Rroxscaffold_6G00414400

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0007182
Physical Location & Seq
Reverse (-)
36692762 .. 36702827
10066 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00414400.1

Sequence Viewer

Length: 2313 bp
ATGTCTACCATGGATATCGATTCCCCTTCTGAATCTCCCCCTCGTGATCGAATCGTTCAGAGGCTTGCTCTACTGGGAGTGCCTCAGAATCTTCTTCATCTGTATCATGTTGGTTTAGTTGCTTTTGTTAAAAATAACAAGCAGTTGTTACCGCGACTGGTTTCTGCTATCTTACCTCCTGATGAGGAAGTAGCGGAAGTTCTCCGCGAGGCCAAACCAGGGTCCAAGAAACAGTCAGCCGGTGTCACCATGAAACTTCAATTCCGCGAAAGCGTCTCGTGGCTAAAGTGGTTGATGTTTGAGAGTGACCCTGGCTTGGTTCTGAAGGGCCTGTCCAAAATGAGTGTCGGGCAGCGGGGCATTTGTGGAGCTGTTTGGGGACATAATGATATAGCCTTCAGGTGCAAAACATGCGAACATGATCCAACCTGTGCAATTTGCGTTCCGTGTTTTAAGAATGGGAACCACAAGGATCATGATTACTCTATTATCTATACCGGCGGTGGCTGTTGTGATTGCGGTGATGTTACAGCATGGAAGCGCCAAGGCTTTTGCTCAAAGCATAAAGGTGCAGAACAGATACAGCCCCTTCCAGAGGAGCTTGCAATCACCGTGGGGCCTGTTCTTCACTGCCTCTTTGCCTATTGGAAGAACAAGCTACTTCTTTCTGAAATTGCCTGCCGGGAAAACCCTAGGGTAACTGACCTTGCTGCAGAGAGTAAGAAGGTTGCAAATGAGCTAACCTATTTTGTGGTTGAGATGCTTCTGGACTTTTGCAAGTGCAGTGAGAGTTTGCTTAGCTTTGTATCGAAGATGATTTTATCATCAGTTGACTTTTTGGGGATTTTGGTGAGGGCAGAGAGGTTTTTGAGTGATGCTGTTGTGAAAAAGCTCCACGAGTTGCTCCTGAAATTGATGGGGGAGCCTGTTTTCAAATATGAGTTTGCCAAAGTATTTCTTAATTATTACCCAACTGTTGTTAGTGAAGCCATAAAGGAGTTCAAGGACCTTACTTTGAAAAAGTATCCGCTGCTCTCTATATTCTCTGTGCAAATCCTCACGGTGCCAACTTTAACACCCCGTCTTGTGAAAGAAATGAACCTACTGTCGATGCTGCTAGGATGTTTGGAAGATATTTTCAATTCCTGTGCTGGTGAAGACGGCCGTTTACAGGTTACCAAGTGGTCAAATTTGTATGACGTCACTATTCGTGTGATTGAAGATTTTCGGTTTGTCATGAGTCATGCTATTGTGCCAAAGTATGTAACTCATGAGCAGCAAGATATTGCGAGAACTTGGATGAGGCTCTCAGCTTTTGTGCAAGGGATGAATCCACAAAAGAGGGAAACAGGCCTCCATATTGAGGAGGAGAATGACAATATGCATCTGCCATTCGTTCTGGGTCATTCAATTGCCAACCTTCATTCTTTGTTGGTGGATGGAGCATTTTCTGTTGCTAGTGACAACACAGAAGAAGACTTATTTCTCGGCACAAATAAGCAAGATATGGATGACACAGATAGCCTAAGACATGCAAAAGTAGGACGGCTGTCCCAGGAAAGTTCTGCATGTAGTGCAGTGGGGAGTAGTTCATTTGCATGTGCATCGACGTTTTCTGAAGATAAATCAAATGCTCTTTTGAGGATACCATCATCTGTGATATGGTTAACATTTGAGTGTTTGAGGGCTATCGAGAATTGGCTGGGAGTTGATAACACCTCGTGTACAATTCATCATGTATCATCTTCAAGTACTAGCAATTTCTCTGGGAGCAATTTTTCAGCATTGAAGAGAACATTATCCAAAATTAGGAAAGGCAAAAACATTTTTGGTAGATTTGCCAGTTCAAGTGAAGACCATGGGAAGCATGGTTCATCACATTTACATAGTAGTTCTGATATGAGTGTTGACTTCCAGAATGGCAAAATTTCAGGGCAGGAAAATAAATTAATGGTTATGGACGAAATCGATCAAGCTAATGCCTGTAATTCGTCAGGATCCGGTGATAGTGCAATGGAAATAGATGGTCCCATGGACTTAGATGCTCTACGCGTGCTGAGTTCGTCCGACTGGCCAGACATAACATATGATGTTAGTTCGCAGGATATATCTGTTCACATTCCCTTACATCGCTTACTTGCGTTGCTTCTACAGAGATCATTAAGAAGGTGTTTTGGCGAAGTGCCAGATTCTATTAGAGCTAGTTCTGCAAATTTATCATCATCTATTTCCACGGACTTTTTGGAAATATTCTCGGAGGCTGCCACCCATGTGGGTTTTCTGCCTTTGTTATGGAGCATCCCCTTCGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000151 GO:0000280 GO:0001101 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006259 GO:0006310 GO:0006325 GO:0006342 GO:0006355 GO:0006464 GO:0006508 GO:0006511 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0007049 GO:0007127 GO:0007131 GO:0007140 GO:0007141 GO:0007276 GO:0007283 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009605 GO:0009607 GO:0009620 GO:0009719 GO:0009725 GO:0009737 GO:0009889 GO:0009890 GO:0009892 GO:0009894 GO:0009966 GO:0009968 GO:0009987 GO:0010029 GO:0010033 GO:0010243 GO:0010468 GO:0010498 GO:0010528 GO:0010529 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010646 GO:0010648 GO:0016043 GO:0016458 GO:0016567 GO:0016569 GO:0016570 GO:0016574 GO:0016597 GO:0016740 GO:0019216 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0019941 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0023051 GO:0023057 GO:0030163 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031406 GO:0032006 GO:0032007 GO:0032446 GO:0032501 GO:0032504 GO:0032991 GO:0033522 GO:0033993 GO:0034641 GO:0035825 GO:0036094 GO:0036211 GO:0040029 GO:0042221 GO:0043161 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043177 GO:0043200 GO:0043201 GO:0043207 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0044703 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048232 GO:0048285 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0048609 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0050994 GO:0051171 GO:0051172 GO:0051239 GO:0051252 GO:0051253 GO:0051276 GO:0051321 GO:0051603 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0061630 GO:0061659 GO:0061982 GO:0065007 GO:0070647 GO:0070728 GO:0070887 GO:0071229 GO:0071230 GO:0071233 GO:0071310 GO:0071417 GO:0071495 GO:0071596 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097305 GO:0098542 GO:0140013 GO:0140096 GO:1900140 GO:1901360 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902494 GO:1902531 GO:1902532 GO:1902679 GO:1903046 GO:1903506 GO:1903507 GO:1990234 GO:2000026 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-UBR

