pycom01g17680

Protein of unknown function (DUF2985)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0012162
Physical Location & Seq
Reverse (-)
16869877 .. 16871199
1323 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g17680.1

Sequence Viewer

Length: 1323 bp
ATGGGCAAAAAATGGATTAGAAACCCAATGAACATGGCTCTTTTTGCGTGGATCGTCTGTGTTGCTGTTTCAGGTGCAATTTTGTTCCTTGTGATGACTGGAATGTTGAACAAGGCAATACCCAAGAAGTCCCAGAGAGATGCATGGTTTGAAGTCAATAATCAAATTCTCAACGCACTTTTTACTCTTTTGTGTTTGTACAACCATCCGAAGCGATTCTACCACCTTGTACTTCTGTGTAGATGGAAACCAGAAGACATTTCCAGACTTCGAAAGGTTTACTGTAAAAATGGGACTTATAAACCCCACGAATGGGCCCACATGATGGTTGTTGTTGCCCTGCTTCATCTGAATTGCTTTGCTCAGTATGCACTTTGTAGTTTAAACCTGGGTTACAGAAGAGCTCAACGACCACCGATAGGAGTTGCAATATGTGTATCTTTTGCTATTGGTGCACCTGCATTTGCTGGCATATACAACATTGTCAGCCCCCTCGGGAAGGATTATGATTTAGAAGTGGATGAGGAAGCACAGGTTCCAATCGGCACTGGTGAAGGAGCAGAAAAATTAAGGTCAAAAACATTTGAGAGAAAATATTCTTTTGCATTTAGGACTGAACAACGAGTCGTTGAGAGTAAACCACAATGGAGCGGAGGAGTACTTGACATTTGGGATGATATTTCTATAGCGTATGTTTCACTCTTTTGTAGTTTTTGTGTGTTCGGGTGGAATATGGAGAGGCTTGGATTCGGGAACATGTATGTTCATATTATGACATTTATTCTGTTTTGCATAGCTCCGTTCTGGATTTTTAACTTGGCTGCTGTTAATGTCGACAATGAAACTGTTCGGGGGGCTCTTGGAGTTACTGGCCTAGTTCTATGTCTATTTGGTTTGCTCTATGGTGGCTTTTGGAGGATCCAAATGAGAAAGAGATTCAATTTGCCTTCTTATAACTTCTGCTTTGGTAAACCATCACTCTCTGATTGCACGTTGTGGCTTTGCTGTAGTTGGTGCTCTCTTGCTCAGGAAGTGAGGACCGGGAATTCCTATGACGTTGTGGAAGAAAAGTTTTATAGAAAACAACAGGAAAGTGAAAGCCTTCCACCAATTTCACCTTTGCCTCGTGAAGATGGATCGCTTGAGTTTAGATCTGGTCCGAATTCTCCTCTGGGGAACTACTTAAGCCCAACCAAGAATGGCATCGGTAGTTCTCTGAGTCCAAGCAGACTTTCAGCAGTGAAAGAAGAGTCCCATGTAAGAGGTAAGGATGAAACTATGACCCCACCTGCGCCTTCATTGATACAAAGGGAAGAAACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2985 PLAC8

Protein Analysis

441

Amino Acids

49.83

Weight (kDa)

8.77

Isoelectric Point (pI)

