MD07G1229000.v1.1

Protein of unknown function (DUF2985)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0012162
Physical Location & Seq
Reverse (-)
30415764 .. 30418363
2600 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1229000.v1.1.491

Sequence Viewer

Length: 1668 bp
ATGGTTTCGACTCAAAATGGAAACCACAAGGAGGAAATTGAAGAATCGAATGGTAATGAAGTGAAGGGTCAAATCCCCTTTGACGTTTCAGCATCCCCAAAGACTTTTTTAGTCAATGAGAAGTCTCCGAAAAGGTTCCCTCCTGATGCTCCTAATCTAGTTAGGTTCCTCAAATTTGGTTCTGCATCTGCCAGGTTCAAGCGGTTGGCTGAGGAGAGAGATGAAATGTCACGATCTGTGGCTTCAAGCGGTCATGGCTTCAAAGAACGAATCAATGGGGTTTTTTCTAAGAAAATTGATTGGGTTTCACTCCTGAAAATGGGCAGAGTATGGATTAGAAATCCAATGAACATGGCTCTTTTTGCATGGATTGTCTGTGTTGCTATTTCGGGTGCAATTTTATTTCTTGTCATGACTGGAATGTTGAACAAGGCAATACCCAAGAAGTCCCAGAGAGATACATGGTTCGAAGTCAATAATCAAATTCTCAATGCACTTTTTACGCTGATGTGTTTGTACAACCATCCGAAGCGGTTCCACCACCTTGTACTTCTGTGTAGATGGAAACCAGAAGACATTTCCAGACTTCGAAAGATTTACTGTAAAAATGGGACTTATAAGCCACACGAATGGGCCCACATGATGGTTGTTGTTGCCCTGCTTCATCTGAATTGCTTTGCTCAGTATGCACTTTGTGGTTTAAACCTGGGTTACAGAAGAGTTGAACGGCCACCCATAGGAGTTGCAGTATGTGTATCTTTTGCTATTGGTGCACCTGCATTTGCTGGTATATACAACATTGTCAGCCCCCTTGGGAAGGATTATGATTCAGATGTGGATGAGGAAGCACAGGTTCAGATCGGAACTGGTGAAGGAGCAGAAAGATTAAGGTTAAAATCATTCGAGAGAAGATATTCTTTTGCATTTAGGACTGAACAACGAGTCGTTGAGAGTAGACCACAATGGAGCGGAGGAGTACTTGACATTTGGGATGATATTTCTATAGCGTATGTTTCACTCTTTTGTAGTTTTTGTGTATTCGGGTGGAATATGGAGAGGCTTGGATTCGGCAACGTATATGTTCATATTATGACATTTATTCTGTTTTGCATGGCTCCGTTCTGGATTTTTAACTTGGCTGCTGTTAATATCGACAATGAAACTGTTCGGGAGGCTCTTGGAGTTACTGGCCTTGTTCTATGTCTATTTGGCTTGCTCTATGGTGGCTTTTGGAGGATCCAAATGAGAAAGAGATTCAATTTGCCTTCTTATAACTTCTGTTTTGGTGAACCAGCACTCTCTGATTGCACGTTGTGGCTTTGCTGTTGTTGGTGCTCTCTTGCTCAGGAAGTGAGGACCGGGAATTCCTATGACATTGTGGAAGAAAAGTTCTATAGAAAACAACCGGAAAGTGGAAGCCCTTTACCAATTTCACCTTTGCCTCGTGAAGATGGATTGGTTGGGTTCAGATGTGGCCCGAATTCTCCTCTGGGGAACTACTCAAGCCCAACCAAGAATGGTGTCGGTGGTTCTCCAAGTCCAAGCAGACTTTCAATGGGATTTCATAGTCCAGTGAAAGAAGAGTCCCATGTAAGAGGTAAGGATGAAACTATGACCCCACCTGCACCTTCAATGATACAAAGGGAAGAAACACAGCCCCAAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2985 PLAC8

Protein Analysis

556

Amino Acids

62.77

Weight (kDa)

8.83

Isoelectric Point (pI)

