Rroxscaffold_4G00316210

acyl-CoA-binding domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0005156
Physical Location & Seq
Reverse (-)
40878671 .. 40885809
7139 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00316210.1

Sequence Viewer

Length: 1752 bp
ATGAGAAATTCAAGGAGCTTGAAAGAAAATGAAGAAAGGATTGGGGAAGGGAAACATTTGGTGATGTTGGGAAGGAGAAAGAGGAGGCAAAGGCTATCATTAAGGAGTTGGAATCAGAAGAGATGGACAGAGGGAAGGTTTAGGGAAAATGCAAGTATTATGAGAAATGTAAAGCAATTGGATTATCAGCAGACTGCTGGTATGGGAACAGAAGAGATAAAGAAAGATGCGGATGCCAGCAATTGGCAATCTCATTTGGCATATGATCAGTGGGTAGCACTTCCTGTATCTGGTTCACGACCACCAGCTCGCTACAAGCATGCTGCTGTAGTAGTTGATGAAAAGTTGTATATTGTTGGCGGGAGTCGTAATGGACGGTATCTATCTGATGTTCAGGTCTTTGACTTTAGAAATTTGTTATGGTCAACTATAAAACTGAATGCAAATTCCAATAATTTTGAAGATAGTGGTTTGCAGGAAGTTGTTCCACCCACTTCTTCTCACAATCTGGTGAAGTGGGGAAACAAACTTCTGCTGCTTGGTGGGAATTCAAAGAAGTTGTCTGATAAAATAATAGTGTGGTTCATTGATTTGGAAACACACCTCTGTGGCGTCATGGAGACTTCAGGAAAAGTTCCGCCAGCTAGTGCAGGGCAGTCAACTACACTGGTTGGCTCTAGATTGATAATGTTCGGTGGAGAAGACAGGAGCAGGAAATTGTTGAATGATGTCAATGTTCTTGATTTAGAGACTATGACTTGGGATGTGATGGAGACATTGCAGACTCCTCCAGCTCCCAGATTTGATCACACAGCAGCAGTACATGCAGAACGTTACCTTCTAATTTTTGGTGGCTGTTCACATGCTAGTTTCTTCAATGATCTTCATGTTCTGGACTTGCAAACTATGGAGTGGTCCCAGCCACAAATTCAGGGTGATCTTGTAACTCCTAGGGCAGGTCACGCGGGTATAACTATTGATGAAAACTGGTATATAGTTGGCGGTGGAGATAACAAAAATGGTTGCCCAGAAACCCTGGCCTTAAATATGTCTAAGCTAGTGTGGTCAGTGTCAACAATTGTAAAGCAAAGGGATCCACTCGCTAGTGAGGGTCTCACTGTCTGCTCCGTGAATATTGATGGAAAGAAGCATTTGGTTGCCTTTGGTGGCTATAATGGGAACTATAGCAACGAGGTTTTTGTTATGAGACCCAAACCAAATGACTCTTCACAACGAAAGATATTCCAGTCACCAGCGGCTGCTGCAGCAGCAGCTTCTGTTACTGCTGCCTATGCCTTGACTAAATCAGAAAAATTGAATTTCACAACAGTACAGTCGAACTCCAAGGGAGCTGAAAATCATCGTTCTGAACAAGATTTCACAATTGACATAGAGGCCCTGAGAGAAGAGAAAGGTGTGCTGGAATTGTCTCTTTCCGAAGTCAGGAAAGAAAATGCTAGGGTCAGCCAGGAGATAGATGAAGTCAACAATACTCACAGTGAATTGTCCAAGGAACTGCAGTCAGTCCAAGGGCAACTTGTAGCTGAGAGATCAAGATGCTTTAAATTGGAGGCTCAAATCAGTGAACTACAGAAGATACTAGAATCAGTGCAGTCCATTGAGGATGAGGTACAGGCACTTCGCGGACAGAAATCTGCAATGGAACGGGATATGGAGCTTGCTTCATCTGTTCAGAGACAAGGTTCTGGTGGTGTCTGGAGATGGATTGCTGGTAGTGGTGGCAACACATAA