41.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2985 PF11204 4 - 82 2.2e-30 Protein of unknown function (DUF2985)
PLAC8 PF04749 216 - 346 6.3e-20 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012162)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10980 AT5G05350
fragaria_vesca FvH4_7g25461
malus_domestica MD01G1161000.v1.1 MD07G1229000.v1.1
prunus_persica Prupe.2G259100_v2.0.a1
pyrus_communis pycom01g17680 pycom07g20720
rosa_chinensis RchiOBHm_Chr1g0371431
rosa_laevigata RLG00000026963
rosa_multiflora Rmu_sc0000809.1_g000023 Rmu_sc0017835.1_g000001
rosa_roxburghii Rroxscaffold_4G00285690
rosa_rugosa Rorug01G0363900
rosa_samantha Rh1AG374100 Rh1BG337500 Rh1CG350800 Rh1DG368500
rosa_wichuraiana Rw1G032900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 300, 954
AarI CACCTGC 2 cut(s) 466, 1297
Acc36I ACCTGC 2 cut(s) 466, 1297
AccB7I CCANNNNNTGG 1 cut(s) 325
AccBSI CCGCTC 1 cut(s) 651
AccI GTMKAC 1 cut(s) 834
AciI CCGC 1 cut(s) 651
AclWI GGATC 4 cut(s) 59, 913, 926, 1144
AcsI RAATTY 3 cut(s) 165, 1045, 1162
AdeI CACNNNGTG 1 cut(s) 996
AfaI GTAC 3 cut(s) 200, 231, 660
AfiI CCNNNNNNNGG 5 cut(s) 312, 313, 325, 419, 499
AflII CTTAAG 1 cut(s) 1183
AflIII ACRYGT 1 cut(s) 756
AgsI TTSAA 3 cut(s) 109, 152, 940
AjnI CCWGG 1 cut(s) 387
AjuI GAANNNNNNNTTGG 2 cut(s) 1216, 1248
AluBI AGCT 2 cut(s) 404, 797
AluI AGCT 2 cut(s) 404, 797
Alw21I GWGCWC 3 cut(s) 406, 457, 1019
Alw44I GTGCAC 1 cut(s) 453
AlwI GGATC 4 cut(s) 59, 913, 926, 1144
Ama87I CYCGRG 1 cut(s) 494
AoxI GGCC 2 cut(s) 315, 871
ApaI GGGCCC 1 cut(s) 319
ApaLI GTGCAC 1 cut(s) 453
ApeKI GCWGC 1 cut(s) 821
ApoI RAATTY 3 cut(s) 165, 1045, 1162
Asp700I GAANNNNTTC 4 cut(s) 215, 595, 846, 1101
AspLEI GCGC 1 cut(s) 1294
AspS9I GGNCC 4 cut(s) 315, 316, 1038, 1157
AsuC2I CCSGG 1 cut(s) 1042
AsuHPI GGTGA 2 cut(s) 563, 1107
AsuII TTCGAA 1 cut(s) 271
AvaI CYCGRG 1 cut(s) 494
AvaII GGWCC 2 cut(s) 1038, 1157
BaeGI GKGCMC 2 cut(s) 319, 457
BamHI GGATCC 1 cut(s) 918
BanII GRGCYC 3 cut(s) 319, 406, 859
BarI GAAGNNNNNNTAC 2 cut(s) 203, 235
BauI CACGAG 1 cut(s) 1125
BbsI GAAGAC 1 cut(s) 261
Bbv12I GWGCWC 3 cut(s) 406, 457, 1019
BbvI GCAGC 1 cut(s) 808
BccI CCATC 5 cut(s) 213, 237, 319, 982, 1127
BciT130I CCWGG 1 cut(s) 389
BcnI CCSGG 1 cut(s) 1042
BfaI CTAG 1 cut(s) 875
BfmI CTRYAG 2 cut(s) 684, 1006
BfrI CTTAAG 1 cut(s) 1183
BfuAI ACCTGC 2 cut(s) 466, 1297
BglII AGATCT 1 cut(s) 1151
BisI GCNGC 1 cut(s) 822
BlsI GCNGC 1 cut(s) 823
BmcAI AGTACT 1 cut(s) 660
Bme1390I CCNGG 2 cut(s) 389, 1042
Bme18I GGWCC 2 cut(s) 1038, 1157
BmeT110I CYCGRG 1 cut(s) 494
BmgT120I GGNCC 4 cut(s) 315, 316, 1038, 1157
BmiI GGNNCC 3 cut(s) 317, 537, 920
