AT5G04890

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0013354
Physical Location & Seq
Forward (+)
1427117 .. 1428514
1398 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G04890.1

Sequence Viewer

Length: 1101 bp
ATGGCAGCGAGACAGCAACAAAAAGGAACAGGCTTCGGGGTTCAATACGAGGACTTTGTCCCCAAATCCGAATGGAAAGATCAACCTGAAGCCACCATTCTCAACATTGACCTAACAGGTTTTGCAAAGGAGCAAATGAAAGTGACGTATGTGCACAGCTCGAAGATGATAAGAGTCACTGGAGAACGTCCCTTGGCTAATCGGAAATGGAGCCGTTTCAACGAGGTGTTCACTGTTCCACAGAATTGTTTAGTGGATAAGATCCATGGGAGCTTTAAGAACAATGTCCTCACCATCACCATGCCTAAGGAGACGATCACGAAGGTGGCTTATCTTCCAGAAACTTCTAGAACTGAAGCTGCTGCTCTGGAGAAGGCGGCGAAGCTAGAGGAGAAGAGATTGTTAGAAGAATCTAGAAGGAAAGAGAAGGAAGAAGAAGAAGCTAAGCAGATGAAGAAGCAGCTTCTGGAAGAGAAAGAGGCGCTAATTAGGAAGCTGCAAGAAGAAGCTAAAGCAAAGGAAGAGGCTGAAATGAGGAAGCTTCAAGAAGAAGCTAAAGCAAAGGAGGAGGCCGCGGCAAAGAAGCTTCAAGAAGAAATTGAAGCAAAAGAGAAACTTGAGGAGAGAAAGCTCGAGGAAAGGAGACTTGAGGAGAGGAAGCTCGAGGATATGAAGCTTGCGGAAGAAGCTAAACTAAAGAAGATCCAGGAGAGAAAGTCTGTTGATGAATCTGGAGAAAAAGAGAAGATACTAAAGCCAGAAGTGGTCTATACAAAATCCGGGCACGTGGCTACTCCCAAACCTGAGTCGGGGTCTGGTCTCAAGTCCGGGTTTGGAGGAGTAGGCGAGGTGGTGAAGTCAGCGGAAGAAAAACTAGGAAACTTGGTGGAAAAGGAAAAGAAAATGGGGAAAGGGATAATGGAGAAAATAAGAAGGAAAGAGATAACAAGTGAAGAGAAGAAGTTGATGATGAATGTAGGAGTGGCTGCTCTTGTTATCTTTGCTCTCGGAGCTTATGTTTCTTATACCTTCTGCTCTTCTTCTTCTTCTTCTTCCTCTTCGTCTCCTTCTTCATCATCATCTTCTACCAAACCAGAATGA

Protein Analysis

366

Amino Acids

41.34

Weight (kDa)

8.87

Isoelectric Point (pI)

