MD07G1061100.v1.1

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0013354
Physical Location & Seq
Forward (+)
5721771 .. 5723459
1689 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1061100.v1.1.491

Sequence Viewer

Length: 1197 bp
ATGTCTATGGGACATCGAGAGGGAGAGATGCCTATGACCCGCCCAAGACAATCCCATCGTCCCATTTATGAAGATTTCCAGCCCCACTTTGAATTGAAGGAAGAGCAGCAAGCTCACATTGTACAAGTTCATCTGCCTGGTTTTGTGAAGGAGCAGGCTAAGATTACATATATGCATAACCCTGGTGTGATTAGGGTTTATGGACAAAGACCTCTCGACAACAACAAATGGAGCCGTTTCGACCAAACATTTCCGGTTCAACATAATTGCGACGCAACTAAAATTCATGCCAAATTTAGCAATGGGATTCTTACCATTACAATACCAAAGAAAGTCATCATCACATGGACTGCAACAGCCGAACCTGCTGCAATCACGGTCAAGCCGAAGCCACACAAAGTTCAAGAGGACCCAATTCCTTCAAAGCCGAGTTTGACAACACCGGCAGGCCCTCCGACAGACACCTCTTCGGATACAAAGGGTCGGGAAGTTATTTCTCAAACAGCTGCTGCTTCTGCTCAAGGTGAACAACAAAGGGGCAAGACAAGCGCTCAGGAAGCTGCTGCTGCTGCCGCGTCGAAGGCAGAAAAGGCTACTTTCACTGCCAGAGATGAAAAAGAAATGGATGACAAGAATGGTCGTCCTCCATTAGCTCCTCCGAAAGGCACTGCAGCTGAGACAAAAGCACAAGAGGACAAGGAGGATGAGAAGAGGCTGATGCCGTTGACTAGTCCGGAAACAGGCATAGGCGAGCCCAAGTCTGAGGAGGGTGATCAGGATGGAAAGAGAGGAAAGGCTTCTGCCAAGAGTGTTTATCAAACGGGGGTTGAAAACAAGGATCAGAAAGAAGCAAAAGGGAAGGCTGATGCAGAACCGAAGAATGCTGAGAACGTTGTCAAAACTAATATACACAAGGAAAGGGAGACCGGTGAGAGAAGCAAAGAAGCAAGGGCCCTCCTTAAGATTTTAGAGAGGGAAGAGAACAAGAGCAGGGGTTTCAGTAAGCCTAAGGAGGAGGGTTCTATTGCTGCACATGTGATGGCTGCTGCAAAGGAGCGTATGAAAAATCTCGGCAACGGGATGAATGATGAAAAGAAGCAGATGTTGATCAATATGGGCGGAGCGGTTCTAGTTCTTATGGCGCTAGGGGCTTCTGCATCCTACATTTTGTGGTACTCTGGGAAAGGCAAAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

399

Amino Acids

43.7

Weight (kDa)

9.17

Isoelectric Point (pI)

