FvH4_6g13660

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0007182
Physical Location & Seq
Forward (+)
8327456 .. 8341092
13637 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g13660.t3

Sequence Viewer

Length: 6288 bp
ATGTCTTCCATGGAAATCGATTCCCCTTCTGAATCTACCCCCCGTGATCGAATCATTCAGAGGCTTGCTCTACTGGGAGTGCCTCAGAAGCTACTTAATCTGCAGCAACATGGTTTAGTTGCTTTTGTTAAGAATAACAAGCAAATGTTGCCGGAACTGGTTTCGGCTATCTTGCCTCCTGATGAGGAAGTAGCGGAGGTTCTCCGCGAGGCAAAACCAGGGTCGAAGAAACAGTTAGCCGGTATTACTATGAAAGTTCAATTCCGTGAGAGCATGTTGTGGCTGAAGTGGTTGATGTTTGAGGGTGAACCTACTGTGGCTATGAAGAATCTGTCGAAGATGAGTGTGGGGCAGCGGGGTATTTGTGGAGCTGTTTGGGGGCATAATGATATAGCCTTCAGGTGCAAAACATGTGAGCATGATCCGACCTGTGCAATTTGCGTTCCTTGTTTTAAGAATGGGAACCACAAGGATCATGATTACTCGATTATATATACTGGTGGTGGTTGTTGTGATTGTGGTGATGTTACAGCGTGGAAACGCCAAGGGTTTTGCTCAAAGCATAAAGGTGCAGAACAGATACAGCCCCTTCCAGAGGAGTTTGCTATCATGGTGGGGCCTGTTCTTCGCTGCCTCTTTGCCTGTTGGAAGAGCAAGCTACTTCTTTCTGAAACTGCCTGCCGGGAAGGCCCTAGAGTAACTGATATTGCTGCAGAGCGTAAGAAGGTTGCAAATGAGTTGACCTATTTTGTGGTTGAGATGCTTCTGGACTTCTGCAAGTGCAGTGAGAGTTTGCTTAGTTTTGTATCGAGTATGATTTTATCATCGGTTGACTTTTTGGGGATTTTGGTGAGGGCAGAGAGGTTTTTGAGTGATGCTGTTGTGAAAAAGCTCCATGAGTTGCTCCTGAAATTGATGGGGGAGCCTGTTTTCAAATACGAGTTTGCCAAAGTATTTCTCAATTATTACCCAACTGTTGTTAGTGAAGCCATAAAGGAGTTCACTGACCTTGCTTTGAAAAAGTATCCACTGCTCTCTATATTCTCTGTACAAATCCTCACGGTGCCAACTTTAACACCGCGTCTTGTGAAAGAAATGAACCTACTATCAATGCTGTTAGGGTGTTTGGAAGATATTTTCAATTCTTGTGCTGGTGAAGATGGCCGTTTACAGGTTACCAAGTGGTCAAATTTGTATGACATCACCATTCGTGTGATTGAAGATTTTCGGTTTGTTATGAGTCATGCTATAGTGCCAAAATATGTAACTCATGAGAAGCAAGATATTTTGAGAACTTGGATGAGACTCTCAGCTTTTGTGCAAGGGATGAGCCCACAAAAGAGGGAAACAGGCCTCCATATTGAAGAAGAGAATGACACTATGCATCTGCCATTCGTTCTGGGTCATTCAATTGCCAACCTTCATTCTTTGTTGGTGGATGGAGCATTTTCTGTTGCTAGTGACCAGACGGAAGAAGATTTATTTCTCAGCACAAATAAGCAAGATATGGATGACACTGATACACTAAGACATGCAAAAGTTGGCCGGCTGTCACAGGAAAGTTCTGCATGTACTGCGGTGGGGAGTAGTTCATTGACGTTTTCTGAAGATAAATCAAATGCTCTTTCTTATTCTTTGATACCATCATCTGTGACGTGGTTAACATTTGAGTGTTTGAGGGCTATTGAGAATTGGCTGGGAGTTGATAACGCCTCCGGTACAATTCTTGATGTATCATCTTCAAGTACTAGCAATTTCTCTGGGAGCAATTTTTCAGCATTGAAGAGAACATTATCCAAATTTAGGAAAGGCAAAAACATTTTTGGTAGATTTTCCAGTTCAAGTGAAGACCATGGCAAGCATACTTCATCACATCTACATAATAGTTCTGATATGAGTGTTGACATCCAGAACGGCAAACTTTCAGGGCAGGAAAACAAATTAATGCCTATGGACGAAATTGATTTAGCTAATGCCTGTAATTCGGCAGGATTGGGTGATGGTGCAATGGAAATAGATGGTCCCATGGACTTAGATGCTCTACGTGTACTGAGTTCATCTGACTGGCCGGACATAACATATGATGTTAGTTCACAGGATATATCTGTTCACATTCCCTTACATCGCTTACTTGCGTTGCTTATACAGAGTTCATTAAGAAGGTGTTTTGGCGAAGAGCCAGATTCTGGTGCAGCTAGTTCTGCAGATTTATCATCAGCTATTTCCACAGACCTTTTTGGAACTATCCTCGGAGGCTGTCATCCATGTGGGTTTTCTGCCTTTGTTATGGAGCATCCTCTTCGTATACGAGTATTTTGTGCTCAGGTTCACGCTGGAATGTGGCGGAAAAATGGGGATGCTGCACCATTAACTTGCGAGTGGTATCGTTCAGTTCGCTGGTCGGAACAGTTTGTGGAGCTTGATCTATTTCTACTTCAGTGCTGTGCTACCTTGGCTCCAGCTGATCTTTATATCAAAAGAATTCTAGAACGCTTTGGCCTGTCAAGCTACCTTTCTCTGAAACTTGAGCGTTCTAGTGAATATGAACCAGTTCTGGTGCAGGAAATGCTCACTCTTATTATTCAAATAATCAAAGAGAGGCGATTCTGTGGGTTAACTAAAGCTGAAAGTGTGAAAAGAGAATTGATTCATAAATTAAGCATTGCAGATGCTACTCACAGTCAGTTGGTTAAGTCTCTTCCTCGTGATCTGACCAAATTTCACCAGCTTAATGAAATTTTGGATACAGTTGCTGTGTATTCCAATCCCTCTGGCTTTAACCAGGGGACTTATTCACTACAATGGACATTTTGGAAAGAACTGGATTTGTACTATCATCGTTGGAACTCAAGGGATCTACAAGCTGCAGAAGAAAGATACTTGCGTTTCCGTGGTGTATCTGCATTGACAAGTCAGCTACCCAGGTGGACTAAGATTTATCCTCCTTTCAGGGGTTTAGCTAGAATAGGGACATGTAAAACTGTCCTCCAAATTGTTCGTGCAGTGCTGTTTTATGCTGTATTTTCTGACAAGTCGACTGAGTCGCGAGCACCTGATGGGGTCCTCTTAACTGCATTGCACGTGCTCTCATTAGCATTAGACATTTGTTCTCAACATAAAGAATCTGGTGACCATTTTTGTACTGACGGAGATTTCATTCCAGTGCTTACTTTTGCCTGTGAAGAAATTAGTGAAGGACTCTATTTTGAGGCTGGTCAACAAAGCTTGTTGTCACTTCTTGTTATTTTGATGAGGATGTATTCGAAAGAGGGTTTAGATAACTCAGAAGATGGAAGTTGGAACCTTTCTTCTTTAATTGGAAACTTACTGAAGAAGTTTGTGGTTATTGATAGTGGATGTATGACCAAACTGCAAGTACTTGCACCTGAGTTGGTTAGTCATGTAACTCTTCCAAATAGTGATACAGTTATCTCAGGTTCAGCTTCTGATTCTGAGAAGCGAAAGGCAAAGGCCAAAGAGAGACAGGCTGCCATACTGGAAAAAATGAGAGCCGAGCAATCAAAGTTTTTGAGTAGCATTGATTCCTCAGTGGATGATGGTTCTGAAGCTGAACCAAAGGATGTTGACTCTGATGTCGAAGATAATTCAGAAGAGCCTGCACAAGTTGTTTGCTCGCTCTGCCATGACCCAAATTCCAAGAGTCCTATATCCTTCTTGGTTCTTCTTCAAAAGTCTAGGCTTTTGAGTTTCATTGATAGAGGTCCACTATCATGGGATCAACCTCGCTCTGTAGACAAGGAGCATGTTCCTAAAACCAAAGATGAGGTGATTGATCAATCTGGTATAAGCAGATTGTTTAGTGGTTCAGGAGTGGTTTCCTCGTCTCATGTAAGACAGTTGGTGTCAGATCACTACAACAGAAAAAAGGCGATTGGTAAATCTGGAATAAATACACTGTCAAGTGATTCAGGAGTGGTGTCACCTCATGATCTATGGCGGTTGGTTGAGAAGGCAGTCACTGAGTTTGCCCTACATGGGAGACCTAGGGATGTTGATGCTTTTCTAGAGGTTCTGAAGGGCCGGTTCCATGATTTGAGGAATATCAAAGTGCCATGTGAATTAAATGATGAAAAGGAAAGTACTTCATATGCCTTTGAGACAATGGAAGAAGATATGTATGTCTGCATCCGTAGAGAAGTGCATGATAAGCTGCATTCCAAGCTTACAGAAGATCAGAAATGTACAACTGCTGACGGGGATCGAGAGAATACAGAACATACTGAATCTTTGTTGCTTGGATACTATATAGCTGCTCTATCTAGAGAGAGAAGAGAAGATCCTTCATCTTCTGAAAGTTCTCCTAATGATAAGGGGCCAATAGAATGTTCTCGCCTGTTGGCATGTGATGGATTTGGTCCTGCAGATTGTGATGGAATTTATCTATCTTCCTGTGGACATGCTGTGCATCAGGAATGTCTTGATCGTTATTTGTCTTCCCTAAAGGAAAGGTATCTCAGGAGAATTGTCTTTGAAGGAGGACATATAGTAGATCCAGATAAGGGTGAATTTCTCTGCCCTGTATGCCGTCGTCTTGCAAATTCAGTCTTGCCTACATTACCAAGTGAGTTGCAGAAGGTTCGCAAGGAGCCTATGGATACAGGTGTTAGTTCATCACATGTTACTAGTTCATCATGCAAACCAGCCGAAGGAATTAGTTCCCTTCAGCAAGGCTTGGCTCTCTTGCAATCTGCAGCAAATGCAGGTGGGAAGGTTGGCGCACTCAAAGATTTCCCCCTGCGTAGATGTGAACAAAGGAACCCAAATCTTGAACCAATCTCAAGTCTGCTCTCTAAGATGTATTTCCCAACTGATCTGGATAAGATATCAGGATCTGACAGGGTCAGCCACCCAATGCTTATGTGGGACTTAGTTAAGTACTCCCTATTGTCAATGGAGATCGCTTCTCGTTCCGGGGGAAAGTATGCAGCACCAAGCTACAGCCTTAATGCCTTGTATAAGGAACTTGAATCCTCAAGCAGATTCATATTGTCCTTGTTGCTGAAACTTATCCAAAACACATGTAAGAATTCTCTTCATGTGCTTCAGAGATTTATAGCAACTAAGAGCTTTGCAGAATCTACGTGCTTTGGAATTTCCGTAGTTCATGGAAGCAAAACATCTGGACAAGGTGCTATGCTGCATATCTTGGAACATCTTGATAATCCAGTAGCATATCCTGATATCCAGTTCTGGAGTCGAGCTTCTGATCCTGTGCTTGCCCGTGATCCTTTTTCTTCATTGATGTGGGTTCTATTTTGTCTTCCATACCGATTTTTATCGTGTGAAGATTCCTTGCTATCACTGGTGCATGTTTTTTATGTTGTCTCTGTGGTTCAGGGTATTACTACATATTTGGGGAAAAATCAAAATGATGTTAGTGGATTAGGTGATGGTGATTGCCTGATTACTGACATCAGCAGGCTTATGGGAGAATTTGGATCTGCTCACCAGTATTTTGCATCAAATTATATAGACTCATCTTCTAATATTAAGAATATAGTTCGTAGCTTGAGTTTCCCTTATTTGCGGCGGTGTGCACTGCTGTTGAAGCTACTTGATTCCTATGCCCAAGTGCCATTCTGTGAAAGGTATAATGCATTGGATAGATCACGTGCCACTAGTGACGCAATAGATACCACTTATGTGCCACTGGTTGAGCTCAATGAAGTTCAAGAGATAGAAACACTGCTCAAGATTCCTGTTTTGGATGTTATTCTTAAGGATACAGAGGTGCGTTCTTTAGCTCATAAATGGTTCTGTCATTTTGGCAAAGAGTACGAGGACAAAAGGTTTCAAGGCACTATACACTGCAACCCTGCAGTTCCATTCCAATTGATGCGTCTTCCCCGGGTTTACCAGGATCTATTGCAGCGGTATATAAAGCAGCGGTGCCGTGATTGCAATAACATACTGGATGAACCTGCATTGTGCCTACTTTGTGGTAGGTTATGTTCTCCTAGCTGGAAGTCATGCTGCAGGGAAAGTGGATGCCAAACTCATGCAGTGGCTTGTGGTTCTGGTACTGGGATCTTTCTGTTGATCAGGAGAACAACAATACTTTTGCAAAGATCTGCACGGCAGGCTCCCTGGCCATCACCCTACTTGGATGCTTTTGGGGAAGAGGACATTGAAATGCAGAGAGGAAAACCACTGTTCTTGAACGAAGAACGTTATGCAGCTTTAACTTATTTGGTTGCTTCTCATGGCCTTGATCGAAGTTCGAAGGTTCTCGGGCAAACTACTATTGGTTCTTTCTTCATGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000151 GO:0000280 GO:0001101 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006259 GO:0006310 GO:0006325 GO:0006342 GO:0006355 GO:0006464 GO:0006508 GO:0006511 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0007049 GO:0007127 GO:0007131 GO:0007140 GO:0007141 GO:0007276 GO:0007283 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009605 GO:0009607 GO:0009620 GO:0009719 GO:0009725 GO:0009737 GO:0009889 GO:0009890 GO:0009892 GO:0009894 GO:0009966 GO:0009968 GO:0009987 GO:0010029 GO:0010033 GO:0010243 GO:0010468 GO:0010498 GO:0010528 GO:0010529 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010646 GO:0010648 GO:0016043 GO:0016458 GO:0016567 GO:0016569 GO:0016570 GO:0016574 GO:0016597 GO:0016740 GO:0019216 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0019941 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0023051 GO:0023057 GO:0030163 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031406 GO:0032006 GO:0032007 GO:0032446 GO:0032501 GO:0032504 GO:0032991 GO:0033522 GO:0033993 GO:0034641 GO:0035825 GO:0036094 GO:0036211 GO:0040029 GO:0042221 GO:0043161 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043177 GO:0043200 GO:0043201 GO:0043207 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0044703 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048232 GO:0048285 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0048609 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0050994 GO:0051171 GO:0051172 GO:0051239 GO:0051252 GO:0051253 GO:0051276 GO:0051321 GO:0051603 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0061630 GO:0061659 GO:0061982 GO:0065007 GO:0070647 GO:0070728 GO:0070887 GO:0071229 GO:0071230 GO:0071233 GO:0071310 GO:0071417 GO:0071495 GO:0071596 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097305 GO:0098542 GO:0140013 GO:0140096 GO:1900140 GO:1901360 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902494 GO:1902531 GO:1902532 GO:1902679 GO:1903046 GO:1903506 GO:1903507 GO:1990234 GO:2000026 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-UBR

