AT5G04420

Kelch motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005156
Physical Location & Seq
Reverse (-)
1246604 .. 1249943
3340 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G04420.1

Sequence Viewer

Length: 1545 bp
ATGGAAGAGATCAGGAAAGAAATAGATGTCGGTGATTGGCATTCAAATCTAGCTCACGATGAATGGACACCACTCCCTGTTTCTGGCTCTCGAGCATCAGCTCGTTACAAGCATGCGGCAGTGGTTGTTGATGAGAAGCTCTACATTGTTGGTGGAAGCCGGAATGGCCGATACTTATCTGATGTCCAGGTGTTTGATCTTAGAAGTTTGACTTGGTCTAGTTTGAAACTGAAAACTGAATCATCAAGTGCTGATAACATTCAAGAGGATGATGGTAGCAGCTTACGGGAAGCTTTTCCTGCTATTTCAGACCATCGCATGATTAAGTGGGGAAACAAGCTTCTTTTGATCGGGGGACACTCCAAGAAGTCATCAGATAACATGTTAGTGCGGTTCATTGATTTGGAAACACATTCATGTGGAGTCATTGATGTGTTTGGGAATGTTCCGGCTTCACGTGGTGGACATTCCATTACACTGGTTGGATCCCGAGTTCTTGTTTTTGGTGGGGAAGATAAGAACAGGAGACTGCTGAATGATCTGCATGTTCTTCATCTTGAGACAATGACTTGGGATGTTGTAGAGACAAAGCAGACACGCCCAGTTCCCAGATTTGATCACACAGCTGCCACACATTCTGATCGCTACCTCTTGATTTTTGGTGGTTGTTCTCATTCGATTTTCTACAGTGATCTTCATATCCTTGACTTGCAAACAATGGAGTGGTCACAGCCTCATGTTCAGGGAGATGTTGTAACTCCTCGGGCAGGACACGCTGGAATCACCATTGACGAAAACTGGTATATAGTTGGGGGCGGTGATAACAGTACTGGTTGTTTAGAGACCCTTGTTCTAAATATGTCAAAGCTGGTTTGGTCTACATCAACCCATGTAGAAGCAAGACATCCACTTGCTAGTGAGGGTCTCAGTGTATGTTCAGCATCAGTATTTGGAGAGAACATCTTAGTAGCTTTTGGCGGCTATAACGGGAAGTATAACAATGATATATTTGTCATGAGGCTAAAACCAGGAGAATCCTCACATCCCAAAATCTTTAAGTCACCAGCAGCCGCTGCAGCTGCAGCCTCTGTCACTGCTGCTTATGCCATAGCCAAGTCAGATAAGTCAGATTATCCTCCACCTGCAAATCCAACTCTCAATGGAAATGGAAACAGTCTTCCTGAGAGAGATATTAGGAACAGAATCGATACCATTAAAGAAGAAAAAAGAGCATTGGAGTCATCTATTGCGGAAACTCAAGTAGAAAACGCGAAGCTCAGGGAAAAGATAGATGAAGTCAATAGTAGCCACACTGAGCTATCTCAAGAACTCCAATCTGTAGAAGGTCAACTTATTTCAGAACGGTCTAGATGCTTTAAACTCGAGGCACAAATAGCAGAATTGCAGAAAGCACTGGAGTCAGGGCAATCCATCGAAGCTGAAGTTGAGATGCTTAGAAGACAGAGGTCAGCGTCTGATGAAGAAGAAGACGGGACTGTGCAAAGGCAAGGCTCTGCTGGTGTGTGGGGTTTGTTTGGAAGGTAA

Protein Analysis

514

Amino Acids

56.66

Weight (kDa)

5.65

Isoelectric Point (pI)

