AT5G05350

Protein of unknown function (DUF2985)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012162
Physical Location & Seq
Forward (+)
1585249 .. 1587517
2269 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G05350.1

Sequence Viewer

Length: 1581 bp
ATGGGTTCAGTTGATGAGGTTGAGATATCTAAGACTAGGGTTTCATGTCCTCCTGCTCTCTCAACATCTCATAAGATTCTCATCAGTGAAGAGAAACCTAGGAGATGGTCCGAGAGTTCGTTGCCTGATGTCTCTAATAGGATTAAGCTTTTGAAATTTGGTTCCCCTTCTGCTAGACTCAAGAGAATGGCTGAGGAAAGAGATGAAGTTTCACGCTCTGTCAACAGCTCAAGCTCTAGCAGCCACGATGTGGTGTCTTCCCGGAAAATCGAGTGGGCTTGTTTGATGAAAATGGGTAAGCAATGGCTACAGAACCCGTTGAACATGGTTATGTTTTTGTGGATCTTTGTGGTTGCTGTCTCCGGTGCGATTCTATTCATGGTTATGACTGGTATGTTGAACCATGCTTTGCCCAAGAAATCACAGAGAGATGTCTGGTTTGAAGTTAACAACCAAATCCTCAACGCATTGTTTACTCTGATGTGTCTGTATCAACACCCGAAACGGTTTTACCACCTTGTGCTTCTCTGTAGATGGAAGCAAGACGATGTTACAACGCTTAGGAAGATATTCTGCAAGAACGGGACTTACAAGCCAAACGAATGGATACACATGATGGTTGTTGTTCTTTTGCTTCACTTGAACTGTTTTGCTCAGTATGCGCTTTGCGGTCTTAACTTAGGCTATAGAAGATCCGAGAGACCTGCCATTGGTGTAGCTATATGTATATCTTTTGCAATAGCAGCTCCTACGGCTGCGGGTTTGTACACTATTCTTAGTCCACTTGGGAAAGATTATGATCCTCAAGGAGATGAGGAGAATCAAGTGCAGGCTGTGGGAGAAGGACCGGTTAGTAATCGCAAGTTATCTCTTGAAAGGAGATATTCATTTGCTTCTAATGATGTGAGTAATCCTGAATGGAGAGAGGGAGTTCTTGACATATGGGAAGATATTTCATTGGCTTATCTCTCTCTGTTCTGCACTTTTTGTTTGTTTGGTTGGAACATGGAGAGGATTGGGTTTGGGAATATGTATGTGCACATTGCGACTTTTGTTCTCTTCTGTTTAGCTCCTTTCTTTATATTCAACTTGGCTGCTATAAACATTGATAACGAGATGGTAAGGGATGCACTTGGATATACTGGGATTGTGCTTTGCTTGTTTGGTTTGCTCTATGGTGGTTTTTGGAGAATACAGATGAGGAAAAGATTCAAGTTGCCGGGCTATAACATCTGTTGCGGGAGACCCGCGATTGCTGATTGTACACTCTGGTTGTTCTGTTGTTGGTGCTCTTTAGCTCAAGAAGTCCGGACCGCGAATTCTTATGAAATAGTGGAGGATAAATTTTGCAAAAGAAGAGAAGAAAATAGTAAGATTGATGATGAGGTTGTCGTATCTGCATTGCCTCGTGAAGACGGTGTGTTTGATCCGAGTTGCTTGCCTAAGAAGATGACCACGACGATGATAGCTAGCTCTACTCTTAGCCCGAGCAGACAGAAAGATGAAACTTGCTTGGGAGAGAAAGGTGATGAAGCTCTAAGTCCACCTTCTCCTCCATTCATACACAGAGAAGCTAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2985 PLAC8

Protein Analysis

526

Amino Acids

59.68

Weight (kDa)

8.14

Isoelectric Point (pI)

