FvH4_7g05240

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0013354
Physical Location & Seq
Forward (+)
5576772 .. 5578342
1571 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g05240.t1

Sequence Viewer

Length: 1149 bp
ATGGCTACGAGGCAACGCGTTGGTGCTGCTAATACTTCACGTCCAAGACAATCCATTCGTCCCACTTATGAGGATTTCCAGCCCCACTTTGAATTGAAGGAAGAACCTGAAGCTCACATCATATATGTTCAACTTCCTGGTTTTCTGAAAGAGCAAGTGAGGATTACATTTGTGCATAACCCTGGTGTGATCAGGGTCCATGGACAACGACCCCTTGGCAACAACAGATGGAGCCGTTTTAACCAAACCTTTCCTCTTCCACAAAATTGTCACGAAAGTAAAATTCATGGAAAATTTAGCAGTGGAGTTCTCACCATTACATTGCCGAAGGAAGTCCCAAAAGCAGATCCTAATAAAGAAGCAGCAGCAACCAAGCCAATCCAAGATGCAAAGCCAAAAGAGAAGGACCAGATACATGCTCCGCCATCTCAAAAACCGGCCGTCCAGCCCAAGGTACAAAAGGGACAGGATGCCACTCATCAAACTGCTATTGCTACTGCTGCTAAAGATCCCCAAGTAGCTGCTTCAGAGCCGGAGGCACAAAAGGGACCCCAACTAAAACAAGGGATAGCCGAGACACAAGACCACATGATTAAGGAAGCAGGAGTTGAACCAAATGTTGTTTCAGCAATGACTCAGAATGAAAAACAAAGTGATGATAAGAGGGTGATCGCGTTGAGTAACACTGCAAGTCTTGCCAAACCTCAAAAGGCCGGGGATGAAATTCCACCCAAAACTATGGCAGACCAAAAGGCCAAGGCGGACTATCAAAATGAGGTTCAGGATAGATACATAACTGATGCAAATGAGAAGCCGGGAGAACCAAAGAATACTGAGAAGGCTGTCGACACGCCTCTGAGTAAGGAAAAGGAGATGACTGGTGAGAGAAGCAGCGTAGCATCTGTGATCGATAGGATTGCCCCAAATACTTTAGATCAGGTAAAGAAGACGAGTAAACGTTTGGGTAGTGTCGAGCAAGAGAAGCCTGAAAATGATTGCATTGCTGCAAAGCAACGAATGAAAAATCAGGACAAGTGGATAAATAATGAAGAAAAGCAGCTGTTAATCAATGTGGGGGCTGCAGTTCTTGTTCTTGTGGCATTCGGGGCTTACACGACTTACAGCTTATGGTGCTCCGGAAAACCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

383

Amino Acids

42.29

Weight (kDa)

9.04

Isoelectric Point (pI)

