Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0005156

Physical Location & Seq

Genomic Coordinates Reverse (-)

7023192 .. 7029233

Gene Structure 6042 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1074000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1623 bp

ATGCGAAATGGGCAATTGCAATTTTGTCACAGGATTGAGGACTCTGTTTATCCTGAAATGTTGGTGGAAACAGCTTCTATGGGAAACAAAGGGATTGTGAAAAATGTTGATACCAGCAGTTGGCAAACAGATTTGGCCTGTGACCAGTGGATAGCACTCCCTGTTTCTGGTCCACGACCACCAGCTCGCTATAAGCATGCTGCTGTAGTAGTGGATGAAAAATTATACATTGTTGGTGGGAGTCGTAATGGTCGGCACCTATCTGATGTTCAGGTCTTTGACTTTAGAAATTTGTTGTGGTCTAAAATTAAACTGAAACCCAATTCCAATAAATTTGAAGACAGCGGCTTACAGGAAGTTCTTCCAGGAATTTCTGCTCACAATATGGTTAGGTGGGGAGACAAACTTCTTCTTCTTGGTGGGAATTCGAAGAAATCGTCTGATAGTATAATAGTTTGGTTCATCGATTTGGAAACGCACCACTGTGGCATCATTGAGACCTCAGGAAATGTACCGGTAGCTCGTGGGGGGCAGTCCATGACATTGGTCGGTTCTAGACTGATAATGTTCGGTGGAGAAGACAGGACCAGAAGATTGTTGAATGATGTTAATGTTCTTGATCTAGAGACAATGACTTGGGATGTGATGAAAGTAACGCAGACACCTCCAGCTCCTAGATTCGATCACGCAGCTGCAGTAAATGCTGAACGCTACCTTCTAATTTTTGGTGGTTGTTCTCATGCTACTTTCTTCAATGATCTTCATGTACTTGACTTGGAAGCAATGGAGTGGTCCCAACCGCAAATTCAGGGTGATCTTGTTGCTCCTAGGGCAGGTCATGCTGGTATAACCATTGATGAAAACTGGTATATAGTTGGTGGTGGAGATAACAAAAATGGTTGCCCTGAAACTCTGGTGTTAAATATGTCTAAGCTAGTGTGGTCAGTCTCAACCAGTGTTAAGCAAAGGGATGCACTCGCTAGTGAGGGACTCACTGTCTGCTCGGCGACCATAGATGGAAAGAAGCATTTGGTTGCCTTTGGTGGGTATAATGGGAACTATAGCAATGAGGTTTTTGTAATGATGCCCAAACCGAGGGACTCATCACAACCCAAGATATTCCAGTCACCAGCAGCAGCTGCAGCGGCAGCTTCTGTTACTGCTGCATATGCCTTATCTAAAATAGAAAATTTCGATTCCAAAACTGCAGAGCTGAACTCCAAGGGACCTGAAAACCATCATTATGAACAAGATTTAACAGTTGAGATAGAGGCCCTTTCAGAGGAGAAAAGTGTGCTGGAGTTGTCGCTTGCGGAAGTCAGGGCAGAAAATTCCAGGATCGGGCAGAATATTGAAGAAGTCAACAATACTCATGCTGAGTTGTCCAAGGAACTTCTTTCAGTCCAAGGACAACTAGTAGCTGAGAGATCAAGATGCTTTAAATTGGAGGCTCAAATCAGTGAACTGCAAAAGAAATTAGAATCGGTGCAGTCCATTGAGAATGAGGTGCAAGCACTTCGGGGTCAAAAATCTGCTTTGGAACGGGATGAGCTCACCTCTTCTGCTCAGAGACAAGGTTCCGGTGGGGTTTGGCGGTGGATTGCTGGGGATGGTGGTGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000062 GO:0000166 GO:0001101 GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006810 GO:0006869 GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009719 GO:0009723 GO:0009725 GO:0009753 GO:0010033 GO:0010876 GO:0017076 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0033036 GO:0033218 GO:0036094 GO:0042221 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0048037 GO:0050662 GO:0050896 GO:0051179 GO:0051234 GO:0071702 GO:0097159 GO:0097367 GO:1901265 GO:1901363 GO:1901567 GO:1901681 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kelch_1 Kelch_2 Kelch_3 Kelch_4 Kelch_5 Kelch_6

