MD02G1259500.v1.1

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0013354
Physical Location & Seq
Reverse (-)
31287370 .. 31288929
1560 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1259500.v1.1.491

Sequence Viewer

Length: 1092 bp
ATGGTTATGCGACGTGGTCCAATTGGTGGAAGCTCTGGTTCTAATACCCGACCAAGACAAACAAATCGTCCCACTTATGAAGATTTCCAGCCCCGCTTTGAATTGAAGGAAGAGCAGGAAGCTCACAGCGTACTTGTTCATCTACCTGGGTTTGTGAAGGAGCAGGTGAGGATTACTATTGAGCATAACCCTAATGTGATTAGGGTTCATGGACAAAGACCTCTGGGCAACAACAAATATAGCCGTTTCAACCAAACATTTCCGATTCATGAAAATTGCGACGTAAGTAAAATTGATGGCAAATTTAACGATGGGATTCTTACCATCACAATACCAAAGCCAGTCATCACACAGACAGCAGCAACCAAACCAGTCCAAGAGCCACCACCGAAAACTGCTGCAACCGCTCCCAAGCCGATGCCACAGAAAGTTAAAGAGGACCCAATTCCTTCAAAGCCGAGTTTGACAACACCGGCCGCTGCCGCGTCAAAGGCAGAAAAGGCTACTTTCACTGCCAGAGATGAAAAAGAAAAGGATGACAAGAATGGTGGTCGTCCATTAGGCCCTCCGAAAGGCACTGCAGCCGAGACAAAAGCACAGAAGGATAAGGAGGACGCGGTTCTTCCAAAACCAGTTTCATCAACCCAGAGTGACAAACAAAGGGATGAGAAGAGGCTGTTACTTTTGACTAGTCCGGAAACAGGCATCGGCATGCCCAAGTCTCAAGAGCATGGTCATGATGGGAAGAGGGGAAAGGCTTCTGGGGTTGAAAACAAGGATCAGAGAGAAGCAAATGGGAAGCCTGATGCAGAACCGAAGAATGATGAGAAACTTGTCAAAATTACTCTACGTAAGGAAACGGAGACCGGTGAGAGAAGCAAAGAAGCAAGGGGTCTCGGTAAGCCTAAAGAGGACTCCGTGGCTGCAAATGCGATGGCTGGTGCAAAGCAGCATATGAAAAACCTCGGGAACGGGATGAGCGATGAAGAGAAGAAGATGTTGATCAATATGGGCGCGGCAGTTCTAGTTCTTCTGGCACTAGGGGCTTCTGCATCCTACAATATCTGGTCCTCTGCAACAGGCAAAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

364

Amino Acids

39.47

Weight (kDa)

9.53

Isoelectric Point (pI)

