016570 GO:0016574 GO:0016597 GO:0016740 GO:0019216 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0019941 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0023051 GO:0023057 GO:0030163 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031406 GO:0032006 GO:0032007 GO:0032446 GO:0032501 GO:0032504 GO:0032991 GO:0033522 GO:0033993 GO:0034641 GO:0035825 GO:0036094 GO:0036211 GO:0040029 GO:0042221 GO:0043161 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043177 GO:0043200 GO:0043201 GO:0043207 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0044703 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048232 GO:0048285 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0048609 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0050994 GO:0051171 GO:0051172 GO:0051239 GO:0051252 GO:0051253 GO:0051276 GO:0051321 GO:0051603 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0061630 GO:0061659 GO:0061982 GO:0065007 GO:0070647 GO:0070728 GO:0070887 GO:0071229 GO:0071230 GO:0071233 GO:0071310 GO:0071417 GO:0071495 GO:0071596 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097305 GO:0098542 GO:0140013 GO:0140096 GO:1900140 GO:1901360 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902494 GO:1902531 GO:1902532 GO:1902679 GO:1903046 GO:1903506 GO:1903507 GO:1990234 GO:2000026 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-UBR

Protein Analysis

2208

Amino Acids

248.63

Weight (kDa)

6.14

Isoelectric Point (pI)

49.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-UBR PF02207 119 - 185 2.7e-19 Putative zinc finger in N-recognin (UBR box)
WHD_UBR1 PF22960 875 - 962 4e-20 E3 ubiquitin-protein ligase UBR1-like, winged-helix domain
PRT6_C PF18995 1601 - 2068 9.4e-93 Proteolysis_6 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 5496, 6263
AarI CACCTGC 1 cut(s) 4601
AatII GACGTC 1 cut(s) 1194
Acc36I ACCTGC 3 cut(s) 3355, 4601, 5941
Acc65I GGTACC 1 cut(s) 6032
AccB1I GGYRCC 3 cut(s) 1054, 4007, 6032
AccBSI CCGCTC 1 cut(s) 5456
AccI GTMKAC 4 cut(s) 3744, 4540, 5815, 5854
AccII CGCG 5 cut(s) 198, 258, 2037, 2991, 2993
AclI AACGTT 1 cut(s) 6181
AcoI YGGCCR 2 cut(s) 2057, 6101
AcvI CACGTG 2 cut(s) 5031, 5624
AcyI GRCGYC 2 cut(s) 1152, 1191
AflII CTTAAG 2 cut(s) 4045, 5729
