RchiOBHm_Chr1g0335251

acyl-CoA-binding domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0005156
Physical Location & Seq
Reverse (-)
27432099 .. 27440202
8104 bp
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UTR
Exon/CDS
Intron
PRQ56390

Sequence Viewer

Length: 1551 bp
ATGGGAACACAAGAGATAAAGAAAGATGCGGATGCCAGCAATTGGCAATCTCATTTGGCATATGATCAGTGGGTAGCACTTCCTGTATCTGGTTCACGACCACCAGCTCGCTACAAGCATGCTGCTGTAGTAGTTGATGAAAACTTATATATTGTCGGCGGGAGTCGTAATGGGCGGTATCTATCTGATGTTCAGGTCTTTGACTTTAGAAATTTGTTATGGTCAACTATAAAACTGAATGCAAATTCCAATAATTTTGAAGATAGTGGTTTACAGGAAGTTGTTCCACCCACTTCTTCTCACAATCTGGTGAAGTGGGGAAACAAACTTCTTCTGCTTGGTGGGAATTCAAAGAAATTGTCTGATAAAATAATAGTGTGGTTCATCGATTTGGAAACACACCTCTGTGGCGTCATGGAGACTTCAGGAAAAGTTCCGGCAGCTCGTGCAGGGCAGTCAACTACACTGGTTGGCTCTAGATTGATAATGTTCGGTGGAGAAGACAGGAGCAGGAAATTGTTGAACGATGTCAATGTTCTTGATTTAGAGACTATGACTTGGGATGTGATGGAGACATCGCAGACTCCTCCAGCTCCCAGATTTGATCACACAGCAGCAGTACATGCAGAACGTTACCTTCTAATTTTTGGTGGCTGTTCACATGCTAGTTTCTTCAATGATCTTCATGTTCTGGACTTGCAAGCTATGGAGTGGTCCCAGCCACAAATTCAGGGTGATCTTGTAACTCCTAGGGCAGGTCATGCGGGTATAACTATTGATGAAAACTGGTATATAGTTGGCGGTGGAGATAACAAAAATGGTTGCCCAGAAACCCTGGCCTTAAATATGTCTAAGCTAGTTTGGTCAGTGTCAACAATTGTAAAGCAAAGGGATCCACTTGCCAGTGAGGGTCTCACTGTCTGCTCCGTGAATATTGATGGAAAGAAGCATTTGGTTGCCTTTGGTGGCTATAATGGGAACTATAGCAACGAGGTTTTTGTTATGAGACCCAAACCAAATGACTCTTCACAACGAAAGATATTCCAGTCACCAGCGGCTGCTGCAGCAGCAGCTTCTGTCACTGCTGCCTATGCCTTGACTAAATCAGAAAAATTGAATTTCACAACAGTACAGTCGAACTCCAAGGGAGCTGAAAATCATCGTTCTGAACAAGATTTCACAATTGACATAGAAGCCCTGAGAGAAGAGAAAGGTGTTCTGGAATTGTCTCTTTCCGAAGTCAGGAAAGAAAATGCTAGGGTCAGCCAGGAGATAGATGAAGTCAACAATACTCACAGTGAGTTGTCCAAGGAACTGCAGTCAGTCCAAGGGCAACTTGTAGCTGAGAGATCAAGATGCTTTAAATTGGAGGCTCAAATCAGTGAACTACAGAAGATACTAGAATCAGTGCAGTCCATTGAGGATGAGGTACAGGCACTTCGCGGACAGAAATCTGCAATGGAACGGGATATGGAGCTTGCTTCATCTGTTCAGAGACAAGGTTCTGGTGGTGTCTGGAGATGGATTGCTGGTAGTGGTGGCAACGCATAA

Protein Analysis

516

Amino Acids

56.75

Weight (kDa)

5.64

Isoelectric Point (pI)