41.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_KLHDC2_KLHL20_DRC7 PF24681 16 - 82 7.4e-14 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 16 - 82 2e-09 FBX42, beta-propeller domain
Beta-prop_ATRN-LZTR1 PF24981 16 - 225 9.8e-26 Attractin/LZTR1 beta-propeller
Kelch_1 PF01344 34 - 75 5.7e-12 Kelch motif
Kelch_2 PF07646 35 - 76 5.5e-08 Kelch motif
Beta-prop_FBX42 PF13415 101 - 225 1.9e-25 FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 105 - 312 2.9e-52 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 115 - 272 1e-21 Attractin/LZTR1 beta-propeller
Kelch_HCF PF13854 148 - 272 5.1e-19 Host cell factor, Kelch-repeats domain
Beta-prop_TYW4 PF13418 150 - 225 4.4e-07 tRNA wybutosine-synthesizing protein 4, beta-propeller
Beta-prop_FBX42 PF13415 204 - 363 7.5e-22 FBX42, beta-propeller domain
Beta-prop_ATRN-LZTR1 PF24981 231 - 351 9.1e-08 Attractin/LZTR1 beta-propeller
ACBP4_C PF24922 400 - 499 3.2e-36 Acyl-CoA-binding domain-containing protein 4-6, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1150
Acc36I ACCTGC 1 cut(s) 1150
AccI GTMKAC 1 cut(s) 878
AccII CGCG 1 cut(s) 1271
AciI CCGC 6 cut(s) 116, 391, 816, 978, 1071, 1250
AclWI GGATC 2 cut(s) 480, 493
AcoI YGGCCR 1 cut(s) 166
AcuI CTGAAG 1 cut(s) 1461
AcvI CACGTG 1 cut(s) 458
AdeI CACNNNGTG 1 cut(s) 461
AfaI GTAC 1 cut(s) 829
AfiI CCNNNNNNNGG 2 cut(s) 83, 767
AflIII ACRYGT 1 cut(s) 381
AgsI TTSAA 3 cut(s) 45, 226, 263
AjnI CCWGG 2 cut(s) 186, 1027
AjuI GAANNNNNNNTTGG 2 cut(s) 196, 228
AloI GAACNNNNNNTCC 2 cut(s) 477, 509
Alw26I GTCTC 5 cut(s) 520, 554, 578, 836, 929
AlwI GGATC 2 cut(s) 480, 493
AlwNI CAGNNNCTG 3 cut(s) 1073, 1088, 1475
Ama87I CYCGRG 4 cut(s) 90, 489, 762, 1382
AoxI GGCC 1 cut(s) 166
ApeKI GCWGC 8 cut(s) 279, 626, 1067, 1073, 1076, 1079, 1082, 1097
Asp700I GAANNNNTTC 1 cut(s) 294
AsuHPI GGTGA 4 cut(s) 44, 775, 830, 1053
AvaI CYCGRG 4 cut(s) 90, 489, 762, 1382
BamHI GGATCC 1 cut(s) 485
BbrPI CACGTG 1 cut(s) 458
BbsI GAAGAC 3 cut(s) 1169, 1465, 1494
BbvI GCAGC 8 cut(s) 291, 613, 1060, 1066, 1079, 1084, 1088, 1094
BccI CCATC 3 cut(s) 266, 321, 1439
BciT130I CCWGG 2 cut(s) 188, 1029
BclI TGATCA 1 cut(s) 616
BcoDI GTCTC 5 cut(s) 520, 554, 578, 836, 929
BfaI CTAG 4 cut(s) 50, 219, 915, 1368
