RLG00000029728

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0013354
Physical Location & Seq
Forward (+)
47878640 .. 47879922
1283 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000029728

Sequence Viewer

Length: 1185 bp
ATGGCTATGAGGCAACGCGTTGGTGCTACTAATACACGTCCAAGACAATCCATTCGTCCCACTTATGAGGATTTCCAGCCCCAGTTTGAATTGAAGGAAGAACCTGAAGCTCACATCATATATGTTCAACTTCCTGGTTTTTTGAAAGAGCAAGTAAGGATTACATTTGTGCACGCCCCTGGTGTGATCAGGGTCCATGGACAACGACCCCTTGGAAACAACATATGGAGCCGTTTCAACCAAACCTTTCCTCTTCCACAAAATTGCCACGAAAGTAAAATTCATGGAAAGTTTAACAATGGCGTTCTTACCATTACATTGCCAAAGGAAGTCCCAAAAGTAGATCCTAAAGAAGCCGCAGCTGCAGCCAAGCCAATCCAAGAGACAAAGCCAAAAACCGCTGCTGCTACTGAGGCAAAGCCACAGAAAGTCTCGGAGGATGACAAGGTTCCTTCGAAAGAGAAGGACCAGATACGTGCTCCTCCATCTCAAAAACCTGCTGCTACTGCTGCCCGGGACGAAAAACAAAAGGATAAGATAAGTATTCCATCTAAAGACCCCCAAGTAGCTGCTCCTCCATCTCAAAAACCAGCTGTCGAGCCCAATTTACAAAAGGGTCAGGATGCCATTCACCAAACAGCTATTGCTGCTGCTGCTAAAGACCCCCAAGTAGCTGCTCCAGAGCCAAAGGCACAAAGGGATGAGAAGAGGGTGATCACATTGAGTAGTGCTGCAAGTGAGCCCAAACCTCAAAAGGGTAGGGATGAAATTCCACCAAAAACTATGGGAGATGAAAAGGCGAAGGCGGACTACAAAAAGGAGGTTCAGGATAGGTACATAAGCGAGGAAAATGAGAAGCCGGGAGAACCAAAGAATGCTGAAAATGTTGTCGACACACCTCTGAGTAAGATGACTGGTGAGAGAAGCACAGAAGAAGCTGTGATCGAAAGGTTTGCCCCAAATACTTTAGATCAGATAAAGAAGAAGAGCAAACGTTTTGGCAGTGGTGTTGGGGAACAGGCTTGCAACTCTGCAAAGCAAGGGATAAAAAATATAGACAAGTGGGTAAATAATGAAGAAAAGCAGCTGTTAGTCAATGTGGGGGCAGCAATTCTTGTTCTTGTGGCATTTGGGGCTTACACGACCTACAACTTATGGTGCTCCTCCGGAAAACCCAAGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

395

Amino Acids

43.5

Weight (kDa)

9.33

Isoelectric Point (pI)

