MD07G1061000.v1.1

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0013354
Physical Location & Seq
Forward (+)
5717363 .. 5718874
1512 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1061000.v1.1.491

Sequence Viewer

Length: 1062 bp
ATGTCTATGGGACATCGAGAGGGAGAGATGCCTATGGGACATCGTCCTCCTGGTGGAAGCTCTGATGCTAATACCCGCCCAAGACAATCCCATCGTCCCATTTATGAAGATTTCCAGCCCTACTTTGAATTGAAGGAAGAGCAGGAAGCTCACATTGTACAAGTTCATCTGCCTGGTTTTAAGAAGGAGCAGGCTAGGATTACTTATATGTATAACCATGGTGTGATTAGGGTTCATGGACAAAGACCTCTCGACAACAACACATGGAGCCGTTTCGACCAAACATTTCCGGTTCAACATAATTGCGACGCAACTAAAATTCATGCCAAATTTAGCAATGGGATTCTTACCATTACAATACCAAAGAAATTCATCACATGGACTGCAACAGCCGAACCTGTTGCAACCACGGTCAAGCCGAAGCCACACAAAGTTCAAGAGGACCCAATTCCTTCAAAGCCGAGTTTGACAACACCGGCAGGCCCTCCGACAGACACCTCTTCGGATACAAAGGGTCGGGAAGTTATTTCTCAAACAGCTGCTGATTCTGCTCAAGGTGAACAACAAAGGGGCAAGACAAGTGCTCAGGAAGCTGCTGCTGCTGCCGCGTTGAAGACAGAAAAGGCTACTTTCACTGCCAGAGATGAAAAAGAAATGGATGACAAGAATGGTCGTCCACCATTAGCTCCTCCGAAAGGCACTGCAGCTGAGACAAAAGCACAAGAGGATAAGGAGGTTGAGAAGAGGTTGATACCGTTGACTAGTCCGGAAACAAGCAAAGCCGAGACTAAGTCTGAAGAGGGTGGTCAGGATGGAAAGAGAGGAAAGGCTTCTGTCGAGAGTGTTTATCGAACGGGGGTTGAAAACAAGGATCAGAAAAAAGCAAAAGGGAAGGCTGAAGATGAAGGTTCTATTGCCGCACATGTGATGGCTGCTGCAAATGAGGGTATGAAAAATCTCGGAAGCAGGATGAATGATGAAGAGAAGAAGATGTTGATCAATATGGGTGCAGCGATTCTAGTTCTTATCGCACTAGGTGCTTCTGCATCCTACAGTCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

354

Amino Acids

38.5

Weight (kDa)

8.37

Isoelectric Point (pI)