Protein Analysis

541

Amino Acids

59.36

Weight (kDa)

5.49

Isoelectric Point (pI)

42.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Kelch_HCF	PF13854	25 - 244	2.9e-14	Host cell factor, Kelch-repeats domain
Beta-prop_ATRN-LZTR1	PF24981	39 - 181	2.2e-14	Attractin/LZTR1 beta-propeller
Kelch_KLHDC2_KLHL20_DRC7	PF24681	41 - 106	1.8e-13	KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42	PF13415	41 - 107	9.4e-10	FBX42, beta-propeller domain
Kelch_1	PF01344	62 - 103	2.1e-10	Kelch motif
Kelch_HCF	PF13854	120 - 294	2.2e-16	Host cell factor, Kelch-repeats domain
Beta-prop_FBX42	PF13415	124 - 248	1.4e-25	FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7	PF24681	127 - 319	1.6e-51	KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_ATRN-LZTR1	PF24981	138 - 282	7.1e-19	Attractin/LZTR1 beta-propeller
Kelch_2	PF07646	175 - 217	7.8e-06	Kelch motif
Beta-prop_FBX42	PF13415	227 - 385	3.7e-20	FBX42, beta-propeller domain
Beta-prop_ATRN-LZTR1	PF24981	240 - 364	2e-10	Attractin/LZTR1 beta-propeller
ACBP4_C	PF24922	421 - 523	8.5e-38	Acyl-CoA-binding domain-containing protein 4-6, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0005156)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G04420 AT5G04420 AT5G04420
fragaria_vesca	FvH4_7g06590 FvH4_7g06590 FvH4_7g06590 FvH4_7g06590 FvH4_7g06590 FvH4_7g06590 FvH4_7g06590 FvH4_7g06590
malus_domestica	MD02G1242500.v1.1 MD07G1074000.v1.1
prunus_persica	Prupe.2G091900_v2.0.a1
pyrus_communis	pycom02g20920 pycom07g05810
rosa_chinensis	RchiOBHm_Chr1g0335251
rosa_laevigata	RLG00000029392
rosa_multiflora	Rmu_sc0002714.1_g000005
rosa_roxburghii	Rroxscaffold_3G00240470 Rroxscaffold_4G00316130 Rroxscaffold_4G00316210
rosa_rugosa	Rorug01G0121200 Rorug01G0121300 Rorug01G0121400
rosa_samantha	Rh1AG142800 Rh1BG111400 Rh1CG134600 Rh1DG148400
rosa_wichuraiana	Rw1G011800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	824
AccB1I	GGYRCC	1 cut(s)	255
AccB7I	CCANNNNNTGG	1 cut(s)	120
AciI	CCGC	5 cut(s)	345, 800, 1145, 1313, 1594
AclWI	GGATC	1 cut(s)	1346
AcsI	RAATTY	7 cut(s)	289, 332, 369, 424, 804, 1189, 1330
AfaI	GTAC	2 cut(s)	513, 768
AfiI	CCNNNNNNNGG	7 cut(s)	120, 167, 833, 1044, 1095, 1282, 1341
