pycom04g11750

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0007182
Physical Location & Seq
Reverse (-)
14586539 .. 14594127
7589 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g11750.3

Sequence Viewer

Length: 4818 bp
ATGGAAGCCGATTTGCCTCCTGAATACACGCACATCAAGCATCGTGATCGAATCGTGCGGAGGCTAGCTCAACTGGGAGTGCCAAACGAGTTTCTTGGTGAATACCAACGTGGGTTAGTTGCTTTTATTAAGAATAACAAGCAACGGATACCGGAACTGGTTTCCGCTATACTACCCGCTGATGACGAAGTAGCTCTAAGTGAAGCAAAACCAGGGACTAAGAAACAAGGTGTAAGCATGAAAAGTCAATTCCACGAAAGCATGTCGTGGTTGCAGTGGTTGATGTTTGAGGGTGAACCTAAGTCAGCCCTGAAGAGTCTCTCCAAAATGAGTGTTGGGCAGCGTGGTATTTGTGGAGCTGTTTGGGGACATAGCGATATAGCTTACAGATGCCGAACATGTGCAAATGATCCAACATGTGCAATTTGTGTTCCGTGCTTTCAGAATGGGAACCACAAGGATCATGATTATTCAGTTATTTATACTGGTGGTGGTTGTTGTGATTGTGGTGATGTTACAGCATGGAAGCGCAAAGGCTTTTGCTCAAATCATAAAGGTGCGGAACAGATACAACCTCTTCCAAAGGAGATTGCAAACATTGTAGGGCCTGTTCTTGATTGCCTCTTTGATATATGGAAAACCAAGCTGGTTCTTGGTGAAACTATCTGGCGGGAAAATGCTAGAGCAAGTGACCAAGTTGGAGAACGTAGAAAGGTTGAAAATGAGTTAACTTTTTTCGTGGTTGAGATGCTACTAGAATTCTGTAAGTACAGTGAGAGTTTGCTAAGTTTTGTATCCAAGACGGTTTTAGCATCAGGGGATCTATTAGGGATTCTTATGAGGGCAGAAAGGTTTTTGAATGACACTGTTACGAAAAGGCTGCATGAGTTGCTACTGAAACTGCTGGGAGAACCTGTTTTCAAATATGAGTTTGCCAAAGTATTTTTGAGTTATTACCCAGCTGTTGTGGGTGAAGCCAGAGAGGAATTAAGCCACCCTTCTGTAAAAAAGTATCCACTGCTCTTTGCATTCTCTGTTCAAATCTTCACGGTGCCAACTTTAACACCTCGTCTTGTGAAAGAAATGAACCTGCTGTCTATGCTGATGGGATGCTTGGAAGATATTTTCATTTCCTGTGCTGGAGATGACGGTCGTTTACAGGTTACCAAGTTTTCAAATTCGTATGAGATCACAATTCGTGTGATTGAAGATATTAGATTTGTTATGAGCCATGCTACAGTGCCCAAATATGTAACTCATGACCAGCAAAATATTTCAAGAACTTGGATGAGACTCTTGGCTTTTGTGCAAGGCATGAACCCTCAAAAAAGGGAAACAGGCCTGCACATTGAAGAAGAAAGTGAGAATATGCATATGCCGTTTGTACTAGGTCATTCAATTGCCAACATTCATTCTTTATTGGTGGATGGGGCATTTTCTGTAGCTATTGACAAGATGGATGAAGACTCTCTTTTCAGAACATATAAGCAGGATACGGATGACAGGGATAGCCTAAGACATTCAAAAGTAGGACGGCTGTCCCAGGAAAGTTCTGCATGTAGTGCAGTGGGGAGTAGTTCATTTGCGTGTGAGTCCAAGGTGTCTGAAGATAAATCAGATGCTCTTTCTGATTTGTTAATTCCACCATCTGTGATGTGGTTAACATATGAGTGCTTGAGGGCAATTGAGAAGTGGCTGGGAGGTGATAATACCTCCAGGACTCTTCTTGATGCATCTTCTCCAAGTACTTGCAATTTCTCTGCTAGCAATTTTTCAGCATTGAAGAGAACATTATCCAAGATCAGGAAAGGAAAATATATTTTTGGTAGACTTGCCAGTTCTAGTGAAGATCATGGCAAGCAGTGTTCATCACAATTACGTAGTGATTGCAATATGAGTGTTGACGCCCAGAATGGCAGAATTGCAGGGCAGGAGAATAATTTGATGGTTACTGACAACATTGATTCAGTCAATGCCTGCAATCCTGCAGGATTTGATGATAGTGCTATGGAAGTAGATGGTGCCACGGACTTAGATGCTCTACGTGTATTGAGTTTGTCTGATTGGCCAGACATAACATATGATGTTAGTTCACAGGATATATCTGTTCACATTCCTTTACATCGCTTACTTTCCTTGCTTTTACAGAAAGCATTAAGAAGATGTTTTGGTGAAGTGCCAGATCTGGCTAGCGTTACTTCTGCAAATTCATCATCGGCTATTTTAACTGACTTCTTTGGAAATATTCTGGGAGGCTGCCACCCGTATGGATTTTCTGCCTTTGTTATGGAGCATCCCCTTCGGATCAGAGTATTTTGTGCTGAGGTTCATGCGGGAATGTGGCGGAAAAATGGGGATGCAGCCCTATTATCTTGTGAGTGGTATCGTTCAGTTCGCTGGTCTGAACAGGGTTTGGAGCTTGATCTGTTTCTACTTCAATGCTGTGCTGCCTTGGCTCCAGCTGATCCTTATGTTAACAGAATTGTAGAACGCTTTGGCCTGTCCAGTTACCTTTCTTTGAACCTTGAGCACTCTAGTGAATATGAGACTGTTCTAGTGCAAGAAATGCTCACTCTTATTATACAAATAGTCAAAGAAAGGCGATTTTGTGGTCTAACTAAAGCTGAAAGTTTGAAAAGAGAGTTGGTTCATAAATTAGCCATTGCGGACAGCACTCATAGTCAATTGGTTAAATCTCTTCCTCGTGATCTGTCCAAGTCTGATCAGCTACATGGAATTTTGGAATCCGTTGCCGTGTACTCCAATCCCTCTGGCTTTAATCAGGGGACTTATTCGCTACAATGGACATATTGGAAAGAACTGGATTTGTACTACCCTCGTTGGAACTCGAGGGATCTGCAAGCTGCTGAAGAAAGATACTTGCGTTTTCATGGTGTTTCTGCATTGACCACTCAGCTACCCAGATGGACTAAGATTTATTCTCCTTTCAAAGGGGTAGCTAGAATTGCTACATGTAAAGCAGTCCTTAAAATTATCCGTGCTGTGCTGTTTTATGCTGTTTCTTCTGATAAATCAAGTGAGTCACGGGCTCCTGATGGTGTTCTGTTAACTGCATTGCACTTACTTTCGTTAGCATTAGACATTTGTTCTCAACATAAGGAAGCTGGTGATAAGTCTTGTTATGATGGAGATGCAATTCCCATGCTAGCTTTTGCTGGGAAAGAAATCAATGAAGGACGATACTTTGGTGCTGGTCAACAAAGCTTGCTGTCACTTCTTGTTATCTTGATGAGGATGTATAAGAAAGAGAATGTCAACAATTGTTTGGAAGCTGGAAGTTGTGACCTCTCTTCTTTAATTGGAAGTTTATTAAAGAAGTTTGTAGAGATTGATTCCGGATGTTTGACCATATTACAACTTGCACCTGAAGTGGTTGGTCTTGTGTCGCAATCTTCTCCAAATGTTGACGCTAAGACCTCAGGTTCAATTTCTGACTCTGAGAAGCGCAAGGCAAAAGCCAGAGAGAGACAGGCTGCCATATTGGAAAAAATGAGAGCCGAACAATCCAGGTTTATGGCCAGTGTTAATTCCACTGTGGACGAGGGTTCAAAATCGGAGCAAGAGGTTTGTGAACTTAATGTTGAAGATGATTTGGAAGAGTCTGAACAAGTTGTTTGCTCTCTTTGTCATGATCCCAGTTCCAGAAATCCGATATCCTACTTGGTTTTACTTCAGAAATCTAGACTTTTGAGTTTCATAGAAAGAGGTCCCTTGTCATGGGAGCAACCACGTTGGATAGACAAGGAGCACATCTCTATTACCACAAAAGAGGTGAATGATCAATCCGGATTAAGCACTTTATCAAGTGGTTCAGGACCACAACTGGTGCCATCTGATCCTTGTAGACATTTGGTTCAGAATGCAGTCACCGAATTTGCCTGTCATGGACAACCTAGGGATGTTGACGCGCTTATAGATTTTCTCAAGGGCTGGTTCCATGAATTAAGGAATATCCAAGTGCAACGTGAATCAAATGATGAAAGGGAAAATACTTTATATACATTTGAGACAATGGAAGATGATATGTACATCTCCATCCAAAAGGAAATGCGTGATAAGATGCTTCATTCTAATCCTTTAGAGGATAAGGGATTTTCAACTCCTGAAGGGGATCAACAAGAAACTAAGCATGCTGAATTTATGTTACTTGGAAAATATATAGCTTCTCTTTCTAGAGAGACAACAGAGAATCCTTCATCTTCCGAAAGTGCTCCTAATGAAAGGGTGACAGTAGATTCTTCTTGTCTTTCAGCACATGACGGATTTGGCCCGACAGATTGTGATGGAATTTATCTTTCTTCCTGTGGACATGCTGTACATCAGGGATGCCTCGATCGTTATTTATCTTCGTTGAAGGAAAGGTATCTCAGAAGAATTGTTTTTGAAGGGGGACATATTGCAGATCCCGACAAGGGGGAGTTTCTCTGCCCTGTATGCCGTCGACTTGCAAATTCTGTCTTGCCCGCATTGCCAGAGTTTCTCCAGGTCACTAAGGAGCCTCTGTATTCAGGTGTTAGTTCATCGCATGCTACTGGTCAATCAGTCAAATCAGGTGACGAAATTAATTCTCTTCAGCTTCAACAAGGGTTGGCTCTCTTGCAATCTGCAGCAGAGGCATCTGGGAAGGTTGGAAGTCTAAAAGGTTTCCCCCTTCAGAGATGTGGAAGAATGACCTCAGACCTTGAACCAATTTTTCGATTGCTCTGTAAGATGTATTTTCTGTCTAAACAGGATAAGTTATCAGGATCGATCCCAGGAAGGTGTCAACCACCCAATGCTTATGTGGGACACAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000151 GO:0000280 GO:0001101 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006259 GO:0006310 GO:0006325 GO:0006342 GO:0006355 GO:0006464 GO:0006508 GO:0006511 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0007049 GO:0007127 GO:0007131 GO:0007140 GO:0007141 GO:0007276 GO:0007283 