Rmu_sc0003332.1_g000005

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003332.1
OG0007182
Physical Location & Seq
Reverse (-)
18096 .. 28035
9940 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003332.1_g000005.1.cds

Sequence Viewer

Length: 6477 bp
atggatatcgattccccttctgaatctctccctcgtgatcgaatcgttcagaggcttgctcgagtgggagtgcctcagaatcttcttcatctgcatcaacttggtttagttgcttttgttaaaaataacaagcagttgttaccgcgactggtttctgctaccttacctcctgatgaggaagtagcggaagttctccgcgaggccaaaccagggtcgaagaaacaatcagccggtgtcaccatgaaacttcaattccgcgagagcatctcgtggctcaagtggctgatgtttgagagtgaccctggcctggttctcaagggcctgtccaaaatgagtgtcggccagcggggcatttgtggagctgtttggggacataatgatatagccttcaggtgcaaaacatgcgaacatgatccgacctgtgcaatttgcgttccgtgttttaagaatgggaaccacaaggatcatgattactccattatctacaccggcggtggctgttgtgattgcggtgacgttacagcgtggaaacgccaaggcttttgctccaagcataaaggtgcagagcagatacagccccttccagaggagcttgcagtcaccgtggggcctgttcttgactgcctctttgcctattggaagaacaagctacttatttctgaaactgcctgccgggaaaaccctagactaactgaccttgctgcagagcgtaagaaggttgcaaatgagctaacctattttgtggttgagatgcttctggacttttgcaagtgcagtgagagtttgcttagctttgtatcaaagaagattttatcatcagttgacgttttggggattttggtgagggcagagaggtttttgagtgatgctgttgtgaaaaagctccacgagttgctcctgaagttgatgggggagcctgttttcaaatatgagtttgccaaagtatttcttaattattacccaactgttgttagtgaagccataaaggagttcaatgaccttactttgaaaaagtatccgctgctctctatattctctgtgcaaatcctcacggtgccaactttaacaccccgtcttgtgaaagaaatgaacctactgtcgatgctgttagaacgtgtggaagatattttcaattcctgtgctggtgaagacggccgtttacaggttaccaagtggtcaaatttgtatgacgtcactattcgtgtgattgaagattttcggtttgtcatgagtcatgctattgtgccaaaatatgtaactcatgagcagcaagatattgcgagaacttggatgaggctctcagcttttgtgcaagggatgaatccacaaaagagggaaacaggcctccatattgaggaagagaatgacaatatgcatctgccattcgttctgggtcattcaattgccaaccttcattctttgttggtggatggagcattttctgttgctagtgacaacacagaagaagacttatttctcagcacaaataagcaagatatggatgacacagatagcctaagatatgcaaaagtaggacggctgtcccaggaaagttctgcatgtagtgcagtggggagtagttcatttgcatgtgcatcgacgttttctgaagatacatcaaatgctcttttgaggataccatcatctgtgatatggttaacatttgagtgtttgagggctatcgagaattggctgggagttgataacacctcgggtacgattcatcatgtatcatcttcaagtactagcaatttctctgggagcaatttttcagcattgaagagaacattatccaaaattaggaaaggcaaaaacatttttggtagatttgccagttcaagtgaagaccatgggaagcatggttcatcacatttacatagtagttctgatatgagtgttgacttccagaatggcaaaatttcagggcaggaaaataaattaatggttctggacgaaatcgatcaagctaatggctgtaattcgtcaggatccggtgatagtgcaatggaaatagatggtcccatggacttagaagctctacgagtgctgagttcgtccgactggccagacataacatatgatgttagttcacaggatatatctgttcacattcccttacatcgcttccttgcgttgcttctacagagatcattaagaaggtgttttggcgaagtgccagattctgttagagctagttctgcaaatttatcatcatctatttccacagacttttttggtaatattctcggaggctgccacccgtgtgggttttctgcctttgttatggagcatccccttcgtataagagtattttgtgctgaggttcacgctggaatgtggcggaaaaatggggatgctgccccattaacttgtgagtggtatcgttcagttcgctggtcagaacagtttgtggagcttgatctatttctgcttcagtgctgtgctgccttggctccagctgatctttatatcaagagaatcatagagcgctttggcttgtcaagctacctttctctgaaccttgagcgctctagtgaatatgaaccagttctagtgcaggaaatgctcactcttattatacaaataatcaaagaaaggcgattctgtgggttaactaaagctgaaagtgtgaaaagagaattgattcataagttgagcatcgcagatgctactcacagtcaattggttaagtctcttcctcgtgatcttaccaagtatgaacagcttaatgaaattttggacacagttgctgtgtattccaatccctctggctttaaccaggggacttattcactacaatggacattttgggaggagctggatttgtactaccatcgttggaactcaagggatctacaagctgcagaagaaagatacttacgtttccgtggcgtttctgcattgacgagtcagctacccaggtggactaagatttatcctcctttcagggggttagctagaatagggacctgtaaaaccgtcctccaaattgttcgtgcggtgctgttttatgctgtattttctgataaatcggttgagtcgcgagcgcctgatggggtcctcttaactgcattgcacttgctctcattagcattagacatttgttctcaacataaagaatctggtgaccattcctgttatgatggagatttcattccagtgcttgcttttgcctgtgaagaaattaatgaaggactctattttgaggctggtcaacaaagcttgttgtcacttcttgttattttgatgaggatgtattcgaaagaggggttagataattcagaagatggaagttgcaacctttcttctttaattggaaacttactgaagaagtttgcatttattgattgtgaatgtatgaccaaactgcaggtacttgcacctgagttggttagtcatgtaacttttccaaatagtgatacagttacctcaggttcagcttctgattctgagaagcgaaaggcaaaagccagagagagacaggctgccatactggaaaaaatgagagccgagcaatcaaagtttttgagtagtattgattcctctgtggatgatggttcagaagctgaacaaaaggatgttgaatctgatgttgaagataattcagaagagcctgcacaagttgtttgctcgctctgccatgatccgaattccaagaatcctatttctttcttggttcttcttcaaaagtctaggcttttgagtttcattgatagaggtccactatcatgggaacaacctcgctccgtagacaaggagcatgttgttaaaaccaaagatgaggtgattgatcaatctggcataagcagattgtttagtggttcaggatttgtttcatcgtctcatgtaagacagttggtgtcggaacactacaacaaacaaaaggtgactggtgaatctggaacaagtacactgtcaagtggttcaggagtggtgtcgcctcatcatctaaggcagttggttgagaaggcagtcactgagtttgcccgacatgggagacctagggatgttgatgcctttctagaagttctgaaggggcggttccatgacttaagaaatttccgagtgccatgtgaatcaaatgatgaaagggaaagtactttatatgcctttgagacaatggaagaagatatgtacttctctgtccgaagagaattgcacgataaactgcattctaaacttacagaagataagaagtgtacagctgctgaagggggtcgagagaatacagagcatactgaatttatgttgcttggaaactatatagctgctctttctagagagagaagagaagatccttcatcttctgaaagtactcctaatgataaggggccagtagagtgttctcgcctgtcagcatatgatggatttggccctgcagattgtgatggaatttttctttcttcctgtgggcatgctgtgcatcaggaatgtcttgatcgttatttgtcttccctgaaggaaaggtatctcaggagaattgtttttgaaggaggacatattgtagatccagacaagggtgaatttctttgtcctgtatgccgtcgacttgcaaattcagtcttgcctacattaccaagtgagttccagaaggttcgcaaagagcctatggatacagatgttagttcatcacatgttactagtccatcatgcaaaccaggcgaaggaattagttcttttcagcaaggcctggctctcttgcaatctgcagcaaatgtagctgggaaggtcggcacgctcaaaggtttccccctgcatagacgtgaacaaatgagctcaaatcttgaaccaatctcacgtctgctttctaagatgtatttcccaactgatctagataagatatcaggatctgacagggtcagccacccaatgcttatgtgggacttaattaagtactctctactgtcaatggagattgcttctcgttctggggggaaatatgctgcaccaagctacagccttaatgccttgtataaggaacttgaatcctcgagcagattcatattgtccttgttgctgaaacttgtccaaaacacgtgcaagaattctcttcatgtgcttcaaagatttatagcaattcagagctttgcagaatctacgtgctttggagtttccatagatcatggaagcaaaacatcaggacaagttgttatgctgcgtatcttggaacatgttgataatccagtagcatatcctgatttccagttctggaatcgagcttctgatcctgtgcttgctcgtgatcctttttcttcattgatgtgggttctattttgtcttccatatcgatttttatcatgtgaagattccttgctatccctggtgcacgttttttatgttgtttctgtggttcagggtattgctacatattttgggaaaaatcagtgcgacagtagtggattaggtgttggtgattgcctgattactgacatcagcaagcttatgaatgaatttggatccgctcggcaatattttgtatcaaactatatagactcttcttctaatattaagaatatagttcgtagcttgactttcccttatttgcggaggtgtgcattgttgttgaagctactcgaatcctatgcccaagtgccattttgtgaaaggtataatgcgttggatagatcacgtgccactagtgactcaatggatgccacttatgtgccactggttgagctcaatgaaattcaagagatagaaaccctgctcaagattccttctttggatgttattcttaaggatgaagtggtgcgttcattagctcataaatggttctgtcattttggcaaagagtttgaggacaaaaggtttcaaggcagtatacactgcaacccttcagttccattccagttgatgcgtctgccccgagtttaccaggatctattgcagcggtatataaagcagcggtgtcgtgactgcaataacatacttgatgaacctgcactgtgcctactttgtggtaggttatgctctccaagctggaaatcatgctgcagggaaagtggatgccaaactcatgcagtggcttgtggttctggtactggggtctttctgttgattaggagaacaacaatactgctgcaaagatctgcacggcaggctccctggccatcaccctacttggatgcttttggtgaagaggacattgaaatgcagagaggaaagccactgttcttgaatgaggagcgttatgcagctttaacttatttggttgcttctcatggcctagatcgaatttggattataagattgatgtcaacgggatggcaaagaactctagttaacagcagggaatatccaatgctaacaatcgatcttaatcttaatctttggaattttcactgtatattgagatgcttggagtttcctttccagagtttcgactattggttctacagcagactagtcttttgccaggttgtttctccagttttcaaggttgtacagttcaatgtgatgctggaagctgaagcatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000151 GO:0000280 GO:0001101 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006259 GO:0006310 GO:0006325 GO:0006342 GO:0006355 GO:0006464 GO:0006508 GO:0006511 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0007049 GO:0007127 GO:0007131 GO:0007140 GO:0007141 GO:0007276 GO:0007283 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009605 GO:0009607 GO:0009620 GO:0009719 GO:0009725 GO:0009737 GO:0009889 GO:0009890 GO:0009892 GO:0009894 GO:0009966 GO:0009968 GO:0009987 GO:0010029 GO:0010033 GO:0010243 GO:0010468 GO:0010498 GO:0010528 GO:0010529 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010646 GO:0010648 GO:0016043 GO:0016458 GO:0016567 GO:0016569 GO:0016570 GO:0016574 GO:0016597 GO:0016740 GO:0019216 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0019941 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0023051 GO:0023057 GO:0030163 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031406 GO:0032006 GO:0032007 GO:0032446 GO:0032501 GO:0032504 GO:0032991 GO:0033522 GO:0033993 GO:0034641 GO:0035825 GO:0036094 GO:0036211 GO:0040029 GO:0042221 GO:0043161 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043177 GO:0043200 GO:0043201 GO:0043207 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0044703 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048232 GO:0048285 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0048609 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0050994 GO:0051171 GO:0051172 GO:0051239 GO:0051252 GO:0051253 GO:0051276 GO:0051321 GO:0051603 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0061630 