MD12G1142100.v1.1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0007182
Physical Location & Seq
Reverse (-)
22031130 .. 22049405
18276 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1142100.v1.1.491

Sequence Viewer

Length: 6237 bp
ATGGAAGTCGATTTGCCTCCTGAATCCATCCACATCAAGCATCGTGATCGAATCGTGCGGAGGCTTGCTCAATTGGGAGTGCCAAACGAATTTCTTGGTGAATATCAACGAGGGTTGATTGCTTATGTTAAGAAAAACAAGCACCGGATACCGGAACTGGTTTCCGCTGTTCTACCTGCTGATGAGGAAGTGGCAGAAGTTTTAAATGAAGCTAAACCAGGGGCTAAGAAACAAGGTGCAAGCAGGAAAAATAGATTCCGTGAAAACATGTCGTGGTTGCAGTGGTTGATGTTTGAGGGCGAACCTGAGGCAGCCCTGAGGAGTCTCTCCAAAATGAGTGTTGGGCAGCGTGGTTTTTGCGGAGCTGTTTGGGGACAGAACGATATTGCATACAGATGCCGAACTTGTGAAAATGATCCAACCTGTGCAATTTGTGTTCCGTGTTTTCAGAATGGGAACCACAAGGATCATGATTATTCAGTTATTTATACTGGTGGTGGTTGTTGTGATTGTGGTGATGTTACAGCATGGAAGCGCGAAGGCTTTTGTTCAAAGCATAAAGGTGTGGAACAGATACAACCTCTCCCGAAGGAGATTGCAAACATCGTAGGGCCTGTTCTTGATTGCCTGTTTGATATATGGAAAACCAAGCTGGTTCTTGGTGAAACTATCTGGCGGGAAAATGTTAGAGCAAGTGACCAAGTTGGAGGGCATAGAAAGGTTGCAAATGAGTTAACTTTTGTCGTGGTTGAGATGCTTCTAGAATTCTGTAAGTACAGTGAGAGTCTGCTAAGTTCTGTATCCAAGACGGTTTTAGCATCAGGGGATCTATTGGGGATTCTTGTGAGAGCAGAGAGGTTTTTGAATGACACTGTTACGAAAAGGCTGCATGAGTTGCTACTGAAACTGCTGGGAGAACCAGTTTTCAAATTTGAGTTTGCCAAAGTATTTCTGAGTTATTACCCAGCCGTTGTAAGTGAAGCCATAAAGGGATTAAGTGACCATTCTTCGAAAAAGTATCCACTGCTCTCTGTATTCTCTGTACAAATCTTCACGGTGCCAACTTTAACACCTCGTCTTGTTAAAGAAATGAACCTACTGTCTATGCTGATGGGATGCTTGGAAGATATTTTCATTTCCTGTGCTGGGGATGACGGTCGTTTACAGGTTACCACGTTGTCAAATTTGTATGAGATCACATTTCGTGTGATTGAAGATATTCGATTTGTTATGAGCCATGCTACAGTGCCCAAATACGTAACACGTGACCAGCAAGATATTTCAAGAACTTGGACACGACTCTTGGCTTTCATTCAAGGGATGAACCCACAGAAAAGAGAAACAGGCTTACACATTGAAGAAGAAAATGAGAATATGCATCTGCCATTTGTTCTAGGTCATTCAATTGCCAACATTCATTCTTTATTGGTGGATGGGGCATTTTCTGTGGCTATTGACAAGATGGATGAAGACTCTCTTTTCAGAACATATAAGCAGGATATGGATGACATGGATAGCCTAAGGCATTCAGAAGTAGGATGGCTGTCCCAGGAAAGTTCTGCATGCAGTGCAGTAGGGAGTAGTAGTTCATTTGCGTGCGAGTCGAAGGTTTCTGAAGATAAATCGGATGCTCTTTCTGATTTGTTGATTCCACCTTCTGTGATGTGGCTAACATATGAGTGCTTGAGGGCAATTAAGAATTGGCTGGGAGTTGATAATACCTCCAGGACTCTTCTTGATGCCTCTTCTCCAAGTACTAGCAATTTCTCTGTTAGCAATTTTTCAGCATTGAAGAGAACATTATCCAAGATCAGGAAAGGAAAATATATTTTTGGTAGACTTGGCAGTTCAAGTGAAGATCATGGCAAGCAGCATTCATCACATGGACATAGTGATTGCAATATGAGTATTGACACCCAAAATGGCAAAAGTGCAGGGCAGGAGAGTAAATTGATGGTTACTGACGAAATTGATTCAGTTAATGTCTGCAATCCTGCAGGATTTGATGATAGTGCTATGGAAGTAGATGGTGCCATGGACTTGGATGCTCAACGCGTTCTGAATTTGTCTGATTGGCCAGACATAACATATGATGTTAGTTCGCAGGATATATCTGTTCACATTCCTTTACACCGTTTACTTTCCTTGCTTTTACAGAAAGCATTAAGAAGATGTTTCGGTGAAGTGCCAGATCTGGCTAGCGTTACTTCTGCAAATTCATCATCAGCTATTTTCACTGACTTCTTTGGAAATATTCTGGGAGGCTGCCACCCATATGGATTTTCTGCTTTTGTTATGGAGCATCCCCTTCGGATCAGAGTATTTTGTGCTGAGGTTCATGCTGGAATGTGGCGGAAAAATGGGGATGCCGCCCTGTTACCTTGTGAGTGGTATCGTTCAGTTCGCTGGTCTGAACAGGGTTTGGAGCTTGATCTGTTTCTGCTTCAGTGCTGTGCTGCCTTAGCTCCAGCTGATCCTTATGTTAACAGAATTATAGAACGCTTTGGCCTGTCCAGCTACCTTTCTTTGAACCTTGAGCGCTCTAGTGAATATGAAACTATTCTAGTGCAAGAAATGCTCACTCTTATTATACAAATAATCAAAGAAAGGCGATTTTGTGGTCTAACTAAAGCTGAAAGTTTGAAAAGAGAGTTGGTTCATAAATTAGCCATTGCAGATGGCACTCATAGTCAATTGGTGAAATCTCTTCCTCGTGATCTCTCCAAGTCTGATCAGCTACATGGAATTTTGGAATCCATTGCCGTGTACTCCAATCCCTCTGGCTTTAATCAGGGGACTTATTCGCTACAATGGACATTTTGGAAAGAACTGGATTTGTACTACTCTCGTTGGAACTCGAGGGATCTGCAAGCTGCAGAAGAAAGATACTTGCGTTTTCGTGGTGTTTGTGCATTGACCACTCAGCTACCCAGATGGACTAAGATTTATTCTCCTTTCAAAGGGGTAGCTAGAATAGCTACTTGTAAATCAGTGCTTCAAATTATCCGTGCTGTGCTGTTTTATGCTGTTTTTTCTGATAAATCAAATGATTCACGAGCTCCTGATAGTGTTCTCTTAACTGCATTGCACTTACTCTCATTAGCATTAGACATTTGTTCTCAACATAAGGAAGCTGGTGATAAGTCTTGTTATGATGGAGATGCAATTCCAATGCTAGCTTTTGCTGGGGAAGAAATTACTGAAGGGCGATACTTTGGTGCTGGTCAACAAAGCTTACTGTCACTTCTTGTTATCTCGATGAGGATGTATAAGAAAGAGAATGTCAACAATTGTTTGGAAGCTGGCAGTTGCGACCTCTCTTCTTTAATTGGAAGCTTATTAAAGAAGTTTGTAGAGATTGATTCCGGATGTATGACCATATTACAACAACTTGCACCTGAAGTGATTGGTCACGTATCACAATCTTCTTTAAATGGTGATGCTAAGACCTCAGGTTCAATTTCTTACTCTGAGAAGCGCAAGGCAAAAGCTAGAGAGAGACAGGCTGCCATATTGGAAAAAATGAGAGCCGAACAATCTAAGTTTATGGCCAGTGTAAATTCCACTGTGGATGAGGGTTCAAAATCTGAGCAAGAGGTTTGTAAACTCAATATTGAAGATGACTTGGAAGAATCTGAACAAGTTGTTTGCTCTCTTTGTCATGATCCCAGTTCCAGAAATCCCATATCCTACTTGGTTTTACTTCAGAAATCTAGACTCTTAAGTTTCATAGAAAGAGGTCCCCGGTCATGGGAGCAACCACCTTGGACAGACAAGGAGCACATCTCTATTACCACAAACAAGGTGAGTGATCAATCTGGATCAAGCACTTTATCAAGAGGTTCAGGACCAGAACAGGTGCCATCTTATTCTTGCAGACAGTTGGTTCAGAATGCAATCACCAAATTTGCCTATCATGGACAACCTAGGGATGTTGCCGCTCTTATAAATTTTCTCAAGGGCCGGTTCCATGAATTAAGTGCTGATATCCAAGTGCCACGTGAATCAAATGATGATATGGTGAAGACTTTATTTACATTTGAGACAATGGAAGACGATATGTACATCTCCATCCAAAAGGAAATGCGTGATAAGATACTTCATTCTAATCCTTTAGAGGATAAGGGATTTTCAACTCCTGAAGGGGATCAAGAAGAAACTAAGCATGCTGAATTTATGTTACTTGGAACATATATAGCTGCTCTTTCTAGAGAGGCAACAGAGATTCCTTCATCTTCTGAAAGTGCTCCTAATGAAAGGGTGCCAGTAGATTCTTCTCGTCTTTCAGCGCGTGACGGATTTGGCCTGACAGATTGTGATGGAATTTATCTTTCTTCCTGTGGACATGCTGTGCATCAGGGATGCCTTGATCGTTATTTATTTTCGTTGAAGGAAAGGTATCTCAGAAGAATTGTTTTTGAAGGGGGACATATTGCAGATCCGGATAAGGGGGAGTTTCTCTGCCCTGTATGCCGTCGACTTGCAAATTCTGTCTTGCCCGCATTGCCAGGGTTTCTCCATGTCACCAAGGAGCCTGTGCATTCAGGTGTTAATTCATCGCATGCTACTGGTCCATCGGTCAAATCAGTTGACGAAATTAATTCTCTTCAGCTTCAACAAGGGTTGGCTCTCTTGCGATCTGCAGCAGAGGCATCTGGGAAGGTTGGAAGTCTTAAAGGTTTCCCCCTTCAGAGATGTGGAAGAACGACCTCAGACCTTGAACCAATTTTTCGATTGCTCAGTAAGATGTATTTTCCAACTAAACAGGATAAGATATCAGGATCTGCCAGGGTTAACCACCCAATGCTTATGTGGGACACAATTAAGTACTCTCTACTATCAACAGAAATTGCTGCTCGTTCTGATGGGAAATATGCAACACCAAGCTTTGGCCTTAATGCGTTGTATAAGGAACTTGAATCAAGTAGATTTATTTTGTCCTTGTTGCTGAAAATAGTCCAAAGCAGAAGAAAGAATTCTCTTCATGTCCTTCAGAGATTTATAGGAATTCAAAGCTTTACAGAATCTATTCTATTTGGAGTTTCCATAGACGTTCGCAATGAAACATGTGGACAAGGTGCTATGCTGCGTATCTTGGAATATGCTGATCTGGCAGTATCGTATCCTGATATTCAGTTCTGGAGGCGAGCTTCTGATCCTGTCCTTGCTCGCGATCCTTTTTCATCATTGATGTGGGTTCTTTTTTGTCTTCCATACCGATTTTTATCATGTGAAGATTCATTGTTGTCCCTAGTGCACTTATTTTATGTTGTCTCTGTAGTTCAGGGCATAATGACATATTGTGGGAAAAATCAATGTGACATTAGTGGATTAGGTATTGATGATTGCCTGGTTACTGACCTCTCTAAACTTATGGAAGAATCTGGAGCCACTCAGCAATTTTTTGTTTCAAATTATATTGGCTCTTCTCCTAATATCAAGAACATAGTTCGTAGCCTGAGTTTCCCTTATTTGCGGAGGTGTGCATTGCTGTTGAAGCTATTGAACTCCTGTTCCCGAGTACCATTCCATGAAAGGTATAATGTCTTGGATAGATCTCATGCTATTGGTGACATGATGGACACCACTTATGTCGCACTAGTGGAGCTAAATGATGTTCAGGAAATAGAAAGCAAGTTAAAGATTCCTTCTCTGGATGTTATCTTTAAGGATAATGTGGTACGTTCAATAACTCAGAAATGGTTCCATCATTTTCGGAAGGAGTTTGAAGTCCAAAGATTTCAGGGAAGCATGCACTGTATCCCTGCAGTTCCCTTCCAATTGATGCGTCTTCCCCAAGTTTACCAGGACCTATTGCAGAGGTACATAAAACAGCGTTGTCCTGATTGCAAAAATATCCTTGATGACCCTGCATTGTGCTTGCTTTGTGGGAGATTATGTTCTCCAAGCTGGAAGTCATGTTGCAGGGAAAGTGGATGCCAAACTCATGCAGTGTCCTGTGGTTCTGGTACTGGTGTATTTCTGTTGATCAGGAGGACAACAATTCTGCTGCAGAGGTCTGCACGGCAAGCTCCTTGGCCATCGCCCTACTTGGATGCTTTTGGAGAAGAGGATGTTGAAATGCACAGAGGAAAGCCACTGTACTTGAACGAGGAACGCTATGCAGCTTTAACTTACTTGGTTGCTTCTCATGGCCTTGATCGAAGCTCCAAGGTCCTTGGACAAACTACAATTGGTTCTTTCTTCATGGTGGTATATATGTTTGACGCAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000151 