Rroxscaffold_4G00319040

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0013354
Physical Location & Seq
Forward (+)
46525400 .. 46526926
1527 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00319040.1

Sequence Viewer

Length: 1185 bp
ATGGCTATGAGGCAACGCGTTGGTGCTACTAATACACGTCCAAGACAATCCAGTCGTCCCACTTATGAGGATTTCCAGCCCCAGTTTGAATTGAAGGAAGAACCTGAAGCTCACATCATATATGTTCAACTTCCTGGTTTTTTGAAAGAGCAAGTAAGGATTACATTTGTGCACGCCCCTGGTGTGATCAGGGTCCATGGACAACGACCCCTTGGCAACAACAGATGGAGCCGTTTCAACGAAACCTTTCCTCTTCCTCAAAATTGCCACGAAAGTAAAATTCATGGAAAGTTCAACAATGGCGTTCTTACCATTACATTGCCAAAGGAAGTCCCAAAAGTAGATCCTAAAGAAGCAGCGGCTGCAGCCAAGCCAATCCAAGAGACAAAGCCAAAAGCCGCTGCTGCTACTGAGGCAAAGCCACAGAAAGTCTTGGAGGACGACAAGGTTCCTTCGAAAGAGAAGGACCAGATACGTGCTCCTCCATCTCAAAAACCTGCTGCTACTGCCGCCCGAGACGAAAAACAAGAGGATAAGATAAGTATTCCATCTAAAGACCCCCAAGTAGCTGCTCCTCCAACTCAAAAACCCGCTGTCGAGCCCAATTTACAAAAGGGTCAGGATGCCATTCACCAAACAGCTATTGCTACTGCTGCTAAAGACGCCCAAGTAGCTGCTCCAGAGCCAAAGGCACAAAGGGATGAGAAGAGGGTGATCGCGTTGAGTAGTGCTGCAAGTGAGCCCAAACCTCAAAAGGGTAGGGATGAAATTCCACCAAAAACTATGGGAGATGAAAAGGCGAAGGCGGACCACAAAAAGGAGGTTCAGGATAGATACATAAGCGAGGAAAATGAGAAGCCGGGAGAACCAAAGATTGCTGAGAATGTTGTCGACACACCTCTGAGTAAGATGACTGGTGAGAGAAGCACAGAAGAAGCTGTAATCGAAAGGTTTGCCCCAAATACTTTAGATAAGATAAAGAAGAAGAGCAAACGTTTTGGCAGTGGTGTTGGGGAAGAGGTTTGCAATTCTGCAAAGCAAGGGATAAAAAATCTAGACAAGTGGGCAAATAATGAAGAAAAGCAGCTGTTAATCAATGTGGGGGCAGCAGTTCTTGTTCTTGTGGCATTTGGGGCTTACACGACCTACAACTTATGGTGCTCCTCCGGAAAACCCAAGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

394

Amino Acids

43.48

Weight (kDa)

9.22

Isoelectric Point (pI)