GO:0061659 GO:0061982 GO:0065007 GO:0070647 GO:0070728 GO:0070887 GO:0071229 GO:0071230 GO:0071233 GO:0071310 GO:0071417 GO:0071495 GO:0071596 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097305 GO:0098542 GO:0140013 GO:0140096 GO:1900140 GO:1901360 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902494 GO:1902531 GO:1902532 GO:1902679 GO:1903046 GO:1903506 GO:1903507 GO:1990234 GO:2000026 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-UBR

Protein Analysis

2158

Amino Acids

242.83

Weight (kDa)

6.11

Isoelectric Point (pI)

49.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 6245
AatII GACGTC 1 cut(s) 1194
Acc36I ACCTGC 2 cut(s) 3361, 5947
AccB1I GGYRCC 1 cut(s) 1054
AccBSI CCGCTC 1 cut(s) 5462
AccI GTMKAC 3 cut(s) 3750, 4546, 5821
AccII CGCG 4 cut(s) 145, 198, 258, 3043
AcoI YGGCCR 4 cut(s) 340, 1153, 2063, 6107
AcuI CTGAAG 9 cut(s) 373, 920, 1629, 2417, 3347, 4052, 4230, 4478, 5820
AcvI CACGTG 2 cut(s) 5037, 5630
AcyI GRCGYC 1 cut(s) 1191
AfeI AGCGCT 2 cut(s) 2489, 2528
AflII CTTAAG 2 cut(s) 4051, 5735
AflIII ACRYGT 4 cut(s) 1114, 4633, 5034, 5170
AhlI ACTAGT 3 cut(s) 4640, 5636, 6403
AjiI CACGTC 2 cut(s) 4763, 4799
AleI CACNNNNGTG 2 cut(s) 2262, 5660
AloI GAACNNNNNNTCC 2 cut(s) 1846, 1878
Alw21I GWGCWC 3 cut(s) 4778, 5328, 5679
Alw26I GTCTC 5 cut(s) 2697, 3472, 3846, 3991, 4109
Alw44I GTGCAC 1 cut(s) 5324
AlwNI CAGNNNCTG 7 cut(s) 2183, 2750, 3443, 3566, 3977, 4208, 4850
Ama87I CYCGRG 4 cut(s) 60, 1711, 4990, 5865
Aor51HI AGCGCT 2 cut(s) 2489, 2528
ApaLI GTGCAC 1 cut(s) 5324
ArsI GACNNNNNNTTYG 6 cut(s) 321, 353, 611, 643, 6393, 6425
AseI ATTAAT 2 cut(s) 1940, 3186
Asp700I GAANNNNTTC 4 cut(s) 1215, 2547, 2643, 4672
AspA2I CCTAGG 1 cut(s) 4001
AspLEI GCGC 3 cut(s) 2490, 2529, 3049
AspS9I GGNCC 8 cut(s) 319, 608, 2018, 2967, 3058, 3719, 4331, 4373
AsuC2I CCSGG 1 cut(s) 674
AsuII TTCGAA 1 cut(s) 3260
AvaI CYCGRG 4 cut(s) 60, 1711, 4990, 5865
AvaII GGWCC 4 cut(s) 2018, 2967, 3058, 3719
AvrII CCTAGG 1 cut(s) 4001
AxyI CCTNAGG 1 cut(s) 3430
BaeGI GKGCMC 1 cut(s) 5328
BaeI ACNNNNGTAYC 6 cut(s) 2866, 2899, 4605, 4638, 6030, 6063
BalI TGGCCA 2 cut(s) 2065, 6109
BamHI GGATCC 2 cut(s) 1988, 5456
BanI GGYRCC 1 cut(s) 1054
BanII GRGCYC 2 cut(s) 4778, 5679
BauI CACGAG 5 cut(s) 33, 268, 887, 2700, 5238
BbrPI CACGTG 2 cut(s) 5037, 5630
BbsI GAAGAC 5 cut(s) 1155, 1473, 1851, 4443, 5270
Bbv12I GWGCWC 3 cut(s) 4778, 5328, 5679
BbvCI CCTCAGC 1 cut(s) 2319
BceAI ACGGC 5 cut(s) 1140, 1168, 1553, 4527, 6110
BcgI CGANNNNNNTGC 8 cut(s) 1578, 1612, 2279, 2313, 5564, 5598, 5891, 5925
BciVI GTATCC 3 cut(s) 1026, 1629, 4606
BclI TGATCA 1 cut(s) 3790
BcnI CCSGG 1 cut(s) 674
BcoDI GTCTC 5 cut(s) 2697, 3472, 3846, 3991, 4109
BcuI ACTAGT 3 cut(s) 4640, 5636, 6403
BfmI CTRYAG 9 cut(s) 702, 2141, 2862, 3368, 4377, 4707, 4954, 5992, 6394