Protein Analysis

770

Amino Acids

85.51

Weight (kDa)

5.99

Isoelectric Point (pI)

48.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-UBR PF02207 122 - 188 7.7e-20 Putative zinc finger in N-recognin (UBR box)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1203
AccB1I GGYRCC 1 cut(s) 1063
AccI GTMKAC 1 cut(s) 5
AccII CGCG 4 cut(s) 154, 207, 267, 2052
AciI CCGC 8 cut(s) 152, 194, 205, 265, 355, 501, 519, 1028
AclWI GGATC 4 cut(s) 416, 480, 1992, 2005
AcoI YGGCCR 2 cut(s) 1162, 2072
AcsI RAATTY 3 cut(s) 1189, 1926, 2212
AcuI CTGAAG 3 cut(s) 344, 382, 1638
AcyI GRCGYC 1 cut(s) 1200
AdeI CACNNNGTG 1 cut(s) 1722
AfaI GTAC 2 cut(s) 1726, 1753
AfiI CCNNNNNNNGG 6 cut(s) 219, 316, 595, 1171, 1363, 1809
AflIII ACRYGT 1 cut(s) 2050
AjnI CCWGG 3 cut(s) 217, 310, 1554
AleI CACNNNNGTG 1 cut(s) 2270
AloI GAACNNNNNNTCC 2 cut(s) 1855, 1887
AluBI AGCT 9 cut(s) 371, 601, 658, 739, 801, 892, 1313, 1976, 2201
AluI AGCT 9 cut(s) 371, 601, 658, 739, 801, 892, 1313, 1976, 2201
Alw26I GTCTC 1 cut(s) 280
AlwI GGATC 4 cut(s) 416, 480, 1992, 2005
AoxI GGCC 6 cut(s) 210, 328, 617, 1162, 1351, 2072
ApeKI GCWGC 6 cut(s) 352, 710, 1030, 1114, 1276, 2261
ApoI RAATTY 3 cut(s) 1189, 1926, 2212
ArsI GACNNNNNNTTYG 2 cut(s) 330, 362
AseI ATTAAT 1 cut(s) 1949
Asp700I GAANNNNTTC 1 cut(s) 1224
AspA2I CCTAGG 1 cut(s) 692
AspLEI GCGC 1 cut(s) 543
AspS9I GGNCC 5 cut(s) 222, 328, 617, 1006, 2027
AsuC2I CCSGG 1 cut(s) 683
AsuHPI GGTGA 6 cut(s) 238, 533, 601, 862, 1166, 2015
AvaII GGWCC 3 cut(s) 222, 1006, 2027
AvrII CCTAGG 1 cut(s) 692
BalI TGGCCA 1 cut(s) 2074
BamHI GGATCC 1 cut(s) 1997
BanI GGYRCC 1 cut(s) 1063
BauI CACGAG 4 cut(s) 42, 277, 896, 1720
BbsI GAAGAC 3 cut(s) 1164, 1482, 1860
BbvI GCAGC 6 cut(s) 364, 697, 1017, 1101, 1288, 2248
BccI CCATC 4 cut(s) 910, 1433, 1657, 2018
BceAI ACGGC 3 cut(s) 1149, 1177, 1562
BcgI CGANNNNNNTGC 3 cut(s) 1587, 1621, 2287
BciT130I CCWGG 3 cut(s) 219, 312, 1556
BciVI GTATCC 2 cut(s) 1035, 1638
BcnI CCSGG 1 cut(s) 683
BcoDI GTCTC 1 cut(s) 280
BfaI CTAG 5 cut(s) 693, 1118, 1458, 1755, 2202
BfmI CTRYAG 2 cut(s) 711, 2150
BfoI RGCGCY 1 cut(s) 544
BfuI GTATCC 2 cut(s) 1035, 1638
