pycom07g20720

Protein of unknown function (DUF2985)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0012162
Physical Location & Seq
Reverse (-)
22714658 .. 22716316
1659 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g20720.1

Sequence Viewer

Length: 1659 bp
ATGGTTTCGACTCAAAATGGAAACCACAAGGAGGAAATTGAAGAATCGAATGGTAGTGAAGTGAAGGGTCAAATCCCCCTTGACGTTTCAGCATCCCCAAAGACTTTTCTAGTCAATGAGAAGTCTCCGAAAAGGTTCCCTCCTGATGCTCCTCCTAATAGAGTTAGGTTCCTCAAATTTGGTTCTGCATCTGCCAGGTTCAAGCGGTTGGCTGAGGAGAGAGATGAAATGTCACGATCTGTGGCTTCAACCGGTCATGGCTTCAAAGAACGAATCAATGGGGTTTTTTCTAAGAAAATTGATTGGGTTTCACTCCTGAAAATGGGCAAAGTATGGATTAGAAATCCAATGAACATGGCTCTTTTTGCATGGATTGTCTGTGTTGCTATTTCGGGTGCAATTTTGTTTCTTGTCATGACTGGAATGTTGAACAAGGCAATACCCAAGAAATCCCAGAGAGATACATGGTTTGAAGTCAATAATCAAATTCTCAATGCACTTTTTACGCTTATGTGTTTGTATAACCATCCGAAGCGGTTCCACCACCTTGTACTTCTGTGTAGATGGAAACCAGAAGACATTTCCAGACTTCGAAAGATTTACTGTAAAAATGGGACTTATAAGCCACATGAATGGGCCCACATGATGGTTGTTGTTGCCCTGCTTCATCTGAATTGCTTTGCTCAGTATGCACTTTGTGGTTTAAACCTGGGTTACAGAAGAGCTGAACGGCCACCCATAGGAGTTGCAGTATGTATATCTTTTGCTATTGGTGCACCTGCATTTGCTGGTATATACAACATTGTCAGCCCCCTTGGGAAGGATTATGATTCAGATGTGGATGAGGAAGCACAGGTTCAGATTGGAACTGGTGAAGGAGCAGAAGCATTAAGGTTAAAATCATTTGAGAGAAGATATTCTTTTGCATTTAGGACTGAACAACGAGTCGTTGAGAATAGACCACAATGGAGCGGAGGAGTACTTGACATTTGGGATGATATTTCTATTGCGTATGTTTCACTCTTTTGTAGTTTTTGTGTATTTGGGTGGAATATGGAGAGGCTTGGATTTGGGAACATATATGTTCATATTATGACATTTGTTCTGTTTTGCATGGCTCCGTTCTGGATTTTTAACTTGGCTGCTGTTAATATCGACAATGAAACTGTTCGGGAGGCTCTTGGAGTTACTGGCCTTGTTCTATGTCTATTTGGTTTGCTCTATGGTGGCTTTTGGAGGATCCAAATGAGAAAGAGATTCAATTTGCCATCTTATAACTTCTGTTTTGGTGAACCTGCGCTCTCTGATTGCACGTTGTGGCTTTGCTGTTGTTGGTGCTCTCTTGCTCAGGAAGTGAGGACCGGGAATTCCTATGACATTGTGGAAGAAAAGTTCTATAGAAAACAACCAGAAGGTGGATGCCCTTCACCAATTTCACCTTTGCCTCGTGAAGATGGATCGGTTGGGTTTAGATCTGGCCCGAATTGTCCTCTTGGGAACTACTCAAGCCCAACTAAGAATGGCATCGGTGGTTCTATGAGTCAAAGCAGACTTCCAGCGGGATTTCATAGTCCAGTGAAAGAAGAGTCCCATGTAAGAGGTAAGGATGAAACTATGACCCCACCTGCATCTTCAATGATACAAAGGGAAGAAACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2985 PLAC8

Protein Analysis

553

Amino Acids

62.23

Weight (kDa)

8.74

Isoelectric Point (pI)