39.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 44 - 123 1.1e-10 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013354)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G04890
fragaria_vesca FvH4_7g05240
malus_domestica MD02G1259500.v1.1 MD07G1061000.v1.1 MD07G1061100.v1.1
prunus_persica Prupe.2G065600_v2.0.a1 Prupe.2G065600_v2.0.a1
pyrus_communis pycom02g22090
rosa_chinensis RchiOBHm_Chr1g0331581
rosa_laevigata RLG00000029728
rosa_multiflora Rmu_sc0008675.1_g000002
rosa_roxburghii Rroxscaffold_4G00319040
rosa_rugosa Rorug01G0094900
rosa_samantha Rh1AG118700 Rh1BG090400 Rh1CG113700 Rh1DG122900
rosa_wichuraiana Rw1G009510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 608
AccIII TCCGGA 1 cut(s) 766
AciI CCGC 3 cut(s) 76, 606, 918
AclWI GGATC 1 cut(s) 879
AcsI RAATTY 3 cut(s) 318, 329, 368
AcuI CTGAAG 2 cut(s) 816, 918
AdeI CACNNNGTG 1 cut(s) 1037
AfaI GTAC 1 cut(s) 159
AfiI CCNNNNNNNGG 2 cut(s) 53, 695
AflIII ACRYGT 1 cut(s) 922
AgsI TTSAA 7 cut(s) 128, 133, 296, 437, 456, 613, 863
AhlI ACTAGT 1 cut(s) 761
AjnI CCWGG 2 cut(s) 49, 172
AluBI AGCT 6 cut(s) 60, 149, 539, 593, 686, 707
AluI AGCT 6 cut(s) 60, 149, 539, 593, 686, 707
Alw21I GWGCWC 1 cut(s) 586
Alw26I GTCTC 2 cut(s) 704, 779
AlwI GGATC 1 cut(s) 879
AlwNI CAGNNNCTG 1 cut(s) 542
Aor13HI TCCGGA 1 cut(s) 766
AoxI GGCC 1 cut(s) 481
ApeKI GCWGC 9 cut(s) 539, 593, 596, 599, 602, 704, 932, 935, 1010
ApoI RAATTY 3 cut(s) 318, 329, 368
Asp700I GAANNNNTTC 1 cut(s) 829
AspS9I GGNCC 2 cut(s) 442, 482
AsuHPI GGTGA 1 cut(s) 569
AvaII GGWCC 1 cut(s) 442
BbsI GAAGAC 1 cut(s) 620
Bbv12I GWGCWC 1 cut(s) 586
BbvI GCAGC 9 cut(s) 526, 580, 583, 586, 589, 716, 919, 922, 1022
BccI CCATC 3 cut(s) 99, 806, 922
BceAI ACGGC 1 cut(s) 255
BcgI CGANNNNNNTGC 2 cut(s) 383, 417
BciT130I CCWGG 2 cut(s) 51, 174
BciVI GTATCC 1 cut(s) 499
BclI TGATCA 1 cut(s) 996
BcoDI GTCTC 2 cut(s) 704, 779
BcuI ACTAGT 1 cut(s) 761
BfaI CTAG 4 cut(s) 195, 762, 1019, 1034
BfmI CTRYAG 3 cut(s) 702, 1051, 1058
BfuI GTATCC 1 cut(s) 499
Bme1390I CCNGG 2 cut(s) 51, 174
Bme18I GGWCC 1 cut(s) 442
BmgT120I GGNCC 2 cut(s) 442, 482
BmiI GGNNCC 2 cut(s) 269, 444
BmrFI CCNGG 2 cut(s) 51, 174
BmsI GCATC 3 cut(s) 18, 55, 1055
BpiI GAAGAC 1 cut(s) 620
Bpu10I CCTNAGC 1 cut(s) 585
BpuEI CTTGAG 1 cut(s) 537