BmrFI CCNGG 2 cut(s) 389, 1042
BmsI GCATC 2 cut(s) 130, 1212
BpiI GAAGAC 1 cut(s) 261
Bpu10I CCTNAGC 1 cut(s) 1026
Bpu14I TTCGAA 1 cut(s) 271
BpuEI CTTGAG 1 cut(s) 1163
BpuMI CCSGG 1 cut(s) 1042
BsaJI CCNNGG 2 cut(s) 388, 493
Bsc4I CCNNNNNNNGG 5 cut(s) 312, 313, 325, 419, 499
Bse1I ACTGG 3 cut(s) 103, 553, 874
BseBI CCWGG 1 cut(s) 389
BseDI CCNNGG 2 cut(s) 388, 493
BseGI GGATG 4 cut(s) 205, 526, 679, 1276
BseLI CCNNNNNNNGG 5 cut(s) 312, 313, 325, 419, 499
BseMII CTCAG 3 cut(s) 377, 1040, 1208
BseNI ACTGG 3 cut(s) 103, 553, 874
BseRI GAGGAG 2 cut(s) 669, 1158
BseSI GKGCMC 2 cut(s) 319, 457
BseXI GCAGC 1 cut(s) 808
BshFI GGCC 2 cut(s) 317, 873
BsiHKAI GWGCWC 3 cut(s) 406, 457, 1019
BsiHKCI CYCGRG 1 cut(s) 494
BsiSI CCGG 1 cut(s) 1041
BslFI GGGAC 3 cut(s) 115, 307, 1237
BslI CCNNNNNNNGG 5 cut(s) 312, 313, 325, 419, 499
BsmFI GGGAC 3 cut(s) 115, 307, 1237
BsnI GGCC 2 cut(s) 317, 873
BsoBI CYCGRG 1 cut(s) 494
Bsp119I TTCGAA 1 cut(s) 271
Bsp120I GGGCCC 1 cut(s) 315
Bsp1286I GDGCHC 5 cut(s) 319, 406, 457, 859, 1019
Bsp1407I TGTACA 1 cut(s) 198
Bsp143I GATC 4 cut(s) 51, 918, 1136, 1151
BspACI CCGC 1 cut(s) 651
BspANI GGCC 2 cut(s) 317, 873
BspCNI CTCAG 3 cut(s) 376, 1039, 1209
BspLI GGNNCC 3 cut(s) 317, 537, 920
BspMI ACCTGC 2 cut(s) 466, 1297
BspPI GGATC 4 cut(s) 59, 913, 926, 1144
BspQI GCTCTTC 1 cut(s) 394
BspT104I TTCGAA 1 cut(s) 271
BspTI CTTAAG 1 cut(s) 1183
BsrBI CCGCTC 1 cut(s) 651
BsrGI TGTACA 1 cut(s) 198
BsrI ACTGG 3 cut(s) 103, 553, 874
BssECI CCNNGG 2 cut(s) 388, 493
BssMI GATC 4 cut(s) 51, 918, 1136, 1151
BssSI CACGAG 1 cut(s) 1125
Bst2BI CACGAG 1 cut(s) 1125
Bst2UI CCWGG 1 cut(s) 389
Bst4CI ACNGT 2 cut(s) 284, 847
Bst6I CTCTTC 2 cut(s) 394, 1242
BstAFI CTTAAG 1 cut(s) 1183
BstAUI TGTACA 1 cut(s) 198
BstBI TTCGAA 1 cut(s) 271
BstC8I GCNNGC 1 cut(s) 469
BstDEI CTNAG 3 cut(s) 363, 1026, 1217
BstENI CCTNNNNNAGG 1 cut(s) 497
BstF5I GGATG 4 cut(s) 205, 526, 679, 1276
BstHHI GCGC 1 cut(s) 1294
BstKTI GATC 4 cut(s) 54, 921, 1139, 1154
BstMBI GATC 4 cut(s) 51, 918, 1136, 1151
BstMWI GCNNNNNNNGC 3 cut(s) 44, 368, 452
BstNI CCWGG 1 cut(s) 389
BstNSI RCATGY 1 cut(s) 760
BstSCI CCNGG 2 cut(s) 387, 1040
BstSFI CTRYAG 2 cut(s) 684, 1006
BstSLI GKGCMC 2 cut(s) 319, 457
BstV1I GCAGC 1 cut(s) 808
BstV2I GAAGAC 1 cut(s) 261
BstX2I RGATCY 2 cut(s) 918, 1151
BstYI RGATCY 2 cut(s) 918, 1151
BsuRI GGCC 2 cut(s) 317, 873
BtsCI GGATG 4 cut(s) 205, 526, 679, 1276
BtsI GCAGTG 1 cut(s) 1245
BtsIMutI CAGTG 2 cut(s) 546, 1245
BveI ACCTGC 2 cut(s) 466, 1297
Cac8I GCNNGC 1 cut(s) 469
CfoI GCGC 1 cut(s) 1294
Cfr13I GGNCC 4 cut(s) 315, 316, 1038, 1157
Csp6I GTAC 3 cut(s) 199, 230, 659
CviAII CATG 5 cut(s) 34, 144, 322, 757, 1256