38.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 35 - 120 4.5e-11 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013354)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G04890
fragaria_vesca FvH4_7g05240
malus_domestica MD02G1259500.v1.1 MD07G1061000.v1.1 MD07G1061100.v1.1
prunus_persica Prupe.2G065600_v2.0.a1 Prupe.2G065600_v2.0.a1
pyrus_communis pycom02g22090
rosa_chinensis RchiOBHm_Chr1g0331581
rosa_laevigata RLG00000029728
rosa_multiflora Rmu_sc0008675.1_g000002
rosa_roxburghii Rroxscaffold_4G00319040
rosa_rugosa Rorug01G0094900
rosa_samantha Rh1AG118700 Rh1BG090400 Rh1CG113700 Rh1DG122900
rosa_wichuraiana Rw1G009510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 154
Acc36I ACCTGC 1 cut(s) 154
AccB7I CCANNNNNTGG 1 cut(s) 26
AccBSI CCGCTC 1 cut(s) 407
AccII CGCG 3 cut(s) 485, 617, 1016
AccIII TCCGGA 1 cut(s) 694
AciI CCGC 6 cut(s) 94, 405, 477, 483, 617, 1016
AclWI GGATC 1 cut(s) 786
AcoI YGGCCR 1 cut(s) 474
AcsI RAATTY 1 cut(s) 302
AfaI GTAC 1 cut(s) 132
AfiI CCNNNNNNNGG 3 cut(s) 26, 572, 701
AgeI ACCGGT 1 cut(s) 866
AgsI TTSAA 5 cut(s) 101, 106, 250, 453, 770
AhlI ACTAGT 1 cut(s) 689
AjiI CACGTC 1 cut(s) 14
AjnI CCWGG 1 cut(s) 145
AjuI GAANNNNNNNTTGG 2 cut(s) 619, 651
AluBI AGCT 2 cut(s) 33, 122
AluI AGCT 2 cut(s) 33, 122
Alw26I GTCTC 4 cut(s) 581, 726, 857, 899
AlwI GGATC 1 cut(s) 786
Ama87I CYCGRG 1 cut(s) 965
Aor13HI TCCGGA 1 cut(s) 694
AoxI GGCC 2 cut(s) 474, 562
ApeKI GCWGC 6 cut(s) 359, 398, 479, 581, 923, 949
ApoI RAATTY 1 cut(s) 302
ArsI GACNNNNNNTTYG 2 cut(s) 52, 84
AsiGI ACCGGT 1 cut(s) 866
Asp700I GAANNNNTTC 1 cut(s) 757
AspLEI GCGC 1 cut(s) 1016
AspS9I GGNCC 4 cut(s) 17, 439, 563, 1068
AsuHPI GGTGA 2 cut(s) 178, 881
AvaI CYCGRG 1 cut(s) 965
AvaII GGWCC 3 cut(s) 17, 439, 1068
BbvI GCAGC 6 cut(s) 371, 385, 466, 593, 910, 961
BccI CCATC 5 cut(s) 290, 305, 332, 734, 928
BceAI ACGGC 1 cut(s) 228
BciT130I CCWGG 1 cut(s) 147
BclI TGATCA 1 cut(s) 1002
BcoDI GTCTC 4 cut(s) 581, 726, 857, 899
BcuI ACTAGT 1 cut(s) 689
BfaI CTAG 3 cut(s) 690, 1025, 1040
BfmI CTRYAG 1 cut(s) 579
BfuAI ACCTGC 1 cut(s) 154
BisI GCNGC 9 cut(s) 360, 399, 477, 480, 483, 582, 924, 950, 1017
BlsI GCNGC 9 cut(s) 361, 400, 478, 481, 484, 583, 925, 951, 1018
Bme1390I CCNGG 1 cut(s) 147
Bme18I GGWCC 3 cut(s) 17, 439, 1068
BmeT110I CYCGRG 1 cut(s) 965
BmgBI CACGTC 1 cut(s) 14
BmgT120I GGNCC 4 cut(s) 17, 439, 563, 1068
BmiI GGNNCC 1 cut(s) 441
BmrFI CCNGG 1 cut(s) 147
BmsI GCATC 4 cut(s) 408, 714, 796, 1061
BpuEI CTTGAG 1 cut(s) 708
BsaAI YACGTR 1 cut(s) 851
BsaBI GATNNNNATC 1 cut(s) 1001
BsaI GGTCTC 2 cut(s) 857, 899
BsaJI CCNNGG 3 cut(s) 146, 918, 964
