MD04G1129300.v1.1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0007182
Physical Location & Seq
Reverse (-)
21508783 .. 21521490
12708 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1129300.v1.1.491

Sequence Viewer

Length: 6093 bp
ATGGAAGCCGATTTGCCTCCTGAATACGCCCACATCAAGCATCGTGATCGAATCGTGCGGAGGCTAGCTCAACTGGGAGTGCCAAACGAGTTTCTTGGTGAATACCAAAGTGGGTTAGTTGCTTTTATTAAGAATAACAAGCAACGGATACCAGAACTGGTTTCCGCTATACTACCTGCTGATGACGAAGTGGCTCTAAGTGAAGCAAAACCAGGGACTAAGAAACAAGGTGTAAGCATGAAAAGTCGATTCCACGAAAGCATGTTGTGGTTGCAGTGGTTAATGTTTGAGGGTGAACCTATGTCAGCCCTGAAGAGTCTCTCCAAAATGAGTGTTGGGCAGCGTGGTATTTGTGGAGCTGTTTGGGGACATAACGATATAGCTTACAGATGCCGAACATGTGCAAATGATCCAACATGTGCAATTTGTTTTCCTTGCTTTCAGAATGGGAACCACAAGGATCATGACTATTCAATTATTTATACTGGTGGCGGTTGTTGTGATTGTGGTGATGTTACAGCATGGAAGCGTGAAGGCTTTTGCTCAAATCATAAAGGTGCGGAACAGATACAACCTCTTCCAAAGGAGATTGCAAACATTGTAGGGCCTGTTCTTGATTGCCTCTTTGATATATGGAAAACCAAGCTGGTTCTTGGTGAAACTATCTGGCGGGAAAATGCTAGAGCAAGTGACCAAGTTGGAGAACGTAGAAAGGTTGAAAATGAGTTAACTTTTTTCGTGGTTGAGATGCTTCTAGAATTCTGTAAGTACAGTGAGAGTTTGCTAAGTTTTGTATCCAAGACGGTTTTAGCATCAGGGGATCTATTGGGGATTCTTGTGAGGGCAGAAAGGTTTTTGAATGACACTGTTACAAAAAGGCTGCATGAGTTGCTACTGAAACTGCTGGGAGAACCTGTTTTCAAATATGAGTTTGCCAAAGTATTTTTGAGTTATTACCCAGCTGTTGTAAGTGAAGCGGAAGCCAGAAAGGAATTAAGCGACCTTTCTGTGAAAAAGTATCCACTGCTCTTTGCATTCTCTGTTCAAATCTTCACGGTGCCAACTTTAACACCTCGTCTTGTGAAAGAAATGAACCTGCTGTCTATGCTGATGGGATGCTTGGAAGATATTTTCAGTTCCTGTGCTGGGGATGACGGTCGTTTACAGGTTACCAATTTGTCAAATTTGTATGAGATCACGATTCGTGTGATTGAAGATATTAGATTTGTTATGAGCCATGCTACAGTGCCCAAATATGTAACTCATGACCAGCAAAATATTTCAAGAACTTGGATGAGACTCTTGGCGTTCGTGCAAGGCATGAACCCTCAAAAAAGGGAAACAGGCCTGCACATTGAAGAAGAAAATGAGAATATGCATCTGCTGTTTGTTCTAGGTCATTCAATTACCAACATTCATTCTTTATTGGTGGATGGGGCATTTTCTGTAGCTATTGACAAGATGGATGAAGACTCTCTTTTCAGAACATATAAGCAGGATACGGATGACAGGGATAGCCTAAGACATTCAAAAGTAGGACGGCTGTCTCAGGAAAGTTCTGCATGTAGTGCAGTGGGGAGTAGTTCATTTGCGTGTGAGTCGAAGGTTCCTGAAGATAAATCAGATGCTCTTTCTGATTTGTTGATTCCACCATCTGTGATGTGGCTAACATATGAGTGCTTGAGGGCAATTGAGAATTGGCTGGGAGGTGATAATACCTCCAGGACTCTTCTTGATGCATCTTCTCCAAGTACTAGCAATTTATCTCCTAGCAATTTTTCAGCATTGAAGAGAACATTATCCAAGATCAGGAAAGGAAAATATATTTTTGGTAGACTTGCCAGTTCTAGTGAAGATCATGGCAAGCAGTGTTCATCACATTTACATAGTGATTGCAATATGAGTGTTGACACCCAAAAAGGCAAAATTGCAGGGCAGGAGAGTAATTTGATGGTTACTGACAACATTGATTCAGTTAATGCCTGCAATCCTGCAGGATTTGATGATAGTGCTATGGAAGTAGATGGTGCCATGGACTTAGATGCTCTACGTGTATTGAGTTTGTCTGATTGGCCTGACATAACATATGATGTTAGTTCACAGGATATATCTGTTCACATTCCTTTACATCGCTTACTTTCCTTGCTTTTGCAGAAAGCATTAAGAAGATGTTTTGGTGAAGTGCCAGATCTGGCTAGCGTTACTTCTGCAAATTCATCATCAGCTATTTTAACTGACTTCTTTGGAAATATTCTGGGAGGCTGCCACCCATATGGATTTTCTGCCTTTGTTATGGAGCATCCCCTTCGGATCAGAGTATTTTGTGCGGAGGTTCATGCGGGAATGTGGCGGAAAAATGGGGATGCCGCCCTATTATCTTGTGAGTGGTATCGTTCAGTTCGCTGGTCTGAACAGGGTTTGGAGCTTGATCTGTTTCTGCTTCAATGCTGTGCTGCCTTGGCTCCAGCTGATCCTTATGTTAACAGAATTATTGAACGCTTTGGCCTGTCCAGTTACCTTTCTTTGAACCTTGAGCACTCTAGTGAATATGAGACTGTTCTAGTGCAAGAAATGCTCACTCTTATTATACAAATAGTCAAAGAAAGGCGATTTTGTGGTCTAACTAAAGCTGAAAGTTTGAAAAGAGAGTTGGTTCATAAATTAGCCATTGCAGATGGCACTCATAGTCAATTGGTTAAATCTCTTCCTCGTGATCTGTCCAAGTCTGATCAGCTACATGGAATTTTGGAATCCATTGCCGTGTACTCCAATCCCTCTGGCTTTAATCAGGGGACTTATTCACTACAATGGACATTTTGGAAAGAACTGGATTTGTACTACCCTCGTTGGAACTCGAGGGATCTGCAAGCTGCTGAAGAAAGATACTTGCGTTTTCGTAGTGTTTCTGCATTGACCACTCAGCTACCCAGATGGACTAAGATTTATTCTCCTTTCAAAGGGGTAGCTAGAATAGCTACATGTAAAGCAGTCCTTAAAATTATCCGTGCTGTGCTGTTTTATGCTGTTTCTTCTGATAAATCAAGTGATTCACGAGCTCCTGATAGTGTTCTGTTAAATGCGTTGCACTTACTTTCGTTAGCATTAGACATTTGTTCTCAACATAAGGAAGCTGGTGATAAGTCTTGTTATGATGGAGATGCAATTCCCATGCTAGCTTTTGCTGGGGAAGAAATTAATGAAGGACGATACTTTGGTGCTGGTCAACAAAGCTTGCTGTCACTTCTTGTTATCTTGATGAGGATGTATAAGAAACAGAATGTCAACAATTGTTTGGAAGCTGGAAGTTGCGACCTGTCTTCTTTAATTGGAAGTTTATTAAAGAAGTTTGTAGAGATTGATTCCGGATGTTTGACCATATTACAACAACTTGCACCTGAAGTGGTTGGTCTTGTGTCGCAATCTTCTCCAAATGTTGACGCTAAGACCTCAGGTTCAATTTCTGACTCTGAGAAGCGCAAGGCAAAAGCCAGAGAGAGACAGGCTGCCATATTGGAAAAAATGAGAGCCGAACAATCCAAGTTTATGGCCAGTGTTAAATCCACTGTGGACAAGGGTTCAAAATCGGAGCAAGAGGTTTGTGAACTTAATGTTGAAGATGATTTGGAAGAGTCTGAACAAGTTGTTTGCTCTCTTTGTCATGATCCCAGTTCCAGAAATCCAATATCCTACTTGGTTTTACTTCAGAAATCTAGACTTTTGAGTTTCATAGAAAGAGGTCCCCTGTCATGGGAGCAACCACGTTGGATAGACAAGGAGCACATCTCTATTACCACAAACGAGGTGAATGATCAATCCGGATTAAGCACTTTATCAAGTGGTTCAGGACCACAACTGGTGCCATCTGATCCTTGTAGACATTTGGTTCAGAATGCAGTCACCGAATTTGCCTGTCATGGACAACCTAGGGATGTTGACGCTCTTATAGATTTTCTCAAGGGCCGGTTCCATGAGTTAAGGAATATCCAAGTGCAACGTGAATCAAATGATGAAAGGGAAAAGACTTTACATACATTTGAGACAATGGAAGATGATATGTACATCTGCATCCAAAAGGAAATGCATGATAAGATGCTTCATTCTAATCCTTTAGAGGATAAAGGATTTTCAACTCCTGAAGGGGATCAAGAAGAAACTAAGCATGCTGAATTTATGTTACTTGGAAAATATATAGCTTCTCTTTCTAGAGAGACAACAGAGAATCCTTCATCTTCCGAAAGTGCTCCTAATGAAAGGGTGACAGTAGATTCTTCTCGTCTTTCAGCACATGACGGATTTGGCCCAACAGATTGTGATGGAATTTATCTTTCTTCCTGTGGACATGCTGTACATCAGGGATGCCTTGATCGTTATTTATCTTCGTTGAAGGAAAGGTATCTCAGAAGAATTGTTTTTGAAGGGGGACATATTGCAGATCCCGACAAGGGGGAGTTTCTCTGTCCTGTATGCCGTCGACTTGCAAATTCTGTCTTGCCCGCATTGCCAGAGTTTCTCCAAGTCACTAAGGAGCCTCCGTATTCAGGTGTTAGTTCATCGCATGCTACTGGTCAATTGGTCAAATCAGGTGACGAAATTAATTCTCTTCAGCTTCAACAAGGGTTGGCTCTCTTGCAATCTGCAGCAGAGGCATCTGGGAAGGTTGGAAGTCTTAAAGGTTTCCCCCTTCGGAGATGTGGAAGAATGACCTCAGACCTTGAACCAATTTTTCAATCGCTCTGTAAGATGTATTTTCTGACTAAACAGGATAAGTTATCAGGATCTGCCAGGGTCAACCACCCAATGCTTATGTGGGACACAATTAAGTACTCTCTACTATCAACAGAAATTGCTGCTCGTTCTGACGTTGGCGATGAAACATGTGGACAAGGTGCCATGCTGCGTATCTTGGAACATGTTGATCTAGCGGTATCATATCCTGATATTCAGTTCTGGAATCGAGCTTCTGATCCTGTCCTTGCTCGTGATCCTTTTTCATCATTGATGTGGGTTCTTTTTTGTCTTCCAAACCAATTTTTATCATGTGAAGATTCATTGTTATCCCTAGTGCACTTATTTTATGTTGTCTCTGTAGTTCAGGGCATAATGACATATTGTGGGGAAAATCAATGTGACATTAGTGGATTAGGTATTGATGATTGCCTGGTTACTGACATCTCTAAACTTATGGAAGAATCTGGAGCCACCCAGCAATATTTCGTTTCAAACTATATTGGCTCTTCTTGTAATATTAAGAACATAGTTTGCAGCCTGAGTTTCCCGTATTTGCGGAGGTGCGCATTGCTGTTGAAGCTGCTGAACTCTTGTTCCCGAGTACCATTCCATGAAAGGTTTAATGTCTTGGATAGATCTCATGCCATTGGTGACATGATGGACACCACTTATGGGGCACTAGTGAATCTAAATGATGTTCAAGAAATTGAAAGCATGTTCAAGGTTCCTACACTGGATGTTATCTTTAAGGATAAAGTGGTACGTTCAATAGCTCAGAAATGGTTCCATCATTTTCGGAAGGAGTTGGAGGTCCTAAGATTTCAAGGAAGCATGCACTGTAGCCCTGCAGTTCCCTTCCAATTGATGCGTCTGCCCCGAGTTTACCAAGACTTATTGCAGAGATATATAAAACAGCGTTGTCCTGATTGCGAGATTATTCTTGATGACCCTGCATTGTGCTTGCTTTGTGGGAGATTATGCTCTCCAAGCTGGAAGTCATGTTGCAAGGAAAGTGGATGCCAAACTCATGCAGTGTCCTGTGGTTCTGGTACTGGGGTATTTCTGTTGATCAGGAGGACAACAATTCTGCTGCAAAGATCTGCACGGCAAGCTCCCTGGCCATCACCCTACTTGGATGCTTTTGGAGAAGAGGATATTGAAATGCAGAGAGGAAAGCCGCTGTACTTGAACGAGGAACGTTATGCAGCTTTAACTTACTTGGTTGCTTCTCATGGCCTTGATCGAAGCTCCAAGGTTCTTGGACAAACTACTATTGGTTCTTTCTTCATGGTGGCATATATGTTGACACAGATTGATGGCAAAGGATTCTTGATAGCAGCAGATAATATCGACTTCCGACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000151 GO:0000280 GO:0001101 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006259 GO:0006310 GO:0006325 GO:0006342 GO:0006355 GO:0006464 GO:0006508 GO:0006511 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0007049 GO:0007127 GO:0007131 GO:0007140 GO:0007141 GO:0007276 GO:0007283 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009605 GO:0009607 GO:0009620 GO:0009719 GO:0009725 GO:0009737 GO:0009889 GO:0009890 GO:0009892 GO:0009894 GO:0009966 GO:0009968 GO:0009987 GO:0010029 GO:0010033 GO:0010243 GO:0010468 GO:0010498 GO:0010528 GO:0010529 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010646 GO:0010648 GO:0016043 GO:0016458 GO:0016567 GO:0016569 GO:0016570 GO:0016574 GO:0016597 GO:0016740 GO:0019216 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0019941 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0023051 GO:0023057 GO:0030163 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031406 GO:0032006 GO:0032007 GO:0032446 GO:0032501 GO:0032504 GO:0032991 GO:0033522 GO:0033993 GO:0034641 GO:0035825 GO:0036094 GO:0036211 GO:0040029 GO:0042221 GO:0043161 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043177 GO:0043200 GO:0043201 GO:0043207 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0044703 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048232 GO:0048285 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0048609 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0050994 GO:0051171 GO:0051172 GO:0051239 GO:0051252 GO:0051253 GO:0051276 GO:0051321 GO:0051603 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0061630 GO:0061659 GO:0061982 GO:0065007 GO:0070647 GO:0070728 GO:0070887 GO:0071229 GO:0071230 GO:0071233 GO:0071310 GO:0071417 GO:0071495 GO:0071596 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097305 GO:0098542 GO:0140013 GO:0140096 GO:1900140 GO:1901360 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902494 GO:1902531 GO:1902532 GO:1902679 GO:1903046 GO:1903506 GO:1903507 GO:1990234 GO:2000026 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-UBR