BisI GCNGC 6 cut(s) 353, 711, 1031, 1115, 1277, 2262
BlnI CCTAGG 1 cut(s) 692
BlpI GCTNAGC 1 cut(s) 797
BlsI GCNGC 6 cut(s) 354, 712, 1032, 1116, 1278, 2263
BmcAI AGTACT 1 cut(s) 1753
Bme1390I CCNGG 4 cut(s) 219, 312, 683, 1556
Bme18I GGWCC 3 cut(s) 222, 1006, 2027
BmgT120I GGNCC 5 cut(s) 222, 328, 617, 1006, 2027
BmiI GGNNCC 7 cut(s) 223, 464, 618, 924, 1065, 1999, 2029
BmrFI CCNGG 4 cut(s) 219, 312, 683, 1556
BmrI ACTGGG 1 cut(s) 83
BmsI GCATC 7 cut(s) 750, 865, 1101, 1393, 1613, 2032, 2307
BmuI ACTGGG 1 cut(s) 83
BoxI GACNNNNGTC 1 cut(s) 1549
BpiI GAAGAC 3 cut(s) 1164, 1482, 1860
BplI GAGNNNNNCTC 2 cut(s) 52, 84
Bpu1102I GCTNAGC 1 cut(s) 797
BpuMI CCSGG 1 cut(s) 683
Bsa29I ATCGAT 2 cut(s) 18, 1968
BsaHI GRCGYC 1 cut(s) 1200
BsaWI WCCGGW 1 cut(s) 2000
Bsc4I CCNNNNNNNGG 6 cut(s) 219, 316, 595, 1171, 1363, 1809
Bse118I RCCGGY 2 cut(s) 239, 497
Bse1I ACTGG 4 cut(s) 78, 162, 1842, 2075
Bse3DI GCAATG 1 cut(s) 2019
BseBI CCWGG 3 cut(s) 219, 312, 1556
BseCI ATCGAT 2 cut(s) 18, 1968
BseGI GGATG 6 cut(s) 1127, 1305, 1332, 1444, 1516, 2298
BseLI CCNNNNNNNGG 6 cut(s) 219, 316, 595, 1171, 1363, 1809
BseMI GCAATG 1 cut(s) 2019
BseMII CTCAG 3 cut(s) 98, 1323, 2048
BseNI ACTGG 4 cut(s) 78, 162, 1842, 2075
BseRI GAGGAG 3 cut(s) 611, 1379, 1382
BseX3I CGGCCG 1 cut(s) 1162
BseXI GCAGC 6 cut(s) 364, 697, 1017, 1101, 1288, 2248
BseYI CCCAGC 1 cut(s) 1702
BsgI GTGCAG 3 cut(s) 591, 802, 1596
Bsh1236I CGCG 4 cut(s) 154, 207, 267, 2052
Bsh1285I CGRYCG 1 cut(s) 1165
BshFI GGCC 6 cut(s) 212, 330, 619, 1164, 1353, 2074
BshNI GGYRCC 1 cut(s) 1063
BshVI ATCGAT 2 cut(s) 18, 1968
BsiEI CGRYCG 1 cut(s) 1165
BsiSI CCGG 4 cut(s) 240, 498, 682, 2001
BslFI GGGAC 3 cut(s) 393, 1537, 2013
BslI CCNNNNNNNGG 6 cut(s) 219, 316, 595, 1171, 1363, 1809
BsmAI GTCTC 1 cut(s) 280
BsmBI CGTCTC 1 cut(s) 280
BsmFI GGGAC 3 cut(s) 393, 1537, 2013
BsnI GGCC 6 cut(s) 212, 330, 619, 1164, 1353, 2074
Bsp1407I TGTACA 1 cut(s) 1724
Bsp143I GATC 6 cut(s) 46, 421, 472, 1969, 1997, 2156
Bsp1720I GCTNAGC 1 cut(s) 797
Bsp19I CCATGG 3 cut(s) 9, 1858, 2031
BspACI CCGC 8 cut(s) 152, 194, 205, 265, 355, 501, 519, 1028
BspANI GGCC 6 cut(s) 212, 330, 619, 1164, 1353, 2074
BspCNI CTCAG 3 cut(s) 97, 1322, 2049
BspDI ATCGAT 2 cut(s) 18, 1968
BspFNI CGCG 4 cut(s) 154, 207, 267, 2052
BspHI TCATGA 3 cut(s) 475, 1236, 1270
