pycom02g22090

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0013354
Physical Location & Seq
Reverse (-)
20228769 .. 20230236
1468 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g22090.2

Sequence Viewer

Length: 1107 bp
ATGGTTATGCGACGTGGTCCAATTGGTGGAAGCTCTGGTACTAATACCCGACCAAGACAAACCAATCGTCCCACTTATGAAGATTTCCAGCCCCGCTTTGAATTGAAGGAAGAGCAGGAAGCTCACATAGCACTTGTTCATCTGCCTGGTTTTGTGAAGGAGCAGGTGAGGATTACTATTGAGCATAACCCTAATGTGATTAGGGTTCATGGACAAAGACCTCTGGGCAACAACAAATCGAGTCGTTTCAACCAAACATTTCCGATTCAAGAAAATTGCGACGTAAGTAAAATTGATGCCAAATTTAACGATGGGATTCTTACCATCACAATACCAAAGCCAGTCATCACACAGACAGCAGCAACCAAACCAGTCCAAGAGCCACCACCGAAAACCGCTGCAACCGCTCCCAAGCCGATGCCACAGAAAGTTCAAGAGGACCCAATTCCTTCAAAGCCGAGTTTGACAACACCGGCCGCTGCTGCGTCAAAGGCAGAAAAGGCTACTTTCACTGCCAGAGATGAAAAAGAAAAGGATGACAAGAATGGTGGTCGTCCATTAGGCACTCCGAAAGGCACTGCAGCCGAGACAAAAGCACAAAAGGATAAGGAGGACGCGGCTCTTCCAATACCAGTTTCATCGACCCAGAATTTCAAACAAAGGGATGAGAAGAGGCTGTTACCTTTGACTAGTCCGGAAACAGGCATCGGCATGCCCAAGTCTCAAGAGCATGGTCATGATGGAGAGAGGGGAAAGGCTTCTGTCGAGAGTGTGTCTCGAAAGCGGGTTGAAAACAGGGATCAGAGAGAAGCAGATGGGAAGCCTGATGCAGAACCGAAGAATGCTGAGAAACTTAACAAAATTACTCTACATAAGGAAACCGGTGAGAGAAGCAAAGAAGCAAGGGGTCTCGGTAAGCCTAAAGAGGACTCCGTGGCCGCAAATGTGATGGGTGGTGCAAAGCAGCATATGAAAAACCTCGGGAACGGGATGAGCGATGAAGAGAAGCAGATGTTGATCAATATGGGCGCGGCAGTTCTAGTTCTTCTGGCACTAGGGGCTTCTGCATCCTACAATATCTGGTCCTCTGCAACAGGCAAAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

369

Amino Acids

40.07

Weight (kDa)

9.49

Isoelectric Point (pI)