AgeI	ACCGGT	1 cut(s)	514
AgsI	TTSAA	4 cut(s)	338, 601, 754, 1355
AhdI	GACNNNNNGTC	1 cut(s)	995
AhlI	ACTAGT	1 cut(s)	1414
AjnI	CCWGG	2 cut(s)	364, 1334
AjuI	GAANNNNNNNTTGG	2 cut(s)	789, 821
AleI	CACNNNNGTG	1 cut(s)	483
Alw21I	GWGCWC	1 cut(s)	1554
Alw26I	GTCTC	5 cut(s)	393, 491, 620, 952, 1564
AlwI	GGATC	1 cut(s)	1346
AlwNI	CAGNNNCTG	2 cut(s)	1139, 1154
AoxI	GGCC	2 cut(s)	135, 1272
ApeKI	GCWGC	9 cut(s)	200, 689, 692, 1133, 1136, 1139, 1142, 1148, 1163
ApoI	RAATTY	7 cut(s)	289, 332, 369, 424, 804, 1189, 1330
ArsI	GACNNNNNNTTYG	2 cut(s)	422, 454
AsiGI	ACCGGT	1 cut(s)	514
Asp700I	GAANNNNTTC	1 cut(s)	360
AspA2I	CCTAGG	1 cut(s)	827
AspS9I	GGNCC	5 cut(s)	170, 585, 792, 1226, 1273
AsuHPI	GGTGA	3 cut(s)	824, 1119, 1546
AsuII	TTCGAA	1 cut(s)	428
AvaII	GGWCC	4 cut(s)	170, 585, 792, 1226
AvrII	CCTAGG	1 cut(s)	827
AxyI	CCTNAGG	1 cut(s)	502
BanI	GGYRCC	1 cut(s)	255
BanII	GRGCYC	1 cut(s)	1554
BauI	CACGAG	1 cut(s)	522
BbsI	GAAGAC	2 cut(s)	345, 585
Bbv12I	GWGCWC	1 cut(s)	1554
BbvI	GCAGC	9 cut(s)	187, 679, 701, 1126, 1145, 1148, 1150, 1154, 1160
BccI	CCATC	3 cut(s)	1010, 1245, 1604
BcgI	CGANNNNNNTGC	2 cut(s)	1499, 1533
BciT130I	CCWGG	2 cut(s)	366, 1336
BcoDI	GTCTC	5 cut(s)	393, 491, 620, 952, 1564
BcuI	ACTAGT	1 cut(s)	1414
BfaI	CTAG	7 cut(s)	555, 623, 675, 828, 935, 981, 1415
BfmI	CTRYAG	5 cut(s)	204, 693, 1060, 1140, 1206
BfuAI	ACCTGC	1 cut(s)	824
BlnI	CCTAGG	1 cut(s)	827
Bme1390I	CCNGG	2 cut(s)	366, 1336
Bme18I	GGWCC	4 cut(s)	170, 585, 792, 1226
BmeRI	GACNNNNNGTC	1 cut(s)	995
BmgT120I	GGNCC	5 cut(s)	170, 585, 792, 1226, 1273
BmiI	GGNNCC	4 cut(s)	257, 794, 1227, 1579
BmrFI	CCNGG	2 cut(s)	366, 1336
BmsI	GCATC	4 cut(s)	498, 961, 1074, 1424
BoxI	GACNNNNGTC	1 cut(s)	545
BpiI	GAAGAC	2 cut(s)	345, 585
BplI	GAGNNNNNCTC	4 cut(s)	1202, 1234, 1541, 1573
BpmI	CTGGAG	2 cut(s)	651, 1319
Bpu14I	TTCGAA	1 cut(s)	428
Bsa29I	ATCGAT	1 cut(s)	465
BsaI	GGTCTC	1 cut(s)	491
BsaJI	CCNNGG	5 cut(s)	827, 1094, 1221, 1386, 1405
BsaWI	WCCGGW	2 cut(s)	514, 1580
Bsc4I	CCNNNNNNNGG	7 cut(s)	120, 167, 833, 1044, 1095, 1282, 1341
Bse118I	RCCGGY	1 cut(s)	514
Bse1I	ACTGG	4 cut(s)	145, 869, 954, 1123
Bse21I	CCTNAGG	1 cut(s)	502
Bse3DI	GCAATG	2 cut(s)	789, 1072
BseBI	CCWGG	2 cut(s)	366, 1336