56.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 25 - 105 9e-09 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013354)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G04890
fragaria_vesca FvH4_7g05240
malus_domestica MD02G1259500.v1.1 MD07G1061000.v1.1 MD07G1061100.v1.1
prunus_persica Prupe.2G065600_v2.0.a1 Prupe.2G065600_v2.0.a1
pyrus_communis pycom02g22090
rosa_chinensis RchiOBHm_Chr1g0331581
rosa_laevigata RLG00000029728
rosa_multiflora Rmu_sc0008675.1_g000002
rosa_roxburghii Rroxscaffold_4G00319040
rosa_rugosa Rorug01G0094900
rosa_samantha Rh1AG118700 Rh1BG090400 Rh1CG113700 Rh1DG122900
rosa_wichuraiana Rw1G009510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 575
AciI CCGC 5 cut(s) 377, 573, 575, 680, 863
AclWI GGATC 2 cut(s) 256, 697
AcuI CTGAAG 2 cut(s) 108, 375
AcvI CACGTG 1 cut(s) 787
AfiI CCNNNNNNNGG 1 cut(s) 809
AgsI TTSAA 5 cut(s) 44, 220, 545, 590, 602
AjnI CCWGG 1 cut(s) 705
AleI CACNNNNGTG 1 cut(s) 323
Alw21I GWGCWC 1 cut(s) 156
Alw26I GTCTC 5 cut(s) 4, 305, 637, 824, 1068
Alw44I GTGCAC 1 cut(s) 152
AlwI GGATC 2 cut(s) 256, 697
AlwNI CAGNNNCTG 1 cut(s) 466
Ama87I CYCGRG 2 cut(s) 632, 662
AoxI GGCC 1 cut(s) 570
ApaLI GTGCAC 1 cut(s) 152
ApeKI GCWGC 6 cut(s) 5, 359, 362, 460, 496, 986
AspLEI GCGC 1 cut(s) 484
AsuC2I CCSGG 2 cut(s) 781, 829
AsuHPI GGTGA 3 cut(s) 283, 289, 865
AvaI CYCGRG 2 cut(s) 632, 662
AxyI CCTNAGG 1 cut(s) 306
BaeGI GKGCMC 2 cut(s) 156, 786
BbrPI CACGTG 1 cut(s) 787
Bbv12I GWGCWC 1 cut(s) 156
BbvI GCAGC 6 cut(s) 17, 346, 349, 472, 483, 973
BccI CCATC 1 cut(s) 302
BceAI ACGGC 1 cut(s) 198
BciT130I CCWGG 1 cut(s) 707
BcnI CCSGG 2 cut(s) 781, 829
BcoDI GTCTC 5 cut(s) 4, 305, 637, 824, 1068
BfaI CTAG 4 cut(s) 348, 386, 414, 875
BfoI RGCGCY 1 cut(s) 485
BisI GCNGC 9 cut(s) 6, 360, 363, 378, 461, 497, 573, 576, 987
BlpI GCTNAGC 1 cut(s) 444
BlsI GCNGC 9 cut(s) 7, 361, 364, 379, 462, 498, 574, 577, 988
Bme1390I CCNGG 3 cut(s) 707, 781, 829
BmeT110I CYCGRG 2 cut(s) 632, 662
BmiI GGNNCC 1 cut(s) 212
BmrFI CCNGG 3 cut(s) 707, 781, 829
BpmI CTGGAG 3 cut(s) 201, 389, 753
Bpu1102I GCTNAGC 1 cut(s) 444
BpuEI CTTGAG 3 cut(s) 638, 668, 806
BpuMI CCSGG 2 cut(s) 781, 829
BsaAI YACGTR 1 cut(s) 787
BsaI GGTCTC 1 cut(s) 824
BsaJI CCNNGG 3 cut(s) 192, 265, 573
BsaXI ACNNNNNCTCC 4 cut(s) 701, 731, 1002, 1032
