pycom02g20920

acyl-CoA-binding domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0005156
Physical Location & Seq
Forward (+)
18968727 .. 18972850
4124 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g20920.1

Sequence Viewer

Length: 1548 bp
ATGGGAATCGAAGGGATTGTGAAAGATGTTGATACCAGCAATTGGCAAACAGATTTGGCCTATGACCAGTGGATAGCACTCCCTGTTTCTGGTCCACGACCACCAGCTCGGTATAAGCATGCTGCTGTAGTAGCGGATGAAAAATTATACATTATTGGTGGGAGTCGTAATGGTCGGAACCTATCTGATGTCCAGGTCTTTGACTTTAGAAATTTGTTGTGGTCTAACATTAAATTGAAACCCAATTCGGATAAATTTGAAGACAGCGGCTTACAGGAAGTTCTTCCAGGAATTTCTGCTCACAATATGGTTAGGTGGGGAAATAAACTTCTTCTTCTTGGTGGAAATTCAAGGAAATCGTCTGATAGAATAATAGTTTGGTTCATCGATTTGGAGACGCATGTCTGTGGCATCATCGAGACCTCAGGAAACGTTCCAGTAGCTCGTGGGGGCCAGTCCACCACACTGGTTGGTTCTAGACTGATAATGTTTGGTGGAGAAGACAGGAGCAGAAGATTGTTGAATGATGTTAATGTTCTTGATTTAGAGACAATGACTTGGGATGTGATGGAAGCAACGCAGACACCTCCACCTCCCAGATTCGATCACGCAGCTGCAGTACATGCAGAACGCTACCTTCTAATTTTTGGTGGTTGTTCTCATGCTACTTTCTTCAATGATCTTCATGTACTTGACCTGCAAGCAAGGGAGTGGTCCCAACCACAAATTCAGGGTGATCTAGTGACTCCTAGGGCTGGTCATGCTGGTATAACCATTGATGAAAACTGGTATATAGTTGGCGGTGGAGATAACAAAAATGGCTGCCCCGAAACTCTGGTGTTAAATATGTCTAAGCTAGTGTGGTCAGTCTCAACCAGTGTGAAGCAAAGGGATGCACTCGCTAGTGAGGGACTCAGTGTCTGCTCAGTTACTATAGATGGAAAGAAGCATTTGGTTGCCTTTGGTGGCTATAATGGGAAGTATAGCAATGAGGTTTTCGTAATGATGCCCAAACCGAGGGACTTATCACAACCCAAGATATTCCAGTCACCAGCAGCGGCTGCAGCAGCAGCTTCTGTTACTGCTGCATATGCCTTATCTAAAATAGAAAATTTGGATTTCAAAACAGCAGAGCTGAACTCCAAGGGACCTGGAAACCATCATTATGAACAAGATTTCACGGTTGAGATAGAGGCCCTTACAGAAGAGAAAAGTGTGCTGGAGTTGTCGCTGGCGGAAGTCAGGACAGAAAATTCCAGGATTGGGCAGAATATTGAAGAAGTCAACAATACTCATGCTGAGTTGTCCAAGGAACTTCTTTCAGTCCAAGGGCAACTAGTAGCTGAGAGATCAAGATGCTTTAAATTGGAGGCTCAAATCGGTGAACTGCAAAAGAAATTAGAATCAGTGCAGTCCATTGAGGATGAGGTGCAGGCACTTCGGGGTCAAAAATCTGCATTCGAACGGGATGAGCTTGCCTCATCTGTTCAAAGACAAGGTTCTGGTGGGGTTTGGCGGTGGATTGCTGGGGGTGGTGGCGACTCATAA

Protein Analysis

516

Amino Acids

56.57

Weight (kDa)

5.43

Isoelectric Point (pI)