Protein Analysis

583

Amino Acids

64.96

Weight (kDa)

6.92

Isoelectric Point (pI)

45.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_ATRN-LZTR1 PF24981 73 - 221 1.9e-12 Attractin/LZTR1 beta-propeller
Kelch_KLHDC2_KLHL20_DRC7 PF24681 85 - 147 7.6e-13 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 90 - 148 5.7e-09 FBX42, beta-propeller domain
Kelch_1 PF01344 103 - 144 1.8e-10 Kelch motif
Kelch_2 PF07646 104 - 142 4.7e-06 Kelch motif
Beta-prop_ATRN-LZTR1 PF24981 146 - 289 9.7e-14 Attractin/LZTR1 beta-propeller
Beta-prop_FBX42 PF13415 147 - 288 8.7e-25 FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 167 - 390 9.6e-53 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_HCF PF13854 202 - 335 4.4e-16 Host cell factor, Kelch-repeats domain
Beta-prop_FBX42 PF13415 267 - 423 4.5e-21 FBX42, beta-propeller domain
Beta-prop_ATRN-LZTR1 PF24981 281 - 414 9.8e-12 Attractin/LZTR1 beta-propeller
ACBP4_C PF24922 462 - 562 2.8e-37 Acyl-CoA-binding domain-containing protein 4-6, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 947
AccB7I CCANNNNNTGG 1 cut(s) 243
AccII CGCG 2 cut(s) 965, 1644
AciI CCGC 7 cut(s) 230, 360, 638, 965, 1002, 1256, 1644
AclI AACGTT 1 cut(s) 832
AclWI GGATC 2 cut(s) 1088, 1101
AcsI RAATTY 6 cut(s) 7, 412, 445, 547, 927, 1318
AcuI CTGAAG 1 cut(s) 609
AcyI GRCGYC 1 cut(s) 612
AfaI GTAC 3 cut(s) 822, 1332, 1632
AfiI CCNNNNNNNGG 4 cut(s) 243, 290, 956, 1443
AgsI TTSAA 7 cut(s) 12, 22, 461, 552, 724, 877, 1318
AjnI CCWGG 2 cut(s) 1035, 1467
AjuI GAANNNNNNNTTGG 4 cut(s) 24, 56, 1210, 1242
AleI CACNNNNGTG 1 cut(s) 606
AluBI AGCT 9 cut(s) 18, 308, 644, 794, 1057, 1274, 1352, 1544, 1678
AluI AGCT 9 cut(s) 18, 308, 644, 794, 1057, 1274, 1352, 1544, 1678
Alw26I GTCTC 7 cut(s) 614, 743, 767, 1118, 1201, 1434, 1690
AlwI GGATC 2 cut(s) 1088, 1101
AlwNI CAGNNNCTG 2 cut(s) 1259, 1277
AoxI GGCC 2 cut(s) 1038, 1395
ApeKI GCWGC 9 cut(s) 323, 535, 815, 1259, 1262, 1265, 1268, 1271, 1286
ApoI RAATTY 6 cut(s) 7, 412, 445, 547, 927, 1318
ArsI GACNNNNNNTTYG 2 cut(s) 545, 577
Asp700I GAANNNNTTC 1 cut(s) 483
AspA2I CCTAGG 1 cut(s) 950
AspS9I GGNCC 2 cut(s) 915, 1396
AsuHPI GGTGA 4 cut(s) 73, 523, 947, 1242
AvaII GGWCC 1 cut(s) 915
AvrII CCTAGG 1 cut(s) 950