Protein Analysis

2096

Amino Acids

234.06

Weight (kDa)

6.04

Isoelectric Point (pI)

50.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_UBR1 PF22960 466 - 550 1.3e-19 E3 ubiquitin-protein ligase UBR1-like, winged-helix domain
PRT6_C PF18995 1192 - 1668 5.7e-90 Proteolysis_6 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 4712
AasI GACNNNNNNGTC 1 cut(s) 3593
Acc36I ACCTGC 2 cut(s) 4712, 5950
AccB1I GGYRCC 2 cut(s) 1063, 5910
AccB7I CCANNNNNTGG 1 cut(s) 2186
AccI GTMKAC 3 cut(s) 2305, 3035, 3753
AccII CGCG 3 cut(s) 207, 1081, 3046
AclI AACGTT 1 cut(s) 6190
AcoI YGGCCR 4 cut(s) 1162, 1543, 2066, 6110
AcuI CTGAAG 9 cut(s) 305, 382, 1626, 2420, 3350, 3585, 4055, 4667, 5048
AcvI CACGTG 2 cut(s) 3082, 5633
AfiI CCNNNNNNNGG 8 cut(s) 595, 1171, 1803, 2186, 2951, 3996, 4520, 4667
AflII CTTAAG 1 cut(s) 5738
AflIII ACRYGT 5 cut(s) 410, 2044, 2972, 4636, 5039
AhlI ACTAGT 2 cut(s) 4643, 5639
AjiI CACGTC 1 cut(s) 1656
AjnI CCWGG 5 cut(s) 217, 2781, 2921, 5877, 6107
AjuI GAANNNNNNNTTGG 2 cut(s) 4330, 4362
AleI CACNNNNGTG 1 cut(s) 5663
Alw21I GWGCWC 5 cut(s) 2323, 3052, 3087, 5560, 5682
Alw26I GTCTC 7 cut(s) 1297, 2700, 3475, 3849, 3994, 4112, 5350
Alw44I GTGCAC 1 cut(s) 5556
AlwNI CAGNNNCTG 5 cut(s) 2186, 2753, 3446, 3980, 4853
Ama87I CYCGRG 2 cut(s) 5868, 6251
ApaLI GTGCAC 1 cut(s) 5556
ArsI GACNNNNNNTTYG 2 cut(s) 206, 238
AseI ATTAAT 1 cut(s) 1943
Asp700I GAANNNNTTC 4 cut(s) 1224, 2550, 2646, 4675
AspA2I CCTAGG 1 cut(s) 4004
AspLEI GCGC 1 cut(s) 4739
AspS9I GGNCC 8 cut(s) 617, 689, 2021, 3061, 3722, 4039, 4334, 4376
AsuC2I CCSGG 4 cut(s) 683, 4933, 5869, 5870
AsuII TTCGAA 2 cut(s) 3263, 6242
AvaI CYCGRG 2 cut(s) 5868, 6251
AvaII GGWCC 4 cut(s) 2021, 3061, 3722, 4376
AvrII CCTAGG 1 cut(s) 4004
BaeGI GKGCMC 1 cut(s) 5560
BaeI ACNNNNGTAYC 6 cut(s) 2869, 2902, 4608, 4641, 5736, 5769
BalI TGGCCA 1 cut(s) 6112
BanI GGYRCC 2 cut(s) 1063, 5910
BanII GRGCYC 2 cut(s) 1334, 5682
BauI CACGAG 1 cut(s) 2703
BbrPI CACGTG 2 cut(s) 3082, 5633
BbsI GAAGAC 4 cut(s) 1854, 4446, 5273, 5855
Bbv12I GWGCWC 5 cut(s) 2323, 3052, 3087, 5560, 5682
BceAI ACGGC 5 cut(s) 1149, 1930, 4530, 5898, 6113
BcgI CGANNNNNNTGC 4 cut(s) 2282, 2316, 4580, 4614
BciT130I CCWGG 5 cut(s) 219, 2783, 2923, 5879, 6109
BciVI GTATCC 5 cut(s) 1035, 2737, 4253, 4609, 5737
BclI TGATCA 2 cut(s) 3793, 6060
BcnI CCSGG 4 cut(s) 683, 4933, 5869, 5870
BcoDI GTCTC 7 cut(s) 1297, 2700, 3475, 3849, 3994, 4112, 5350
BcuI ACTAGT 2 cut(s) 4643, 5639
BfrI CTTAAG 1 cut(s) 5738
BfuAI ACCTGC 2 cut(s) 4712, 5950
BfuI GTATCC 5 cut(s) 1035, 2737, 4253, 4609, 5737