39.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013354)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G04890
fragaria_vesca FvH4_7g05240
malus_domestica MD02G1259500.v1.1 MD07G1061000.v1.1 MD07G1061100.v1.1
prunus_persica Prupe.2G065600_v2.0.a1 Prupe.2G065600_v2.0.a1
pyrus_communis pycom02g22090
rosa_chinensis RchiOBHm_Chr1g0331581
rosa_laevigata RLG00000029728
rosa_multiflora Rmu_sc0008675.1_g000002
rosa_roxburghii Rroxscaffold_4G00319040
rosa_rugosa Rorug01G0094900
rosa_samantha Rh1AG118700 Rh1BG090400 Rh1CG113700 Rh1DG122900
rosa_wichuraiana Rw1G009510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 154
Acc36I ACCTGC 1 cut(s) 154
AccB7I CCANNNNNTGG 1 cut(s) 26
AccBSI CCGCTC 1 cut(s) 407
AccII CGCG 2 cut(s) 617, 1031
AccIII TCCGGA 1 cut(s) 694
AciI CCGC 8 cut(s) 94, 396, 405, 477, 617, 784, 939, 1031
AclWI GGATC 1 cut(s) 807
AcoI YGGCCR 2 cut(s) 474, 936
AcsI RAATTY 2 cut(s) 302, 649
AfaI GTAC 1 cut(s) 40
AfiI CCNNNNNNNGG 2 cut(s) 26, 701
AgeI ACCGGT 1 cut(s) 881
AgsI TTSAA 8 cut(s) 101, 106, 250, 269, 434, 453, 655, 791
AhlI ACTAGT 1 cut(s) 689
AjiI CACGTC 1 cut(s) 14
AjnI CCWGG 1 cut(s) 145
AjuI GAANNNNNNNTTGG 2 cut(s) 619, 651
AluBI AGCT 2 cut(s) 33, 122
AluI AGCT 2 cut(s) 33, 122
Alw26I GTCTC 4 cut(s) 581, 726, 780, 914
AlwI GGATC 1 cut(s) 807
Ama87I CYCGRG 1 cut(s) 980
Aor13HI TCCGGA 1 cut(s) 694
AoxI GGCC 2 cut(s) 474, 936
ApeKI GCWGC 6 cut(s) 359, 398, 479, 482, 581, 964
ApoI RAATTY 2 cut(s) 302, 649
ArsI GACNNNNNNTTYG 2 cut(s) 52, 84
AsiGI ACCGGT 1 cut(s) 881
Asp700I GAANNNNTTC 1 cut(s) 757
AspLEI GCGC 1 cut(s) 1031
AspS9I GGNCC 3 cut(s) 17, 439, 1083
AsuHPI GGTGA 2 cut(s) 178, 896
AvaI CYCGRG 1 cut(s) 980
AvaII GGWCC 3 cut(s) 17, 439, 1083
BarI GAAGNNNNNNTAC 2 cut(s) 22, 54
BbvI GCAGC 6 cut(s) 371, 385, 466, 469, 593, 976
BccI CCATC 5 cut(s) 305, 332, 734, 809, 943
BciT130I CCWGG 1 cut(s) 147
BclI TGATCA 1 cut(s) 1017
BcoDI GTCTC 4 cut(s) 581, 726, 780, 914
BcuI ACTAGT 1 cut(s) 689
BfaI CTAG 3 cut(s) 690, 1040, 1055
BfmI CTRYAG 1 cut(s) 579
BfuAI ACCTGC 1 cut(s) 154
Bme1390I CCNGG 1 cut(s) 147
Bme18I GGWCC 3 cut(s) 17, 439, 1083
BmeT110I CYCGRG 1 cut(s) 980
BmgBI CACGTC 1 cut(s) 14
BmgT120I GGNCC 3 cut(s) 17, 439, 1083
BmiI GGNNCC 1 cut(s) 441
BmrFI CCNGG 1 cut(s) 147
BmsI GCATC 5 cut(s) 286, 408, 714, 817, 1076
BplI GAGNNNNNCTC 2 cut(s) 760, 792
BpuEI CTTGAG 1 cut(s) 708
BsaBI GATNNNNATC 1 cut(s) 1016
BsaI GGTCTC 1 cut(s) 914
BsaJI CCNNGG 2 cut(s) 933, 979
BsaWI WCCGGW 2 cut(s) 694, 881