55.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2985 PF11204 101 - 179 7.6e-31 Protein of unknown function (DUF2985)
PLAC8 PF04749 307 - 437 3.8e-19 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012162)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10980 AT5G05350
fragaria_vesca FvH4_7g25461
malus_domestica MD01G1161000.v1.1 MD07G1229000.v1.1
prunus_persica Prupe.2G259100_v2.0.a1
pyrus_communis pycom01g17680 pycom07g20720
rosa_chinensis RchiOBHm_Chr1g0371431
rosa_laevigata RLG00000026963
rosa_multiflora Rmu_sc0000809.1_g000023 Rmu_sc0017835.1_g000001
rosa_roxburghii Rroxscaffold_4G00285690
rosa_rugosa Rorug01G0363900
rosa_samantha Rh1AG374100 Rh1BG337500 Rh1CG350800 Rh1DG368500
rosa_wichuraiana Rw1G032900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 712
AccB7I CCANNNNNTGG 1 cut(s) 250
AccII CGCG 2 cut(s) 1250, 1316
AccIII TCCGGA 1 cut(s) 1308
AciI CCGC 5 cut(s) 669, 758, 1239, 1248, 1314
AclWI GGATC 4 cut(s) 350, 687, 794, 1421
AcsI RAATTY 3 cut(s) 155, 1318, 1343
AdeI CACNNNGTG 2 cut(s) 250, 520
AfaI GTAC 2 cut(s) 767, 1264
AfiI CCNNNNNNNGG 2 cut(s) 250, 710
AgeI ACCGGT 1 cut(s) 847
AgsI TTSAA 8 cut(s) 154, 322, 400, 443, 643, 875, 1087, 1213
Alw21I GWGCWC 2 cut(s) 1041, 1292
Alw26I GTCTC 4 cut(s) 136, 364, 694, 1237
Alw44I GTGCAC 1 cut(s) 1037
AlwI GGATC 4 cut(s) 350, 687, 794, 1421
Ama87I CYCGRG 1 cut(s) 1486
Aor13HI TCCGGA 1 cut(s) 1308
ApaLI GTGCAC 1 cut(s) 1037
ApeKI GCWGC 4 cut(s) 240, 743, 755, 1094
ApoI RAATTY 3 cut(s) 155, 1318, 1343
ArsI GACNNNNNNTTYG 2 cut(s) 1406, 1438
AsiGI ACCGGT 1 cut(s) 847
Asp700I GAANNNNTTC 2 cut(s) 569, 1208
AspA2I CCTAGG 1 cut(s) 98
AspLEI GCGC 1 cut(s) 664
AspS9I GGNCC 3 cut(s) 108, 845, 1311
AsuC2I CCSGG 2 cut(s) 262, 1221
AsuHPI GGTGA 1 cut(s) 1538
AsuNHI GCTAGC 1 cut(s) 1469
AvaI CYCGRG 1 cut(s) 1486
AvaII GGWCC 3 cut(s) 108, 845, 1311
AvrII CCTAGG 1 cut(s) 98
BaeGI GKGCMC 1 cut(s) 1041
BauI CACGAG 1 cut(s) 1407
BbsI GAAGAC 2 cut(s) 249, 1419
Bbv12I GWGCWC 2 cut(s) 1041, 1292
BbvCI CCTCAGC 1 cut(s) 192
BbvI GCAGC 4 cut(s) 252, 742, 755, 1081
BccI CCATC 4 cut(s) 99, 528, 610, 1111
BceAI ACGGC 1 cut(s) 768