BseCI	ATCGAT	1 cut(s)	465
BseDI	CCNNGG	5 cut(s)	827, 1094, 1221, 1386, 1405
BseGI	GGATG	5 cut(s)	220, 646, 976, 1552, 1615
BseLI	CCNNNNNNNGG	7 cut(s)	120, 167, 833, 1044, 1095, 1282, 1341
BseMI	GCAATG	2 cut(s)	789, 1072
BseMII	CTCAG	4 cut(s)	516, 1368, 1413, 1580
BseNI	ACTGG	4 cut(s)	145, 869, 954, 1123
BseRI	GAGGAG	1 cut(s)	1298
BseXI	GCAGC	9 cut(s)	187, 679, 701, 1126, 1145, 1148, 1150, 1154, 1160
BseYI	CCCAGC	1 cut(s)	1604
BsgI	GTGCAG	1 cut(s)	1508
BshFI	GGCC	2 cut(s)	137, 1274
BshNI	GGYRCC	1 cut(s)	255
BshTI	ACCGGT	1 cut(s)	514
BshVI	ATCGAT	1 cut(s)	465
BsiHKAI	GWGCWC	1 cut(s)	1554
BsiSI	CCGG	2 cut(s)	515, 1581
BslFI	GGGAC	4 cut(s)	778, 1002, 1112, 1239
BslI	CCNNNNNNNGG	7 cut(s)	120, 167, 833, 1044, 1095, 1282, 1341
BsmAI	GTCTC	5 cut(s)	393, 491, 620, 952, 1564
BsmFI	GGGAC	4 cut(s)	778, 1002, 1112, 1239
BsnI	GGCC	2 cut(s)	137, 1274
Bso31I	GGTCTC	1 cut(s)	491
Bsp119I	TTCGAA	1 cut(s)	428
Bsp1286I	GDGCHC	1 cut(s)	1554
Bsp143I	GATC	6 cut(s)	619, 682, 757, 814, 1338, 1427
BspACI	CCGC	5 cut(s)	345, 800, 1145, 1313, 1594
BspANI	GGCC	2 cut(s)	137, 1274
BspCNI	CTCAG	4 cut(s)	515, 1369, 1414, 1579
BspDI	ATCGAT	1 cut(s)	465
BspLI	GGNNCC	4 cut(s)	257, 794, 1227, 1579
BspMAI	CTGCAG	3 cut(s)	697, 1144, 1210
BspMI	ACCTGC	1 cut(s)	824
BspPI	GGATC	1 cut(s)	1346
BspT104I	TTCGAA	1 cut(s)	428
BspT107I	GGYRCC	1 cut(s)	255
BspTNI	GGTCTC	1 cut(s)	491
BsrDI	GCAATG	2 cut(s)	789, 1072
BsrFI	RCCGGY	1 cut(s)	514
BsrI	ACTGG	4 cut(s)	145, 869, 954, 1123
BssAI	RCCGGY	1 cut(s)	514
BssECI	CCNNGG	5 cut(s)	827, 1094, 1221, 1386, 1405
BssMI	GATC	6 cut(s)	619, 682, 757, 814, 1338, 1427
BssSI	CACGAG	1 cut(s)	522
BssT1I	CCWWGG	4 cut(s)	827, 1221, 1386, 1405
Bst2BI	CACGAG	1 cut(s)	522
Bst2UI	CCWGG	2 cut(s)	366, 1336
Bst4CI	ACNGT	3 cut(s)	485, 997, 1261
Bst6I	CTCTTC	1 cut(s)	1564
BstAPI	GCANNNNNTGC	3 cut(s)	701, 839, 1139
BstBI	TTCGAA	1 cut(s)	428
BstC8I	GCNNGC	4 cut(s)	187, 198, 1311, 1512
BstDEI	CTNAG	5 cut(s)	502, 930, 1377, 1422, 1566
BstENI	CCTNNNNNAGG	2 cut(s)	831, 1280
BstF5I	GGATG	5 cut(s)	220, 646, 976, 1552, 1615
BstKTI	GATC	6 cut(s)	622, 685, 760, 817, 1341, 1430
BstMAI	GTCTC	5 cut(s)	393, 491, 620, 952, 1564
BstMBI	GATC	6 cut(s)	619, 682, 757, 814, 1338, 1427