GO:0000280 GO:0001101 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006259 GO:0006310 GO:0006325 GO:0006342 GO:0006355 GO:0006464 GO:0006508 GO:0006511 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0007049 GO:0007127 GO:0007131 GO:0007140 GO:0007141 GO:0007276 GO:0007283 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009605 GO:0009607 GO:0009620 GO:0009719 GO:0009725 GO:0009737 GO:0009889 GO:0009890 GO:0009892 GO:0009894 GO:0009966 GO:0009968 GO:0009987 GO:0010029 GO:0010033 GO:0010243 GO:0010468 GO:0010498 GO:0010528 GO:0010529 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010646 GO:0010648 GO:0016043 GO:0016458 GO:0016567 GO:0016569 GO:0016570 GO:0016574 GO:0016597 GO:0016740 GO:0019216 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0019941 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0023051 GO:0023057 GO:0030163 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031406 GO:0032006 GO:0032007 GO:0032446 GO:0032501 GO:0032504 GO:0032991 GO:0033522 GO:0033993 GO:0034641 GO:0035825 GO:0036094 GO:0036211 GO:0040029 GO:0042221 GO:0043161 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043177 GO:0043200 GO:0043201 GO:0043207 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0044703 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048232 GO:0048285 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0048609 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0050994 GO:0051171 GO:0051172 GO:0051239 GO:0051252 GO:0051253 GO:0051276 GO:0051321 GO:0051603 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0061630 GO:0061659 GO:0061982 GO:0065007 GO:0070647 GO:0070728 GO:0070887 GO:0071229 GO:0071230 GO:0071233 GO:0071310 GO:0071417 GO:0071495 GO:0071596 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097305 GO:0098542 GO:0140013 GO:0140096 GO:1900140 GO:1901360 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902494 GO:1902531 GO:1902532 GO:1902679 GO:1903046 GO:1903506 GO:1903507 GO:1990234 GO:2000026 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-UBR

Protein Analysis

2079

Amino Acids

232.94

Weight (kDa)

6.25

Isoelectric Point (pI)

48.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-UBR PF02207 120 - 186 3e-20 Putative zinc finger in N-recognin (UBR box)
WHD_UBR1 PF22960 881 - 965 2.7e-19 E3 ubiquitin-protein ligase UBR1-like, winged-helix domain
PRT6_C PF18995 1582 - 2049 2.1e-92 Proteolysis_6 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3947
Acc36I ACCTGC 1 cut(s) 184
AccB1I GGYRCC 4 cut(s) 1057, 2030, 3859, 4261
AccB7I CCANNNNNTGG 1 cut(s) 4889