BfoI RGCGCY 3 cut(s) 2491, 2530, 3050
BfrI CTTAAG 2 cut(s) 4051, 5735
BfuAI ACCTGC 2 cut(s) 3361, 5947
BfuI GTATCC 3 cut(s) 1026, 1629, 4606
BglI GCCNNNNNGGC 1 cut(s) 348
BglII AGATCT 1 cut(s) 6086
BlnI CCTAGG 1 cut(s) 4001
BlpI GCTNAGC 1 cut(s) 788
BmcAI AGTACT 4 cut(s) 1744, 4099, 4315, 4895
Bme18I GGWCC 4 cut(s) 2018, 2967, 3058, 3719
BmeT110I CYCGRG 4 cut(s) 60, 1711, 4990, 5865
BmgBI CACGTC 2 cut(s) 4763, 4799
BmgT120I GGNCC 8 cut(s) 319, 608, 2018, 2967, 3058, 3719, 4331, 4373
BmrI ACTGGG 1 cut(s) 6051
BmuI ACTGGG 1 cut(s) 6051
BoxI GACNNNNGTC 1 cut(s) 1540
BpiI GAAGAC 5 cut(s) 1155, 1473, 1851, 4443, 5270
BplI GAGNNNNNCTC 4 cut(s) 43, 75, 251, 283
BpmI CTGGAG 2 cut(s) 2439, 6411
Bpu10I CCTNAGC 1 cut(s) 2319
Bpu1102I GCTNAGC 1 cut(s) 788
Bpu14I TTCGAA 1 cut(s) 3260
BpuEI CTTGAG 5 cut(s) 260, 299, 2543, 2830, 5693
BpuMI CCSGG 1 cut(s) 674
Bsa29I ATCGAT 4 cut(s) 9, 1959, 5287, 6312
BsaAI YACGTR 3 cut(s) 5037, 5100, 5630
BsaBI GATNNNNATC 2 cut(s) 5293, 6318
BsaHI GRCGYC 1 cut(s) 1191
BsaI GGTCTC 1 cut(s) 3991
BsaWI WCCGGW 1 cut(s) 1991
BsaXI ACNNNNNCTCC 2 cut(s) 151, 181
Bse118I RCCGGY 2 cut(s) 230, 488
Bse21I CCTNAGG 1 cut(s) 3430
Bse3DI GCAATG 2 cut(s) 2010, 3071
Bse8I GATNNNNATC 2 cut(s) 5293, 6318
BseCI ATCGAT 4 cut(s) 9, 1959, 5287, 6312
BseJI GATNNNNATC 2 cut(s) 5293, 6318
BseMI GCAATG 2 cut(s) 2010, 3071
BseRI GAGGAG 3 cut(s) 602, 2828, 6197
BseSI GKGCMC 1 cut(s) 5328
BseX3I CGGCCG 1 cut(s) 1153
BseYI CCCAGC 2 cut(s) 1693, 4721
BsgI GTGCAG 8 cut(s) 582, 793, 1587, 2576, 3600, 4929, 5925, 6075
Bsh1236I CGCG 4 cut(s) 145, 198, 258, 3043
Bsh1285I CGRYCG 1 cut(s) 1156
BshNI GGYRCC 1 cut(s) 1054
BshVI ATCGAT 4 cut(s) 9, 1959, 5287, 6312
BsiEI CGRYCG 1 cut(s) 1156
BsiHKAI GWGCWC 3 cut(s) 4778, 5328, 5679
BsiHKCI CYCGRG 4 cut(s) 60, 1711, 4990, 5865
BsiSI CCGG 4 cut(s) 231, 489, 673, 1992
BslFI GGGAC 6 cut(s) 384, 1528, 2004, 2797, 2980, 4895
BsmAI GTCTC 5 cut(s) 2697, 3472, 3846, 3991, 4109
BsmBI CGTCTC 1 cut(s) 3846
BsmFI GGGAC 6 cut(s) 384, 1528, 2004, 2797, 2980, 4895
BsmI GAATGC 1 cut(s) 4171
Bso31I GGTCTC 1 cut(s) 3991
BsoBI CYCGRG 4 cut(s) 60, 1711, 4990, 5865
Bsp119I TTCGAA 1 cut(s) 3260
Bsp1286I GDGCHC 3 cut(s) 4778, 5328, 5679
Bsp1407I TGTACA 2 cut(s) 4199, 6442
Bsp1720I GCTNAGC 1 cut(s) 788
Bsp19I CCATGG 2 cut(s) 1849, 2022
Bsp68I TCGCGA 1 cut(s) 3043
BspDI ATCGAT 4 cut(s) 9, 1959, 5287, 6312
BspFNI CGCG 4 cut(s) 145, 198, 258, 3043
BspHI TCATGA 3 cut(s) 466, 1227, 1261
BspMAI CTGCAG 6 cut(s) 706, 2866, 3372, 4381, 4711, 5996
BspMI ACCTGC 2 cut(s) 3361, 5947
BspQI GCTCTTC 1 cut(s) 3603
BspT104I TTCGAA 1 cut(s) 3260
BspT107I GGYRCC 1 cut(s) 1054
BspTI CTTAAG 2 cut(s) 4051, 5735
BspTNI GGTCTC 1 cut(s) 3991
BsrBI CCGCTC 1 cut(s) 5462
BsrDI GCAATG 2 cut(s) 2010, 3071
BsrFI RCCGGY 2 cut(s) 230, 488
BsrGI TGTACA 2 cut(s) 4199, 6442
BssAI RCCGGY 2 cut(s) 230, 488