Bsc4I CCNNNNNNNGG 2 cut(s) 26, 701
Bse118I RCCGGY 2 cut(s) 472, 881
Bse1I ACTGG 3 cut(s) 341, 371, 632
Bse8I GATNNNNATC 1 cut(s) 1016
BseAI TCCGGA 1 cut(s) 694
BseBI CCWGG 1 cut(s) 147
BseDI CCNNGG 2 cut(s) 933, 979
BseGI GGATG 4 cut(s) 541, 670, 996, 1067
BseJI GATNNNNATC 1 cut(s) 1016
BseLI CCNNNNNNNGG 2 cut(s) 26, 701
BseMII CTCAG 1 cut(s) 837
BseNI ACTGG 3 cut(s) 341, 371, 632
BseX3I CGGCCG 1 cut(s) 474
BseXI GCAGC 6 cut(s) 371, 385, 466, 469, 593, 976
Bsh1236I CGCG 2 cut(s) 617, 1031
Bsh1285I CGRYCG 1 cut(s) 477
BshFI GGCC 2 cut(s) 476, 938
BshTI ACCGGT 1 cut(s) 881
BsiEI CGRYCG 1 cut(s) 477
BsiHKCI CYCGRG 1 cut(s) 980
BsiSI CCGG 3 cut(s) 473, 695, 882
BslFI GGGAC 1 cut(s) 54
BslI CCNNNNNNNGG 2 cut(s) 26, 701
BsmAI GTCTC 4 cut(s) 581, 726, 780, 914
BsmFI GGGAC 1 cut(s) 54
BsmI GAATGC 1 cut(s) 847
BsnI GGCC 2 cut(s) 476, 938
Bso31I GGTCTC 1 cut(s) 914
BsoBI CYCGRG 1 cut(s) 980
Bsp13I TCCGGA 1 cut(s) 694
Bsp143I GATC 2 cut(s) 799, 1017
BspACI CCGC 8 cut(s) 94, 396, 405, 477, 617, 784, 939, 1031
BspANI GGCC 2 cut(s) 476, 938
BspCNI CTCAG 1 cut(s) 838
BspEI TCCGGA 1 cut(s) 694
BspFNI CGCG 2 cut(s) 617, 1031
BspHI TCATGA 1 cut(s) 736
BspLI GGNNCC 1 cut(s) 441
BspMAI CTGCAG 1 cut(s) 583
BspMI ACCTGC 1 cut(s) 154
BspPI GGATC 1 cut(s) 807
BspQI GCTCTTC 2 cut(s) 105, 627
BspTNI GGTCTC 1 cut(s) 914
BsrBI CCGCTC 1 cut(s) 407
BsrFI RCCGGY 2 cut(s) 472, 881
BsrI ACTGG 3 cut(s) 341, 371, 632
BssAI RCCGGY 2 cut(s) 472, 881
BssECI CCNNGG 2 cut(s) 933, 979
BssMI GATC 2 cut(s) 799, 1017
Bst2UI CCWGG 1 cut(s) 147
Bst6I CTCTTC 4 cut(s) 105, 627, 665, 996
BstC8I GCNNGC 1 cut(s) 713
BstDEI CTNAG 1 cut(s) 846
BstDSI CCRYGG 1 cut(s) 933
BstF5I GGATG 4 cut(s) 541, 670, 996, 1067
BstFNI CGCG 2 cut(s) 617, 1031
BstHHI GCGC 1 cut(s) 1031
BstKTI GATC 2 cut(s) 802, 1020
BstMAI GTCTC 4 cut(s) 581, 726, 780, 914
BstMBI GATC 2 cut(s) 799, 1017
BstMCI CGRYCG 1 cut(s) 477
BstMWI GCNNNNNNNGC 6 cut(s) 128, 404, 482, 491, 500, 1058
BstNI CCWGG 1 cut(s) 147
BstNSI RCATGY 1 cut(s) 715
BstSCI CCNGG 1 cut(s) 145
BstSFI CTRYAG 1 cut(s) 579
BstUI CGCG 2 cut(s) 617, 1031
BstV1I GCAGC 6 cut(s) 371, 385, 466, 469, 593, 976
BstZI CGGCCG 1 cut(s) 474
BsuRI GGCC 2 cut(s) 476, 938
BtgI CCRYGG 1 cut(s) 933
BtgZI GCGATG 1 cut(s) 1011
BtrI CACGTC 1 cut(s) 14
BtsCI GGATG 4 cut(s) 541, 670, 996, 1067
BtsI GCAGTG 2 cut(s) 510, 576
BtsIMutI CAGTG 2 cut(s) 510, 576
BveI ACCTGC 1 cut(s) 154
Cac8I GCNNGC 1 cut(s) 713
CciI TCATGA 1 cut(s) 736
CfoI GCGC 1 cut(s) 1031
Cfr10I RCCGGY 2 cut(s) 472, 881