40.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 32 - 112 1.5e-09 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013354)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G04890
fragaria_vesca FvH4_7g05240
malus_domestica MD02G1259500.v1.1 MD07G1061000.v1.1 MD07G1061100.v1.1
prunus_persica Prupe.2G065600_v2.0.a1 Prupe.2G065600_v2.0.a1
pyrus_communis pycom02g22090
rosa_chinensis RchiOBHm_Chr1g0331581
rosa_laevigata RLG00000029728
rosa_multiflora Rmu_sc0008675.1_g000002
rosa_roxburghii Rroxscaffold_4G00319040
rosa_rugosa Rorug01G0094900
rosa_samantha Rh1AG118700 Rh1BG090400 Rh1CG113700 Rh1DG122900
rosa_wichuraiana Rw1G009510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 373
AccBSI CCGCTC 1 cut(s) 1124
AccII CGCG 1 cut(s) 575
AccIII TCCGGA 1 cut(s) 733
AciI CCGC 4 cut(s) 40, 573, 1119, 1124
AclI AACGTT 1 cut(s) 891
AclWI GGATC 1 cut(s) 846
AcsI RAATTY 2 cut(s) 282, 293
AfaI GTAC 2 cut(s) 123, 1175
AfeI AGCGCT 1 cut(s) 550
AfiI CCNNNNNNNGG 2 cut(s) 662, 740
AflII CTTAAG 1 cut(s) 959
AflIII ACRYGT 1 cut(s) 1033
AgeI ACCGGT 1 cut(s) 926
AgsI TTSAA 6 cut(s) 92, 97, 260, 404, 423, 830
AhlI ACTAGT 1 cut(s) 728
AjnI CCWGG 2 cut(s) 136, 181
AluBI AGCT 5 cut(s) 113, 506, 560, 653, 674
AluI AGCT 5 cut(s) 113, 506, 560, 653, 674
Alw26I GTCTC 2 cut(s) 671, 917
AlwI GGATC 1 cut(s) 846
AlwNI CAGNNNCTG 1 cut(s) 509
Aor13HI TCCGGA 1 cut(s) 733
Aor51HI AGCGCT 1 cut(s) 550
AoxI GGCC 2 cut(s) 448, 951
ApaI GGGCCC 1 cut(s) 955
ApoI RAATTY 2 cut(s) 282, 293
AsiGI ACCGGT 1 cut(s) 926
Asp700I GAANNNNTTC 1 cut(s) 796
AspLEI GCGC 2 cut(s) 551, 1144
AspS9I GGNCC 4 cut(s) 409, 449, 951, 952
AsuHPI GGTGA 3 cut(s) 536, 782, 941
AvaII GGWCC 1 cut(s) 409
AxyI CCTNAGG 1 cut(s) 1008
BaeGI GKGCMC 1 cut(s) 955
BanII GRGCYC 2 cut(s) 756, 955
BccI CCATC 3 cut(s) 63, 773, 1033
BceAI ACGGC 2 cut(s) 219, 706
BcgI CGANNNNNNTGC 2 cut(s) 350, 384
BciT130I CCWGG 2 cut(s) 138, 183
BciVI GTATCC 1 cut(s) 466
BclI TGATCA 2 cut(s) 772, 1107
BcoDI GTCTC 2 cut(s) 671, 917
BcuI ACTAGT 1 cut(s) 728
BfaI CTAG 3 cut(s) 729, 1130, 1145
BfmI CTRYAG 1 cut(s) 669
BfoI RGCGCY 2 cut(s) 552, 1145
BfrI CTTAAG 1 cut(s) 959
BfuAI ACCTGC 1 cut(s) 373
BfuI GTATCC 1 cut(s) 466
Bme1390I CCNGG 2 cut(s) 138, 183
Bme18I GGWCC 1 cut(s) 409
BmgT120I GGNCC 4 cut(s) 409, 449, 951, 952
BmiI GGNNCC 3 cut(s) 233, 411, 953
BmrFI CCNGG 2 cut(s) 138, 183
BmsI GCATC 4 cut(s) 18, 708, 856, 1166
Bpu10I CCTNAGC 1 cut(s) 552
BpuEI CTTGAG 1 cut(s) 504
BsaBI GATNNNNATC 1 cut(s) 1106