37.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 32 - 118 5.4e-11 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013354)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G04890
fragaria_vesca FvH4_7g05240
malus_domestica MD02G1259500.v1.1 MD07G1061000.v1.1 MD07G1061100.v1.1
prunus_persica Prupe.2G065600_v2.0.a1 Prupe.2G065600_v2.0.a1
pyrus_communis pycom02g22090
rosa_chinensis RchiOBHm_Chr1g0331581
rosa_laevigata RLG00000029728
rosa_multiflora Rmu_sc0008675.1_g000002
rosa_roxburghii Rroxscaffold_4G00319040
rosa_rugosa Rorug01G0094900
rosa_samantha Rh1AG118700 Rh1BG090400 Rh1CG113700 Rh1DG122900
rosa_wichuraiana Rw1G009510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 846
AccII CGCG 2 cut(s) 18, 674
AccIII TCCGGA 1 cut(s) 1136
AciI CCGC 2 cut(s) 422, 761
AclI AACGTT 1 cut(s) 958
AclWI GGATC 2 cut(s) 341, 503
AcoI YGGCCR 1 cut(s) 438
AcsI RAATTY 3 cut(s) 282, 293, 723
AcuI CTGAAG 2 cut(s) 129, 510
AfaI GTAC 1 cut(s) 456
AfiI CCNNNNNNNGG 1 cut(s) 451
AflIII ACRYGT 1 cut(s) 16
AgsI TTSAA 4 cut(s) 92, 97, 131, 611
AjiI CACGTC 1 cut(s) 41
AjnI CCWGG 2 cut(s) 136, 181
AjuI GAANNNNNNNTTGG 2 cut(s) 607, 639
AluBI AGCT 4 cut(s) 113, 521, 1060, 1125
AluI AGCT 4 cut(s) 113, 521, 1060, 1125
Alw21I GWGCWC 1 cut(s) 1136
Alw26I GTCTC 1 cut(s) 569
AlwI GGATC 2 cut(s) 341, 503
Aor13HI TCCGGA 1 cut(s) 1136
AoxI GGCC 3 cut(s) 438, 711, 753
ApeKI GCWGC 9 cut(s) 26, 362, 365, 500, 521, 891, 1004, 1057, 1079
ApoI RAATTY 3 cut(s) 282, 293, 723
ArsI GACNNNNNNTTYG 2 cut(s) 39, 71
AspS9I GGNCC 3 cut(s) 196, 406, 548
AsuC2I CCSGG 2 cut(s) 715, 816
AsuHPI GGTGA 3 cut(s) 304, 679, 893
AvaII GGWCC 3 cut(s) 196, 406, 548
BbsI GAAGAC 1 cut(s) 953
Bbv12I GWGCWC 1 cut(s) 1136
BbvI GCAGC 9 cut(s) 13, 374, 377, 487, 508, 903, 991, 1066, 1069
BccI CCATC 2 cut(s) 222, 433
BceAI ACGGC 2 cut(s) 219, 425
BcgI CGANNNNNNTGC 2 cut(s) 899, 933
BciT130I CCWGG 2 cut(s) 138, 183
BclI TGATCA 1 cut(s) 189
BcnI CCSGG 2 cut(s) 715, 816
BcoDI GTCTC 1 cut(s) 569
BfaI CTAG 1 cut(s) 1147
BfmI CTRYAG 1 cut(s) 1080
BisI GCNGC 9 cut(s) 27, 363, 366, 501, 522, 892, 1005, 1058, 1080
BlsI GCNGC 9 cut(s) 28, 364, 367, 502, 523, 893, 1006, 1059, 1081
Bme1390I CCNGG 4 cut(s) 138, 183, 715, 816
Bme18I GGWCC 3 cut(s) 196, 406, 548
BmgBI CACGTC 1 cut(s) 41
BmgT120I GGNCC 3 cut(s) 196, 406, 548
BmiI GGNNCC 4 cut(s) 197, 233, 549, 550
BmrFI CCNGG 4 cut(s) 138, 183, 715, 816
BmsI GCATC 4 cut(s) 376, 460, 790, 908
BpiI GAAGAC 1 cut(s) 953
BpuMI CCSGG 2 cut(s) 715, 816
Bsa29I ATCGAT 1 cut(s) 909