44.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 31 - 118 1.6e-11 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013354)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G04890
fragaria_vesca FvH4_7g05240
malus_domestica MD02G1259500.v1.1 MD07G1061000.v1.1 MD07G1061100.v1.1
prunus_persica Prupe.2G065600_v2.0.a1 Prupe.2G065600_v2.0.a1
pyrus_communis pycom02g22090
rosa_chinensis RchiOBHm_Chr1g0331581
rosa_laevigata RLG00000029728
rosa_multiflora Rmu_sc0008675.1_g000002
rosa_roxburghii Rroxscaffold_4G00319040
rosa_rugosa Rorug01G0094900
rosa_samantha Rh1AG118700 Rh1BG090400 Rh1CG113700 Rh1DG122900
rosa_wichuraiana Rw1G009510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 505
AccI GTMKAC 1 cut(s) 891
AccII CGCG 1 cut(s) 18
AccIII TCCGGA 1 cut(s) 1166
AciI CCGC 3 cut(s) 357, 399, 806
AclI AACGTT 1 cut(s) 994
AclWI GGATC 1 cut(s) 338
AcsI RAATTY 2 cut(s) 279, 768
AcuI CTGAAG 1 cut(s) 126
AfaI GTAC 1 cut(s) 836
AfiI CCNNNNNNNGG 1 cut(s) 755
AflIII ACRYGT 2 cut(s) 16, 35
AgsI TTSAA 5 cut(s) 89, 94, 128, 145, 238
AjiI CACGTC 1 cut(s) 38
AjnI CCWGG 2 cut(s) 133, 178
AluBI AGCT 8 cut(s) 110, 362, 569, 593, 641, 674, 938, 1087
AluI AGCT 8 cut(s) 110, 362, 569, 593, 641, 674, 938, 1087
Alw21I GWGCWC 3 cut(s) 174, 481, 1163
Alw26I GTCTC 2 cut(s) 377, 436
Alw44I GTGCAC 1 cut(s) 170
AlwI GGATC 1 cut(s) 338
Ama87I CYCGRG 1 cut(s) 513
Aor13HI TCCGGA 1 cut(s) 1166
ApaLI GTGCAC 1 cut(s) 170
ApoI RAATTY 2 cut(s) 279, 768
ArsI GACNNNNNNTTYG 2 cut(s) 36, 68
AspS9I GGNCC 2 cut(s) 193, 466
AsuC2I CCSGG 3 cut(s) 514, 515, 861
AsuHPI GGTGA 3 cut(s) 623, 724, 929
AsuII TTCGAA 1 cut(s) 455
AvaI CYCGRG 1 cut(s) 513
AvaII GGWCC 2 cut(s) 193, 466
BaeGI GKGCMC 1 cut(s) 174
BanII GRGCYC 2 cut(s) 603, 744
BarI GAAGNNNNNNTAC 2 cut(s) 794, 826
Bbv12I GWGCWC 3 cut(s) 174, 481, 1163
BccI CCATC 3 cut(s) 493, 556, 586
BceAI ACGGC 1 cut(s) 216
BcgI CGANNNNNNTGC 2 cut(s) 935, 969
BciT130I CCWGG 2 cut(s) 135, 180
BclI TGATCA 2 cut(s) 186, 714
BcnI CCSGG 3 cut(s) 514, 515, 861
BcoDI GTCTC 2 cut(s) 377, 436
BfmI CTRYAG 1 cut(s) 363
BfuAI ACCTGC 1 cut(s) 505
Bme1390I CCNGG 5 cut(s) 135, 180, 514, 515, 861
Bme18I GGWCC 2 cut(s) 193, 466
BmeT110I CYCGRG 1 cut(s) 513
BmgBI CACGTC 1 cut(s) 38
BmgT120I GGNCC 2 cut(s) 193, 466
BmiI GGNNCC 3 cut(s) 194, 230, 450
BmrFI CCNGG 5 cut(s) 135, 180, 514, 515, 861
BmrI ACTGGG 1 cut(s) 76
BmsI GCATC 1 cut(s) 613
BmuI ACTGGG 1 cut(s) 76
BpmI CTGGAG 1 cut(s) 663
Bpu14I TTCGAA 1 cut(s) 455
BpuMI CCSGG 3 cut(s) 514, 515, 861
BsaAI YACGTR 1 cut(s) 476
BsaJI CCNNGG 4 cut(s) 178, 196, 211, 513
BsaWI WCCGGW 1 cut(s) 1166
Bsc4I CCNNNNNNNGG 1 cut(s) 755
Bse1I ACTGG 2 cut(s) 82, 919
Bse3DI GCAATG 1 cut(s) 317
BseAI TCCGGA 1 cut(s) 1166
BseBI CCWGG 2 cut(s) 135, 180
BseDI CCNNGG 4 cut(s) 178, 196, 211, 513
BseGI GGATG 4 cut(s) 445, 628, 706, 769
BseLI CCNNNNNNNGG 1 cut(s) 755
BseMI GCAATG 1 cut(s) 317
BseMII CTCAG 2 cut(s) 402, 893
BseNI ACTGG 2 cut(s) 82, 919