BglI GCCNNNNNGGC 1 cut(s) 687
BglII AGATCT 1 cut(s) 6089
BlnI CCTAGG 1 cut(s) 4004
BmcAI AGTACT 4 cut(s) 1747, 3378, 4102, 4898
Bme1390I CCNGG 9 cut(s) 219, 683, 2783, 2923, 4933, 5869, 5870, 5879, 6109
Bme18I GGWCC 4 cut(s) 2021, 3061, 3722, 4376
BmeT110I CYCGRG 2 cut(s) 5868, 6251
BmgBI CACGTC 1 cut(s) 1656
BmgT120I GGNCC 8 cut(s) 617, 689, 2021, 3061, 3722, 4039, 4334, 4376
BmrFI CCNGG 9 cut(s) 219, 683, 2783, 2923, 4933, 5869, 5870, 5879, 6109
BmrI ACTGGG 2 cut(s) 83, 6054
BmuI ACTGGG 2 cut(s) 83, 6054
BpiI GAAGAC 4 cut(s) 1854, 4446, 5273, 5855
BplI GAGNNNNNCTC 2 cut(s) 52, 84
BpmI CTGGAG 2 cut(s) 2442, 5233
Bpu10I CCTNAGC 1 cut(s) 2322
Bpu14I TTCGAA 2 cut(s) 3263, 6242
BpuEI CTTGAG 6 cut(s) 2546, 2833, 4783, 4978, 5551, 5696
BpuMI CCSGG 4 cut(s) 683, 4933, 5869, 5870
Bsa29I ATCGAT 1 cut(s) 18
BsaAI YACGTR 4 cut(s) 2045, 3082, 5103, 5633
BsaBI GATNNNNATC 1 cut(s) 5296
BsaI GGTCTC 1 cut(s) 3994
BsaWI WCCGGW 1 cut(s) 1715
BsaXI ACNNNNNCTCC 2 cut(s) 2440, 2470
Bsc4I CCNNNNNNNGG 8 cut(s) 595, 1171, 1803, 2186, 2951, 3996, 4520, 4667
Bse118I RCCGGY 3 cut(s) 239, 1545, 4041
Bse3DI GCAATG 3 cut(s) 2013, 2661, 3074
Bse8I GATNNNNATC 1 cut(s) 5296
BseBI CCWGG 5 cut(s) 219, 2783, 2923, 5879, 6109
BseCI ATCGAT 1 cut(s) 18
BseJI GATNNNNATC 1 cut(s) 5296
BseLI CCNNNNNNNGG 8 cut(s) 595, 1171, 1803, 2186, 2951, 3996, 4520, 4667
BseMI GCAATG 3 cut(s) 2013, 2661, 3074
BseRI GAGGAG 1 cut(s) 611
BseSI GKGCMC 1 cut(s) 5560
BseYI CCCAGC 1 cut(s) 1696
BsgI GTGCAG 8 cut(s) 591, 802, 2211, 2346, 2579, 3021, 3603, 6078
Bsh1236I CGCG 3 cut(s) 207, 1081, 3046
BshNI GGYRCC 2 cut(s) 1063, 5910
BshVI ATCGAT 1 cut(s) 18
BsiHKAI GWGCWC 5 cut(s) 2323, 3052, 3087, 5560, 5682
BsiHKCI CYCGRG 2 cut(s) 5868, 6251
BsiSI CCGG 9 cut(s) 152, 240, 682, 1546, 1716, 2069, 4042, 4932, 5869
BslFI GGGAC 4 cut(s) 2007, 2800, 2983, 4898
BslI CCNNNNNNNGG 8 cut(s) 595, 1171, 1803, 2186, 2951, 3996, 4520, 4667
BsmAI GTCTC 7 cut(s) 1297, 2700, 3475, 3849, 3994, 4112, 5350
BsmBI CGTCTC 1 cut(s) 3849
BsmFI GGGAC 4 cut(s) 2007, 2800, 2983, 4898
BsmI GAATGC 1 cut(s) 4174
Bso31I GGTCTC 1 cut(s) 3994
BsoBI CYCGRG 2 cut(s) 5868, 6251
Bsp119I TTCGAA 2 cut(s) 3263, 6242
Bsp1286I GDGCHC 6 cut(s) 1334, 2323, 3052, 3087, 5560, 5682
Bsp1407I TGTACA 2 cut(s) 1048, 4202
Bsp19I CCATGG 3 cut(s) 9, 1852, 2025
Bsp68I TCGCGA 1 cut(s) 3046
BspDI ATCGAT 1 cut(s) 18
BspFNI CGCG 3 cut(s) 207, 1081, 3046
BspHI TCATGA 3 cut(s) 475, 1270, 3946
BspMAI CTGCAG 8 cut(s) 105, 715, 2206, 2869, 4384, 4714, 5842, 5999
BspMI ACCTGC 2 cut(s) 4712, 5950
BspQI GCTCTTC 3 cut(s) 644, 2169, 3606
BspT104I TTCGAA 2 cut(s) 3263, 6242
BspT107I GGYRCC 2 cut(s) 1063, 5910
BspTI CTTAAG 1 cut(s) 5738
BspTNI GGTCTC 1 cut(s) 3994
BsrDI GCAATG 3 cut(s) 2013, 2661, 3074
BsrFI RCCGGY 3 cut(s) 239, 1545, 4041
BsrGI TGTACA 2 cut(s) 1048, 4202
BssAI RCCGGY 3 cut(s) 239, 1545, 4041