BfmI CTRYAG 4 cut(s) 685, 1074, 1080, 1338
BfuAI ACCTGC 1 cut(s) 1150
BglI GCCNNNNNGGC 1 cut(s) 165
BmcAI AGTACT 1 cut(s) 829
Bme1390I CCNGG 2 cut(s) 188, 1029
BmeT110I CYCGRG 4 cut(s) 90, 489, 762, 1382
BmiI GGNNCC 1 cut(s) 487
BmrFI CCNGG 2 cut(s) 188, 1029
BmrI ACTGGG 1 cut(s) 596
BmsI GCATC 4 cut(s) 104, 950, 1361, 1440
BmuI ACTGGG 1 cut(s) 596
BoxI GACNNNNGTC 1 cut(s) 1465
BpiI GAAGAC 3 cut(s) 1169, 1465, 1494
BpmI CTGGAG 1 cut(s) 1436
Bpu10I CCTNAGC 1 cut(s) 1277
BpuEI CTTGAG 3 cut(s) 578, 1242, 1308
Bsa29I ATCGAT 1 cut(s) 1206
BsaAI YACGTR 1 cut(s) 458
BsaBI GATNNNNATC 1 cut(s) 175
BsaI GGTCTC 2 cut(s) 836, 929
BsaJI CCNNGG 1 cut(s) 761
BsaXI ACNNNNNCTCC 2 cut(s) 738, 768
Bsc4I CCNNNNNNNGG 2 cut(s) 83, 767
Bse1I ACTGG 5 cut(s) 483, 602, 803, 835, 1419
Bse8I GATNNNNATC 1 cut(s) 175
BseBI CCWGG 2 cut(s) 188, 1029
BseCI ATCGAT 1 cut(s) 1206
BseDI CCNNGG 1 cut(s) 761
BseGI GGATG 4 cut(s) 274, 580, 904, 1042
BseJI GATNNNNATC 1 cut(s) 175
BseLI CCNNNNNNNGG 2 cut(s) 83, 767
BseMII CTCAG 4 cut(s) 940, 1173, 1291, 1305
BseNI ACTGG 5 cut(s) 483, 602, 803, 835, 1419
BseRI GAGGAG 1 cut(s) 750
BseXI GCAGC 8 cut(s) 291, 613, 1060, 1066, 1079, 1084, 1088, 1094
Bsh1236I CGCG 1 cut(s) 1271
BshFI GGCC 1 cut(s) 168
BshVI ATCGAT 1 cut(s) 1206
BsiHKCI CYCGRG 4 cut(s) 90, 489, 762, 1382
BsiSI CCGG 2 cut(s) 160, 449
BslFI GGGAC 2 cut(s) 369, 1507
BslI CCNNNNNNNGG 2 cut(s) 83, 767
BsmAI GTCTC 5 cut(s) 520, 554, 578, 836, 929
BsmFI GGGAC 2 cut(s) 369, 1507
BsmI GAATGC 1 cut(s) 40
BsnI GGCC 1 cut(s) 168
Bso31I GGTCTC 2 cut(s) 836, 929
BsoBI CYCGRG 4 cut(s) 90, 489, 762, 1382
Bsp143I GATC 8 cut(s) 9, 196, 348, 485, 538, 616, 640, 691
BspACI CCGC 6 cut(s) 116, 391, 816, 978, 1071, 1250
BspANI GGCC 1 cut(s) 168
BspCNI CTCAG 4 cut(s) 939, 1174, 1290, 1306
BspDI ATCGAT 1 cut(s) 1206
BspFNI CGCG 1 cut(s) 1271
BspHI TCATGA 1 cut(s) 1014
BspLI GGNNCC 1 cut(s) 487
BspMAI CTGCAG 2 cut(s) 1078, 1084
BspMI ACCTGC 1 cut(s) 1150
BspPI GGATC 2 cut(s) 480, 493
BspTNI GGTCTC 2 cut(s) 836, 929
BsrI ACTGG 5 cut(s) 483, 602, 803, 835, 1419
BssECI CCNNGG 1 cut(s) 761
BssMI GATC 8 cut(s) 9, 196, 348, 485, 538, 616, 640, 691
Bst2UI CCWGG 2 cut(s) 188, 1029
Bst4CI ACNGT 5 cut(s) 689, 827, 1175, 1365, 1498
BstAPI GCANNNNNTGC 1 cut(s) 1073