Bsc4I CCNNNNNNNGG 1 cut(s) 809
Bse1I ACTGG 1 cut(s) 184
Bse21I CCTNAGG 1 cut(s) 306
BseBI CCWGG 1 cut(s) 707
BseDI CCNNGG 3 cut(s) 192, 265, 573
BseLI CCNNNNNNNGG 1 cut(s) 809
BseMII CTCAG 1 cut(s) 795
BseNI ACTGG 1 cut(s) 184
BseRI GAGGAG 5 cut(s) 404, 581, 635, 665, 852
BseSI GKGCMC 2 cut(s) 156, 786
BseXI GCAGC 6 cut(s) 17, 346, 349, 472, 483, 973
Bsh1236I CGCG 1 cut(s) 575
BshFI GGCC 1 cut(s) 572
BsiHKAI GWGCWC 1 cut(s) 156
BsiHKCI CYCGRG 2 cut(s) 632, 662
BsiSI CCGG 2 cut(s) 780, 828
BslFI GGGAC 2 cut(s) 44, 174
BslI CCNNNNNNNGG 1 cut(s) 809
BsmAI GTCTC 5 cut(s) 4, 305, 637, 824, 1068
BsmBI CGTCTC 2 cut(s) 305, 1068
BsmFI GGGAC 2 cut(s) 44, 174
BsnI GGCC 1 cut(s) 572
Bso31I GGTCTC 1 cut(s) 824
BsoBI CYCGRG 2 cut(s) 632, 662
Bsp1286I GDGCHC 2 cut(s) 156, 786
Bsp143I GATC 4 cut(s) 79, 261, 315, 702
Bsp1720I GCTNAGC 1 cut(s) 444
Bsp19I CCATGG 1 cut(s) 265
BspACI CCGC 5 cut(s) 377, 573, 575, 680, 863
BspANI GGCC 1 cut(s) 572
BspCNI CTCAG 1 cut(s) 796
BspFNI CGCG 1 cut(s) 575
BspLI GGNNCC 1 cut(s) 212
BspPI GGATC 2 cut(s) 256, 697
BspQI GCTCTTC 1 cut(s) 1042
BspTNI GGTCTC 1 cut(s) 824
BsrI ACTGG 1 cut(s) 184
BssECI CCNNGG 3 cut(s) 192, 265, 573
BssMI GATC 4 cut(s) 79, 261, 315, 702
BssT1I CCWWGG 2 cut(s) 192, 265
Bst2UI CCWGG 1 cut(s) 707
Bst4CI ACNGT 1 cut(s) 235
Bst6I CTCTTC 6 cut(s) 389, 465, 516, 948, 1042, 1063
BstBAI YACGTR 1 cut(s) 787
BstC8I GCNNGC 1 cut(s) 678
BstDEI CTNAG 3 cut(s) 306, 444, 804
BstDSI CCRYGG 2 cut(s) 265, 573
BstFNI CGCG 1 cut(s) 575
BstH2I RGCGCY 1 cut(s) 485
BstHHI GCGC 1 cut(s) 484
BstKTI GATC 4 cut(s) 82, 264, 318, 705
BstMAI GTCTC 5 cut(s) 4, 305, 637, 824, 1068
BstMBI GATC 4 cut(s) 79, 261, 315, 702
BstMWI GCNNNNNNNGC 2 cut(s) 686, 1010
BstNI CCWGG 1 cut(s) 707
BstSCI CCNGG 3 cut(s) 705, 779, 827
BstSLI GKGCMC 2 cut(s) 156, 786
BstUI CGCG 1 cut(s) 575
BstV1I GCAGC 6 cut(s) 17, 346, 349, 472, 483, 973
BstX2I RGATCY 2 cut(s) 261, 702
BstYI RGATCY 2 cut(s) 261, 702
Bsu36I CCTNAGG 1 cut(s) 306
BsuRI GGCC 1 cut(s) 572
BtgI CCRYGG 2 cut(s) 265, 573
BtsIMutI CAGTG 2 cut(s) 177, 231
Cac8I GCNNGC 1 cut(s) 678
CaiI CAGNNNCTG 1 cut(s) 466
CfoI GCGC 1 cut(s) 484
Cfr42I CCGCGG 1 cut(s) 576
CviAII CATG 2 cut(s) 266, 301
DdeI CTNAG 3 cut(s) 306, 444, 804
DpnI GATC 4 cut(s) 81, 263, 317, 704
DpnII GATC 4 cut(s) 79, 261, 315, 702