Cfr13I GGNCC 3 cut(s) 17, 439, 1083
CseI GACGC 2 cut(s) 474, 623
Csp6I GTAC 1 cut(s) 39
CspAI ACCGGT 1 cut(s) 881
CspCI CAANNNNNGTGG 2 cut(s) 529, 564
CviAII CATG 4 cut(s) 209, 712, 731, 737
CviQI GTAC 1 cut(s) 39
DdeI CTNAG 1 cut(s) 846
DpnI GATC 2 cut(s) 801, 1019
DpnII GATC 2 cut(s) 799, 1017
EaeI YGGCCR 2 cut(s) 474, 936
EagI CGGCCG 1 cut(s) 474
Eam1104I CTCTTC 4 cut(s) 105, 627, 665, 996
EarI CTCTTC 4 cut(s) 105, 627, 665, 996
EclXI CGGCCG 1 cut(s) 474
Eco31I GGTCTC 1 cut(s) 914
Eco47I GGWCC 3 cut(s) 17, 439, 1083
Eco52I CGGCCG 1 cut(s) 474
Eco88I CYCGRG 1 cut(s) 980
EcoO109I RGGNCCY 1 cut(s) 439
EcoRII CCWGG 1 cut(s) 145
FaeI CATG 4 cut(s) 212, 715, 734, 740
FaqI GGGAC 1 cut(s) 54
FatI CATG 4 cut(s) 208, 711, 730, 736
FauI CCCGC 2 cut(s) 101, 777
FauNDI CATATG 1 cut(s) 969
FbaI TGATCA 1 cut(s) 1017
FokI GGATG 4 cut(s) 548, 677, 1003, 1054
FspBI CTAG 3 cut(s) 690, 1040, 1055
GlaI GCGC 1 cut(s) 1030
HaeIII GGCC 2 cut(s) 476, 938
HapII CCGG 3 cut(s) 473, 695, 882
HgaI GACGC 2 cut(s) 474, 623
HhaI GCGC 1 cut(s) 1031
Hin1II CATG 4 cut(s) 212, 715, 734, 740
Hin6I GCGC 1 cut(s) 1029
HinP1I GCGC 1 cut(s) 1029
HinfI GANTC 4 cut(s) 241, 265, 316, 929
HpaII CCGG 3 cut(s) 473, 695, 882
HphI GGTGA 2 cut(s) 178, 896
Hpy188I TCNGA 3 cut(s) 264, 570, 804
Hpy188III TCNNGA 8 cut(s) 269, 434, 695, 725, 737, 766, 777, 982
Hpy99I CGWCG 2 cut(s) 15, 284
HpyAV CCTTC 3 cut(s) 100, 151, 459
HpyCH4IV ACGT 2 cut(s) 13, 282
HpyCH4V TGCA 6 cut(s) 401, 581, 830, 959, 1067, 1091
HpyF10VI GCNNNNNNNGC 6 cut(s) 128, 404, 482, 491, 500, 1058
HpyF3I CTNAG 1 cut(s) 846
HpySE526I ACGT 2 cut(s) 13, 282
Hsp92II CATG 4 cut(s) 212, 715, 734, 740
HspAI GCGC 1 cut(s) 1029
Kpn2I TCCGGA 1 cut(s) 694
Ksp22I TGATCA 1 cut(s) 1017
Kzo9I GATC 2 cut(s) 799, 1017
LguI GCTCTTC 2 cut(s) 105, 627
LmnI GCTCC 2 cut(s) 160, 412
Lsp1109I GCAGC 6 cut(s) 371, 385, 466, 469, 593, 976
LweI GCATC 5 cut(s) 286, 408, 714, 817, 1076
MaeI CTAG 3 cut(s) 690, 1040, 1055
MaeII ACGT 2 cut(s) 13, 282
MaeIII GTNAC 1 cut(s) 678
MalI GATC 2 cut(s) 801, 1019
MbiI CCGCTC 1 cut(s) 407
MboI GATC 2 cut(s) 799, 1017
MboII GAAGA 7 cut(s) 92, 122, 614, 682, 850, 1013, 1037
MfeI CAATTG 1 cut(s) 21
MluCI AATT 8 cut(s) 21, 101, 274, 291, 302, 444, 649, 861
MlyI GAGTC 2 cut(s) 250, 923
MnlI CCTC 9 cut(s) 162, 231, 430, 604, 666, 741, 919, 989, 1096
MroI TCCGGA 1 cut(s) 694
MroXI GAANNNNTTC 1 cut(s) 757
MseI TTAA 2 cut(s) 306, 855
MslI CAYNNNNRTG 2 cut(s) 710, 735
MspA1I CMGCKG 2 cut(s) 398, 479