46.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2985 PF11204 112 - 189 2.9e-30 Protein of unknown function (DUF2985)
PLAC8 PF04749 323 - 453 2.3e-19 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012162)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10980 AT5G05350
fragaria_vesca FvH4_7g25461
malus_domestica MD01G1161000.v1.1 MD07G1229000.v1.1
prunus_persica Prupe.2G259100_v2.0.a1
pyrus_communis pycom01g17680 pycom07g20720
rosa_chinensis RchiOBHm_Chr1g0371431
rosa_laevigata RLG00000026963
rosa_multiflora Rmu_sc0000809.1_g000023 Rmu_sc0017835.1_g000001
rosa_roxburghii Rroxscaffold_4G00285690
rosa_rugosa Rorug01G0363900
rosa_samantha Rh1AG374100 Rh1BG337500 Rh1CG350800 Rh1DG368500
rosa_wichuraiana Rw1G032900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 621, 1275
AarI CACCTGC 2 cut(s) 787, 1633
Acc36I ACCTGC 3 cut(s) 787, 1303, 1633
AccB7I CCANNNNNTGG 2 cut(s) 646, 1415
AccBSI CCGCTC 1 cut(s) 972
AciI CCGC 4 cut(s) 205, 535, 972, 1559
AclWI GGATC 3 cut(s) 1234, 1247, 1465
AcoI YGGCCR 1 cut(s) 731
AcsI RAATTY 3 cut(s) 176, 486, 1366
AdeI CACNNNGTG 2 cut(s) 698, 1317
AfaI GTAC 2 cut(s) 552, 981
AfiI CCNNNNNNNGG 6 cut(s) 31, 322, 646, 740, 820, 1415
AgeI ACCGGT 1 cut(s) 251
AgsI TTSAA 8 cut(s) 41, 202, 249, 265, 430, 473, 1261, 1635
AjnI CCWGG 2 cut(s) 194, 708
AloI GAACNNNNNNTCC 2 cut(s) 1376, 1408
AluBI AGCT 1 cut(s) 725
AluI AGCT 1 cut(s) 725
Alw21I GWGCWC 2 cut(s) 778, 1340
Alw26I GTCTC 1 cut(s) 129
Alw44I GTGCAC 1 cut(s) 774
AlwI GGATC 3 cut(s) 1234, 1247, 1465
AoxI GGCC 4 cut(s) 636, 731, 1192, 1477
ApaI GGGCCC 1 cut(s) 640
ApaLI GTGCAC 1 cut(s) 774
ApeKI GCWGC 1 cut(s) 1142
ApoI RAATTY 3 cut(s) 176, 486, 1366
AsiGI ACCGGT 1 cut(s) 251
Asp700I GAANNNNTTC 4 cut(s) 134, 536, 916, 1167
AspLEI GCGC 1 cut(s) 1300
AspS9I GGNCC 4 cut(s) 636, 637, 1359, 1478
AsuC2I CCSGG 1 cut(s) 1363
AsuHPI GGTGA 4 cut(s) 884, 1301, 1419, 1428
AsuII TTCGAA 1 cut(s) 592
AvaII GGWCC 1 cut(s) 1359
BaeGI GKGCMC 2 cut(s) 640, 778
BamHI GGATCC 1 cut(s) 1239
BanII GRGCYC 1 cut(s) 640
BauI CACGAG 1 cut(s) 1446
BbsI GAAGAC 1 cut(s) 582
Bbv12I GWGCWC 2 cut(s) 778, 1340
BbvCI CCTCAGC 1 cut(s) 213
BbvI GCAGC 1 cut(s) 1129