BssNAI GTATAC 1 cut(s) 2306
BssSI CACGAG 1 cut(s) 2703
BssT1I CCWWGG 6 cut(s) 9, 544, 1852, 2025, 2451, 4004
Bst1107I GTATAC 1 cut(s) 2306
Bst2BI CACGAG 1 cut(s) 2703
Bst2UI CCWGG 5 cut(s) 219, 2783, 2923, 5879, 6109
BstAFI CTTAAG 1 cut(s) 5738
BstAPI GCANNNNNTGC 4 cut(s) 148, 1574, 2566, 4718
BstAUI TGTACA 2 cut(s) 1048, 4202
BstBAI YACGTR 4 cut(s) 2045, 3082, 5103, 5633
BstBI TTCGAA 2 cut(s) 3263, 6242
BstDSI CCRYGG 4 cut(s) 9, 1852, 2025, 2890
BstEII GGTNACC 2 cut(s) 1174, 3128
BstENI CCTNNNNNAGG 1 cut(s) 593
BstFNI CGCG 3 cut(s) 207, 1081, 3046
BstHHI GCGC 1 cut(s) 4739
BstMAI GTCTC 7 cut(s) 1297, 2700, 3475, 3849, 3994, 4112, 5350
BstNI CCWGG 5 cut(s) 219, 2783, 2923, 5879, 6109
BstPI GGTNACC 2 cut(s) 1174, 3128
BstSCI CCNGG 9 cut(s) 217, 681, 2781, 2921, 4931, 5867, 5868, 5877, 6107
BstSLI GKGCMC 1 cut(s) 5560
BstUI CGCG 3 cut(s) 207, 1081, 3046
BstV2I GAAGAC 4 cut(s) 1854, 4446, 5273, 5855
BstX2I RGATCY 8 cut(s) 2854, 4297, 4510, 4850, 5459, 5881, 6048, 6089
BstXI CCANNNNNNTGG 1 cut(s) 3732
BstYI RGATCY 8 cut(s) 2854, 4297, 4510, 4850, 5459, 5881, 6048, 6089
BstZ17I GTATAC 1 cut(s) 2306
Bsu15I ATCGAT 1 cut(s) 18
BsuI GTATCC 5 cut(s) 1035, 2737, 4253, 4609, 5737
BsuTUI ATCGAT 1 cut(s) 18
BtgI CCRYGG 4 cut(s) 9, 1852, 2025, 2890
BtgZI GCGATG 1 cut(s) 2108
BtrI CACGTC 1 cut(s) 1656
BtsI GCAGTG 7 cut(s) 790, 1028, 3009, 5558, 5705, 5827, 6030
BtuMI TCGCGA 1 cut(s) 3046
BveI ACCTGC 2 cut(s) 4712, 5950
CaiI CAGNNNCTG 5 cut(s) 2186, 2753, 3446, 3980, 4853
CciI TCATGA 3 cut(s) 475, 1270, 3946
CfoI GCGC 1 cut(s) 4739
Cfr10I RCCGGY 3 cut(s) 239, 1545, 4041
Cfr13I GGNCC 8 cut(s) 617, 689, 2021, 3061, 3722, 4039, 4334, 4376
Cfr9I CCCGGG 1 cut(s) 5868
ClaI ATCGAT 1 cut(s) 18
CseI GACGC 3 cut(s) 1070, 5654, 5849
CspCI CAANNNNNGTGG 2 cut(s) 6159, 6194
DrdI GACNNNNNNGTC 1 cut(s) 3593
DseDI GACNNNNNNGTC 1 cut(s) 3593
EaeI YGGCCR 4 cut(s) 1162, 1543, 2066, 6110
EciI GGCGGA 1 cut(s) 2359
Ecl136II GAGCTC 1 cut(s) 5680
Eco130I CCWWGG 6 cut(s) 9, 544, 1852, 2025, 2451, 4004
Eco147I AGGCCT 1 cut(s) 1353
Eco24I GRGCYC 2 cut(s) 1334, 5682
Eco31I GGTCTC 1 cut(s) 3994
Eco32I GATATC 2 cut(s) 4845, 5203
Eco47I GGWCC 4 cut(s) 2021, 3061, 3722, 4376
Eco53kI GAGCTC 1 cut(s) 5680
Eco57I CTGAAG 9 cut(s) 305, 382, 1626, 2420, 3350, 3585, 4055, 4667, 5048
Eco72I CACGTG 2 cut(s) 3082, 5633
Eco88I CYCGRG 2 cut(s) 5868, 6251
Eco91I GGTNACC 2 cut(s) 1174, 3128
EcoICRI GAGCTC 1 cut(s) 5680
EcoNI CCTNNNNNAGG 1 cut(s) 593
EcoO109I RGGNCCY 3 cut(s) 617, 689, 3061
EcoO65I GGTNACC 2 cut(s) 1174, 3128
EcoRI GAATTC 2 cut(s) 2481, 5047
EcoRII CCWGG 5 cut(s) 217, 2781, 2921, 5877, 6107
EcoRV GATATC 2 cut(s) 4845, 5203
EcoT14I CCWWGG 6 cut(s) 9, 544, 1852, 2025, 2451, 4004
EcoT22I ATGCAT 2 cut(s) 1386, 5620
EcoT38I GRGCYC 2 cut(s) 1334, 5682
ErhI CCWWGG 6 cut(s) 9, 544, 1852, 2025, 2451, 4004
