MD02G1242500.v1.1

acyl-CoA-binding domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0005156
Physical Location & Seq
Forward (+)
29265188 .. 29279259
14072 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1242500.v1.1.491

Sequence Viewer

Length: 1560 bp
ATGACAGCTTCTATGGGAATCGAAGGGATTGTGAAAGATGTTGATACCAGCAATTGGCAAACAGATTTGGCCTATGACCAGTGGATAGCACTCCCTGTTTCTGGTCCACGACCACCAGCTCGGTATAAGCATGCTGCTGTAGTCGTGGATGAAAAATTATACATTATTGGTGGGAGTCGTAATGGTCGGAACCTATCTGATGTTCAGGTCTTTGACTTTAGAAATTTGTTGTGGTCTAACATTAAACTGAAACCCAATTTGGATAAATTTGAAGACAGCGGCTTACAGGAAGTTCTTCCAGGAATTTCTGCTCACAATATGGTTAGGTGGGGAAATAAACTTCTTCTTCTTGGTGGAAATTCAAAGAAATCGTCTGATAGAATAATAGTTTGGTTCATCGATTTGGAAACGCATGTCTGTGGCATCATCGAGGCCTCAGGAAATGTTCCGGTAGCTCGTGGGGGCCAGTCCACCACACTGGTTGGTTCTAGACTGATAATGTTTGGTGGAGAAGACAGGAGCAGAAGATTGTTGAATGATGTTAATGTTCTTGATTTAGAAACAATGACTTGGGATGTGATGGAAGCAACGCAGACACCTCCGGCTCCCAGATTCGATCACACAGCTGCAGTACATGCAGAACGCTACCTTCTAATTTTTGGTGGTTGTTCTCATGCTACTTTCTTCAATGATCTTCATGTACTTGACTTGCAAGCAAGGGAGTGGTCCCAACCACAAATTCAGGGTGATCTAGTGGCTCCTAGGGCTGGTCATGCTGGTATAACCATTGATGAAAACTGGTATATAGTTGGCGGTGGAGATAACAAAAATGGCTGCCCCGAAACTCTGGTGTTAAATATGTCTAAGCTAGTGTGGTCAGTCTCAACCAGTGTGAAGCAAAGGGATGCACTCGCTAGTGAGGGACTCAGTGTCTGCTCAGTGACTATAGATGGAAAGAAGCATTTGGTTGCCTTCGGTGGCTATAATGGGAAGTATAGCAATGAGGTTTTTGTAATGATGCCCAAACCGAGGGACTCATCGCAACGCAAGATATTCCAGTCACCAGCAGCGGCTGCTGCAGCAGCTTCTGTTACTGCTGCATATGCCTTATCTAAAATAGAAAATTTGGATTTCAAAACAGCAGAGCTGAACTCCAAGGGACCTGAAAGCCATCATTATGAACAAGATTTCACGGTTGAGATAGAGGCCCTTACAGAGGAGAAAAGTGTGCTGGAGTTGTCGCTGGCAGAAGTCAGGACAGGAAATTCCAGGATCGGGCAGAATATTGAAGAAGTCAACAATACTCATGCTGAGTTGTCCAAGGAACTTCTTTCAGTCCAAGGGCAACTAGTAGCTGAGAGATCAAGATGCTTTCAATTGGAGGCTCAAATCAGTGAACTGCAAAAGAAATTAGAATCGGTGCAGTCCATTGAGGATGAGGTGCAGGCACTTCGGGGTCAAAAATCTGCATTCGAACGGGATGAGCTTGCCTCATCTGTTCAAAGACAAGGTTCTGGTGGGGTTTGGCGGTGGATTGCTGGGGGTGGTGGCGACTCATAA

Protein Analysis

520

Amino Acids

56.96

Weight (kDa)

5.43

Isoelectric Point (pI)