42.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 31 - 118 3.8e-11 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013354)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G04890
fragaria_vesca FvH4_7g05240
malus_domestica MD02G1259500.v1.1 MD07G1061000.v1.1 MD07G1061100.v1.1
prunus_persica Prupe.2G065600_v2.0.a1 Prupe.2G065600_v2.0.a1
pyrus_communis pycom02g22090
rosa_chinensis RchiOBHm_Chr1g0331581
rosa_laevigata RLG00000029728
rosa_multiflora Rmu_sc0008675.1_g000002
rosa_roxburghii Rroxscaffold_4G00319040
rosa_rugosa Rorug01G0094900
rosa_samantha Rh1AG118700 Rh1BG090400 Rh1CG113700 Rh1DG122900
rosa_wichuraiana Rw1G009510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 51
Acc36I ACCTGC 1 cut(s) 505
AccI GTMKAC 1 cut(s) 891
AccII CGCG 2 cut(s) 18, 719
AccIII TCCGGA 1 cut(s) 1166
AciI CCGC 5 cut(s) 359, 399, 510, 591, 806
AclI AACGTT 1 cut(s) 994
AclWI GGATC 1 cut(s) 338
AcsI RAATTY 2 cut(s) 279, 768
AcuI CTGAAG 1 cut(s) 126
AcyI GRCGYC 1 cut(s) 663
AfiI CCNNNNNNNGG 2 cut(s) 755, 817
AflIII ACRYGT 2 cut(s) 16, 35
AgsI TTSAA 6 cut(s) 89, 94, 128, 145, 238, 295
AjiI CACGTC 1 cut(s) 38
AjnI CCWGG 2 cut(s) 133, 178
AluBI AGCT 6 cut(s) 110, 569, 641, 674, 938, 1087
AluI AGCT 6 cut(s) 110, 569, 641, 674, 938, 1087
Alw21I GWGCWC 3 cut(s) 174, 481, 1163
Alw26I GTCTC 2 cut(s) 377, 510
Alw44I GTGCAC 1 cut(s) 170
AlwI GGATC 1 cut(s) 338
AlwNI CAGNNNCTG 1 cut(s) 362
Ama87I CYCGRG 1 cut(s) 513
Aor13HI TCCGGA 1 cut(s) 1166
ApaLI GTGCAC 1 cut(s) 170
ApoI RAATTY 2 cut(s) 279, 768
Asp700I GAANNNNTTC 1 cut(s) 246
AspS9I GGNCC 3 cut(s) 193, 466, 808
AsuC2I CCSGG 1 cut(s) 861
AsuHPI GGTGA 3 cut(s) 623, 724, 929
AsuII TTCGAA 1 cut(s) 455
AvaI CYCGRG 1 cut(s) 513
AvaII GGWCC 3 cut(s) 193, 466, 808
BaeGI GKGCMC 1 cut(s) 174
BanII GRGCYC 2 cut(s) 603, 744
Bbv12I GWGCWC 3 cut(s) 174, 481, 1163
BccI CCATC 3 cut(s) 219, 493, 556
BceAI ACGGC 1 cut(s) 216
BcgI CGANNNNNNTGC 2 cut(s) 935, 969
BciT130I CCWGG 2 cut(s) 135, 180
BclI TGATCA 1 cut(s) 186
BcnI CCSGG 1 cut(s) 861
BcoDI GTCTC 2 cut(s) 377, 510
BfaI CTAG 1 cut(s) 1055
BfmI CTRYAG 1 cut(s) 363
BfuAI ACCTGC 1 cut(s) 505
Bme1390I CCNGG 3 cut(s) 135, 180, 861
Bme18I GGWCC 3 cut(s) 193, 466, 808
BmeT110I CYCGRG 1 cut(s) 513