BstMWI	GCNNNNNNNGC	9 cut(s)	10, 701, 830, 839, 1139, 1142, 1145, 1148, 1169
BstNI	CCWGG	2 cut(s)	366, 1336
BstNSI	RCATGY	1 cut(s)	200
BstPAI	GACNNNNGTC	1 cut(s)	545
BstSCI	CCNGG	2 cut(s)	364, 1334
BstSFI	CTRYAG	5 cut(s)	204, 693, 1060, 1140, 1206
BstV1I	GCAGC	9 cut(s)	187, 679, 701, 1126, 1145, 1148, 1150, 1154, 1160
BstV2I	GAAGAC	2 cut(s)	345, 585
BstXI	CCANNNNNNTGG	1 cut(s)	544
Bsu15I	ATCGAT	1 cut(s)	465
Bsu36I	CCTNAGG	1 cut(s)	502
BsuRI	GGCC	2 cut(s)	137, 1274
BsuTUI	ATCGAT	1 cut(s)	465
BtsCI	GGATG	5 cut(s)	220, 646, 976, 1552, 1615
BtsIMutI	CAGTG	5 cut(s)	152, 481, 961, 993, 1465
BveI	ACCTGC	1 cut(s)	824
Cac8I	GCNNGC	4 cut(s)	187, 198, 1311, 1512
CaiI	CAGNNNCTG	2 cut(s)	1139, 1154
Cfr10I	RCCGGY	1 cut(s)	514
Cfr13I	GGNCC	5 cut(s)	170, 585, 792, 1226, 1273
ClaI	ATCGAT	1 cut(s)	465
Csp6I	GTAC	2 cut(s)	512, 767
CspAI	ACCGGT	1 cut(s)	514
CviAII	CATG	6 cut(s)	197, 538, 740, 764, 839, 1373
CviQI	GTAC	2 cut(s)	512, 767
DdeI	CTNAG	5 cut(s)	502, 930, 1377, 1422, 1566
DpnI	GATC	6 cut(s)	621, 684, 759, 816, 1340, 1429
DpnII	GATC	6 cut(s)	619, 682, 757, 814, 1338, 1427
DraI	TTTAAA	1 cut(s)	1441
DriI	GACNNNNNGTC	1 cut(s)	995
Eam1104I	CTCTTC	1 cut(s)	1564
Eam1105I	GACNNNNNGTC	1 cut(s)	995
EarI	CTCTTC	1 cut(s)	1564
Ecl136II	GAGCTC	1 cut(s)	1552
Eco130I	CCWWGG	4 cut(s)	827, 1221, 1386, 1405
Eco24I	GRGCYC	1 cut(s)	1554
Eco31I	GGTCTC	1 cut(s)	491
Eco47I	GGWCC	4 cut(s)	170, 585, 792, 1226
Eco53kI	GAGCTC	1 cut(s)	1552
Eco81I	CCTNAGG	1 cut(s)	502
EcoICRI	GAGCTC	1 cut(s)	1552
EcoNI	CCTNNNNNAGG	2 cut(s)	831, 1280
EcoO109I	RGGNCCY	2 cut(s)	1226, 1273
EcoRI	GAATTC	1 cut(s)	424
EcoRII	CCWGG	2 cut(s)	364, 1334
EcoT14I	CCWWGG	4 cut(s)	827, 1221, 1386, 1405
EcoT38I	GRGCYC	1 cut(s)	1554
ErhI	CCWWGG	4 cut(s)	827, 1221, 1386, 1405
FaeI	CATG	6 cut(s)	200, 541, 743, 767, 842, 1376
FaqI	GGGAC	4 cut(s)	778, 1002, 1112, 1239
FatI	CATG	6 cut(s)	196, 537, 739, 763, 838, 1372
FauNDI	CATATG	1 cut(s)	1168
FokI	GGATG	4 cut(s)	227, 653, 983, 1559
FriOI	GRGCYC	1 cut(s)	1554
FspBI	CTAG	7 cut(s)	555, 623, 675, 828, 935, 981, 1415
GsaI	CCCAGC	1 cut(s)	1608
GsuI	CTGGAG	2 cut(s)	651, 1319
HaeIII	GGCC	2 cut(s)	137, 1274
HapII	CCGG	2 cut(s)	515, 1581
Hin1II	CATG	6 cut(s)	200, 541, 743, 767, 842, 1376