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009605 GO:0009607 GO:0009620 GO:0009719 GO:0009725 GO:0009737 GO:0009889 GO:0009890 GO:0009892 GO:0009894 GO:0009966 GO:0009968 GO:0009987 GO:0010029 GO:0010033 GO:0010243 GO:0010468 GO:0010498 GO:0010528 GO:0010529 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010646 GO:0010648 GO:0016043 GO:0016458 GO:0016567 GO:0016569 GO:0016570 GO:0016574 GO:0016597 GO:0016740 GO:0019216 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0019941 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0023051 GO:0023057 GO:0030163 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031406 GO:0032006 GO:0032007 GO:0032446 GO:0032501 GO:0032504 GO:0032991 GO:0033522 GO:0033993 GO:0034641 GO:0035825 GO:0036094 GO:0036211 GO:0040029 GO:0042221 GO:0043161 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043177 GO:0043200 GO:0043201 GO:0043207 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0044703 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048232 GO:0048285 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0048609 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0050994 GO:0051171 GO:0051172 GO:0051239 GO:0051252 GO:0051253 GO:0051276 GO:0051321 GO:0051603 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0061630 GO:0061659 GO:0061982 GO:0065007 GO:0070647 GO:0070728 GO:0070887 GO:0071229 GO:0071230 GO:0071233 GO:0071310 GO:0071417 GO:0071495 GO:0071596 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097305 GO:0098542 GO:0140013 GO:0140096 GO:1900140 GO:1901360 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902494 GO:1902531 GO:1902532 GO:1902679 GO:1903046 GO:1903506 GO:1903507 GO:1990234 GO:2000026 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-UBR

Protein Analysis

1606

Amino Acids

178.92

Weight (kDa)

5.84

Isoelectric Point (pI)

47.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1098
AccB1I GGYRCC 3 cut(s) 1051, 2021, 3847
AccI GTMKAC 3 cut(s) 1828, 3865, 4462
AccII CGCG 1 cut(s) 3929
AccIII TCCGGA 2 cut(s) 3356, 3806
AciI CCGC 9 cut(s) 58, 165, 177, 560, 670, 2333, 2344, 2666, 4485
AcoI YGGCCR 2 cut(s) 2066, 3537
AcsI RAATTY 8 cut(s) 758, 1177, 2206, 2736, 3893, 4157, 4308, 4471
AcuI CTGAAG 8 cut(s) 332, 1626, 2889, 3408, 3677, 4146, 4577, 4658
AcyI GRCGYC 1 cut(s) 1905
AfaI GTAC 7 cut(s) 770, 1386, 1747, 2759, 2831, 4049, 4338
AfiI CCNNNNNNNGG 5 cut(s) 1803, 2951, 3738, 4433, 4434
AflIII ACRYGT 4 cut(s) 398, 416, 2044, 2972
AjnI CCWGG 6 cut(s) 211, 1542, 1715, 3527, 4503, 4774
AjuI GAANNNNNNNTTGG 2 cut(s) 2627, 2659
AleI CACNNNNGTG 1 cut(s) 2535
Alw21I GWGCWC 3 cut(s) 2532, 3771, 4234
Alw26I GTCTC 6 cut(s) 323, 1285, 2540, 3481, 4022, 4193
Ama87I CYCGRG 1 cut(s) 2848
