AT5G04270

Belongs to the DHHC palmitoyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0011310
Physical Location & Seq
Reverse (-)
1182626 .. 1184863
2238 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G04270.1

Sequence Viewer

Length: 816 bp
ATGAGTTGTCGGAGATTCTTCTCGATCCCGGTCCTTTTGGTGATTCTGGTGATGGGTTTCGTGTATTACGTCACACTGTTTGTTTTCATCGATGATTGGGTTGGTTTACAAAGCTCAGCTGGGAAATTAAACGCCTTACTCTTCAGCTTATTGGCGTCTCTCTGTCTCTTCTCGCTCTCAATTTGCGTTCTTGTAGATCCAGGTCGTGTTCCTGCATCTTATGCTCCTGATGTTGAGGACTCTGGCTGGTCCAATAGTAACGTTACAGAAACAAGGAAATGTGATAAGTGCTTTGCATATAAGCCTCTTCGGACTCACCATTGCCGGGTTTGCAGAAGATGCGTCTTGAAGATGGACCATCATTGCCTCTGGATAAATAACTGTGTTGGTTATGCAAACTATAAGGCTTTCTTCATTCTTGTGTTTTATGCAACCGTGGCTTCCATCTACTCTACAGTGTTGCTGGTTTGCTGTGCATTCAAGAACGGAGATTCCTATGCGGGAAATGTTCCGCTCAAAACTTTCATTGTTTCTTGTGGGATCTTCATGATCGGACTAAGCATAACACTTGGGACTCTCCTTTGTTGGCATATCTACCTCATCACACACAATATGACCACCATTGAGCATTATGATTCAAAGCGGGCCTCATGGTTAGCTAGAAAATCAGGACAAAGCTATAGGCATCAATTCGATGTTGGCTTTTACAAAAACCTCACCTCGGTGTTAGGCCCTAACATGATCAAATGGCTATGCCCCACATTTACTAGGAATCCTGAAGACGGTATTAGCTTTTCTGCATCGAGAGACAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DHHC

Protein Analysis

271

Amino Acids

30.62

Weight (kDa)

8.86

Isoelectric Point (pI)

