Rmu_sc0008675.1_g000002

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0008675.1
OG0013354
Physical Location & Seq
Forward (+)
24681 .. 25951
1271 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0008675.1_g000002.1.cds

Sequence Viewer

Length: 1173 bp
atgaggcaacgcgttggtgctactaatacacgtcaatccattcgtcccacttatgaggatttccagccccagtttgaattgaaggaagaacctgaagctcacatcatatatgttcaacttcctggttttttgaaagagcaagtaaggattacatttgtgcacgcccctggtgtgatcagggtccatggacaacgaccccttggcaacaacagatggagccgtttcaacgaaacctttcctcttccacaaaattgccacgaaagtaaaattcatggaaagtttcacaatggcgttcttaccattacattgccaaaggaagtcccaaaagtagatcctaaagaagcagcggctgcagccaagccaatccaagagacaaagccaaaagcggctgctgctactgaggcaaagccacagaaagtcttggaggacgacaaggttccttcgaaagagaaggaccagatacgtgctcctccatctcaaaaacctgctgctactgctgcccgagacgaaaaacaaaaggataagataagtattccatctaaagacccccaagtagctgctcctccaactcaaaaacctgctgtcgagcccaatttacaaaagggtcaggatgccattcaccaaacagctattgctactgctgctaaagacccccaagtagctgttccagagccaaaggcacaaagggatgggaagagggtgatcgcgttgagtagtgctgcaagtgagcccaaacctcaaaagggtggggatgaaattccaccaaaaactatgggagatgaaaaggcgaaggcggactacaaaaaggaggttcaggatagatacataagcgaggaaaatgagaagcctggagaaccaaagattgctgagaatgttgtcgacacacctctgggtaagatgactggtaagagaagcacagaagaagctgtaatcgaaaggtttgccccaaacactttagataagatcaagatgaagagcaaacgttttggcagtggtcttggggatgaggtttgcaattctgcaaagcaagggataaaaaatctagacaagtgggtaaataatgaagaaaagcagctgttaatcaatgtgggggcagcagttcttgttcttgtggcatttggggcttacacgacctacaacttatggagctcctccggaaaacccaagaactaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

390

Amino Acids

42.96

Weight (kDa)

9.35

Isoelectric Point (pI)