Eam1104I CTCTTC 6 cut(s) 389, 465, 516, 948, 1042, 1063
EarI CTCTTC 6 cut(s) 389, 465, 516, 948, 1042, 1063
Eco130I CCWWGG 2 cut(s) 192, 265
Eco31I GGTCTC 1 cut(s) 824
Eco57I CTGAAG 2 cut(s) 108, 375
Eco72I CACGTG 1 cut(s) 787
Eco81I CCTNAGG 1 cut(s) 306
Eco88I CYCGRG 2 cut(s) 632, 662
EcoRII CCWGG 1 cut(s) 705
EcoT14I CCWWGG 2 cut(s) 192, 265
ErhI CCWWGG 2 cut(s) 192, 265
Esp3I CGTCTC 2 cut(s) 305, 1068
FaeI CATG 2 cut(s) 269, 304
FaiI YATR 7 cut(s) 150, 267, 302, 671, 771, 1017, 1026
FalI AAGNNNNNCTT 2 cut(s) 600, 632
FaqI GGGAC 2 cut(s) 44, 174
FatI CATG 2 cut(s) 265, 300
Fnu4HI GCNGC 9 cut(s) 6, 360, 363, 378, 461, 497, 573, 576, 987
Fsp4HI GCNGC 9 cut(s) 6, 360, 363, 378, 461, 497, 573, 576, 987
FspBI CTAG 4 cut(s) 348, 386, 414, 875
GlaI GCGC 1 cut(s) 483
GluI GCNGC 9 cut(s) 6, 360, 363, 378, 461, 497, 573, 576, 987
GsuI CTGGAG 3 cut(s) 201, 389, 753
HaeII RGCGCY 1 cut(s) 485
HaeIII GGCC 1 cut(s) 572
HapII CCGG 2 cut(s) 780, 828
HhaI GCGC 1 cut(s) 484
Hin1II CATG 2 cut(s) 269, 304
Hin6I GCGC 1 cut(s) 482
HinP1I GCGC 1 cut(s) 482
HindIII AAGCTT 3 cut(s) 539, 584, 674
HinfI GANTC 4 cut(s) 174, 410, 728, 806
HpaII CCGG 2 cut(s) 780, 828
HphI GGTGA 3 cut(s) 283, 289, 865
Hpy166II GTNNAC 2 cut(s) 154, 231
Hpy188I TCNGA 3 cut(s) 70, 204, 1010
Hpy188III TCNNGA 9 cut(s) 319, 338, 348, 368, 414, 467, 545, 590, 732
Hpy8I GTNNAC 2 cut(s) 154, 231
HpyAV CCTTC 7 cut(s) 316, 367, 411, 421, 927, 1039, 1077
HpyCH4III ACNGT 1 cut(s) 235
HpyCH4IV ACGT 3 cut(s) 146, 187, 786
HpyCH4V TGCA 3 cut(s) 125, 154, 499
HpyF10VI GCNNNNNNNGC 2 cut(s) 686, 1010
HpyF3I CTNAG 3 cut(s) 306, 444, 804
HpySE526I ACGT 3 cut(s) 146, 187, 786
Hsp92II CATG 2 cut(s) 269, 304
HspAI GCGC 1 cut(s) 482
KspI CCGCGG 1 cut(s) 576
Kzo9I GATC 4 cut(s) 79, 261, 315, 702
LguI GCTCTTC 1 cut(s) 1042
LmnI GCTCC 4 cut(s) 130, 210, 270, 1010
Lsp1109I GCAGC 6 cut(s) 17, 346, 349, 472, 483, 973
MaeI CTAG 4 cut(s) 348, 386, 414, 875
MaeII ACGT 3 cut(s) 146, 187, 786
MaeIII GTNAC 2 cut(s) 142, 175
MalI GATC 4 cut(s) 81, 263, 317, 704
MboI GATC 4 cut(s) 79, 261, 315, 702
MflI RGATCY 2 cut(s) 261, 702
MhlI GDGCHC 2 cut(s) 156, 786
MluCI AATT 3 cut(s) 244, 486, 597
MlyI GAGTC 2 cut(s) 183, 815
MseI TTAA 1 cut(s) 276
MslI CAYNNNNRTG 2 cut(s) 299, 323
MspA1I CMGCKG 2 cut(s) 575, 863
MspI CCGG 2 cut(s) 780, 828