BspLI GGNNCC 7 cut(s) 223, 464, 618, 924, 1065, 1999, 2029
BspMAI CTGCAG 1 cut(s) 715
BspPI GGATC 4 cut(s) 416, 480, 1992, 2005
BspT107I GGYRCC 1 cut(s) 1063
BsrDI GCAATG 1 cut(s) 2019
BsrFI RCCGGY 2 cut(s) 239, 497
BsrGI TGTACA 1 cut(s) 1724
BsrI ACTGG 4 cut(s) 78, 162, 1842, 2075
BssAI RCCGGY 2 cut(s) 239, 497
BssMI GATC 6 cut(s) 46, 421, 472, 1969, 1997, 2156
BssNI GRCGYC 1 cut(s) 1200
BssSI CACGAG 4 cut(s) 42, 277, 896, 1720
BssT1I CCWWGG 5 cut(s) 9, 544, 692, 1858, 2031
Bst2BI CACGAG 4 cut(s) 42, 277, 896, 1720
Bst2UI CCWGG 3 cut(s) 219, 312, 1556
Bst4CI ACNGT 5 cut(s) 234, 613, 976, 1063, 1107
Bst6I CTCTTC 1 cut(s) 1784
BstACI GRCGYC 1 cut(s) 1200
BstAPI GCANNNNNTGC 2 cut(s) 411, 1574
BstAUI TGTACA 1 cut(s) 1724
BstC8I GCNNGC 4 cut(s) 66, 603, 679, 2054
BstDEI CTNAG 6 cut(s) 84, 797, 1309, 1526, 2038, 2057
BstDSI CCRYGG 5 cut(s) 9, 612, 1858, 2031, 2232
BstEII GGTNACC 1 cut(s) 1174
BstENI CCTNNNNNAGG 1 cut(s) 593
BstF5I GGATG 6 cut(s) 1127, 1305, 1332, 1444, 1516, 2298
BstFNI CGCG 4 cut(s) 154, 207, 267, 2052
BstH2I RGCGCY 1 cut(s) 544
BstHHI GCGC 1 cut(s) 543
BstKTI GATC 6 cut(s) 49, 424, 475, 1972, 2000, 2159
BstMAI GTCTC 1 cut(s) 280
BstMBI GATC 6 cut(s) 46, 421, 472, 1969, 1997, 2156
BstMCI CGRYCG 1 cut(s) 1165
BstMWI GCNNNNNNNGC 3 cut(s) 411, 1574, 2207
BstNI CCWGG 3 cut(s) 219, 312, 1556
BstNSI RCATGY 4 cut(s) 414, 1535, 1572, 1602
BstPAI GACNNNNGTC 1 cut(s) 1549
BstPI GGTNACC 1 cut(s) 1174
BstSCI CCNGG 4 cut(s) 217, 310, 681, 1554
BstSFI CTRYAG 2 cut(s) 711, 2150
BstUI CGCG 4 cut(s) 154, 207, 267, 2052
BstV1I GCAGC 6 cut(s) 364, 697, 1017, 1101, 1288, 2248
BstV2I GAAGAC 3 cut(s) 1164, 1482, 1860
BstX2I RGATCY 1 cut(s) 1997
BstXI CCANNNNNNTGG 1 cut(s) 2272
BstYI RGATCY 1 cut(s) 1997
BstZI CGGCCG 1 cut(s) 1162
Bsu15I ATCGAT 2 cut(s) 18, 1968
BsuI GTATCC 2 cut(s) 1035, 1638
BsuRI GGCC 6 cut(s) 212, 330, 619, 1164, 1353, 2074
BsuTUI ATCGAT 2 cut(s) 18, 1968
BtgI CCRYGG 5 cut(s) 9, 612, 1858, 2031, 2232
BtgZI GCGATG 1 cut(s) 2114
BtsCI GGATG 6 cut(s) 1127, 1305, 1332, 1444, 1516, 2298
BtsI GCAGTG 3 cut(s) 628, 790, 1584
BtsIMutI CAGTG 3 cut(s) 628, 790, 1584
Cac8I GCNNGC 4 cut(s) 66, 603, 679, 2054
CciI TCATGA 3 cut(s) 475, 1236, 1270
CfoI GCGC 1 cut(s) 543
Cfr10I RCCGGY 2 cut(s) 239, 497