BccI CCATC 5 cut(s) 534, 558, 640, 1276, 1448
BceAI ACGGC 1 cut(s) 746
BciT130I CCWGG 2 cut(s) 196, 710
BcnI CCSGG 1 cut(s) 1363
BcoDI GTCTC 1 cut(s) 129
BfaI CTAG 1 cut(s) 110
BfmI CTRYAG 1 cut(s) 1396
BfuAI ACCTGC 3 cut(s) 787, 1303, 1633
BglII AGATCT 1 cut(s) 1472
BisI GCNGC 1 cut(s) 1143
BlsI GCNGC 1 cut(s) 1144
BmcAI AGTACT 1 cut(s) 981
Bme1390I CCNGG 3 cut(s) 196, 710, 1363
Bme18I GGWCC 1 cut(s) 1359
BmgT120I GGNCC 4 cut(s) 636, 637, 1359, 1478
BmiI GGNNCC 6 cut(s) 137, 170, 539, 638, 1119, 1241
BmrFI CCNGG 3 cut(s) 196, 710, 1363
BmsI GCATC 6 cut(s) 101, 136, 197, 1409, 1533, 1637
BpiI GAAGAC 1 cut(s) 582
Bpu10I CCTNAGC 2 cut(s) 213, 1347
Bpu14I TTCGAA 1 cut(s) 592
BpuEI CTTGAG 1 cut(s) 1489
BpuMI CCSGG 1 cut(s) 1363
BsaJI CCNNGG 2 cut(s) 709, 814
BsaWI WCCGGW 1 cut(s) 251
BsaXI ACNNNNNCTCC 2 cut(s) 735, 765
Bsc4I CCNNNNNNNGG 6 cut(s) 31, 322, 646, 740, 820, 1415
Bse118I RCCGGY 1 cut(s) 251
Bse1I ACTGG 4 cut(s) 424, 874, 1195, 1574
BseBI CCWGG 2 cut(s) 196, 710
BseDI CCNNGG 2 cut(s) 709, 814
BseGI GGATG 6 cut(s) 92, 526, 847, 1000, 1424, 1612
BseLI CCNNNNNNNGG 6 cut(s) 31, 322, 646, 740, 820, 1415
BseMII CTCAG 3 cut(s) 204, 698, 1361
BseNI ACTGG 4 cut(s) 424, 874, 1195, 1574
BseRI GAGGAG 3 cut(s) 141, 230, 990
BseSI GKGCMC 2 cut(s) 640, 778
BseXI GCAGC 1 cut(s) 1129
BshFI GGCC 4 cut(s) 638, 733, 1194, 1479
BshTI ACCGGT 1 cut(s) 251
BsiHKAI GWGCWC 2 cut(s) 778, 1340
BsiSI CCGG 2 cut(s) 252, 1362
BslFI GGGAC 2 cut(s) 628, 1573
BslI CCNNNNNNNGG 6 cut(s) 31, 322, 646, 740, 820, 1415
BsmAI GTCTC 1 cut(s) 129
BsmFI GGGAC 2 cut(s) 628, 1573
BsnI GGCC 4 cut(s) 638, 733, 1194, 1479
Bsp119I TTCGAA 1 cut(s) 592
Bsp120I GGGCCC 1 cut(s) 636
Bsp1286I GDGCHC 3 cut(s) 640, 778, 1340
Bsp143I GATC 4 cut(s) 236, 1239, 1457, 1472
BspACI CCGC 4 cut(s) 205, 535, 972, 1559
BspANI GGCC 4 cut(s) 638, 733, 1194, 1479
BspCNI CTCAG 3 cut(s) 205, 697, 1360
BspHI TCATGA 1 cut(s) 414
BspLI GGNNCC 6 cut(s) 137, 170, 539, 638, 1119, 1241
BspMI ACCTGC 3 cut(s) 787, 1303, 1633
BspPI GGATC 3 cut(s) 1234, 1247, 1465
BspQI GCTCTTC 1 cut(s) 715
BspT104I TTCGAA 1 cut(s) 592
BsrBI CCGCTC 1 cut(s) 972
BsrFI RCCGGY 1 cut(s) 251