49.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DHHC PF01529 89 - 212 2.3e-35 DHHC palmitoyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 514
AciI CCGC 3 cut(s) 500, 512, 643
AclI AACGTT 1 cut(s) 261
AclWI GGATC 3 cut(s) 19, 191, 548
AcuI CTGAAG 2 cut(s) 127, 798
AcyI GRCGYC 1 cut(s) 155
AfiI CCNNNNNNNGG 3 cut(s) 325, 721, 782
AgsI TTSAA 3 cut(s) 349, 481, 639
AjnI CCWGG 1 cut(s) 199
AleI CACNNNNGTG 1 cut(s) 722
AloI GAACNNNNNNTCC 2 cut(s) 476, 508
AluBI AGCT 7 cut(s) 114, 119, 147, 659, 678, 792, 813
AluI AGCT 7 cut(s) 114, 119, 147, 659, 678, 792, 813
Alw26I GTCTC 3 cut(s) 162, 170, 801
AlwI GGATC 3 cut(s) 19, 191, 548
AoxI GGCC 2 cut(s) 645, 730
AspS9I GGNCC 5 cut(s) 31, 249, 355, 645, 731
AsuC2I CCSGG 2 cut(s) 29, 326
AsuHPI GGTGA 4 cut(s) 52, 61, 308, 709
AvaII GGWCC 3 cut(s) 31, 249, 355
BbsI GAAGAC 1 cut(s) 786
BccI CCATC 4 cut(s) 46, 346, 366, 452
BciT130I CCWGG 1 cut(s) 201
BclI TGATCA 1 cut(s) 741
BcnI CCSGG 2 cut(s) 29, 326
BcoDI GTCTC 3 cut(s) 162, 170, 801
BfaI CTAG 3 cut(s) 660, 768, 814
BfmI CTRYAG 2 cut(s) 453, 679
BlpI GCTNAGC 1 cut(s) 115
Bme1390I CCNGG 3 cut(s) 29, 201, 326
Bme18I GGWCC 3 cut(s) 31, 249, 355
BmgT120I GGNCC 5 cut(s) 31, 249, 355, 645, 731
BmrFI CCNGG 3 cut(s) 29, 201, 326
BmsI GCATC 4 cut(s) 224, 329, 694, 809
BpiI GAAGAC 1 cut(s) 786
Bpu1102I GCTNAGC 1 cut(s) 115
BpuMI CCSGG 2 cut(s) 29, 326
Bsa29I ATCGAT 1 cut(s) 90
BsaHI GRCGYC 1 cut(s) 155
BsaJI CCNNGG 2 cut(s) 435, 720
Bsc4I CCNNNNNNNGG 3 cut(s) 325, 721, 782
Bse3DI GCAATG 2 cut(s) 319, 361
BseBI CCWGG 1 cut(s) 201
BseCI ATCGAT 1 cut(s) 90
BseDI CCNNGG 2 cut(s) 435, 720
BseLI CCNNNNNNNGG 3 cut(s) 325, 721, 782
BseMI GCAATG 2 cut(s) 319, 361
BseMII CTCAG 1 cut(s) 129
BseYI CCCAGC 1 cut(s) 119
BshFI GGCC 2 cut(s) 647, 732
BshVI ATCGAT 1 cut(s) 90
BsiSI CCGG 2 cut(s) 29, 325
BslFI GGGAC 1 cut(s) 586
BslI CCNNNNNNNGG 3 cut(s) 325, 721, 782
BsmAI GTCTC 3 cut(s) 162, 170, 801
BsmBI CGTCTC 1 cut(s) 162
BsmFI GGGAC 1 cut(s) 586
BsmI GAATGC 1 cut(s) 476
BsnI GGCC 2 cut(s) 647, 732
Bsp143I GATC 5 cut(s) 24, 196, 540, 549, 741
Bsp1720I GCTNAGC 1 cut(s) 115
BspACI CCGC 3 cut(s) 500, 512, 643
BspANI GGCC 2 cut(s) 647, 732
BspCNI CTCAG 1 cut(s) 128
BspDI ATCGAT 1 cut(s) 90
BspHI TCATGA 1 cut(s) 546
BspPI GGATC 3 cut(s) 19, 191, 548
BsrBI CCGCTC 1 cut(s) 514
BsrDI GCAATG 2 cut(s) 319, 361
BssECI CCNNGG 2 cut(s) 435, 720
BssMI GATC 5 cut(s) 24, 196, 540, 549, 741
BssNI GRCGYC 1 cut(s) 155
Bst2UI CCWGG 1 cut(s) 201
Bst4CI ACNGT 5 cut(s) 78, 383, 436, 457, 785
Bst6I CTCTTC 3 cut(s) 146, 173, 312
BstACI GRCGYC 1 cut(s) 155
BstAPI GCANNNNNTGC 2 cut(s) 221, 339
BstC8I GCNNGC 1 cut(s) 645
BstDEI CTNAG 2 cut(s) 115, 557
BstDSI CCRYGG 1 cut(s) 435
BstKTI GATC 5 cut(s) 27, 199, 543, 552, 744
BstMAI GTCTC 3 cut(s) 162, 170, 801
BstMBI GATC 5 cut(s) 24, 196, 540, 549, 741
BstMWI GCNNNNNNNGC 4 cut(s) 221, 330, 339, 437
BstNI CCWGG 1 cut(s) 201
BstSCI CCNGG 3 cut(s) 27, 199, 324
BstSFI CTRYAG 2 cut(s) 453, 679
BstV2I GAAGAC 1 cut(s) 786
BstX2I RGATCY 2 cut(s) 196, 540
BstYI RGATCY 2 cut(s) 196, 540
Bsu15I ATCGAT 1 cut(s) 90
BsuRI GGCC 2 cut(s) 647, 732
BsuTUI ATCGAT 1 cut(s) 90
BtgI CCRYGG 1 cut(s) 435
BtsIMutI CAGTG 2 cut(s) 74, 462
Cac8I GCNNGC 1 cut(s) 645
CciI TCATGA 1 cut(s) 546
Cfr13I GGNCC 5 cut(s) 31, 249, 355, 645, 731
ClaI ATCGAT 1 cut(s) 90
CseI GACGC 2 cut(s) 144, 331
CviAII CATG 3 cut(s) 547, 651, 739
DdeI CTNAG 2 cut(s) 115, 557
DpnI GATC 5 cut(s) 26, 198, 542, 551, 743
DpnII GATC 5 cut(s) 24, 196, 540, 549, 741
Eam1104I CTCTTC 3 cut(s) 146, 173, 312