BseRI GAGGAG 3 cut(s) 471, 564, 1153
BseSI GKGCMC 1 cut(s) 174
Bsh1236I CGCG 1 cut(s) 18
BsiHKAI GWGCWC 3 cut(s) 174, 481, 1163
BsiHKCI CYCGRG 1 cut(s) 513
BsiSI CCGG 3 cut(s) 514, 860, 1167
BslFI GGGAC 3 cut(s) 42, 317, 530
BslI CCNNNNNNNGG 1 cut(s) 755
BsmAI GTCTC 2 cut(s) 377, 436
BsmFI GGGAC 3 cut(s) 42, 317, 530
BsmI GAATGC 1 cut(s) 880
BsoBI CYCGRG 1 cut(s) 513
Bsp119I TTCGAA 1 cut(s) 455
Bsp1286I GDGCHC 5 cut(s) 174, 481, 603, 744, 1163
Bsp13I TCCGGA 1 cut(s) 1166
Bsp143I GATC 5 cut(s) 186, 343, 714, 942, 970
Bsp19I CCATGG 1 cut(s) 196
BspACI CCGC 3 cut(s) 357, 399, 806
BspCNI CTCAG 2 cut(s) 403, 894
BspEI TCCGGA 1 cut(s) 1166
BspFNI CGCG 1 cut(s) 18
BspLI GGNNCC 3 cut(s) 194, 230, 450
BspMAI CTGCAG 1 cut(s) 367
BspMI ACCTGC 1 cut(s) 505
BspPI GGATC 1 cut(s) 338
BspQI GCTCTTC 1 cut(s) 980
BspT104I TTCGAA 1 cut(s) 455
BsrDI GCAATG 1 cut(s) 317
BsrI ACTGG 2 cut(s) 82, 919
BssECI CCNNGG 4 cut(s) 178, 196, 211, 513
BssMI GATC 5 cut(s) 186, 343, 714, 942, 970
BssT1I CCWWGG 2 cut(s) 196, 211
Bst2UI CCWGG 2 cut(s) 135, 180
Bst6I CTCTTC 3 cut(s) 258, 701, 980
BstBAI YACGTR 1 cut(s) 476
BstBI TTCGAA 1 cut(s) 455
BstC8I GCNNGC 2 cut(s) 174, 1024
BstDEI CTNAG 2 cut(s) 411, 902
BstDSI CCRYGG 1 cut(s) 196
BstF5I GGATG 4 cut(s) 445, 628, 706, 769
BstFNI CGCG 1 cut(s) 18
BstKTI GATC 5 cut(s) 189, 346, 717, 945, 973
BstMAI GTCTC 2 cut(s) 377, 436
BstMBI GATC 5 cut(s) 186, 343, 714, 942, 970
BstMWI GCNNNNNNNGC 8 cut(s) 362, 365, 413, 506, 509, 647, 653, 1133
BstNI CCWGG 2 cut(s) 135, 180
BstSCI CCNGG 5 cut(s) 133, 178, 512, 513, 859
BstSFI CTRYAG 1 cut(s) 363
BstSLI GKGCMC 1 cut(s) 174
BstUI CGCG 1 cut(s) 18
BstX2I RGATCY 1 cut(s) 343
BstYI RGATCY 1 cut(s) 343
BtgI CCRYGG 1 cut(s) 196
BtrI CACGTC 1 cut(s) 38
BtsCI GGATG 4 cut(s) 445, 628, 706, 769
BtsI GCAGTG 1 cut(s) 1009
BtsIMutI CAGTG 1 cut(s) 1009
BveI ACCTGC 1 cut(s) 505
Cac8I GCNNGC 2 cut(s) 174, 1024
Cfr13I GGNCC 2 cut(s) 193, 466
Cfr9I CCCGGG 1 cut(s) 513
Csp6I GTAC 1 cut(s) 835
CviAII CATG 2 cut(s) 197, 284
CviQI GTAC 1 cut(s) 835
DdeI CTNAG 2 cut(s) 411, 902
DpnI GATC 5 cut(s) 188, 345, 716, 944, 972
DpnII GATC 5 cut(s) 186, 343, 714, 942, 970
Eam1104I CTCTTC 3 cut(s) 258, 701, 980
EarI CTCTTC 3 cut(s) 258, 701, 980
EciI GGCGGA 1 cut(s) 821
Eco130I CCWWGG 2 cut(s) 196, 211
Eco24I GRGCYC 2 cut(s) 603, 744
Eco47I GGWCC 2 cut(s) 193, 466
Eco57I CTGAAG 1 cut(s) 126
Eco88I CYCGRG 1 cut(s) 513
EcoRII CCWGG 2 cut(s) 133, 178
EcoT14I CCWWGG 2 cut(s) 196, 211
EcoT38I GRGCYC 2 cut(s) 603, 744
ErhI CCWWGG 2 cut(s) 196, 211
FaeI CATG 2 cut(s) 200, 287
FaqI GGGAC 3 cut(s) 42, 317, 530
FatI CATG 2 cut(s) 196, 283
FauNDI CATATG 1 cut(s) 224
FbaI TGATCA 2 cut(s) 186, 714
FblI GTMKAC 1 cut(s) 891
FokI GGATG 4 cut(s) 452, 635, 713, 776
FriOI GRGCYC 2 cut(s) 603, 744
GsuI CTGGAG 1 cut(s) 663
HapII CCGG 3 cut(s) 514, 860, 1167
Hin1II CATG 2 cut(s) 200, 287
HincII GTYRAC 1 cut(s) 892
HindII GTYRAC 1 cut(s) 892
HpaII CCGG 3 cut(s) 514, 860, 1167