Aor13HI TCCGGA 2 cut(s) 3356, 3806
AoxI GGCC 6 cut(s) 605, 1339, 2066, 2497, 3537, 4288
ApeKI GCWGC 8 cut(s) 340, 880, 2256, 2360, 2447, 2864, 3494, 4628
ApoI RAATTY 8 cut(s) 758, 1177, 2206, 2736, 3893, 4157, 4308, 4471
AseI ATTAAT 1 cut(s) 4584
AspA2I CCTAGG 1 cut(s) 3914
AspLEI GCGC 3 cut(s) 531, 3468, 3931
AspS9I GGNCC 4 cut(s) 605, 3728, 3836, 4289
AsuNHI GCTAGC 4 cut(s) 64, 1763, 2189, 3166
AvaI CYCGRG 1 cut(s) 2848
AvaII GGWCC 2 cut(s) 3728, 3836
AvrII CCTAGG 1 cut(s) 3914
AxyI CCTNAGG 1 cut(s) 3439
BaeGI GKGCMC 1 cut(s) 1245
BaeI ACNNNNGTAYC 4 cut(s) 2869, 2902, 3193, 3226
BalI TGGCCA 2 cut(s) 2068, 3539
BanI GGYRCC 3 cut(s) 1051, 2021, 3847
BanII GRGCYC 1 cut(s) 3052
BauI CACGAG 1 cut(s) 2703
BbsI GAAGAC 1 cut(s) 1470
Bbv12I GWGCWC 3 cut(s) 2532, 3771, 4234
BbvCI CCTCAGC 1 cut(s) 2322
BbvI GCAGC 8 cut(s) 352, 867, 2243, 2372, 2434, 2851, 3481, 4640
BceAI ACGGC 4 cut(s) 1363, 1550, 2738, 4443
BcgI CGANNNNNNTGC 8 cut(s) 29, 63, 862, 896, 2282, 2316, 2839, 2873
BciT130I CCWGG 6 cut(s) 213, 1544, 1717, 3529, 4505, 4776
BciVI GTATCC 4 cut(s) 141, 805, 1023, 1486
BclI TGATCA 2 cut(s) 2722, 3799
BcoDI GTCTC 6 cut(s) 323, 1285, 2540, 3481, 4022, 4193
BfmI CTRYAG 4 cut(s) 1236, 1440, 1986, 4626
BfuAI ACCTGC 1 cut(s) 1098
BfuI GTATCC 4 cut(s) 141, 805, 1023, 1486
BglII AGATCT 1 cut(s) 2182
BisI GCNGC 8 cut(s) 341, 881, 2257, 2361, 2448, 2865, 3495, 4629
BlnI CCTAGG 1 cut(s) 3914
BlsI GCNGC 8 cut(s) 342, 882, 2258, 2362, 2449, 2866, 3496, 4630
BmcAI AGTACT 1 cut(s) 1747
Bme1390I CCNGG 6 cut(s) 213, 1544, 1717, 3529, 4505, 4776
Bme18I GGWCC 2 cut(s) 3728, 3836
BmeT110I CYCGRG 1 cut(s) 2848
BmgT120I GGNCC 4 cut(s) 605, 3728, 3836, 4289
BmiI GGNNCC 9 cut(s) 452, 1053, 2023, 2457, 3051, 3730, 3849, 3956, 4518
BmrFI CCNGG 6 cut(s) 213, 1544, 1717, 3529, 4505, 4776
BmrI ACTGGG 2 cut(s) 83, 3651
BmtI GCTAGC 4 cut(s) 68, 1767, 2193, 3170
BmuI ACTGGG 2 cut(s) 83, 3651
BoxI GACNNNNGTC 1 cut(s) 1537
BpiI GAAGAC 1 cut(s) 1470
BplI GAGNNNNNCTC 4 cut(s) 52, 84, 3758, 3790
BpmI CTGGAG 4 cut(s) 1161, 1699, 2442, 4487
Bpu10I CCTNAGC 1 cut(s) 2322
BpuEI CTTGAG 3 cut(s) 1696, 2546, 3929
Bsa29I ATCGAT 1 cut(s) 4769
BsaAI YACGTR 2 cut(s) 1880, 2045
BsaHI GRCGYC 1 cut(s) 1905
BsaJI CCNNGG 7 cut(s) 212, 1542, 1596, 2025, 2451, 3914, 4774
BsaWI WCCGGW 3 cut(s) 151, 3356, 3806
BsaXI ACNNNNNCTCC 4 cut(s) 900, 930, 4508, 4538
Bsc4I CCNNNNNNNGG 5 cut(s) 1803, 2951, 3738, 4433, 4434
Bse21I CCTNAGG 1 cut(s) 3439
Bse3DI GCAATG 3 cut(s) 2661, 3074, 4487
BseAI TCCGGA 2 cut(s) 3356, 3806
BseBI CCWGG 6 cut(s) 213, 1544, 1717, 3529, 4505, 4776
BseCI ATCGAT 1 cut(s) 4769
BseDI CCNNGG 7 cut(s) 212, 1542, 1596, 2025, 2451, 3914, 4774
BseLI CCNNNNNNNGG 5 cut(s) 1803, 2951, 3738, 4433, 4434