40.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_ATRN-LZTR1 PF24981 8 - 155 9.9e-13 Attractin/LZTR1 beta-propeller
Kelch_KLHDC2_KLHL20_DRC7 PF24681 18 - 80 1.2e-12 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 23 - 81 1.2e-08 FBX42, beta-propeller domain
Kelch_1 PF01344 36 - 77 1.6e-09 Kelch motif
Beta-prop_ATRN-LZTR1 PF24981 83 - 221 7.3e-14 Attractin/LZTR1 beta-propeller
Beta-prop_FBX42 PF13415 92 - 221 5.8e-26 FBX42, beta-propeller domain
Kelch_HCF PF13854 96 - 268 1.7e-17 Host cell factor, Kelch-repeats domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 100 - 323 1.1e-53 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 162 - 240 3.3e-13 Attractin/LZTR1 beta-propeller
Beta-prop_TYW4 PF13418 197 - 374 1.6e-06 tRNA wybutosine-synthesizing protein 4, beta-propeller
Beta-prop_FBX42 PF13415 200 - 356 9.6e-21 FBX42, beta-propeller domain
Beta-prop_ATRN-LZTR1 PF24981 225 - 347 1.4e-09 Attractin/LZTR1 beta-propeller
ACBP4_C PF24922 395 - 495 2.3e-37 Acyl-CoA-binding domain-containing protein 4-6, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 746
AccB7I CCANNNNNTGG 1 cut(s) 42
AccII CGCG 1 cut(s) 1443
AciI CCGC 7 cut(s) 29, 159, 175, 764, 801, 1055, 1443
AclI AACGTT 1 cut(s) 631
AclWI GGATC 2 cut(s) 887, 900
AcsI RAATTY 5 cut(s) 211, 244, 346, 726, 1117
AcuI CTGAAG 1 cut(s) 408
AcyI GRCGYC 1 cut(s) 411
AfaI GTAC 3 cut(s) 621, 1131, 1431
AfiI CCNNNNNNNGG 4 cut(s) 42, 89, 755, 1242
AgsI TTSAA 5 cut(s) 260, 351, 523, 676, 1117
AjnI CCWGG 2 cut(s) 834, 1266
AjuI GAANNNNNNNTTGG 2 cut(s) 1009, 1041
AleI CACNNNNGTG 1 cut(s) 405
AluBI AGCT 9 cut(s) 107, 443, 593, 704, 856, 1073, 1151, 1343, 1477
AluI AGCT 9 cut(s) 107, 443, 593, 704, 856, 1073, 1151, 1343, 1477
Alw26I GTCTC 7 cut(s) 413, 542, 566, 917, 1000, 1233, 1489
AlwI GGATC 2 cut(s) 887, 900
AlwNI CAGNNNCTG 2 cut(s) 1058, 1076
AoxI GGCC 1 cut(s) 837
ApeKI GCWGC 9 cut(s) 122, 440, 614, 1058, 1061, 1064, 1067, 1070, 1085
ApoI RAATTY 5 cut(s) 211, 244, 346, 726, 1117
ArsI GACNNNNNNTTYG 2 cut(s) 344, 376
Asp700I GAANNNNTTC 1 cut(s) 282
AspA2I CCTAGG 1 cut(s) 749
AspS9I GGNCC 1 cut(s) 714
AsuHPI GGTGA 3 cut(s) 322, 746, 1041
AvaII GGWCC 1 cut(s) 714
AvrII CCTAGG 1 cut(s) 749
BamHI GGATCC 1 cut(s) 892
BarI GAAGNNNNNNTAC 2 cut(s) 1422, 1454