BsaWI WCCGGW 2 cut(s) 694, 866
Bsc4I CCNNNNNNNGG 3 cut(s) 26, 572, 701
Bse118I RCCGGY 2 cut(s) 472, 866
Bse1I ACTGG 3 cut(s) 341, 371, 632
Bse8I GATNNNNATC 1 cut(s) 1001
BseAI TCCGGA 1 cut(s) 694
BseBI CCWGG 1 cut(s) 147
BseDI CCNNGG 3 cut(s) 146, 918, 964
BseGI GGATG 4 cut(s) 541, 670, 981, 1052
BseJI GATNNNNATC 1 cut(s) 1001
BseLI CCNNNNNNNGG 3 cut(s) 26, 572, 701
BseNI ACTGG 3 cut(s) 341, 371, 632
BseX3I CGGCCG 1 cut(s) 474
BseXI GCAGC 6 cut(s) 371, 385, 466, 593, 910, 961
Bsh1236I CGCG 3 cut(s) 485, 617, 1016
Bsh1285I CGRYCG 1 cut(s) 477
BshFI GGCC 2 cut(s) 476, 564
BshTI ACCGGT 1 cut(s) 866
BsiEI CGRYCG 1 cut(s) 477
BsiHKCI CYCGRG 1 cut(s) 965
BsiSI CCGG 3 cut(s) 473, 695, 867
BslFI GGGAC 1 cut(s) 54
BslI CCNNNNNNNGG 3 cut(s) 26, 572, 701
BsmAI GTCTC 4 cut(s) 581, 726, 857, 899
BsmFI GGGAC 1 cut(s) 54
BsnI GGCC 2 cut(s) 476, 564
Bso31I GGTCTC 2 cut(s) 857, 899
BsoBI CYCGRG 1 cut(s) 965
Bsp13I TCCGGA 1 cut(s) 694
Bsp143I GATC 2 cut(s) 778, 1002
BspACI CCGC 6 cut(s) 94, 405, 477, 483, 617, 1016
BspANI GGCC 2 cut(s) 476, 564
BspEI TCCGGA 1 cut(s) 694
BspFNI CGCG 3 cut(s) 485, 617, 1016
BspHI TCATGA 2 cut(s) 268, 736
BspLI GGNNCC 1 cut(s) 441
BspMAI CTGCAG 1 cut(s) 583
BspMI ACCTGC 1 cut(s) 154
BspPI GGATC 1 cut(s) 786
BspQI GCTCTTC 1 cut(s) 105
BspTNI GGTCTC 2 cut(s) 857, 899
BsrBI CCGCTC 1 cut(s) 407
BsrFI RCCGGY 2 cut(s) 472, 866
BsrI ACTGG 3 cut(s) 341, 371, 632
BssAI RCCGGY 2 cut(s) 472, 866
BssECI CCNNGG 3 cut(s) 146, 918, 964
BssMI GATC 2 cut(s) 778, 1002
Bst2UI CCWGG 1 cut(s) 147
Bst6I CTCTTC 4 cut(s) 105, 665, 740, 981
BstBAI YACGTR 1 cut(s) 851
BstC8I GCNNGC 1 cut(s) 713
BstDSI CCRYGG 1 cut(s) 918
BstENI CCTNNNNNAGG 1 cut(s) 570
BstF5I GGATG 4 cut(s) 541, 670, 981, 1052
BstFNI CGCG 3 cut(s) 485, 617, 1016
BstHHI GCGC 1 cut(s) 1016
BstKTI GATC 2 cut(s) 781, 1005
BstMAI GTCTC 4 cut(s) 581, 726, 857, 899
BstMBI GATC 2 cut(s) 778, 1002
BstMCI CGRYCG 1 cut(s) 477
BstMWI GCNNNNNNNGC 6 cut(s) 404, 482, 491, 500, 929, 1043
BstNI CCWGG 1 cut(s) 147
BstNSI RCATGY 1 cut(s) 715
BstSCI CCNGG 1 cut(s) 145
BstSFI CTRYAG 1 cut(s) 579
BstSNI TACGTA 1 cut(s) 851
BstUI CGCG 3 cut(s) 485, 617, 1016
BstV1I GCAGC 6 cut(s) 371, 385, 466, 593, 910, 961
BstZI CGGCCG 1 cut(s) 474
BsuRI GGCC 2 cut(s) 476, 564
BtgI CCRYGG 1 cut(s) 918
BtgZI GCGATG 2 cut(s) 947, 996
BtrI CACGTC 1 cut(s) 14
BtsCI GGATG 4 cut(s) 541, 670, 981, 1052
BtsI GCAGTG 2 cut(s) 510, 576
BtsIMutI CAGTG 2 cut(s) 510, 576
BveI ACCTGC 1 cut(s) 154
Cac8I GCNNGC 1 cut(s) 713
CciI TCATGA 2 cut(s) 268, 736
CfoI GCGC 1 cut(s) 1016
Cfr10I RCCGGY 2 cut(s) 472, 866
Cfr13I GGNCC 4 cut(s) 17, 439, 563, 1068