CviQI GTAC 3 cut(s) 199, 230, 659
DdeI CTNAG 3 cut(s) 363, 1026, 1217
DpnI GATC 4 cut(s) 53, 920, 1138, 1153
DpnII GATC 4 cut(s) 51, 918, 1136, 1151
DraI TTTAAA 1 cut(s) 384
DraIII CACNNNGTG 1 cut(s) 996
Eam1104I CTCTTC 2 cut(s) 394, 1242
EarI CTCTTC 2 cut(s) 394, 1242
Ecl136II GAGCTC 1 cut(s) 404
Eco24I GRGCYC 3 cut(s) 319, 406, 859
Eco47I GGWCC 2 cut(s) 1038, 1157
Eco53kI GAGCTC 1 cut(s) 404
Eco88I CYCGRG 1 cut(s) 494
EcoICRI GAGCTC 1 cut(s) 404
EcoNI CCTNNNNNAGG 1 cut(s) 497
EcoRI GAATTC 2 cut(s) 1045, 1162
EcoRII CCWGG 1 cut(s) 387
EcoT22I ATGCAT 1 cut(s) 145
EcoT38I GRGCYC 3 cut(s) 319, 406, 859
FaeI CATG 5 cut(s) 37, 147, 325, 760, 1259
FaqI GGGAC 3 cut(s) 115, 307, 1237
FatI CATG 5 cut(s) 33, 143, 321, 756, 1255
FblI GTMKAC 1 cut(s) 834
Fnu4HI GCNGC 1 cut(s) 822
FokI GGATG 4 cut(s) 192, 533, 686, 1283
FriOI GRGCYC 3 cut(s) 319, 406, 859
Fsp4HI GCNGC 1 cut(s) 822
FspBI CTAG 1 cut(s) 875
GlaI GCGC 1 cut(s) 1293
GluI GCNGC 1 cut(s) 822
HaeIII GGCC 2 cut(s) 317, 873
HapII CCGG 1 cut(s) 1041
HhaI GCGC 1 cut(s) 1294
Hin1II CATG 5 cut(s) 37, 147, 325, 760, 1259
Hin6I GCGC 1 cut(s) 1292
HinP1I GCGC 1 cut(s) 1292
HincII GTYRAC 1 cut(s) 835
HindII GTYRAC 1 cut(s) 835
HinfI GANTC 6 cut(s) 216, 624, 747, 936, 1219, 1250
HpaII CCGG 1 cut(s) 1041
HphI GGTGA 2 cut(s) 563, 1107
Hpy166II GTNNAC 5 cut(s) 280, 455, 638, 835, 971
Hpy188I TCNGA 5 cut(s) 210, 351, 985, 1161, 1218
Hpy188III TCNNGA 6 cut(s) 264, 496, 751, 805, 1028, 1127
Hpy8I GTNNAC 5 cut(s) 280, 455, 638, 835, 971
HpyAV CCTTC 5 cut(s) 493, 548, 957, 1112, 1305
HpyCH4III ACNGT 2 cut(s) 284, 847
HpyCH4IV ACGT 2 cut(s) 992, 1056
HpyCH4V TGCA 9 cut(s) 77, 143, 371, 428, 455, 461, 605, 792, 990
HpyF10VI GCNNNNNNNGC 3 cut(s) 44, 368, 452
HpyF3I CTNAG 3 cut(s) 363, 1026, 1217
HpySE526I ACGT 2 cut(s) 992, 1056
Hsp92II CATG 5 cut(s) 37, 147, 325, 760, 1259
HspAI GCGC 1 cut(s) 1292
Kzo9I GATC 4 cut(s) 51, 918, 1136, 1151
LguI GCTCTTC 1 cut(s) 394
LmnI GCTCC 3 cut(s) 557, 648, 802
Lsp1109I GCAGC 1 cut(s) 808
LweI GCATC 2 cut(s) 130, 1212
MaeI CTAG 1 cut(s) 875
MaeII ACGT 2 cut(s) 992, 1056
MaeIII GTNAC 2 cut(s) 392, 865
MalI GATC 4 cut(s) 53, 920, 1138, 1153
MbiI CCGCTC 1 cut(s) 651
MboI GATC 4 cut(s) 51, 918, 1136, 1151
MboII GAAGA 5 cut(s) 266, 411, 1076, 1142, 1259
MflI RGATCY 2 cut(s) 918, 1151
MhlI GDGCHC 5 cut(s) 319, 406, 457, 859, 1019
MluCI AATT 8 cut(s) 78, 165, 352, 566, 940, 1045, 1110, 1162
MlyI GAGTC 3 cut(s) 633, 1228, 1259
MnlI CCTC 9 cut(s) 503, 517, 647, 732, 909, 1029, 1134, 1179, 1256
Mph1103I ATGCAT 1 cut(s) 145
MroXI GAANNNNTTC 4 cut(s) 215, 595, 846, 1101
MseI TTAA 5 cut(s) 383, 569, 813, 828, 1184
MspCI CTTAAG 1 cut(s) 1183