BsrI ACTGG 4 cut(s) 424, 874, 1195, 1574
BssAI RCCGGY 1 cut(s) 251
BssECI CCNNGG 2 cut(s) 709, 814
BssMI GATC 4 cut(s) 236, 1239, 1457, 1472
BssSI CACGAG 1 cut(s) 1446
BssT1I CCWWGG 1 cut(s) 814
Bst2BI CACGAG 1 cut(s) 1446
Bst2UI CCWGG 2 cut(s) 196, 710
Bst4CI ACNGT 2 cut(s) 605, 1168
Bst6I CTCTTC 2 cut(s) 715, 1578
BstBI TTCGAA 1 cut(s) 592
BstDEI CTNAG 5 cut(s) 213, 291, 684, 1347, 1515
BstENI CCTNNNNNAGG 1 cut(s) 818
BstF5I GGATG 6 cut(s) 92, 526, 847, 1000, 1424, 1612
BstHHI GCGC 1 cut(s) 1300
BstKTI GATC 4 cut(s) 239, 1242, 1460, 1475
BstMAI GTCTC 1 cut(s) 129
BstMBI GATC 4 cut(s) 236, 1239, 1457, 1472
BstMWI GCNNNNNNNGC 3 cut(s) 365, 689, 773
BstNI CCWGG 2 cut(s) 196, 710
BstSCI CCNGG 3 cut(s) 194, 708, 1361
BstSFI CTRYAG 1 cut(s) 1396
BstSLI GKGCMC 2 cut(s) 640, 778
BstV1I GCAGC 1 cut(s) 1129
BstV2I GAAGAC 1 cut(s) 582
BstX2I RGATCY 2 cut(s) 1239, 1472
BstXI CCANNNNNNTGG 1 cut(s) 633
BstYI RGATCY 2 cut(s) 1239, 1472
BsuRI GGCC 4 cut(s) 638, 733, 1194, 1479
BtsCI GGATG 6 cut(s) 92, 526, 847, 1000, 1424, 1612
BtsIMutI CAGTG 1 cut(s) 1581
BveI ACCTGC 3 cut(s) 787, 1303, 1633
CciI TCATGA 1 cut(s) 414
CfoI GCGC 1 cut(s) 1300
Cfr10I RCCGGY 1 cut(s) 251
Cfr13I GGNCC 4 cut(s) 636, 637, 1359, 1478
Csp6I GTAC 2 cut(s) 551, 980
CspAI ACCGGT 1 cut(s) 251
CviAII CATG 9 cut(s) 257, 355, 369, 415, 465, 629, 643, 1114, 1592
CviQI GTAC 2 cut(s) 551, 980
DdeI CTNAG 5 cut(s) 213, 291, 684, 1347, 1515
DpnI GATC 4 cut(s) 238, 1241, 1459, 1474
DpnII GATC 4 cut(s) 236, 1239, 1457, 1472
DraI TTTAAA 1 cut(s) 705
DraIII CACNNNGTG 2 cut(s) 698, 1317
EaeI YGGCCR 1 cut(s) 731
Eam1104I CTCTTC 2 cut(s) 715, 1578
EarI CTCTTC 2 cut(s) 715, 1578
Eco130I CCWWGG 1 cut(s) 814
Eco24I GRGCYC 1 cut(s) 640
Eco47I GGWCC 1 cut(s) 1359
EcoNI CCTNNNNNAGG 1 cut(s) 818
EcoRI GAATTC 1 cut(s) 1366
EcoRII CCWGG 2 cut(s) 194, 708
EcoT14I CCWWGG 1 cut(s) 814
EcoT38I GRGCYC 1 cut(s) 640
ErhI CCWWGG 1 cut(s) 814
FaeI CATG 9 cut(s) 260, 358, 372, 418, 468, 632, 646, 1117, 1595
FalI AAGNNNNNCTT 2 cut(s) 904, 936
FaqI GGGAC 2 cut(s) 628, 1573
FatI CATG 9 cut(s) 256, 354, 368, 414, 464, 628, 642, 1113, 1591
FauI CCCGC 1 cut(s) 1552
Fnu4HI GCNGC 1 cut(s) 1143
