Rh1CG113700

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0013354
Physical Location & Seq
Reverse (-)
22913732 .. 22915020
1289 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG113700.1

Sequence Viewer

Length: 1191 bp
ATGGCTATGAGGCAACGCGTTGGTGCTACTAATACACGTCCAAGACAATCCATTCGTCCCACTTATGAGGATTTCCAGCCCCAGTTTGAATTGAAGGAAGAACCTGGAGCTCACATCATATATGTTCAACTTCCTGGTTTTTTGAAAGAGCAAGTAAGGATTACATATGTGCACGCCCCTGGTGTGATCAGGGTCCATGGACAACGACCCCTTGGCAACAACAGATGGAGCCGTTTCAACGAAACCTTTCCTCTTCCACAAAATTGCCATGAAAGTAAAATTCATGGAAAGTTTAACAATGGCGTTCTTACCATTACATTGCCAAAGGAAGTCCCAAAAGTAGATCCTAAAGAAGCAGCGGCTGCAGCCAAGCCAATCCAAGAGACAAAGCCAAAAGCGGCTGCTGCTACTGAGGCAAAGCCACAGAAAGTCTTGGAGGACGACAAGGTTCCTTCGAAAGAGAAGGACCAGATACGTGCTCCTCCATCTCAAAAACCTGCTGCTACTGCTGCCCGAGACGAAAAACAAAAGGATAAGATAAGTATTCCATCTAAAGACCTCCAAGTAGCTGCTCCTCCAACTCAAAAACCTGCTGTCGAGCCCAATTTACAAAAGGGTCAGGATGCCATTCACCAAACAGCTATTGCTACTGCTGCTAAAGACCCCCAAGTAGCTGTTCCAGAGCCAAAGGCACAAAGGGATGAGAAGAGGGTGATCGCGTTGAGTAGTGCTGCAAGTGAGCCCAAACCTGCTGCTCAAAAGGGTAGGGATGAAATTCCACCAAAAACTATGGGAGATGAAAAGGCGAAGGCGGACTACAAAAAGGAGGTTCAGGATAGATACATAAGCGAGGAAAATGAGAAGCCGGGAGAACCAAAGATTGCTGAGAATATTGTCGGCACACCTCTGAGTAAGATGACTGGTGAGAGAAGCACAGAAGAAGCTGTAATCGAAAGGTTTGCCCCAAATACTTTAGATAAGATAAAGAAGAAGAGCAAACGTTTTGGCAGTGGCGTTGGGGAAGAGGTTTGCAATTCTGCAAAGCATGGGATAAAAAATCTAGACAAGTGGGTAAATAATGAAGAAAAGCAGCTGTTAATCAATGTGGGGGCAGCAGTTCTTGTTCTTGTGGCATTTGGGGCTTACACGACCTACAACTTATGGAGCTCCTCCGGAAAACCCAAGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

396

Amino Acids

43.66

Weight (kDa)

9.44

Isoelectric Point (pI)