BauI CACGAG 1 cut(s) 444
BbsI GAAGAC 1 cut(s) 507
BbvI GCAGC 9 cut(s) 109, 452, 626, 1045, 1048, 1072, 1076, 1079, 1082
BccI CCATC 3 cut(s) 562, 932, 1515
BciT130I CCWGG 2 cut(s) 836, 1268
BclI TGATCA 2 cut(s) 64, 604
BcoDI GTCTC 7 cut(s) 413, 542, 566, 917, 1000, 1233, 1489
BfaI CTAG 6 cut(s) 477, 666, 750, 857, 1257, 1400
BfmI CTRYAG 5 cut(s) 126, 982, 1062, 1316, 1388
BfuAI ACCTGC 1 cut(s) 746
BlnI CCTAGG 1 cut(s) 749
Bme1390I CCNGG 2 cut(s) 836, 1268
Bme18I GGWCC 1 cut(s) 714
BmgT120I GGNCC 1 cut(s) 714
BmiI GGNNCC 2 cut(s) 716, 894
BmrFI CCNGG 2 cut(s) 836, 1268
BmsI GCATC 3 cut(s) 16, 22, 1346
BpiI GAAGAC 1 cut(s) 507
BpmI CTGGAG 2 cut(s) 573, 1537
Bsa29I ATCGAT 1 cut(s) 387
BsaHI GRCGYC 1 cut(s) 411
BsaI GGTCTC 2 cut(s) 917, 1000
BsaJI CCNNGG 5 cut(s) 749, 834, 1143, 1308, 1327
Bsc4I CCNNNNNNNGG 4 cut(s) 42, 89, 755, 1242
Bse1I ACTGG 4 cut(s) 471, 791, 903, 1045
Bse3DI GCAATG 1 cut(s) 1464
BseBI CCWGG 2 cut(s) 836, 1268
BseCI ATCGAT 1 cut(s) 387
BseDI CCNNGG 5 cut(s) 749, 834, 1143, 1308, 1327
BseGI GGATG 3 cut(s) 37, 568, 1429
BseLI CCNNNNNNNGG 4 cut(s) 42, 89, 755, 1242
BseMI GCAATG 1 cut(s) 1464
BseMII CTCAG 2 cut(s) 1190, 1335
BseNI ACTGG 4 cut(s) 471, 791, 903, 1045
BseRI GAGGAG 1 cut(s) 576
BseXI GCAGC 9 cut(s) 109, 452, 626, 1045, 1048, 1072, 1076, 1079, 1082
BseYI CCCAGC 1 cut(s) 717
BsgI GTGCAG 2 cut(s) 468, 1430
Bsh1236I CGCG 1 cut(s) 1443
BshFI GGCC 1 cut(s) 839
BshVI ATCGAT 1 cut(s) 387
BsiSI CCGG 1 cut(s) 437
BslFI GGGAC 1 cut(s) 700
BslI CCNNNNNNNGG 4 cut(s) 42, 89, 755, 1242
BsmAI GTCTC 7 cut(s) 413, 542, 566, 917, 1000, 1233, 1489
BsmFI GGGAC 1 cut(s) 700
BsmI GAATGC 1 cut(s) 244
BsnI GGCC 1 cut(s) 839
Bso31I GGTCTC 2 cut(s) 917, 1000
Bsp143I GATC 6 cut(s) 64, 604, 679, 736, 892, 1349
BspACI CCGC 7 cut(s) 29, 159, 175, 764, 801, 1055, 1443
BspANI GGCC 1 cut(s) 839
BspCNI CTCAG 2 cut(s) 1191, 1336
BspDI ATCGAT 1 cut(s) 387
BspFNI CGCG 1 cut(s) 1443
BspLI GGNNCC 2 cut(s) 716, 894
BspMAI CTGCAG 2 cut(s) 1066, 1320
BspMI ACCTGC 1 cut(s) 746
BspPI GGATC 2 cut(s) 887, 900
BspTNI GGTCTC 2 cut(s) 917, 1000
BsrDI GCAATG 1 cut(s) 1464
BsrI ACTGG 4 cut(s) 471, 791, 903, 1045
BssECI CCNNGG 5 cut(s) 749, 834, 1143, 1308, 1327
BssMI GATC 6 cut(s) 64, 604, 679, 736, 892, 1349
BssNI GRCGYC 1 cut(s) 411
