Rh1AG374100

Protein of unknown function (DUF2985)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0012162
Physical Location & Seq
Reverse (-)
60708890 .. 60710563
1674 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG374100.1

Sequence Viewer

Length: 1674 bp
ATGGTTTCGACTGATAATGGAAACCAAAAAGAGGAAATTGAAGGCTCTAATGGTGGTGGAGTGAAGGCTCCAGTTCCCTTGGACAACCTTTCTGTGTCTAGTAGGAATTTGTTAAGCGATGAAAAGCCTCAGAGGAGGTTTCATGATGCTATTTCTGATAGGGTAAGGTTCCTGAAATTTGGCTCTGCATCTGCCAGATTTAAGCGGTTAGCTATGGATAGAGATGAGATTTCACGCTCGGTGGCTTCGCCAAGGGGACATGGTTTCAGAGAAAGGATCAGTGGGGTCTTTTCCAAGAAAATTGATTGGCCTTCGCTCATGAAAATGAGCAAAGAGTGGATTAGAGAACCAATGAACATGGCTCTTTTTCTGTGGATTGCCTGTGTTGCTGTTTCAGGTGCAATTTTGTTTCTTGTCATGACTGGAATGTTGAACCATGCACTACCAAAGAAGTCCCAGAGAGATGCATGGTTCGAGGTGAATAATCAAATTCTCAATGCACTTTTTACTCTTATGTGTTTGTACCAGCATCCAAAGCGATTTTACCACCTTGTACTTCTGTGTAGATGGAAACCAGAAGACATTTCTAGACTCAGAAAGATTTACTGTAAAAATGGGACTTATAAGCCACATGAATGGGCACACATGATGGTTGTTGTTGTTCTGCTCCATGTGAATTGCTTTGCACAATATGCGCTTTGTGGTTTAAACTTGGGTTACAAAAGATCTGAACGACCAGCCATAGGAGTTGGAATATGTGTATCTGTTGCAATTGGTGCACCTGCAATTGCTGGTATATACAACATTGTCAGTCCCCTTGGGAAGGATTATGACATGGATGAGGAAGCGCAGCTTCAGTTTACCGCTGATGAAGCAGCTGAAAAGTTGAGGTTAAAGCGATTAGTGAGAAGGTATTCTTTTGCATCCAGGGATGAACATCGAATCATTGAGGCTGCCCCAAAATGGACTGGAGGTATACTGGATATTTGGGATGACATTTCTCTTGCATATGTCTCACTGTTCTGTAGCTTTTGTGTATTTGGGTGGAATATGGACAGACTTGGGTTCGGGAACATGTATGTTCATATTCTGACATTTATTCTGTTTTGCGTGGCTCCTTTCTGGATCTTTAACTTGGCTGCTGTTAATATTGACAATGAGACTGTTCGGGAGTCTCTTGCAGTAACTGGCATTATTCTCTGTGTTTTTGGTTTGCTCTATGGTGGCTTTTGGAGGATCCAAATGAGAAAGAGATTTAACTTGCCTTCTTATAACTTCTGTTTTGGTGAACCGGCAGTGTCTGATTGCGCATTGTGGCTTTTCTGTTGTTGGTGTTCTCTTGCTCAGGAAGTGAGGACGGGGAATTCCTATGACATTGTAGAAGATAAGTTTTGTAAAAAAGAAACAGATATTGGGAGCCAGCTACCAATGTCACCCTTGCCCCGTGAAGATGGAGTGGGTCAACTTCGGTCTGGTCCAAGTTCGCCTTTGGGGAACTACTCAAGCCCAACTAAGACCGGCATGGTTGGTTCTCCAAGTCCTAGCAGATTTTCAAAGGGACTGTATAGTCCAGATAGGGCGCTTTCTTCGGTGAAAGAAGAATCTCATCCAACAGGTAAGGATGAAACTATGACCGCACCTGCACCCTCGGTGATACAAAGAGAAGCCATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2985 PLAC8

