Rh1DG122900

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0013354
Physical Location & Seq
Reverse (-)
22348210 .. 22349750
1541 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG122900.1

Sequence Viewer

Length: 1188 bp
ATGGCTATGAGGCAACGCGTTGGTGCTACTAATACACGTCCAAGACAATCCATTCATCCCACTTATGAGGATTTCCGGCCCCAGTTTGAATTGAAGGAAGAACCTGAAGCTCACATCATATATGTTCAACTTCCTGGTTTTTTGAAAGAGCAAGTAAGGATTACATTTGTGCACGCCCCTGGTGTGATCAGGGTCCATGGACAACGACCCCTTGGCAACAACAGATGGAGCCGTTTCAACGAAACCTTTCCTCTTCCACAAAATTGCCACGAAAGTAAAATCCAGGGAAAGTTTAACAATGGCGTTCTTACCATTACATTGCCAAAGGAAGTCCCAAAAGTAGATCCTAAAGAAGCAGCGGCTGCAGCCAAGCCAATCCAAGAGACAAAGCCAAAAGCCGCTGCTGCTACTGAGGCAAAGCCACAGAAAGTCTTGGAGGACGACAAGGTTCCTTCGAAAGAGAAGGACCAGATACGTGCTCCTCCATCTCAAAAACCTGCTGCTACTGCTGCCCGAGACGAAAAACAAAAGGATAAGATAAGTATTCCATCTAAAGACCCCCAAGTAGCTGCTCCTCCAACTCAAAAACCTGCTGTCGAGCCCAATTTACAAAAGGGTCAGGATGCCATTCACCAAACAGCTATTGCTACTGCTGCTAAAGACCCCCAAGTAGCTGTTTCAGAGCCAAAGGCACAAAGGGATGAGAAGAGGGTGATCGCGTTGAGTAGTGCTGCAAGTGAGCCCAAACCTGCTCAAAAAGGTAGGGATGAAATTCCACCAAAAACTATGGGAGATGAAAAGGAGAAGGCGGACTACAAAAAGGAGGTTCAGGATAGATACATAAGCGAGGAAAATGAGAAGCCGGGAGAACCAAAGATTGCTGAGAATGTTGTCGGCACACCTCTGAGTAAGATGACTGGTGAGAGAAGCACAGAAGAAGCTGTAATCGAAAGGTTTGCCCCAAATACTTTAGATAAGATAAAGATGAAGAGCAAACGTTTTGGCAGTGGTGTTGGGGAAGAGGTTTGCAATTCTGCAAAGCAAGGGATAAAAAATCTAGACAAGTGGGTAAATAATGAAGAAAAGCAGCTGTTAATCAATGTGGGGGCAGCAGTTCTTGTTCTTGTGGCATTTGGGGCTTACACGACCTACAACTTATGGAGCTCCTCCGGAAAACCCAAGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

395

Amino Acids

43.66

Weight (kDa)

9.31

Isoelectric Point (pI)