BsaJI CCNNGG 6 cut(s) 181, 199, 214, 450, 714, 756
BsaWI WCCGGW 1 cut(s) 1136
Bsc4I CCNNNNNNNGG 1 cut(s) 451
Bse118I RCCGGY 1 cut(s) 436
Bse1I ACTGG 1 cut(s) 883
Bse3DI GCAATG 3 cut(s) 320, 636, 999
BseAI TCCGGA 1 cut(s) 1136
BseBI CCWGG 2 cut(s) 138, 183
BseCI ATCGAT 1 cut(s) 909
BseDI CCNNGG 6 cut(s) 181, 199, 214, 450, 714, 756
BseGI GGATG 2 cut(s) 475, 724
BseLI CCNNNNNNNGG 1 cut(s) 451
BseMI GCAATG 3 cut(s) 320, 636, 999
BseMII CTCAG 3 cut(s) 650, 825, 848
BseNI ACTGG 1 cut(s) 883
BseX3I CGGCCG 1 cut(s) 438
BseXI GCAGC 9 cut(s) 13, 374, 377, 487, 508, 903, 991, 1066, 1069
Bsh1236I CGCG 2 cut(s) 18, 674
Bsh1285I CGRYCG 1 cut(s) 441
BshFI GGCC 3 cut(s) 440, 713, 755
BshVI ATCGAT 1 cut(s) 909
BsiEI CGRYCG 1 cut(s) 441
BsiHKAI GWGCWC 1 cut(s) 1136
BsiSI CCGG 5 cut(s) 437, 533, 714, 815, 1137
BslFI GGGAC 4 cut(s) 45, 320, 477, 561
BslI CCNNNNNNNGG 1 cut(s) 451
BsmAI GTCTC 1 cut(s) 569
BsmFI GGGAC 4 cut(s) 45, 320, 477, 561
BsmI GAATGC 1 cut(s) 1100
BsnI GGCC 3 cut(s) 440, 713, 755
Bsp1286I GDGCHC 1 cut(s) 1136
Bsp13I TCCGGA 1 cut(s) 1136
Bsp143I GATC 6 cut(s) 189, 346, 508, 669, 906, 934
Bsp19I CCATGG 1 cut(s) 199
BspACI CCGC 2 cut(s) 422, 761
BspANI GGCC 3 cut(s) 440, 713, 755
BspCNI CTCAG 3 cut(s) 649, 826, 849
BspDI ATCGAT 1 cut(s) 909
BspEI TCCGGA 1 cut(s) 1136
BspFNI CGCG 2 cut(s) 18, 674
BspLI GGNNCC 4 cut(s) 197, 233, 549, 550
BspMAI CTGCAG 1 cut(s) 1084
BspPI GGATC 2 cut(s) 341, 503
BsrDI GCAATG 3 cut(s) 320, 636, 999
BsrFI RCCGGY 1 cut(s) 436
BsrI ACTGG 1 cut(s) 883
BssAI RCCGGY 1 cut(s) 436
BssECI CCNNGG 6 cut(s) 181, 199, 214, 450, 714, 756
BssMI GATC 6 cut(s) 189, 346, 508, 669, 906, 934
BssT1I CCWWGG 4 cut(s) 199, 214, 450, 756
Bst2UI CCWGG 2 cut(s) 138, 183
Bst6I CTCTTC 1 cut(s) 261
BstAPI GCANNNNNTGC 1 cut(s) 695
BstDEI CTNAG 3 cut(s) 636, 834, 857
BstDSI CCRYGG 1 cut(s) 199
BstF5I GGATG 2 cut(s) 475, 724
BstFNI CGCG 2 cut(s) 18, 674
BstKTI GATC 6 cut(s) 192, 349, 511, 672, 909, 937
BstMAI GTCTC 1 cut(s) 569
BstMBI GATC 6 cut(s) 189, 346, 508, 669, 906, 934
BstMCI CGRYCG 1 cut(s) 441
BstMWI GCNNNNNNNGC 5 cut(s) 500, 695, 982, 1106, 1131
BstNI CCWGG 2 cut(s) 138, 183
BstNSI RCATGY 1 cut(s) 419
BstSCI CCNGG 4 cut(s) 136, 181, 713, 814
BstSFI CTRYAG 1 cut(s) 1080
BstUI CGCG 2 cut(s) 18, 674
BstV1I GCAGC 9 cut(s) 13, 374, 377, 487, 508, 903, 991, 1066, 1069
BstV2I GAAGAC 1 cut(s) 953
BstX2I RGATCY 2 cut(s) 346, 508
BstXI CCANNNNNNTGG 1 cut(s) 739
BstYI RGATCY 2 cut(s) 346, 508
BstZI CGGCCG 1 cut(s) 438
Bsu15I ATCGAT 1 cut(s) 909
BsuRI GGCC 3 cut(s) 440, 713, 755