Protein Analysis

2031

Amino Acids

227.12

Weight (kDa)

5.81

Isoelectric Point (pI)

48.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-UBR PF02207 118 - 184 7.8e-20 Putative zinc finger in N-recognin (UBR box)
WHD_UBR1 PF22960 880 - 966 1.5e-19 E3 ubiquitin-protein ligase UBR1-like, winged-helix domain
PRT6_C PF18995 1642 - 1985 6.7e-81 Proteolysis_6 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 5293
Acc36I ACCTGC 2 cut(s) 184, 1104
AccB1I GGYRCC 4 cut(s) 1057, 2027, 3856, 4886
AccI GTMKAC 3 cut(s) 1834, 3874, 4471
AccIII TCCGGA 2 cut(s) 3362, 3815
AclI AACGTT 1 cut(s) 5926
AcoI YGGCCR 2 cut(s) 3546, 5846
AcsI RAATTY 8 cut(s) 758, 1183, 2212, 2742, 3902, 4166, 4317, 4480
AcuI CTGAAG 7 cut(s) 332, 1632, 2895, 3417, 3686, 4155, 4586
AfiI CCNNNNNNNGG 9 cut(s) 1146, 1809, 2957, 3747, 3748, 4442, 4443, 4538, 5400
AflIII ACRYGT 6 cut(s) 398, 416, 2050, 2978, 4874, 4909
AhlI ACTAGT 1 cut(s) 5407
AjnI CCWGG 5 cut(s) 211, 1721, 4781, 5157, 5843
AjuI GAANNNNNNNTTGG 2 cut(s) 2633, 2665
AleI CACNNNNGTG 1 cut(s) 2541
AloI GAACNNNNNNTCC 2 cut(s) 5306, 5338
Alw21I GWGCWC 5 cut(s) 2538, 3058, 3780, 4243, 5067
Alw26I GTCTC 8 cut(s) 323, 1291, 1551, 2546, 3490, 4031, 4202, 5086
Alw44I GTGCAC 1 cut(s) 5063
AlwNI CAGNNNCTG 2 cut(s) 1140, 4778
Ama87I CYCGRG 3 cut(s) 2854, 5325, 5604
Aor13HI TCCGGA 2 cut(s) 3362, 3815
AoxI GGCC 9 cut(s) 605, 1345, 2072, 2503, 3546, 3958, 4297, 5846, 5962
ApaLI GTGCAC 1 cut(s) 5063
ApoI RAATTY 8 cut(s) 758, 1183, 2212, 2742, 3902, 4166, 4317, 4480
AseI ATTAAT 2 cut(s) 3195, 4593
Asp700I GAANNNNTTC 1 cut(s) 5510
AspA2I CCTAGG 1 cut(s) 3923
AspLEI GCGC 2 cut(s) 3477, 5294
AspS9I GGNCC 6 cut(s) 605, 3737, 3845, 3958, 4298, 5539
AsuNHI GCTAGC 3 cut(s) 64, 2195, 3172
AvaI CYCGRG 3 cut(s) 2854, 5325, 5604
AvaII GGWCC 3 cut(s) 3737, 3845, 5539
AvrII CCTAGG 1 cut(s) 3923
AxyI CCTNAGG 1 cut(s) 3448
BaeGI GKGCMC 3 cut(s) 1251, 5067, 5407
BaeI ACNNNNGTAYC 6 cut(s) 2875, 2908, 3199, 3232, 5769, 5802
BalI TGGCCA 2 cut(s) 3548, 5848
BanI GGYRCC 4 cut(s) 1057, 2027, 3856, 4886
BanII GRGCYC 1 cut(s) 3058
BauI CACGAG 3 cut(s) 2709, 3051, 4977
BbsI GAAGAC 3 cut(s) 1476, 3309, 5009
Bbv12I GWGCWC 5 cut(s) 2538, 3058, 3780, 4243, 5067
BceAI ACGGC 4 cut(s) 1556, 2744, 4452, 5849
BcgI CGANNNNNNTGC 6 cut(s) 29, 63, 2288, 2322, 2845, 2879
BciT130I CCWGG 5 cut(s) 213, 1723, 4783, 5159, 5845
BciVI GTATCC 4 cut(s) 141, 805, 1029, 1492
BclI TGATCA 3 cut(s) 2728, 3808, 5796
BcoDI GTCTC 8 cut(s) 323, 1291, 1551, 2546, 3490, 4031, 4202, 5086
BcuI ACTAGT 1 cut(s) 5407
BfmI CTRYAG 7 cut(s) 1242, 1446, 1992, 4635, 5085, 5566, 5574