BsaI GGTCTC 1 cut(s) 917
BsaJI CCNNGG 1 cut(s) 181
BsaWI WCCGGW 3 cut(s) 253, 733, 926
Bsc4I CCNNNNNNNGG 2 cut(s) 662, 740
Bse118I RCCGGY 2 cut(s) 442, 926
Bse21I CCTNAGG 1 cut(s) 1008
Bse3DI GCAATG 1 cut(s) 307
Bse8I GATNNNNATC 1 cut(s) 1106
BseAI TCCGGA 1 cut(s) 733
BseBI CCWGG 2 cut(s) 138, 183
BseDI CCNNGG 1 cut(s) 181
BseGI GGATG 5 cut(s) 631, 709, 784, 1086, 1157
BseJI GATNNNNATC 1 cut(s) 1106
BseLI CCNNNNNNNGG 2 cut(s) 662, 740
BseMI GCAATG 1 cut(s) 307
BseMII CTCAG 4 cut(s) 566, 666, 753, 876
BseRI GAGGAG 3 cut(s) 645, 779, 1028
BseSI GKGCMC 1 cut(s) 955
BsgI GTGCAG 1 cut(s) 1014
Bsh1236I CGCG 1 cut(s) 575
BshFI GGCC 2 cut(s) 450, 953
BshTI ACCGGT 1 cut(s) 926
BsiSI CCGG 4 cut(s) 254, 443, 734, 927
BslFI GGGAC 2 cut(s) 24, 45
BslI CCNNNNNNNGG 2 cut(s) 662, 740
BsmAI GTCTC 2 cut(s) 671, 917
BsmFI GGGAC 2 cut(s) 24, 45
BsmI GAATGC 1 cut(s) 886
BsnI GGCC 2 cut(s) 450, 953
Bso31I GGTCTC 1 cut(s) 917
Bsp120I GGGCCC 1 cut(s) 951
Bsp1286I GDGCHC 2 cut(s) 756, 955
Bsp13I TCCGGA 1 cut(s) 733
Bsp1407I TGTACA 1 cut(s) 121
Bsp143I GATC 3 cut(s) 772, 838, 1107
BspACI CCGC 4 cut(s) 40, 573, 1119, 1124
BspANI GGCC 2 cut(s) 450, 953
BspCNI CTCAG 4 cut(s) 565, 667, 754, 877
BspEI TCCGGA 1 cut(s) 733
BspFNI CGCG 1 cut(s) 575
BspLI GGNNCC 3 cut(s) 233, 411, 953
BspMAI CTGCAG 1 cut(s) 673
BspMI ACCTGC 1 cut(s) 373
BspPI GGATC 1 cut(s) 846
BspQI GCTCTTC 1 cut(s) 96
BspTI CTTAAG 1 cut(s) 959
BspTNI GGTCTC 1 cut(s) 917
BsrBI CCGCTC 1 cut(s) 1124
BsrDI GCAATG 1 cut(s) 307
BsrFI RCCGGY 2 cut(s) 442, 926
BsrGI TGTACA 1 cut(s) 121
BssAI RCCGGY 2 cut(s) 442, 926
BssECI CCNNGG 1 cut(s) 181
BssMI GATC 3 cut(s) 772, 838, 1107
Bst2UI CCWGG 2 cut(s) 138, 183
Bst4CI ACNGT 1 cut(s) 379
Bst6I CTCTTC 4 cut(s) 96, 472, 704, 972
BstAFI CTTAAG 1 cut(s) 959
BstAUI TGTACA 1 cut(s) 121
BstC8I GCNNGC 4 cut(s) 111, 156, 448, 752
BstDEI CTNAG 6 cut(s) 159, 552, 675, 762, 885, 1008
BstENI CCTNNNNNAGG 1 cut(s) 660
BstF5I GGATG 5 cut(s) 631, 709, 784, 1086, 1157
BstFNI CGCG 1 cut(s) 575
BstH2I RGCGCY 2 cut(s) 552, 1145
BstHHI GCGC 2 cut(s) 551, 1144
BstKTI GATC 3 cut(s) 775, 841, 1110
BstMAI GTCTC 2 cut(s) 671, 917
BstMBI GATC 3 cut(s) 772, 838, 1107
BstNI CCWGG 2 cut(s) 138, 183
BstNSI RCATGY 1 cut(s) 1037
BstSCI CCNGG 2 cut(s) 136, 181
BstSFI CTRYAG 1 cut(s) 669
BstSLI GKGCMC 1 cut(s) 955
BstUI CGCG 1 cut(s) 575
Bsu36I CCTNAGG 1 cut(s) 1008
BsuI GTATCC 1 cut(s) 466
BsuRI GGCC 2 cut(s) 450, 953
BtsCI GGATG 5 cut(s) 631, 709, 784, 1086, 1157
BtsI GCAGTG 2 cut(s) 600, 666