BsuTUI ATCGAT 1 cut(s) 909
BtgI CCRYGG 1 cut(s) 199
BtrI CACGTC 1 cut(s) 41
BtsCI GGATG 2 cut(s) 475, 724
BtsI GCAGTG 2 cut(s) 307, 684
BtsIMutI CAGTG 2 cut(s) 307, 684
Cfr10I RCCGGY 1 cut(s) 436
Cfr13I GGNCC 3 cut(s) 196, 406, 548
ClaI ATCGAT 1 cut(s) 909
Csp6I GTAC 1 cut(s) 455
CviAII CATG 4 cut(s) 200, 287, 416, 589
CviQI GTAC 1 cut(s) 455
DdeI CTNAG 3 cut(s) 636, 834, 857
DpnI GATC 6 cut(s) 191, 348, 510, 671, 908, 936
DpnII GATC 6 cut(s) 189, 346, 508, 669, 906, 934
EaeI YGGCCR 1 cut(s) 438
EagI CGGCCG 1 cut(s) 438
Eam1104I CTCTTC 1 cut(s) 261
EarI CTCTTC 1 cut(s) 261
EciI GGCGGA 2 cut(s) 411, 776
EclXI CGGCCG 1 cut(s) 438
Eco130I CCWWGG 4 cut(s) 199, 214, 450, 756
Eco47I GGWCC 3 cut(s) 196, 406, 548
Eco52I CGGCCG 1 cut(s) 438
Eco57I CTGAAG 2 cut(s) 129, 510
EcoO109I RGGNCCY 1 cut(s) 548
EcoRII CCWGG 2 cut(s) 136, 181
EcoT14I CCWWGG 4 cut(s) 199, 214, 450, 756
ErhI CCWWGG 4 cut(s) 199, 214, 450, 756
FaeI CATG 4 cut(s) 203, 290, 419, 592
FaqI GGGAC 4 cut(s) 45, 320, 477, 561
FatI CATG 4 cut(s) 199, 286, 415, 588
FbaI TGATCA 1 cut(s) 189
FblI GTMKAC 1 cut(s) 846
Fnu4HI GCNGC 9 cut(s) 27, 363, 366, 501, 522, 892, 1005, 1058, 1080
FokI GGATG 2 cut(s) 482, 731
Fsp4HI GCNGC 9 cut(s) 27, 363, 366, 501, 522, 892, 1005, 1058, 1080
FspBI CTAG 1 cut(s) 1147
GluI GCNGC 9 cut(s) 27, 363, 366, 501, 522, 892, 1005, 1058, 1080
HaeIII GGCC 3 cut(s) 440, 713, 755
HapII CCGG 5 cut(s) 437, 533, 714, 815, 1137
Hin1II CATG 4 cut(s) 203, 290, 419, 592
HincII GTYRAC 1 cut(s) 847
HindII GTYRAC 1 cut(s) 847
HinfI GANTC 1 cut(s) 634
HpaII CCGG 5 cut(s) 437, 533, 714, 815, 1137
HphI GGTGA 3 cut(s) 304, 679, 893
Hpy166II GTNNAC 2 cut(s) 847, 956
Hpy188I TCNGA 4 cut(s) 147, 529, 639, 858
Hpy188III TCNNGA 4 cut(s) 272, 782, 1028, 1137
Hpy8I GTNNAC 2 cut(s) 847, 956
HpyAV CCTTC 4 cut(s) 91, 322, 397, 832
HpyCH4IV ACGT 2 cut(s) 40, 958
HpyCH4V TGCA 7 cut(s) 175, 389, 689, 803, 999, 1007, 1082
HpyF10VI GCNNNNNNNGC 5 cut(s) 500, 695, 982, 1106, 1131
HpyF3I CTNAG 3 cut(s) 636, 834, 857
HpySE526I ACGT 2 cut(s) 40, 958
Hsp92II CATG 4 cut(s) 203, 290, 419, 592
KflI GGGWCCC 1 cut(s) 548
Kpn2I TCCGGA 1 cut(s) 1136
Ksp22I TGATCA 1 cut(s) 189
Kzo9I GATC 6 cut(s) 189, 346, 508, 669, 906, 934
LmnI GCTCC 3 cut(s) 231, 424, 1139
Lsp1109I GCAGC 9 cut(s) 13, 374, 377, 487, 508, 903, 991, 1066, 1069
LweI GCATC 4 cut(s) 376, 460, 790, 908
MaeI CTAG 1 cut(s) 1147
MaeII ACGT 2 cut(s) 40, 958
MaeIII GTNAC 2 cut(s) 269, 680
MalI GATC 6 cut(s) 191, 348, 510, 671, 908, 936
MboI GATC 6 cut(s) 189, 346, 508, 669, 906, 934