MspI CCGG 1 cut(s) 1041
MspR9I CCNGG 2 cut(s) 389, 1042
MssI GTTTAAAC 1 cut(s) 384
MvaI CCWGG 1 cut(s) 389
MwoI GCNNNNNNNGC 3 cut(s) 44, 368, 452
NciI CCSGG 1 cut(s) 1042
NdeII GATC 4 cut(s) 51, 918, 1136, 1151
NlaIII CATG 5 cut(s) 37, 147, 325, 760, 1259
NlaIV GGNNCC 3 cut(s) 317, 537, 920
NsiI ATGCAT 1 cut(s) 145
NspI RCATGY 1 cut(s) 760
NspV TTCGAA 1 cut(s) 271
PaqCI CACCTGC 2 cut(s) 466, 1297
PciI ACATGT 1 cut(s) 756
PciSI GCTCTTC 1 cut(s) 394
PdmI GAANNNNTTC 4 cut(s) 215, 595, 846, 1101
PfeI GAWTC 3 cut(s) 216, 747, 936
PflMI CCANNNNNTGG 1 cut(s) 325
PkrI GCNGC 1 cut(s) 823
PleI GAGTC 3 cut(s) 632, 1227, 1258
PmeI GTTTAAAC 1 cut(s) 384
PpsI GAGTC 3 cut(s) 632, 1227, 1258
PscI ACATGT 1 cut(s) 756
PsiI TTATAA 2 cut(s) 300, 954
Psp124BI GAGCTC 1 cut(s) 406
Psp6I CCWGG 1 cut(s) 387
PspGI CCWGG 1 cut(s) 387
PspN4I GGNNCC 3 cut(s) 317, 537, 920
PspOMI GGGCCC 1 cut(s) 315
PspPI GGNCC 4 cut(s) 315, 316, 1038, 1157
PsuI RGATCY 2 cut(s) 918, 1151
RsaI GTAC 3 cut(s) 200, 231, 660
RsaNI GTAC 3 cut(s) 199, 230, 659
SacI GAGCTC 1 cut(s) 406
SalI GTCGAC 1 cut(s) 833
SapI GCTCTTC 1 cut(s) 394
SaqAI TTAA 5 cut(s) 383, 569, 813, 828, 1184
SatI GCNGC 1 cut(s) 822
Sau3AI GATC 4 cut(s) 51, 918, 1136, 1151
Sau96I GGNCC 4 cut(s) 315, 316, 1038, 1157
ScaI AGTACT 1 cut(s) 660
SchI GAGTC 3 cut(s) 633, 1228, 1259
ScrFI CCNGG 2 cut(s) 389, 1042
SduI GDGCHC 5 cut(s) 319, 406, 457, 859, 1019
SfaNI GCATC 2 cut(s) 130, 1212
SfcI CTRYAG 2 cut(s) 684, 1006
SfuI TTCGAA 1 cut(s) 271
SinI GGWCC 2 cut(s) 1038, 1157
SmlI CTYRAG 2 cut(s) 1142, 1183
SmoI CTYRAG 2 cut(s) 1142, 1183
Sse9I AATT 8 cut(s) 78, 165, 352, 566, 940, 1045, 1110, 1162
SsiI CCGC 1 cut(s) 651
SspI AATATT 1 cut(s) 596
SspMI CTAG 1 cut(s) 875
SstI GAGCTC 1 cut(s) 406
StyD4I CCNGG 2 cut(s) 387, 1040
TaaI ACNGT 2 cut(s) 284, 847
TaiI ACGT 2 cut(s) 995, 1059
TaqI TCGA 2 cut(s) 271, 834
TasI AATT 8 cut(s) 78, 165, 352, 566, 940, 1045, 1110, 1162
TatI WGTACW 3 cut(s) 198, 229, 658
TfiI GAWTC 3 cut(s) 216, 747, 936
Tru1I TTAA 5 cut(s) 383, 569, 813, 828, 1184
Tru9I TTAA 5 cut(s) 383, 569, 813, 828, 1184
TscAI CASTG 2 cut(s) 553, 1245
TseI GCWGC 1 cut(s) 821
TspDTI ATGAA 6 cut(s) 44, 335, 755, 855, 1287, 1287
TspGWI ACGGA 1 cut(s) 789
TspRI CASTG 2 cut(s) 553, 1245
Van91I CCANNNNNTGG 1 cut(s) 325
Vha464I CTTAAG 1 cut(s) 1183
VneI GTGCAC 1 cut(s) 453
VpaK11BI GGWCC 2 cut(s) 1038, 1157
XagI CCTNNNNNAGG 1 cut(s) 497
XapI RAATTY 3 cut(s) 165, 1045, 1162
XceI RCATGY 1 cut(s) 760
XmiI GTMKAC 1 cut(s) 834
XmnI GAANNNNTTC 4 cut(s) 215, 595, 846, 1101
XspI CTAG 1 cut(s) 875
ZrmI AGTACT 1 cut(s) 660
Zsp2I ATGCAT 1 cut(s) 145
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.