42.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013354)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G04890
fragaria_vesca FvH4_7g05240
malus_domestica MD02G1259500.v1.1 MD07G1061000.v1.1 MD07G1061100.v1.1
prunus_persica Prupe.2G065600_v2.0.a1 Prupe.2G065600_v2.0.a1
pyrus_communis pycom02g22090
rosa_chinensis RchiOBHm_Chr1g0331581
rosa_laevigata RLG00000029728
rosa_multiflora Rmu_sc0008675.1_g000002
rosa_roxburghii Rroxscaffold_4G00319040
rosa_rugosa Rorug01G0094900
rosa_samantha Rh1AG118700 Rh1BG090400 Rh1CG113700 Rh1DG122900
rosa_wichuraiana Rw1G009510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 493, 586
AccI GTMKAC 1 cut(s) 879
AccII CGCG 2 cut(s) 12, 707
AccIII TCCGGA 1 cut(s) 1154
AciI CCGC 3 cut(s) 347, 386, 794
AclI AACGTT 1 cut(s) 982
AclWI GGATC 1 cut(s) 326
AcsI RAATTY 2 cut(s) 267, 756
AcuI CTGAAG 1 cut(s) 114
AfiI CCNNNNNNNGG 1 cut(s) 743
AflIII ACRYGT 2 cut(s) 10, 29
AgsI TTSAA 5 cut(s) 77, 82, 116, 133, 226
AjiI CACGTC 1 cut(s) 32
AjnI CCWGG 3 cut(s) 121, 166, 847
AjuI GAANNNNNNNTTGG 2 cut(s) 648, 680
AluBI AGCT 7 cut(s) 98, 557, 629, 662, 926, 1075, 1149
AluI AGCT 7 cut(s) 98, 557, 629, 662, 926, 1075, 1149
Alw21I GWGCWC 3 cut(s) 162, 469, 1151
Alw26I GTCTC 2 cut(s) 365, 498
Alw44I GTGCAC 1 cut(s) 158
AlwI GGATC 1 cut(s) 326
AlwNI CAGNNNCTG 1 cut(s) 350
Ama87I CYCGRG 1 cut(s) 501
Aor13HI TCCGGA 1 cut(s) 1154
ApaLI GTGCAC 1 cut(s) 158
ApoI RAATTY 2 cut(s) 267, 756
Asp700I GAANNNNTTC 1 cut(s) 234
AspS9I GGNCC 2 cut(s) 181, 454
AsuHPI GGTGA 2 cut(s) 611, 712
AsuII TTCGAA 1 cut(s) 443
AvaI CYCGRG 1 cut(s) 501
AvaII GGWCC 2 cut(s) 181, 454
BaeGI GKGCMC 1 cut(s) 162
BanII GRGCYC 3 cut(s) 591, 732, 1151
BarI GAAGNNNNNNTAC 2 cut(s) 782, 814
Bbv12I GWGCWC 3 cut(s) 162, 469, 1151
BccI CCATC 4 cut(s) 207, 481, 544, 683
BceAI ACGGC 1 cut(s) 204
BcgI CGANNNNNNTGC 2 cut(s) 923, 957
BciT130I CCWGG 3 cut(s) 123, 168, 849
BclI TGATCA 1 cut(s) 174
BcoDI GTCTC 2 cut(s) 365, 498
BfaI CTAG 1 cut(s) 1043
BfmI CTRYAG 1 cut(s) 351
BfuAI ACCTGC 2 cut(s) 493, 586
Bme1390I CCNGG 3 cut(s) 123, 168, 849
Bme18I GGWCC 2 cut(s) 181, 454
BmeT110I CYCGRG 1 cut(s) 501
BmgBI CACGTC 1 cut(s) 32
BmgT120I GGNCC 2 cut(s) 181, 454
BmiI GGNNCC 3 cut(s) 182, 218, 438
BmrFI CCNGG 3 cut(s) 123, 168, 849
BmrI ACTGGG 1 cut(s) 64
BmsI GCATC 1 cut(s) 601
BmuI ACTGGG 1 cut(s) 64
BpmI CTGGAG 1 cut(s) 870
Bpu14I TTCGAA 1 cut(s) 443
BsaAI YACGTR 1 cut(s) 464
BsaJI CCNNGG 3 cut(s) 166, 184, 199
BsaWI WCCGGW 1 cut(s) 1154
Bsc4I CCNNNNNNNGG 1 cut(s) 743
Bse1I ACTGG 2 cut(s) 70, 907
Bse3DI GCAATG 1 cut(s) 305
BseAI TCCGGA 1 cut(s) 1154
BseBI CCWGG 3 cut(s) 123, 168, 849
BseDI CCNNGG 3 cut(s) 166, 184, 199
BseGI GGATG 4 cut(s) 616, 694, 757, 1009
BseLI CCNNNNNNNGG 1 cut(s) 743
BseMI GCAATG 1 cut(s) 305
BseMII CTCAG 2 cut(s) 390, 858
BseNI ACTGG 2 cut(s) 70, 907
BseRI GAGGAG 3 cut(s) 459, 552, 1141
BseSI GKGCMC 1 cut(s) 162
Bsh1236I CGCG 2 cut(s) 12, 707
BsiHKAI GWGCWC 3 cut(s) 162, 469, 1151