41.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 31 - 118 3.9e-11 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013354)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G04890
fragaria_vesca FvH4_7g05240
malus_domestica MD02G1259500.v1.1 MD07G1061000.v1.1 MD07G1061100.v1.1
prunus_persica Prupe.2G065600_v2.0.a1 Prupe.2G065600_v2.0.a1
pyrus_communis pycom02g22090
rosa_chinensis RchiOBHm_Chr1g0331581
rosa_laevigata RLG00000029728
rosa_multiflora Rmu_sc0008675.1_g000002
rosa_roxburghii Rroxscaffold_4G00319040
rosa_rugosa Rorug01G0094900
rosa_samantha Rh1AG118700 Rh1BG090400 Rh1CG113700 Rh1DG122900
rosa_wichuraiana Rw1G009510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 505, 598, 757
AccII CGCG 2 cut(s) 18, 719
AccIII TCCGGA 1 cut(s) 1169
AciI CCGC 3 cut(s) 359, 399, 809
AclI AACGTT 1 cut(s) 997
AclWI GGATC 1 cut(s) 338
AcsI RAATTY 1 cut(s) 771
AcuI CTGAAG 1 cut(s) 126
AflIII ACRYGT 2 cut(s) 16, 35
AgsI TTSAA 5 cut(s) 89, 94, 128, 145, 238
AjiI CACGTC 1 cut(s) 38
AjnI CCWGG 3 cut(s) 133, 178, 282
AluBI AGCT 7 cut(s) 110, 569, 641, 674, 941, 1090, 1164
AluI AGCT 7 cut(s) 110, 569, 641, 674, 941, 1090, 1164
Alw21I GWGCWC 3 cut(s) 174, 481, 1166
Alw26I GTCTC 2 cut(s) 377, 510
Alw44I GTGCAC 1 cut(s) 170
AlwI GGATC 1 cut(s) 338
AlwNI CAGNNNCTG 1 cut(s) 362
Ama87I CYCGRG 1 cut(s) 513
Aor13HI TCCGGA 1 cut(s) 1169
AoxI GGCC 1 cut(s) 77
ApaLI GTGCAC 1 cut(s) 170
ApoI RAATTY 1 cut(s) 771
Asp700I GAANNNNTTC 1 cut(s) 246
AspS9I GGNCC 3 cut(s) 78, 193, 466
AsuC2I CCSGG 1 cut(s) 864
AsuHPI GGTGA 3 cut(s) 623, 724, 932
AsuII TTCGAA 1 cut(s) 455
AvaI CYCGRG 1 cut(s) 513
AvaII GGWCC 2 cut(s) 193, 466
BaeGI GKGCMC 1 cut(s) 174
BanII GRGCYC 3 cut(s) 603, 744, 1166
BarI GAAGNNNNNNTAC 2 cut(s) 797, 829
Bbv12I GWGCWC 3 cut(s) 174, 481, 1166
BccI CCATC 3 cut(s) 219, 493, 556
BceAI ACGGC 1 cut(s) 216
BcgI CGANNNNNNTGC 2 cut(s) 938, 972
BciT130I CCWGG 3 cut(s) 135, 180, 284
BclI TGATCA 1 cut(s) 186
BcnI CCSGG 1 cut(s) 864
BcoDI GTCTC 2 cut(s) 377, 510
BfaI CTAG 1 cut(s) 1058
BfmI CTRYAG 1 cut(s) 363
BfuAI ACCTGC 3 cut(s) 505, 598, 757
Bme1390I CCNGG 4 cut(s) 135, 180, 284, 864
Bme18I GGWCC 2 cut(s) 193, 466
BmeT110I CYCGRG 1 cut(s) 513
BmgBI CACGTC 1 cut(s) 38