BsaBI GATNNNNATC 1 cut(s) 995
BsaJI CCNNGG 2 cut(s) 217, 408
BsaWI WCCGGW 2 cut(s) 289, 766
Bsc4I CCNNNNNNNGG 2 cut(s) 53, 695
Bse118I RCCGGY 1 cut(s) 475
Bse3DI GCAATG 1 cut(s) 343
Bse8I GATNNNNATC 1 cut(s) 995
BseAI TCCGGA 1 cut(s) 766
BseBI CCWGG 2 cut(s) 51, 174
BseDI CCNNGG 2 cut(s) 217, 408
BseGI GGATG 4 cut(s) 664, 817, 975, 1046
BseJI GATNNNNATC 1 cut(s) 995
BseLI CCNNNNNNNGG 2 cut(s) 53, 695
BseMI GCAATG 1 cut(s) 343
BseMII CTCAG 2 cut(s) 599, 699
BseRI GAGGAG 1 cut(s) 678
BseXI GCAGC 9 cut(s) 526, 580, 583, 586, 589, 716, 919, 922, 1022
BsgI GTGCAG 1 cut(s) 1029
Bsh1236I CGCG 1 cut(s) 608
BshFI GGCC 1 cut(s) 483
BsiHKAI GWGCWC 1 cut(s) 586
BsiSI CCGG 3 cut(s) 290, 476, 767
BslFI GGGAC 3 cut(s) 24, 51, 81
BslI CCNNNNNNNGG 2 cut(s) 53, 695
BsmAI GTCTC 2 cut(s) 704, 779
BsmFI GGGAC 3 cut(s) 24, 51, 81
BsnI GGCC 1 cut(s) 483
Bsp1286I GDGCHC 1 cut(s) 586
Bsp13I TCCGGA 1 cut(s) 766
Bsp1407I TGTACA 1 cut(s) 157
Bsp143I GATC 2 cut(s) 871, 996
Bsp19I CCATGG 1 cut(s) 217
BspACI CCGC 3 cut(s) 76, 606, 918
BspANI GGCC 1 cut(s) 483
BspCNI CTCAG 2 cut(s) 598, 700
BspEI TCCGGA 1 cut(s) 766
BspFNI CGCG 1 cut(s) 608
BspLI GGNNCC 2 cut(s) 269, 444
BspMAI CTGCAG 1 cut(s) 706
BspPI GGATC 1 cut(s) 879
BspQI GCTCTTC 1 cut(s) 132
BsrDI GCAATG 1 cut(s) 343
BsrFI RCCGGY 1 cut(s) 475
BsrGI TGTACA 1 cut(s) 157
BssAI RCCGGY 1 cut(s) 475
BssECI CCNNGG 2 cut(s) 217, 408
BssMI GATC 2 cut(s) 871, 996
BssT1I CCWWGG 1 cut(s) 217
Bst2UI CCWGG 2 cut(s) 51, 174
Bst4CI ACNGT 3 cut(s) 412, 756, 1055
Bst6I CTCTTC 5 cut(s) 132, 505, 737, 792, 975
BstAPI GCANNNNNTGC 1 cut(s) 1037
BstAUI TGTACA 1 cut(s) 157
BstC8I GCNNGC 2 cut(s) 192, 481
BstDEI CTNAG 3 cut(s) 585, 708, 789
BstDSI CCRYGG 2 cut(s) 217, 408
BstENI CCTNNNNNAGG 1 cut(s) 693
BstF5I GGATG 4 cut(s) 664, 817, 975, 1046
BstFNI CGCG 1 cut(s) 608
BstKTI GATC 2 cut(s) 874, 999
BstMAI GTCTC 2 cut(s) 704, 779
BstMBI GATC 2 cut(s) 871, 996
BstMWI GCNNNNNNNGC 6 cut(s) 548, 590, 599, 602, 605, 1037
BstNI CCWGG 2 cut(s) 51, 174
BstNSI RCATGY 1 cut(s) 926
BstSCI CCNGG 2 cut(s) 49, 172
BstSFI CTRYAG 3 cut(s) 702, 1051, 1058
BstUI CGCG 1 cut(s) 608
BstV1I GCAGC 9 cut(s) 526, 580, 583, 586, 589, 716, 919, 922, 1022
BstV2I GAAGAC 1 cut(s) 620
BsuI GTATCC 1 cut(s) 499
BsuRI GGCC 1 cut(s) 483