BfuAI ACCTGC 2 cut(s) 184, 1104
BfuI GTATCC 4 cut(s) 141, 805, 1029, 1492
BglII AGATCT 3 cut(s) 2188, 5363, 5825
BlnI CCTAGG 1 cut(s) 3923
BmcAI AGTACT 2 cut(s) 1753, 4823
Bme1390I CCNGG 5 cut(s) 213, 1723, 4783, 5159, 5845
Bme18I GGWCC 3 cut(s) 3737, 3845, 5539
BmeT110I CYCGRG 3 cut(s) 2854, 5325, 5604
BmgT120I GGNCC 6 cut(s) 605, 3737, 3845, 3958, 4298, 5539
BmrFI CCNGG 5 cut(s) 213, 1723, 4783, 5159, 5845
BmrI ACTGGG 3 cut(s) 83, 3660, 5790
BmtI GCTAGC 3 cut(s) 68, 2199, 3176
BmuI ACTGGG 3 cut(s) 83, 3660, 5790
BoxI GACNNNNGTC 1 cut(s) 1543
BpiI GAAGAC 3 cut(s) 1476, 3309, 5009
BplI GAGNNNNNCTC 4 cut(s) 52, 84, 3767, 3799
BpmI CTGGAG 3 cut(s) 1705, 2448, 5214
BpuEI CTTGAG 3 cut(s) 1702, 2552, 3938
BsaAI YACGTR 1 cut(s) 2051
BsaJI CCNNGG 7 cut(s) 212, 2031, 2457, 3923, 4782, 5843, 5979
BsaWI WCCGGW 2 cut(s) 3362, 3815
BsaXI ACNNNNNCTCC 4 cut(s) 900, 930, 4517, 4547
Bsc4I CCNNNNNNNGG 9 cut(s) 1146, 1809, 2957, 3747, 3748, 4442, 4443, 4538, 5400
Bse118I RCCGGY 1 cut(s) 3960
Bse21I CCTNAGG 1 cut(s) 3448
Bse3DI GCAATG 4 cut(s) 2667, 2754, 4496, 5294
BseAI TCCGGA 2 cut(s) 3362, 3815
BseBI CCWGG 5 cut(s) 213, 1723, 4783, 5159, 5845
BseDI CCNNGG 7 cut(s) 212, 2031, 2457, 3923, 4782, 5843, 5979
BseLI CCNNNNNNNGG 9 cut(s) 1146, 1809, 2957, 3747, 3748, 4442, 4443, 4538, 5400
BseMI GCAATG 4 cut(s) 2667, 2754, 4496, 5294
BseMII CTCAG 8 cut(s) 1562, 2933, 3459, 3462, 4411, 4719, 5258, 5516
BseSI GKGCMC 3 cut(s) 1251, 5067, 5407
BseYI CCCAGC 6 cut(s) 904, 958, 1145, 1702, 3182, 5202
BsgI GTGCAG 3 cut(s) 1334, 1590, 5814
Bsh1285I CGRYCG 1 cut(s) 1159
BshFI GGCC 9 cut(s) 607, 1347, 2074, 2505, 3548, 3960, 4299, 5848, 5964
BshNI GGYRCC 4 cut(s) 1057, 2027, 3856, 4886
BsiEI CGRYCG 1 cut(s) 1159
BsiHKAI GWGCWC 5 cut(s) 2538, 3058, 3780, 4243, 5067
BsiHKCI CYCGRG 3 cut(s) 2854, 5325, 5604
BsiSI CCGG 3 cut(s) 3363, 3816, 3961
BslFI GGGAC 6 cut(s) 229, 381, 2806, 3723, 4434, 4823
BslI CCNNNNNNNGG 9 cut(s) 1146, 1809, 2957, 3747, 3748, 4442, 4443, 4538, 5400
BsmAI GTCTC 8 cut(s) 323, 1291, 1551, 2546, 3490, 4031, 4202, 5086
BsmFI GGGAC 6 cut(s) 229, 381, 2806, 3723, 4434, 4823
BsmI GAATGC 2 cut(s) 1034, 3895
BsnI GGCC 9 cut(s) 607, 1347, 2074, 2505, 3548, 3960, 4299, 5848, 5964
BsoBI CYCGRG 3 cut(s) 2854, 5325, 5604
Bsp1286I GDGCHC 7 cut(s) 1251, 2538, 3058, 3780, 4243, 5067, 5407
Bsp13I TCCGGA 2 cut(s) 3362, 3815
Bsp1407I TGTACA 2 cut(s) 4056, 4345
Bsp19I CCATGG 1 cut(s) 2031
BspANI GGCC 9 cut(s) 607, 1347, 2074, 2505, 3548, 3960, 4299, 5848, 5964
BspCNI CTCAG 8 cut(s) 1561, 2932, 3460, 3461, 4410, 4718, 5259, 5515
BspEI TCCGGA 2 cut(s) 3362, 3815
BspHI TCATGA 3 cut(s) 463, 1264, 3658
BspMAI CTGCAG 3 cut(s) 1996, 4639, 5578
BspMI ACCTGC 2 cut(s) 184, 1104
BspOI GCTAGC 3 cut(s) 68, 2199, 3176
BspQI GCTCTTC 1 cut(s) 5239