BmgT120I GGNCC 3 cut(s) 78, 193, 466
BmiI GGNNCC 4 cut(s) 80, 194, 230, 450
BmrFI CCNGG 4 cut(s) 135, 180, 284, 864
BmrI ACTGGG 1 cut(s) 76
BmsI GCATC 1 cut(s) 613
BmuI ACTGGG 1 cut(s) 76
Bpu14I TTCGAA 1 cut(s) 455
BpuMI CCSGG 1 cut(s) 864
BsaAI YACGTR 1 cut(s) 476
BsaJI CCNNGG 4 cut(s) 178, 196, 211, 283
BsaWI WCCGGW 1 cut(s) 1169
Bse1I ACTGG 2 cut(s) 82, 922
Bse3DI GCAATG 1 cut(s) 317
BseAI TCCGGA 1 cut(s) 1169
BseBI CCWGG 3 cut(s) 135, 180, 284
BseDI CCNNGG 4 cut(s) 178, 196, 211, 283
BseGI GGATG 4 cut(s) 55, 628, 706, 772
BseMI GCAATG 1 cut(s) 317
BseMII CTCAG 3 cut(s) 402, 873, 896
BseNI ACTGG 2 cut(s) 82, 922
BseRI GAGGAG 3 cut(s) 471, 564, 1156
BseSI GKGCMC 1 cut(s) 174
Bsh1236I CGCG 2 cut(s) 18, 719
BshFI GGCC 1 cut(s) 79
BsiHKAI GWGCWC 3 cut(s) 174, 481, 1166
BsiHKCI CYCGRG 1 cut(s) 513
BsiSI CCGG 3 cut(s) 76, 863, 1170
BslFI GGGAC 1 cut(s) 317
BsmAI GTCTC 2 cut(s) 377, 510
BsmBI CGTCTC 1 cut(s) 510
BsmFI GGGAC 1 cut(s) 317
BsnI GGCC 1 cut(s) 79
BsoBI CYCGRG 1 cut(s) 513
Bsp119I TTCGAA 1 cut(s) 455
Bsp1286I GDGCHC 5 cut(s) 174, 481, 603, 744, 1166
Bsp13I TCCGGA 1 cut(s) 1169
Bsp143I GATC 3 cut(s) 186, 343, 714
Bsp19I CCATGG 1 cut(s) 196
BspACI CCGC 3 cut(s) 359, 399, 809
BspANI GGCC 1 cut(s) 79
BspCNI CTCAG 3 cut(s) 403, 874, 897
BspEI TCCGGA 1 cut(s) 1169
BspFNI CGCG 2 cut(s) 18, 719
BspLI GGNNCC 4 cut(s) 80, 194, 230, 450
BspMAI CTGCAG 1 cut(s) 367
BspMI ACCTGC 3 cut(s) 505, 598, 757
BspPI GGATC 1 cut(s) 338
BspQI GCTCTTC 1 cut(s) 983
BspT104I TTCGAA 1 cut(s) 455
BsrDI GCAATG 1 cut(s) 317
BsrI ACTGG 2 cut(s) 82, 922
BssECI CCNNGG 4 cut(s) 178, 196, 211, 283
BssMI GATC 3 cut(s) 186, 343, 714
BssT1I CCWWGG 2 cut(s) 196, 211
Bst2UI CCWGG 3 cut(s) 135, 180, 284
Bst6I CTCTTC 4 cut(s) 258, 701, 983, 1014
BstAPI GCANNNNNTGC 1 cut(s) 362
BstBAI YACGTR 1 cut(s) 476
BstBI TTCGAA 1 cut(s) 455
BstC8I GCNNGC 1 cut(s) 174
BstDEI CTNAG 3 cut(s) 411, 882, 905
BstDSI CCRYGG 1 cut(s) 196
BstF5I GGATG 4 cut(s) 55, 628, 706, 772
BstFNI CGCG 2 cut(s) 18, 719
BstKTI GATC 3 cut(s) 189, 346, 717
BstMAI GTCTC 2 cut(s) 377, 510
BstMBI GATC 3 cut(s) 186, 343, 714
BstMWI GCNNNNNNNGC 8 cut(s) 362, 365, 404, 413, 506, 509, 653, 1136
BstNI CCWGG 3 cut(s) 135, 180, 284