CseI GACGC 2 cut(s) 474, 623
Csp6I GTAC 1 cut(s) 131
CspAI ACCGGT 1 cut(s) 866
CspCI CAANNNNNGTGG 2 cut(s) 529, 564
CviAII CATG 5 cut(s) 209, 269, 712, 731, 737
CviQI GTAC 1 cut(s) 131
DpnI GATC 2 cut(s) 780, 1004
DpnII GATC 2 cut(s) 778, 1002
EaeI YGGCCR 1 cut(s) 474
EagI CGGCCG 1 cut(s) 474
Eam1104I CTCTTC 4 cut(s) 105, 665, 740, 981
EarI CTCTTC 4 cut(s) 105, 665, 740, 981
EclXI CGGCCG 1 cut(s) 474
Eco105I TACGTA 1 cut(s) 851
Eco31I GGTCTC 2 cut(s) 857, 899
Eco47I GGWCC 3 cut(s) 17, 439, 1068
Eco52I CGGCCG 1 cut(s) 474
Eco88I CYCGRG 1 cut(s) 965
EcoNI CCTNNNNNAGG 1 cut(s) 570
EcoO109I RGGNCCY 2 cut(s) 439, 563
EcoRII CCWGG 1 cut(s) 145
FaeI CATG 5 cut(s) 212, 272, 715, 734, 740
FaqI GGGAC 1 cut(s) 54
FatI CATG 5 cut(s) 208, 268, 711, 730, 736
FauI CCCGC 1 cut(s) 101
FauNDI CATATG 1 cut(s) 954
FbaI TGATCA 1 cut(s) 1002
Fnu4HI GCNGC 9 cut(s) 360, 399, 477, 480, 483, 582, 924, 950, 1017
FokI GGATG 4 cut(s) 548, 677, 988, 1039
Fsp4HI GCNGC 9 cut(s) 360, 399, 477, 480, 483, 582, 924, 950, 1017
FspBI CTAG 3 cut(s) 690, 1025, 1040
GlaI GCGC 1 cut(s) 1015
GluI GCNGC 9 cut(s) 360, 399, 477, 480, 483, 582, 924, 950, 1017
HaeIII GGCC 2 cut(s) 476, 564
HapII CCGG 3 cut(s) 473, 695, 867
HgaI GACGC 2 cut(s) 474, 623
HhaI GCGC 1 cut(s) 1016
Hin1II CATG 5 cut(s) 212, 272, 715, 734, 740
Hin6I GCGC 1 cut(s) 1014
HinP1I GCGC 1 cut(s) 1014
HinfI GANTC 3 cut(s) 265, 316, 914
HpaII CCGG 3 cut(s) 473, 695, 867
HphI GGTGA 2 cut(s) 178, 881
Hpy188I TCNGA 3 cut(s) 264, 570, 783
Hpy188III TCNNGA 5 cut(s) 269, 695, 725, 737, 967
Hpy99I CGWCG 2 cut(s) 15, 284
HpyAV CCTTC 4 cut(s) 100, 151, 459, 595
HpyCH4IV ACGT 3 cut(s) 13, 282, 850
HpyCH4V TGCA 7 cut(s) 401, 581, 809, 926, 944, 1052, 1076
HpyF10VI GCNNNNNNNGC 6 cut(s) 404, 482, 491, 500, 929, 1043
HpySE526I ACGT 3 cut(s) 13, 282, 850
Hsp92II CATG 5 cut(s) 212, 272, 715, 734, 740
HspAI GCGC 1 cut(s) 1014
Kpn2I TCCGGA 1 cut(s) 694
Ksp22I TGATCA 1 cut(s) 1002
Kzo9I GATC 2 cut(s) 778, 1002
LguI GCTCTTC 1 cut(s) 105
LmnI GCTCC 2 cut(s) 160, 412
Lsp1109I GCAGC 6 cut(s) 371, 385, 466, 593, 910, 961
LweI GCATC 4 cut(s) 408, 714, 796, 1061
MaeI CTAG 3 cut(s) 690, 1025, 1040
MaeII ACGT 3 cut(s) 13, 282, 850
MaeIII GTNAC 2 cut(s) 650, 678
MalI GATC 2 cut(s) 780, 1004
MbiI CCGCTC 1 cut(s) 407
MboI GATC 2 cut(s) 778, 1002
MfeI CAATTG 1 cut(s) 21
MluCI AATT 7 cut(s) 21, 101, 274, 291, 302, 444, 840
MlyI GAGTC 1 cut(s) 908
MroI TCCGGA 1 cut(s) 694
MroXI GAANNNNTTC 1 cut(s) 757
MseI TTAA 2 cut(s) 306, 432
MslI CAYNNNNRTG 2 cut(s) 710, 735
MspA1I CMGCKG 1 cut(s) 479
MspI CCGG 3 cut(s) 473, 695, 867
MspR9I CCNGG 1 cut(s) 147