42.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_ATRN-LZTR1 PF24981 14 - 155 6.5e-13 Attractin/LZTR1 beta-propeller
Kelch_KLHDC2_KLHL20_DRC7 PF24681 15 - 80 1.1e-12 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 21 - 80 1.5e-08 FBX42, beta-propeller domain
Kelch_1 PF01344 36 - 77 2.5e-09 Kelch motif
Kelch_HCF PF13854 93 - 268 4e-16 Host cell factor, Kelch-repeats domain
Beta-prop_FBX42 PF13415 98 - 222 3.7e-24 FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 101 - 308 7.1e-51 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 112 - 268 1.2e-19 Attractin/LZTR1 beta-propeller
Beta-prop_ATRN-LZTR1 PF24981 162 - 328 1.1e-19 Attractin/LZTR1 beta-propeller
Beta-prop_FBX42 PF13415 200 - 359 1.8e-22 FBX42, beta-propeller domain
ACBP4_C PF24922 394 - 497 3.2e-38 Acyl-CoA-binding domain-containing protein 4-6, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 705
AccB7I CCANNNNNTGG 1 cut(s) 42
AciI CCGC 6 cut(s) 134, 267, 801, 1058, 1235, 1516
AclI AACGTT 1 cut(s) 432
AcsI RAATTY 7 cut(s) 211, 254, 291, 346, 726, 1111, 1252
AfaI GTAC 2 cut(s) 621, 690
AfiI CCNNNNNNNGG 5 cut(s) 42, 89, 755, 1017, 1263
AgsI TTSAA 8 cut(s) 238, 260, 351, 523, 676, 1123, 1277, 1490
AhdI GACNNNNNGTC 1 cut(s) 917
AhlI ACTAGT 1 cut(s) 1336
AjnI CCWGG 4 cut(s) 192, 286, 1150, 1256
AjuI GAANNNNNNNTTGG 4 cut(s) 361, 393, 711, 743
AluBI AGCT 8 cut(s) 107, 443, 614, 856, 1073, 1135, 1343, 1474
AluI AGCT 8 cut(s) 107, 443, 614, 856, 1073, 1135, 1343, 1474
Alw26I GTCTC 4 cut(s) 389, 413, 542, 874
AlwNI CAGNNNCTG 3 cut(s) 921, 1061, 1076
AoxI GGCC 3 cut(s) 57, 451, 1194
ApoI RAATTY 7 cut(s) 211, 254, 291, 346, 726, 1111, 1252
Asp700I GAANNNNTTC 1 cut(s) 282
AspA2I CCTAGG 1 cut(s) 749
AspS9I GGNCC 5 cut(s) 92, 451, 714, 1148, 1195
AsuHPI GGTGA 3 cut(s) 746, 1041, 1394
AsuII TTCGAA 1 cut(s) 1461
AvaII GGWCC 3 cut(s) 92, 714, 1148
AvrII CCTAGG 1 cut(s) 749
AxyI CCTNAGG 1 cut(s) 424
BauI CACGAG 1 cut(s) 444
BbsI GAAGAC 2 cut(s) 267, 507
BccI CCATC 3 cut(s) 562, 932, 1167
BcgI CGANNNNNNTGC 2 cut(s) 1421, 1455
BciT130I CCWGG 4 cut(s) 194, 288, 1152, 1258
BcoDI GTCTC 4 cut(s) 389, 413, 542, 874
BcuI ACTAGT 1 cut(s) 1336