BssNAI GTATAC 1 cut(s) 5822
BssNI GRCGYC 1 cut(s) 1191
BssSI CACGAG 5 cut(s) 33, 268, 887, 2700, 5238
BssT1I CCWWGG 5 cut(s) 535, 1849, 2022, 2448, 4001
Bst1107I GTATAC 1 cut(s) 5822
Bst2BI CACGAG 5 cut(s) 33, 268, 887, 2700, 5238
Bst6I CTCTTC 9 cut(s) 1353, 1775, 2700, 3603, 4144, 4282, 5055, 5500, 6132
BstACI GRCGYC 1 cut(s) 1191
BstAFI CTTAAG 2 cut(s) 4051, 5735
BstAPI GCANNNNNTGC 4 cut(s) 402, 1565, 2563, 4420
BstAUI TGTACA 2 cut(s) 4199, 6442
BstBAI YACGTR 3 cut(s) 5037, 5100, 5630
BstBI TTCGAA 1 cut(s) 3260
BstDSI CCRYGG 4 cut(s) 603, 1849, 2022, 2887
BstEII GGTNACC 2 cut(s) 1165, 3125
BstENI CCTNNNNNAGG 1 cut(s) 584
BstFNI CGCG 4 cut(s) 145, 198, 258, 3043
BstH2I RGCGCY 3 cut(s) 2491, 2530, 3050
BstHHI GCGC 3 cut(s) 2490, 2529, 3049
BstMAI GTCTC 5 cut(s) 2697, 3472, 3846, 3991, 4109
BstMCI CGRYCG 1 cut(s) 1156
BstNSI RCATGY 7 cut(s) 405, 1563, 1593, 3764, 4418, 4637, 5174
BstPAI GACNNNNGTC 1 cut(s) 1540
BstPI GGTNACC 2 cut(s) 1165, 3125
BstSFI CTRYAG 9 cut(s) 702, 2141, 2862, 3368, 4377, 4707, 4954, 5992, 6394
BstSLI GKGCMC 1 cut(s) 5328
BstUI CGCG 4 cut(s) 145, 198, 258, 3043
BstV2I GAAGAC 5 cut(s) 1155, 1473, 1851, 4443, 5270
BstX2I RGATCY 8 cut(s) 1988, 2851, 4294, 4507, 4847, 5456, 5878, 6086
BstXI CCANNNNNNTGG 2 cut(s) 2264, 3729
BstYI RGATCY 8 cut(s) 1988, 2851, 4294, 4507, 4847, 5456, 5878, 6086
BstZ17I GTATAC 1 cut(s) 5822
BstZI CGGCCG 1 cut(s) 1153
Bsu15I ATCGAT 4 cut(s) 9, 1959, 5287, 6312
Bsu36I CCTNAGG 1 cut(s) 3430
BsuI GTATCC 3 cut(s) 1026, 1629, 4606
BsuTUI ATCGAT 4 cut(s) 9, 1959, 5287, 6312
BtgI CCRYGG 4 cut(s) 603, 1849, 2022, 2887
BtgZI GCGATG 2 cut(s) 2105, 2644
BtrI CACGTC 2 cut(s) 4763, 4799
BtsI GCAGTG 4 cut(s) 781, 1575, 5824, 6027
BtuMI TCGCGA 1 cut(s) 3043
BveI ACCTGC 2 cut(s) 3361, 5947
CaiI CAGNNNCTG 7 cut(s) 2183, 2750, 3443, 3566, 3977, 4208, 4850
CciI TCATGA 3 cut(s) 466, 1227, 1261
CfoI GCGC 3 cut(s) 2490, 2529, 3049
Cfr10I RCCGGY 2 cut(s) 230, 488
Cfr13I GGNCC 8 cut(s) 319, 608, 2018, 2967, 3058, 3719, 4331, 4373
ClaI ATCGAT 4 cut(s) 9, 1959, 5287, 6312
CseI GACGC 1 cut(s) 5846
CspCI CAANNNNNGTGG 2 cut(s) 6156, 6191
EaeI YGGCCR 4 cut(s) 340, 1153, 2063, 6107
EagI CGGCCG 1 cut(s) 1153
Eam1104I CTCTTC 9 cut(s) 1353, 1775, 2700, 3603, 4144, 4282, 5055, 5500, 6132
EarI CTCTTC 9 cut(s) 1353, 1775, 2700, 3603, 4144, 4282, 5055, 5500, 6132
EciI GGCGGA 1 cut(s) 2356
Ecl136II GAGCTC 2 cut(s) 4776, 5677
EclXI CGGCCG 1 cut(s) 1153
Eco130I CCWWGG 5 cut(s) 535, 1849, 2022, 2448, 4001
Eco147I AGGCCT 2 cut(s) 1344, 4689
Eco24I GRGCYC 2 cut(s) 4778, 5679
Eco31I GGTCTC 1 cut(s) 3991
Eco32I GATATC 2 cut(s) 7, 4842
Eco47I GGWCC 4 cut(s) 2018, 2967, 3058, 3719
Eco47III AGCGCT 2 cut(s) 2489, 2528
Eco52I CGGCCG 1 cut(s) 1153
Eco53kI GAGCTC 2 cut(s) 4776, 5677
Eco57I CTGAAG 9 cut(s) 373, 920, 1629, 2417, 3347, 4052, 4230, 4478, 5820