AflIII ACRYGT 4 cut(s) 2035, 4627, 5028, 5164
AgeI ACCGGT 1 cut(s) 6035
AhlI ACTAGT 4 cut(s) 4634, 5630, 6424, 6569
AjiI CACGTC 2 cut(s) 402, 4793
AjnI CCWGG 9 cut(s) 208, 301, 1545, 2772, 2912, 4651, 5313, 5868, 6435
AleI CACNNNNGTG 1 cut(s) 2256
AloI GAACNNNNNNTCC 2 cut(s) 1846, 1878
Alw21I GWGCWC 2 cut(s) 4772, 5673
Alw26I GTCTC 8 cut(s) 1288, 1735, 2214, 2691, 3466, 3840, 3985, 4103
AlwNI CAGNNNCTG 7 cut(s) 1985, 2177, 2744, 3437, 3560, 3971, 4844
Ama87I CYCGRG 2 cut(s) 4984, 5859
ArsI GACNNNNNNTTYG 4 cut(s) 321, 353, 611, 643
AseI ATTAAT 2 cut(s) 1934, 3180
AsiGI ACCGGT 1 cut(s) 6035
Asp700I GAANNNNTTC 4 cut(s) 1215, 2541, 2637, 4666
Asp718I GGTACC 1 cut(s) 6032
AspA2I CCTAGG 1 cut(s) 3995
AspLEI GCGC 3 cut(s) 2993, 3043, 5587
AspS9I GGNCC 8 cut(s) 319, 608, 2012, 3052, 3713, 4030, 4325, 4367
AsuC2I CCSGG 1 cut(s) 674
AsuII TTCGAA 2 cut(s) 3254, 6207
AvaI CYCGRG 2 cut(s) 4984, 5859
AvaII GGWCC 3 cut(s) 2012, 3052, 3713
AvrII CCTAGG 1 cut(s) 3995
AxyI CCTNAGG 1 cut(s) 3424
BaeI ACNNNNGTAYC 6 cut(s) 2860, 2893, 5640, 5673, 6024, 6057
BalI TGGCCA 2 cut(s) 2059, 6103
BamHI GGATCC 1 cut(s) 5450
BanI GGYRCC 3 cut(s) 1054, 4007, 6032
BanII GRGCYC 2 cut(s) 4772, 5673
BauI CACGAG 4 cut(s) 33, 268, 2694, 3036
BbrPI CACGTG 2 cut(s) 5031, 5624
BbsI GAAGAC 6 cut(s) 1155, 1362, 1473, 1851, 4437, 5264
Bbv12I GWGCWC 2 cut(s) 4772, 5673
BbvCI CCTCAGC 1 cut(s) 2313
BceAI ACGGC 3 cut(s) 1553, 4521, 6104
BcgI CGANNNNNNTGC 6 cut(s) 2273, 2307, 5558, 5592, 5885, 5919
BciT130I CCWGG 9 cut(s) 210, 303, 1547, 2774, 2914, 4653, 5315, 5870, 6437
BciVI GTATCC 3 cut(s) 1026, 2728, 5638
BclI TGATCA 2 cut(s) 3784, 6051
BcnI CCSGG 1 cut(s) 674
BcoDI GTCTC 8 cut(s) 1288, 1735, 2214, 2691, 3466, 3840, 3985, 4103
BcuI ACTAGT 4 cut(s) 4634, 5630, 6424, 6569
BfoI RGCGCY 2 cut(s) 3044, 5588
BfrI CTTAAG 2 cut(s) 4045, 5729
BfuAI ACCTGC 3 cut(s) 3355, 4601, 5941
BfuI GTATCC 3 cut(s) 1026, 2728, 5638
BglII AGATCT 1 cut(s) 6080
BlnI CCTAGG 1 cut(s) 3995
BlpI GCTNAGC 1 cut(s) 5457
BmcAI AGTACT 3 cut(s) 1744, 4093, 4889
Bme18I GGWCC 3 cut(s) 2012, 3052, 3713
BmeT110I CYCGRG 2 cut(s) 4984, 5859
BmgBI CACGTC 2 cut(s) 402, 4793
BmgT120I GGNCC 8 cut(s) 319, 608, 2012, 3052, 3713, 4030, 4325, 4367
BmrI ACTGGG 1 cut(s) 74