BstBAI YACGTR 1 cut(s) 458
BstC8I GCNNGC 1 cut(s) 114
BstDEI CTNAG 7 cut(s) 200, 926, 964, 1182, 1277, 1314, 1454
BstENI CCTNNNNNAGG 1 cut(s) 765
BstF5I GGATG 4 cut(s) 274, 580, 904, 1042
BstFNI CGCG 1 cut(s) 1271
BstKTI GATC 8 cut(s) 12, 199, 351, 488, 541, 619, 643, 694
BstMAI GTCTC 5 cut(s) 520, 554, 578, 836, 929
BstMBI GATC 8 cut(s) 9, 196, 348, 485, 538, 616, 640, 691
BstMWI GCNNNNNNNGC 9 cut(s) 165, 299, 773, 1073, 1076, 1079, 1082, 1103, 1394
BstNI CCWGG 2 cut(s) 188, 1029
BstNSI RCATGY 3 cut(s) 116, 385, 548
BstPAI GACNNNNGTC 1 cut(s) 1465
BstSCI CCNGG 2 cut(s) 186, 1027
BstSFI CTRYAG 4 cut(s) 685, 1074, 1080, 1338
BstUI CGCG 1 cut(s) 1271
BstV1I GCAGC 8 cut(s) 291, 613, 1060, 1066, 1079, 1084, 1088, 1094
BstV2I GAAGAC 3 cut(s) 1169, 1465, 1494
BstX2I RGATCY 1 cut(s) 485
BstXI CCANNNNNNTGG 1 cut(s) 478
BstYI RGATCY 1 cut(s) 485
Bsu15I ATCGAT 1 cut(s) 1206
BsuRI GGCC 1 cut(s) 168
BsuTUI ATCGAT 1 cut(s) 1206
BtgZI GCGATG 1 cut(s) 299
BtsCI GGATG 4 cut(s) 274, 580, 904, 1042
BtsI GCAGTG 2 cut(s) 126, 1092
BtsIMutI CAGTG 7 cut(s) 126, 476, 694, 934, 1092, 1311, 1412
BveI ACCTGC 1 cut(s) 1150
Cac8I GCNNGC 1 cut(s) 114
CaiI CAGNNNCTG 3 cut(s) 1073, 1088, 1475
CciI TCATGA 1 cut(s) 1014
ClaI ATCGAT 1 cut(s) 1206
CseI GACGC 1 cut(s) 1461
Csp6I GTAC 1 cut(s) 828
CviAII CATG 8 cut(s) 113, 319, 382, 417, 545, 737, 890, 1015
CviQI GTAC 1 cut(s) 828
DdeI CTNAG 7 cut(s) 200, 926, 964, 1182, 1277, 1314, 1454
DpnI GATC 8 cut(s) 11, 198, 350, 487, 540, 618, 642, 693
DpnII GATC 8 cut(s) 9, 196, 348, 485, 538, 616, 640, 691
DraI TTTAAA 1 cut(s) 1378
DraIII CACNNNGTG 1 cut(s) 461
EaeI YGGCCR 1 cut(s) 166
Eco31I GGTCTC 2 cut(s) 836, 929
Eco57I CTGAAG 1 cut(s) 1461
Eco72I CACGTG 1 cut(s) 458
Eco88I CYCGRG 4 cut(s) 90, 489, 762, 1382
EcoNI CCTNNNNNAGG 1 cut(s) 765
EcoRII CCWGG 2 cut(s) 186, 1027
FaeI CATG 8 cut(s) 116, 322, 385, 420, 548, 740, 893, 1018
FalI AAGNNNNNCTT 4 cut(s) 196, 228, 1335, 1367
FaqI GGGAC 2 cut(s) 369, 1507
FatI CATG 8 cut(s) 112, 318, 381, 416, 544, 736, 889, 1014
FbaI TGATCA 1 cut(s) 616
FblI GTMKAC 1 cut(s) 878
FokI GGATG 4 cut(s) 281, 587, 891, 1029
FspBI CTAG 4 cut(s) 50, 219, 915, 1368
GsuI CTGGAG 1 cut(s) 1436
HaeIII GGCC 1 cut(s) 168
HapII CCGG 2 cut(s) 160, 449