FokI GGATG 6 cut(s) 79, 513, 854, 1007, 1431, 1619
FriOI GRGCYC 1 cut(s) 640
Fsp4HI GCNGC 1 cut(s) 1143
FspBI CTAG 1 cut(s) 110
GlaI GCGC 1 cut(s) 1299
GluI GCNGC 1 cut(s) 1143
HaeIII GGCC 4 cut(s) 638, 733, 1194, 1479
HapII CCGG 2 cut(s) 252, 1362
HhaI GCGC 1 cut(s) 1300
Hin1II CATG 9 cut(s) 260, 358, 372, 418, 468, 632, 646, 1117, 1595
Hin6I GCGC 1 cut(s) 1298
HinP1I GCGC 1 cut(s) 1298
HinfI GANTC 8 cut(s) 10, 44, 273, 830, 945, 1257, 1540, 1586
HpaII CCGG 2 cut(s) 252, 1362
HphI GGTGA 4 cut(s) 884, 1301, 1419, 1428
Hpy166II GTNNAC 2 cut(s) 776, 1292
Hpy188I TCNGA 6 cut(s) 129, 531, 672, 835, 861, 1306
Hpy188III TCNNGA 9 cut(s) 143, 234, 316, 415, 585, 1126, 1172, 1349, 1448
Hpy8I GTNNAC 2 cut(s) 776, 1292
HpyAV CCTTC 5 cut(s) 58, 814, 869, 1406, 1434
HpyCH4III ACNGT 2 cut(s) 605, 1168
HpyCH4IV ACGT 2 cut(s) 84, 1313
HpyF10VI GCNNNNNNNGC 3 cut(s) 365, 689, 773
HpyF3I CTNAG 5 cut(s) 213, 291, 684, 1347, 1515
HpySE526I ACGT 2 cut(s) 84, 1313
Hsp92II CATG 9 cut(s) 260, 358, 372, 418, 468, 632, 646, 1117, 1595
HspAI GCGC 1 cut(s) 1298
Kzo9I GATC 4 cut(s) 236, 1239, 1457, 1472
LguI GCTCTTC 1 cut(s) 715
LmnI GCTCC 4 cut(s) 154, 878, 969, 1123
Lsp1109I GCAGC 1 cut(s) 1129
LweI GCATC 6 cut(s) 101, 136, 197, 1409, 1533, 1637
MaeI CTAG 1 cut(s) 110
MaeII ACGT 2 cut(s) 84, 1313
MaeIII GTNAC 3 cut(s) 231, 713, 1186
MalI GATC 4 cut(s) 238, 1241, 1459, 1474
MbiI CCGCTC 1 cut(s) 972
MboI GATC 4 cut(s) 236, 1239, 1457, 1472
MboII GAAGA 8 cut(s) 53, 587, 732, 924, 1397, 1463, 1595, 1623
MflI RGATCY 2 cut(s) 1239, 1472
MhlI GDGCHC 3 cut(s) 640, 778, 1340
MlyI GAGTC 4 cut(s) 4, 954, 1549, 1595
MroXI GAANNNNTTC 4 cut(s) 134, 536, 916, 1167
MseI TTAA 5 cut(s) 704, 890, 896, 1134, 1149
MslI CAYNNNNRTG 1 cut(s) 631
MspA1I CMGCKG 1 cut(s) 1559
MspI CCGG 2 cut(s) 252, 1362
MspR9I CCNGG 3 cut(s) 196, 710, 1363
MssI GTTTAAAC 1 cut(s) 705
MvaI CCWGG 2 cut(s) 196, 710
MwoI GCNNNNNNNGC 3 cut(s) 365, 689, 773
NciI CCSGG 1 cut(s) 1363
NdeII GATC 4 cut(s) 236, 1239, 1457, 1472
NlaIII CATG 9 cut(s) 260, 358, 372, 418, 468, 632, 646, 1117, 1595
NlaIV GGNNCC 6 cut(s) 137, 170, 539, 638, 1119, 1241
NmuCI GTSAC 1 cut(s) 231