BmuI ACTGGG 1 cut(s) 74
BoxI GACNNNNGTC 1 cut(s) 1540
BpiI GAAGAC 6 cut(s) 1155, 1362, 1473, 1851, 4437, 5264
BplI GAGNNNNNCTC 2 cut(s) 43, 75
BpmI CTGGAG 1 cut(s) 2433
Bpu10I CCTNAGC 1 cut(s) 2313
Bpu1102I GCTNAGC 1 cut(s) 5457
Bpu14I TTCGAA 2 cut(s) 3254, 6207
BpuEI CTTGAG 3 cut(s) 2537, 2824, 5687
BpuMI CCSGG 1 cut(s) 674
Bsa29I ATCGAT 2 cut(s) 9, 1953
BsaAI YACGTR 3 cut(s) 5031, 5624, 5656
BsaBI GATNNNNATC 1 cut(s) 5287
BsaHI GRCGYC 2 cut(s) 1152, 1191
BsaI GGTCTC 1 cut(s) 3985
BsaWI WCCGGW 2 cut(s) 3116, 6035
Bse118I RCCGGY 4 cut(s) 230, 488, 4032, 6035
Bse21I CCTNAGG 1 cut(s) 3424
Bse3DI GCAATG 5 cut(s) 2004, 2126, 3065, 5646, 6618
Bse8I GATNNNNATC 1 cut(s) 5287
BseBI CCWGG 9 cut(s) 210, 303, 1547, 2774, 2914, 4653, 5315, 5870, 6437
BseCI ATCGAT 2 cut(s) 9, 1953
BseJI GATNNNNATC 1 cut(s) 5287
BseMI GCAATG 5 cut(s) 2004, 2126, 3065, 5646, 6618
BseMII CTCAG 9 cut(s) 89, 2033, 2304, 3369, 3435, 3438, 3963, 4480, 5471
BseRI GAGGAG 1 cut(s) 602
BseYI CCCAGC 2 cut(s) 1693, 4715
BsgI GTGCAG 7 cut(s) 582, 793, 1587, 2570, 3594, 4136, 6069
Bsh1236I CGCG 5 cut(s) 198, 258, 2037, 2991, 2993
BshNI GGYRCC 3 cut(s) 1054, 4007, 6032
BshTI ACCGGT 1 cut(s) 6035
BshVI ATCGAT 2 cut(s) 9, 1953
BsiHKAI GWGCWC 2 cut(s) 4772, 5673
BsiHKCI CYCGRG 2 cut(s) 4984, 5859
BsiSI CCGG 6 cut(s) 231, 489, 673, 3117, 4033, 6036
BslFI GGGAC 6 cut(s) 384, 1528, 1998, 2791, 2974, 4889
BsmAI GTCTC 8 cut(s) 1288, 1735, 2214, 2691, 3466, 3840, 3985, 4103
BsmBI CGTCTC 1 cut(s) 3840
BsmFI GGGAC 6 cut(s) 384, 1528, 1998, 2791, 2974, 4889
Bso31I GGTCTC 1 cut(s) 3985
BsoBI CYCGRG 2 cut(s) 4984, 5859
Bsp119I TTCGAA 2 cut(s) 3254, 6207
Bsp1286I GDGCHC 2 cut(s) 4772, 5673
Bsp1407I TGTACA 3 cut(s) 4193, 6463, 6546
Bsp1720I GCTNAGC 1 cut(s) 5457
Bsp19I CCATGG 2 cut(s) 1849, 2016
BspCNI CTCAG 9 cut(s) 88, 2034, 2305, 3370, 3436, 3437, 3964, 4479, 5470
BspDI ATCGAT 2 cut(s) 9, 1953
BspFNI CGCG 5 cut(s) 198, 258, 2037, 2991, 2993
BspHI TCATGA 3 cut(s) 466, 4039, 6574
BspMAI CTGCAG 7 cut(s) 706, 2860, 3366, 4375, 4705, 5833, 5990
BspMI ACCTGC 3 cut(s) 3355, 4601, 5941
BspQI GCTCTTC 1 cut(s) 3597
BspT104I TTCGAA 2 cut(s) 3254, 6207
BspT107I GGYRCC 3 cut(s) 1054, 4007, 6032
BspTI CTTAAG 2 cut(s) 4045, 5729
BspTNI GGTCTC 1 cut(s) 3985