BfaI CTAG 6 cut(s) 477, 740, 750, 857, 903, 1337
BfmI CTRYAG 4 cut(s) 126, 615, 933, 1062
BfuAI ACCTGC 1 cut(s) 705
BlnI CCTAGG 1 cut(s) 749
Bme1390I CCNGG 4 cut(s) 194, 288, 1152, 1258
Bme18I GGWCC 3 cut(s) 92, 714, 1148
BmeRI GACNNNNNGTC 1 cut(s) 917
BmgT120I GGNCC 5 cut(s) 92, 451, 714, 1148, 1195
BmiI GGNNCC 4 cut(s) 179, 452, 716, 1149
BmrFI CCNGG 4 cut(s) 194, 288, 1152, 1258
BmsI GCATC 4 cut(s) 420, 883, 996, 1346
BoxI GACNNNNGTC 1 cut(s) 401
BpiI GAAGAC 2 cut(s) 267, 507
BplI GAGNNNNNCTC 4 cut(s) 1124, 1156, 1463, 1495
BpmI CTGGAG 1 cut(s) 1241
Bpu14I TTCGAA 1 cut(s) 1461
Bsa29I ATCGAT 1 cut(s) 387
BsaI GGTCTC 1 cut(s) 413
BsaJI CCNNGG 5 cut(s) 749, 1016, 1143, 1308, 1327
Bsc4I CCNNNNNNNGG 5 cut(s) 42, 89, 755, 1017, 1263
Bse1I ACTGG 7 cut(s) 67, 437, 454, 471, 791, 876, 1045
Bse21I CCTNAGG 1 cut(s) 424
Bse3DI GCAATG 1 cut(s) 994
BseBI CCWGG 4 cut(s) 194, 288, 1152, 1258
BseCI ATCGAT 1 cut(s) 387
BseDI CCNNGG 5 cut(s) 749, 1016, 1143, 1308, 1327
BseGI GGATG 5 cut(s) 142, 568, 898, 1429, 1474
BseLI CCNNNNNNNGG 5 cut(s) 42, 89, 755, 1017, 1263
BseMI GCAATG 1 cut(s) 994
BseMII CTCAG 5 cut(s) 438, 928, 939, 1290, 1335
BseNI ACTGG 7 cut(s) 67, 437, 454, 471, 791, 876, 1045
BseYI CCCAGC 1 cut(s) 1526
BsgI GTGCAG 2 cut(s) 1430, 1451
BshFI GGCC 3 cut(s) 59, 453, 1196
BshVI ATCGAT 1 cut(s) 387
BslFI GGGAC 4 cut(s) 700, 924, 1034, 1161
BslI CCNNNNNNNGG 5 cut(s) 42, 89, 755, 1017, 1263
BsmAI GTCTC 4 cut(s) 389, 413, 542, 874
BsmBI CGTCTC 1 cut(s) 389
BsmFI GGGAC 4 cut(s) 700, 924, 1034, 1161
BsmI GAATGC 1 cut(s) 1457
BsnI GGCC 3 cut(s) 59, 453, 1196
Bso31I GGTCTC 1 cut(s) 413
Bsp119I TTCGAA 1 cut(s) 1461
Bsp143I GATC 4 cut(s) 604, 679, 736, 1349
BspACI CCGC 6 cut(s) 134, 267, 801, 1058, 1235, 1516
BspANI GGCC 3 cut(s) 59, 453, 1196
BspCNI CTCAG 5 cut(s) 437, 927, 938, 1291, 1336
BspDI ATCGAT 1 cut(s) 387
BspLI GGNNCC 4 cut(s) 179, 452, 716, 1149
BspMAI CTGCAG 2 cut(s) 619, 1066
BspMI ACCTGC 1 cut(s) 705
BspT104I TTCGAA 1 cut(s) 1461
BspTNI GGTCTC 1 cut(s) 413
BsrDI GCAATG 1 cut(s) 994
BsrI ACTGG 7 cut(s) 67, 437, 454, 471, 791, 876, 1045
BssECI CCNNGG 5 cut(s) 749, 1016, 1143, 1308, 1327