BssSI CACGAG 1 cut(s) 444
BssT1I CCWWGG 4 cut(s) 749, 1143, 1308, 1327
Bst2BI CACGAG 1 cut(s) 444
Bst2UI CCWGG 2 cut(s) 836, 1268
Bst4CI ACNGT 4 cut(s) 919, 1129, 1134, 1298
Bst6I CTCTTC 2 cut(s) 1030, 1200
BstACI GRCGYC 1 cut(s) 411
BstAPI GCANNNNNTGC 3 cut(s) 446, 623, 761
BstC8I GCNNGC 5 cut(s) 37, 109, 120, 702, 1479
BstDEI CTNAG 3 cut(s) 852, 1199, 1344
BstENI CCTNNNNNAGG 1 cut(s) 753
BstF5I GGATG 3 cut(s) 37, 568, 1429
BstFNI CGCG 1 cut(s) 1443
BstKTI GATC 6 cut(s) 67, 607, 682, 739, 895, 1352
BstMAI GTCTC 7 cut(s) 413, 542, 566, 917, 1000, 1233, 1489
BstMBI GATC 6 cut(s) 64, 604, 679, 736, 892, 1349
BstMWI GCNNNNNNNGC 8 cut(s) 446, 623, 761, 1061, 1064, 1067, 1070, 1091
BstNI CCWGG 2 cut(s) 836, 1268
BstNSI RCATGY 3 cut(s) 122, 626, 665
BstSCI CCNGG 2 cut(s) 834, 1266
BstSFI CTRYAG 5 cut(s) 126, 982, 1062, 1316, 1388
BstUI CGCG 1 cut(s) 1443
BstV1I GCAGC 9 cut(s) 109, 452, 626, 1045, 1048, 1072, 1076, 1079, 1082
BstV2I GAAGAC 1 cut(s) 507
BstX2I RGATCY 1 cut(s) 892
BstYI RGATCY 1 cut(s) 892
Bsu15I ATCGAT 1 cut(s) 387
BsuRI GGCC 1 cut(s) 839
BsuTUI ATCGAT 1 cut(s) 387
BtgZI GCGATG 1 cut(s) 561
BtsCI GGATG 3 cut(s) 37, 568, 1429
BtsI GCAGTG 1 cut(s) 1080
BtsIMutI CAGTG 9 cut(s) 74, 464, 873, 910, 915, 1080, 1303, 1387, 1413
BveI ACCTGC 1 cut(s) 746
Cac8I GCNNGC 5 cut(s) 37, 109, 120, 702, 1479
CaiI CAGNNNCTG 2 cut(s) 1058, 1076
Cfr13I GGNCC 1 cut(s) 714
ClaI ATCGAT 1 cut(s) 387
CseI GACGC 1 cut(s) 400
Csp6I GTAC 3 cut(s) 620, 1130, 1430
CviAII CATG 6 cut(s) 119, 415, 623, 662, 686, 761
CviQI GTAC 3 cut(s) 620, 1130, 1430
DdeI CTNAG 3 cut(s) 852, 1199, 1344
DpnI GATC 6 cut(s) 66, 606, 681, 738, 894, 1351
DpnII GATC 6 cut(s) 64, 604, 679, 736, 892, 1349
DraI TTTAAA 1 cut(s) 1363
Eam1104I CTCTTC 2 cut(s) 1030, 1200
EarI CTCTTC 2 cut(s) 1030, 1200
Eco130I CCWWGG 4 cut(s) 749, 1143, 1308, 1327
Eco31I GGTCTC 2 cut(s) 917, 1000
Eco47I GGWCC 1 cut(s) 714
Eco57I CTGAAG 1 cut(s) 408
EcoNI CCTNNNNNAGG 1 cut(s) 753
EcoRI GAATTC 1 cut(s) 346
EcoRII CCWGG 2 cut(s) 834, 1266
EcoT14I CCWWGG 4 cut(s) 749, 1143, 1308, 1327
ErhI CCWWGG 4 cut(s) 749, 1143, 1308, 1327
FaeI CATG 6 cut(s) 122, 418, 626, 665, 689, 764
FalI AAGNNNNNCTT 2 cut(s) 1320, 1352
FaqI GGGAC 1 cut(s) 700
FatI CATG 6 cut(s) 118, 414, 622, 661, 685, 760
FauI CCCGC 2 cut(s) 152, 757