Esp3I CGTCTC 1 cut(s) 3849
FaqI GGGAC 4 cut(s) 2007, 2800, 2983, 4898
FauI CCCGC 1 cut(s) 348
FauNDI CATATG 2 cut(s) 2080, 4108
FbaI TGATCA 2 cut(s) 3793, 6060
FblI GTMKAC 3 cut(s) 2305, 3035, 3753
FriOI GRGCYC 2 cut(s) 1334, 5682
GlaI GCGC 1 cut(s) 4738
GsaI CCCAGC 1 cut(s) 1700
GsuI CTGGAG 2 cut(s) 2442, 5233
HapII CCGG 9 cut(s) 152, 240, 682, 1546, 1716, 2069, 4042, 4932, 5869
HgaI GACGC 3 cut(s) 1070, 5654, 5849
HhaI GCGC 1 cut(s) 4739
Hin6I GCGC 1 cut(s) 4737
HinP1I GCGC 1 cut(s) 4737
HincII GTYRAC 8 cut(s) 741, 832, 1662, 1903, 2616, 3036, 3218, 3586
HindII GTYRAC 8 cut(s) 741, 832, 1662, 1903, 2616, 3036, 3218, 3586
HindIII AAGCTT 2 cut(s) 3223, 4181
HpaI GTTAAC 2 cut(s) 1662, 2616
HpaII CCGG 9 cut(s) 152, 240, 682, 1546, 1716, 2069, 4042, 4932, 5869
Hpy99I CGWCG 1 cut(s) 4551
HpyCH4IV ACGT 7 cut(s) 1598, 1655, 2044, 3081, 5102, 5632, 6190
HpySE526I ACGT 7 cut(s) 1598, 1655, 2044, 3081, 5102, 5632, 6190
HspAI GCGC 1 cut(s) 4737
KroI GCCGGC 1 cut(s) 1545
KroNI GCCGGC 1 cut(s) 1547
Ksp22I TGATCA 2 cut(s) 3793, 6060
KspAI GTTAAC 2 cut(s) 1662, 2616
LguI GCTCTTC 3 cut(s) 644, 2169, 3606
MaeII ACGT 7 cut(s) 1598, 1655, 2044, 3081, 5102, 5632, 6190
MfeI CAATTG 2 cut(s) 1410, 5852
MflI RGATCY 8 cut(s) 2854, 4297, 4510, 4850, 5459, 5881, 6048, 6089
MhlI GDGCHC 6 cut(s) 1334, 2323, 3052, 3087, 5560, 5682
MlsI TGGCCA 1 cut(s) 6112
MluNI TGGCCA 1 cut(s) 6112
MlyI GAGTC 8 cut(s) 1249, 1299, 3050, 3192, 3581, 3670, 5224, 5489
MmeI TCCRAC 5 cut(s) 449, 626, 2382, 2822, 3278
Mox20I TGGCCA 1 cut(s) 6112
Mph1103I ATGCAT 2 cut(s) 1386, 5620
MroNI GCCGGC 1 cut(s) 1545
MroXI GAANNNNTTC 4 cut(s) 1224, 2550, 2646, 4675
MscI TGGCCA 1 cut(s) 6112
Msp20I TGGCCA 1 cut(s) 6112
MspA1I CMGCKG 4 cut(s) 355, 2462, 5893, 5908
MspCI CTTAAG 1 cut(s) 5738
MspI CCGG 9 cut(s) 152, 240, 682, 1546, 1716, 2069, 4042, 4932, 5869
MspR9I CCNGG 9 cut(s) 219, 683, 2783, 2923, 4933, 5869, 5870, 5879, 6109
MunI CAATTG 2 cut(s) 1410, 5852
Mva1269I GAATGC 1 cut(s) 4174
MvaI CCWGG 5 cut(s) 219, 2783, 2923, 5879, 6109
MvnI CGCG 3 cut(s) 207, 1081, 3046
NaeI GCCGGC 1 cut(s) 1547
NciI CCSGG 4 cut(s) 683, 4933, 5869, 5870
NcoI CCATGG 3 cut(s) 9, 1852, 2025
NdeI CATATG 2 cut(s) 2080, 4108
NgoMIV GCCGGC 1 cut(s) 1545
NmeAIII GCCGAG 1 cut(s) 3538
NmuCI GTSAC 8 cut(s) 1460, 1551, 1651, 3128, 3231, 3939, 3976, 5642
NruI TCGCGA 1 cut(s) 3046
NsiI ATGCAT 2 cut(s) 1386, 5620
NspV TTCGAA 2 cut(s) 3263, 6242
OliI CACNNNNGTG 1 cut(s) 5663
PagI TCATGA 3 cut(s) 475, 1270, 3946
PaqCI CACCTGC 1 cut(s) 4712
PceI AGGCCT 1 cut(s) 1353
PciI ACATGT 4 cut(s) 410, 2972, 4636, 5039
PciSI GCTCTTC 3 cut(s) 644, 2169, 3606
PctI GAATGC 1 cut(s) 4174
PdiI GCCGGC 1 cut(s) 1547
PdmI GAANNNNTTC 4 cut(s) 1224, 2550, 2646, 4675
PflFI GACNNNGTC 2 cut(s) 3040, 4859
PflMI CCANNNNNTGG 1 cut(s) 2186