HincII	GTYRAC	1 cut(s)	1363
HindII	GTYRAC	1 cut(s)	1363
HinfI	GANTC	7 cut(s)	41, 241, 678, 990, 1100, 1196, 1481
HpaII	CCGG	2 cut(s)	515, 1581
HphI	GGTGA	3 cut(s)	824, 1119, 1546
Hpy166II	GTNNAC	3 cut(s)	173, 1363, 1463
Hpy188I	TCNGA	4 cut(s)	265, 442, 1282, 1569
Hpy188III	TCNNGA	6 cut(s)	53, 504, 555, 617, 623, 1431
Hpy8I	GTNNAC	3 cut(s)	173, 1363, 1463
HpyAV	CCTTC	1 cut(s)	725
HpyCH4III	ACNGT	3 cut(s)	485, 997, 1261
HpyCH4V	TGCA	9 cut(s)	19, 695, 974, 1142, 1166, 1208, 1468, 1489, 1510
HpyF10VI	GCNNNNNNNGC	9 cut(s)	10, 701, 830, 839, 1139, 1142, 1145, 1148, 1169
HpyF3I	CTNAG	5 cut(s)	502, 930, 1377, 1422, 1566
Hsp92II	CATG	6 cut(s)	200, 541, 743, 767, 842, 1376
Kzo9I	GATC	6 cut(s)	619, 682, 757, 814, 1338, 1427
LmnI	GCTCC	2 cut(s)	676, 829
Lsp1109I	GCAGC	9 cut(s)	187, 679, 701, 1126, 1145, 1148, 1150, 1154, 1160
LweI	GCATC	4 cut(s)	498, 961, 1074, 1424
MaeI	CTAG	7 cut(s)	555, 623, 675, 828, 935, 981, 1415
MaeIII	GTNAC	6 cut(s)	26, 140, 652, 1125, 1156, 1616
MalI	GATC	6 cut(s)	621, 684, 759, 816, 1340, 1429
MboI	GATC	6 cut(s)	619, 682, 757, 814, 1338, 1427
MfeI	CAATTG	1 cut(s)	14
MhlI	GDGCHC	1 cut(s)	1554
MlyI	GAGTC	4 cut(s)	35, 250, 984, 1094
MroXI	GAANNNNTTC	1 cut(s)	360
MseI	TTAA	6 cut(s)	309, 609, 920, 960, 1256, 1440
MslI	CAYNNNNRTG	2 cut(s)	483, 1242
MspA1I	CMGCKG	4 cut(s)	345, 692, 1139, 1145
MspI	CCGG	2 cut(s)	515, 1581
MspR9I	CCNGG	2 cut(s)	366, 1336
MunI	CAATTG	1 cut(s)	14
MvaI	CCWGG	2 cut(s)	366, 1336
MwoI	GCNNNNNNNGC	9 cut(s)	10, 701, 830, 839, 1139, 1142, 1145, 1148, 1169
NdeI	CATATG	1 cut(s)	1168
NdeII	GATC	6 cut(s)	619, 682, 757, 814, 1338, 1427
NlaIII	CATG	6 cut(s)	200, 541, 743, 767, 842, 1376
NlaIV	GGNNCC	4 cut(s)	257, 794, 1227, 1579
NmeAIII	GCCGAG	1 cut(s)	983
NmuCI	GTSAC	4 cut(s)	26, 140, 1125, 1616
NspI	RCATGY	1 cut(s)	200
NspV	TTCGAA	1 cut(s)	428
OliI	CACNNNNGTG	1 cut(s)	483
PaeI	GCATGC	1 cut(s)	200
PcsI	WCGNNNNNNNCGW	1 cut(s)	434
PdmI	GAANNNNTTC	1 cut(s)	360
PfeI	GAWTC	3 cut(s)	678, 1196, 1481
PflMI	CCANNNNNTGG	1 cut(s)	120
PfoI	TCCNGGA	2 cut(s)	364, 1334
PinAI	ACCGGT	1 cut(s)	514
PleI	GAGTC	4 cut(s)	35, 249, 984, 1094
PpsI	GAGTC	4 cut(s)	35, 249, 984, 1094
PpuMI	RGGWCCY	1 cut(s)	1226
PshAI	GACNNNNGTC	1 cut(s)	545