MspI CCGG 3 cut(s) 473, 695, 882
MspR9I CCNGG 1 cut(s) 147
MunI CAATTG 1 cut(s) 21
Mva1269I GAATGC 1 cut(s) 847
MvaI CCWGG 1 cut(s) 147
MvnI CGCG 2 cut(s) 617, 1031
MwoI GCNNNNNNNGC 6 cut(s) 128, 404, 482, 491, 500, 1058
NdeI CATATG 1 cut(s) 969
NdeII GATC 2 cut(s) 799, 1017
NlaIII CATG 4 cut(s) 212, 715, 734, 740
NlaIV GGNNCC 1 cut(s) 441
NmeAIII GCCGAG 2 cut(s) 483, 610
NspI RCATGY 1 cut(s) 715
PaeI GCATGC 1 cut(s) 715
PagI TCATGA 1 cut(s) 736
PaqCI CACCTGC 1 cut(s) 154
PciSI GCTCTTC 2 cut(s) 105, 627
PcsI WCGNNNNNNNCGW 1 cut(s) 993
PctI GAATGC 1 cut(s) 847
PdmI GAANNNNTTC 1 cut(s) 757
PfeI GAWTC 2 cut(s) 265, 316
PflFI GACNNNGTC 1 cut(s) 15
PflMI CCANNNNNTGG 1 cut(s) 26
PinAI ACCGGT 1 cut(s) 881
PleI GAGTC 2 cut(s) 249, 923
PpsI GAGTC 2 cut(s) 249, 923
PpuMI RGGWCCY 1 cut(s) 439
Psp5II RGGWCCY 1 cut(s) 439
Psp6I CCWGG 1 cut(s) 145
PspGI CCWGG 1 cut(s) 145
PspN4I GGNNCC 1 cut(s) 441
PspPI GGNCC 3 cut(s) 17, 439, 1083
PspPPI RGGWCCY 1 cut(s) 439
PstI CTGCAG 1 cut(s) 583
PsyI GACNNNGTC 1 cut(s) 15
RsaI GTAC 1 cut(s) 40
RsaNI GTAC 1 cut(s) 39
RseI CAYNNNNRTG 2 cut(s) 710, 735
SapI GCTCTTC 2 cut(s) 105, 627
SaqAI TTAA 2 cut(s) 306, 855
Sau3AI GATC 2 cut(s) 799, 1017
Sau96I GGNCC 3 cut(s) 17, 439, 1083
SchI GAGTC 2 cut(s) 250, 923
ScrFI CCNGG 1 cut(s) 147
SetI ASST 8 cut(s) 16, 35, 124, 168, 223, 285, 685, 981
SfaNI GCATC 5 cut(s) 286, 408, 714, 817, 1076
SfcI CTRYAG 1 cut(s) 579
SinI GGWCC 3 cut(s) 17, 439, 1083
SmiMI CAYNNNNRTG 2 cut(s) 710, 735
SmlI CTYRAG 1 cut(s) 723
SmoI CTYRAG 1 cut(s) 723
SpeI ACTAGT 1 cut(s) 689
SphI GCATGC 1 cut(s) 715
Sse9I AATT 8 cut(s) 21, 101, 274, 291, 302, 444, 649, 861
SsiI CCGC 8 cut(s) 94, 396, 405, 477, 617, 784, 939, 1031
SspMI CTAG 3 cut(s) 690, 1040, 1055
StyD4I CCNGG 1 cut(s) 145
TaiI ACGT 2 cut(s) 16, 285
TaqI TCGA 4 cut(s) 239, 641, 765, 778
TasI AATT 8 cut(s) 21, 101, 274, 291, 302, 444, 649, 861
TauI GCSGC 4 cut(s) 479, 620, 941, 1034
TfiI GAWTC 2 cut(s) 265, 316
Tru1I TTAA 2 cut(s) 306, 855
Tru9I TTAA 2 cut(s) 306, 855
TscAI CASTG 2 cut(s) 517, 583
TseI GCWGC 6 cut(s) 359, 398, 479, 482, 581, 964
TspDTI ATGAA 7 cut(s) 93, 128, 197, 537, 627, 986, 1014
TspGWI ACGGA 1 cut(s) 922
TspRI CASTG 2 cut(s) 517, 583
Tth111I GACNNNGTC 1 cut(s) 15
Van91I CCANNNNNTGG 1 cut(s) 26
VpaK11BI GGWCC 3 cut(s) 17, 439, 1083
XapI RAATTY 2 cut(s) 302, 649
XceI RCATGY 1 cut(s) 715
XmnI GAANNNNTTC 1 cut(s) 757
XspI CTAG 3 cut(s) 690, 1040, 1055
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.