AccBSI CCGCTC 1 cut(s) 3941
AccI GTMKAC 2 cut(s) 1837, 4477
AccII CGCG 4 cut(s) 537, 2055, 4291, 5172
AccIII TCCGGA 2 cut(s) 3365, 4441
AciI CCGC 9 cut(s) 58, 165, 360, 676, 2353, 2370, 3939, 4500, 5476
AcoI YGGCCR 3 cut(s) 2075, 3549, 6038
AcuI CTGAAG 9 cut(s) 1635, 2429, 3222, 3420, 3689, 4161, 4592, 4673, 4976
AcvI CACGTG 2 cut(s) 1265, 4001
AfeI AGCGCT 1 cut(s) 2540
AfiI CCNNNNNNNGG 8 cut(s) 151, 1146, 1812, 2960, 3750, 3751, 4448, 4889
AflII CTTAAG 1 cut(s) 3721
AflIII ACRYGT 4 cut(s) 267, 1262, 2053, 5066
AhlI ACTAGT 1 cut(s) 5599
AjnI CCWGG 7 cut(s) 217, 1548, 1724, 4507, 4787, 5349, 5805
AjuI GAANNNNNNNTTGG 2 cut(s) 2636, 2668
AloI GAACNNNNNNTCC 6 cut(s) 1570, 1602, 5498, 5530, 5884, 5916
Alw21I GWGCWC 4 cut(s) 3061, 3783, 4249, 5259
Alw26I GTCTC 4 cut(s) 329, 3493, 4037, 5278
Alw44I GTGCAC 1 cut(s) 5255
AlwNI CAGNNNCTG 1 cut(s) 4784
Ama87I CYCGRG 2 cut(s) 2857, 5517
Aor13HI TCCGGA 2 cut(s) 3365, 4441
Aor51HI AGCGCT 1 cut(s) 2540
AoxI GGCC 9 cut(s) 611, 2075, 2506, 3549, 3961, 4303, 4891, 6038, 6154
ApaLI GTGCAC 1 cut(s) 5255
AseI ATTAAT 1 cut(s) 4599
Asp700I GAANNNNTTC 5 cut(s) 1218, 2559, 4975, 5028, 5702
AspA2I CCTAGG 1 cut(s) 3926
AspLEI GCGC 4 cut(s) 537, 2541, 3480, 4291
AspS9I GGNCC 7 cut(s) 611, 3740, 3848, 3961, 4571, 5809, 6175
AsuC2I CCSGG 1 cut(s) 3745
AsuII TTCGAA 1 cut(s) 1010
AsuNHI GCTAGC 2 cut(s) 2198, 3175
AvaI CYCGRG 2 cut(s) 2857, 5517
AvaII GGWCC 5 cut(s) 3740, 3848, 4571, 5809, 6175
AvrII CCTAGG 1 cut(s) 3926
AxyI CCTNAGG 4 cut(s) 306, 317, 1520, 3451
BaeGI GKGCMC 2 cut(s) 1251, 5259
BaeI ACNNNNGTAYC 6 cut(s) 2878, 2911, 3202, 3235, 5961, 5994
BalI TGGCCA 3 cut(s) 2077, 3551, 6040
BanI GGYRCC 4 cut(s) 1057, 2030, 3859, 4261
BanII GRGCYC 1 cut(s) 3061
BauI CACGAG 2 cut(s) 2712, 3054
BbrPI CACGTG 2 cut(s) 1265, 4001
BbsI GAAGAC 5 cut(s) 1476, 4031, 4059, 5201, 5783
Bbv12I GWGCWC 4 cut(s) 3061, 3783, 4249, 5259
BbvCI CCTCAGC 1 cut(s) 2331
BceAI ACGGC 4 cut(s) 953, 2747, 4458, 6041
BcgI CGANNNNNNTGC 8 cut(s) 29, 63, 868, 902, 2291, 2325, 2848, 2882
BciT130I CCWGG 7 cut(s) 219, 1550, 1726, 4509, 4789, 5351, 5807
BciVI GTATCC 5 cut(s) 141, 811, 1029, 5133, 5771
BclI TGATCA 3 cut(s) 2731, 3811, 5988
BcnI CCSGG 1 cut(s) 3745
BcoDI GTCTC 4 cut(s) 329, 3493, 4037, 5278
BcuI ACTAGT 1 cut(s) 5599
BfmI CTRYAG 7 cut(s) 1242, 1995, 2874, 4641, 5277, 5766, 6011
BfoI RGCGCY 1 cut(s) 2542
BfrI CTTAAG 1 cut(s) 3721
BfuAI ACCTGC 1 cut(s) 184
BfuI GTATCC 5 cut(s) 141, 811, 1029, 5133, 5771
BglII AGATCT 2 cut(s) 2191, 5555
BlnI CCTAGG 1 cut(s) 3926
BmcAI AGTACT 2 cut(s) 1756, 4829
Bme1390I CCNGG 8 cut(s) 219, 1550, 1726, 3745, 4509, 4789, 5351, 5807
Bme18I GGWCC 5 cut(s) 3740, 3848, 4571, 5809, 6175
BmeT110I CYCGRG 2 cut(s) 2857, 5517
BmgT120I GGNCC 7 cut(s) 611, 3740, 3848, 3961, 4571, 5809, 6175
BmrFI CCNGG 8 cut(s) 219, 1550, 1726, 3745, 4509, 4789, 5351, 5807
BmrI ACTGGG 1 cut(s) 3663
BmtI GCTAGC 2 cut(s) 2202, 3179
BmuI ACTGGG 1 cut(s) 3663
BpiI GAAGAC 5 cut(s) 1476, 4031, 4059, 5201, 5783
BplI GAGNNNNNCTC 4 cut(s) 52, 84, 3770, 3802
BpmI CTGGAG 4 cut(s) 1708, 2451, 5161, 5406