Protein Analysis

557

Amino Acids

62.69

Weight (kDa)

8.78

Isoelectric Point (pI)

47.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2985 PF11204 112 - 190 9.6e-32 Protein of unknown function (DUF2985)
PLAC8 PF04749 322 - 452 2.4e-19 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012162)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10980 AT5G05350
fragaria_vesca FvH4_7g25461
malus_domestica MD01G1161000.v1.1 MD07G1229000.v1.1
prunus_persica Prupe.2G259100_v2.0.a1
pyrus_communis pycom01g17680 pycom07g20720
rosa_chinensis RchiOBHm_Chr1g0371431
rosa_laevigata RLG00000026963
rosa_multiflora Rmu_sc0000809.1_g000023 Rmu_sc0017835.1_g000001
rosa_roxburghii Rroxscaffold_4G00285690
rosa_rugosa Rorug01G0363900
rosa_samantha Rh1AG374100 Rh1BG337500 Rh1CG350800 Rh1DG368500
rosa_wichuraiana Rw1G032900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 624, 1272
AarI CACCTGC 2 cut(s) 790, 1648
AasI GACNNNNNNGTC 1 cut(s) 1566
Acc16I TGCGCA 1 cut(s) 1309
Acc36I ACCTGC 2 cut(s) 790, 1648
AccI GTMKAC 1 cut(s) 976
AciI CCGC 3 cut(s) 205, 864, 1635
AclWI GGATC 4 cut(s) 284, 1133, 1231, 1244
AcsI RAATTY 4 cut(s) 106, 176, 489, 1363
AcuI CTGAAG 1 cut(s) 839
AfaI GTAC 2 cut(s) 524, 555
AfiI CCNNNNNNNGG 4 cut(s) 31, 743, 823, 963
AflIII ACRYGT 1 cut(s) 1074
AgsI TTSAA 3 cut(s) 41, 433, 1554
AjnI CCWGG 1 cut(s) 926
AjuI GAANNNNNNNTTGG 2 cut(s) 1395, 1427
AluBI AGCT 5 cut(s) 212, 853, 878, 1029, 1423
AluI AGCT 5 cut(s) 212, 853, 878, 1029, 1423
Alw21I GWGCWC 1 cut(s) 781
Alw26I GTCTC 3 cut(s) 1018, 1154, 1179
Alw44I GTGCAC 1 cut(s) 777
AlwI GGATC 4 cut(s) 284, 1133, 1231, 1244
AlwNI CAGNNNCTG 2 cut(s) 1187, 1301
AoxI GGCC 1 cut(s) 308
ApaLI GTGCAC 1 cut(s) 777
ApeKI GCWGC 4 cut(s) 850, 875, 953, 1139
ApoI RAATTY 4 cut(s) 106, 176, 489, 1363
Asp700I GAANNNNTTC 1 cut(s) 913
AspLEI GCGC 4 cut(s) 697, 850, 1310, 1582
AspS9I GGNCC 1 cut(s) 1475
AsuHPI GGTGA 5 cut(s) 490, 1298, 1425, 1603, 1663
AvaII GGWCC 1 cut(s) 1475
BaeGI GKGCMC 2 cut(s) 643, 781
BamHI GGATCC 1 cut(s) 1236
BbsI GAAGAC 1 cut(s) 585
Bbv12I GWGCWC 1 cut(s) 781
BbvI GCAGC 4 cut(s) 862, 887, 940, 1126