47.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2985 PF11204 111 - 188 2.9e-30 Protein of unknown function (DUF2985)
PLAC8 PF04749 322 - 452 1.8e-19 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012162)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10980 AT5G05350
fragaria_vesca FvH4_7g25461
malus_domestica MD01G1161000.v1.1 MD07G1229000.v1.1
prunus_persica Prupe.2G259100_v2.0.a1
pyrus_communis pycom01g17680 pycom07g20720
rosa_chinensis RchiOBHm_Chr1g0371431
rosa_laevigata RLG00000026963
rosa_multiflora Rmu_sc0000809.1_g000023 Rmu_sc0017835.1_g000001
rosa_roxburghii Rroxscaffold_4G00285690
rosa_rugosa Rorug01G0363900
rosa_samantha Rh1AG374100 Rh1BG337500 Rh1CG350800 Rh1DG368500
rosa_wichuraiana Rw1G032900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 618, 1272
AarI CACCTGC 2 cut(s) 784, 1630
Acc36I ACCTGC 2 cut(s) 784, 1630
AccB7I CCANNNNNTGG 1 cut(s) 643
AccBSI CCGCTC 1 cut(s) 969
AccI GTMKAC 1 cut(s) 955
AciI CCGC 4 cut(s) 202, 249, 532, 969
AclWI GGATC 2 cut(s) 1231, 1244
AcoI YGGCCR 1 cut(s) 728
AcsI RAATTY 4 cut(s) 173, 483, 1363, 1480
AdeI CACNNNGTG 2 cut(s) 695, 1314
AfaI GTAC 3 cut(s) 518, 549, 978
AfiI CCNNNNNNNGG 6 cut(s) 31, 319, 643, 737, 817, 1412
AgsI TTSAA 9 cut(s) 41, 199, 246, 262, 427, 725, 1258, 1554, 1632
AjnI CCWGG 2 cut(s) 191, 705
AjuI GAANNNNNNNTTGG 2 cut(s) 1534, 1566
AloI GAACNNNNNNTCC 2 cut(s) 1373, 1405
Alw21I GWGCWC 2 cut(s) 775, 1337
Alw26I GTCTC 1 cut(s) 129
Alw44I GTGCAC 1 cut(s) 771
AlwI GGATC 2 cut(s) 1231, 1244
AoxI GGCC 4 cut(s) 633, 728, 1189, 1474
ApaI GGGCCC 1 cut(s) 637
ApaLI GTGCAC 1 cut(s) 771
ApeKI GCWGC 1 cut(s) 1139
ApoI RAATTY 4 cut(s) 173, 483, 1363, 1480
Asp700I GAANNNNTTC 4 cut(s) 134, 533, 913, 1164
AspS9I GGNCC 4 cut(s) 633, 634, 1356, 1475
AsuC2I CCSGG 1 cut(s) 1360
AsuHPI GGTGA 3 cut(s) 881, 1298, 1425
AsuII TTCGAA 2 cut(s) 468, 589
AvaII GGWCC 1 cut(s) 1356
BaeGI GKGCMC 2 cut(s) 637, 775
BamHI GGATCC 1 cut(s) 1236
BanII GRGCYC 1 cut(s) 637
BauI CACGAG 1 cut(s) 1443
BbsI GAAGAC 1 cut(s) 579
Bbv12I GWGCWC 2 cut(s) 775, 1337
BbvCI CCTCAGC 1 cut(s) 210
BbvI GCAGC 1 cut(s) 1126
BccI CCATC 4 cut(s) 531, 555, 637, 1445
BceAI ACGGC 1 cut(s) 743
BciT130I CCWGG 2 cut(s) 193, 707
BcnI CCSGG 1 cut(s) 1360
BcoDI GTCTC 1 cut(s) 129
BfaI CTAG 1 cut(s) 158
BfmI CTRYAG 2 cut(s) 1002, 1393
BfuAI ACCTGC 2 cut(s) 784, 1630
BisI GCNGC 1 cut(s) 1140
BlsI GCNGC 1 cut(s) 1141
BmcAI AGTACT 1 cut(s) 978
Bme1390I CCNGG 3 cut(s) 193, 707, 1360
Bme18I GGWCC 1 cut(s) 1356
BmgT120I GGNCC 4 cut(s) 633, 634, 1356, 1475