BssMI GATC 4 cut(s) 604, 679, 736, 1349
BssSI CACGAG 1 cut(s) 444
BssT1I CCWWGG 4 cut(s) 749, 1143, 1308, 1327
Bst2BI CACGAG 1 cut(s) 444
Bst2UI CCWGG 4 cut(s) 194, 288, 1152, 1258
Bst4CI ACNGT 1 cut(s) 1183
Bst6I CTCTTC 1 cut(s) 1200
BstAPI GCANNNNNTGC 2 cut(s) 623, 1061
BstBI TTCGAA 1 cut(s) 1461
BstC8I GCNNGC 5 cut(s) 120, 702, 1233, 1434, 1476
BstDEI CTNAG 6 cut(s) 424, 852, 914, 925, 1299, 1344
BstF5I GGATG 5 cut(s) 142, 568, 898, 1429, 1474
BstKTI GATC 4 cut(s) 607, 682, 739, 1352
BstMAI GTCTC 4 cut(s) 389, 413, 542, 874
BstMBI GATC 4 cut(s) 604, 679, 736, 1349
BstMWI GCNNNNNNNGC 8 cut(s) 131, 623, 761, 1061, 1064, 1067, 1070, 1091
BstNI CCWGG 4 cut(s) 194, 288, 1152, 1258
BstNSI RCATGY 3 cut(s) 122, 404, 626
BstPAI GACNNNNGTC 1 cut(s) 401
BstSCI CCNGG 4 cut(s) 192, 286, 1150, 1256
BstSFI CTRYAG 4 cut(s) 126, 615, 933, 1062
BstV2I GAAGAC 2 cut(s) 267, 507
BstXI CCANNNNNNTGG 1 cut(s) 466
Bsu15I ATCGAT 1 cut(s) 387
Bsu36I CCTNAGG 1 cut(s) 424
BsuRI GGCC 3 cut(s) 59, 453, 1196
BsuTUI ATCGAT 1 cut(s) 387
BtsCI GGATG 5 cut(s) 142, 568, 898, 1429, 1474
BtsIMutI CAGTG 5 cut(s) 74, 464, 883, 922, 1413
BveI ACCTGC 1 cut(s) 705
Cac8I GCNNGC 5 cut(s) 120, 702, 1233, 1434, 1476
CaiI CAGNNNCTG 3 cut(s) 921, 1061, 1076
Cfr13I GGNCC 5 cut(s) 92, 451, 714, 1148, 1195
ClaI ATCGAT 1 cut(s) 387
CseI GACGC 1 cut(s) 406
Csp6I GTAC 2 cut(s) 620, 689
CspCI CAANNNNNGTGG 2 cut(s) 451, 486
CviAII CATG 7 cut(s) 119, 401, 623, 662, 686, 761, 1295
CviQI GTAC 2 cut(s) 620, 689
DdeI CTNAG 6 cut(s) 424, 852, 914, 925, 1299, 1344
DpnI GATC 4 cut(s) 606, 681, 738, 1351
DpnII GATC 4 cut(s) 604, 679, 736, 1349
DraI TTTAAA 1 cut(s) 1363
DriI GACNNNNNGTC 1 cut(s) 917
Eam1104I CTCTTC 1 cut(s) 1200
Eam1105I GACNNNNNGTC 1 cut(s) 917
EarI CTCTTC 1 cut(s) 1200
EciI GGCGGA 1 cut(s) 1250
Eco130I CCWWGG 4 cut(s) 749, 1143, 1308, 1327
Eco31I GGTCTC 1 cut(s) 413
Eco47I GGWCC 3 cut(s) 92, 714, 1148
Eco81I CCTNAGG 1 cut(s) 424
EcoO109I RGGNCCY 2 cut(s) 1148, 1195
EcoRII CCWGG 4 cut(s) 192, 286, 1150, 1256
EcoT14I CCWWGG 4 cut(s) 749, 1143, 1308, 1327
ErhI CCWWGG 4 cut(s) 749, 1143, 1308, 1327
Esp3I CGTCTC 1 cut(s) 389
FaeI CATG 7 cut(s) 122, 404, 626, 665, 689, 764, 1298