BccI CCATC 3 cut(s) 561, 643, 1445
BciT130I CCWGG 1 cut(s) 928
BcoDI GTCTC 3 cut(s) 1018, 1154, 1179
BfaI CTAG 3 cut(s) 99, 588, 1542
BfmI CTRYAG 1 cut(s) 1024
BfoI RGCGCY 1 cut(s) 1583
BfuAI ACCTGC 2 cut(s) 790, 1648
BglII AGATCT 1 cut(s) 725
BisI GCNGC 4 cut(s) 851, 876, 954, 1140
BlsI GCNGC 4 cut(s) 852, 877, 955, 1141
Bme1390I CCNGG 1 cut(s) 928
Bme18I GGWCC 1 cut(s) 1475
BmgT120I GGNCC 1 cut(s) 1475
BmiI GGNNCC 5 cut(s) 69, 170, 1116, 1238, 1418
BmrFI CCNGG 1 cut(s) 928
BmsI GCATC 5 cut(s) 136, 197, 454, 538, 932
BpiI GAAGAC 1 cut(s) 585
BpmI CTGGAG 2 cut(s) 54, 990
Bpu10I CCTNAGC 1 cut(s) 1344
BpuEI CTTGAG 1 cut(s) 1486
BsaBI GATNNNNATC 1 cut(s) 936
BsaJI CCNNGG 5 cut(s) 78, 251, 817, 927, 1647
Bsc4I CCNNNNNNNGG 4 cut(s) 31, 743, 823, 963
Bse118I RCCGGY 2 cut(s) 1291, 1517
Bse1I ACTGG 5 cut(s) 71, 427, 973, 984, 1192
Bse8I GATNNNNATC 1 cut(s) 936
BseBI CCWGG 1 cut(s) 928
BseDI CCNNGG 5 cut(s) 78, 251, 817, 927, 1647
BseGI GGATG 7 cut(s) 529, 844, 923, 937, 997, 1606, 1627
BseJI GATNNNNATC 1 cut(s) 936
BseLI CCNNNNNNNGG 4 cut(s) 31, 743, 823, 963
BseMII CTCAG 3 cut(s) 143, 607, 1358
BseNI ACTGG 5 cut(s) 71, 427, 973, 984, 1192
BseRI GAGGAG 1 cut(s) 148
BseSI GKGCMC 2 cut(s) 643, 781
BseXI GCAGC 4 cut(s) 862, 887, 940, 1126
BsgI GTGCAG 1 cut(s) 1626
BshFI GGCC 1 cut(s) 310
BsiHKAI GWGCWC 1 cut(s) 781
BsiSI CCGG 2 cut(s) 1292, 1518
BslFI GGGAC 5 cut(s) 270, 439, 631, 798, 1572
BslI CCNNNNNNNGG 4 cut(s) 31, 743, 823, 963
BsmAI GTCTC 3 cut(s) 1018, 1154, 1179
BsmFI GGGAC 5 cut(s) 270, 439, 631, 798, 1572
BsnI GGCC 1 cut(s) 310
Bsp1286I GDGCHC 2 cut(s) 643, 781
Bsp143I GATC 4 cut(s) 276, 725, 1125, 1236
BspACI CCGC 3 cut(s) 205, 864, 1635
BspANI GGCC 1 cut(s) 310
BspCNI CTCAG 3 cut(s) 142, 606, 1357
BspHI TCATGA 3 cut(s) 142, 318, 417
BspLI GGNNCC 5 cut(s) 69, 170, 1116, 1238, 1418
BspMI ACCTGC 2 cut(s) 790, 1648
BspPI GGATC 4 cut(s) 284, 1133, 1231, 1244
BsrFI RCCGGY 2 cut(s) 1291, 1517
BsrI ACTGG 5 cut(s) 71, 427, 973, 984, 1192
BssAI RCCGGY 2 cut(s) 1291, 1517
BssECI CCNNGG 5 cut(s) 78, 251, 817, 927, 1647
BssMI GATC 4 cut(s) 276, 725, 1125, 1236
BssNAI GTATAC 1 cut(s) 977