BamHI GGATCC 1 cut(s) 1093
BarI GAAGNNNNNNTAC 2 cut(s) 1623, 1655
BbsI GAAGAC 1 cut(s) 708
BbvI GCAGC 9 cut(s) 310, 522, 827, 1246, 1249, 1273, 1277, 1280, 1283
BccI CCATC 4 cut(s) 117, 763, 1133, 1716
BciT130I CCWGG 2 cut(s) 1037, 1469
BclI TGATCA 2 cut(s) 265, 805
BcoDI GTCTC 7 cut(s) 614, 743, 767, 1118, 1201, 1434, 1690
BfaI CTAG 8 cut(s) 645, 678, 867, 951, 1058, 1104, 1458, 1601
BfmI CTRYAG 5 cut(s) 327, 1183, 1263, 1517, 1589
BfuAI ACCTGC 1 cut(s) 947
BlnI CCTAGG 1 cut(s) 950
Bme1390I CCNGG 2 cut(s) 1037, 1469
Bme18I GGWCC 1 cut(s) 915
BmgT120I GGNCC 2 cut(s) 915, 1396
BmiI GGNNCC 2 cut(s) 917, 1095
BmrFI CCNGG 2 cut(s) 1037, 1469
BmsI GCATC 3 cut(s) 217, 223, 1547
BpiI GAAGAC 1 cut(s) 708
BpmI CTGGAG 2 cut(s) 774, 1738
BsaHI GRCGYC 1 cut(s) 612
BsaI GGTCTC 2 cut(s) 1118, 1201
BsaJI CCNNGG 5 cut(s) 950, 1035, 1344, 1509, 1528
Bsc4I CCNNNNNNNGG 4 cut(s) 243, 290, 956, 1443
Bse1I ACTGG 3 cut(s) 672, 992, 1246
Bse3DI GCAATG 2 cut(s) 776, 1665
BseBI CCWGG 2 cut(s) 1037, 1469
BseDI CCNNGG 5 cut(s) 950, 1035, 1344, 1509, 1528
BseGI GGATG 3 cut(s) 238, 769, 1630
BseLI CCNNNNNNNGG 4 cut(s) 243, 290, 956, 1443
BseMI GCAATG 2 cut(s) 776, 1665
BseMII CTCAG 2 cut(s) 1391, 1536
BseNI ACTGG 3 cut(s) 672, 992, 1246
BseRI GAGGAG 2 cut(s) 97, 777
BseXI GCAGC 9 cut(s) 310, 522, 827, 1246, 1249, 1273, 1277, 1280, 1283
BseYI CCCAGC 1 cut(s) 918
BsgI GTGCAG 2 cut(s) 669, 1631
Bsh1236I CGCG 2 cut(s) 965, 1644
BshFI GGCC 2 cut(s) 1040, 1397
BslFI GGGAC 1 cut(s) 901
BslI CCNNNNNNNGG 4 cut(s) 243, 290, 956, 1443
BsmAI GTCTC 7 cut(s) 614, 743, 767, 1118, 1201, 1434, 1690
BsmFI GGGAC 1 cut(s) 901
BsmI GAATGC 1 cut(s) 445
BsnI GGCC 2 cut(s) 1040, 1397
Bso31I GGTCTC 2 cut(s) 1118, 1201
Bsp143I GATC 6 cut(s) 265, 805, 880, 937, 1093, 1550
BspACI CCGC 7 cut(s) 230, 360, 638, 965, 1002, 1256, 1644
BspANI GGCC 2 cut(s) 1040, 1397
BspCNI CTCAG 2 cut(s) 1392, 1537
BspFNI CGCG 2 cut(s) 965, 1644
BspLI GGNNCC 2 cut(s) 917, 1095
BspMAI CTGCAG 2 cut(s) 1267, 1521
BspMI ACCTGC 1 cut(s) 947
BspPI GGATC 2 cut(s) 1088, 1101
BspTNI GGTCTC 2 cut(s) 1118, 1201
BsrDI GCAATG 2 cut(s) 776, 1665
BsrI ACTGG 3 cut(s) 672, 992, 1246
BssECI CCNNGG 5 cut(s) 950, 1035, 1344, 1509, 1528
BssMI GATC 6 cut(s) 265, 805, 880, 937, 1093, 1550