BcgI CGANNNNNNTGC 4 cut(s) 686, 720, 1420, 1454
BciVI GTATCC 1 cut(s) 600
BcnI CCSGG 2 cut(s) 262, 1221
BcoDI GTCTC 4 cut(s) 136, 364, 694, 1237
BfaI CTAG 6 cut(s) 36, 99, 174, 237, 1470, 1575
BfmI CTRYAG 3 cut(s) 308, 529, 685
BfuAI ACCTGC 1 cut(s) 712
BfuI GTATCC 1 cut(s) 600
BisI GCNGC 4 cut(s) 241, 744, 756, 1095
BlnI CCTAGG 1 cut(s) 98
BlsI GCNGC 4 cut(s) 242, 745, 757, 1096
Bme1390I CCNGG 2 cut(s) 262, 1221
Bme18I GGWCC 3 cut(s) 108, 845, 1311
BmeT110I CYCGRG 1 cut(s) 1486
BmgT120I GGNCC 3 cut(s) 108, 845, 1311
BmiI GGNNCC 1 cut(s) 163
BmrFI CCNGG 2 cut(s) 262, 1221
BmrI ACTGGG 1 cut(s) 1152
BmsI GCATC 1 cut(s) 1117
BmtI GCTAGC 1 cut(s) 1473
BmuI ACTGGG 1 cut(s) 1152
BpiI GAAGAC 2 cut(s) 249, 1419
Bpu10I CCTNAGC 2 cut(s) 192, 560
BpuEI CTTGAG 4 cut(s) 164, 214, 789, 1284
BpuMI CCSGG 2 cut(s) 262, 1221
BsaBI GATNNNNATC 2 cut(s) 80, 798
BsaI GGTCTC 2 cut(s) 694, 1237
BsaJI CCNNGG 1 cut(s) 98
BsaWI WCCGGW 3 cut(s) 362, 847, 1308
Bsc4I CCNNNNNNNGG 2 cut(s) 250, 710
Bse118I RCCGGY 1 cut(s) 847
Bse1I ACTGG 2 cut(s) 394, 1147
Bse3DI GCAATG 3 cut(s) 308, 1041, 1400
Bse8I GATNNNNATC 2 cut(s) 80, 798
BseAI TCCGGA 1 cut(s) 1308
BseDI CCNNGG 1 cut(s) 98
BseGI GGATG 1 cut(s) 1132
BseJI GATNNNNATC 2 cut(s) 80, 798
BseLI CCNNNNNNNGG 2 cut(s) 250, 710
BseMI GCAATG 3 cut(s) 308, 1041, 1400
BseMII CTCAG 2 cut(s) 183, 668
BseNI ACTGG 2 cut(s) 394, 1147
BseRI GAGGAG 2 cut(s) 830, 1542
BseSI GKGCMC 1 cut(s) 1041
BseXI GCAGC 4 cut(s) 252, 742, 755, 1081
BsgI GTGCAG 2 cut(s) 848, 964
Bsh1236I CGCG 2 cut(s) 1250, 1316
BshTI ACCGGT 1 cut(s) 847
BsiHKAI GWGCWC 2 cut(s) 1041, 1292
BsiHKCI CYCGRG 1 cut(s) 1486
BsiSI CCGG 5 cut(s) 262, 363, 848, 1220, 1309
BslFI GGGAC 1 cut(s) 598
BslI CCNNNNNNNGG 2 cut(s) 250, 710
BsmAI GTCTC 4 cut(s) 136, 364, 694, 1237
BsmFI GGGAC 1 cut(s) 598
Bso31I GGTCTC 2 cut(s) 694, 1237
BsoBI CYCGRG 1 cut(s) 1486
Bsp1286I GDGCHC 2 cut(s) 1041, 1292
Bsp13I TCCGGA 1 cut(s) 1308
Bsp1407I TGTACA 2 cut(s) 765, 1262
Bsp143I GATC 4 cut(s) 342, 692, 799, 1426
BspACI CCGC 5 cut(s) 669, 758, 1239, 1248, 1314
BspCNI CTCAG 2 cut(s) 184, 667
BspEI TCCGGA 1 cut(s) 1308
BspFNI CGCG 2 cut(s) 1250, 1316