BtsIMutI CAGTG 2 cut(s) 600, 666
BveI ACCTGC 1 cut(s) 373
Cac8I GCNNGC 4 cut(s) 111, 156, 448, 752
CaiI CAGNNNCTG 1 cut(s) 509
CfoI GCGC 2 cut(s) 551, 1144
Cfr10I RCCGGY 2 cut(s) 442, 926
Cfr13I GGNCC 4 cut(s) 409, 449, 951, 952
CseI GACGC 2 cut(s) 281, 564
Csp6I GTAC 2 cut(s) 122, 1174
CspAI ACCGGT 1 cut(s) 926
CviAII CATG 3 cut(s) 287, 345, 1034
CviQI GTAC 2 cut(s) 122, 1174
DdeI CTNAG 6 cut(s) 159, 552, 675, 762, 885, 1008
DpnI GATC 3 cut(s) 774, 840, 1109
DpnII GATC 3 cut(s) 772, 838, 1107
Eam1104I CTCTTC 4 cut(s) 96, 472, 704, 972
EarI CTCTTC 4 cut(s) 96, 472, 704, 972
EciI GGCGGA 1 cut(s) 1134
Eco24I GRGCYC 2 cut(s) 756, 955
Eco31I GGTCTC 1 cut(s) 917
Eco47I GGWCC 1 cut(s) 409
Eco47III AGCGCT 1 cut(s) 550
Eco81I CCTNAGG 1 cut(s) 1008
EcoNI CCTNNNNNAGG 1 cut(s) 660
EcoO109I RGGNCCY 4 cut(s) 409, 449, 951, 952
EcoRII CCWGG 2 cut(s) 136, 181
EcoT22I ATGCAT 1 cut(s) 177
EcoT38I GRGCYC 2 cut(s) 756, 955
FaeI CATG 3 cut(s) 290, 348, 1037
FaqI GGGAC 2 cut(s) 24, 45
FatI CATG 3 cut(s) 286, 344, 1033
FauI CCCGC 1 cut(s) 47
FbaI TGATCA 2 cut(s) 772, 1107
FokI GGATG 5 cut(s) 638, 716, 791, 1093, 1144
FriOI GRGCYC 2 cut(s) 756, 955
FspBI CTAG 3 cut(s) 729, 1130, 1145
GlaI GCGC 2 cut(s) 550, 1143
HaeII RGCGCY 2 cut(s) 552, 1145
HaeIII GGCC 2 cut(s) 450, 953
HapII CCGG 4 cut(s) 254, 443, 734, 927
HgaI GACGC 2 cut(s) 281, 564
HhaI GCGC 2 cut(s) 551, 1144
Hin1II CATG 3 cut(s) 290, 348, 1037
Hin6I GCGC 2 cut(s) 549, 1142
HinP1I GCGC 2 cut(s) 549, 1142
HincII GTYRAC 1 cut(s) 726
HindII GTYRAC 1 cut(s) 726
HinfI GANTC 1 cut(s) 307
HpaII CCGG 4 cut(s) 254, 443, 734, 927
HphI GGTGA 3 cut(s) 536, 782, 941
Hpy166II GTNNAC 2 cut(s) 527, 726
Hpy188I TCNGA 5 cut(s) 456, 472, 660, 763, 843
Hpy188III TCNNGA 7 cut(s) 17, 215, 404, 485, 554, 734, 776
Hpy8I GTNNAC 2 cut(s) 527, 726
Hpy99I CGWCG 2 cut(s) 275, 580
HpyAV CCTTC 5 cut(s) 91, 142, 429, 574, 853
HpyCH4III ACNGT 1 cut(s) 379
HpyCH4IV ACGT 1 cut(s) 891
HpyCH4V TGCA 8 cut(s) 175, 353, 371, 671, 869, 1031, 1049, 1157
HpyF3I CTNAG 6 cut(s) 159, 552, 675, 762, 885, 1008
HpySE526I ACGT 1 cut(s) 891
Hsp92II CATG 3 cut(s) 290, 348, 1037
HspAI GCGC 2 cut(s) 549, 1142
Kpn2I TCCGGA 1 cut(s) 733
Ksp22I TGATCA 2 cut(s) 772, 1107
Kzo9I GATC 3 cut(s) 772, 838, 1107
LguI GCTCTTC 1 cut(s) 96
LmnI GCTCC 5 cut(s) 151, 231, 658, 1054, 1121
LweI GCATC 4 cut(s) 18, 708, 856, 1166
MaeI CTAG 3 cut(s) 729, 1130, 1145
MaeII ACGT 1 cut(s) 891
MalI GATC 3 cut(s) 774, 840, 1109
MbiI CCGCTC 1 cut(s) 1124