BssNI GRCGYC 1 cut(s) 612
BssT1I CCWWGG 4 cut(s) 950, 1344, 1509, 1528
Bst2UI CCWGG 2 cut(s) 1037, 1469
Bst4CI ACNGT 5 cut(s) 378, 1120, 1330, 1335, 1499
Bst6I CTCTTC 4 cut(s) 113, 207, 1231, 1401
BstACI GRCGYC 1 cut(s) 612
BstAPI GCANNNNNTGC 1 cut(s) 824
BstC8I GCNNGC 5 cut(s) 238, 310, 321, 642, 1680
BstDEI CTNAG 3 cut(s) 1053, 1400, 1545
BstENI CCTNNNNNAGG 1 cut(s) 954
BstF5I GGATG 3 cut(s) 238, 769, 1630
BstFNI CGCG 2 cut(s) 965, 1644
BstKTI GATC 6 cut(s) 268, 808, 883, 940, 1096, 1553
BstMAI GTCTC 7 cut(s) 614, 743, 767, 1118, 1201, 1434, 1690
BstMBI GATC 6 cut(s) 265, 805, 880, 937, 1093, 1550
BstMWI GCNNNNNNNGC 7 cut(s) 824, 962, 1262, 1265, 1268, 1271, 1292
BstNI CCWGG 2 cut(s) 1037, 1469
BstNSI RCATGY 3 cut(s) 323, 827, 866
BstSCI CCNGG 2 cut(s) 1035, 1467
BstSFI CTRYAG 5 cut(s) 327, 1183, 1263, 1517, 1589
BstUI CGCG 2 cut(s) 965, 1644
BstV1I GCAGC 9 cut(s) 310, 522, 827, 1246, 1249, 1273, 1277, 1280, 1283
BstV2I GAAGAC 1 cut(s) 708
BstX2I RGATCY 1 cut(s) 1093
BstYI RGATCY 1 cut(s) 1093
BsuRI GGCC 2 cut(s) 1040, 1397
BtsCI GGATG 3 cut(s) 238, 769, 1630
BtsIMutI CAGTG 7 cut(s) 275, 665, 1074, 1116, 1504, 1588, 1614
BveI ACCTGC 1 cut(s) 947
Cac8I GCNNGC 5 cut(s) 238, 310, 321, 642, 1680
CaiI CAGNNNCTG 2 cut(s) 1259, 1277
Cfr13I GGNCC 2 cut(s) 915, 1396
CseI GACGC 1 cut(s) 601
Csp6I GTAC 3 cut(s) 821, 1331, 1631
CviAII CATG 5 cut(s) 320, 616, 824, 863, 887
CviQI GTAC 3 cut(s) 821, 1331, 1631
DdeI CTNAG 3 cut(s) 1053, 1400, 1545
DpnI GATC 6 cut(s) 267, 807, 882, 939, 1095, 1552
DpnII GATC 6 cut(s) 265, 805, 880, 937, 1093, 1550
DraI TTTAAA 1 cut(s) 1564
Eam1104I CTCTTC 4 cut(s) 113, 207, 1231, 1401
EarI CTCTTC 4 cut(s) 113, 207, 1231, 1401
EciI GGCGGA 1 cut(s) 627
Eco130I CCWWGG 4 cut(s) 950, 1344, 1509, 1528
Eco31I GGTCTC 2 cut(s) 1118, 1201
Eco47I GGWCC 1 cut(s) 915
Eco57I CTGAAG 1 cut(s) 609
EcoNI CCTNNNNNAGG 1 cut(s) 954
EcoO109I RGGNCCY 1 cut(s) 1396
EcoRI GAATTC 1 cut(s) 547
EcoRII CCWGG 2 cut(s) 1035, 1467
EcoT14I CCWWGG 4 cut(s) 950, 1344, 1509, 1528
ErhI CCWWGG 4 cut(s) 950, 1344, 1509, 1528
FaeI CATG 5 cut(s) 323, 619, 827, 866, 890
FalI AAGNNNNNCTT 2 cut(s) 1521, 1553
FaqI GGGAC 1 cut(s) 901
FatI CATG 5 cut(s) 319, 615, 823, 862, 886
FauI CCCGC 2 cut(s) 353, 958