EarI CTCTTC 3 cut(s) 146, 173, 312
Eco47I GGWCC 3 cut(s) 31, 249, 355
Eco57I CTGAAG 2 cut(s) 127, 798
EcoO109I RGGNCCY 1 cut(s) 731
EcoRII CCWGG 1 cut(s) 199
Esp3I CGTCTC 1 cut(s) 162
FaeI CATG 3 cut(s) 550, 654, 742
FalI AAGNNNNNCTT 2 cut(s) 395, 427
FaqI GGGAC 1 cut(s) 586
FatI CATG 3 cut(s) 546, 650, 738
FauI CCCGC 2 cut(s) 493, 636
FbaI TGATCA 1 cut(s) 741
FspBI CTAG 3 cut(s) 660, 768, 814
GsaI CCCAGC 1 cut(s) 123
HaeIII GGCC 2 cut(s) 647, 732
HapII CCGG 2 cut(s) 29, 325
HgaI GACGC 2 cut(s) 144, 331
Hin1I GRCGYC 1 cut(s) 155
Hin1II CATG 3 cut(s) 550, 654, 742
HinfI GANTC 8 cut(s) 15, 43, 239, 313, 491, 574, 635, 772
HpaII CCGG 2 cut(s) 29, 325
HphI GGTGA 4 cut(s) 52, 61, 308, 709
Hpy166II GTNNAC 1 cut(s) 107
Hpy188I TCNGA 3 cut(s) 12, 312, 554
Hpy188III TCNNGA 9 cut(s) 22, 227, 346, 370, 481, 547, 669, 776, 804
Hpy8I GTNNAC 1 cut(s) 107
HpyCH4III ACNGT 5 cut(s) 78, 383, 436, 457, 785
HpyCH4IV ACGT 2 cut(s) 69, 261
HpyCH4V TGCA 7 cut(s) 215, 296, 333, 395, 431, 476, 800
HpyF10VI GCNNNNNNNGC 4 cut(s) 221, 330, 339, 437
HpyF3I CTNAG 2 cut(s) 115, 557
HpySE526I ACGT 2 cut(s) 69, 261
Hsp92I GRCGYC 1 cut(s) 155
Hsp92II CATG 3 cut(s) 550, 654, 742
Ksp22I TGATCA 1 cut(s) 741
Kzo9I GATC 5 cut(s) 24, 196, 540, 549, 741
LmnI GCTCC 1 cut(s) 229
LweI GCATC 4 cut(s) 224, 329, 694, 809
MaeI CTAG 3 cut(s) 660, 768, 814
MaeII ACGT 2 cut(s) 69, 261
MaeIII GTNAC 3 cut(s) 70, 257, 262
MalI GATC 5 cut(s) 26, 198, 542, 551, 743
MbiI CCGCTC 1 cut(s) 514
MboI GATC 5 cut(s) 24, 196, 540, 549, 741
MboII GAAGA 9 cut(s) 10, 133, 160, 299, 348, 361, 403, 535, 791
MflI RGATCY 2 cut(s) 196, 540
MluCI AATT 3 cut(s) 125, 180, 689
MlyI GAGTC 3 cut(s) 233, 307, 568
MnlI CCTC 7 cut(s) 229, 315, 377, 608, 658, 725, 730
MseI TTAA 1 cut(s) 128
MslI CAYNNNNRTG 2 cut(s) 419, 722
MspA1I CMGCKG 1 cut(s) 119
MspI CCGG 2 cut(s) 29, 325
MspR9I CCNGG 3 cut(s) 29, 201, 326
Mva1269I GAATGC 1 cut(s) 476
MvaI CCWGG 1 cut(s) 201
MwoI GCNNNNNNNGC 4 cut(s) 221, 330, 339, 437
NciI CCSGG 2 cut(s) 29, 326
NdeII GATC 5 cut(s) 24, 196, 540, 549, 741
NlaIII CATG 3 cut(s) 550, 654, 742
NmuCI GTSAC 1 cut(s) 70
OliI CACNNNNGTG 1 cut(s) 722
PagI TCATGA 1 cut(s) 546
PcsI WCGNNNNNNNCGW 1 cut(s) 66
PctI GAATGC 1 cut(s) 476
PfeI GAWTC 5 cut(s) 15, 43, 491, 635, 772
PleI GAGTC 3 cut(s) 233, 307, 568
PpsI GAGTC 3 cut(s) 233, 307, 568
Psp1406I AACGTT 1 cut(s) 261
Psp6I CCWGG 1 cut(s) 199
PspFI CCCAGC 1 cut(s) 119
PspGI CCWGG 1 cut(s) 199
PspPI GGNCC 5 cut(s) 31, 249, 355, 645, 731
PsuI RGATCY 2 cut(s) 196, 540
PvuII CAGCTG 1 cut(s) 119
RseI CAYNNNNRTG 2 cut(s) 419, 722
SaqAI TTAA 1 cut(s) 128
Sau3AI GATC 5 cut(s) 24, 196, 540, 549, 741
Sau96I GGNCC 5 cut(s) 31, 249, 355, 645, 731
SchI GAGTC 3 cut(s) 233, 307, 568
ScrFI CCNGG 3 cut(s) 29, 201, 326
SfaNI GCATC 4 cut(s) 224, 329, 694, 809
SfcI CTRYAG 2 cut(s) 453, 679
SinI GGWCC 3 cut(s) 31, 249, 355
SmiMI CAYNNNNRTG 2 cut(s) 419, 722
Sse9I AATT 3 cut(s) 125, 180, 689
SsiI CCGC 3 cut(s) 500, 512, 643
SspMI CTAG 3 cut(s) 660, 768, 814
StyD4I CCNGG 3 cut(s) 27, 199, 324
TaaI ACNGT 5 cut(s) 78, 383, 436, 457, 785
TaiI ACGT 2 cut(s) 72, 264
TaqI TCGA 4 cut(s) 23, 90, 693, 803
TasI AATT 3 cut(s) 125, 180, 689
TfiI GAWTC 5 cut(s) 15, 43, 491, 635, 772
Tru1I TTAA 1 cut(s) 128
Tru9I TTAA 1 cut(s) 128
TscAI CASTG 2 cut(s) 81, 462
TseFI GTSAC 1 cut(s) 70
Tsp45I GTSAC 1 cut(s) 70
TspDTI ATGAA 4 cut(s) 76, 403, 514, 535
TspGWI ACGGA 1 cut(s) 501
TspRI CASTG 2 cut(s) 81, 462
VpaK11BI GGWCC 3 cut(s) 31, 249, 355
XspI CTAG 3 cut(s) 660, 768, 814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.