BspLI GGNNCC 1 cut(s) 163
BspMI ACCTGC 1 cut(s) 712
BspOI GCTAGC 1 cut(s) 1473
BspPI GGATC 4 cut(s) 350, 687, 794, 1421
BspTNI GGTCTC 2 cut(s) 694, 1237
BsrDI GCAATG 3 cut(s) 308, 1041, 1400
BsrFI RCCGGY 1 cut(s) 847
BsrGI TGTACA 2 cut(s) 765, 1262
BsrI ACTGG 2 cut(s) 394, 1147
BssAI RCCGGY 1 cut(s) 847
BssECI CCNNGG 1 cut(s) 98
BssMI GATC 4 cut(s) 342, 692, 799, 1426
BssSI CACGAG 1 cut(s) 1407
BssT1I CCWWGG 1 cut(s) 98
Bst2BI CACGAG 1 cut(s) 1407
Bst4CI ACNGT 3 cut(s) 507, 647, 1418
Bst6I CTCTTC 3 cut(s) 84, 1064, 1351
BstAUI TGTACA 2 cut(s) 765, 1262
BstC8I GCNNGC 3 cut(s) 831, 1439, 1471
BstDEI CTNAG 9 cut(s) 30, 192, 560, 654, 679, 776, 1443, 1481, 1538
BstF5I GGATG 1 cut(s) 1132
BstFNI CGCG 2 cut(s) 1250, 1316
BstHHI GCGC 1 cut(s) 664
BstKTI GATC 4 cut(s) 345, 695, 802, 1429
BstMAI GTCTC 4 cut(s) 136, 364, 694, 1237
BstMBI GATC 4 cut(s) 342, 692, 799, 1426
BstMWI GCNNNNNNNGC 4 cut(s) 240, 659, 743, 752
BstSCI CCNGG 2 cut(s) 260, 1219
BstSFI CTRYAG 3 cut(s) 308, 529, 685
BstSLI GKGCMC 1 cut(s) 1041
BstUI CGCG 2 cut(s) 1250, 1316
BstV1I GCAGC 4 cut(s) 252, 742, 755, 1081
BstV2I GAAGAC 2 cut(s) 249, 1419
BstX2I RGATCY 2 cut(s) 342, 692
BstXI CCANNNNNNTGG 1 cut(s) 603
BstYI RGATCY 2 cut(s) 342, 692
BsuI GTATCC 1 cut(s) 600
BtsCI GGATG 1 cut(s) 1132
BtsIMutI CAGTG 1 cut(s) 91
BveI ACCTGC 1 cut(s) 712
Cac8I GCNNGC 3 cut(s) 831, 1439, 1471
CfoI GCGC 1 cut(s) 664
Cfr10I RCCGGY 1 cut(s) 847
Cfr13I GGNCC 3 cut(s) 108, 845, 1311
CpoI CGGWCCG 1 cut(s) 1311
Csp6I GTAC 2 cut(s) 766, 1263
CspAI ACCGGT 1 cut(s) 847
CspI CGGWCCG 1 cut(s) 1311
CviAII CATG 6 cut(s) 45, 325, 379, 404, 613, 1006
CviQI GTAC 2 cut(s) 766, 1263
DdeI CTNAG 9 cut(s) 30, 192, 560, 654, 679, 776, 1443, 1481, 1538
DpnI GATC 4 cut(s) 344, 694, 801, 1428
DpnII GATC 4 cut(s) 342, 692, 799, 1426
DraIII CACNNNGTG 2 cut(s) 250, 520
Eam1104I CTCTTC 3 cut(s) 84, 1064, 1351
EarI CTCTTC 3 cut(s) 84, 1064, 1351
Eco130I CCWWGG 1 cut(s) 98
Eco31I GGTCTC 2 cut(s) 694, 1237
Eco32I GATATC 1 cut(s) 27
Eco47I GGWCC 3 cut(s) 108, 845, 1311
Eco88I CYCGRG 1 cut(s) 1486
EcoRI GAATTC 1 cut(s) 1318
EcoRV GATATC 1 cut(s) 27
EcoT14I CCWWGG 1 cut(s) 98