Bpu10I CCTNAGC 2 cut(s) 2331, 2461
Bpu14I TTCGAA 1 cut(s) 1010
BpuEI CTTGAG 3 cut(s) 1705, 2555, 3941
BpuMI CCSGG 1 cut(s) 3745
BsaAI YACGTR 4 cut(s) 1258, 1265, 3415, 4001
BsaBI GATNNNNATC 1 cut(s) 5224
BsaWI WCCGGW 4 cut(s) 144, 151, 3365, 4441
BsaXI ACNNNNNCTCC 8 cut(s) 567, 597, 906, 936, 1570, 1600, 5884, 5914
Bsc4I CCNNNNNNNGG 8 cut(s) 151, 1146, 1812, 2960, 3750, 3751, 4448, 4889
Bse118I RCCGGY 1 cut(s) 3963
Bse1I ACTGG 9 cut(s) 162, 496, 920, 2835, 3552, 3669, 4265, 4573, 5977
Bse21I CCTNAGG 4 cut(s) 306, 317, 1520, 3451
Bse3DI GCAATG 6 cut(s) 2670, 2757, 3083, 4502, 5065, 5486
Bse8I GATNNNNATC 1 cut(s) 5224
BseAI TCCGGA 2 cut(s) 3365, 4441
BseBI CCWGG 7 cut(s) 219, 1550, 1726, 4509, 4789, 5351, 5807
BseJI GATNNNNATC 1 cut(s) 5224
BseLI CCNNNNNNNGG 8 cut(s) 151, 1146, 1812, 2960, 3750, 3751, 4448, 4889
BseMI GCAATG 6 cut(s) 2670, 2757, 3083, 4502, 5065, 5486
BseNI ACTGG 9 cut(s) 162, 496, 920, 2835, 3552, 3669, 4265, 4573, 5977
BseRI GAGGAG 1 cut(s) 334
BseSI GKGCMC 2 cut(s) 1251, 5259
BseYI CCCAGC 5 cut(s) 910, 964, 1145, 1705, 3185
BsgI GTGCAG 3 cut(s) 1590, 1953, 6006
Bsh1236I CGCG 4 cut(s) 537, 2055, 4291, 5172
Bsh1285I CGRYCG 1 cut(s) 1159
BshFI GGCC 9 cut(s) 613, 2077, 2508, 3551, 3963, 4305, 4893, 6040, 6156
BshNI GGYRCC 4 cut(s) 1057, 2030, 3859, 4261
BsiEI CGRYCG 1 cut(s) 1159
BsiHKAI GWGCWC 4 cut(s) 3061, 3783, 4249, 5259
BsiHKCI CYCGRG 2 cut(s) 2857, 5517
BsiSI CCGG 6 cut(s) 145, 152, 3366, 3745, 3964, 4442
BslFI GGGAC 7 cut(s) 387, 1531, 2809, 3726, 4440, 4829, 5233
BslI CCNNNNNNNGG 8 cut(s) 151, 1146, 1812, 2960, 3750, 3751, 4448, 4889
BsmAI GTCTC 4 cut(s) 329, 3493, 4037, 5278
BsmFI GGGAC 7 cut(s) 387, 1531, 2809, 3726, 4440, 4829, 5233
BsmI GAATGC 4 cut(s) 1525, 1873, 3898, 4540
BsnI GGCC 9 cut(s) 613, 2077, 2508, 3551, 3963, 4305, 4893, 6040, 6156
BsoBI CYCGRG 2 cut(s) 2857, 5517
Bsp119I TTCGAA 1 cut(s) 1010
Bsp1286I GDGCHC 5 cut(s) 1251, 3061, 3783, 4249, 5259
Bsp13I TCCGGA 2 cut(s) 3365, 4441
Bsp1407I TGTACA 2 cut(s) 1042, 4062
Bsp19I CCATGG 1 cut(s) 2034
Bsp68I TCGCGA 1 cut(s) 5172
BspACI CCGC 9 cut(s) 58, 165, 360, 676, 2353, 2370, 3939, 4500, 5476
BspANI GGCC 9 cut(s) 613, 2077, 2508, 3551, 3963, 4305, 4893, 6040, 6156
BspEI TCCGGA 2 cut(s) 3365, 4441
BspFNI CGCG 4 cut(s) 537, 2055, 4291, 5172
BspHI TCATGA 2 cut(s) 469, 3661
BspMAI CTGCAG 5 cut(s) 1999, 2878, 4645, 5770, 6015
BspMI ACCTGC 1 cut(s) 184
BspOI GCTAGC 2 cut(s) 2202, 3179
BspQI GCTCTTC 1 cut(s) 5431
BspT104I TTCGAA 1 cut(s) 1010
BspT107I GGYRCC 4 cut(s) 1057, 2030, 3859, 4261
BspTI CTTAAG 1 cut(s) 3721
BsrBI CCGCTC 1 cut(s) 3941
BsrDI GCAATG 6 cut(s) 2670, 2757, 3083, 4502, 5065, 5486
BsrFI RCCGGY 1 cut(s) 3963
BsrGI TGTACA 2 cut(s) 1042, 4062
BsrI ACTGG 9 cut(s) 162, 496, 920, 2835, 3552, 3669, 4265, 4573, 5977
BssAI RCCGGY 1 cut(s) 3963
BssSI CACGAG 2 cut(s) 2712, 3054
BssT1I CCWWGG 7 cut(s) 2034, 3764, 3926, 4527, 6035, 6171, 6178
Bst2BI CACGAG 2 cut(s) 2712, 3054
Bst2UI CCWGG 7 cut(s) 219, 1550, 1726, 4509, 4789, 5351, 5807
Bst6I CTCTTC 9 cut(s) 1737, 1750, 1787, 2712, 3325, 4611, 4986, 5431, 6063