BseMI GCAATG 3 cut(s) 2661, 3074, 4487
BseMII CTCAG 6 cut(s) 2313, 2927, 3450, 3453, 4402, 4710
BseSI GKGCMC 1 cut(s) 1245
BseXI GCAGC 8 cut(s) 352, 867, 2243, 2372, 2434, 2851, 3481, 4640
BseYI CCCAGC 4 cut(s) 904, 958, 1696, 3176
BsgI GTGCAG 2 cut(s) 1328, 1584
Bsh1236I CGCG 1 cut(s) 3929
Bsh1285I CGRYCG 2 cut(s) 1153, 4357
BshFI GGCC 6 cut(s) 607, 1341, 2068, 2499, 3539, 4290
BshNI GGYRCC 3 cut(s) 1051, 2021, 3847
BshVI ATCGAT 1 cut(s) 4769
BsiEI CGRYCG 2 cut(s) 1153, 4357
BsiHKAI GWGCWC 3 cut(s) 2532, 3771, 4234
BsiHKCI CYCGRG 1 cut(s) 2848
BsiSI CCGG 3 cut(s) 152, 3357, 3807
BslFI GGGAC 6 cut(s) 229, 381, 1525, 2800, 3714, 4425
BslI CCNNNNNNNGG 5 cut(s) 1803, 2951, 3738, 4433, 4434
BsmAI GTCTC 6 cut(s) 323, 1285, 2540, 3481, 4022, 4193
BsmFI GGGAC 6 cut(s) 229, 381, 1525, 2800, 3714, 4425
BsmI GAATGC 2 cut(s) 1028, 3886
BsnI GGCC 6 cut(s) 607, 1341, 2068, 2499, 3539, 4290
BsoBI CYCGRG 1 cut(s) 2848
Bsp1286I GDGCHC 5 cut(s) 1245, 2532, 3052, 3771, 4234
Bsp13I TCCGGA 2 cut(s) 3356, 3806
Bsp1407I TGTACA 2 cut(s) 4047, 4336
BspACI CCGC 9 cut(s) 58, 165, 177, 560, 670, 2333, 2344, 2666, 4485
BspANI GGCC 6 cut(s) 607, 1341, 2068, 2499, 3539, 4290
BspCNI CTCAG 6 cut(s) 2314, 2926, 3451, 3452, 4401, 4709
BspDI ATCGAT 1 cut(s) 4769
BspEI TCCGGA 2 cut(s) 3356, 3806
BspFNI CGCG 1 cut(s) 3929
BspHI TCATGA 3 cut(s) 463, 1258, 3649
BspLI GGNNCC 9 cut(s) 452, 1053, 2023, 2457, 3051, 3730, 3849, 3956, 4518
BspMAI CTGCAG 2 cut(s) 1990, 4630
BspMI ACCTGC 1 cut(s) 1098
BspOI GCTAGC 4 cut(s) 68, 1767, 2193, 3170
BspT107I GGYRCC 3 cut(s) 1051, 2021, 3847
BsrDI GCAATG 3 cut(s) 2661, 3074, 4487
BsrGI TGTACA 2 cut(s) 4047, 4336
BssECI CCNNGG 7 cut(s) 212, 1542, 1596, 2025, 2451, 3914, 4774
BssNI GRCGYC 1 cut(s) 1905
BssSI CACGAG 1 cut(s) 2703
BssT1I CCWWGG 3 cut(s) 1596, 2451, 3914
Bst2BI CACGAG 1 cut(s) 2703
Bst2UI CCWGG 6 cut(s) 213, 1544, 1717, 3529, 4505, 4776
Bst4CI ACNGT 9 cut(s) 773, 805, 868, 1051, 1151, 1240, 2551, 3556, 4252
Bst6I CTCTTC 8 cut(s) 308, 582, 1728, 1778, 2703, 3316, 3612, 4596
BstACI GRCGYC 1 cut(s) 1905
BstAPI GCANNNNNTGC 3 cut(s) 889, 1562, 2566
BstAUI TGTACA 2 cut(s) 4047, 4336
BstBAI YACGTR 2 cut(s) 1880, 2045
BstDSI CCRYGG 1 cut(s) 2025
BstEII GGTNACC 1 cut(s) 1162
BstENI CCTNNNNNAGG 1 cut(s) 2949
BstFNI CGCG 1 cut(s) 3929
BstHHI GCGC 3 cut(s) 531, 3468, 3931
BstMAI GTCTC 6 cut(s) 323, 1285, 2540, 3481, 4022, 4193
BstMCI CGRYCG 2 cut(s) 1153, 4357
BstNI CCWGG 6 cut(s) 213, 1544, 1717, 3529, 4505, 4776
BstNSI RCATGY 8 cut(s) 265, 402, 420, 1560, 2976, 4154, 4334, 4550
BstPAI GACNNNNGTC 1 cut(s) 1537
BstPI GGTNACC 1 cut(s) 1162
BstSCI CCNGG 6 cut(s) 211, 1542, 1715, 3527, 4503, 4774
BstSFI CTRYAG 4 cut(s) 1236, 1440, 1986, 4626
BstSLI GKGCMC 1 cut(s) 1245