ErhI CCWWGG 1 cut(s) 98
FaeI CATG 6 cut(s) 48, 328, 382, 407, 616, 1009
FalI AAGNNNNNCTT 2 cut(s) 1531, 1563
FaqI GGGAC 1 cut(s) 598
FatI CATG 6 cut(s) 44, 324, 378, 403, 612, 1005
FauI CCCGC 3 cut(s) 751, 1232, 1255
FauNDI CATATG 1 cut(s) 941
Fnu4HI GCNGC 4 cut(s) 241, 744, 756, 1095
FokI GGATG 1 cut(s) 1139
Fsp4HI GCNGC 4 cut(s) 241, 744, 756, 1095
FspBI CTAG 6 cut(s) 36, 99, 174, 237, 1470, 1575
GlaI GCGC 1 cut(s) 663
GluI GCNGC 4 cut(s) 241, 744, 756, 1095
HapII CCGG 5 cut(s) 262, 363, 848, 1220, 1309
HhaI GCGC 1 cut(s) 664
Hin1II CATG 6 cut(s) 48, 328, 382, 407, 616, 1009
Hin6I GCGC 1 cut(s) 662
HinP1I GCGC 1 cut(s) 662
HincII GTYRAC 2 cut(s) 223, 448
HindII GTYRAC 2 cut(s) 223, 448
HindIII AAGCTT 1 cut(s) 146
HinfI GANTC 5 cut(s) 76, 177, 370, 820, 1209
HpaI GTTAAC 1 cut(s) 448
HpaII CCGG 5 cut(s) 262, 363, 848, 1220, 1309
HphI GGTGA 1 cut(s) 1538
Hpy166II GTNNAC 8 cut(s) 223, 448, 474, 768, 782, 1039, 1265, 1544
Hpy188I TCNGA 4 cut(s) 112, 480, 697, 1431
Hpy188III TCNNGA 7 cut(s) 181, 872, 914, 935, 1301, 1309, 1409
Hpy8I GTNNAC 8 cut(s) 223, 448, 474, 768, 782, 1039, 1265, 1544
Hpy99I CGWCG 1 cut(s) 1462
HpyAV CCTTC 3 cut(s) 177, 836, 1557
HpyCH4III ACNGT 3 cut(s) 507, 647, 1418
HpyCH4V TGCA 8 cut(s) 576, 737, 829, 981, 1039, 1130, 1350, 1400
HpyF10VI GCNNNNNNNGC 4 cut(s) 240, 659, 743, 752
HpyF3I CTNAG 9 cut(s) 30, 192, 560, 654, 679, 776, 1443, 1481, 1538
Hsp92II CATG 6 cut(s) 48, 328, 382, 407, 616, 1009
HspAI GCGC 1 cut(s) 662
Kpn2I TCCGGA 1 cut(s) 1308
KspAI GTTAAC 1 cut(s) 448
Kzo9I GATC 4 cut(s) 342, 692, 799, 1426
LmnI GCTCC 2 cut(s) 751, 1075
Lsp1109I GCAGC 4 cut(s) 252, 742, 755, 1081
LweI GCATC 1 cut(s) 1117
MaeI CTAG 6 cut(s) 36, 99, 174, 237, 1470, 1575
MaeIII GTNAC 1 cut(s) 550
MalI GATC 4 cut(s) 344, 694, 801, 1428
MboI GATC 4 cut(s) 342, 692, 799, 1426
MflI RGATCY 2 cut(s) 342, 692
MhlI GDGCHC 2 cut(s) 1041, 1292
MluCI AATT 3 cut(s) 155, 1318, 1343
MlyI GAGTC 1 cut(s) 171
MmeI TCCRAC 1 cut(s) 980
MroI TCCGGA 1 cut(s) 1308
MroXI GAANNNNTTC 2 cut(s) 569, 1208
MseI TTAA 3 cut(s) 144, 447, 675
MslI CAYNNNNRTG 3 cut(s) 329, 383, 1460