BstAFI CTTAAG 1 cut(s) 3721
BstAPI GCANNNNNTGC 3 cut(s) 895, 1568, 2575
BstAUI TGTACA 2 cut(s) 1042, 4062
BstBAI YACGTR 4 cut(s) 1258, 1265, 3415, 4001
BstBI TTCGAA 1 cut(s) 1010
BstDSI CCRYGG 1 cut(s) 2034
BstEII GGTNACC 1 cut(s) 1168
BstENI CCTNNNNNAGG 1 cut(s) 2958
BstFNI CGCG 4 cut(s) 537, 2055, 4291, 5172
BstH2I RGCGCY 1 cut(s) 2542
BstHHI GCGC 4 cut(s) 537, 2541, 3480, 4291
BstMAI GTCTC 4 cut(s) 329, 3493, 4037, 5278
BstMCI CGRYCG 1 cut(s) 1159
BstNI CCWGG 7 cut(s) 219, 1550, 1726, 4509, 4789, 5351, 5807
BstNSI RCATGY 7 cut(s) 271, 1566, 4169, 4349, 4565, 5070, 5755
BstPI GGTNACC 1 cut(s) 1168
BstSCI CCNGG 8 cut(s) 217, 1548, 1724, 3743, 4507, 4787, 5349, 5805
BstSFI CTRYAG 7 cut(s) 1242, 1995, 2874, 4641, 5277, 5766, 6011
BstSLI GKGCMC 2 cut(s) 1251, 5259
BstSNI TACGTA 1 cut(s) 1258
BstUI CGCG 4 cut(s) 537, 2055, 4291, 5172
BstV2I GAAGAC 5 cut(s) 1476, 4031, 4059, 5201, 5783
BstX2I RGATCY 6 cut(s) 826, 2191, 2863, 4438, 4781, 5555
BstXI CCANNNNNNTGG 2 cut(s) 2041, 2276
BstYI RGATCY 6 cut(s) 826, 2191, 2863, 4438, 4781, 5555
Bsu36I CCTNAGG 4 cut(s) 306, 317, 1520, 3451
BsuI GTATCC 5 cut(s) 141, 811, 1029, 5133, 5771
BsuRI GGCC 9 cut(s) 613, 2077, 2508, 3551, 3963, 4305, 4893, 6040, 6156
BtgI CCRYGG 1 cut(s) 2034
BtgZI GCGATG 2 cut(s) 4542, 6027
BtsI GCAGTG 4 cut(s) 287, 1022, 1573, 5958
BtuMI TCGCGA 1 cut(s) 5172
BveI ACCTGC 1 cut(s) 184
CaiI CAGNNNCTG 1 cut(s) 4784
CciI TCATGA 2 cut(s) 469, 3661
CfoI GCGC 4 cut(s) 537, 2541, 3480, 4291
Cfr10I RCCGGY 1 cut(s) 3963
Cfr13I GGNCC 7 cut(s) 611, 3740, 3848, 3961, 4571, 5809, 6175
CseI GACGC 1 cut(s) 5777
CspCI CAANNNNNGTGG 2 cut(s) 6087, 6122
DraI TTTAAA 2 cut(s) 204, 3432
EaeI YGGCCR 3 cut(s) 2075, 3549, 6038
Eam1104I CTCTTC 9 cut(s) 1737, 1750, 1787, 2712, 3325, 4611, 4986, 5431, 6063
EarI CTCTTC 9 cut(s) 1737, 1750, 1787, 2712, 3325, 4611, 4986, 5431, 6063
EciI GGCGGA 1 cut(s) 2368
Ecl136II GAGCTC 1 cut(s) 3059
Eco105I TACGTA 1 cut(s) 1258
Eco130I CCWWGG 7 cut(s) 2034, 3764, 3926, 4527, 6035, 6171, 6178
Eco24I GRGCYC 1 cut(s) 3061
Eco32I GATATC 2 cut(s) 3988, 4776
Eco47I GGWCC 5 cut(s) 3740, 3848, 4571, 5809, 6175
Eco47III AGCGCT 1 cut(s) 2540
Eco53kI GAGCTC 1 cut(s) 3059
Eco57I CTGAAG 9 cut(s) 1635, 2429, 3222, 3420, 3689, 4161, 4592, 4673, 4976
Eco72I CACGTG 2 cut(s) 1265, 4001
Eco81I CCTNAGG 4 cut(s) 306, 317, 1520, 3451
Eco88I CYCGRG 2 cut(s) 2857, 5517
Eco91I GGTNACC 1 cut(s) 1168
EcoICRI GAGCTC 1 cut(s) 3059
EcoNI CCTNNNNNAGG 1 cut(s) 2958
EcoO109I RGGNCCY 4 cut(s) 611, 3740, 5809, 6175
EcoO65I GGTNACC 1 cut(s) 1168
EcoRI GAATTC 3 cut(s) 764, 4975, 5007
EcoRII CCWGG 7 cut(s) 217, 1548, 1724, 4507, 4787, 5349, 5805
EcoRV GATATC 2 cut(s) 3988, 4776
EcoT14I CCWWGG 7 cut(s) 2034, 3764, 3926, 4527, 6035, 6171, 6178
EcoT22I ATGCAT 1 cut(s) 1380
EcoT38I GRGCYC 1 cut(s) 3061
ErhI CCWWGG 7 cut(s) 2034, 3764, 3926, 4527, 6035, 6171, 6178
FalI AAGNNNNNCTT 2 cut(s) 1461, 1493
FaqI GGGAC 7 cut(s) 387, 1531, 2809, 3726, 4440, 4829, 5233
FauI CCCGC 2 cut(s) 669, 4507
FauNDI CATATG 3 cut(s) 1675, 2089, 2275
FbaI TGATCA 3 cut(s) 2731, 3811, 5988