BtgI CCRYGG 2 cut(s) 217, 408
BtsCI GGATG 4 cut(s) 664, 817, 975, 1046
BtsI GCAGTG 2 cut(s) 633, 699
BtsIMutI CAGTG 2 cut(s) 633, 699
Cac8I GCNNGC 2 cut(s) 192, 481
CaiI CAGNNNCTG 1 cut(s) 542
Cfr10I RCCGGY 1 cut(s) 475
Cfr13I GGNCC 2 cut(s) 442, 482
CseI GACGC 1 cut(s) 317
Csp6I GTAC 1 cut(s) 158
CviAII CATG 6 cut(s) 218, 236, 264, 323, 378, 923
CviQI GTAC 1 cut(s) 158
DdeI CTNAG 3 cut(s) 585, 708, 789
DpnI GATC 2 cut(s) 873, 998
DpnII GATC 2 cut(s) 871, 996
DraIII CACNNNGTG 1 cut(s) 1037
Eam1104I CTCTTC 5 cut(s) 132, 505, 737, 792, 975
EarI CTCTTC 5 cut(s) 132, 505, 737, 792, 975
Eco130I CCWWGG 1 cut(s) 217
Eco47I GGWCC 1 cut(s) 442
Eco57I CTGAAG 2 cut(s) 816, 918
EcoNI CCTNNNNNAGG 1 cut(s) 693
EcoO109I RGGNCCY 2 cut(s) 442, 482
EcoRII CCWGG 2 cut(s) 49, 172
EcoT14I CCWWGG 1 cut(s) 217
ErhI CCWWGG 1 cut(s) 217
FaeI CATG 6 cut(s) 221, 239, 267, 326, 381, 926
FaqI GGGAC 3 cut(s) 24, 51, 81
FatI CATG 6 cut(s) 217, 235, 263, 322, 377, 922
FauI CCCGC 1 cut(s) 83
FbaI TGATCA 1 cut(s) 996
FokI GGATG 4 cut(s) 671, 824, 982, 1033
FspBI CTAG 4 cut(s) 195, 762, 1019, 1034
HaeIII GGCC 1 cut(s) 483
HapII CCGG 3 cut(s) 290, 476, 767
HgaI GACGC 1 cut(s) 317
Hin1II CATG 6 cut(s) 221, 239, 267, 326, 381, 926
HincII GTYRAC 1 cut(s) 759
HindII GTYRAC 1 cut(s) 759
HinfI GANTC 3 cut(s) 343, 545, 1015
HpaII CCGG 3 cut(s) 290, 476, 767
HphI GGTGA 1 cut(s) 569
Hpy166II GTNNAC 3 cut(s) 560, 677, 759
Hpy188I TCNGA 7 cut(s) 64, 489, 505, 693, 796, 876, 962
Hpy188III TCNNGA 8 cut(s) 17, 251, 437, 518, 587, 767, 809, 838
Hpy8I GTNNAC 3 cut(s) 560, 677, 759
Hpy99I CGWCG 1 cut(s) 311
HpyAV CCTTC 5 cut(s) 127, 178, 462, 886, 899
HpyCH4III ACNGT 3 cut(s) 412, 756, 1055
HpyCH4V TGCA 6 cut(s) 386, 404, 704, 938, 1010, 1046
HpyF10VI GCNNNNNNNGC 6 cut(s) 548, 590, 599, 602, 605, 1037
HpyF3I CTNAG 3 cut(s) 585, 708, 789
Hsp92II CATG 6 cut(s) 221, 239, 267, 326, 381, 926
Kpn2I TCCGGA 1 cut(s) 766
Ksp22I TGATCA 1 cut(s) 996
Kzo9I GATC 2 cut(s) 871, 996
LguI GCTCTTC 1 cut(s) 132
LmnI GCTCC 3 cut(s) 187, 267, 691
Lsp1109I GCAGC 9 cut(s) 526, 580, 583, 586, 589, 716, 919, 922, 1022
LweI GCATC 3 cut(s) 18, 55, 1055
MaeI CTAG 4 cut(s) 195, 762, 1019, 1034
MalI GATC 2 cut(s) 873, 998
MboI GATC 2 cut(s) 871, 996
MhlI GDGCHC 1 cut(s) 586
MluCI AATT 6 cut(s) 128, 301, 318, 329, 368, 447