BstSNI TACGTA 1 cut(s) 1880
BstUI CGCG 1 cut(s) 3929
BstV1I GCAGC 8 cut(s) 352, 867, 2243, 2372, 2434, 2851, 3481, 4640
BstV2I GAAGAC 1 cut(s) 1470
BstX2I RGATCY 4 cut(s) 820, 2182, 2854, 4423
BstXI CCANNNNNNTGG 2 cut(s) 2267, 3535
BstYI RGATCY 4 cut(s) 820, 2182, 2854, 4423
Bsu15I ATCGAT 1 cut(s) 4769
Bsu36I CCTNAGG 1 cut(s) 3439
BsuI GTATCC 4 cut(s) 141, 805, 1023, 1486
BsuRI GGCC 6 cut(s) 607, 1341, 2068, 2499, 3539, 4290
BsuTUI ATCGAT 1 cut(s) 4769
BtgI CCRYGG 1 cut(s) 2025
BtgZI GCGATG 2 cut(s) 2108, 4527
BtsI GCAGTG 4 cut(s) 281, 1016, 1572, 1868
BtsIMutI CAGTG 9 cut(s) 281, 778, 864, 1016, 1245, 1572, 1868, 3547, 3552
BveI ACCTGC 1 cut(s) 1098
CciI TCATGA 3 cut(s) 463, 1258, 3649
CfoI GCGC 3 cut(s) 531, 3468, 3931
Cfr13I GGNCC 4 cut(s) 605, 3728, 3836, 4289
ClaI ATCGAT 1 cut(s) 4769
CseI GACGC 3 cut(s) 1913, 3437, 3935
Csp6I GTAC 7 cut(s) 769, 1385, 1746, 2758, 2830, 4048, 4337
CviQI GTAC 7 cut(s) 769, 1385, 1746, 2758, 2830, 4048, 4337
EaeI YGGCCR 2 cut(s) 2066, 3537
Eam1104I CTCTTC 8 cut(s) 308, 582, 1728, 1778, 2703, 3316, 3612, 4596
EarI CTCTTC 8 cut(s) 308, 582, 1728, 1778, 2703, 3316, 3612, 4596
EciI GGCGGA 1 cut(s) 2359
Eco105I TACGTA 1 cut(s) 1880
Eco130I CCWWGG 3 cut(s) 1596, 2451, 3914
Eco147I AGGCCT 1 cut(s) 1341
Eco24I GRGCYC 1 cut(s) 3052
Eco32I GATATC 1 cut(s) 3675
Eco47I GGWCC 2 cut(s) 3728, 3836
Eco57I CTGAAG 8 cut(s) 332, 1626, 2889, 3408, 3677, 4146, 4577, 4658
Eco81I CCTNAGG 1 cut(s) 3439
Eco88I CYCGRG 1 cut(s) 2848
Eco91I GGTNACC 1 cut(s) 1162
EcoNI CCTNNNNNAGG 1 cut(s) 2949
EcoO109I RGGNCCY 2 cut(s) 605, 3728
EcoO65I GGTNACC 1 cut(s) 1162
EcoRI GAATTC 1 cut(s) 758
EcoRII CCWGG 6 cut(s) 211, 1542, 1715, 3527, 4503, 4774
EcoRV GATATC 1 cut(s) 3675
EcoT14I CCWWGG 3 cut(s) 1596, 2451, 3914
EcoT22I ATGCAT 2 cut(s) 1374, 1735
EcoT38I GRGCYC 1 cut(s) 3052
ErhI CCWWGG 3 cut(s) 1596, 2451, 3914
FalI AAGNNNNNCTT 6 cut(s) 982, 1014, 1455, 1487, 2970, 3002
FaqI GGGAC 6 cut(s) 229, 381, 1525, 2800, 3714, 4425
FauI CCCGC 4 cut(s) 184, 663, 2326, 4492
FauNDI CATATG 3 cut(s) 1374, 1666, 2080
FbaI TGATCA 2 cut(s) 2722, 3799
FblI GTMKAC 3 cut(s) 1828, 3865, 4462
Fnu4HI GCNGC 8 cut(s) 341, 881, 2257, 2361, 2448, 2865, 3495, 4629
FriOI GRGCYC 1 cut(s) 3052
Fsp4HI GCNGC 8 cut(s) 341, 881, 2257, 2361, 2448, 2865, 3495, 4629
GlaI GCGC 3 cut(s) 530, 3467, 3930
GluI GCNGC 8 cut(s) 341, 881, 2257, 2361, 2448, 2865, 3495, 4629
GsaI CCCAGC 4 cut(s) 908, 962, 1700, 3180
GsuI CTGGAG 4 cut(s) 1161, 1699, 2442, 4487
HaeIII GGCC 6 cut(s) 607, 1341, 2068, 2499, 3539, 4290
HapII CCGG 3 cut(s) 152, 3357, 3807
HgaI GACGC 3 cut(s) 1913, 3437, 3935
HhaI GCGC 3 cut(s) 531, 3468, 3931
Hin1I GRCGYC 1 cut(s) 1905
Hin6I GCGC 3 cut(s) 529, 3466, 3929