Eco72I CACGTG 2 cut(s) 5037, 5630
Eco81I CCTNAGG 1 cut(s) 3430
Eco88I CYCGRG 4 cut(s) 60, 1711, 4990, 5865
Eco91I GGTNACC 2 cut(s) 1165, 3125
EcoICRI GAGCTC 2 cut(s) 4776, 5677
EcoNI CCTNNNNNAGG 1 cut(s) 584
EcoO109I RGGNCCY 4 cut(s) 319, 608, 2967, 3058
EcoO65I GGTNACC 2 cut(s) 1165, 3125
EcoRI GAATTC 2 cut(s) 3649, 5044
EcoRV GATATC 2 cut(s) 7, 4842
EcoT14I CCWWGG 5 cut(s) 535, 1849, 2022, 2448, 4001
EcoT22I ATGCAT 1 cut(s) 1377
EcoT38I GRGCYC 2 cut(s) 4778, 5679
ErhI CCWWGG 5 cut(s) 535, 1849, 2022, 2448, 4001
Esp3I CGTCTC 1 cut(s) 3846
FalI AAGNNNNNCTT 2 cut(s) 933, 965
FaqI GGGAC 6 cut(s) 384, 1528, 2004, 2797, 2980, 4895
FauI CCCGC 1 cut(s) 339
FauNDI CATATG 2 cut(s) 2077, 4360
FbaI TGATCA 1 cut(s) 3790
FblI GTMKAC 3 cut(s) 3750, 4546, 5821
FriOI GRGCYC 2 cut(s) 4778, 5679
GlaI GCGC 3 cut(s) 2489, 2528, 3048
GsaI CCCAGC 2 cut(s) 1697, 4725
GsuI CTGGAG 2 cut(s) 2439, 6411
HaeII RGCGCY 3 cut(s) 2491, 2530, 3050
HapII CCGG 4 cut(s) 231, 489, 673, 1992
HgaI GACGC 1 cut(s) 5846
HhaI GCGC 3 cut(s) 2490, 2529, 3049
Hin1I GRCGYC 1 cut(s) 1191
Hin6I GCGC 3 cut(s) 2488, 2527, 3047
HinP1I GCGC 3 cut(s) 2488, 2527, 3047
HincII GTYRAC 8 cut(s) 823, 1659, 1900, 2613, 3215, 4547, 6258, 6283
HindII GTYRAC 8 cut(s) 823, 1659, 1900, 2613, 3215, 4547, 6258, 6283
HindIII AAGCTT 2 cut(s) 3220, 5438
HpaI GTTAAC 3 cut(s) 1659, 2613, 6283
HpaII CCGG 4 cut(s) 231, 489, 673, 1992
Hpy99I CGWCG 2 cut(s) 1603, 4548
Hsp92I GRCGYC 1 cut(s) 1191
HspAI GCGC 3 cut(s) 2488, 2527, 3047
Ksp22I TGATCA 1 cut(s) 3790
KspAI GTTAAC 3 cut(s) 1659, 2613, 6283
LguI GCTCTTC 1 cut(s) 3603
MbiI CCGCTC 1 cut(s) 5462
MfeI CAATTG 2 cut(s) 1401, 2681
MflI RGATCY 8 cut(s) 1988, 2851, 4294, 4507, 4847, 5456, 5878, 6086
MhlI GDGCHC 3 cut(s) 4778, 5328, 5679
MlsI TGGCCA 2 cut(s) 2065, 6109
MluNI TGGCCA 2 cut(s) 2065, 6109
MlyI GAGTC 6 cut(s) 1240, 2917, 3047, 3189, 5486, 5636
MmeI TCCRAC 5 cut(s) 440, 2082, 2819, 3843, 5598
Mox20I TGGCCA 2 cut(s) 2065, 6109
Mph1103I ATGCAT 1 cut(s) 1377
MroXI GAANNNNTTC 4 cut(s) 1215, 2547, 2643, 4672
MscI TGGCCA 2 cut(s) 2065, 6109
Msp20I TGGCCA 2 cut(s) 2065, 6109
MspA1I CMGCKG 6 cut(s) 346, 1021, 2459, 4205, 5890, 5905
MspCI CTTAAG 2 cut(s) 4051, 5735
MspI CCGG 4 cut(s) 231, 489, 673, 1992
MunI CAATTG 2 cut(s) 1401, 2681
Mva1269I GAATGC 1 cut(s) 4171
MvnI CGCG 4 cut(s) 145, 198, 258, 3043
NciI CCSGG 1 cut(s) 674
NcoI CCATGG 2 cut(s) 1849, 2022
NdeI CATATG 2 cut(s) 2077, 4360
NmeAIII GCCGAG 2 cut(s) 3535, 5443
NruI TCGCGA 1 cut(s) 3043
NsiI ATGCAT 1 cut(s) 1377
NspI RCATGY 7 cut(s) 405, 1563, 1593, 3764, 4418, 4637, 5174
NspV TTCGAA 1 cut(s) 3260
OliI CACNNNNGTG 2 cut(s) 2262, 5660
PacI TTAATTAA 1 cut(s) 4890
PaeI GCATGC 1 cut(s) 4418
PaeR7I CTCGAG 2 cut(s) 60, 4990
PagI TCATGA 3 cut(s) 466, 1227, 1261
PceI AGGCCT 2 cut(s) 1344, 4689