MspI CCGG 5 cut(s) 262, 363, 848, 1220, 1309
MspR9I CCNGG 2 cut(s) 262, 1221
MvnI CGCG 2 cut(s) 1250, 1316
MwoI GCNNNNNNNGC 4 cut(s) 240, 659, 743, 752
NciI CCSGG 2 cut(s) 262, 1221
NdeI CATATG 1 cut(s) 941
NdeII GATC 4 cut(s) 342, 692, 799, 1426
NheI GCTAGC 1 cut(s) 1469
NlaIII CATG 6 cut(s) 48, 328, 382, 407, 616, 1009
NlaIV GGNNCC 1 cut(s) 163
PdmI GAANNNNTTC 2 cut(s) 569, 1208
PfeI GAWTC 4 cut(s) 76, 370, 820, 1209
PflMI CCANNNNNTGG 1 cut(s) 250
PfoI TCCNGGA 1 cut(s) 260
PinAI ACCGGT 1 cut(s) 847
PkrI GCNGC 4 cut(s) 242, 745, 757, 1096
PleI GAGTC 1 cut(s) 171
PpsI GAGTC 1 cut(s) 171
PspN4I GGNNCC 1 cut(s) 163
PspPI GGNCC 3 cut(s) 108, 845, 1311
PsrI GAACNNNNNNTAC 2 cut(s) 572, 604
PsuI RGATCY 2 cut(s) 342, 692
RsaI GTAC 2 cut(s) 767, 1264
RsaNI GTAC 2 cut(s) 766, 1263
RseI CAYNNNNRTG 3 cut(s) 329, 383, 1460
Rsr2I CGGWCCG 1 cut(s) 1311
RsrII CGGWCCG 1 cut(s) 1311
SaqAI TTAA 3 cut(s) 144, 447, 675
SatI GCNGC 4 cut(s) 241, 744, 756, 1095
Sau3AI GATC 4 cut(s) 342, 692, 799, 1426
Sau96I GGNCC 3 cut(s) 108, 845, 1311
SchI GAGTC 1 cut(s) 171
ScrFI CCNGG 2 cut(s) 262, 1221
SduI GDGCHC 2 cut(s) 1041, 1292
SfaNI GCATC 1 cut(s) 1117
SfcI CTRYAG 3 cut(s) 308, 529, 685
SinI GGWCC 3 cut(s) 108, 845, 1311
SmiMI CAYNNNNRTG 3 cut(s) 329, 383, 1460
SmlI CTYRAG 4 cut(s) 179, 229, 804, 1299
SmoI CTYRAG 4 cut(s) 179, 229, 804, 1299
Sse9I AATT 3 cut(s) 155, 1318, 1343
SsiI CCGC 5 cut(s) 669, 758, 1239, 1248, 1314
SspMI CTAG 6 cut(s) 36, 99, 174, 237, 1470, 1575
StyD4I CCNGG 2 cut(s) 260, 1219
StyI CCWWGG 1 cut(s) 98
TaaI ACNGT 3 cut(s) 507, 647, 1418
TaqI TCGA 1 cut(s) 270
TasI AATT 3 cut(s) 155, 1318, 1343
TatI WGTACW 2 cut(s) 765, 1262
TfiI GAWTC 4 cut(s) 76, 370, 820, 1209
Tru1I TTAA 3 cut(s) 144, 447, 675
Tru9I TTAA 3 cut(s) 144, 447, 675
TscAI CASTG 1 cut(s) 91
TseI GCWGC 4 cut(s) 240, 743, 755, 1094
TspRI CASTG 1 cut(s) 91
Van91I CCANNNNNTGG 1 cut(s) 250
VneI GTGCAC 1 cut(s) 1037
VpaK11BI GGWCC 3 cut(s) 108, 845, 1311
XapI RAATTY 3 cut(s) 155, 1318, 1343
XmaJI CCTAGG 1 cut(s) 98
XmnI GAANNNNTTC 2 cut(s) 569, 1208
XspI CTAG 6 cut(s) 36, 99, 174, 237, 1470, 1575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.