BsiHKCI CYCGRG 1 cut(s) 501
BsiSI CCGG 1 cut(s) 1155
BslFI GGGAC 2 cut(s) 30, 305
BslI CCNNNNNNNGG 1 cut(s) 743
BsmAI GTCTC 2 cut(s) 365, 498
BsmBI CGTCTC 1 cut(s) 498
BsmFI GGGAC 2 cut(s) 30, 305
BsoBI CYCGRG 1 cut(s) 501
Bsp119I TTCGAA 1 cut(s) 443
Bsp1286I GDGCHC 5 cut(s) 162, 469, 591, 732, 1151
Bsp13I TCCGGA 1 cut(s) 1154
Bsp143I GATC 4 cut(s) 174, 331, 702, 963
Bsp19I CCATGG 1 cut(s) 184
BspACI CCGC 3 cut(s) 347, 386, 794
BspCNI CTCAG 2 cut(s) 391, 859
BspEI TCCGGA 1 cut(s) 1154
BspFNI CGCG 2 cut(s) 12, 707
BspLI GGNNCC 3 cut(s) 182, 218, 438
BspMAI CTGCAG 1 cut(s) 355
BspMI ACCTGC 2 cut(s) 493, 586
BspPI GGATC 1 cut(s) 326
BspQI GCTCTTC 1 cut(s) 968
BspT104I TTCGAA 1 cut(s) 443
BsrDI GCAATG 1 cut(s) 305
BsrI ACTGG 2 cut(s) 70, 907
BssECI CCNNGG 3 cut(s) 166, 184, 199
BssMI GATC 4 cut(s) 174, 331, 702, 963
BssT1I CCWWGG 2 cut(s) 184, 199
Bst2UI CCWGG 3 cut(s) 123, 168, 849
Bst6I CTCTTC 3 cut(s) 246, 689, 968
BstAPI GCANNNNNTGC 1 cut(s) 350
BstBAI YACGTR 1 cut(s) 464
BstBI TTCGAA 1 cut(s) 443
BstC8I GCNNGC 1 cut(s) 162
BstDEI CTNAG 2 cut(s) 399, 867
BstDSI CCRYGG 1 cut(s) 184
BstF5I GGATG 4 cut(s) 616, 694, 757, 1009
BstFNI CGCG 2 cut(s) 12, 707
BstKTI GATC 4 cut(s) 177, 334, 705, 966
BstMAI GTCTC 2 cut(s) 365, 498
BstMBI GATC 4 cut(s) 174, 331, 702, 963
BstMWI GCNNNNNNNGC 8 cut(s) 350, 353, 392, 401, 494, 497, 641, 1121
BstNI CCWGG 3 cut(s) 123, 168, 849
BstSCI CCNGG 3 cut(s) 121, 166, 847
BstSFI CTRYAG 1 cut(s) 351
BstSLI GKGCMC 1 cut(s) 162
BstUI CGCG 2 cut(s) 12, 707
BstX2I RGATCY 1 cut(s) 331
BstYI RGATCY 1 cut(s) 331
BtgI CCRYGG 1 cut(s) 184
BtrI CACGTC 1 cut(s) 32
BtsCI GGATG 4 cut(s) 616, 694, 757, 1009
BtsI GCAGTG 1 cut(s) 997
BtsIMutI CAGTG 1 cut(s) 997
BveI ACCTGC 2 cut(s) 493, 586
Cac8I GCNNGC 1 cut(s) 162
CaiI CAGNNNCTG 1 cut(s) 350
Cfr13I GGNCC 2 cut(s) 181, 454
CviAII CATG 2 cut(s) 185, 272
DdeI CTNAG 2 cut(s) 399, 867
DpnI GATC 4 cut(s) 176, 333, 704, 965
DpnII GATC 4 cut(s) 174, 331, 702, 963
Eam1104I CTCTTC 3 cut(s) 246, 689, 968
EarI CTCTTC 3 cut(s) 246, 689, 968
EciI GGCGGA 1 cut(s) 809
Ecl136II GAGCTC 1 cut(s) 1149
Eco130I CCWWGG 2 cut(s) 184, 199
Eco24I GRGCYC 3 cut(s) 591, 732, 1151
Eco47I GGWCC 2 cut(s) 181, 454
Eco53kI GAGCTC 1 cut(s) 1149
Eco57I CTGAAG 1 cut(s) 114
Eco88I CYCGRG 1 cut(s) 501
EcoICRI GAGCTC 1 cut(s) 1149
EcoRII CCWGG 3 cut(s) 121, 166, 847
EcoT14I CCWWGG 2 cut(s) 184, 199
EcoT38I GRGCYC 3 cut(s) 591, 732, 1151
ErhI CCWWGG 2 cut(s) 184, 199
Esp3I CGTCTC 1 cut(s) 498
FaeI CATG 2 cut(s) 188, 275
FaiI YATR 9 cut(s) 54, 107, 109, 111, 186, 273, 773, 827, 1144
FaqI GGGAC 2 cut(s) 30, 305
FatI CATG 2 cut(s) 184, 271
FbaI TGATCA 1 cut(s) 174
FblI GTMKAC 1 cut(s) 879
FokI GGATG 4 cut(s) 623, 701, 764, 1016
FriOI GRGCYC 3 cut(s) 591, 732, 1151
FspBI CTAG 1 cut(s) 1043
GsuI CTGGAG 1 cut(s) 870
HapII CCGG 1 cut(s) 1155
Hin1II CATG 2 cut(s) 188, 275
HincII GTYRAC 1 cut(s) 880
HindII GTYRAC 1 cut(s) 880
HpaII CCGG 1 cut(s) 1155