40.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 31 - 118 4.5e-11 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013354)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G04890
fragaria_vesca FvH4_7g05240
malus_domestica MD02G1259500.v1.1 MD07G1061000.v1.1 MD07G1061100.v1.1
prunus_persica Prupe.2G065600_v2.0.a1 Prupe.2G065600_v2.0.a1
pyrus_communis pycom02g22090
rosa_chinensis RchiOBHm_Chr1g0331581
rosa_laevigata RLG00000029728
rosa_multiflora Rmu_sc0008675.1_g000002
rosa_roxburghii Rroxscaffold_4G00319040
rosa_rugosa Rorug01G0094900
rosa_samantha Rh1AG118700 Rh1BG090400 Rh1CG113700 Rh1DG122900
rosa_wichuraiana Rw1G009510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 505, 598, 757
AccII CGCG 2 cut(s) 18, 719
AccIII TCCGGA 1 cut(s) 1172
AciI CCGC 3 cut(s) 359, 398, 812
AclI AACGTT 1 cut(s) 1000
AclWI GGATC 1 cut(s) 338
AcsI RAATTY 2 cut(s) 279, 774
AflIII ACRYGT 2 cut(s) 16, 35
AgsI TTSAA 5 cut(s) 89, 94, 128, 145, 238
AjiI CACGTC 1 cut(s) 38
AjnI CCWGG 3 cut(s) 103, 133, 178
AjuI GAANNNNNNNTTGG 2 cut(s) 660, 692
AluBI AGCT 7 cut(s) 110, 569, 641, 674, 944, 1093, 1167
AluI AGCT 7 cut(s) 110, 569, 641, 674, 944, 1093, 1167
Alw21I GWGCWC 4 cut(s) 112, 174, 481, 1169
Alw26I GTCTC 2 cut(s) 377, 510
Alw44I GTGCAC 1 cut(s) 170
AlwI GGATC 1 cut(s) 338
AlwNI CAGNNNCTG 1 cut(s) 362
Ama87I CYCGRG 1 cut(s) 513
Aor13HI TCCGGA 1 cut(s) 1172
ApaLI GTGCAC 1 cut(s) 170
ApoI RAATTY 2 cut(s) 279, 774
ArsI GACNNNNNNTTYG 2 cut(s) 36, 68
Asp700I GAANNNNTTC 1 cut(s) 246
AspS9I GGNCC 2 cut(s) 193, 466
AsuC2I CCSGG 1 cut(s) 867
AsuHPI GGTGA 3 cut(s) 623, 724, 935
AsuII TTCGAA 1 cut(s) 455
AvaI CYCGRG 1 cut(s) 513
AvaII GGWCC 2 cut(s) 193, 466
BaeGI GKGCMC 1 cut(s) 174
BanII GRGCYC 4 cut(s) 112, 603, 744, 1169
BarI GAAGNNNNNNTAC 2 cut(s) 800, 832
Bbv12I GWGCWC 4 cut(s) 112, 174, 481, 1169
BccI CCATC 3 cut(s) 219, 493, 556
BceAI ACGGC 1 cut(s) 216
BcgI CGANNNNNNTGC 2 cut(s) 941, 975
BciT130I CCWGG 3 cut(s) 105, 135, 180
BclI TGATCA 1 cut(s) 186
BcnI CCSGG 1 cut(s) 867
BcoDI GTCTC 2 cut(s) 377, 510
BfaI CTAG 1 cut(s) 1061
BfmI CTRYAG 1 cut(s) 363
BfuAI ACCTGC 3 cut(s) 505, 598, 757
Bme1390I CCNGG 4 cut(s) 105, 135, 180, 867
Bme18I GGWCC 2 cut(s) 193, 466
BmeT110I CYCGRG 1 cut(s) 513