BssT1I CCWWGG 3 cut(s) 78, 251, 817
Bst1107I GTATAC 1 cut(s) 977
Bst2UI CCWGG 1 cut(s) 928
Bst4CI ACNGT 4 cut(s) 608, 1020, 1165, 1563
BstAPI GCANNNNNTGC 2 cut(s) 692, 776
BstC8I GCNNGC 1 cut(s) 1421
BstDEI CTNAG 4 cut(s) 129, 593, 1344, 1512
BstENI CCTNNNNNAGG 1 cut(s) 821
BstF5I GGATG 7 cut(s) 529, 844, 923, 937, 997, 1606, 1627
BstH2I RGCGCY 1 cut(s) 1583
BstHHI GCGC 4 cut(s) 697, 850, 1310, 1582
BstKTI GATC 4 cut(s) 279, 728, 1128, 1239
BstMAI GTCTC 3 cut(s) 1018, 1154, 1179
BstMBI GATC 4 cut(s) 276, 725, 1125, 1236
BstMWI GCNNNNNNNGC 5 cut(s) 386, 535, 692, 776, 872
BstNI CCWGG 1 cut(s) 928
BstNSI RCATGY 1 cut(s) 1078
BstSCI CCNGG 1 cut(s) 926
BstSFI CTRYAG 1 cut(s) 1024
BstSLI GKGCMC 2 cut(s) 643, 781
BstV1I GCAGC 4 cut(s) 862, 887, 940, 1126
BstV2I GAAGAC 1 cut(s) 585
BstX2I RGATCY 3 cut(s) 725, 1125, 1236
BstXI CCANNNNNNTGG 1 cut(s) 636
BstYI RGATCY 3 cut(s) 725, 1125, 1236
BstZ17I GTATAC 1 cut(s) 977
BsuRI GGCC 1 cut(s) 310
BtgZI GCGATG 1 cut(s) 132
BtsCI GGATG 7 cut(s) 529, 844, 923, 937, 997, 1606, 1627
BtsI GCAGTG 1 cut(s) 1302
BtsIMutI CAGTG 3 cut(s) 286, 1016, 1302
BveI ACCTGC 2 cut(s) 790, 1648
Cac8I GCNNGC 1 cut(s) 1421
CaiI CAGNNNCTG 2 cut(s) 1187, 1301
CciI TCATGA 3 cut(s) 142, 318, 417
CfoI GCGC 4 cut(s) 697, 850, 1310, 1582
Cfr10I RCCGGY 2 cut(s) 1291, 1517
Cfr13I GGNCC 1 cut(s) 1475
Csp6I GTAC 2 cut(s) 523, 554
CviQI GTAC 2 cut(s) 523, 554
DdeI CTNAG 4 cut(s) 129, 593, 1344, 1512
DpnI GATC 4 cut(s) 278, 727, 1127, 1238
DpnII GATC 4 cut(s) 276, 725, 1125, 1236
DraI TTTAAA 1 cut(s) 708
DrdI GACNNNNNNGTC 1 cut(s) 1566
DseDI GACNNNNNNGTC 1 cut(s) 1566
Eco130I CCWWGG 3 cut(s) 78, 251, 817
Eco47I GGWCC 1 cut(s) 1475
Eco57I CTGAAG 1 cut(s) 839
EcoNI CCTNNNNNAGG 1 cut(s) 821
EcoRI GAATTC 1 cut(s) 1363
EcoRII CCWGG 1 cut(s) 926
EcoT14I CCWWGG 3 cut(s) 78, 251, 817
EcoT22I ATGCAT 1 cut(s) 469
ErhI CCWWGG 3 cut(s) 78, 251, 817
FalI AAGNNNNNCTT 6 cut(s) 837, 869, 901, 933, 1471, 1503
FaqI GGGAC 5 cut(s) 270, 439, 631, 798, 1572
FauNDI CATATG 1 cut(s) 1009
FblI GTMKAC 1 cut(s) 976
Fnu4HI GCNGC 4 cut(s) 851, 876, 954, 1140
FokI GGATG 7 cut(s) 516, 851, 910, 944, 1004, 1593, 1634