MboII GAAGA 4 cut(s) 113, 248, 958, 1061
MflI RGATCY 2 cut(s) 346, 508
MhlI GDGCHC 1 cut(s) 1136
MluCI AATT 5 cut(s) 92, 265, 282, 293, 723
MluI ACGCGT 1 cut(s) 16
MlyI GAGTC 1 cut(s) 628
MnlI CCTC 9 cut(s) 3, 64, 153, 264, 529, 657, 714, 769, 864
MroI TCCGGA 1 cut(s) 1136
MseI TTAA 3 cut(s) 240, 594, 1064
MspA1I CMGCKG 1 cut(s) 1060
MspI CCGG 5 cut(s) 437, 533, 714, 815, 1137
MspR9I CCNGG 4 cut(s) 138, 183, 715, 816
Mva1269I GAATGC 1 cut(s) 1100
MvaI CCWGG 2 cut(s) 138, 183
MvnI CGCG 2 cut(s) 18, 674
MwoI GCNNNNNNNGC 5 cut(s) 500, 695, 982, 1106, 1131
NciI CCSGG 2 cut(s) 715, 816
NcoI CCATGG 1 cut(s) 199
NdeII GATC 6 cut(s) 189, 346, 508, 669, 906, 934
NlaIII CATG 4 cut(s) 203, 290, 419, 592
NlaIV GGNNCC 4 cut(s) 197, 233, 549, 550
NmeAIII GCCGAG 1 cut(s) 598
NmuCI GTSAC 1 cut(s) 269
NspI RCATGY 1 cut(s) 419
PctI GAATGC 1 cut(s) 1100
PkrI GCNGC 9 cut(s) 28, 364, 367, 502, 523, 893, 1006, 1059, 1081
PleI GAGTC 1 cut(s) 628
PpsI GAGTC 1 cut(s) 628
PpuMI RGGWCCY 1 cut(s) 548
Psp1406I AACGTT 1 cut(s) 958
Psp5II RGGWCCY 1 cut(s) 548
Psp6I CCWGG 2 cut(s) 136, 181
PspGI CCWGG 2 cut(s) 136, 181
PspN4I GGNNCC 4 cut(s) 197, 233, 549, 550
PspPI GGNCC 3 cut(s) 196, 406, 548
PspPPI RGGWCCY 1 cut(s) 548
PstI CTGCAG 1 cut(s) 1084
PsuI RGATCY 2 cut(s) 346, 508
PvuII CAGCTG 1 cut(s) 1060
RsaI GTAC 1 cut(s) 456
RsaNI GTAC 1 cut(s) 455
SalI GTCGAC 1 cut(s) 845
SaqAI TTAA 3 cut(s) 240, 594, 1064
SatI GCNGC 9 cut(s) 27, 363, 366, 501, 522, 892, 1005, 1058, 1080
Sau3AI GATC 6 cut(s) 189, 346, 508, 669, 906, 934
Sau96I GGNCC 3 cut(s) 196, 406, 548
SchI GAGTC 1 cut(s) 628
ScrFI CCNGG 4 cut(s) 138, 183, 715, 816
SduI GDGCHC 1 cut(s) 1136
SfaNI GCATC 4 cut(s) 376, 460, 790, 908
SfcI CTRYAG 1 cut(s) 1080
SinI GGWCC 3 cut(s) 196, 406, 548
Sse9I AATT 5 cut(s) 92, 265, 282, 293, 723
SsiI CCGC 2 cut(s) 422, 761
SspMI CTAG 1 cut(s) 1147
StyD4I CCNGG 4 cut(s) 136, 181, 713, 814
StyI CCWWGG 4 cut(s) 199, 214, 450, 756
TaiI ACGT 2 cut(s) 43, 961
TaqI TCGA 3 cut(s) 846, 909, 972
TasI AATT 5 cut(s) 92, 265, 282, 293, 723
Tru1I TTAA 3 cut(s) 240, 594, 1064
Tru9I TTAA 3 cut(s) 240, 594, 1064
TscAI CASTG 2 cut(s) 307, 691
TseFI GTSAC 1 cut(s) 269
TseI GCWGC 9 cut(s) 26, 362, 365, 500, 521, 891, 1004, 1057, 1079
Tsp45I GTSAC 1 cut(s) 269
TspDTI ATGAA 5 cut(s) 275, 657, 735, 1034, 1062
TspRI CASTG 2 cut(s) 307, 691
VpaK11BI GGWCC 3 cut(s) 196, 406, 548
XapI RAATTY 3 cut(s) 282, 293, 723
XceI RCATGY 1 cut(s) 419
XmiI GTMKAC 1 cut(s) 846
XspI CTAG 1 cut(s) 1147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.