Cfr13I GGNCC 5 cut(s) 222, 328, 617, 1006, 2027
ClaI ATCGAT 2 cut(s) 18, 1968
CseI GACGC 1 cut(s) 262
Csp6I GTAC 2 cut(s) 1725, 1752
CspCI CAANNNNNGTGG 2 cut(s) 594, 629
CviQI GTAC 2 cut(s) 1725, 1752
DdeI CTNAG 6 cut(s) 84, 797, 1309, 1526, 2038, 2057
DpnI GATC 6 cut(s) 48, 423, 474, 1971, 1999, 2158
DpnII GATC 6 cut(s) 46, 421, 472, 1969, 1997, 2156
DraIII CACNNNGTG 1 cut(s) 1722
EaeI YGGCCR 2 cut(s) 1162, 2072
EagI CGGCCG 1 cut(s) 1162
Eam1104I CTCTTC 1 cut(s) 1784
EarI CTCTTC 1 cut(s) 1784
EclXI CGGCCG 1 cut(s) 1162
Eco130I CCWWGG 5 cut(s) 9, 544, 692, 1858, 2031
Eco147I AGGCCT 1 cut(s) 1353
Eco32I GATATC 1 cut(s) 16
Eco47I GGWCC 3 cut(s) 222, 1006, 2027
Eco52I CGGCCG 1 cut(s) 1162
Eco57I CTGAAG 3 cut(s) 344, 382, 1638
Eco91I GGTNACC 1 cut(s) 1174
EcoNI CCTNNNNNAGG 1 cut(s) 593
EcoO109I RGGNCCY 3 cut(s) 328, 617, 1006
EcoO65I GGTNACC 1 cut(s) 1174
EcoRII CCWGG 3 cut(s) 217, 310, 1554
EcoRV GATATC 1 cut(s) 16
EcoT14I CCWWGG 5 cut(s) 9, 544, 692, 1858, 2031
EcoT22I ATGCAT 1 cut(s) 1386
ErhI CCWWGG 5 cut(s) 9, 544, 692, 1858, 2031
Esp3I CGTCTC 1 cut(s) 280
FalI AAGNNNNNCTT 2 cut(s) 942, 974
FaqI GGGAC 3 cut(s) 393, 1537, 2013
FauI CCCGC 1 cut(s) 348
FauNDI CATATG 1 cut(s) 2086
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 6 cut(s) 353, 711, 1031, 1115, 1277, 2262
FokI GGATG 6 cut(s) 1134, 1312, 1339, 1451, 1523, 2285
Fsp4HI GCNGC 6 cut(s) 353, 711, 1031, 1115, 1277, 2262
FspBI CTAG 5 cut(s) 693, 1118, 1458, 1755, 2202
GlaI GCGC 1 cut(s) 542
GluI GCNGC 6 cut(s) 353, 711, 1031, 1115, 1277, 2262
GsaI CCCAGC 1 cut(s) 1706
HaeII RGCGCY 1 cut(s) 544
HaeIII GGCC 6 cut(s) 212, 330, 619, 1164, 1353, 2074
HapII CCGG 4 cut(s) 240, 498, 682, 2001
HgaI GACGC 1 cut(s) 262
HhaI GCGC 1 cut(s) 543
Hin1I GRCGYC 1 cut(s) 1200
Hin6I GCGC 1 cut(s) 541
HinP1I GCGC 1 cut(s) 541
HincII GTYRAC 3 cut(s) 832, 1668, 1909
HindII GTYRAC 3 cut(s) 832, 1668, 1909
HinfI GANTC 7 cut(s) 20, 32, 51, 88, 1240, 1330, 2189
HpaI GTTAAC 1 cut(s) 1668
HpaII CCGG 4 cut(s) 240, 498, 682, 2001
HphI GGTGA 6 cut(s) 238, 533, 601, 862, 1166, 2015
Hpy166II GTNNAC 7 cut(s) 6, 832, 1169, 1668, 1725, 1909, 2116
Hpy188I TCNGA 9 cut(s) 31, 60, 87, 324, 670, 1618, 1897, 2068, 2257
Hpy8I GTNNAC 7 cut(s) 6, 832, 1169, 1668, 1725, 1909, 2116
Hpy99I CGWCG 1 cut(s) 1612
HpyAV CCTTC 7 cut(s) 36, 319, 406, 599, 718, 1430, 2160