BmiI GGNNCC 6 cut(s) 137, 167, 536, 635, 1116, 1238
BmrFI CCNGG 3 cut(s) 193, 707, 1360
BmsI GCATC 3 cut(s) 101, 136, 194
BpiI GAAGAC 1 cut(s) 579
Bpu10I CCTNAGC 2 cut(s) 210, 1344
Bpu14I TTCGAA 2 cut(s) 468, 589
BpuEI CTTGAG 1 cut(s) 1486
BpuMI CCSGG 1 cut(s) 1360
BsaJI CCNNGG 2 cut(s) 706, 811
BsaWI WCCGGW 1 cut(s) 1405
BsaXI ACNNNNNCTCC 2 cut(s) 732, 762
Bsc4I CCNNNNNNNGG 6 cut(s) 31, 319, 643, 737, 817, 1412
Bse1I ACTGG 4 cut(s) 421, 871, 1192, 1571
BseBI CCWGG 2 cut(s) 193, 707
BseDI CCNNGG 2 cut(s) 706, 811
BseGI GGATG 5 cut(s) 92, 523, 844, 997, 1609
BseLI CCNNNNNNNGG 6 cut(s) 31, 319, 643, 737, 817, 1412
BseMII CTCAG 3 cut(s) 201, 695, 1358
BseNI ACTGG 4 cut(s) 421, 871, 1192, 1571
BseRI GAGGAG 3 cut(s) 227, 987, 1476
BseSI GKGCMC 2 cut(s) 637, 775
BseXI GCAGC 1 cut(s) 1126
BsgI GTGCAG 1 cut(s) 1608
BshFI GGCC 4 cut(s) 635, 730, 1191, 1476
BsiHKAI GWGCWC 2 cut(s) 775, 1337
BsiSI CCGG 2 cut(s) 1359, 1406
BslFI GGGAC 3 cut(s) 433, 625, 1570
BslI CCNNNNNNNGG 6 cut(s) 31, 319, 643, 737, 817, 1412
BsmAI GTCTC 1 cut(s) 129
BsmFI GGGAC 3 cut(s) 433, 625, 1570
BsnI GGCC 4 cut(s) 635, 730, 1191, 1476
Bsp119I TTCGAA 2 cut(s) 468, 589
Bsp120I GGGCCC 1 cut(s) 633
Bsp1286I GDGCHC 3 cut(s) 637, 775, 1337
Bsp1407I TGTACA 1 cut(s) 516
Bsp143I GATC 3 cut(s) 233, 858, 1236
BspACI CCGC 4 cut(s) 202, 249, 532, 969
BspANI GGCC 4 cut(s) 635, 730, 1191, 1476
BspCNI CTCAG 3 cut(s) 202, 694, 1357
BspHI TCATGA 1 cut(s) 411
BspLI GGNNCC 6 cut(s) 137, 167, 536, 635, 1116, 1238
BspMI ACCTGC 2 cut(s) 784, 1630
BspPI GGATC 2 cut(s) 1231, 1244
BspT104I TTCGAA 2 cut(s) 468, 589
BsrBI CCGCTC 1 cut(s) 969
BsrGI TGTACA 1 cut(s) 516
BsrI ACTGG 4 cut(s) 421, 871, 1192, 1571
BssECI CCNNGG 2 cut(s) 706, 811
BssMI GATC 3 cut(s) 233, 858, 1236
BssSI CACGAG 1 cut(s) 1443
BssT1I CCWWGG 1 cut(s) 811
Bst2BI CACGAG 1 cut(s) 1443
Bst2UI CCWGG 2 cut(s) 193, 707
Bst4CI ACNGT 2 cut(s) 602, 1165
Bst6I CTCTTC 2 cut(s) 712, 1575
BstAUI TGTACA 1 cut(s) 516
BstBI TTCGAA 2 cut(s) 468, 589
BstC8I GCNNGC 1 cut(s) 1214
BstDEI CTNAG 4 cut(s) 210, 288, 681, 1344
BstENI CCTNNNNNAGG 1 cut(s) 815
BstF5I GGATG 5 cut(s) 92, 523, 844, 997, 1609
BstKTI GATC 3 cut(s) 236, 861, 1239
BstMAI GTCTC 1 cut(s) 129
BstMBI GATC 3 cut(s) 233, 858, 1236
BstMWI GCNNNNNNNGC 4 cut(s) 255, 362, 686, 770
BstNI CCWGG 2 cut(s) 193, 707
BstSCI CCNGG 3 cut(s) 191, 705, 1358
BstSFI CTRYAG 2 cut(s) 1002, 1393
BstSLI GKGCMC 2 cut(s) 637, 775
BstV1I GCAGC 1 cut(s) 1126
BstV2I GAAGAC 1 cut(s) 579
BstX2I RGATCY 1 cut(s) 1236
BstXI CCANNNNNNTGG 1 cut(s) 630
BstYI RGATCY 1 cut(s) 1236