HgaI GACGC 1 cut(s) 1461
Hin1II CATG 8 cut(s) 116, 322, 385, 420, 548, 740, 893, 1018
HincII GTYRAC 1 cut(s) 1349
HindII GTYRAC 1 cut(s) 1349
HindIII AAGCTT 2 cut(s) 291, 338
HinfI GANTC 7 cut(s) 239, 423, 780, 1034, 1203, 1238, 1418
HpaII CCGG 2 cut(s) 160, 449
HphI GGTGA 4 cut(s) 44, 775, 830, 1053
Hpy166II GTNNAC 3 cut(s) 464, 879, 1349
Hpy188I TCNGA 8 cut(s) 181, 310, 376, 640, 1120, 1129, 1360, 1477
Hpy8I GTNNAC 3 cut(s) 464, 879, 1349
HpyAV CCTTC 2 cut(s) 1337, 1533
HpyCH4III ACNGT 5 cut(s) 689, 827, 1175, 1365, 1498
HpyCH4IV ACGT 1 cut(s) 457
HpyCH4V TGCA 7 cut(s) 544, 712, 1076, 1082, 1145, 1405, 1501
HpyF10VI GCNNNNNNNGC 9 cut(s) 165, 299, 773, 1073, 1076, 1079, 1082, 1103, 1394
HpyF3I CTNAG 7 cut(s) 200, 926, 964, 1182, 1277, 1314, 1454
HpySE526I ACGT 1 cut(s) 457
Hsp92II CATG 8 cut(s) 116, 322, 385, 420, 548, 740, 893, 1018
Ksp22I TGATCA 1 cut(s) 616
Kzo9I GATC 8 cut(s) 9, 196, 348, 485, 538, 616, 640, 691
Lsp1109I GCAGC 8 cut(s) 291, 613, 1060, 1066, 1079, 1084, 1088, 1094
LweI GCATC 4 cut(s) 104, 950, 1361, 1440
MaeI CTAG 4 cut(s) 50, 219, 915, 1368
MaeII ACGT 1 cut(s) 457
MaeIII GTNAC 5 cut(s) 104, 726, 754, 1059, 1090
MalI GATC 8 cut(s) 11, 198, 350, 487, 540, 618, 642, 693
MboI GATC 8 cut(s) 9, 196, 348, 485, 538, 616, 640, 691
MflI RGATCY 1 cut(s) 485
MluCI AATT 1 cut(s) 1400
MlyI GAGTC 3 cut(s) 432, 1247, 1427
MmeI TCCRAC 2 cut(s) 463, 1175
MroXI GAANNNNTTC 1 cut(s) 294
MseI TTAA 4 cut(s) 324, 1056, 1215, 1377
MslI CAYNNNNRTG 4 cut(s) 386, 415, 417, 431
MspA1I CMGCKG 3 cut(s) 626, 1073, 1079
MspI CCGG 2 cut(s) 160, 449
MspR9I CCNGG 2 cut(s) 188, 1029
Mva1269I GAATGC 1 cut(s) 40
MvaI CCWGG 2 cut(s) 188, 1029
MvnI CGCG 1 cut(s) 1271
MwoI GCNNNNNNNGC 9 cut(s) 165, 299, 773, 1073, 1076, 1079, 1082, 1103, 1394
NdeII GATC 8 cut(s) 9, 196, 348, 485, 538, 616, 640, 691
NlaIII CATG 8 cut(s) 116, 322, 385, 420, 548, 740, 893, 1018
NlaIV GGNNCC 1 cut(s) 487
NmuCI GTSAC 3 cut(s) 726, 1059, 1090
NspI RCATGY 3 cut(s) 116, 385, 548
PaeI GCATGC 1 cut(s) 116
PaeR7I CTCGAG 2 cut(s) 90, 1382
PagI TCATGA 1 cut(s) 1014
PaqCI CACCTGC 1 cut(s) 1150
PciI ACATGT 1 cut(s) 381
PctI GAATGC 1 cut(s) 40
PdmI GAANNNNTTC 1 cut(s) 294
PfeI GAWTC 4 cut(s) 239, 780, 1034, 1203
PflFI GACNNNGTC 1 cut(s) 214