FauNDI CATATG 1 cut(s) 262
FbaI TGATCA 2 cut(s) 265, 805
FokI GGATG 3 cut(s) 245, 776, 1637
FspBI CTAG 8 cut(s) 645, 678, 867, 951, 1058, 1104, 1458, 1601
GsaI CCCAGC 1 cut(s) 922
GsuI CTGGAG 2 cut(s) 774, 1738
HaeIII GGCC 2 cut(s) 1040, 1397
HgaI GACGC 1 cut(s) 601
Hin1I GRCGYC 1 cut(s) 612
Hin1II CATG 5 cut(s) 323, 619, 827, 866, 890
HincII GTYRAC 4 cut(s) 426, 660, 1074, 1486
HindII GTYRAC 4 cut(s) 426, 660, 1074, 1486
HinfI GANTC 5 cut(s) 112, 364, 784, 1223, 1604
HphI GGTGA 4 cut(s) 73, 523, 947, 1242
Hpy166II GTNNAC 7 cut(s) 296, 426, 660, 860, 1074, 1486, 1586
Hpy188I TCNGA 7 cut(s) 117, 388, 565, 1309, 1369, 1438, 1695
Hpy188III TCNNGA 8 cut(s) 297, 627, 678, 740, 893, 1444, 1554, 1717
Hpy8I GTNNAC 7 cut(s) 296, 426, 660, 860, 1074, 1486, 1586
HpyAV CCTTC 4 cut(s) 41, 66, 129, 848
HpyCH4III ACNGT 5 cut(s) 378, 1120, 1330, 1335, 1499
HpyCH4IV ACGT 1 cut(s) 832
HpyF10VI GCNNNNNNNGC 7 cut(s) 824, 962, 1262, 1265, 1268, 1271, 1292
HpyF3I CTNAG 3 cut(s) 1053, 1400, 1545
HpySE526I ACGT 1 cut(s) 832
Hsp92I GRCGYC 1 cut(s) 612
Hsp92II CATG 5 cut(s) 323, 619, 827, 866, 890
Ksp22I TGATCA 2 cut(s) 265, 805
Kzo9I GATC 6 cut(s) 265, 805, 880, 937, 1093, 1550
LmnI GCTCC 6 cut(s) 15, 708, 799, 1130, 1349, 1675
Lsp1109I GCAGC 9 cut(s) 310, 522, 827, 1246, 1249, 1273, 1277, 1280, 1283
LweI GCATC 3 cut(s) 217, 223, 1547
MaeI CTAG 8 cut(s) 645, 678, 867, 951, 1058, 1104, 1458, 1601
MaeII ACGT 1 cut(s) 832
MaeIII GTNAC 5 cut(s) 833, 943, 959, 1248, 1279
MalI GATC 6 cut(s) 267, 807, 882, 939, 1095, 1552
MboI GATC 6 cut(s) 265, 805, 880, 937, 1093, 1550
MfeI CAATTG 4 cut(s) 176, 241, 1077, 1383
MflI RGATCY 1 cut(s) 1093
MlyI GAGTC 3 cut(s) 373, 778, 1217
MmeI TCCRAC 1 cut(s) 89
MroXI GAANNNNTTC 1 cut(s) 483
MseI TTAA 3 cut(s) 101, 1043, 1563
MslI CAYNNNNRTG 1 cut(s) 606
MspA1I CMGCKG 1 cut(s) 1256
MspR9I CCNGG 2 cut(s) 1037, 1469
MunI CAATTG 4 cut(s) 176, 241, 1077, 1383
Mva1269I GAATGC 1 cut(s) 445
MvaI CCWGG 2 cut(s) 1037, 1469
MvnI CGCG 2 cut(s) 965, 1644
MwoI GCNNNNNNNGC 7 cut(s) 824, 962, 1262, 1265, 1268, 1271, 1292
NdeI CATATG 1 cut(s) 262
NdeII GATC 6 cut(s) 265, 805, 880, 937, 1093, 1550
NlaIII CATG 5 cut(s) 323, 619, 827, 866, 890
NlaIV GGNNCC 2 cut(s) 917, 1095