FauNDI CATATG 1 cut(s) 61
FbaI TGATCA 2 cut(s) 64, 604
FokI GGATG 3 cut(s) 44, 575, 1436
FspBI CTAG 6 cut(s) 477, 666, 750, 857, 1257, 1400
GsaI CCCAGC 1 cut(s) 721
GsuI CTGGAG 2 cut(s) 573, 1537
HaeIII GGCC 1 cut(s) 839
HapII CCGG 1 cut(s) 437
HgaI GACGC 1 cut(s) 400
Hin1I GRCGYC 1 cut(s) 411
Hin1II CATG 6 cut(s) 122, 418, 626, 665, 689, 764
HincII GTYRAC 4 cut(s) 225, 459, 873, 1285
HindII GTYRAC 4 cut(s) 225, 459, 873, 1285
HinfI GANTC 4 cut(s) 163, 583, 1022, 1403
HpaII CCGG 1 cut(s) 437
HphI GGTGA 3 cut(s) 322, 746, 1041
Hpy166II GTNNAC 8 cut(s) 95, 225, 272, 459, 659, 873, 1285, 1385
Hpy188I TCNGA 6 cut(s) 187, 364, 1108, 1168, 1237, 1494
Hpy188III TCNNGA 9 cut(s) 96, 426, 477, 539, 692, 1220, 1243, 1353, 1516
Hpy8I GTNNAC 8 cut(s) 95, 225, 272, 459, 659, 873, 1285, 1385
HpyAV CCTTC 1 cut(s) 647
HpyCH4III ACNGT 4 cut(s) 919, 1129, 1134, 1298
HpyCH4IV ACGT 1 cut(s) 631
HpyCH4V TGCA 8 cut(s) 242, 449, 626, 700, 1064, 1318, 1411, 1457
HpyF10VI GCNNNNNNNGC 8 cut(s) 446, 623, 761, 1061, 1064, 1067, 1070, 1091
HpyF3I CTNAG 3 cut(s) 852, 1199, 1344
HpySE526I ACGT 1 cut(s) 631
Hsp92I GRCGYC 1 cut(s) 411
Hsp92II CATG 6 cut(s) 122, 418, 626, 665, 689, 764
Ksp22I TGATCA 2 cut(s) 64, 604
Kzo9I GATC 6 cut(s) 64, 604, 679, 736, 892, 1349
LmnI GCTCC 5 cut(s) 507, 598, 929, 1148, 1474
Lsp1109I GCAGC 9 cut(s) 109, 452, 626, 1045, 1048, 1072, 1076, 1079, 1082
LweI GCATC 3 cut(s) 16, 22, 1346
MaeI CTAG 6 cut(s) 477, 666, 750, 857, 1257, 1400
MaeII ACGT 1 cut(s) 631
MaeIII GTNAC 4 cut(s) 632, 742, 1047, 1078
MalI GATC 6 cut(s) 66, 606, 681, 738, 894, 1351
MboI GATC 6 cut(s) 64, 604, 679, 736, 892, 1349
MboII GAAGA 9 cut(s) 272, 288, 323, 512, 664, 674, 1017, 1217, 1405
MfeI CAATTG 3 cut(s) 40, 876, 1182
MflI RGATCY 1 cut(s) 892
MlyI GAGTC 3 cut(s) 172, 577, 1016
MnlI CCTC 7 cut(s) 413, 597, 901, 985, 1363, 1414, 1420
MroXI GAANNNNTTC 1 cut(s) 282
MseI TTAA 2 cut(s) 842, 1362
MslI CAYNNNNRTG 1 cut(s) 405
MspA1I CMGCKG 1 cut(s) 1055
MspI CCGG 1 cut(s) 437
MspR9I CCNGG 2 cut(s) 836, 1268
MunI CAATTG 3 cut(s) 40, 876, 1182
Mva1269I GAATGC 1 cut(s) 244
MvaI CCWGG 2 cut(s) 836, 1268
MvnI CGCG 1 cut(s) 1443
MwoI GCNNNNNNNGC 8 cut(s) 446, 623, 761, 1061, 1064, 1067, 1070, 1091
NdeI CATATG 1 cut(s) 61