Fsp4HI GCNGC 4 cut(s) 851, 876, 954, 1140
FspBI CTAG 3 cut(s) 99, 588, 1542
FspI TGCGCA 1 cut(s) 1309
GlaI GCGC 4 cut(s) 696, 849, 1309, 1581
GluI GCNGC 4 cut(s) 851, 876, 954, 1140
GsuI CTGGAG 2 cut(s) 54, 990
HaeII RGCGCY 1 cut(s) 1583
HaeIII GGCC 1 cut(s) 310
HapII CCGG 2 cut(s) 1292, 1518
HhaI GCGC 4 cut(s) 697, 850, 1310, 1582
Hin6I GCGC 4 cut(s) 695, 848, 1308, 1580
HinP1I GCGC 4 cut(s) 695, 848, 1308, 1580
HincII GTYRAC 1 cut(s) 1463
HindII GTYRAC 1 cut(s) 1463
HinfI GANTC 4 cut(s) 591, 942, 1172, 1601
HpaII CCGG 2 cut(s) 1292, 1518
HphI GGTGA 5 cut(s) 490, 1298, 1425, 1603, 1663
Hpy166II GTNNAC 5 cut(s) 779, 861, 977, 1289, 1463
Hpy188I TCNGA 7 cut(s) 132, 157, 269, 596, 730, 1092, 1303
Hpy8I GTNNAC 5 cut(s) 779, 861, 977, 1289, 1463
HpyAV CCTTC 6 cut(s) 35, 58, 321, 817, 903, 1275
HpyCH4III ACNGT 4 cut(s) 608, 1020, 1165, 1563
HpyF10VI GCNNNNNNNGC 5 cut(s) 386, 535, 692, 776, 872
HpyF3I CTNAG 4 cut(s) 129, 593, 1344, 1512
HspAI GCGC 4 cut(s) 695, 848, 1308, 1580
Kzo9I GATC 4 cut(s) 276, 725, 1125, 1236
LmnI GCTCC 4 cut(s) 73, 672, 1120, 1416
Lsp1109I GCAGC 4 cut(s) 862, 887, 940, 1126
LweI GCATC 5 cut(s) 136, 197, 454, 538, 932
MaeI CTAG 3 cut(s) 99, 588, 1542
MaeIII GTNAC 3 cut(s) 716, 1183, 1431
MalI GATC 4 cut(s) 278, 727, 1127, 1238
MboI GATC 4 cut(s) 276, 725, 1125, 1236
MboII GAAGA 5 cut(s) 590, 1394, 1460, 1578, 1610
MfeI CAATTG 2 cut(s) 771, 786
MflI RGATCY 3 cut(s) 725, 1125, 1236
MhlI GDGCHC 2 cut(s) 643, 781
MlyI GAGTC 2 cut(s) 585, 1181
MmeI TCCRAC 2 cut(s) 730, 1634
Mph1103I ATGCAT 1 cut(s) 469
MroXI GAANNNNTTC 1 cut(s) 913
MseI TTAA 7 cut(s) 113, 201, 707, 893, 1131, 1146, 1257
MslI CAYNNNNRTG 2 cut(s) 323, 634
MspA1I CMGCKG 2 cut(s) 866, 878
MspI CCGG 2 cut(s) 1292, 1518
MspR9I CCNGG 1 cut(s) 928
MssI GTTTAAAC 1 cut(s) 708
MunI CAATTG 2 cut(s) 771, 786
MvaI CCWGG 1 cut(s) 928
MwoI GCNNNNNNNGC 5 cut(s) 386, 535, 692, 776, 872
NdeI CATATG 1 cut(s) 1009
NdeII GATC 4 cut(s) 276, 725, 1125, 1236
NlaIV GGNNCC 5 cut(s) 69, 170, 1116, 1238, 1418
NmuCI GTSAC 1 cut(s) 1431
NsbI TGCGCA 1 cut(s) 1309
NsiI ATGCAT 1 cut(s) 469