NspV TTCGAA 1 cut(s) 592
PagI TCATGA 1 cut(s) 414
PaqCI CACCTGC 2 cut(s) 787, 1633
PciSI GCTCTTC 1 cut(s) 715
PdmI GAANNNNTTC 4 cut(s) 134, 536, 916, 1167
PfeI GAWTC 4 cut(s) 44, 273, 830, 1257
PflMI CCANNNNNTGG 2 cut(s) 646, 1415
PinAI ACCGGT 1 cut(s) 251
PkrI GCNGC 1 cut(s) 1144
PleI GAGTC 4 cut(s) 4, 953, 1548, 1594
PmeI GTTTAAAC 1 cut(s) 705
PpsI GAGTC 4 cut(s) 4, 953, 1548, 1594
PsiI TTATAA 2 cut(s) 621, 1275
Psp6I CCWGG 2 cut(s) 194, 708
PspGI CCWGG 2 cut(s) 194, 708
PspN4I GGNNCC 6 cut(s) 137, 170, 539, 638, 1119, 1241
PspOMI GGGCCC 1 cut(s) 636
PspPI GGNCC 4 cut(s) 636, 637, 1359, 1478
PsuI RGATCY 2 cut(s) 1239, 1472
RsaI GTAC 2 cut(s) 552, 981
RsaNI GTAC 2 cut(s) 551, 980
RseI CAYNNNNRTG 1 cut(s) 631
SapI GCTCTTC 1 cut(s) 715
SaqAI TTAA 5 cut(s) 704, 890, 896, 1134, 1149
SatI GCNGC 1 cut(s) 1143
Sau3AI GATC 4 cut(s) 236, 1239, 1457, 1472
Sau96I GGNCC 4 cut(s) 636, 637, 1359, 1478
ScaI AGTACT 1 cut(s) 981
SchI GAGTC 4 cut(s) 4, 954, 1549, 1595
ScrFI CCNGG 3 cut(s) 196, 710, 1363
SduI GDGCHC 3 cut(s) 640, 778, 1340
SfaNI GCATC 6 cut(s) 101, 136, 197, 1409, 1533, 1637
SfcI CTRYAG 1 cut(s) 1396
SfuI TTCGAA 1 cut(s) 592
SinI GGWCC 1 cut(s) 1359
SmiMI CAYNNNNRTG 1 cut(s) 631
SmlI CTYRAG 1 cut(s) 1504
SmoI CTYRAG 1 cut(s) 1504
SsiI CCGC 4 cut(s) 205, 535, 972, 1559
SspMI CTAG 1 cut(s) 110
StyD4I CCNGG 3 cut(s) 194, 708, 1361
StyI CCWWGG 1 cut(s) 814
TaaI ACNGT 2 cut(s) 605, 1168
TaiI ACGT 2 cut(s) 87, 1316
TaqI TCGA 4 cut(s) 8, 47, 592, 1155
TatI WGTACW 2 cut(s) 550, 979
TfiI GAWTC 4 cut(s) 44, 273, 830, 1257
Tru1I TTAA 5 cut(s) 704, 890, 896, 1134, 1149
Tru9I TTAA 5 cut(s) 704, 890, 896, 1134, 1149
TscAI CASTG 1 cut(s) 1581
TseFI GTSAC 1 cut(s) 231
TseI GCWGC 1 cut(s) 1142
Tsp45I GTSAC 1 cut(s) 231
TspDTI ATGAA 8 cut(s) 240, 365, 645, 656, 1076, 1176, 1556, 1623
TspGWI ACGGA 1 cut(s) 1110
TspRI CASTG 1 cut(s) 1581
Van91I CCANNNNNTGG 2 cut(s) 646, 1415
VneI GTGCAC 1 cut(s) 774
VpaK11BI GGWCC 1 cut(s) 1359
XagI CCTNNNNNAGG 1 cut(s) 818
XapI RAATTY 3 cut(s) 176, 486, 1366
XmnI GAANNNNTTC 4 cut(s) 134, 536, 916, 1167
XspI CTAG 1 cut(s) 110
ZrmI AGTACT 1 cut(s) 981
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.