BsrBI CCGCTC 1 cut(s) 5456
BsrDI GCAATG 5 cut(s) 2004, 2126, 3065, 5646, 6618
BsrFI RCCGGY 4 cut(s) 230, 488, 4032, 6035
BsrGI TGTACA 3 cut(s) 4193, 6463, 6546
BssAI RCCGGY 4 cut(s) 230, 488, 4032, 6035
BssNAI GTATAC 1 cut(s) 5816
BssNI GRCGYC 2 cut(s) 1152, 1191
BssSI CACGAG 4 cut(s) 33, 268, 2694, 3036
BssT1I CCWWGG 6 cut(s) 535, 1849, 2016, 2442, 3995, 6098
Bst1107I GTATAC 1 cut(s) 5816
Bst2BI CACGAG 4 cut(s) 33, 268, 2694, 3036
Bst2UI CCWGG 9 cut(s) 210, 303, 1547, 2774, 2914, 4653, 5315, 5870, 6437
Bst6I CTCTTC 7 cut(s) 1775, 2694, 3597, 4138, 4276, 5049, 6126
BstACI GRCGYC 2 cut(s) 1152, 1191
BstAFI CTTAAG 2 cut(s) 4045, 5729
BstAPI GCANNNNNTGC 5 cut(s) 402, 1565, 2557, 4414, 4709
BstAUI TGTACA 3 cut(s) 4193, 6463, 6546
BstBAI YACGTR 3 cut(s) 5031, 5624, 5656
BstBI TTCGAA 2 cut(s) 3254, 6207
BstDSI CCRYGG 3 cut(s) 603, 1849, 2016
BstEII GGTNACC 2 cut(s) 1165, 3119
BstENI CCTNNNNNAGG 1 cut(s) 584
BstFNI CGCG 5 cut(s) 198, 258, 2037, 2991, 2993
BstH2I RGCGCY 2 cut(s) 3044, 5588
BstHHI GCGC 3 cut(s) 2993, 3043, 5587
BstMAI GTCTC 8 cut(s) 1288, 1735, 2214, 2691, 3466, 3840, 3985, 4103
BstNI CCWGG 9 cut(s) 210, 303, 1547, 2774, 2914, 4653, 5315, 5870, 6437
BstNSI RCATGY 8 cut(s) 1526, 1563, 1593, 3758, 4412, 4631, 5168, 5324
BstPAI GACNNNNGTC 1 cut(s) 1540
BstPI GGTNACC 2 cut(s) 1165, 3119
BstSNI TACGTA 1 cut(s) 5656
BstUI CGCG 5 cut(s) 198, 258, 2037, 2991, 2993
BstV2I GAAGAC 6 cut(s) 1155, 1362, 1473, 1851, 4437, 5264
BstX2I RGATCY 8 cut(s) 1982, 2845, 4288, 4501, 4841, 5450, 5872, 6080
BstXI CCANNNNNNTGG 2 cut(s) 2258, 3723
BstYI RGATCY 8 cut(s) 1982, 2845, 4288, 4501, 4841, 5450, 5872, 6080
BstZ17I GTATAC 1 cut(s) 5816
Bsu15I ATCGAT 2 cut(s) 9, 1953
Bsu36I CCTNAGG 1 cut(s) 3424
BsuI GTATCC 3 cut(s) 1026, 2728, 5638
BsuTUI ATCGAT 2 cut(s) 9, 1953
BtgI CCRYGG 3 cut(s) 603, 1849, 2016
BtgZI GCGATG 3 cut(s) 2099, 2638, 6491
BtrI CACGTC 2 cut(s) 402, 4793
BtsI GCAGTG 4 cut(s) 781, 1575, 5818, 6021
BveI ACCTGC 3 cut(s) 3355, 4601, 5941
CaiI CAGNNNCTG 7 cut(s) 1985, 2177, 2744, 3437, 3560, 3971, 4844
CciI TCATGA 3 cut(s) 466, 4039, 6574
CfoI GCGC 3 cut(s) 2993, 3043, 5587
Cfr10I RCCGGY 4 cut(s) 230, 488, 4032, 6035
Cfr13I GGNCC 8 cut(s) 319, 608, 2012, 3052, 3713, 4030, 4325, 4367
ClaI ATCGAT 2 cut(s) 9, 1953