NmuCI GTSAC 2 cut(s) 959, 1248
NspI RCATGY 3 cut(s) 323, 827, 866
OliI CACNNNNGTG 1 cut(s) 606
PaeI GCATGC 1 cut(s) 323
PctI GAATGC 1 cut(s) 445
PdmI GAANNNNTTC 1 cut(s) 483
PfeI GAWTC 2 cut(s) 112, 1604
PflMI CCANNNNNTGG 1 cut(s) 243
PleI GAGTC 3 cut(s) 372, 778, 1217
PpsI GAGTC 3 cut(s) 372, 778, 1217
Psp1406I AACGTT 1 cut(s) 832
Psp6I CCWGG 2 cut(s) 1035, 1467
PspFI CCCAGC 1 cut(s) 918
PspGI CCWGG 2 cut(s) 1035, 1467
PspN4I GGNNCC 2 cut(s) 917, 1095
PspPI GGNCC 2 cut(s) 915, 1396
PstI CTGCAG 2 cut(s) 1267, 1521
PstNI CAGNNNCTG 2 cut(s) 1259, 1277
PsuI RGATCY 1 cut(s) 1093
RsaI GTAC 3 cut(s) 822, 1332, 1632
RsaNI GTAC 3 cut(s) 821, 1331, 1631
RseI CAYNNNNRTG 1 cut(s) 606
SaqAI TTAA 3 cut(s) 101, 1043, 1563
Sau3AI GATC 6 cut(s) 265, 805, 880, 937, 1093, 1550
Sau96I GGNCC 2 cut(s) 915, 1396
SchI GAGTC 3 cut(s) 373, 778, 1217
ScrFI CCNGG 2 cut(s) 1037, 1469
SfaNI GCATC 3 cut(s) 217, 223, 1547
SfcI CTRYAG 5 cut(s) 327, 1183, 1263, 1517, 1589
SinI GGWCC 1 cut(s) 915
SmiMI CAYNNNNRTG 1 cut(s) 606
SphI GCATGC 1 cut(s) 323
SsiI CCGC 7 cut(s) 230, 360, 638, 965, 1002, 1256, 1644
SspI AATATT 1 cut(s) 1135
SspMI CTAG 8 cut(s) 645, 678, 867, 951, 1058, 1104, 1458, 1601
StyD4I CCNGG 2 cut(s) 1035, 1467
StyI CCWWGG 4 cut(s) 950, 1344, 1509, 1528
TaaI ACNGT 5 cut(s) 378, 1120, 1330, 1335, 1499
TaiI ACGT 1 cut(s) 835
TaqI TCGA 1 cut(s) 1337
TatI WGTACW 2 cut(s) 820, 1330
TauI GCSGC 1 cut(s) 1259
TfiI GAWTC 2 cut(s) 112, 1604
Tru1I TTAA 3 cut(s) 101, 1043, 1563
Tru9I TTAA 3 cut(s) 101, 1043, 1563
TscAI CASTG 7 cut(s) 275, 672, 1074, 1123, 1504, 1588, 1614
TseFI GTSAC 2 cut(s) 959, 1248
TseI GCWGC 9 cut(s) 323, 535, 815, 1259, 1262, 1265, 1268, 1271, 1286
Tsp45I GTSAC 2 cut(s) 959, 1248
TspDTI ATGAA 7 cut(s) 45, 354, 574, 875, 996, 1494, 1674
TspGWI ACGGA 1 cut(s) 1117
TspRI CASTG 7 cut(s) 275, 672, 1074, 1123, 1504, 1588, 1614
Van91I CCANNNNNTGG 1 cut(s) 243
VpaK11BI GGWCC 1 cut(s) 915
XagI CCTNNNNNAGG 1 cut(s) 954
XapI RAATTY 6 cut(s) 7, 412, 445, 547, 927, 1318
XbaI TCTAGA 1 cut(s) 677
XceI RCATGY 3 cut(s) 323, 827, 866
XmaJI CCTAGG 1 cut(s) 950
XmnI GAANNNNTTC 1 cut(s) 483
XspI CTAG 8 cut(s) 645, 678, 867, 951, 1058, 1104, 1458, 1601
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.