BmgBI CACGTC 1 cut(s) 38
BmgT120I GGNCC 3 cut(s) 193, 466, 808
BmiI GGNNCC 3 cut(s) 194, 230, 450
BmrFI CCNGG 3 cut(s) 135, 180, 861
BmrI ACTGGG 1 cut(s) 76
BmsI GCATC 1 cut(s) 613
BmuI ACTGGG 1 cut(s) 76
BpmI CTGGAG 1 cut(s) 663
Bpu14I TTCGAA 1 cut(s) 455
BpuMI CCSGG 1 cut(s) 861
BsaAI YACGTR 1 cut(s) 476
BsaHI GRCGYC 1 cut(s) 663
BsaJI CCNNGG 3 cut(s) 178, 196, 211
BsaWI WCCGGW 1 cut(s) 1166
Bsc4I CCNNNNNNNGG 2 cut(s) 755, 817
Bse1I ACTGG 3 cut(s) 51, 82, 919
Bse3DI GCAATG 1 cut(s) 317
BseAI TCCGGA 1 cut(s) 1166
BseBI CCWGG 2 cut(s) 135, 180
BseDI CCNNGG 3 cut(s) 178, 196, 211
BseGI GGATG 3 cut(s) 628, 706, 769
BseLI CCNNNNNNNGG 2 cut(s) 755, 817
BseMI GCAATG 1 cut(s) 317
BseMII CTCAG 3 cut(s) 402, 870, 893
BseNI ACTGG 3 cut(s) 51, 82, 919
BseRI GAGGAG 3 cut(s) 471, 564, 1153
BseSI GKGCMC 1 cut(s) 174
Bsh1236I CGCG 2 cut(s) 18, 719
BsiHKAI GWGCWC 3 cut(s) 174, 481, 1163
BsiHKCI CYCGRG 1 cut(s) 513
BsiSI CCGG 2 cut(s) 860, 1167
BslFI GGGAC 2 cut(s) 42, 317
BslI CCNNNNNNNGG 2 cut(s) 755, 817
BsmAI GTCTC 2 cut(s) 377, 510
BsmBI CGTCTC 1 cut(s) 510
BsmFI GGGAC 2 cut(s) 42, 317
BsoBI CYCGRG 1 cut(s) 513
Bsp119I TTCGAA 1 cut(s) 455
Bsp1286I GDGCHC 5 cut(s) 174, 481, 603, 744, 1163
Bsp13I TCCGGA 1 cut(s) 1166
Bsp143I GATC 3 cut(s) 186, 343, 714
Bsp19I CCATGG 1 cut(s) 196
BspACI CCGC 5 cut(s) 359, 399, 510, 591, 806
BspCNI CTCAG 3 cut(s) 403, 871, 894
BspEI TCCGGA 1 cut(s) 1166
BspFNI CGCG 2 cut(s) 18, 719
BspLI GGNNCC 3 cut(s) 194, 230, 450
BspMAI CTGCAG 1 cut(s) 367
BspMI ACCTGC 1 cut(s) 505
BspPI GGATC 1 cut(s) 338
BspQI GCTCTTC 1 cut(s) 980
BspT104I TTCGAA 1 cut(s) 455
BsrDI GCAATG 1 cut(s) 317
BsrI ACTGG 3 cut(s) 51, 82, 919
BssECI CCNNGG 3 cut(s) 178, 196, 211
BssMI GATC 3 cut(s) 186, 343, 714
BssNI GRCGYC 1 cut(s) 663
BssT1I CCWWGG 2 cut(s) 196, 211
Bst2UI CCWGG 2 cut(s) 135, 180
Bst6I CTCTTC 4 cut(s) 258, 701, 980, 1011
BstACI GRCGYC 1 cut(s) 663
BstAPI GCANNNNNTGC 1 cut(s) 362
BstBAI YACGTR 1 cut(s) 476
BstBI TTCGAA 1 cut(s) 455
BstC8I GCNNGC 1 cut(s) 174
BstDEI CTNAG 3 cut(s) 411, 879, 902
BstDSI CCRYGG 1 cut(s) 196
BstF5I GGATG 3 cut(s) 628, 706, 769
BstFNI CGCG 2 cut(s) 18, 719
BstKTI GATC 3 cut(s) 189, 346, 717
BstMAI GTCTC 2 cut(s) 377, 510