FblI GTMKAC 2 cut(s) 1837, 4477
FriOI GRGCYC 1 cut(s) 3061
GlaI GCGC 4 cut(s) 536, 2540, 3479, 4290
GsaI CCCAGC 5 cut(s) 914, 968, 1149, 1709, 3189
GsuI CTGGAG 4 cut(s) 1708, 2451, 5161, 5406
HaeII RGCGCY 1 cut(s) 2542
HaeIII GGCC 9 cut(s) 613, 2077, 2508, 3551, 3963, 4305, 4893, 6040, 6156
HapII CCGG 6 cut(s) 145, 152, 3366, 3745, 3964, 4442
HgaI GACGC 1 cut(s) 5777
HhaI GCGC 4 cut(s) 537, 2541, 3480, 4291
Hin6I GCGC 4 cut(s) 535, 2539, 3478, 4289
HinP1I GCGC 4 cut(s) 535, 2539, 3478, 4289
HincII GTYRAC 7 cut(s) 735, 2485, 3227, 3286, 4478, 4591, 4795
HindII GTYRAC 7 cut(s) 735, 2485, 3227, 3286, 4478, 4591, 4795
HindIII AAGCTT 4 cut(s) 3232, 3334, 4885, 5014
HpaI GTTAAC 3 cut(s) 735, 2485, 4795
HpaII CCGG 6 cut(s) 145, 152, 3366, 3745, 3964, 4442
Hpy99I CGWCG 1 cut(s) 4479
HpyCH4IV ACGT 7 cut(s) 1175, 1257, 1264, 3414, 4000, 5052, 5683
HpySE526I ACGT 7 cut(s) 1175, 1257, 1264, 3414, 4000, 5052, 5683
HspAI GCGC 4 cut(s) 535, 2539, 3478, 4289
Kpn2I TCCGGA 2 cut(s) 3365, 4441
Ksp22I TGATCA 3 cut(s) 2731, 3811, 5988
KspAI GTTAAC 3 cut(s) 735, 2485, 4795
LguI GCTCTTC 1 cut(s) 5431
MaeII ACGT 7 cut(s) 1175, 1257, 1264, 3414, 4000, 5052, 5683
MbiI CCGCTC 1 cut(s) 3941
MfeI CAATTG 6 cut(s) 71, 1404, 2693, 3289, 5780, 6192
MflI RGATCY 6 cut(s) 826, 2191, 2863, 4438, 4781, 5555
MhlI GDGCHC 5 cut(s) 1251, 3061, 3783, 4249, 5259
MlsI TGGCCA 3 cut(s) 2077, 3551, 6040
MluI ACGCGT 1 cut(s) 2053
MluNI TGGCCA 3 cut(s) 2077, 3551, 6040
MlyI GAGTC 7 cut(s) 331, 793, 1293, 1466, 1610, 1723, 3711
MmeI TCCRAC 5 cut(s) 443, 685, 2831, 4645, 4781
Mox20I TGGCCA 3 cut(s) 2077, 3551, 6040
Mph1103I ATGCAT 1 cut(s) 1380
MroI TCCGGA 2 cut(s) 3365, 4441
MroXI GAANNNNTTC 5 cut(s) 1218, 2559, 4975, 5028, 5702
MscI TGGCCA 3 cut(s) 2077, 3551, 6040
MslI CAYNNNNRTG 8 cut(s) 394, 561, 1594, 1678, 2274, 2762, 4545, 5191
Msp20I TGGCCA 3 cut(s) 2077, 3551, 6040
MspA1I CMGCKG 2 cut(s) 167, 2471
MspCI CTTAAG 1 cut(s) 3721
MspI CCGG 6 cut(s) 145, 152, 3366, 3745, 3964, 4442
MspR9I CCNGG 8 cut(s) 219, 1550, 1726, 3745, 4509, 4789, 5351, 5807
MunI CAATTG 6 cut(s) 71, 1404, 2693, 3289, 5780, 6192
Mva1269I GAATGC 4 cut(s) 1525, 1873, 3898, 4540
MvaI CCWGG 7 cut(s) 219, 1550, 1726, 4509, 4789, 5351, 5807
MvnI CGCG 4 cut(s) 537, 2055, 4291, 5172
NciI CCSGG 1 cut(s) 3745
NcoI CCATGG 1 cut(s) 2034
NdeI CATATG 3 cut(s) 1675, 2089, 2275
NheI GCTAGC 2 cut(s) 2198, 3175
NmuCI GTSAC 9 cut(s) 695, 998, 1265, 3240, 3410, 4292, 4522, 5318, 5570
NruI TCGCGA 1 cut(s) 5172
NsiI ATGCAT 1 cut(s) 1380
NspI RCATGY 7 cut(s) 271, 1566, 4169, 4349, 4565, 5070, 5755
NspV TTCGAA 1 cut(s) 1010
PaeI GCATGC 4 cut(s) 1566, 4169, 4565, 5755
PaeR7I CTCGAG 1 cut(s) 2857
PagI TCATGA 2 cut(s) 469, 3661
PciI ACATGT 2 cut(s) 267, 5066
PciSI GCTCTTC 1 cut(s) 5431
PctI GAATGC 4 cut(s) 1525, 1873, 3898, 4540
PdmI GAANNNNTTC 5 cut(s) 1218, 2559, 4975, 5028, 5702
PflMI CCANNNNNTGG 1 cut(s) 4889
PfoI TCCNGGA 1 cut(s) 1724
PleI GAGTC 7 cut(s) 330, 792, 1293, 1466, 1609, 1723, 3711
PmaCI CACGTG 2 cut(s) 1265, 4001
PmlI CACGTG 2 cut(s) 1265, 4001