HinP1I GCGC 3 cut(s) 529, 3466, 3929
HindIII AAGCTT 1 cut(s) 3223
HpaI GTTAAC 4 cut(s) 729, 1662, 2476, 3069
HpaII CCGG 3 cut(s) 152, 3357, 3807
Hpy99I CGWCG 1 cut(s) 4464
HpyCH4III ACNGT 9 cut(s) 773, 805, 868, 1051, 1151, 1240, 2551, 3556, 4252
HpyCH4IV ACGT 6 cut(s) 109, 706, 1879, 2044, 3751, 3985
HpySE526I ACGT 6 cut(s) 109, 706, 1879, 2044, 3751, 3985
Hsp92I GRCGYC 1 cut(s) 1905
HspAI GCGC 3 cut(s) 529, 3466, 3929
Kpn2I TCCGGA 2 cut(s) 3356, 3806
Ksp22I TGATCA 2 cut(s) 2722, 3799
KspAI GTTAAC 4 cut(s) 729, 1662, 2476, 3069
Lsp1109I GCAGC 8 cut(s) 352, 867, 2243, 2372, 2434, 2851, 3481, 4640
MaeII ACGT 6 cut(s) 109, 706, 1879, 2044, 3751, 3985
MfeI CAATTG 4 cut(s) 1398, 1683, 2684, 3280
MflI RGATCY 4 cut(s) 820, 2182, 2854, 4423
MhlI GDGCHC 5 cut(s) 1245, 2532, 3052, 3771, 4234
MlsI TGGCCA 2 cut(s) 2068, 3539
MluNI TGGCCA 2 cut(s) 2068, 3539
MlyI GAGTC 8 cut(s) 325, 1287, 1460, 1601, 1714, 3050, 3449, 3629
MmeI TCCRAC 5 cut(s) 437, 679, 2822, 3734, 4630
Mox20I TGGCCA 2 cut(s) 2068, 3539
Mph1103I ATGCAT 2 cut(s) 1374, 1735
MroI TCCGGA 2 cut(s) 3356, 3806
MscI TGGCCA 2 cut(s) 2068, 3539
MslI CAYNNNNRTG 5 cut(s) 555, 1585, 1669, 2265, 2535
Msp20I TGGCCA 2 cut(s) 2068, 3539
MspA1I CMGCKG 3 cut(s) 179, 962, 2462
MspI CCGG 3 cut(s) 152, 3357, 3807
MspR9I CCNGG 6 cut(s) 213, 1544, 1717, 3529, 4505, 4776
MunI CAATTG 4 cut(s) 1398, 1683, 2684, 3280
Mva1269I GAATGC 2 cut(s) 1028, 3886
MvaI CCWGG 6 cut(s) 213, 1544, 1717, 3529, 4505, 4776
MvnI CGCG 1 cut(s) 3929
NdeI CATATG 3 cut(s) 1374, 1666, 2080
NheI GCTAGC 4 cut(s) 64, 1763, 2189, 3166
NlaIV GGNNCC 9 cut(s) 452, 1053, 2023, 2457, 3051, 3730, 3849, 3956, 4518
NmuCI GTSAC 8 cut(s) 689, 3042, 3231, 3302, 3886, 4246, 4507, 4574
NsiI ATGCAT 2 cut(s) 1374, 1735
NspI RCATGY 8 cut(s) 265, 402, 420, 1560, 2976, 4154, 4334, 4550
OliI CACNNNNGTG 1 cut(s) 2535
PaeI GCATGC 2 cut(s) 4154, 4550
PaeR7I CTCGAG 1 cut(s) 2848
PagI TCATGA 3 cut(s) 463, 1258, 3649
PceI AGGCCT 1 cut(s) 1341
PciI ACATGT 3 cut(s) 398, 416, 2972
PctI GAATGC 2 cut(s) 1028, 3886
PfeI GAWTC 8 cut(s) 51, 832, 1964, 2744, 3353, 3989, 4210, 4256
PfoI TCCNGGA 1 cut(s) 1715
PkrI GCNGC 8 cut(s) 342, 882, 2258, 2362, 2449, 2866, 3496, 4630
Ple19I CGATCG 1 cut(s) 4357
PleI GAGTC 8 cut(s) 324, 1287, 1460, 1600, 1714, 3049, 3449, 3628
PpsI GAGTC 8 cut(s) 324, 1287, 1460, 1600, 1714, 3049, 3449, 3628
Ppu21I YACGTR 2 cut(s) 1880, 2045
PpuMI RGGWCCY 1 cut(s) 3728
PscI ACATGT 3 cut(s) 398, 416, 2972
PshAI GACNNNNGTC 1 cut(s) 1537
PshBI ATTAAT 1 cut(s) 4584
Psp5II RGGWCCY 1 cut(s) 3728
Psp6I CCWGG 6 cut(s) 211, 1542, 1715, 3527, 4503, 4774
PspEI GGTNACC 1 cut(s) 1162
PspFI CCCAGC 4 cut(s) 904, 958, 1696, 3176
PspGI CCWGG 6 cut(s) 211, 1542, 1715, 3527, 4503, 4774