CseI GACGC 4 cut(s) 325, 1160, 5645, 5840
CspAI ACCGGT 1 cut(s) 6035
CspCI CAANNNNNGTGG 4 cut(s) 585, 620, 6150, 6185
EaeI YGGCCR 2 cut(s) 2057, 6101
Eam1104I CTCTTC 7 cut(s) 1775, 2694, 3597, 4138, 4276, 5049, 6126
EarI CTCTTC 7 cut(s) 1775, 2694, 3597, 4138, 4276, 5049, 6126
EciI GGCGGA 1 cut(s) 2350
Ecl136II GAGCTC 2 cut(s) 4770, 5671
Eco105I TACGTA 1 cut(s) 5656
Eco130I CCWWGG 6 cut(s) 535, 1849, 2016, 2442, 3995, 6098
Eco147I AGGCCT 1 cut(s) 1344
Eco24I GRGCYC 2 cut(s) 4772, 5673
Eco31I GGTCTC 1 cut(s) 3985
Eco32I GATATC 2 cut(s) 7, 4836
Eco47I GGWCC 3 cut(s) 2012, 3052, 3713
Eco53kI GAGCTC 2 cut(s) 4770, 5671
Eco72I CACGTG 2 cut(s) 5031, 5624
Eco81I CCTNAGG 1 cut(s) 3424
Eco88I CYCGRG 2 cut(s) 4984, 5859
Eco91I GGTNACC 2 cut(s) 1165, 3119
EcoICRI GAGCTC 2 cut(s) 4770, 5671
EcoNI CCTNNNNNAGG 1 cut(s) 584
EcoO109I RGGNCCY 3 cut(s) 319, 608, 3052
EcoO65I GGTNACC 2 cut(s) 1165, 3119
EcoRI GAATTC 3 cut(s) 3643, 5038, 6291
EcoRII CCWGG 9 cut(s) 208, 301, 1545, 2772, 2912, 4651, 5313, 5868, 6435
EcoRV GATATC 2 cut(s) 7, 4836
EcoT14I CCWWGG 6 cut(s) 535, 1849, 2016, 2442, 3995, 6098
EcoT22I ATGCAT 1 cut(s) 1377
EcoT38I GRGCYC 2 cut(s) 4772, 5673
ErhI CCWWGG 6 cut(s) 535, 1849, 2016, 2442, 3995, 6098
Esp3I CGTCTC 1 cut(s) 3840
FalI AAGNNNNNCTT 2 cut(s) 933, 965
FaqI GGGAC 6 cut(s) 384, 1528, 1998, 2791, 2974, 4889
FauI CCCGC 1 cut(s) 339
FauNDI CATATG 3 cut(s) 1371, 2071, 4354
FbaI TGATCA 2 cut(s) 3784, 6051
FblI GTMKAC 4 cut(s) 3744, 4540, 5815, 5854
FriOI GRGCYC 2 cut(s) 4772, 5673
GlaI GCGC 3 cut(s) 2992, 3042, 5586
GsaI CCCAGC 2 cut(s) 1697, 4719
GsuI CTGGAG 1 cut(s) 2433
HaeII RGCGCY 2 cut(s) 3044, 5588
HapII CCGG 6 cut(s) 231, 489, 673, 3117, 4033, 6036
HgaI GACGC 4 cut(s) 325, 1160, 5645, 5840
HhaI GCGC 3 cut(s) 2993, 3043, 5587
Hin1I GRCGYC 2 cut(s) 1152, 1191
Hin6I GCGC 3 cut(s) 2991, 3041, 5585
HinP1I GCGC 3 cut(s) 2991, 3041, 5585
HincII GTYRAC 9 cut(s) 823, 1659, 1900, 2607, 3209, 4541, 6276, 6301, 6531
HindII GTYRAC 9 cut(s) 823, 1659, 1900, 2607, 3209, 4541, 6276, 6301, 6531
HindIII AAGCTT 3 cut(s) 245, 3214, 5432
HpaI GTTAAC 4 cut(s) 1659, 2607, 6301, 6531
HpaII CCGG 6 cut(s) 231, 489, 673, 3117, 4033, 6036
Hpy99I CGWCG 3 cut(s) 341, 1636, 4542