MmeI TCCRAC 1 cut(s) 512
MroI TCCGGA 1 cut(s) 766
MroXI GAANNNNTTC 1 cut(s) 829
MseI TTAA 1 cut(s) 180
MspA1I CMGCKG 2 cut(s) 539, 707
MspI CCGG 3 cut(s) 290, 476, 767
MspR9I CCNGG 2 cut(s) 51, 174
MvaI CCWGG 2 cut(s) 51, 174
MvnI CGCG 1 cut(s) 608
MwoI GCNNNNNNNGC 6 cut(s) 548, 590, 599, 602, 605, 1037
NcoI CCATGG 1 cut(s) 217
NdeII GATC 2 cut(s) 871, 996
NlaIII CATG 6 cut(s) 221, 239, 267, 326, 381, 926
NlaIV GGNNCC 2 cut(s) 269, 444
NmeAIII GCCGAG 2 cut(s) 486, 808
NspI RCATGY 1 cut(s) 926
PciI ACATGT 1 cut(s) 922
PciSI GCTCTTC 1 cut(s) 132
PcsI WCGNNNNNNNCGW 1 cut(s) 416
PdmI GAANNNNTTC 1 cut(s) 829
PfeI GAWTC 3 cut(s) 343, 545, 1015
PflFI GACNNNGTC 2 cut(s) 42, 790
PpuMI RGGWCCY 1 cut(s) 442
PscI ACATGT 1 cut(s) 922
Psp5II RGGWCCY 1 cut(s) 442
Psp6I CCWGG 2 cut(s) 49, 172
PspGI CCWGG 2 cut(s) 49, 172
PspN4I GGNNCC 2 cut(s) 269, 444
PspPI GGNCC 2 cut(s) 442, 482
PspPPI RGGWCCY 1 cut(s) 442
PstI CTGCAG 1 cut(s) 706
PstNI CAGNNNCTG 1 cut(s) 542
PsyI GACNNNGTC 2 cut(s) 42, 790
PvuII CAGCTG 2 cut(s) 539, 707
RsaI GTAC 1 cut(s) 159
RsaNI GTAC 1 cut(s) 158
SapI GCTCTTC 1 cut(s) 132
SaqAI TTAA 1 cut(s) 180
Sau3AI GATC 2 cut(s) 871, 996
Sau96I GGNCC 2 cut(s) 442, 482
ScrFI CCNGG 2 cut(s) 51, 174
SduI GDGCHC 1 cut(s) 586
SfaNI GCATC 3 cut(s) 18, 55, 1055
SfcI CTRYAG 3 cut(s) 702, 1051, 1058
SinI GGWCC 1 cut(s) 442
SmlI CTYRAG 1 cut(s) 552
SmoI CTYRAG 1 cut(s) 552
SpeI ACTAGT 1 cut(s) 761
Sse9I AATT 6 cut(s) 128, 301, 318, 329, 368, 447
SsiI CCGC 3 cut(s) 76, 606, 918
SspMI CTAG 4 cut(s) 195, 762, 1019, 1034
StyD4I CCNGG 2 cut(s) 49, 172
StyI CCWWGG 1 cut(s) 217
TaaI ACNGT 3 cut(s) 412, 756, 1055
TaqI TCGA 5 cut(s) 16, 252, 276, 837, 850
TasI AATT 6 cut(s) 128, 301, 318, 329, 368, 447
TatI WGTACW 1 cut(s) 157
TauI GCSGC 2 cut(s) 608, 920
TfiI GAWTC 3 cut(s) 343, 545, 1015
Tru1I TTAA 1 cut(s) 180
Tru9I TTAA 1 cut(s) 180
TscAI CASTG 2 cut(s) 640, 706
TseI GCWGC 9 cut(s) 539, 593, 596, 599, 602, 704, 932, 935, 1010
TspRI CASTG 2 cut(s) 640, 706
Tth111I GACNNNGTC 2 cut(s) 42, 790
VpaK11BI GGWCC 1 cut(s) 442
XagI CCTNNNNNAGG 1 cut(s) 693
XapI RAATTY 3 cut(s) 318, 329, 368
XceI RCATGY 1 cut(s) 926
XmnI GAANNNNTTC 1 cut(s) 829
XspI CTAG 4 cut(s) 195, 762, 1019, 1034
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.