NspI RCATGY 1 cut(s) 1078
PagI TCATGA 3 cut(s) 142, 318, 417
PaqCI CACCTGC 2 cut(s) 790, 1648
PciI ACATGT 1 cut(s) 1074
PdmI GAANNNNTTC 1 cut(s) 913
PfeI GAWTC 2 cut(s) 942, 1601
PkrI GCNGC 4 cut(s) 852, 877, 955, 1141
PleI GAGTC 2 cut(s) 585, 1180
PmeI GTTTAAAC 1 cut(s) 708
PpsI GAGTC 2 cut(s) 585, 1180
PscI ACATGT 1 cut(s) 1074
PsiI TTATAA 2 cut(s) 624, 1272
Psp6I CCWGG 1 cut(s) 926
PspGI CCWGG 1 cut(s) 926
PspN4I GGNNCC 5 cut(s) 69, 170, 1116, 1238, 1418
PspPI GGNCC 1 cut(s) 1475
PstNI CAGNNNCTG 2 cut(s) 1187, 1301
PsuI RGATCY 3 cut(s) 725, 1125, 1236
PvuII CAGCTG 1 cut(s) 878
RsaI GTAC 2 cut(s) 524, 555
RsaNI GTAC 2 cut(s) 523, 554
RseI CAYNNNNRTG 2 cut(s) 323, 634
SaqAI TTAA 7 cut(s) 113, 201, 707, 893, 1131, 1146, 1257
SatI GCNGC 4 cut(s) 851, 876, 954, 1140
Sau3AI GATC 4 cut(s) 276, 725, 1125, 1236
Sau96I GGNCC 1 cut(s) 1475
SchI GAGTC 2 cut(s) 585, 1181
ScrFI CCNGG 1 cut(s) 928
SduI GDGCHC 2 cut(s) 643, 781
SfaNI GCATC 5 cut(s) 136, 197, 454, 538, 932
SfcI CTRYAG 1 cut(s) 1024
SinI GGWCC 1 cut(s) 1475
SmiMI CAYNNNNRTG 2 cut(s) 323, 634
SmlI CTYRAG 1 cut(s) 1501
SmoI CTYRAG 1 cut(s) 1501
SsiI CCGC 3 cut(s) 205, 864, 1635
SspI AATATT 1 cut(s) 1150
SspMI CTAG 3 cut(s) 99, 588, 1542
StyD4I CCNGG 1 cut(s) 926
StyI CCWWGG 3 cut(s) 78, 251, 817
TaaI ACNGT 4 cut(s) 608, 1020, 1165, 1563
TaqI TCGA 3 cut(s) 8, 474, 940
TaqII GACCGA 1 cut(s) 1458
TatI WGTACW 1 cut(s) 553
TfiI GAWTC 2 cut(s) 942, 1601
Tru1I TTAA 7 cut(s) 113, 201, 707, 893, 1131, 1146, 1257
Tru9I TTAA 7 cut(s) 113, 201, 707, 893, 1131, 1146, 1257
TscAI CASTG 3 cut(s) 286, 1023, 1302
TseFI GTSAC 1 cut(s) 1431
TseI GCWGC 4 cut(s) 850, 875, 953, 1139
Tsp45I GTSAC 1 cut(s) 1431
TspDTI ATGAA 9 cut(s) 131, 135, 335, 368, 648, 885, 948, 1073, 1638
TspRI CASTG 3 cut(s) 286, 1023, 1302
VneI GTGCAC 1 cut(s) 777
VpaK11BI GGWCC 1 cut(s) 1475
XagI CCTNNNNNAGG 1 cut(s) 821
XapI RAATTY 4 cut(s) 106, 176, 489, 1363
XbaI TCTAGA 1 cut(s) 587
XceI RCATGY 1 cut(s) 1078
XmiI GTMKAC 1 cut(s) 976
XmnI GAANNNNTTC 1 cut(s) 913
XspI CTAG 3 cut(s) 99, 588, 1542
Zsp2I ATGCAT 1 cut(s) 469
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.