BstMBI GATC 3 cut(s) 186, 343, 714
BstNI CCWGG 2 cut(s) 135, 180
BstSCI CCNGG 3 cut(s) 133, 178, 859
BstSFI CTRYAG 1 cut(s) 363
BstSLI GKGCMC 1 cut(s) 174
BstUI CGCG 2 cut(s) 18, 719
BstX2I RGATCY 1 cut(s) 343
BstYI RGATCY 1 cut(s) 343
BtgI CCRYGG 1 cut(s) 196
BtrI CACGTC 1 cut(s) 38
BtsCI GGATG 3 cut(s) 628, 706, 769
BtsI GCAGTG 1 cut(s) 1009
BtsIMutI CAGTG 1 cut(s) 1009
BveI ACCTGC 1 cut(s) 505
Cac8I GCNNGC 1 cut(s) 174
CaiI CAGNNNCTG 1 cut(s) 362
Cfr13I GGNCC 3 cut(s) 193, 466, 808
CseI GACGC 1 cut(s) 671
CviAII CATG 2 cut(s) 197, 284
DdeI CTNAG 3 cut(s) 411, 879, 902
DpnI GATC 3 cut(s) 188, 345, 716
DpnII GATC 3 cut(s) 186, 343, 714
DrdI GACNNNNNNGTC 1 cut(s) 51
DseDI GACNNNNNNGTC 1 cut(s) 51
Eam1104I CTCTTC 4 cut(s) 258, 701, 980, 1011
EarI CTCTTC 4 cut(s) 258, 701, 980, 1011
EciI GGCGGA 1 cut(s) 821
Eco130I CCWWGG 2 cut(s) 196, 211
Eco24I GRGCYC 2 cut(s) 603, 744
Eco47I GGWCC 3 cut(s) 193, 466, 808
Eco57I CTGAAG 1 cut(s) 126
Eco88I CYCGRG 1 cut(s) 513
EcoRII CCWGG 2 cut(s) 133, 178
EcoT14I CCWWGG 2 cut(s) 196, 211
EcoT38I GRGCYC 2 cut(s) 603, 744
ErhI CCWWGG 2 cut(s) 196, 211
Esp3I CGTCTC 1 cut(s) 510
FaeI CATG 2 cut(s) 200, 287
FaqI GGGAC 2 cut(s) 42, 317
FatI CATG 2 cut(s) 196, 283
FauI CCCGC 1 cut(s) 598
FbaI TGATCA 1 cut(s) 186
FblI GTMKAC 1 cut(s) 891
FokI GGATG 3 cut(s) 635, 713, 776
FriOI GRGCYC 2 cut(s) 603, 744
FspBI CTAG 1 cut(s) 1055
GsuI CTGGAG 1 cut(s) 663
HapII CCGG 2 cut(s) 860, 1167
HgaI GACGC 1 cut(s) 671
Hin1I GRCGYC 1 cut(s) 663
Hin1II CATG 2 cut(s) 200, 287
HincII GTYRAC 1 cut(s) 892
HindII GTYRAC 1 cut(s) 892
HpaII CCGG 2 cut(s) 860, 1167
HphI GGTGA 3 cut(s) 623, 724, 929
Hpy166II GTNNAC 2 cut(s) 172, 892
Hpy188I TCNGA 1 cut(s) 903
Hpy188III TCNNGA 5 cut(s) 620, 680, 827, 1055, 1167
Hpy8I GTNNAC 2 cut(s) 172, 892
HpyAV CCTTC 4 cut(s) 88, 457, 462, 796
HpyCH4IV ACGT 3 cut(s) 37, 475, 994
HpyCH4V TGCA 5 cut(s) 172, 365, 734, 1026, 1034
HpyF3I CTNAG 3 cut(s) 411, 879, 902
HpySE526I ACGT 3 cut(s) 37, 475, 994
Hsp92I GRCGYC 1 cut(s) 663
Hsp92II CATG 2 cut(s) 200, 287
Kpn2I TCCGGA 1 cut(s) 1166
Ksp22I TGATCA 1 cut(s) 186
Kzo9I GATC 3 cut(s) 186, 343, 714
LguI GCTCTTC 1 cut(s) 980
LmnI GCTCC 5 cut(s) 228, 484, 577, 682, 1166
LweI GCATC 1 cut(s) 613