PpsI GAGTC 7 cut(s) 330, 792, 1293, 1466, 1609, 1723, 3711
Ppu21I YACGTR 4 cut(s) 1258, 1265, 3415, 4001
PpuMI RGGWCCY 3 cut(s) 3740, 5809, 6175
PscI ACATGT 2 cut(s) 267, 5066
PshBI ATTAAT 1 cut(s) 4599
PsiI TTATAA 1 cut(s) 3947
Psp124BI GAGCTC 1 cut(s) 3061
Psp5II RGGWCCY 3 cut(s) 3740, 5809, 6175
Psp6I CCWGG 7 cut(s) 217, 1548, 1724, 4507, 4787, 5349, 5805
PspCI CACGTG 2 cut(s) 1265, 4001
PspEI GGTNACC 1 cut(s) 1168
PspFI CCCAGC 5 cut(s) 910, 964, 1145, 1705, 3185
PspGI CCWGG 7 cut(s) 217, 1548, 1724, 4507, 4787, 5349, 5805
PspPI GGNCC 7 cut(s) 611, 3740, 3848, 3961, 4571, 5809, 6175
PspPPI RGGWCCY 3 cut(s) 3740, 5809, 6175
PspXI VCTCGAGB 1 cut(s) 2857
PsrI GAACNNNNNNTAC 2 cut(s) 600, 632
PstI CTGCAG 5 cut(s) 1999, 2878, 4645, 5770, 6015
PstNI CAGNNNCTG 1 cut(s) 4784
PsuI RGATCY 6 cut(s) 826, 2191, 2863, 4438, 4781, 5555
PvuII CAGCTG 1 cut(s) 2471
RruI TCGCGA 1 cut(s) 5172
RseI CAYNNNNRTG 8 cut(s) 394, 561, 1594, 1678, 2274, 2762, 4545, 5191
SacI GAGCTC 1 cut(s) 3061
SalI GTCGAC 1 cut(s) 4476
SapI GCTCTTC 1 cut(s) 5431
Sau96I GGNCC 7 cut(s) 611, 3740, 3848, 3961, 4571, 5809, 6175
SbfI CCTGCAGG 1 cut(s) 1999
ScaI AGTACT 2 cut(s) 1756, 4829
SchI GAGTC 7 cut(s) 331, 793, 1293, 1466, 1610, 1723, 3711
ScrFI CCNGG 8 cut(s) 219, 1550, 1726, 3745, 4509, 4789, 5351, 5807
SdaI CCTGCAGG 1 cut(s) 1999
SduI GDGCHC 5 cut(s) 1251, 3061, 3783, 4249, 5259
SfcI CTRYAG 7 cut(s) 1242, 1995, 2874, 4641, 5277, 5766, 6011
Sfr274I CTCGAG 1 cut(s) 2857
SfuI TTCGAA 1 cut(s) 1010
SinI GGWCC 5 cut(s) 3740, 3848, 4571, 5809, 6175
SlaI CTCGAG 1 cut(s) 2857
SmiMI CAYNNNNRTG 8 cut(s) 394, 561, 1594, 1678, 2274, 2762, 4545, 5191
SmlI CTYRAG 5 cut(s) 1684, 2534, 2857, 3721, 3956
SmoI CTYRAG 5 cut(s) 1684, 2534, 2857, 3721, 3956
SnaBI TACGTA 1 cut(s) 1258
SpeI ACTAGT 1 cut(s) 5599
SphI GCATGC 4 cut(s) 1566, 4169, 4565, 5755
Sse8387I CCTGCAGG 1 cut(s) 1999
SsiI CCGC 9 cut(s) 58, 165, 360, 676, 2353, 2370, 3939, 4500, 5476
SspI AATATT 2 cut(s) 2254, 3613
SstI GAGCTC 1 cut(s) 3061
StyD4I CCNGG 8 cut(s) 217, 1548, 1724, 3743, 4507, 4787, 5349, 5805
StyI CCWWGG 7 cut(s) 2034, 3764, 3926, 4527, 6035, 6171, 6178
TaiI ACGT 7 cut(s) 1178, 1260, 1267, 3417, 4003, 5055, 5686
TaqII GACCGA 1 cut(s) 4567
TatI WGTACW 8 cut(s) 774, 1042, 1754, 2766, 2838, 4062, 4827, 6102
TauI GCSGC 2 cut(s) 2372, 3941
TseFI GTSAC 9 cut(s) 695, 998, 1265, 3240, 3410, 4292, 4522, 5318, 5570
Tsp45I GTSAC 9 cut(s) 695, 998, 1265, 3240, 3410, 4292, 4522, 5318, 5570
TspGWI ACGGA 4 cut(s) 248, 429, 2996, 4311
Van91I CCANNNNNTGG 1 cut(s) 4889
Vha464I CTTAAG 1 cut(s) 3721
VneI GTGCAC 1 cut(s) 5255
VpaK11BI GGWCC 5 cut(s) 3740, 3848, 4571, 5809, 6175
VspI ATTAAT 1 cut(s) 4599
XagI CCTNNNNNAGG 1 cut(s) 2958
XbaI TCTAGA 3 cut(s) 760, 3713, 4208
XceI RCATGY 7 cut(s) 271, 1566, 4169, 4349, 4565, 5070, 5755
XcmI CCANNNNNNNNNTGG 2 cut(s) 3691, 4809
XhoI CTCGAG 1 cut(s) 2857
XmaJI CCTAGG 1 cut(s) 3926
XmiI GTMKAC 2 cut(s) 1837, 4477
XmnI GAANNNNTTC 5 cut(s) 1218, 2559, 4975, 5028, 5702
ZrmI AGTACT 2 cut(s) 1756, 4829
Zsp2I ATGCAT 1 cut(s) 1380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.