BspT107I GGYRCC 4 cut(s) 1057, 2027, 3856, 4886
BsrDI GCAATG 4 cut(s) 2667, 2754, 4496, 5294
BsrFI RCCGGY 1 cut(s) 3960
BsrGI TGTACA 2 cut(s) 4056, 4345
BssAI RCCGGY 1 cut(s) 3960
BssECI CCNNGG 7 cut(s) 212, 2031, 2457, 3923, 4782, 5843, 5979
BssSI CACGAG 3 cut(s) 2709, 3051, 4977
BssT1I CCWWGG 4 cut(s) 2031, 2457, 3923, 5979
Bst2BI CACGAG 3 cut(s) 2709, 3051, 4977
Bst2UI CCWGG 5 cut(s) 213, 1723, 4783, 5159, 5845
Bst6I CTCTTC 9 cut(s) 308, 582, 1734, 1784, 2709, 3621, 4605, 5239, 5871
BstAPI GCANNNNNTGC 3 cut(s) 889, 1568, 2572
BstAUI TGTACA 2 cut(s) 4056, 4345
BstBAI YACGTR 1 cut(s) 2051
BstDSI CCRYGG 1 cut(s) 2031
BstEII GGTNACC 1 cut(s) 1168
BstENI CCTNNNNNAGG 1 cut(s) 2955
BstHHI GCGC 2 cut(s) 3477, 5294
BstMAI GTCTC 8 cut(s) 323, 1291, 1551, 2546, 3490, 4031, 4202, 5086
BstMCI CGRYCG 1 cut(s) 1159
BstNI CCWGG 5 cut(s) 213, 1723, 4783, 5159, 5845
BstPAI GACNNNNGTC 1 cut(s) 1543
BstPI GGTNACC 1 cut(s) 1168
BstSCI CCNGG 5 cut(s) 211, 1721, 4781, 5157, 5843
BstSFI CTRYAG 7 cut(s) 1242, 1446, 1992, 4635, 5085, 5566, 5574
BstSLI GKGCMC 3 cut(s) 1251, 5067, 5407
BstV2I GAAGAC 3 cut(s) 1476, 3309, 5009
BstX2I RGATCY 7 cut(s) 820, 2188, 2860, 4432, 4775, 5363, 5825
BstXI CCANNNNNNTGG 2 cut(s) 2273, 3544
BstYI RGATCY 7 cut(s) 820, 2188, 2860, 4432, 4775, 5363, 5825
Bsu36I CCTNAGG 1 cut(s) 3448
BsuI GTATCC 4 cut(s) 141, 805, 1029, 1492
BsuRI GGCC 9 cut(s) 607, 1347, 2074, 2505, 3548, 3960, 4299, 5848, 5964
BtgI CCRYGG 1 cut(s) 2031
BtgZI GCGATG 3 cut(s) 2114, 4536, 4881
BtsI GCAGTG 5 cut(s) 281, 1022, 1578, 1874, 5766
BveI ACCTGC 2 cut(s) 184, 1104
CaiI CAGNNNCTG 2 cut(s) 1140, 4778
CciI TCATGA 3 cut(s) 463, 1264, 3658
CfoI GCGC 2 cut(s) 3477, 5294
Cfr10I RCCGGY 1 cut(s) 3960
Cfr13I GGNCC 6 cut(s) 605, 3737, 3845, 3958, 4298, 5539
CseI GACGC 3 cut(s) 3446, 3944, 5585
CspCI CAANNNNNGTGG 2 cut(s) 5722, 5757
EaeI YGGCCR 2 cut(s) 3546, 5846
Eam1104I CTCTTC 9 cut(s) 308, 582, 1734, 1784, 2709, 3621, 4605, 5239, 5871
EarI CTCTTC 9 cut(s) 308, 582, 1734, 1784, 2709, 3621, 4605, 5239, 5871
EciI GGCGGA 1 cut(s) 2365
Ecl136II GAGCTC 1 cut(s) 3056
Eco130I CCWWGG 4 cut(s) 2031, 2457, 3923, 5979
Eco147I AGGCCT 1 cut(s) 1347
Eco24I GRGCYC 1 cut(s) 3058
Eco47I GGWCC 3 cut(s) 3737, 3845, 5539
Eco53kI GAGCTC 1 cut(s) 3056
Eco57I CTGAAG 7 cut(s) 332, 1632, 2895, 3417, 3686, 4155, 4586
Eco81I CCTNAGG 1 cut(s) 3448
Eco88I CYCGRG 3 cut(s) 2854, 5325, 5604
Eco91I GGTNACC 1 cut(s) 1168
EcoICRI GAGCTC 1 cut(s) 3056
EcoNI CCTNNNNNAGG 1 cut(s) 2955
EcoO109I RGGNCCY 3 cut(s) 605, 3737, 5539
EcoO65I GGTNACC 1 cut(s) 1168
EcoRI GAATTC 1 cut(s) 758
EcoRII CCWGG 5 cut(s) 211, 1721, 4781, 5157, 5843
EcoT14I CCWWGG 4 cut(s) 2031, 2457, 3923, 5979
EcoT22I ATGCAT 3 cut(s) 1380, 1741, 4083
EcoT38I GRGCYC 1 cut(s) 3058
ErhI CCWWGG 4 cut(s) 2031, 2457, 3923, 5979