BsuRI GGCC 4 cut(s) 635, 730, 1191, 1476
BtsCI GGATG 5 cut(s) 92, 523, 844, 997, 1609
BtsIMutI CAGTG 1 cut(s) 1578
BveI ACCTGC 2 cut(s) 784, 1630
Cac8I GCNNGC 1 cut(s) 1214
CciI TCATGA 1 cut(s) 411
Cfr13I GGNCC 4 cut(s) 633, 634, 1356, 1475
Csp6I GTAC 3 cut(s) 517, 548, 977
CviAII CATG 8 cut(s) 254, 352, 366, 412, 462, 640, 1111, 1589
CviQI GTAC 3 cut(s) 517, 548, 977
DdeI CTNAG 4 cut(s) 210, 288, 681, 1344
DpnI GATC 3 cut(s) 235, 860, 1238
DpnII GATC 3 cut(s) 233, 858, 1236
DraI TTTAAA 1 cut(s) 702
DraIII CACNNNGTG 2 cut(s) 695, 1314
EaeI YGGCCR 1 cut(s) 728
Eam1104I CTCTTC 2 cut(s) 712, 1575
EarI CTCTTC 2 cut(s) 712, 1575
Eco130I CCWWGG 1 cut(s) 811
Eco24I GRGCYC 1 cut(s) 637
Eco47I GGWCC 1 cut(s) 1356
EcoNI CCTNNNNNAGG 1 cut(s) 815
EcoRI GAATTC 2 cut(s) 1363, 1480
EcoRII CCWGG 2 cut(s) 191, 705
EcoT14I CCWWGG 1 cut(s) 811
EcoT38I GRGCYC 1 cut(s) 637
ErhI CCWWGG 1 cut(s) 811
FaeI CATG 8 cut(s) 257, 355, 369, 415, 465, 643, 1114, 1592
FalI AAGNNNNNCTT 2 cut(s) 901, 933
FaqI GGGAC 3 cut(s) 433, 625, 1570
FatI CATG 8 cut(s) 253, 351, 365, 411, 461, 639, 1110, 1588
FblI GTMKAC 1 cut(s) 955
Fnu4HI GCNGC 1 cut(s) 1140
FokI GGATG 5 cut(s) 79, 510, 851, 1004, 1616
FriOI GRGCYC 1 cut(s) 637
Fsp4HI GCNGC 1 cut(s) 1140
FspBI CTAG 1 cut(s) 158
GluI GCNGC 1 cut(s) 1140
HaeIII GGCC 4 cut(s) 635, 730, 1191, 1476
HapII CCGG 2 cut(s) 1359, 1406
Hin1II CATG 8 cut(s) 257, 355, 369, 415, 465, 643, 1114, 1592
HinfI GANTC 8 cut(s) 10, 44, 270, 827, 942, 1065, 1254, 1583
HpaII CCGG 2 cut(s) 1359, 1406
HphI GGTGA 3 cut(s) 881, 1298, 1425
Hpy166II GTNNAC 3 cut(s) 773, 956, 1289
Hpy188I TCNGA 8 cut(s) 129, 528, 669, 832, 858, 863, 1303, 1469
Hpy8I GTNNAC 3 cut(s) 773, 956, 1289
HpyAV CCTTC 5 cut(s) 58, 811, 866, 1275, 1638
HpyCH4III ACNGT 2 cut(s) 602, 1165
HpyCH4IV ACGT 3 cut(s) 84, 1074, 1310
HpyF10VI GCNNNNNNNGC 4 cut(s) 255, 362, 686, 770
HpyF3I CTNAG 4 cut(s) 210, 288, 681, 1344
HpySE526I ACGT 3 cut(s) 84, 1074, 1310
Hsp92II CATG 8 cut(s) 257, 355, 369, 415, 465, 643, 1114, 1592
Kzo9I GATC 3 cut(s) 233, 858, 1236
LmnI GCTCC 4 cut(s) 154, 875, 966, 1120
Lsp1109I GCAGC 1 cut(s) 1126
LweI GCATC 3 cut(s) 101, 136, 194
MaeI CTAG 1 cut(s) 158
MaeII ACGT 3 cut(s) 84, 1074, 1310
MaeIII GTNAC 3 cut(s) 228, 710, 1183
MalI GATC 3 cut(s) 235, 860, 1238
MbiI CCGCTC 1 cut(s) 969
MboI GATC 3 cut(s) 233, 858, 1236
MboII GAAGA 8 cut(s) 53, 584, 729, 921, 1394, 1460, 1592, 1658
MflI RGATCY 1 cut(s) 1236
MhlI GDGCHC 3 cut(s) 637, 775, 1337
MlyI GAGTC 3 cut(s) 4, 951, 1592
MroXI GAANNNNTTC 4 cut(s) 134, 533, 913, 1164