PleI GAGTC 3 cut(s) 431, 1246, 1426
PmaCI CACGTG 1 cut(s) 458
PmlI CACGTG 1 cut(s) 458
PpsI GAGTC 3 cut(s) 431, 1246, 1426
Ppu21I YACGTR 1 cut(s) 458
PscI ACATGT 1 cut(s) 381
PshAI GACNNNNGTC 1 cut(s) 1465
Psp6I CCWGG 2 cut(s) 186, 1027
PspCI CACGTG 1 cut(s) 458
PspGI CCWGG 2 cut(s) 186, 1027
PspN4I GGNNCC 1 cut(s) 487
PspXI VCTCGAGB 1 cut(s) 1382
PstI CTGCAG 2 cut(s) 1078, 1084
PstNI CAGNNNCTG 3 cut(s) 1073, 1088, 1475
PsuI RGATCY 1 cut(s) 485
PsyI GACNNNGTC 1 cut(s) 214
PvuII CAGCTG 2 cut(s) 626, 1079
RsaI GTAC 1 cut(s) 829
RsaNI GTAC 1 cut(s) 828
RseI CAYNNNNRTG 4 cut(s) 386, 415, 417, 431
SaqAI TTAA 4 cut(s) 324, 1056, 1215, 1377
Sau3AI GATC 8 cut(s) 9, 196, 348, 485, 538, 616, 640, 691
ScaI AGTACT 1 cut(s) 829
SchI GAGTC 3 cut(s) 432, 1247, 1427
ScrFI CCNGG 2 cut(s) 188, 1029
SfaNI GCATC 4 cut(s) 104, 950, 1361, 1440
SfcI CTRYAG 4 cut(s) 685, 1074, 1080, 1338
Sfr274I CTCGAG 2 cut(s) 90, 1382
SlaI CTCGAG 2 cut(s) 90, 1382
SmiMI CAYNNNNRTG 4 cut(s) 386, 415, 417, 431
SmlI CTYRAG 5 cut(s) 90, 557, 1257, 1323, 1382
SmoI CTYRAG 5 cut(s) 90, 557, 1257, 1323, 1382
SphI GCATGC 1 cut(s) 116
Sse9I AATT 1 cut(s) 1400
SsiI CCGC 6 cut(s) 116, 391, 816, 978, 1071, 1250
SspMI CTAG 4 cut(s) 50, 219, 915, 1368
StyD4I CCNGG 2 cut(s) 186, 1027
TaaI ACNGT 5 cut(s) 689, 827, 1175, 1365, 1498
TaiI ACGT 1 cut(s) 460
TaqI TCGA 5 cut(s) 91, 677, 1206, 1383, 1434
TasI AATT 1 cut(s) 1400
TatI WGTACW 1 cut(s) 827
TauI GCSGC 3 cut(s) 119, 981, 1073
TfiI GAWTC 4 cut(s) 239, 780, 1034, 1203
Tru1I TTAA 4 cut(s) 324, 1056, 1215, 1377
Tru9I TTAA 4 cut(s) 324, 1056, 1215, 1377
TscAI CASTG 7 cut(s) 126, 483, 694, 934, 1099, 1318, 1419
TseFI GTSAC 3 cut(s) 726, 1059, 1090
TseI GCWGC 8 cut(s) 279, 626, 1067, 1073, 1076, 1079, 1082, 1097
Tsp45I GTSAC 3 cut(s) 726, 1059, 1090
TspDTI ATGAA 7 cut(s) 75, 385, 405, 542, 686, 1308, 1494
TspRI CASTG 7 cut(s) 126, 483, 694, 934, 1099, 1318, 1419
Tth111I GACNNNGTC 1 cut(s) 214
XagI CCTNNNNNAGG 1 cut(s) 765
XbaI TCTAGA 1 cut(s) 1367
XceI RCATGY 3 cut(s) 116, 385, 548
XhoI CTCGAG 2 cut(s) 90, 1382
XmiI GTMKAC 1 cut(s) 878
XmnI GAANNNNTTC 1 cut(s) 294
XspI CTAG 4 cut(s) 50, 219, 915, 1368
ZrmI AGTACT 1 cut(s) 829
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.