HpyCH4IV ACGT 9 cut(s) 401, 1191, 1601, 2875, 4792, 5030, 5623, 5655, 6181
HpySE526I ACGT 9 cut(s) 401, 1191, 1601, 2875, 4792, 5030, 5623, 5655, 6181
Hsp92I GRCGYC 2 cut(s) 1152, 1191
HspAI GCGC 3 cut(s) 2991, 3041, 5585
KpnI GGTACC 1 cut(s) 6036
Ksp22I TGATCA 2 cut(s) 3784, 6051
KspAI GTTAAC 4 cut(s) 1659, 2607, 6301, 6531
LguI GCTCTTC 1 cut(s) 3597
MaeII ACGT 9 cut(s) 401, 1191, 1601, 2875, 4792, 5030, 5623, 5655, 6181
MbiI CCGCTC 1 cut(s) 5456
MfeI CAATTG 1 cut(s) 2675
MflI RGATCY 8 cut(s) 1982, 2845, 4288, 4501, 4841, 5450, 5872, 6080
MhlI GDGCHC 2 cut(s) 4772, 5673
MlsI TGGCCA 2 cut(s) 2059, 6103
MluI ACGCGT 1 cut(s) 2035
MluNI TGGCCA 2 cut(s) 2059, 6103
MlyI GAGTC 3 cut(s) 1240, 3041, 3183
MmeI TCCRAC 6 cut(s) 440, 2076, 2373, 2813, 3837, 5592
Mox20I TGGCCA 2 cut(s) 2059, 6103
Mph1103I ATGCAT 1 cut(s) 1377
MroXI GAANNNNTTC 4 cut(s) 1215, 2541, 2637, 4666
MscI TGGCCA 2 cut(s) 2059, 6103
Msp20I TGGCCA 2 cut(s) 2059, 6103
MspA1I CMGCKG 6 cut(s) 346, 1021, 2453, 4715, 5884, 5899
MspCI CTTAAG 2 cut(s) 4045, 5729
MspI CCGG 6 cut(s) 231, 489, 673, 3117, 4033, 6036
MunI CAATTG 1 cut(s) 2675
MvaI CCWGG 9 cut(s) 210, 303, 1547, 2774, 2914, 4653, 5315, 5870, 6437
MvnI CGCG 5 cut(s) 198, 258, 2037, 2991, 2993
NciI CCSGG 1 cut(s) 674
NcoI CCATGG 2 cut(s) 1849, 2016
NdeI CATATG 3 cut(s) 1371, 2071, 4354
NmeAIII GCCGAG 2 cut(s) 1458, 3529
NsiI ATGCAT 1 cut(s) 1377
NspI RCATGY 8 cut(s) 1526, 1563, 1593, 3758, 4412, 4631, 5168, 5324
NspV TTCGAA 2 cut(s) 3254, 6207
OliI CACNNNNGTG 1 cut(s) 2256
PacI TTAATTAA 1 cut(s) 4884
PaeI GCATGC 1 cut(s) 4412
PaeR7I CTCGAG 1 cut(s) 4984
PagI TCATGA 3 cut(s) 466, 4039, 6574
PaqCI CACCTGC 1 cut(s) 4601
PceI AGGCCT 1 cut(s) 1344
PciI ACATGT 2 cut(s) 4627, 5164
PciSI GCTCTTC 1 cut(s) 3597
PdmI GAANNNNTTC 4 cut(s) 1215, 2541, 2637, 4666
PflFI GACNNNGTC 1 cut(s) 4850
PinAI ACCGGT 1 cut(s) 6035
PleI GAGTC 3 cut(s) 1239, 3040, 3183
PmaCI CACGTG 2 cut(s) 5031, 5624
PmlI CACGTG 2 cut(s) 5031, 5624
PpsI GAGTC 3 cut(s) 1239, 3040, 3183
Ppu21I YACGTR 3 cut(s) 5031, 5624, 5656
PpuMI RGGWCCY 1 cut(s) 3052
PscI ACATGT 2 cut(s) 4627, 5164
PshAI GACNNNNGTC 1 cut(s) 1540
PshBI ATTAAT 2 cut(s) 1934, 3180
PsiI TTATAA 2 cut(s) 5496, 6263
Psp124BI GAGCTC 2 cut(s) 4772, 5673
Psp1406I AACGTT 1 cut(s) 6181