MseI TTAA 5 cut(s) 701, 887, 893, 1131, 1146
MslI CAYNNNNRTG 1 cut(s) 628
MspI CCGG 2 cut(s) 1359, 1406
MspR9I CCNGG 3 cut(s) 193, 707, 1360
MssI GTTTAAAC 1 cut(s) 702
MvaI CCWGG 2 cut(s) 193, 707
MwoI GCNNNNNNNGC 4 cut(s) 255, 362, 686, 770
NciI CCSGG 1 cut(s) 1360
NdeII GATC 3 cut(s) 233, 858, 1236
NlaIII CATG 8 cut(s) 257, 355, 369, 415, 465, 643, 1114, 1592
NlaIV GGNNCC 6 cut(s) 137, 167, 536, 635, 1116, 1238
NmuCI GTSAC 1 cut(s) 228
NspV TTCGAA 2 cut(s) 468, 589
PagI TCATGA 1 cut(s) 411
PaqCI CACCTGC 2 cut(s) 784, 1630
PdmI GAANNNNTTC 4 cut(s) 134, 533, 913, 1164
PfeI GAWTC 5 cut(s) 44, 270, 827, 1065, 1254
PflMI CCANNNNNTGG 1 cut(s) 643
PkrI GCNGC 1 cut(s) 1141
PleI GAGTC 3 cut(s) 4, 950, 1591
PmeI GTTTAAAC 1 cut(s) 702
PpsI GAGTC 3 cut(s) 4, 950, 1591
PsiI TTATAA 2 cut(s) 618, 1272
Psp6I CCWGG 2 cut(s) 191, 705
PspGI CCWGG 2 cut(s) 191, 705
PspN4I GGNNCC 6 cut(s) 137, 167, 536, 635, 1116, 1238
PspOMI GGGCCC 1 cut(s) 633
PspPI GGNCC 4 cut(s) 633, 634, 1356, 1475
PsuI RGATCY 1 cut(s) 1236
RsaI GTAC 3 cut(s) 518, 549, 978
RsaNI GTAC 3 cut(s) 517, 548, 977
RseI CAYNNNNRTG 1 cut(s) 628
SaqAI TTAA 5 cut(s) 701, 887, 893, 1131, 1146
SatI GCNGC 1 cut(s) 1140
Sau3AI GATC 3 cut(s) 233, 858, 1236
Sau96I GGNCC 4 cut(s) 633, 634, 1356, 1475
ScaI AGTACT 1 cut(s) 978
SchI GAGTC 3 cut(s) 4, 951, 1592
ScrFI CCNGG 3 cut(s) 193, 707, 1360
SduI GDGCHC 3 cut(s) 637, 775, 1337
SfaNI GCATC 3 cut(s) 101, 136, 194
SfcI CTRYAG 2 cut(s) 1002, 1393
SfuI TTCGAA 2 cut(s) 468, 589
SinI GGWCC 1 cut(s) 1356
SmiMI CAYNNNNRTG 1 cut(s) 628
SmlI CTYRAG 1 cut(s) 1501
SmoI CTYRAG 1 cut(s) 1501
SsiI CCGC 4 cut(s) 202, 249, 532, 969
SspMI CTAG 1 cut(s) 158
StyD4I CCNGG 3 cut(s) 191, 705, 1358
StyI CCWWGG 1 cut(s) 811
TaaI ACNGT 2 cut(s) 602, 1165
TaiI ACGT 3 cut(s) 87, 1077, 1313
TaqI TCGA 6 cut(s) 8, 47, 468, 589, 903, 1152
TatI WGTACW 3 cut(s) 516, 547, 976
TfiI GAWTC 5 cut(s) 44, 270, 827, 1065, 1254
Tru1I TTAA 5 cut(s) 701, 887, 893, 1131, 1146
Tru9I TTAA 5 cut(s) 701, 887, 893, 1131, 1146
TscAI CASTG 1 cut(s) 1578
TseFI GTSAC 1 cut(s) 228
TseI GCWGC 1 cut(s) 1139
Tsp45I GTSAC 1 cut(s) 228
TspDTI ATGAA 8 cut(s) 72, 237, 362, 653, 1073, 1173, 1553, 1620
TspGWI ACGGA 1 cut(s) 1107
TspRI CASTG 1 cut(s) 1578
Van91I CCANNNNNTGG 1 cut(s) 643
VneI GTGCAC 1 cut(s) 771
VpaK11BI GGWCC 1 cut(s) 1356
XagI CCTNNNNNAGG 1 cut(s) 815
XapI RAATTY 4 cut(s) 173, 483, 1363, 1480
XmiI GTMKAC 1 cut(s) 955
XmnI GAANNNNTTC 4 cut(s) 134, 533, 913, 1164
XspI CTAG 1 cut(s) 158
ZrmI AGTACT 1 cut(s) 978
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.