43.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_ATRN-LZTR1 PF24981 18 - 157 3.8e-12 Attractin/LZTR1 beta-propeller
Kelch_KLHDC2_KLHL20_DRC7 PF24681 19 - 84 6.4e-13 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 25 - 84 4.4e-09 FBX42, beta-propeller domain
Kelch_1 PF01344 40 - 81 2.5e-10 Kelch motif
Kelch_HCF PF13854 97 - 272 7.7e-17 Host cell factor, Kelch-repeats domain
Beta-prop_FBX42 PF13415 102 - 226 2.4e-24 FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 105 - 327 2.3e-52 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 116 - 259 5.2e-19 Attractin/LZTR1 beta-propeller
Beta-prop_ATRN-LZTR1 PF24981 166 - 334 5.1e-20 Attractin/LZTR1 beta-propeller
Beta-prop_TYW4 PF13418 201 - 356 3e-06 tRNA wybutosine-synthesizing protein 4, beta-propeller
Beta-prop_FBX42 PF13415 204 - 358 4.5e-21 FBX42, beta-propeller domain
Beta-prop_ATRN-LZTR1 PF24981 229 - 340 4.4e-09 Attractin/LZTR1 beta-propeller
ACBP4_C PF24922 398 - 501 1.6e-37 Acyl-CoA-binding domain-containing protein 4-6, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 54
AciI CCGC 4 cut(s) 279, 813, 1070, 1528
AclWI GGATC 1 cut(s) 1280
AcsI RAATTY 7 cut(s) 223, 266, 303, 358, 738, 1123, 1264
AfaI GTAC 2 cut(s) 633, 702
AfiI CCNNNNNNNGG 6 cut(s) 54, 101, 767, 1029, 1216, 1275
AgsI TTSAA 8 cut(s) 272, 363, 535, 688, 1135, 1289, 1376, 1502
AhdI GACNNNNNGTC 1 cut(s) 929
AhlI ACTAGT 1 cut(s) 1348
AjnI CCWGG 2 cut(s) 298, 1268
AjuI GAANNNNNNNTTGG 6 cut(s) 242, 274, 373, 405, 723, 755
AluBI AGCT 9 cut(s) 8, 119, 455, 626, 868, 1085, 1147, 1355, 1486
AluI AGCT 9 cut(s) 8, 119, 455, 626, 868, 1085, 1147, 1355, 1486
Alw26I GTCTC 1 cut(s) 886
AlwI GGATC 1 cut(s) 1280
AlwNI CAGNNNCTG 3 cut(s) 933, 1073, 1088
AoxI GGCC 4 cut(s) 69, 432, 463, 1206
ApeKI GCWGC 9 cut(s) 134, 626, 834, 1067, 1073, 1076, 1079, 1082, 1097
ApoI RAATTY 7 cut(s) 223, 266, 303, 358, 738, 1123, 1264
ArsI GACNNNNNNTTYG 2 cut(s) 356, 388
Asp700I GAANNNNTTC 1 cut(s) 294
AspA2I CCTAGG 1 cut(s) 761
AspS9I GGNCC 5 cut(s) 104, 463, 726, 1160, 1207
AsuHPI GGTGA 2 cut(s) 758, 1053
AsuII TTCGAA 1 cut(s) 1473
AvaII GGWCC 3 cut(s) 104, 726, 1160
AvrII CCTAGG 1 cut(s) 761
AxyI CCTNAGG 1 cut(s) 436
BauI CACGAG 1 cut(s) 456
BbsI GAAGAC 2 cut(s) 279, 519