MboI GATC 3 cut(s) 772, 838, 1107
MboII GAAGA 6 cut(s) 83, 113, 459, 721, 889, 989
MhlI GDGCHC 2 cut(s) 756, 955
MluCI AATT 5 cut(s) 92, 265, 282, 293, 414
MmeI TCCRAC 1 cut(s) 479
Mph1103I ATGCAT 1 cut(s) 177
MroI TCCGGA 1 cut(s) 733
MroXI GAANNNNTTC 1 cut(s) 796
MseI TTAA 1 cut(s) 960
MspA1I CMGCKG 2 cut(s) 506, 674
MspCI CTTAAG 1 cut(s) 959
MspI CCGG 4 cut(s) 254, 443, 734, 927
MspR9I CCNGG 2 cut(s) 138, 183
Mva1269I GAATGC 1 cut(s) 886
MvaI CCWGG 2 cut(s) 138, 183
MvnI CGCG 1 cut(s) 575
NdeII GATC 3 cut(s) 772, 838, 1107
NlaIII CATG 3 cut(s) 290, 348, 1037
NlaIV GGNNCC 3 cut(s) 233, 411, 953
NmeAIII GCCGAG 2 cut(s) 453, 1050
NsiI ATGCAT 1 cut(s) 177
NspI RCATGY 1 cut(s) 1037
PciI ACATGT 1 cut(s) 1033
PciSI GCTCTTC 1 cut(s) 96
PcsI WCGNNNNNNNCGW 1 cut(s) 383
PctI GAATGC 1 cut(s) 886
PdmI GAANNNNTTC 1 cut(s) 796
PfeI GAWTC 1 cut(s) 307
PinAI ACCGGT 1 cut(s) 926
PpuMI RGGWCCY 1 cut(s) 409
PscI ACATGT 1 cut(s) 1033
Psp1406I AACGTT 1 cut(s) 891
Psp5II RGGWCCY 1 cut(s) 409
Psp6I CCWGG 2 cut(s) 136, 181
PspGI CCWGG 2 cut(s) 136, 181
PspN4I GGNNCC 3 cut(s) 233, 411, 953
PspOMI GGGCCC 1 cut(s) 951
PspPI GGNCC 4 cut(s) 409, 449, 951, 952
PspPPI RGGWCCY 1 cut(s) 409
PstI CTGCAG 1 cut(s) 673
PstNI CAGNNNCTG 1 cut(s) 509
PvuII CAGCTG 2 cut(s) 506, 674
RsaI GTAC 2 cut(s) 123, 1175
RsaNI GTAC 2 cut(s) 122, 1174
SapI GCTCTTC 1 cut(s) 96
SaqAI TTAA 1 cut(s) 960
Sau3AI GATC 3 cut(s) 772, 838, 1107
Sau96I GGNCC 4 cut(s) 409, 449, 951, 952
ScrFI CCNGG 2 cut(s) 138, 183
SduI GDGCHC 2 cut(s) 756, 955
SfaNI GCATC 4 cut(s) 18, 708, 856, 1166
SfcI CTRYAG 1 cut(s) 669
SinI GGWCC 1 cut(s) 409
SmlI CTYRAG 2 cut(s) 519, 959
SmoI CTYRAG 2 cut(s) 519, 959
SpeI ACTAGT 1 cut(s) 728
Sse9I AATT 5 cut(s) 92, 265, 282, 293, 414
SsiI CCGC 4 cut(s) 40, 573, 1119, 1124
SspMI CTAG 3 cut(s) 729, 1130, 1145
StyD4I CCNGG 2 cut(s) 136, 181
TaaI ACNGT 1 cut(s) 379
TaiI ACGT 1 cut(s) 894
TaqI TCGA 4 cut(s) 16, 216, 240, 578
TasI AATT 5 cut(s) 92, 265, 282, 293, 414
TatI WGTACW 1 cut(s) 121
TauI GCSGC 1 cut(s) 575
TfiI GAWTC 1 cut(s) 307
Tru1I TTAA 1 cut(s) 960
Tru9I TTAA 1 cut(s) 960
TscAI CASTG 2 cut(s) 607, 673
TspDTI ATGAA 7 cut(s) 84, 119, 275, 627, 1076, 1097, 1104
TspRI CASTG 2 cut(s) 607, 673
Vha464I CTTAAG 1 cut(s) 959
VpaK11BI GGWCC 1 cut(s) 409
XagI CCTNNNNNAGG 1 cut(s) 660
XapI RAATTY 2 cut(s) 282, 293
XceI RCATGY 1 cut(s) 1037
XmnI GAANNNNTTC 1 cut(s) 796
XspI CTAG 3 cut(s) 729, 1130, 1145
Zsp2I ATGCAT 1 cut(s) 177
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.