HpyCH4III ACNGT 5 cut(s) 234, 613, 976, 1063, 1107
HpyCH4IV ACGT 2 cut(s) 1200, 1610
HpyF10VI GCNNNNNNNGC 3 cut(s) 411, 1574, 2207
HpyF3I CTNAG 6 cut(s) 84, 797, 1309, 1526, 2038, 2057
HpySE526I ACGT 2 cut(s) 1200, 1610
Hsp92I GRCGYC 1 cut(s) 1200
HspAI GCGC 1 cut(s) 541
KspAI GTTAAC 1 cut(s) 1668
Kzo9I GATC 6 cut(s) 46, 421, 472, 1969, 1997, 2156
LmnI GCTCC 8 cut(s) 368, 598, 897, 909, 922, 1442, 1770, 2295
Lsp1109I GCAGC 6 cut(s) 364, 697, 1017, 1101, 1288, 2248
LweI GCATC 7 cut(s) 750, 865, 1101, 1393, 1613, 2032, 2307
MaeI CTAG 5 cut(s) 693, 1118, 1458, 1755, 2202
MaeII ACGT 2 cut(s) 1200, 1610
MaeIII GTNAC 9 cut(s) 147, 244, 305, 526, 697, 1174, 1201, 1264, 1460
MalI GATC 6 cut(s) 48, 423, 474, 1971, 1999, 2158
MboI GATC 6 cut(s) 46, 421, 472, 1969, 1997, 2156
MfeI CAATTG 1 cut(s) 1410
MflI RGATCY 1 cut(s) 1997
MlsI TGGCCA 1 cut(s) 2074
MluI ACGCGT 1 cut(s) 2050
MluNI TGGCCA 1 cut(s) 2074
MlyI GAGTC 1 cut(s) 1249
MmeI TCCRAC 2 cut(s) 449, 2091
Mox20I TGGCCA 1 cut(s) 2074
Mph1103I ATGCAT 1 cut(s) 1386
MroXI GAANNNNTTC 1 cut(s) 1224
MscI TGGCCA 1 cut(s) 2074
MseI TTAA 7 cut(s) 129, 453, 960, 1073, 1667, 1949, 2162
MslI CAYNNNNRTG 4 cut(s) 567, 1597, 1675, 2270
Msp20I TGGCCA 1 cut(s) 2074
MspA1I CMGCKG 2 cut(s) 355, 1030
MspI CCGG 4 cut(s) 240, 498, 682, 2001
MspR9I CCNGG 4 cut(s) 219, 312, 683, 1556
MunI CAATTG 1 cut(s) 1410
MvaI CCWGG 3 cut(s) 219, 312, 1556
MvnI CGCG 4 cut(s) 154, 207, 267, 2052
MwoI GCNNNNNNNGC 3 cut(s) 411, 1574, 2207
NciI CCSGG 1 cut(s) 683
NcoI CCATGG 3 cut(s) 9, 1858, 2031
NdeI CATATG 1 cut(s) 2086
NdeII GATC 6 cut(s) 46, 421, 472, 1969, 1997, 2156
NlaIV GGNNCC 7 cut(s) 223, 464, 618, 924, 1065, 1999, 2029
NmeAIII GCCGAG 1 cut(s) 1467
NmuCI GTSAC 4 cut(s) 244, 305, 1201, 1460
NsiI ATGCAT 1 cut(s) 1386
NspI RCATGY 4 cut(s) 414, 1535, 1572, 1602
OliI CACNNNNGTG 1 cut(s) 2270
PagI TCATGA 3 cut(s) 475, 1236, 1270
PceI AGGCCT 1 cut(s) 1353
PdmI GAANNNNTTC 1 cut(s) 1224
PfeI GAWTC 6 cut(s) 20, 32, 51, 88, 1330, 2189
PkrI GCNGC 6 cut(s) 354, 712, 1032, 1116, 1278, 2263
PleI GAGTC 1 cut(s) 1248
PpsI GAGTC 1 cut(s) 1248
PpuMI RGGWCCY 1 cut(s) 1006
PshAI GACNNNNGTC 1 cut(s) 1549
PshBI ATTAAT 1 cut(s) 1949
Psp5II RGGWCCY 1 cut(s) 1006
Psp6I CCWGG 3 cut(s) 217, 310, 1554
PspEI GGTNACC 1 cut(s) 1174