FalI AAGNNNNNCTT 4 cut(s) 1461, 1493, 2976, 3008
FaqI GGGAC 6 cut(s) 229, 381, 2806, 3723, 4434, 4823
FauI CCCGC 3 cut(s) 663, 2332, 4501
FauNDI CATATG 3 cut(s) 1672, 2086, 2272
FbaI TGATCA 3 cut(s) 2728, 3808, 5796
FblI GTMKAC 3 cut(s) 1834, 3874, 4471
FriOI GRGCYC 1 cut(s) 3058
FspAI RTGCGCAY 1 cut(s) 5293
FspI TGCGCA 1 cut(s) 5293
GlaI GCGC 2 cut(s) 3476, 5293
GsaI CCCAGC 6 cut(s) 908, 962, 1149, 1706, 3186, 5206
GsuI CTGGAG 3 cut(s) 1705, 2448, 5214
HaeIII GGCC 9 cut(s) 607, 1347, 2074, 2505, 3548, 3960, 4299, 5848, 5964
HapII CCGG 3 cut(s) 3363, 3816, 3961
HgaI GACGC 3 cut(s) 3446, 3944, 5585
HhaI GCGC 2 cut(s) 3477, 5294
Hin6I GCGC 2 cut(s) 3475, 5292
HinP1I GCGC 2 cut(s) 3475, 5292
HindIII AAGCTT 1 cut(s) 3229
HpaI GTTAAC 2 cut(s) 729, 2482
HpaII CCGG 3 cut(s) 3363, 3816, 3961
Hpy99I CGWCG 1 cut(s) 4473
HpyCH4IV ACGT 7 cut(s) 706, 2050, 3760, 3994, 4860, 5491, 5926
HpySE526I ACGT 7 cut(s) 706, 2050, 3760, 3994, 4860, 5491, 5926
HspAI GCGC 2 cut(s) 3475, 5292
Kpn2I TCCGGA 2 cut(s) 3362, 3815
Ksp22I TGATCA 3 cut(s) 2728, 3808, 5796
KspAI GTTAAC 2 cut(s) 729, 2482
LguI GCTCTTC 1 cut(s) 5239
MaeII ACGT 7 cut(s) 706, 2050, 3760, 3994, 4860, 5491, 5926
MfeI CAATTG 5 cut(s) 1689, 2690, 3286, 4568, 5588
MflI RGATCY 7 cut(s) 820, 2188, 2860, 4432, 4775, 5363, 5825
MhlI GDGCHC 7 cut(s) 1251, 2538, 3058, 3780, 4243, 5067, 5407
MlsI TGGCCA 2 cut(s) 3548, 5848
MluNI TGGCCA 2 cut(s) 3548, 5848
MlyI GAGTC 7 cut(s) 325, 1293, 1466, 1607, 1720, 3458, 3638
MmeI TCCRAC 6 cut(s) 437, 679, 2828, 3743, 4639, 5514
Mox20I TGGCCA 2 cut(s) 3548, 5848
Mph1103I ATGCAT 3 cut(s) 1380, 1741, 4083
MroI TCCGGA 2 cut(s) 3362, 3815
MroXI GAANNNNTTC 1 cut(s) 5510
MscI TGGCCA 2 cut(s) 3548, 5848
MslI CAYNNNNRTG 8 cut(s) 555, 1591, 1675, 2271, 2541, 2759, 2806, 4999
Msp20I TGGCCA 2 cut(s) 3548, 5848
MspA1I CMGCKG 3 cut(s) 962, 2468, 5908
MspI CCGG 3 cut(s) 3363, 3816, 3961
MspR9I CCNGG 5 cut(s) 213, 1723, 4783, 5159, 5845
MunI CAATTG 5 cut(s) 1689, 2690, 3286, 4568, 5588
Mva1269I GAATGC 2 cut(s) 1034, 3895
MvaI CCWGG 5 cut(s) 213, 1723, 4783, 5159, 5845
NcoI CCATGG 1 cut(s) 2031
NdeI CATATG 3 cut(s) 1672, 2086, 2272
NheI GCTAGC 3 cut(s) 64, 2195, 3172
NmuCI GTSAC 8 cut(s) 689, 3237, 3895, 4255, 4516, 4583, 5126, 5378
NsbI TGCGCA 1 cut(s) 5293
NsiI ATGCAT 3 cut(s) 1380, 1741, 4083
OliI CACNNNNGTG 1 cut(s) 2541
PaeI GCATGC 3 cut(s) 4163, 4559, 5563
PaeR7I CTCGAG 1 cut(s) 2854
PagI TCATGA 3 cut(s) 463, 1264, 3658
PceI AGGCCT 1 cut(s) 1347
PciI ACATGT 5 cut(s) 398, 416, 2978, 4874, 4909
PciSI GCTCTTC 1 cut(s) 5239
PctI GAATGC 2 cut(s) 1034, 3895
PdmI GAANNNNTTC 1 cut(s) 5510
PfoI TCCNGGA 1 cut(s) 1721
PleI GAGTC 7 cut(s) 324, 1293, 1466, 1606, 1720, 3458, 3637
PpsI GAGTC 7 cut(s) 324, 1293, 1466, 1606, 1720, 3458, 3637
Ppu21I YACGTR 1 cut(s) 2051