Psp124BI	GAGCTC	1 cut(s)	1554
Psp5II	RGGWCCY	1 cut(s)	1226
Psp6I	CCWGG	2 cut(s)	364, 1334
PspFI	CCCAGC	1 cut(s)	1604
PspGI	CCWGG	2 cut(s)	364, 1334
PspN4I	GGNNCC	4 cut(s)	257, 794, 1227, 1579
PspPI	GGNCC	5 cut(s)	170, 585, 792, 1226, 1273
PspPPI	RGGWCCY	1 cut(s)	1226
PstI	CTGCAG	3 cut(s)	697, 1144, 1210
PstNI	CAGNNNCTG	2 cut(s)	1139, 1154
PvuII	CAGCTG	2 cut(s)	692, 1139
RsaI	GTAC	2 cut(s)	513, 768
RsaNI	GTAC	2 cut(s)	512, 767
RseI	CAYNNNNRTG	2 cut(s)	483, 1242
SacI	GAGCTC	1 cut(s)	1554
SaqAI	TTAA	6 cut(s)	309, 609, 920, 960, 1256, 1440
Sau3AI	GATC	6 cut(s)	619, 682, 757, 814, 1338, 1427
Sau96I	GGNCC	5 cut(s)	170, 585, 792, 1226, 1273
SchI	GAGTC	4 cut(s)	35, 250, 984, 1094
ScrFI	CCNGG	2 cut(s)	366, 1336
SduI	GDGCHC	1 cut(s)	1554
SfaNI	GCATC	4 cut(s)	498, 961, 1074, 1424
SfcI	CTRYAG	5 cut(s)	204, 693, 1060, 1140, 1206
SfuI	TTCGAA	1 cut(s)	428
SinI	GGWCC	4 cut(s)	170, 585, 792, 1226
SmiMI	CAYNNNNRTG	2 cut(s)	483, 1242
SpeI	ACTAGT	1 cut(s)	1414
SphI	GCATGC	1 cut(s)	200
SsiI	CCGC	5 cut(s)	345, 800, 1145, 1313, 1594
SspI	AATATT	1 cut(s)	1351
SspMI	CTAG	7 cut(s)	555, 623, 675, 828, 935, 981, 1415
SstI	GAGCTC	1 cut(s)	1554
StyD4I	CCNGG	2 cut(s)	364, 1334
StyI	CCWWGG	4 cut(s)	827, 1221, 1386, 1405
TaaI	ACNGT	3 cut(s)	485, 997, 1261
TaqI	TCGA	4 cut(s)	428, 465, 681, 1194
TatI	WGTACW	1 cut(s)	766
TauI	GCSGC	2 cut(s)	348, 1148
TfiI	GAWTC	3 cut(s)	678, 1196, 1481
Tru1I	TTAA	6 cut(s)	309, 609, 920, 960, 1256, 1440
Tru9I	TTAA	6 cut(s)	309, 609, 920, 960, 1256, 1440
TscAI	CASTG	5 cut(s)	152, 488, 961, 1000, 1465
TseFI	GTSAC	4 cut(s)	26, 140, 1125, 1616
TseI	GCWGC	9 cut(s)	200, 689, 692, 1133, 1136, 1139, 1142, 1148, 1163
Tsp45I	GTSAC	4 cut(s)	26, 140, 1125, 1616
TspDTI	ATGAA	6 cut(s)	231, 451, 662, 752, 873, 1260
TspRI	CASTG	5 cut(s)	152, 488, 961, 1000, 1465
Van91I	CCANNNNNTGG	1 cut(s)	120
VpaK11BI	GGWCC	4 cut(s)	170, 585, 792, 1226
XagI	CCTNNNNNAGG	2 cut(s)	831, 1280
XapI	RAATTY	7 cut(s)	289, 332, 369, 424, 804, 1189, 1330
XbaI	TCTAGA	2 cut(s)	554, 622
XceI	RCATGY	1 cut(s)	200
XmaJI	CCTAGG	1 cut(s)	827
XmnI	GAANNNNTTC	1 cut(s)	360
XspI	CTAG	7 cut(s)	555, 623, 675, 828, 935, 981, 1415

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.