PspFI CCCAGC 1 cut(s) 1702
PspGI CCWGG 3 cut(s) 217, 310, 1554
PspN4I GGNNCC 7 cut(s) 223, 464, 618, 924, 1065, 1999, 2029
PspPI GGNCC 5 cut(s) 222, 328, 617, 1006, 2027
PspPPI RGGWCCY 1 cut(s) 1006
PsrI GAACNNNNNNTAC 2 cut(s) 183, 215
PstI CTGCAG 1 cut(s) 715
PsuI RGATCY 1 cut(s) 1997
RsaI GTAC 2 cut(s) 1726, 1753
RsaNI GTAC 2 cut(s) 1725, 1752
RseI CAYNNNNRTG 4 cut(s) 567, 1597, 1675, 2270
SaqAI TTAA 7 cut(s) 129, 453, 960, 1073, 1667, 1949, 2162
SatI GCNGC 6 cut(s) 353, 711, 1031, 1115, 1277, 2262
Sau3AI GATC 6 cut(s) 46, 421, 472, 1969, 1997, 2156
Sau96I GGNCC 5 cut(s) 222, 328, 617, 1006, 2027
ScaI AGTACT 1 cut(s) 1753
SchI GAGTC 1 cut(s) 1249
ScrFI CCNGG 4 cut(s) 219, 312, 683, 1556
SfaNI GCATC 7 cut(s) 750, 865, 1101, 1393, 1613, 2032, 2307
SfcI CTRYAG 2 cut(s) 711, 2150
SinI GGWCC 3 cut(s) 222, 1006, 2027
SmiMI CAYNNNNRTG 4 cut(s) 567, 1597, 1675, 2270
SseBI AGGCCT 1 cut(s) 1353
SsiI CCGC 8 cut(s) 152, 194, 205, 265, 355, 501, 519, 1028
SspI AATATT 1 cut(s) 2250
SspMI CTAG 5 cut(s) 693, 1118, 1458, 1755, 2202
StuI AGGCCT 1 cut(s) 1353
StyD4I CCNGG 4 cut(s) 217, 310, 681, 1554
StyI CCWWGG 5 cut(s) 9, 544, 692, 1858, 2031
TaaI ACNGT 5 cut(s) 234, 613, 976, 1063, 1107
TaiI ACGT 2 cut(s) 1203, 1613
TaqI TCGA 7 cut(s) 18, 49, 809, 1109, 1607, 1692, 1968
TatI WGTACW 2 cut(s) 1724, 1751
TfiI GAWTC 6 cut(s) 20, 32, 51, 88, 1330, 2189
Tru1I TTAA 7 cut(s) 129, 453, 960, 1073, 1667, 1949, 2162
Tru9I TTAA 7 cut(s) 129, 453, 960, 1073, 1667, 1949, 2162
TscAI CASTG 3 cut(s) 635, 790, 1584
TseFI GTSAC 4 cut(s) 244, 305, 1201, 1460
TseI GCWGC 6 cut(s) 352, 710, 1030, 1114, 1276, 2261
Tsp45I GTSAC 4 cut(s) 244, 305, 1201, 1460
TspDTI ATGAA 8 cut(s) 86, 266, 1112, 1343, 1412, 1581, 1721, 1863
TspGWI ACGGA 2 cut(s) 435, 2249
TspRI CASTG 3 cut(s) 635, 790, 1584
VpaK11BI GGWCC 3 cut(s) 222, 1006, 2027
VspI ATTAAT 1 cut(s) 1949
XagI CCTNNNNNAGG 1 cut(s) 593
XapI RAATTY 3 cut(s) 1189, 1926, 2212
XceI RCATGY 4 cut(s) 414, 1535, 1572, 1602
XcmI CCANNNNNNNNNTGG 2 cut(s) 1865, 2239
XmaJI CCTAGG 1 cut(s) 692
XmiI GTMKAC 1 cut(s) 5
XmnI GAANNNNTTC 1 cut(s) 1224
XspI CTAG 5 cut(s) 693, 1118, 1458, 1755, 2202
ZraI GACGTC 1 cut(s) 1201
ZrmI AGTACT 1 cut(s) 1753
Zsp2I ATGCAT 1 cut(s) 1386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.