PciI ACATGT 2 cut(s) 4633, 5170
PciSI GCTCTTC 1 cut(s) 3603
PctI GAATGC 1 cut(s) 4171
PdmI GAANNNNTTC 4 cut(s) 1215, 2547, 2643, 4672
PflFI GACNNNGTC 1 cut(s) 4856
PleI GAGTC 6 cut(s) 1239, 2916, 3046, 3189, 5486, 5636
PmaCI CACGTG 2 cut(s) 5037, 5630
PmlI CACGTG 2 cut(s) 5037, 5630
PpsI GAGTC 6 cut(s) 1239, 2916, 3046, 3189, 5486, 5636
Ppu21I YACGTR 3 cut(s) 5037, 5100, 5630
PpuMI RGGWCCY 2 cut(s) 2967, 3058
PscI ACATGT 2 cut(s) 4633, 5170
PshAI GACNNNNGTC 1 cut(s) 1540
PshBI ATTAAT 2 cut(s) 1940, 3186
PsiI TTATAA 1 cut(s) 6245
Psp124BI GAGCTC 2 cut(s) 4778, 5679
Psp5II RGGWCCY 2 cut(s) 2967, 3058
PspCI CACGTG 2 cut(s) 5037, 5630
PspEI GGTNACC 2 cut(s) 1165, 3125
PspFI CCCAGC 2 cut(s) 1693, 4721
PspPI GGNCC 8 cut(s) 319, 608, 2018, 2967, 3058, 3719, 4331, 4373
PspPPI RGGWCCY 2 cut(s) 2967, 3058
PspXI VCTCGAGB 2 cut(s) 60, 4990
PsrI GAACNNNNNNTAC 2 cut(s) 174, 206
PstI CTGCAG 6 cut(s) 706, 2866, 3372, 4381, 4711, 5996
PstNI CAGNNNCTG 7 cut(s) 2183, 2750, 3443, 3566, 3977, 4208, 4850
PsuI RGATCY 8 cut(s) 1988, 2851, 4294, 4507, 4847, 5456, 5878, 6086
PsyI GACNNNGTC 1 cut(s) 4856
PvuII CAGCTG 2 cut(s) 2459, 4205
RruI TCGCGA 1 cut(s) 3043
SacI GAGCTC 2 cut(s) 4778, 5679
SalI GTCGAC 1 cut(s) 4545
SapI GCTCTTC 1 cut(s) 3603
Sau96I GGNCC 8 cut(s) 319, 608, 2018, 2967, 3058, 3719, 4331, 4373
ScaI AGTACT 4 cut(s) 1744, 4099, 4315, 4895
SchI GAGTC 6 cut(s) 1240, 2917, 3047, 3189, 5486, 5636
SduI GDGCHC 3 cut(s) 4778, 5328, 5679
SfcI CTRYAG 9 cut(s) 702, 2141, 2862, 3368, 4377, 4707, 4954, 5992, 6394
Sfr274I CTCGAG 2 cut(s) 60, 4990
SfuI TTCGAA 1 cut(s) 3260
SgrAI CRCCGGYG 1 cut(s) 488
SinI GGWCC 4 cut(s) 2018, 2967, 3058, 3719
SlaI CTCGAG 2 cut(s) 60, 4990
SmlI CTYRAG 9 cut(s) 60, 275, 314, 2522, 2845, 4051, 4990, 5708, 5735
SmoI CTYRAG 9 cut(s) 60, 275, 314, 2522, 2845, 4051, 4990, 5708, 5735
SpeI ACTAGT 3 cut(s) 4640, 5636, 6403
SphI GCATGC 1 cut(s) 4418
SseBI AGGCCT 2 cut(s) 1344, 4689
SspI AATATT 3 cut(s) 2242, 5471, 5506
SstI GAGCTC 2 cut(s) 4778, 5679
StuI AGGCCT 2 cut(s) 1344, 4689
StyI CCWWGG 5 cut(s) 535, 1849, 2022, 2448, 4001
TatI WGTACW 9 cut(s) 1742, 2826, 3908, 4097, 4134, 4199, 4313, 4893, 6442
TspGWI ACGGA 3 cut(s) 426, 2876, 3736
Tth111I GACNNNGTC 1 cut(s) 4856
Vha464I CTTAAG 2 cut(s) 4051, 5735
VneI GTGCAC 1 cut(s) 5324
VpaK11BI GGWCC 4 cut(s) 2018, 2967, 3058, 3719
VspI ATTAAT 2 cut(s) 1940, 3186
XagI CCTNNNNNAGG 1 cut(s) 584
XbaI TCTAGA 3 cut(s) 4021, 4277, 4831
XceI RCATGY 7 cut(s) 405, 1563, 1593, 3764, 4418, 4637, 5174
XcmI CCANNNNNNNNNTGG 2 cut(s) 1856, 4875
XhoI CTCGAG 2 cut(s) 60, 4990
XmaJI CCTAGG 1 cut(s) 4001
XmiI GTMKAC 3 cut(s) 3750, 4546, 5821
XmnI GAANNNNTTC 4 cut(s) 1215, 2547, 2643, 4672
ZraI GACGTC 1 cut(s) 1192
ZrmI AGTACT 4 cut(s) 1744, 4099, 4315, 4895
Zsp2I ATGCAT 1 cut(s) 1377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.