BmgBI CACGTC 1 cut(s) 38
BmgT120I GGNCC 2 cut(s) 193, 466
BmiI GGNNCC 3 cut(s) 194, 230, 450
BmrFI CCNGG 4 cut(s) 105, 135, 180, 867
BmrI ACTGGG 1 cut(s) 76
BmsI GCATC 1 cut(s) 613
BmuI ACTGGG 1 cut(s) 76
BpmI CTGGAG 1 cut(s) 126
Bpu14I TTCGAA 1 cut(s) 455
BpuMI CCSGG 1 cut(s) 867
BsaAI YACGTR 1 cut(s) 476
BsaJI CCNNGG 3 cut(s) 178, 196, 211
BsaWI WCCGGW 1 cut(s) 1172
Bse1I ACTGG 2 cut(s) 82, 925
Bse3DI GCAATG 1 cut(s) 317
BseAI TCCGGA 1 cut(s) 1172
BseBI CCWGG 3 cut(s) 105, 135, 180
BseDI CCNNGG 3 cut(s) 178, 196, 211
BseGI GGATG 3 cut(s) 628, 706, 775
BseMI GCAATG 1 cut(s) 317
BseMII CTCAG 3 cut(s) 402, 876, 899
BseNI ACTGG 2 cut(s) 82, 925
BseRI GAGGAG 3 cut(s) 471, 564, 1159
BseSI GKGCMC 1 cut(s) 174
Bsh1236I CGCG 2 cut(s) 18, 719
BsiHKAI GWGCWC 4 cut(s) 112, 174, 481, 1169
BsiHKCI CYCGRG 1 cut(s) 513
BsiSI CCGG 2 cut(s) 866, 1173
BslFI GGGAC 2 cut(s) 42, 317
BsmAI GTCTC 2 cut(s) 377, 510
BsmBI CGTCTC 1 cut(s) 510
BsmFI GGGAC 2 cut(s) 42, 317
BsoBI CYCGRG 1 cut(s) 513
Bsp119I TTCGAA 1 cut(s) 455
Bsp1286I GDGCHC 6 cut(s) 112, 174, 481, 603, 744, 1169
Bsp13I TCCGGA 1 cut(s) 1172
Bsp143I GATC 3 cut(s) 186, 343, 714
Bsp19I CCATGG 1 cut(s) 196
BspACI CCGC 3 cut(s) 359, 398, 812
BspCNI CTCAG 3 cut(s) 403, 877, 900
BspEI TCCGGA 1 cut(s) 1172
BspFNI CGCG 2 cut(s) 18, 719
BspLI GGNNCC 3 cut(s) 194, 230, 450
BspMAI CTGCAG 1 cut(s) 367
BspMI ACCTGC 3 cut(s) 505, 598, 757
BspPI GGATC 1 cut(s) 338
BspQI GCTCTTC 1 cut(s) 986
BspT104I TTCGAA 1 cut(s) 455
BsrDI GCAATG 1 cut(s) 317
BsrI ACTGG 2 cut(s) 82, 925
BssECI CCNNGG 3 cut(s) 178, 196, 211
BssMI GATC 3 cut(s) 186, 343, 714
BssT1I CCWWGG 2 cut(s) 196, 211
Bst2UI CCWGG 3 cut(s) 105, 135, 180
Bst6I CTCTTC 4 cut(s) 258, 701, 986, 1017
BstAPI GCANNNNNTGC 1 cut(s) 362
BstBAI YACGTR 1 cut(s) 476
BstBI TTCGAA 1 cut(s) 455
BstC8I GCNNGC 1 cut(s) 174
BstDEI CTNAG 3 cut(s) 411, 885, 908
BstDSI CCRYGG 1 cut(s) 196
BstF5I GGATG 3 cut(s) 628, 706, 775
BstFNI CGCG 2 cut(s) 18, 719
BstKTI GATC 3 cut(s) 189, 346, 717
BstMAI GTCTC 2 cut(s) 377, 510
BstMBI GATC 3 cut(s) 186, 343, 714
BstMWI GCNNNNNNNGC 8 cut(s) 362, 365, 404, 413, 506, 509, 653, 1139
BstNI CCWGG 3 cut(s) 105, 135, 180
BstSCI CCNGG 4 cut(s) 103, 133, 178, 865
BstSFI CTRYAG 1 cut(s) 363
BstSLI GKGCMC 1 cut(s) 174