Psp5II RGGWCCY 1 cut(s) 3052
Psp6I CCWGG 9 cut(s) 208, 301, 1545, 2772, 2912, 4651, 5313, 5868, 6435
PspCI CACGTG 2 cut(s) 5031, 5624
PspEI GGTNACC 2 cut(s) 1165, 3119
PspFI CCCAGC 2 cut(s) 1693, 4715
PspGI CCWGG 9 cut(s) 208, 301, 1545, 2772, 2912, 4651, 5313, 5868, 6435
PspPI GGNCC 8 cut(s) 319, 608, 2012, 3052, 3713, 4030, 4325, 4367
PspPPI RGGWCCY 1 cut(s) 3052
PspXI VCTCGAGB 1 cut(s) 4984
PsrI GAACNNNNNNTAC 2 cut(s) 174, 206
PstI CTGCAG 7 cut(s) 706, 2860, 3366, 4375, 4705, 5833, 5990
PstNI CAGNNNCTG 7 cut(s) 1985, 2177, 2744, 3437, 3560, 3971, 4844
PsuI RGATCY 8 cut(s) 1982, 2845, 4288, 4501, 4841, 5450, 5872, 6080
PsyI GACNNNGTC 1 cut(s) 4850
PvuII CAGCTG 2 cut(s) 2453, 4715
SacI GAGCTC 2 cut(s) 4772, 5673
SalI GTCGAC 1 cut(s) 4539
SapI GCTCTTC 1 cut(s) 3597
Sau96I GGNCC 8 cut(s) 319, 608, 2012, 3052, 3713, 4030, 4325, 4367
ScaI AGTACT 3 cut(s) 1744, 4093, 4889
SchI GAGTC 3 cut(s) 1240, 3041, 3183
SduI GDGCHC 2 cut(s) 4772, 5673
Sfr274I CTCGAG 1 cut(s) 4984
SfuI TTCGAA 2 cut(s) 3254, 6207
SinI GGWCC 3 cut(s) 2012, 3052, 3713
SlaI CTCGAG 1 cut(s) 4984
SmlI CTYRAG 6 cut(s) 2516, 2839, 4045, 4984, 5702, 5729
SmoI CTYRAG 6 cut(s) 2516, 2839, 4045, 4984, 5702, 5729
SnaBI TACGTA 1 cut(s) 5656
SpeI ACTAGT 4 cut(s) 4634, 5630, 6424, 6569
SphI GCATGC 1 cut(s) 4412
SseBI AGGCCT 1 cut(s) 1344
SspI AATATT 4 cut(s) 2236, 5465, 5500, 6349
SstI GAGCTC 2 cut(s) 4772, 5673
StuI AGGCCT 1 cut(s) 1344
StyI CCWWGG 6 cut(s) 535, 1849, 2016, 2442, 3995, 6098
TaiI ACGT 9 cut(s) 404, 1194, 1604, 2878, 4795, 5033, 5626, 5658, 6184
TatI WGTACW 9 cut(s) 1742, 2820, 3902, 4091, 4128, 4193, 4887, 6463, 6546
TspGWI ACGGA 2 cut(s) 2870, 3730
Tth111I GACNNNGTC 1 cut(s) 4850
Vha464I CTTAAG 2 cut(s) 4045, 5729
VpaK11BI GGWCC 3 cut(s) 2012, 3052, 3713
VspI ATTAAT 2 cut(s) 1934, 3180
XagI CCTNNNNNAGG 1 cut(s) 584
XbaI TCTAGA 2 cut(s) 4015, 4271
XceI RCATGY 8 cut(s) 1526, 1563, 1593, 3758, 4412, 4631, 5168, 5324
XcmI CCANNNNNNNNNTGG 1 cut(s) 4869
XhoI CTCGAG 1 cut(s) 4984
XmaJI CCTAGG 1 cut(s) 3995
XmiI GTMKAC 4 cut(s) 3744, 4540, 5815, 5854
XmnI GAANNNNTTC 4 cut(s) 1215, 2541, 2637, 4666
ZraI GACGTC 1 cut(s) 1192
ZrmI AGTACT 3 cut(s) 1744, 4093, 4889
Zsp2I ATGCAT 1 cut(s) 1377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.