BbvI GCAGC 9 cut(s) 121, 613, 821, 1060, 1063, 1079, 1084, 1091, 1094
BccI CCATC 3 cut(s) 574, 944, 1179
BcgI CGANNNNNNTGC 2 cut(s) 1433, 1467
BciT130I CCWGG 2 cut(s) 300, 1270
BcoDI GTCTC 1 cut(s) 886
BcuI ACTAGT 1 cut(s) 1348
BfaI CTAG 6 cut(s) 489, 752, 762, 869, 915, 1349
BfmI CTRYAG 4 cut(s) 138, 627, 945, 1077
BlnI CCTAGG 1 cut(s) 761
Bme1390I CCNGG 2 cut(s) 300, 1270
Bme18I GGWCC 3 cut(s) 104, 726, 1160
BmeRI GACNNNNNGTC 1 cut(s) 929
BmgT120I GGNCC 5 cut(s) 104, 463, 726, 1160, 1207
BmiI GGNNCC 6 cut(s) 191, 464, 606, 728, 759, 1161
BmrFI CCNGG 2 cut(s) 300, 1270
BmsI GCATC 4 cut(s) 432, 895, 1008, 1358
BpiI GAAGAC 2 cut(s) 279, 519
BplI GAGNNNNNCTC 4 cut(s) 1136, 1168, 1475, 1507
BpmI CTGGAG 1 cut(s) 1253
Bpu14I TTCGAA 1 cut(s) 1473
Bsa29I ATCGAT 1 cut(s) 399
BsaJI CCNNGG 5 cut(s) 761, 1028, 1155, 1320, 1339
BsaWI WCCGGW 1 cut(s) 448
Bsc4I CCNNNNNNNGG 6 cut(s) 54, 101, 767, 1029, 1216, 1275
Bse1I ACTGG 6 cut(s) 79, 466, 483, 803, 888, 1057
Bse21I CCTNAGG 1 cut(s) 436
Bse3DI GCAATG 1 cut(s) 1006
BseBI CCWGG 2 cut(s) 300, 1270
BseCI ATCGAT 1 cut(s) 399
BseDI CCNNGG 5 cut(s) 761, 1028, 1155, 1320, 1339
BseGI GGATG 5 cut(s) 154, 580, 910, 1441, 1486
BseLI CCNNNNNNNGG 6 cut(s) 54, 101, 767, 1029, 1216, 1275
BseMI GCAATG 1 cut(s) 1006
BseMII CTCAG 5 cut(s) 450, 940, 951, 1302, 1347
BseNI ACTGG 6 cut(s) 79, 466, 483, 803, 888, 1057
BseRI GAGGAG 1 cut(s) 1232
BseXI GCAGC 9 cut(s) 121, 613, 821, 1060, 1063, 1079, 1084, 1091, 1094
BseYI CCCAGC 1 cut(s) 1538
BsgI GTGCAG 2 cut(s) 1442, 1463
BshFI GGCC 4 cut(s) 71, 434, 465, 1208
BshVI ATCGAT 1 cut(s) 399
BsiSI CCGG 2 cut(s) 449, 602
BslFI GGGAC 4 cut(s) 712, 936, 1046, 1173
BslI CCNNNNNNNGG 6 cut(s) 54, 101, 767, 1029, 1216, 1275
BsmAI GTCTC 1 cut(s) 886
BsmFI GGGAC 4 cut(s) 712, 936, 1046, 1173
BsmI GAATGC 1 cut(s) 1469
BsnI GGCC 4 cut(s) 71, 434, 465, 1208
Bsp119I TTCGAA 1 cut(s) 1473
Bsp143I GATC 5 cut(s) 616, 691, 748, 1272, 1361
BspACI CCGC 4 cut(s) 279, 813, 1070, 1528
BspANI GGCC 4 cut(s) 71, 434, 465, 1208
BspCNI CTCAG 5 cut(s) 449, 939, 950, 1303, 1348
BspDI ATCGAT 1 cut(s) 399
BspLI GGNNCC 6 cut(s) 191, 464, 606, 728, 759, 1161
BspMAI CTGCAG 2 cut(s) 631, 1081
BspPI GGATC 1 cut(s) 1280
BspT104I TTCGAA 1 cut(s) 1473