FaqI GGGAC 4 cut(s) 700, 924, 1034, 1161
FatI CATG 7 cut(s) 118, 400, 622, 661, 685, 760, 1294
FauNDI CATATG 1 cut(s) 1090
FokI GGATG 5 cut(s) 149, 575, 905, 1436, 1481
FspBI CTAG 6 cut(s) 477, 740, 750, 857, 903, 1337
GsaI CCCAGC 1 cut(s) 1530
GsuI CTGGAG 1 cut(s) 1241
HaeIII GGCC 3 cut(s) 59, 453, 1196
HgaI GACGC 1 cut(s) 406
Hin1II CATG 7 cut(s) 122, 404, 626, 665, 689, 764, 1298
HincII GTYRAC 1 cut(s) 1285
HindII GTYRAC 1 cut(s) 1285
HinfI GANTC 7 cut(s) 6, 163, 600, 745, 912, 1403, 1541
HphI GGTGA 3 cut(s) 746, 1041, 1394
Hpy166II GTNNAC 4 cut(s) 95, 459, 1285, 1385
Hpy188I TCNGA 4 cut(s) 177, 187, 250, 364
Hpy188III TCNNGA 6 cut(s) 418, 426, 477, 539, 1243, 1353
Hpy8I GTNNAC 4 cut(s) 95, 459, 1285, 1385
HpyAV CCTTC 2 cut(s) 5, 647
HpyCH4III ACNGT 1 cut(s) 1183
HpyCH4IV ACGT 1 cut(s) 432
HpyF10VI GCNNNNNNNGC 8 cut(s) 131, 623, 761, 1061, 1064, 1067, 1070, 1091
HpyF3I CTNAG 6 cut(s) 424, 852, 914, 925, 1299, 1344
HpySE526I ACGT 1 cut(s) 432
Hsp92II CATG 7 cut(s) 122, 404, 626, 665, 689, 764, 1298
Kzo9I GATC 4 cut(s) 604, 679, 736, 1349
LmnI GCTCC 1 cut(s) 507
LweI GCATC 4 cut(s) 420, 883, 996, 1346
MaeI CTAG 6 cut(s) 477, 740, 750, 857, 903, 1337
MaeII ACGT 1 cut(s) 432
MaeIII GTNAC 4 cut(s) 742, 928, 1047, 1078
MalI GATC 4 cut(s) 606, 681, 738, 1351
MboI GATC 4 cut(s) 604, 679, 736, 1349
MfeI CAATTG 1 cut(s) 40
MlyI GAGTC 4 cut(s) 172, 739, 906, 1535
MmeI TCCRAC 1 cut(s) 155
MroXI GAANNNNTTC 1 cut(s) 282
MseI TTAA 4 cut(s) 231, 531, 842, 1362
MslI CAYNNNNRTG 2 cut(s) 405, 1164
MspA1I CMGCKG 3 cut(s) 267, 614, 1058
MspR9I CCNGG 4 cut(s) 194, 288, 1152, 1258
MunI CAATTG 1 cut(s) 40
Mva1269I GAATGC 1 cut(s) 1457
MvaI CCWGG 4 cut(s) 194, 288, 1152, 1258
MwoI GCNNNNNNNGC 8 cut(s) 131, 623, 761, 1061, 1064, 1067, 1070, 1091
NdeI CATATG 1 cut(s) 1090
NdeII GATC 4 cut(s) 604, 679, 736, 1349
NlaIII CATG 7 cut(s) 122, 404, 626, 665, 689, 764, 1298
NlaIV GGNNCC 4 cut(s) 179, 452, 716, 1149
NmuCI GTSAC 2 cut(s) 742, 1047
NspI RCATGY 3 cut(s) 122, 404, 626
NspV TTCGAA 1 cut(s) 1461
PaeI GCATGC 1 cut(s) 122
PctI GAATGC 1 cut(s) 1457
PdmI GAANNNNTTC 1 cut(s) 282
PfeI GAWTC 3 cut(s) 6, 600, 1403