PpuMI RGGWCCY 2 cut(s) 3737, 5539
PscI ACATGT 5 cut(s) 398, 416, 2978, 4874, 4909
PshAI GACNNNNGTC 1 cut(s) 1543
PshBI ATTAAT 2 cut(s) 3195, 4593
Psp124BI GAGCTC 1 cut(s) 3058
Psp1406I AACGTT 1 cut(s) 5926
Psp5II RGGWCCY 2 cut(s) 3737, 5539
Psp6I CCWGG 5 cut(s) 211, 1721, 4781, 5157, 5843
PspEI GGTNACC 1 cut(s) 1168
PspFI CCCAGC 6 cut(s) 904, 958, 1145, 1702, 3182, 5202
PspGI CCWGG 5 cut(s) 211, 1721, 4781, 5157, 5843
PspPI GGNCC 6 cut(s) 605, 3737, 3845, 3958, 4298, 5539
PspPPI RGGWCCY 2 cut(s) 3737, 5539
PspXI VCTCGAGB 1 cut(s) 2854
PsrI GAACNNNNNNTAC 2 cut(s) 594, 626
PstI CTGCAG 3 cut(s) 1996, 4639, 5578
PstNI CAGNNNCTG 2 cut(s) 1140, 4778
PsuI RGATCY 7 cut(s) 820, 2188, 2860, 4432, 4775, 5363, 5825
PvuII CAGCTG 2 cut(s) 962, 2468
RseI CAYNNNNRTG 8 cut(s) 555, 1591, 1675, 2271, 2541, 2759, 2806, 4999
SacI GAGCTC 1 cut(s) 3058
SalI GTCGAC 1 cut(s) 4470
SapI GCTCTTC 1 cut(s) 5239
Sau96I GGNCC 6 cut(s) 605, 3737, 3845, 3958, 4298, 5539
SbfI CCTGCAGG 1 cut(s) 1996
ScaI AGTACT 2 cut(s) 1753, 4823
SchI GAGTC 7 cut(s) 325, 1293, 1466, 1607, 1720, 3458, 3638
ScrFI CCNGG 5 cut(s) 213, 1723, 4783, 5159, 5845
SdaI CCTGCAGG 1 cut(s) 1996
SduI GDGCHC 7 cut(s) 1251, 2538, 3058, 3780, 4243, 5067, 5407
SfcI CTRYAG 7 cut(s) 1242, 1446, 1992, 4635, 5085, 5566, 5574
Sfr274I CTCGAG 1 cut(s) 2854
SinI GGWCC 3 cut(s) 3737, 3845, 5539
SlaI CTCGAG 1 cut(s) 2854
SmiMI CAYNNNNRTG 8 cut(s) 555, 1591, 1675, 2271, 2541, 2759, 2806, 4999
SmlI CTYRAG 4 cut(s) 1681, 2531, 2854, 3953
SmoI CTYRAG 4 cut(s) 1681, 2531, 2854, 3953
SpeI ACTAGT 1 cut(s) 5407
SphI GCATGC 3 cut(s) 4163, 4559, 5563
Sse8387I CCTGCAGG 1 cut(s) 1996
SseBI AGGCCT 1 cut(s) 1347
SspI AATATT 4 cut(s) 1279, 2251, 5210, 5245
SstI GAGCTC 1 cut(s) 3058
StuI AGGCCT 1 cut(s) 1347
StyD4I CCNGG 5 cut(s) 211, 1721, 4781, 5157, 5843
StyI CCWWGG 4 cut(s) 2031, 2457, 3923, 5979
TaiI ACGT 7 cut(s) 709, 2053, 3763, 3997, 4863, 5494, 5929
TaqI TCGA 8 cut(s) 49, 247, 1601, 2855, 4471, 4954, 5971, 6078
TatI WGTACW 8 cut(s) 768, 1751, 2763, 2835, 4056, 4345, 4821, 5910
TauI GCSGC 2 cut(s) 2369, 5908
TseFI GTSAC 8 cut(s) 689, 3237, 3895, 4255, 4516, 4583, 5126, 5378
Tsp45I GTSAC 8 cut(s) 689, 3237, 3895, 4255, 4516, 4583, 5126, 5378
TspGWI ACGGA 5 cut(s) 160, 1517, 2993, 4305, 4521
VneI GTGCAC 1 cut(s) 5063
VpaK11BI GGWCC 3 cut(s) 3737, 3845, 5539
VspI ATTAAT 2 cut(s) 3195, 4593
XagI CCTNNNNNAGG 1 cut(s) 2955
XapI RAATTY 8 cut(s) 758, 1183, 2212, 2742, 3902, 4166, 4317, 4480
XbaI TCTAGA 3 cut(s) 754, 3710, 4202
XcmI CCANNNNNNNNNTGG 2 cut(s) 1659, 4803
XhoI CTCGAG 1 cut(s) 2854
XmaJI CCTAGG 1 cut(s) 3923
XmiI GTMKAC 3 cut(s) 1834, 3874, 4471
XmnI GAANNNNTTC 1 cut(s) 5510
ZrmI AGTACT 2 cut(s) 1753, 4823
Zsp2I ATGCAT 3 cut(s) 1380, 1741, 4083
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.