BstUI CGCG 2 cut(s) 18, 719
BstX2I RGATCY 1 cut(s) 343
BstYI RGATCY 1 cut(s) 343
BtgI CCRYGG 1 cut(s) 196
BtrI CACGTC 1 cut(s) 38
BtsCI GGATG 3 cut(s) 628, 706, 775
BtsI GCAGTG 1 cut(s) 1015
BtsIMutI CAGTG 1 cut(s) 1015
BveI ACCTGC 3 cut(s) 505, 598, 757
Cac8I GCNNGC 1 cut(s) 174
CaiI CAGNNNCTG 1 cut(s) 362
Cfr13I GGNCC 2 cut(s) 193, 466
CviAII CATG 4 cut(s) 197, 269, 284, 1046
DdeI CTNAG 3 cut(s) 411, 885, 908
DpnI GATC 3 cut(s) 188, 345, 716
DpnII GATC 3 cut(s) 186, 343, 714
Eam1104I CTCTTC 4 cut(s) 258, 701, 986, 1017
EarI CTCTTC 4 cut(s) 258, 701, 986, 1017
EciI GGCGGA 1 cut(s) 827
Ecl136II GAGCTC 2 cut(s) 110, 1167
Eco130I CCWWGG 2 cut(s) 196, 211
Eco24I GRGCYC 4 cut(s) 112, 603, 744, 1169
Eco47I GGWCC 2 cut(s) 193, 466
Eco53kI GAGCTC 2 cut(s) 110, 1167
Eco88I CYCGRG 1 cut(s) 513
EcoICRI GAGCTC 2 cut(s) 110, 1167
EcoRII CCWGG 3 cut(s) 103, 133, 178
EcoT14I CCWWGG 2 cut(s) 196, 211
EcoT38I GRGCYC 4 cut(s) 112, 603, 744, 1169
ErhI CCWWGG 2 cut(s) 196, 211
Esp3I CGTCTC 1 cut(s) 510
FaeI CATG 4 cut(s) 200, 272, 287, 1049
FaqI GGGAC 2 cut(s) 42, 317
FatI CATG 4 cut(s) 196, 268, 283, 1045
FauNDI CATATG 1 cut(s) 166
FbaI TGATCA 1 cut(s) 186
FokI GGATG 3 cut(s) 635, 713, 782
FriOI GRGCYC 4 cut(s) 112, 603, 744, 1169
FspBI CTAG 1 cut(s) 1061
GsuI CTGGAG 1 cut(s) 126
HapII CCGG 2 cut(s) 866, 1173
Hin1II CATG 4 cut(s) 200, 272, 287, 1049
HpaII CCGG 2 cut(s) 866, 1173
HphI GGTGA 3 cut(s) 623, 724, 935
Hpy166II GTNNAC 1 cut(s) 172
Hpy188I TCNGA 1 cut(s) 909
Hpy188III TCNNGA 5 cut(s) 620, 680, 833, 1061, 1173
Hpy8I GTNNAC 1 cut(s) 172
HpyAV CCTTC 4 cut(s) 88, 457, 462, 802
HpyCH4IV ACGT 3 cut(s) 37, 475, 1000
HpyCH4V TGCA 5 cut(s) 172, 365, 734, 1032, 1040
HpyF10VI GCNNNNNNNGC 8 cut(s) 362, 365, 404, 413, 506, 509, 653, 1139
HpyF3I CTNAG 3 cut(s) 411, 885, 908
HpySE526I ACGT 3 cut(s) 37, 475, 1000
Hsp92II CATG 4 cut(s) 200, 272, 287, 1049
Kpn2I TCCGGA 1 cut(s) 1172
Ksp22I TGATCA 1 cut(s) 186
Kzo9I GATC 3 cut(s) 186, 343, 714
LguI GCTCTTC 1 cut(s) 986
LmnI GCTCC 6 cut(s) 107, 228, 484, 577, 1164, 1172
LweI GCATC 1 cut(s) 613
MaeI CTAG 1 cut(s) 1061
MaeII ACGT 3 cut(s) 37, 475, 1000
MalI GATC 3 cut(s) 188, 345, 716
MboI GATC 3 cut(s) 186, 343, 714
MboII GAAGA 8 cut(s) 110, 245, 718, 950, 1000, 1003, 1034, 1094