MspR9I CCNGG 3 cut(s) 707, 781, 829
MvaI CCWGG 1 cut(s) 707
MvnI CGCG 1 cut(s) 575
MwoI GCNNNNNNNGC 2 cut(s) 686, 1010
NciI CCSGG 2 cut(s) 781, 829
NcoI CCATGG 1 cut(s) 265
NdeII GATC 4 cut(s) 79, 261, 315, 702
NlaIII CATG 2 cut(s) 269, 304
NlaIV GGNNCC 1 cut(s) 212
NmuCI GTSAC 2 cut(s) 142, 175
OliI CACNNNNGTG 1 cut(s) 323
PaeR7I CTCGAG 2 cut(s) 632, 662
PciSI GCTCTTC 1 cut(s) 1042
PfeI GAWTC 2 cut(s) 410, 728
PflFI GACNNNGTC 1 cut(s) 56
PfoI TCCNGGA 1 cut(s) 705
PkrI GCNGC 9 cut(s) 7, 361, 364, 379, 462, 498, 574, 577, 988
PleI GAGTC 2 cut(s) 182, 814
PmaCI CACGTG 1 cut(s) 787
PmlI CACGTG 1 cut(s) 787
PpsI GAGTC 2 cut(s) 182, 814
Ppu21I YACGTR 1 cut(s) 787
Psp6I CCWGG 1 cut(s) 705
PspCI CACGTG 1 cut(s) 787
PspGI CCWGG 1 cut(s) 705
PspN4I GGNNCC 1 cut(s) 212
PspXI VCTCGAGB 2 cut(s) 632, 662
PstNI CAGNNNCTG 1 cut(s) 466
PsuI RGATCY 2 cut(s) 261, 702
PsyI GACNNNGTC 1 cut(s) 56
RseI CAYNNNNRTG 2 cut(s) 299, 323
SacII CCGCGG 1 cut(s) 576
SapI GCTCTTC 1 cut(s) 1042
SaqAI TTAA 1 cut(s) 276
SatI GCNGC 9 cut(s) 6, 360, 363, 378, 461, 497, 573, 576, 987
Sau3AI GATC 4 cut(s) 79, 261, 315, 702
SchI GAGTC 2 cut(s) 183, 815
ScrFI CCNGG 3 cut(s) 707, 781, 829
SduI GDGCHC 2 cut(s) 156, 786
Sfr274I CTCGAG 2 cut(s) 632, 662
Sfr303I CCGCGG 1 cut(s) 576
SgrBI CCGCGG 1 cut(s) 576
SlaI CTCGAG 2 cut(s) 632, 662
SmiMI CAYNNNNRTG 2 cut(s) 299, 323
SmlI CTYRAG 5 cut(s) 617, 632, 647, 662, 821
SmoI CTYRAG 5 cut(s) 617, 632, 647, 662, 821
Sse9I AATT 3 cut(s) 244, 486, 597
SsiI CCGC 5 cut(s) 377, 573, 575, 680, 863
SspMI CTAG 4 cut(s) 348, 386, 414, 875
StyD4I CCNGG 3 cut(s) 705, 779, 827
StyI CCWWGG 2 cut(s) 192, 265
TaaI ACNGT 1 cut(s) 235
TaiI ACGT 3 cut(s) 149, 190, 789
TaqI TCGA 3 cut(s) 161, 633, 663
TasI AATT 3 cut(s) 244, 486, 597
TauI GCSGC 3 cut(s) 380, 575, 578
TfiI GAWTC 2 cut(s) 410, 728
Tru1I TTAA 1 cut(s) 276
Tru9I TTAA 1 cut(s) 276
TscAI CASTG 2 cut(s) 184, 238
TseFI GTSAC 2 cut(s) 142, 175
TseI GCWGC 6 cut(s) 5, 359, 362, 460, 496, 986
Tsp45I GTSAC 2 cut(s) 142, 175
TspDTI ATGAA 6 cut(s) 152, 467, 686, 741, 986, 1062
TspRI CASTG 2 cut(s) 184, 238
Tth111I GACNNNGTC 1 cut(s) 56
VneI GTGCAC 1 cut(s) 152
XbaI TCTAGA 2 cut(s) 347, 413
XhoI CTCGAG 2 cut(s) 632, 662
XspI CTAG 4 cut(s) 348, 386, 414, 875
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.