BstSCI CCNGG 4 cut(s) 133, 178, 282, 862
BstSFI CTRYAG 1 cut(s) 363
BstSLI GKGCMC 1 cut(s) 174
BstUI CGCG 2 cut(s) 18, 719
BstX2I RGATCY 1 cut(s) 343
BstYI RGATCY 1 cut(s) 343
BsuRI GGCC 1 cut(s) 79
BtgI CCRYGG 1 cut(s) 196
BtrI CACGTC 1 cut(s) 38
BtsCI GGATG 4 cut(s) 55, 628, 706, 772
BtsI GCAGTG 1 cut(s) 1012
BtsIMutI CAGTG 1 cut(s) 1012
BveI ACCTGC 3 cut(s) 505, 598, 757
Cac8I GCNNGC 1 cut(s) 174
CaiI CAGNNNCTG 1 cut(s) 362
Cfr13I GGNCC 3 cut(s) 78, 193, 466
CviAII CATG 1 cut(s) 197
DdeI CTNAG 3 cut(s) 411, 882, 905
DpnI GATC 3 cut(s) 188, 345, 716
DpnII GATC 3 cut(s) 186, 343, 714
Eam1104I CTCTTC 4 cut(s) 258, 701, 983, 1014
EarI CTCTTC 4 cut(s) 258, 701, 983, 1014
EciI GGCGGA 1 cut(s) 824
Ecl136II GAGCTC 1 cut(s) 1164
Eco130I CCWWGG 2 cut(s) 196, 211
Eco24I GRGCYC 3 cut(s) 603, 744, 1166
Eco47I GGWCC 2 cut(s) 193, 466
Eco53kI GAGCTC 1 cut(s) 1164
Eco57I CTGAAG 1 cut(s) 126
Eco88I CYCGRG 1 cut(s) 513
EcoICRI GAGCTC 1 cut(s) 1164
EcoRII CCWGG 3 cut(s) 133, 178, 282
EcoT14I CCWWGG 2 cut(s) 196, 211
EcoT38I GRGCYC 3 cut(s) 603, 744, 1166
ErhI CCWWGG 2 cut(s) 196, 211
Esp3I CGTCTC 1 cut(s) 510
FaeI CATG 1 cut(s) 200
FaiI YATR 9 cut(s) 8, 66, 119, 121, 123, 198, 788, 842, 1159
FaqI GGGAC 1 cut(s) 317
FatI CATG 1 cut(s) 196
FbaI TGATCA 1 cut(s) 186
FokI GGATG 4 cut(s) 42, 635, 713, 779
FriOI GRGCYC 3 cut(s) 603, 744, 1166
FspBI CTAG 1 cut(s) 1058
HaeIII GGCC 1 cut(s) 79
HapII CCGG 3 cut(s) 76, 863, 1170
Hin1II CATG 1 cut(s) 200
HpaII CCGG 3 cut(s) 76, 863, 1170
HphI GGTGA 3 cut(s) 623, 724, 932
Hpy166II GTNNAC 1 cut(s) 172
Hpy188I TCNGA 2 cut(s) 682, 906
Hpy188III TCNNGA 4 cut(s) 620, 830, 1058, 1170
Hpy8I GTNNAC 1 cut(s) 172
HpyAV CCTTC 4 cut(s) 88, 457, 462, 799
HpyCH4IV ACGT 3 cut(s) 37, 475, 997
HpyCH4V TGCA 5 cut(s) 172, 365, 734, 1029, 1037
HpyF10VI GCNNNNNNNGC 8 cut(s) 362, 365, 404, 413, 506, 509, 653, 1136
HpyF3I CTNAG 3 cut(s) 411, 882, 905
HpySE526I ACGT 3 cut(s) 37, 475, 997
Hsp92II CATG 1 cut(s) 200
Kpn2I TCCGGA 1 cut(s) 1169
Ksp22I TGATCA 1 cut(s) 186
Kzo9I GATC 3 cut(s) 186, 343, 714
LguI GCTCTTC 1 cut(s) 983
LmnI GCTCC 5 cut(s) 228, 484, 577, 1161, 1169
LweI GCATC 1 cut(s) 613
MaeI CTAG 1 cut(s) 1058
MaeII ACGT 3 cut(s) 37, 475, 997
MalI GATC 3 cut(s) 188, 345, 716