PspN4I GGNNCC 9 cut(s) 452, 1053, 2023, 2457, 3051, 3730, 3849, 3956, 4518
PspPI GGNCC 4 cut(s) 605, 3728, 3836, 4289
PspPPI RGGWCCY 1 cut(s) 3728
PspXI VCTCGAGB 1 cut(s) 2848
PsrI GAACNNNNNNTAC 2 cut(s) 594, 626
PstI CTGCAG 2 cut(s) 1990, 4630
PsuI RGATCY 4 cut(s) 820, 2182, 2854, 4423
PvuI CGATCG 1 cut(s) 4357
PvuII CAGCTG 2 cut(s) 962, 2462
RsaI GTAC 7 cut(s) 770, 1386, 1747, 2759, 2831, 4049, 4338
RsaNI GTAC 7 cut(s) 769, 1385, 1746, 2758, 2830, 4048, 4337
RseI CAYNNNNRTG 5 cut(s) 555, 1585, 1669, 2265, 2535
SalI GTCGAC 1 cut(s) 4461
SatI GCNGC 8 cut(s) 341, 881, 2257, 2361, 2448, 2865, 3495, 4629
Sau96I GGNCC 4 cut(s) 605, 3728, 3836, 4289
SbfI CCTGCAGG 1 cut(s) 1990
ScaI AGTACT 1 cut(s) 1747
SchI GAGTC 8 cut(s) 325, 1287, 1460, 1601, 1714, 3050, 3449, 3629
ScrFI CCNGG 6 cut(s) 213, 1544, 1717, 3529, 4505, 4776
SdaI CCTGCAGG 1 cut(s) 1990
SduI GDGCHC 5 cut(s) 1245, 2532, 3052, 3771, 4234
SfcI CTRYAG 4 cut(s) 1236, 1440, 1986, 4626
Sfr274I CTCGAG 1 cut(s) 2848
SinI GGWCC 2 cut(s) 3728, 3836
SlaI CTCGAG 1 cut(s) 2848
SmiMI CAYNNNNRTG 5 cut(s) 555, 1585, 1669, 2265, 2535
SmlI CTYRAG 4 cut(s) 1675, 2525, 2848, 3944
SmoI CTYRAG 4 cut(s) 1675, 2525, 2848, 3944
SnaBI TACGTA 1 cut(s) 1880
SphI GCATGC 2 cut(s) 4154, 4550
Sse8387I CCTGCAGG 1 cut(s) 1990
SseBI AGGCCT 1 cut(s) 1341
SsiI CCGC 9 cut(s) 58, 165, 177, 560, 670, 2333, 2344, 2666, 4485
SspI AATATT 2 cut(s) 1273, 2245
StuI AGGCCT 1 cut(s) 1341
StyD4I CCNGG 6 cut(s) 211, 1542, 1715, 3527, 4503, 4774
StyI CCWWGG 3 cut(s) 1596, 2451, 3914
TaaI ACNGT 9 cut(s) 773, 805, 868, 1051, 1151, 1240, 2551, 3556, 4252
TaiI ACGT 6 cut(s) 112, 709, 1882, 2047, 3754, 3988
TaqI TCGA 6 cut(s) 49, 2849, 4353, 4462, 4717, 4769
TatI WGTACW 7 cut(s) 768, 1384, 1745, 2757, 2829, 4047, 4336
TfiI GAWTC 8 cut(s) 51, 832, 1964, 2744, 3353, 3989, 4210, 4256
TscAI CASTG 9 cut(s) 281, 778, 871, 1023, 1245, 1572, 1868, 3547, 3559
TseFI GTSAC 8 cut(s) 689, 3042, 3231, 3302, 3886, 4246, 4507, 4574
TseI GCWGC 8 cut(s) 340, 880, 2256, 2360, 2447, 2864, 3494, 4628
Tsp45I GTSAC 8 cut(s) 689, 3042, 3231, 3302, 3886, 4246, 4507, 4574
TspGWI ACGGA 7 cut(s) 160, 423, 1511, 2042, 2737, 2987, 4296
TspRI CASTG 9 cut(s) 281, 778, 871, 1023, 1245, 1572, 1868, 3547, 3559
VpaK11BI GGWCC 2 cut(s) 3728, 3836
VspI ATTAAT 1 cut(s) 4584
XagI CCTNNNNNAGG 1 cut(s) 2949
XapI RAATTY 8 cut(s) 758, 1177, 2206, 2736, 3893, 4157, 4308, 4471
XbaI TCTAGA 2 cut(s) 3701, 4193
XceI RCATGY 8 cut(s) 265, 402, 420, 1560, 2976, 4154, 4334, 4550
XcmI CCANNNNNNNNNTGG 2 cut(s) 1653, 4801
XhoI CTCGAG 1 cut(s) 2848
XmaJI CCTAGG 1 cut(s) 3914
XmiI GTMKAC 3 cut(s) 1828, 3865, 4462
ZrmI AGTACT 1 cut(s) 1747
Zsp2I ATGCAT 2 cut(s) 1374, 1735
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.