BsrDI GCAATG 1 cut(s) 1006
BsrI ACTGG 6 cut(s) 79, 466, 483, 803, 888, 1057
BssECI CCNNGG 5 cut(s) 761, 1028, 1155, 1320, 1339
BssMI GATC 5 cut(s) 616, 691, 748, 1272, 1361
BssSI CACGAG 1 cut(s) 456
BssT1I CCWWGG 4 cut(s) 761, 1155, 1320, 1339
Bst2BI CACGAG 1 cut(s) 456
Bst2UI CCWGG 2 cut(s) 300, 1270
Bst4CI ACNGT 1 cut(s) 1195
BstAPI GCANNNNNTGC 2 cut(s) 635, 1073
BstBI TTCGAA 1 cut(s) 1473
BstC8I GCNNGC 5 cut(s) 132, 714, 1245, 1446, 1488
BstDEI CTNAG 6 cut(s) 436, 864, 926, 937, 1311, 1356
BstENI CCTNNNNNAGG 1 cut(s) 1214
BstF5I GGATG 5 cut(s) 154, 580, 910, 1441, 1486
BstKTI GATC 5 cut(s) 619, 694, 751, 1275, 1364
BstMAI GTCTC 1 cut(s) 886
BstMBI GATC 5 cut(s) 616, 691, 748, 1272, 1361
BstMWI GCNNNNNNNGC 8 cut(s) 635, 764, 773, 1073, 1076, 1079, 1082, 1103
BstNI CCWGG 2 cut(s) 300, 1270
BstNSI RCATGY 3 cut(s) 134, 416, 638
BstSCI CCNGG 2 cut(s) 298, 1268
BstSFI CTRYAG 4 cut(s) 138, 627, 945, 1077
BstV1I GCAGC 9 cut(s) 121, 613, 821, 1060, 1063, 1079, 1084, 1091, 1094
BstV2I GAAGAC 2 cut(s) 279, 519
BstXI CCANNNNNNTGG 1 cut(s) 478
Bsu15I ATCGAT 1 cut(s) 399
Bsu36I CCTNAGG 1 cut(s) 436
BsuRI GGCC 4 cut(s) 71, 434, 465, 1208
BsuTUI ATCGAT 1 cut(s) 399
BtgZI GCGATG 1 cut(s) 1023
BtsCI GGATG 5 cut(s) 154, 580, 910, 1441, 1486
BtsIMutI CAGTG 6 cut(s) 86, 476, 895, 934, 945, 1399
Cac8I GCNNGC 5 cut(s) 132, 714, 1245, 1446, 1488
CaiI CAGNNNCTG 3 cut(s) 933, 1073, 1088
Cfr13I GGNCC 5 cut(s) 104, 463, 726, 1160, 1207
ClaI ATCGAT 1 cut(s) 399
Csp6I GTAC 2 cut(s) 632, 701
CspCI CAANNNNNGTGG 2 cut(s) 463, 498
CviAII CATG 7 cut(s) 131, 413, 635, 674, 698, 773, 1307
CviQI GTAC 2 cut(s) 632, 701
DdeI CTNAG 6 cut(s) 436, 864, 926, 937, 1311, 1356
DpnI GATC 5 cut(s) 618, 693, 750, 1274, 1363
DpnII GATC 5 cut(s) 616, 691, 748, 1272, 1361
DriI GACNNNNNGTC 1 cut(s) 929
Eam1105I GACNNNNNGTC 1 cut(s) 929
Eco130I CCWWGG 4 cut(s) 761, 1155, 1320, 1339
Eco147I AGGCCT 1 cut(s) 434
Eco47I GGWCC 3 cut(s) 104, 726, 1160
Eco81I CCTNAGG 1 cut(s) 436
EcoNI CCTNNNNNAGG 1 cut(s) 1214
EcoO109I RGGNCCY 2 cut(s) 1160, 1207
EcoRII CCWGG 2 cut(s) 298, 1268
EcoT14I CCWWGG 4 cut(s) 761, 1155, 1320, 1339
ErhI CCWWGG 4 cut(s) 761, 1155, 1320, 1339
FaeI CATG 7 cut(s) 134, 416, 638, 677, 701, 776, 1310
FaqI GGGAC 4 cut(s) 712, 936, 1046, 1173