MunI CAATTG 1 cut(s) 21
MvaI CCWGG 1 cut(s) 147
MvnI CGCG 3 cut(s) 485, 617, 1016
MwoI GCNNNNNNNGC 6 cut(s) 404, 482, 491, 500, 929, 1043
NdeI CATATG 1 cut(s) 954
NdeII GATC 2 cut(s) 778, 1002
NlaIII CATG 5 cut(s) 212, 272, 715, 734, 740
NlaIV GGNNCC 1 cut(s) 441
NmeAIII GCCGAG 2 cut(s) 483, 610
NmuCI GTSAC 1 cut(s) 650
NspI RCATGY 1 cut(s) 715
PaeI GCATGC 1 cut(s) 715
PagI TCATGA 2 cut(s) 268, 736
PaqCI CACCTGC 1 cut(s) 154
PciSI GCTCTTC 1 cut(s) 105
PcsI WCGNNNNNNNCGW 1 cut(s) 978
PdmI GAANNNNTTC 1 cut(s) 757
PfeI GAWTC 2 cut(s) 265, 316
PflFI GACNNNGTC 1 cut(s) 15
PflMI CCANNNNNTGG 1 cut(s) 26
PinAI ACCGGT 1 cut(s) 866
PkrI GCNGC 9 cut(s) 361, 400, 478, 481, 484, 583, 925, 951, 1018
PleI GAGTC 1 cut(s) 908
PpsI GAGTC 1 cut(s) 908
Ppu21I YACGTR 1 cut(s) 851
PpuMI RGGWCCY 1 cut(s) 439
Psp5II RGGWCCY 1 cut(s) 439
Psp6I CCWGG 1 cut(s) 145
PspGI CCWGG 1 cut(s) 145
PspN4I GGNNCC 1 cut(s) 441
PspPI GGNCC 4 cut(s) 17, 439, 563, 1068
PspPPI RGGWCCY 1 cut(s) 439
PstI CTGCAG 1 cut(s) 583
PsyI GACNNNGTC 1 cut(s) 15
RsaI GTAC 1 cut(s) 132
RsaNI GTAC 1 cut(s) 131
RseI CAYNNNNRTG 2 cut(s) 710, 735
SapI GCTCTTC 1 cut(s) 105
SaqAI TTAA 2 cut(s) 306, 432
SatI GCNGC 9 cut(s) 360, 399, 477, 480, 483, 582, 924, 950, 1017
Sau3AI GATC 2 cut(s) 778, 1002
Sau96I GGNCC 4 cut(s) 17, 439, 563, 1068
SchI GAGTC 1 cut(s) 908
ScrFI CCNGG 1 cut(s) 147
SetI ASST 9 cut(s) 16, 35, 124, 148, 168, 223, 285, 853, 966
SfaNI GCATC 4 cut(s) 408, 714, 796, 1061
SfcI CTRYAG 1 cut(s) 579
SinI GGWCC 3 cut(s) 17, 439, 1068
SmiMI CAYNNNNRTG 2 cut(s) 710, 735
SmlI CTYRAG 1 cut(s) 723
SmoI CTYRAG 1 cut(s) 723
SnaBI TACGTA 1 cut(s) 851
SpeI ACTAGT 1 cut(s) 689
SphI GCATGC 1 cut(s) 715
Sse9I AATT 7 cut(s) 21, 101, 274, 291, 302, 444, 840
SsiI CCGC 6 cut(s) 94, 405, 477, 483, 617, 1016
SspMI CTAG 3 cut(s) 690, 1025, 1040
StyD4I CCNGG 1 cut(s) 145
TaiI ACGT 3 cut(s) 16, 285, 853
TasI AATT 7 cut(s) 21, 101, 274, 291, 302, 444, 840
TauI GCSGC 3 cut(s) 479, 485, 1019
TfiI GAWTC 2 cut(s) 265, 316
Tru1I TTAA 2 cut(s) 306, 432
Tru9I TTAA 2 cut(s) 306, 432
TscAI CASTG 2 cut(s) 517, 583
TseFI GTSAC 1 cut(s) 650
TseI GCWGC 6 cut(s) 359, 398, 479, 581, 923, 949
Tsp45I GTSAC 1 cut(s) 650
TspDTI ATGAA 9 cut(s) 93, 128, 197, 257, 285, 537, 627, 971, 999
TspGWI ACGGA 2 cut(s) 875, 907
TspRI CASTG 2 cut(s) 517, 583
Tth111I GACNNNGTC 1 cut(s) 15
Van91I CCANNNNNTGG 1 cut(s) 26
VpaK11BI GGWCC 3 cut(s) 17, 439, 1068
XagI CCTNNNNNAGG 1 cut(s) 570
XapI RAATTY 1 cut(s) 302
XceI RCATGY 1 cut(s) 715
XmnI GAANNNNTTC 1 cut(s) 757
XspI CTAG 3 cut(s) 690, 1025, 1040
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.