HphI GGTGA 3 cut(s) 623, 724, 929
Hpy166II GTNNAC 2 cut(s) 172, 892
Hpy188I TCNGA 3 cut(s) 436, 903, 975
Hpy188III TCNNGA 4 cut(s) 620, 680, 827, 1167
Hpy8I GTNNAC 2 cut(s) 172, 892
HpyAV CCTTC 4 cut(s) 88, 457, 462, 796
HpyCH4IV ACGT 3 cut(s) 37, 475, 994
HpyCH4V TGCA 5 cut(s) 172, 365, 734, 1026, 1034
HpyF10VI GCNNNNNNNGC 8 cut(s) 362, 365, 413, 506, 509, 647, 653, 1133
HpyF3I CTNAG 2 cut(s) 411, 902
HpySE526I ACGT 3 cut(s) 37, 475, 994
Hsp92II CATG 2 cut(s) 200, 287
Kpn2I TCCGGA 1 cut(s) 1166
Ksp22I TGATCA 2 cut(s) 186, 714
Kzo9I GATC 5 cut(s) 186, 343, 714, 942, 970
LguI GCTCTTC 1 cut(s) 980
LmnI GCTCC 5 cut(s) 228, 484, 577, 682, 1166
LweI GCATC 1 cut(s) 613
MaeII ACGT 3 cut(s) 37, 475, 994
MalI GATC 5 cut(s) 188, 345, 716, 944, 972
MboI GATC 5 cut(s) 186, 343, 714, 942, 970
MboII GAAGA 7 cut(s) 110, 245, 718, 944, 994, 997, 1088
MflI RGATCY 1 cut(s) 343
MhlI GDGCHC 5 cut(s) 174, 481, 603, 744, 1163
MluCI AATT 6 cut(s) 89, 262, 279, 604, 768, 1110
MluI ACGCGT 1 cut(s) 16
MroI TCCGGA 1 cut(s) 1166
MseI TTAA 1 cut(s) 294
MspA1I CMGCKG 4 cut(s) 362, 401, 593, 1087
MspI CCGG 3 cut(s) 514, 860, 1167
MspR9I CCNGG 5 cut(s) 135, 180, 514, 515, 861
Mva1269I GAATGC 1 cut(s) 880
MvaI CCWGG 2 cut(s) 135, 180
MvnI CGCG 1 cut(s) 18
MwoI GCNNNNNNNGC 8 cut(s) 362, 365, 413, 506, 509, 647, 653, 1133
NciI CCSGG 3 cut(s) 514, 515, 861
NcoI CCATGG 1 cut(s) 196
NdeI CATATG 1 cut(s) 224
NdeII GATC 5 cut(s) 186, 343, 714, 942, 970
NlaIII CATG 2 cut(s) 200, 287
NlaIV GGNNCC 3 cut(s) 194, 230, 450
NspV TTCGAA 1 cut(s) 455
PciSI GCTCTTC 1 cut(s) 980
PctI GAATGC 1 cut(s) 880
Ppu21I YACGTR 1 cut(s) 476
Psp1406I AACGTT 1 cut(s) 994
Psp6I CCWGG 2 cut(s) 133, 178
PspGI CCWGG 2 cut(s) 133, 178
PspN4I GGNNCC 3 cut(s) 194, 230, 450
PspPI GGNCC 2 cut(s) 193, 466
PstI CTGCAG 1 cut(s) 367
PsuI RGATCY 1 cut(s) 343
PvuII CAGCTG 3 cut(s) 362, 593, 1087
RsaI GTAC 1 cut(s) 836
RsaNI GTAC 1 cut(s) 835
SalI GTCGAC 1 cut(s) 890
SapI GCTCTTC 1 cut(s) 980
SaqAI TTAA 1 cut(s) 294
Sau3AI GATC 5 cut(s) 186, 343, 714, 942, 970
Sau96I GGNCC 2 cut(s) 193, 466
ScrFI CCNGG 5 cut(s) 135, 180, 514, 515, 861
SduI GDGCHC 5 cut(s) 174, 481, 603, 744, 1163
SfaNI GCATC 1 cut(s) 613
SfcI CTRYAG 1 cut(s) 363
SfuI TTCGAA 1 cut(s) 455
SinI GGWCC 2 cut(s) 193, 466
SmaI CCCGGG 1 cut(s) 515
Sse9I AATT 6 cut(s) 89, 262, 279, 604, 768, 1110
SsiI CCGC 3 cut(s) 357, 399, 806
StyD4I CCNGG 5 cut(s) 133, 178, 512, 513, 859
StyI CCWWGG 2 cut(s) 196, 211
TaiI ACGT 3 cut(s) 40, 478, 997
TaqI TCGA 4 cut(s) 455, 597, 891, 945
TasI AATT 6 cut(s) 89, 262, 279, 604, 768, 1110
TauI GCSGC 1 cut(s) 359
Tru1I TTAA 1 cut(s) 294
Tru9I TTAA 1 cut(s) 294
TscAI CASTG 1 cut(s) 1009
TspDTI ATGAA 4 cut(s) 272, 780, 807, 1089
TspMI CCCGGG 1 cut(s) 513
TspRI CASTG 1 cut(s) 1009
VneI GTGCAC 1 cut(s) 170
VpaK11BI GGWCC 2 cut(s) 193, 466
XapI RAATTY 2 cut(s) 279, 768
XmaI CCCGGG 1 cut(s) 513
XmiI GTMKAC 1 cut(s) 891
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.