MaeI CTAG 1 cut(s) 1055
MaeII ACGT 3 cut(s) 37, 475, 994
MalI GATC 3 cut(s) 188, 345, 716
MboI GATC 3 cut(s) 186, 343, 714
MboII GAAGA 8 cut(s) 110, 245, 718, 944, 994, 997, 1028, 1088
MflI RGATCY 1 cut(s) 343
MhlI GDGCHC 5 cut(s) 174, 481, 603, 744, 1163
MluCI AATT 6 cut(s) 89, 262, 279, 604, 768, 1027
MluI ACGCGT 1 cut(s) 16
MmeI TCCRAC 1 cut(s) 602
MroI TCCGGA 1 cut(s) 1166
MroXI GAANNNNTTC 1 cut(s) 246
MseI TTAA 1 cut(s) 1091
MspA1I CMGCKG 4 cut(s) 359, 401, 593, 1087
MspI CCGG 2 cut(s) 860, 1167
MspR9I CCNGG 3 cut(s) 135, 180, 861
MvaI CCWGG 2 cut(s) 135, 180
MvnI CGCG 2 cut(s) 18, 719
NciI CCSGG 1 cut(s) 861
NcoI CCATGG 1 cut(s) 196
NdeII GATC 3 cut(s) 186, 343, 714
NlaIII CATG 2 cut(s) 200, 287
NlaIV GGNNCC 3 cut(s) 194, 230, 450
NspV TTCGAA 1 cut(s) 455
PciSI GCTCTTC 1 cut(s) 980
PdmI GAANNNNTTC 1 cut(s) 246
Ppu21I YACGTR 1 cut(s) 476
Psp1406I AACGTT 1 cut(s) 994
Psp6I CCWGG 2 cut(s) 133, 178
PspGI CCWGG 2 cut(s) 133, 178
PspN4I GGNNCC 3 cut(s) 194, 230, 450
PspPI GGNCC 3 cut(s) 193, 466, 808
PstI CTGCAG 1 cut(s) 367
PstNI CAGNNNCTG 1 cut(s) 362
PsuI RGATCY 1 cut(s) 343
PvuII CAGCTG 1 cut(s) 1087
SalI GTCGAC 1 cut(s) 890
SapI GCTCTTC 1 cut(s) 980
SaqAI TTAA 1 cut(s) 1091
Sau3AI GATC 3 cut(s) 186, 343, 714
Sau96I GGNCC 3 cut(s) 193, 466, 808
ScrFI CCNGG 3 cut(s) 135, 180, 861
SduI GDGCHC 5 cut(s) 174, 481, 603, 744, 1163
SfaNI GCATC 1 cut(s) 613
SfcI CTRYAG 1 cut(s) 363
SfuI TTCGAA 1 cut(s) 455
SinI GGWCC 3 cut(s) 193, 466, 808
Sse9I AATT 6 cut(s) 89, 262, 279, 604, 768, 1027
SsiI CCGC 5 cut(s) 359, 399, 510, 591, 806
SspMI CTAG 1 cut(s) 1055
StyD4I CCNGG 3 cut(s) 133, 178, 859
StyI CCWWGG 2 cut(s) 196, 211
TaiI ACGT 3 cut(s) 40, 478, 997
TaqI TCGA 4 cut(s) 455, 597, 891, 945
TasI AATT 6 cut(s) 89, 262, 279, 604, 768, 1027
TauI GCSGC 3 cut(s) 362, 401, 512
Tru1I TTAA 1 cut(s) 1091
Tru9I TTAA 1 cut(s) 1091
TscAI CASTG 1 cut(s) 1009
TspDTI ATGAA 4 cut(s) 272, 780, 807, 1089
TspRI CASTG 1 cut(s) 1009
VneI GTGCAC 1 cut(s) 170
VpaK11BI GGWCC 3 cut(s) 193, 466, 808
XapI RAATTY 2 cut(s) 279, 768
XbaI TCTAGA 1 cut(s) 1054
XmiI GTMKAC 1 cut(s) 891
XmnI GAANNNNTTC 1 cut(s) 246
XspI CTAG 1 cut(s) 1055
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.