NdeII GATC 6 cut(s) 64, 604, 679, 736, 892, 1349
NlaIII CATG 6 cut(s) 122, 418, 626, 665, 689, 764
NlaIV GGNNCC 2 cut(s) 716, 894
NmuCI GTSAC 2 cut(s) 1047, 1078
NspI RCATGY 3 cut(s) 122, 626, 665
OliI CACNNNNGTG 1 cut(s) 405
PaeI GCATGC 1 cut(s) 122
PctI GAATGC 1 cut(s) 244
PdmI GAANNNNTTC 1 cut(s) 282
PfeI GAWTC 1 cut(s) 1403
PflMI CCANNNNNTGG 1 cut(s) 42
PleI GAGTC 3 cut(s) 171, 577, 1016
PpsI GAGTC 3 cut(s) 171, 577, 1016
Psp1406I AACGTT 1 cut(s) 631
Psp6I CCWGG 2 cut(s) 834, 1266
PspFI CCCAGC 1 cut(s) 717
PspGI CCWGG 2 cut(s) 834, 1266
PspN4I GGNNCC 2 cut(s) 716, 894
PspPI GGNCC 1 cut(s) 714
PstI CTGCAG 2 cut(s) 1066, 1320
PstNI CAGNNNCTG 2 cut(s) 1058, 1076
PsuI RGATCY 1 cut(s) 892
RsaI GTAC 3 cut(s) 621, 1131, 1431
RsaNI GTAC 3 cut(s) 620, 1130, 1430
RseI CAYNNNNRTG 1 cut(s) 405
SaqAI TTAA 2 cut(s) 842, 1362
Sau3AI GATC 6 cut(s) 64, 604, 679, 736, 892, 1349
Sau96I GGNCC 1 cut(s) 714
SchI GAGTC 3 cut(s) 172, 577, 1016
ScrFI CCNGG 2 cut(s) 836, 1268
SfaNI GCATC 3 cut(s) 16, 22, 1346
SfcI CTRYAG 5 cut(s) 126, 982, 1062, 1316, 1388
SinI GGWCC 1 cut(s) 714
SmiMI CAYNNNNRTG 1 cut(s) 405
SphI GCATGC 1 cut(s) 122
SsiI CCGC 7 cut(s) 29, 159, 175, 764, 801, 1055, 1443
SspI AATATT 1 cut(s) 934
SspMI CTAG 6 cut(s) 477, 666, 750, 857, 1257, 1400
StyD4I CCNGG 2 cut(s) 834, 1266
StyI CCWWGG 4 cut(s) 749, 1143, 1308, 1327
TaaI ACNGT 4 cut(s) 919, 1129, 1134, 1298
TaiI ACGT 1 cut(s) 634
TaqI TCGA 2 cut(s) 387, 1136
TatI WGTACW 2 cut(s) 619, 1129
TauI GCSGC 1 cut(s) 1058
TfiI GAWTC 1 cut(s) 1403
Tru1I TTAA 2 cut(s) 842, 1362
Tru9I TTAA 2 cut(s) 842, 1362
TscAI CASTG 9 cut(s) 74, 471, 873, 910, 922, 1087, 1303, 1387, 1413
TseFI GTSAC 2 cut(s) 1047, 1078
TseI GCWGC 9 cut(s) 122, 440, 614, 1058, 1061, 1064, 1067, 1070, 1085
Tsp45I GTSAC 2 cut(s) 1047, 1078
TspDTI ATGAA 6 cut(s) 153, 373, 674, 795, 1293, 1473
TspGWI ACGGA 1 cut(s) 916
TspRI CASTG 9 cut(s) 74, 471, 873, 910, 922, 1087, 1303, 1387, 1413
Van91I CCANNNNNTGG 1 cut(s) 42
VpaK11BI GGWCC 1 cut(s) 714
XagI CCTNNNNNAGG 1 cut(s) 753
XapI RAATTY 5 cut(s) 211, 244, 346, 726, 1117
XbaI TCTAGA 1 cut(s) 476
XceI RCATGY 3 cut(s) 122, 626, 665
XmaJI CCTAGG 1 cut(s) 749
XmnI GAANNNNTTC 1 cut(s) 282
XspI CTAG 6 cut(s) 477, 666, 750, 857, 1257, 1400
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.