HphI GGTGA 2 cut(s) 611, 712
Hpy166II GTNNAC 2 cut(s) 160, 880
Hpy188III TCNNGA 6 cut(s) 608, 668, 815, 967, 1043, 1155
Hpy8I GTNNAC 2 cut(s) 160, 880
HpyAV CCTTC 4 cut(s) 76, 445, 450, 784
HpyCH4IV ACGT 3 cut(s) 31, 463, 982
HpyCH4V TGCA 5 cut(s) 160, 353, 722, 1014, 1022
HpyF10VI GCNNNNNNNGC 8 cut(s) 350, 353, 392, 401, 494, 497, 641, 1121
HpyF3I CTNAG 2 cut(s) 399, 867
HpySE526I ACGT 3 cut(s) 31, 463, 982
Hsp92II CATG 2 cut(s) 188, 275
Kpn2I TCCGGA 1 cut(s) 1154
Ksp22I TGATCA 1 cut(s) 174
Kzo9I GATC 4 cut(s) 174, 331, 702, 963
LguI GCTCTTC 1 cut(s) 968
LmnI GCTCC 5 cut(s) 216, 472, 565, 1146, 1154
LweI GCATC 1 cut(s) 601
MaeI CTAG 1 cut(s) 1043
MaeII ACGT 3 cut(s) 31, 463, 982
MalI GATC 4 cut(s) 176, 333, 704, 965
MboI GATC 4 cut(s) 174, 331, 702, 963
MboII GAAGA 6 cut(s) 98, 233, 706, 932, 985, 1076
MflI RGATCY 1 cut(s) 331
MhlI GDGCHC 5 cut(s) 162, 469, 591, 732, 1151
MluCI AATT 6 cut(s) 77, 250, 267, 592, 756, 1015
MluI ACGCGT 1 cut(s) 10
MmeI TCCRAC 1 cut(s) 590
MroI TCCGGA 1 cut(s) 1154
MroXI GAANNNNTTC 1 cut(s) 234
MseI TTAA 1 cut(s) 1079
MspA1I CMGCKG 2 cut(s) 347, 1075
MspI CCGG 1 cut(s) 1155
MspR9I CCNGG 3 cut(s) 123, 168, 849
MvaI CCWGG 3 cut(s) 123, 168, 849
MvnI CGCG 2 cut(s) 12, 707
MwoI GCNNNNNNNGC 8 cut(s) 350, 353, 392, 401, 494, 497, 641, 1121
NcoI CCATGG 1 cut(s) 184
NdeII GATC 4 cut(s) 174, 331, 702, 963
NlaIII CATG 2 cut(s) 188, 275
NlaIV GGNNCC 3 cut(s) 182, 218, 438
NspV TTCGAA 1 cut(s) 443
PciSI GCTCTTC 1 cut(s) 968
PdmI GAANNNNTTC 1 cut(s) 234
Ppu21I YACGTR 1 cut(s) 464
Psp124BI GAGCTC 1 cut(s) 1151
Psp1406I AACGTT 1 cut(s) 982
Psp6I CCWGG 3 cut(s) 121, 166, 847
PspGI CCWGG 3 cut(s) 121, 166, 847
PspN4I GGNNCC 3 cut(s) 182, 218, 438
PspPI GGNCC 2 cut(s) 181, 454
PstI CTGCAG 1 cut(s) 355
PstNI CAGNNNCTG 1 cut(s) 350
PsuI RGATCY 1 cut(s) 331
PvuII CAGCTG 1 cut(s) 1075
SacI GAGCTC 1 cut(s) 1151
SalI GTCGAC 1 cut(s) 878
SapI GCTCTTC 1 cut(s) 968
SaqAI TTAA 1 cut(s) 1079
Sau3AI GATC 4 cut(s) 174, 331, 702, 963
Sau96I GGNCC 2 cut(s) 181, 454
ScrFI CCNGG 3 cut(s) 123, 168, 849
SduI GDGCHC 5 cut(s) 162, 469, 591, 732, 1151
SfaNI GCATC 1 cut(s) 601
SfcI CTRYAG 1 cut(s) 351
SfuI TTCGAA 1 cut(s) 443
SinI GGWCC 2 cut(s) 181, 454
Sse9I AATT 6 cut(s) 77, 250, 267, 592, 756, 1015
SsiI CCGC 3 cut(s) 347, 386, 794
SspMI CTAG 1 cut(s) 1043
SstI GAGCTC 1 cut(s) 1151
StyD4I CCNGG 3 cut(s) 121, 166, 847
StyI CCWWGG 2 cut(s) 184, 199
TaiI ACGT 3 cut(s) 34, 466, 985
TaqI TCGA 4 cut(s) 443, 585, 879, 933
TasI AATT 6 cut(s) 77, 250, 267, 592, 756, 1015
TauI GCSGC 2 cut(s) 350, 389
Tru1I TTAA 1 cut(s) 1079
Tru9I TTAA 1 cut(s) 1079
TscAI CASTG 1 cut(s) 997
TspDTI ATGAA 5 cut(s) 260, 768, 795, 986, 1077
TspRI CASTG 1 cut(s) 997
VneI GTGCAC 1 cut(s) 158
VpaK11BI GGWCC 2 cut(s) 181, 454
XapI RAATTY 2 cut(s) 267, 756
XbaI TCTAGA 1 cut(s) 1042
XmiI GTMKAC 1 cut(s) 879
XmnI GAANNNNTTC 1 cut(s) 234
XspI CTAG 1 cut(s) 1043
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.