FatI CATG 7 cut(s) 130, 412, 634, 673, 697, 772, 1306
FauNDI CATATG 1 cut(s) 1102
FokI GGATG 5 cut(s) 161, 587, 917, 1448, 1493
FspBI CTAG 6 cut(s) 489, 752, 762, 869, 915, 1349
GsaI CCCAGC 1 cut(s) 1542
GsuI CTGGAG 1 cut(s) 1253
HaeIII GGCC 4 cut(s) 71, 434, 465, 1208
HapII CCGG 2 cut(s) 449, 602
Hin1II CATG 7 cut(s) 134, 416, 638, 677, 701, 776, 1310
HincII GTYRAC 1 cut(s) 1297
HindII GTYRAC 1 cut(s) 1297
HinfI GANTC 7 cut(s) 18, 175, 612, 924, 1034, 1415, 1553
HpaII CCGG 2 cut(s) 449, 602
HphI GGTGA 2 cut(s) 758, 1053
Hpy166II GTNNAC 4 cut(s) 107, 471, 1297, 1397
Hpy188I TCNGA 3 cut(s) 189, 199, 376
Hpy188III TCNNGA 5 cut(s) 438, 489, 551, 1255, 1365
Hpy8I GTNNAC 4 cut(s) 107, 471, 1297, 1397
HpyAV CCTTC 3 cut(s) 17, 659, 982
HpyCH4III ACNGT 1 cut(s) 1195
HpyF10VI GCNNNNNNNGC 8 cut(s) 635, 764, 773, 1073, 1076, 1079, 1082, 1103
HpyF3I CTNAG 6 cut(s) 436, 864, 926, 937, 1311, 1356
Hsp92II CATG 7 cut(s) 134, 416, 638, 677, 701, 776, 1310
Kzo9I GATC 5 cut(s) 616, 691, 748, 1272, 1361
LmnI GCTCC 3 cut(s) 519, 610, 763
Lsp1109I GCAGC 9 cut(s) 121, 613, 821, 1060, 1063, 1079, 1084, 1091, 1094
LweI GCATC 4 cut(s) 432, 895, 1008, 1358
MaeI CTAG 6 cut(s) 489, 752, 762, 869, 915, 1349
MaeIII GTNAC 3 cut(s) 940, 1059, 1090
MalI GATC 5 cut(s) 618, 693, 750, 1274, 1363
MboI GATC 5 cut(s) 616, 691, 748, 1272, 1361
MboII GAAGA 9 cut(s) 284, 287, 335, 338, 524, 537, 676, 686, 1301
MfeI CAATTG 2 cut(s) 52, 1376
MlyI GAGTC 4 cut(s) 184, 918, 1028, 1547
MmeI TCCRAC 1 cut(s) 167
MroXI GAANNNNTTC 1 cut(s) 294
MseI TTAA 3 cut(s) 243, 543, 854
MslI CAYNNNNRTG 2 cut(s) 417, 1176
MspA1I CMGCKG 3 cut(s) 279, 626, 1070
MspI CCGG 2 cut(s) 449, 602
MspR9I CCNGG 2 cut(s) 300, 1270
MunI CAATTG 2 cut(s) 52, 1376
Mva1269I GAATGC 1 cut(s) 1469
MvaI CCWGG 2 cut(s) 300, 1270
MwoI GCNNNNNNNGC 8 cut(s) 635, 764, 773, 1073, 1076, 1079, 1082, 1103
NdeI CATATG 1 cut(s) 1102
NdeII GATC 5 cut(s) 616, 691, 748, 1272, 1361
NlaIII CATG 7 cut(s) 134, 416, 638, 677, 701, 776, 1310
NlaIV GGNNCC 6 cut(s) 191, 464, 606, 728, 759, 1161
NmuCI GTSAC 2 cut(s) 940, 1059
NspI RCATGY 3 cut(s) 134, 416, 638
NspV TTCGAA 1 cut(s) 1473
PaeI GCATGC 1 cut(s) 134
PceI AGGCCT 1 cut(s) 434
PctI GAATGC 1 cut(s) 1469
PdmI GAANNNNTTC 1 cut(s) 294
PfeI GAWTC 3 cut(s) 18, 612, 1415
PflMI CCANNNNNTGG 1 cut(s) 54
PfoI TCCNGGA 2 cut(s) 298, 1268
PleI GAGTC 4 cut(s) 183, 918, 1028, 1547
PpsI GAGTC 4 cut(s) 183, 918, 1028, 1547
PpuMI RGGWCCY 1 cut(s) 1160
Psp5II RGGWCCY 1 cut(s) 1160
Psp6I CCWGG 2 cut(s) 298, 1268
PspFI CCCAGC 1 cut(s) 1538
PspGI CCWGG 2 cut(s) 298, 1268
PspN4I GGNNCC 6 cut(s) 191, 464, 606, 728, 759, 1161
PspPI GGNCC 5 cut(s) 104, 463, 726, 1160, 1207
PspPPI RGGWCCY 1 cut(s) 1160
PstI CTGCAG 2 cut(s) 631, 1081
PstNI CAGNNNCTG 3 cut(s) 933, 1073, 1088
PvuII CAGCTG 1 cut(s) 626
RsaI GTAC 2 cut(s) 633, 702
RsaNI GTAC 2 cut(s) 632, 701
RseI CAYNNNNRTG 2 cut(s) 417, 1176
SaqAI TTAA 3 cut(s) 243, 543, 854
Sau3AI GATC 5 cut(s) 616, 691, 748, 1272, 1361
Sau96I GGNCC 5 cut(s) 104, 463, 726, 1160, 1207
SchI GAGTC 4 cut(s) 184, 918, 1028, 1547
ScrFI CCNGG 2 cut(s) 300, 1270
SfaNI GCATC 4 cut(s) 432, 895, 1008, 1358
SfcI CTRYAG 4 cut(s) 138, 627, 945, 1077
SfuI TTCGAA 1 cut(s) 1473
SinI GGWCC 3 cut(s) 104, 726, 1160
SmiMI CAYNNNNRTG 2 cut(s) 417, 1176
SpeI ACTAGT 1 cut(s) 1348
SphI GCATGC 1 cut(s) 134
SseBI AGGCCT 1 cut(s) 434
SsiI CCGC 4 cut(s) 279, 813, 1070, 1528
SspI AATATT 1 cut(s) 1285
SspMI CTAG 6 cut(s) 489, 752, 762, 869, 915, 1349
StuI AGGCCT 1 cut(s) 434
StyD4I CCNGG 2 cut(s) 298, 1268
StyI CCWWGG 4 cut(s) 761, 1155, 1320, 1339
TaaI ACNGT 1 cut(s) 1195
TaqI TCGA 5 cut(s) 21, 399, 429, 615, 1473
TatI WGTACW 2 cut(s) 631, 700
TauI GCSGC 2 cut(s) 282, 1073
TfiI GAWTC 3 cut(s) 18, 612, 1415
Tru1I TTAA 3 cut(s) 243, 543, 854
Tru9I TTAA 3 cut(s) 243, 543, 854
TscAI CASTG 6 cut(s) 86, 483, 895, 934, 945, 1399
TseFI GTSAC 2 cut(s) 940, 1059
TseI GCWGC 9 cut(s) 134, 626, 834, 1067, 1073, 1076, 1079, 1082, 1097
Tsp45I GTSAC 2 cut(s) 940, 1059
TspDTI ATGAA 5 cut(s) 165, 385, 686, 807, 1194
TspRI CASTG 6 cut(s) 86, 483, 895, 934, 945, 1399
Van91I CCANNNNNTGG 1 cut(s) 54
VpaK11BI GGWCC 3 cut(s) 104, 726, 1160
XagI CCTNNNNNAGG 1 cut(s) 1214
XapI RAATTY 7 cut(s) 223, 266, 303, 358, 738, 1123, 1264
XbaI TCTAGA 1 cut(s) 488
XceI RCATGY 3 cut(s) 134, 416, 638
XmaJI CCTAGG 1 cut(s) 761
XmnI GAANNNNTTC 1 cut(s) 294
XspI CTAG 6 cut(s) 489, 752, 762, 869, 915, 1349
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.