MflI RGATCY 1 cut(s) 343
MhlI GDGCHC 6 cut(s) 112, 174, 481, 603, 744, 1169
MluCI AATT 6 cut(s) 89, 262, 279, 604, 774, 1033
MluI ACGCGT 1 cut(s) 16
MmeI TCCRAC 1 cut(s) 602
MroI TCCGGA 1 cut(s) 1172
MroXI GAANNNNTTC 1 cut(s) 246
MseI TTAA 2 cut(s) 294, 1097
MspA1I CMGCKG 2 cut(s) 359, 1093
MspI CCGG 2 cut(s) 866, 1173
MspR9I CCNGG 4 cut(s) 105, 135, 180, 867
MvaI CCWGG 3 cut(s) 105, 135, 180
MvnI CGCG 2 cut(s) 18, 719
MwoI GCNNNNNNNGC 8 cut(s) 362, 365, 404, 413, 506, 509, 653, 1139
NciI CCSGG 1 cut(s) 867
NcoI CCATGG 1 cut(s) 196
NdeI CATATG 1 cut(s) 166
NdeII GATC 3 cut(s) 186, 343, 714
NlaIII CATG 4 cut(s) 200, 272, 287, 1049
NlaIV GGNNCC 3 cut(s) 194, 230, 450
NspV TTCGAA 1 cut(s) 455
PciSI GCTCTTC 1 cut(s) 986
PdmI GAANNNNTTC 1 cut(s) 246
Ppu21I YACGTR 1 cut(s) 476
Psp124BI GAGCTC 2 cut(s) 112, 1169
Psp1406I AACGTT 1 cut(s) 1000
Psp6I CCWGG 3 cut(s) 103, 133, 178
PspGI CCWGG 3 cut(s) 103, 133, 178
PspN4I GGNNCC 3 cut(s) 194, 230, 450
PspPI GGNCC 2 cut(s) 193, 466
PstI CTGCAG 1 cut(s) 367
PstNI CAGNNNCTG 1 cut(s) 362
PsuI RGATCY 1 cut(s) 343
PvuII CAGCTG 1 cut(s) 1093
SacI GAGCTC 2 cut(s) 112, 1169
SapI GCTCTTC 1 cut(s) 986
SaqAI TTAA 2 cut(s) 294, 1097
Sau3AI GATC 3 cut(s) 186, 343, 714
Sau96I GGNCC 2 cut(s) 193, 466
ScrFI CCNGG 4 cut(s) 105, 135, 180, 867
SduI GDGCHC 6 cut(s) 112, 174, 481, 603, 744, 1169
SfaNI GCATC 1 cut(s) 613
SfcI CTRYAG 1 cut(s) 363
SfuI TTCGAA 1 cut(s) 455
SinI GGWCC 2 cut(s) 193, 466
Sse9I AATT 6 cut(s) 89, 262, 279, 604, 774, 1033
SsiI CCGC 3 cut(s) 359, 398, 812
SspI AATATT 1 cut(s) 892
SspMI CTAG 1 cut(s) 1061
SstI GAGCTC 2 cut(s) 112, 1169
StyD4I CCNGG 4 cut(s) 103, 133, 178, 865
StyI CCWWGG 2 cut(s) 196, 211
TaiI ACGT 3 cut(s) 40, 478, 1003
TaqI TCGA 3 cut(s) 455, 597, 951
TasI AATT 6 cut(s) 89, 262, 279, 604, 774, 1033
TauI GCSGC 2 cut(s) 362, 401
Tru1I TTAA 2 cut(s) 294, 1097
Tru9I TTAA 2 cut(s) 294, 1097
TscAI CASTG 1 cut(s) 1015
TspDTI ATGAA 5 cut(s) 272, 285, 786, 813, 1095
TspRI CASTG 1 cut(s) 1015
VneI GTGCAC 1 cut(s) 170
VpaK11BI GGWCC 2 cut(s) 193, 466
XapI RAATTY 2 cut(s) 279, 774
XbaI TCTAGA 1 cut(s) 1060
XmnI GAANNNNTTC 1 cut(s) 246
XspI CTAG 1 cut(s) 1061
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.