MboI GATC 3 cut(s) 186, 343, 714
MboII GAAGA 7 cut(s) 110, 245, 718, 947, 1000, 1031, 1091
MflI RGATCY 1 cut(s) 343
MhlI GDGCHC 5 cut(s) 174, 481, 603, 744, 1166
MluCI AATT 5 cut(s) 89, 262, 604, 771, 1030
MluI ACGCGT 1 cut(s) 16
MmeI TCCRAC 1 cut(s) 602
MroI TCCGGA 1 cut(s) 1169
MroXI GAANNNNTTC 1 cut(s) 246
MseI TTAA 2 cut(s) 294, 1094
MspA1I CMGCKG 3 cut(s) 359, 401, 1090
MspI CCGG 3 cut(s) 76, 863, 1170
MspR9I CCNGG 4 cut(s) 135, 180, 284, 864
MvaI CCWGG 3 cut(s) 135, 180, 284
MvnI CGCG 2 cut(s) 18, 719
MwoI GCNNNNNNNGC 8 cut(s) 362, 365, 404, 413, 506, 509, 653, 1136
NciI CCSGG 1 cut(s) 864
NcoI CCATGG 1 cut(s) 196
NdeII GATC 3 cut(s) 186, 343, 714
NlaIII CATG 1 cut(s) 200
NlaIV GGNNCC 4 cut(s) 80, 194, 230, 450
NspV TTCGAA 1 cut(s) 455
PciSI GCTCTTC 1 cut(s) 983
PdmI GAANNNNTTC 1 cut(s) 246
Ppu21I YACGTR 1 cut(s) 476
Psp124BI GAGCTC 1 cut(s) 1166
Psp1406I AACGTT 1 cut(s) 997
Psp6I CCWGG 3 cut(s) 133, 178, 282
PspGI CCWGG 3 cut(s) 133, 178, 282
PspN4I GGNNCC 4 cut(s) 80, 194, 230, 450
PspPI GGNCC 3 cut(s) 78, 193, 466
PstI CTGCAG 1 cut(s) 367
PstNI CAGNNNCTG 1 cut(s) 362
PsuI RGATCY 1 cut(s) 343
PvuII CAGCTG 1 cut(s) 1090
SacI GAGCTC 1 cut(s) 1166
SapI GCTCTTC 1 cut(s) 983
SaqAI TTAA 2 cut(s) 294, 1094
Sau3AI GATC 3 cut(s) 186, 343, 714
Sau96I GGNCC 3 cut(s) 78, 193, 466
ScrFI CCNGG 4 cut(s) 135, 180, 284, 864
SduI GDGCHC 5 cut(s) 174, 481, 603, 744, 1166
SfaNI GCATC 1 cut(s) 613
SfcI CTRYAG 1 cut(s) 363
SfuI TTCGAA 1 cut(s) 455
SinI GGWCC 2 cut(s) 193, 466
Sse9I AATT 5 cut(s) 89, 262, 604, 771, 1030
SsiI CCGC 3 cut(s) 359, 399, 809
SspMI CTAG 1 cut(s) 1058
SstI GAGCTC 1 cut(s) 1166
StyD4I CCNGG 4 cut(s) 133, 178, 282, 862
StyI CCWWGG 2 cut(s) 196, 211
TaiI ACGT 3 cut(s) 40, 478, 1000
TaqI TCGA 3 cut(s) 455, 597, 948
TasI AATT 5 cut(s) 89, 262, 604, 771, 1030
TauI GCSGC 2 cut(s) 362, 401
Tru1I TTAA 2 cut(s) 294, 1094
Tru9I TTAA 2 cut(s) 294, 1094
TscAI CASTG 1 cut(s) 1012
TspDTI ATGAA 5 cut(s) 44, 783, 810, 1001, 1092
TspRI CASTG 1 cut(s) 1012
VneI GTGCAC 1 cut(s) 170
VpaK11BI GGWCC 2 cut(s) 193, 466
XapI RAATTY 1 cut(s) 771
XbaI TCTAGA 1 cut(s) 1057
XmnI GAANNNNTTC 1 cut(s) 246
XspI CTAG 1 cut(s) 1058
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.