PleI GAGTC 8 cut(s) 1248, 1299, 3049, 3192, 3581, 3669, 5223, 5489
PmaCI CACGTG 2 cut(s) 3082, 5633
PmlI CACGTG 2 cut(s) 3082, 5633
PpsI GAGTC 8 cut(s) 1248, 1299, 3049, 3192, 3581, 3669, 5223, 5489
Ppu21I YACGTR 4 cut(s) 2045, 3082, 5103, 5633
PpuMI RGGWCCY 1 cut(s) 3061
PscI ACATGT 4 cut(s) 410, 2972, 4636, 5039
PshBI ATTAAT 1 cut(s) 1943
Psp124BI GAGCTC 1 cut(s) 5682
Psp1406I AACGTT 1 cut(s) 6190
Psp5II RGGWCCY 1 cut(s) 3061
Psp6I CCWGG 5 cut(s) 217, 2781, 2921, 5877, 6107
PspCI CACGTG 2 cut(s) 3082, 5633
PspEI GGTNACC 2 cut(s) 1174, 3128
PspFI CCCAGC 1 cut(s) 1696
PspGI CCWGG 5 cut(s) 217, 2781, 2921, 5877, 6107
PspPI GGNCC 8 cut(s) 617, 689, 2021, 3061, 3722, 4039, 4334, 4376
PspPPI RGGWCCY 1 cut(s) 3061
PsrI GAACNNNNNNTAC 4 cut(s) 183, 215, 5956, 5988
PstI CTGCAG 8 cut(s) 105, 715, 2206, 2869, 4384, 4714, 5842, 5999
PstNI CAGNNNCTG 5 cut(s) 2186, 2753, 3446, 3980, 4853
PsuI RGATCY 8 cut(s) 2854, 4297, 4510, 4850, 5459, 5881, 6048, 6089
PsyI GACNNNGTC 2 cut(s) 3040, 4859
PvuII CAGCTG 1 cut(s) 2462
RruI TCGCGA 1 cut(s) 3046
SacI GAGCTC 1 cut(s) 5682
SalI GTCGAC 1 cut(s) 3034
SapI GCTCTTC 3 cut(s) 644, 2169, 3606
Sau96I GGNCC 8 cut(s) 617, 689, 2021, 3061, 3722, 4039, 4334, 4376
ScaI AGTACT 4 cut(s) 1747, 3378, 4102, 4898
SchI GAGTC 8 cut(s) 1249, 1299, 3050, 3192, 3581, 3670, 5224, 5489
ScrFI CCNGG 9 cut(s) 219, 683, 2783, 2923, 4933, 5869, 5870, 5879, 6109
SduI GDGCHC 6 cut(s) 1334, 2323, 3052, 3087, 5560, 5682
SfuI TTCGAA 2 cut(s) 3263, 6242
SinI GGWCC 4 cut(s) 2021, 3061, 3722, 4376
SmaI CCCGGG 1 cut(s) 5870
SmlI CTYRAG 7 cut(s) 2525, 2848, 4798, 4993, 5530, 5711, 5738
SmoI CTYRAG 7 cut(s) 2525, 2848, 4798, 4993, 5530, 5711, 5738
SpeI ACTAGT 2 cut(s) 4643, 5639
SseBI AGGCCT 1 cut(s) 1353
SspI AATATT 1 cut(s) 5509
SstI GAGCTC 1 cut(s) 5682
StuI AGGCCT 1 cut(s) 1353
StyD4I CCNGG 9 cut(s) 217, 681, 2781, 2921, 4931, 5867, 5868, 5877, 6107
StyI CCWWGG 6 cut(s) 9, 544, 1852, 2025, 2451, 4004
TaiI ACGT 7 cut(s) 1601, 1658, 2047, 3084, 5105, 5635, 6193
TauI GCSGC 1 cut(s) 5551
TseFI GTSAC 8 cut(s) 1460, 1551, 1651, 3128, 3231, 3939, 3976, 5642
Tsp45I GTSAC 8 cut(s) 1460, 1551, 1651, 3128, 3231, 3939, 3976, 5642
TspGWI ACGGA 6 cut(s) 254, 1484, 2879, 3162, 4139, 5107
TspMI CCCGGG 1 cut(s) 5868
Tth111I GACNNNGTC 2 cut(s) 3040, 4859
Van91I CCANNNNNTGG 1 cut(s) 2186
Vha464I CTTAAG 1 cut(s) 5738
VneI GTGCAC 1 cut(s) 5556
VpaK11BI GGWCC 4 cut(s) 2021, 3061, 3722, 4376
VspI ATTAAT 1 cut(s) 1943
XagI CCTNNNNNAGG 1 cut(s) 593
XbaI TCTAGA 3 cut(s) 2485, 4024, 4280
XcmI CCANNNNNNNNNTGG 1 cut(s) 4878
XmaI CCCGGG 1 cut(s) 5868
XmaJI CCTAGG 1 cut(s) 4004
XmiI GTMKAC 3 cut(s) 2305, 3035, 3753
XmnI GAANNNNTTC 4 cut(s) 1224, 2550, 2646, 4675
ZrmI AGTACT 4 cut(s) 1747, 3378, 4102, 4898
Zsp2I ATGCAT 2 cut(s) 1386, 5620
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.