PflMI CCANNNNNTGG 1 cut(s) 42
PfoI TCCNGGA 2 cut(s) 286, 1256
PleI GAGTC 4 cut(s) 171, 739, 906, 1535
PpsI GAGTC 4 cut(s) 171, 739, 906, 1535
PpuMI RGGWCCY 1 cut(s) 1148
PshAI GACNNNNGTC 1 cut(s) 401
Psp1406I AACGTT 1 cut(s) 432
Psp5II RGGWCCY 1 cut(s) 1148
Psp6I CCWGG 4 cut(s) 192, 286, 1150, 1256
PspFI CCCAGC 1 cut(s) 1526
PspGI CCWGG 4 cut(s) 192, 286, 1150, 1256
PspN4I GGNNCC 4 cut(s) 179, 452, 716, 1149
PspPI GGNCC 5 cut(s) 92, 451, 714, 1148, 1195
PspPPI RGGWCCY 1 cut(s) 1148
PstI CTGCAG 2 cut(s) 619, 1066
PstNI CAGNNNCTG 3 cut(s) 921, 1061, 1076
PvuII CAGCTG 1 cut(s) 614
RsaI GTAC 2 cut(s) 621, 690
RsaNI GTAC 2 cut(s) 620, 689
RseI CAYNNNNRTG 2 cut(s) 405, 1164
SaqAI TTAA 4 cut(s) 231, 531, 842, 1362
Sau3AI GATC 4 cut(s) 604, 679, 736, 1349
Sau96I GGNCC 5 cut(s) 92, 451, 714, 1148, 1195
SchI GAGTC 4 cut(s) 172, 739, 906, 1535
ScrFI CCNGG 4 cut(s) 194, 288, 1152, 1258
SfaNI GCATC 4 cut(s) 420, 883, 996, 1346
SfcI CTRYAG 4 cut(s) 126, 615, 933, 1062
SfuI TTCGAA 1 cut(s) 1461
SinI GGWCC 3 cut(s) 92, 714, 1148
SmiMI CAYNNNNRTG 2 cut(s) 405, 1164
SpeI ACTAGT 1 cut(s) 1336
SphI GCATGC 1 cut(s) 122
SsiI CCGC 6 cut(s) 134, 267, 801, 1058, 1235, 1516
SspI AATATT 1 cut(s) 1273
SspMI CTAG 6 cut(s) 477, 740, 750, 857, 903, 1337
StyD4I CCNGG 4 cut(s) 192, 286, 1150, 1256
StyI CCWWGG 4 cut(s) 749, 1143, 1308, 1327
TaaI ACNGT 1 cut(s) 1183
TaiI ACGT 1 cut(s) 435
TaqI TCGA 5 cut(s) 9, 387, 417, 603, 1461
TatI WGTACW 2 cut(s) 619, 688
TauI GCSGC 2 cut(s) 270, 1061
TfiI GAWTC 3 cut(s) 6, 600, 1403
Tru1I TTAA 4 cut(s) 231, 531, 842, 1362
Tru9I TTAA 4 cut(s) 231, 531, 842, 1362
TscAI CASTG 5 cut(s) 74, 471, 883, 922, 1413
TseFI GTSAC 2 cut(s) 742, 1047
Tsp45I GTSAC 2 cut(s) 742, 1047
TspDTI ATGAA 5 cut(s) 153, 373, 674, 795, 1182
TspRI CASTG 5 cut(s) 74, 471, 883, 922, 1413
Van91I CCANNNNNTGG 1 cut(s) 42
VpaK11BI GGWCC 3 cut(s) 92, 714, 1148
XapI RAATTY 7 cut(s) 211, 254, 291, 346, 726, 1111, 1252
XbaI TCTAGA 1 cut(s) 476
XceI RCATGY 3 cut(s) 122, 404, 626
XmaJI CCTAGG 1 cut(s) 749
XmnI GAANNNNTTC 1 cut(s) 282
XspI CTAG 6 cut(s) 477, 740, 750, 857, 903, 1337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.